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https://www.readbyqxmd.com/read/28453253/athabasca-oil-sands-petcoke-extract-elicits-biochemical-and-transcriptomic-effects-in-avian-hepatocytes
#1
Doug Crump, Kim Williams, Suzanne Chiu, Yifeng Zhang, Jonathan W Martin
Petroleum coke or "petcoke" is a granular carbonaceous material produced during the upgrading of heavy crude oils, including bitumen. Petcoke dust was recently reported as an environmental contaminant in the Athabasca oil sands region, but the ecotoxicological hazards posed by this complex bitumen-derived material - including those to avian species - have not been characterized. In this study, solvent extracts (x) of delayed and fluid petcoke (xDP and xFP) were prepared and dissolved in dimethylsulfoxide. A water accommodated fraction of delayed petcoke (waDP) was also prepared...
April 28, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28449108/poly-a-clickseq-click-chemistry-for-next-generation-3%C3%AE-end-sequencing-without-rna-enrichment-or-fragmentation
#2
Andrew Routh, Ping Ji, Elizabeth Jaworski, Zheng Xia, Wei Li, Eric J Wagner
The recent emergence of alternative polyadenylation (APA) as an engine driving transcriptomic diversity has stimulated the development of sequencing methodologies designed to assess genome-wide polyadenylation events. The goal of these approaches is to enrich, partition, capture and ultimately sequence poly(A) site junctions. However, these methods often require poly(A) enrichment, 3΄ linker ligation steps, and RNA fragmentation, which can necessitate higher levels of starting RNA, increase experimental error and potentially introduce bias...
April 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28442387/establishment-of-microrna-delivery-system-by-pp7-bacteriophage-like-particles-carrying-cell-penetrating-peptide
#3
Yanli Sun, Yanhua Sun, Ronglan Zhao
MicroRNAs have great therapeutic potential in cancer and other diseases. However, their instability and low in vivo delivery efficiency limits their application. Recombinant PP7 bacteriophage-based virus-like particles (VLPs) could protect microRNAs against rapid degradation by RNase by packaging specific exogenous pre-microRNAs using the pac site. Insertion of a cell-penetrating peptide (CPP) into the AB-loop of VLPs could significantly improve the delivery efficiency of microRNAs into mammalian cells. Unlike other microRNA delivery methods (viral or non-viral vectors), recombinant PP7 VLPs carrying a CPP and microRNA could be efficiently expressed in Escherichia coli using the one-plasmid double expression system...
April 22, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28438503/expanding-role-of-certified-electronic-health-records-technology-in-radiology-the-macra-mandate
#4
Gregory N Nicola, Andrew B Rosenkrantz, Joshua A Hirsch, Ezequiel Silva, Keith J Dreyer, Michael P Recht
Radiology has historically been at the forefront of innovation and the advancement of technology for the benefit of patient care. However, challenges to early implementation prevented most radiologists from adopting and integrating certified electronic health record technology (CEHRT) into their daily workflow despite the early and potential advantages it offered. This circumstance places radiology at a disadvantage in the two payment pathways of the Medicare Access and CHIP Reauthorization Act of 2015: the Merit-Based Incentive Payment System (MIPS) and advanced alternative payment models (APMs)...
April 19, 2017: Journal of the American College of Radiology: JACR
https://www.readbyqxmd.com/read/28436586/transesophageal-and-invasive-electrophysiologic-evaluation-in-children-with-wolff-parkinson-white-pattern
#5
Serhat Koca, Feyza Aysenur Pac, Ahmet Vedat Kavurt, Serkan Cay, Ajda Mihcioglu, Dursun Aras, Serkan Topaloglu
BACKGROUND: Risk stratification for Wolff-Parkinson-White (WPW) pattern either by non-invasive or invasive tests is important to determine whether an ablation is necessary or not. The aim was to compare non-invasive tests and invasive studies in a pediatric WPW population. METHODS: A total of 71 WPW patients [median age 14 years (IQR 11 - 16 years); 43 male] underwent Holter monitoring, exercise stress test (EST), and transesophageal electrophysiological study (TEEPS)...
April 24, 2017: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/28433767/temporal-dynamics-of-human-excreted-pollutants-in-wastewater-treatment-plant-influents-toward-a-better-knowledge-of-mass-load-fluctuations
#6
Thomas Thiebault, Laëtitia Fougère, Emilie Destandau, Maxime Réty, Jérémy Jacob
The occurrence of 25 drug target residues (illicit drugs or pharmaceutically active compounds) was investigated during 85 consecutive days in the influents of a wastewater treatment plant in the Region Centre-Val de Loire, France. This long tracking period allowed a better understanding of the patterns affecting the occurrence of this type of contaminants. Among them, 2 were never detected (i.e. heroin and amphetamine). Concerning illicit drugs two patterns were found. Cocaine and ecstasy median loads varied considerably between weekdays and weekend days (i...
April 20, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28429324/photomovement-in-euglena
#7
Donat-P Häder, Mineo Iseki
Motile microorganisms such as the green Euglena gracilis use a number of external stimuli to orient in their environment. They respond to light with photophobic responses, photokinesis and phototaxis, all of which can result in accumulations of the organisms in suitable habitats. The light responses operate synergistically with gravitaxis, aerotaxis and other responses. Originally the microscopically obvious stigma was thought to be the photoreceptor, but later the paraxonemal body (PAB, paraflagellar body) has been identified as the light responsive organelle, located in the trailing flagellum inside the reservoir...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28427942/inhibitory-effects-of-ethyl-pyruvate-on-platelet-aggregation-and-phosphatidylserine-exposure
#8
Wenjin Li, Xinyu Yang, Minyuan Peng, Can Li, Guangfu Mu, Fangping Chen
Ethyl pyruvate (EP) is a stable lipophilic pyruvate derivative. Studies demonstrated that EP shows potent anti-oxidation, anti-inflammatory and anti-coagulant effects. Inflammation and coagulation are closely interacted with platelet activation. However, it is unclear whether EP has anti-platelet effects. Therefore, we investigated the anti-platelet effect of EP in this study in vitro. We found that EP inhibited agonists induced platelets aggregation, ATP release and adhesion to collagen. Flow cytometric analysis revealed that EP inhibited agonist induced platelets PAC-1 binding, as well as P-selectin and CD40L expression...
April 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28426230/photophysics-and-excited-state-properties-of-cyclometalated-iridium-iii-platinum-ii-and-iridium-iii-iridium-iii-bimetallic-complexes-bridged-by-dipyridylpyrazine
#9
Yang-Jin Cho, So-Yoen Kim, Chang Min Choi, Nam Joon Kim, Chul Hoon Kim, Dae Won Cho, Ho-Jin Son, Chyongjin Pac, Sang Ook Kang
We investigated the electrochemical and excited-state properties of 2,3-bis(2-pyridyl)pyrazine (dpp)-bridged bimetallic complexes, (L)2Ir-dpp-PtCl [1, L = 2-(4',6'-difluorophenyl)pyridinato-N,C(2) (dfppy); 2, L = 2-phenylpyridinato-N,C(2) (ppy)] and [(L)2Ir]2(dpp) [3, L = dfppy; 4, L = ppy] compared to monometallic complexes, (L)2Ir-dpp (5, L = dfppy; 6, L = ppy) and dpp-PtCl (dpp-Pt(II)Cl2; 7). The single-crystal X-ray crystallographic structures of 1, 3, 5, and 6 showed that 1 and 3 have approximately coplanar structures of the dpp unit, while the noncoordinated pyridine ring of dpp in 5 and 6 is largely twisted with respect to the pyrazine ring...
April 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28424890/left-atrial-electromechanical-conduction-time-predicts-atrial-fibrillation-in-patients-with-mitral-stenosis-a-5-year-follow-up-speckle-tracking-echocardiography-study
#10
Ozkan Candan, Cetin Gecmen, Arzu Kalayci, Cem Dogan, Emrah Bayam, Mehmet Ozkan
Prolonged left atrial electromechanical conduction time is related with atrial electrical remodeling, and is predictive of the development of atrial fibrillation. The aim of our study was to examine whether left atrial electromechanical conduction time (EMT) and left atrial strain as measured by speckle tracking echocardiography (STE) are predictors for the development of atrial fibrillation (AF) in patients with mitral stenosis (MS) at 5-year follow-up. A total of 81 patients (61% females; mean age 38.1 ± 12...
April 19, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28424442/clinical-improvement-and-no-further-need-of-transplant-after-closure-of-pda-with-transcatheter-approach-in-an-end-stage-heart-failure-patient-with-hypertrabeculation
#11
Serhat Koca, Feyza Ayşenur Paç, Ajda Mutlu Mıhçıoğlu, Vedat Kavurt, Denizhan Bağrul
Although heart failure is managed medically most of the time, heart transplantation is still last resort for selected end-stage heart failure patients with noncompaction cardiomyopathy. Presently described for the first time is case of pediatric patient with noncompaction cardiomyopathy who was initially referred to our hospital for heart transplant but underwent PDA repair and improved clinically without need for heart transplant.
March 2017: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
https://www.readbyqxmd.com/read/28423751/web-validation-service-for-ensuring-adherence-to-the-dicom-standard
#12
Jorge Miguel Silva, Tiago Marques Godinho, David Silva, Carlos Costa
The DICOM Standard has been fundamental for ensuring the interoperability of Picture Archive and Communications Systems (PACS). By compiling rigorously to the standard, medical imaging equipment and applications from different vendors can share their data, and create integrated workflows which contributes to better quality healthcare services. However, DICOM is a complex, flexible and very extensive standard. Thus, it is difficult to attest the conformity of data structures produced by DICOM applications resulting in unexpected behaviors, errors and malfunctions...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28422891/relationship-and-associated-mechanisms-between-ambulatory-blood-pressure-and-clinic-blood-pressure-with-prevalent-cardiovascular-disease-in-diabetic-hypertensive-patients
#13
Zirui Hao, Guiping Li, Yue Sun, Yan Liu
The present study was to compare the association between ambulatory blood pressure (ABP) and clinic BP (CBP) with prevalent cardiovascular diseases (CVD); and the underlying mechanism would also be investigated concurrently.Diabetic hypertensive patients were enrolled and divided into 2 groups based on presence of CVD. Twenty-four hour-ABP monitoring was performed and between-group differences were evaluated and logistic regression analysis was conducted.A total of 568 diabetic hypertensive patients were enrolled, and the mean age was 60...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28420208/serum-insulin-like-growth-factor-axis-and-the-risk-of-pancreatic-cancer-systematic-review-and-meta-analysis
#14
Yuanfeng Gong, Bingyi Zhang, Yadi Liao, Yunqiang Tang, Cong Mai, Tiejun Chen, Hui Tang
OBJECTIVE: To investigate the association between serum concentration of insulin-like growth factor (IGF) and the risk of pancreatic cancer (PaC). METHODS: We identified eligible studies in Medline and EMBASE databases (no reference trials from 2014 to 2016) in addition to the reference lists of original studies and review articles on this topic. A summary of relative risks with 95% confidence intervals (CI) was calculated using a random-effects model. The heterogeneity between studies was assessed using Cochran Q and I² statistics...
April 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28418655/accuracy-test-of-the-opls-aa-force-field-for-calculating-free-energies-of-mixing-and-comparison-with-pac-mac
#15
Augustinus J M Sweere, Johannes G E M Fraaije
We have calculated the excess free energy of mixing of 1053 binary mixtures with the OPLS-AA force field using two different methods: thermodynamic integration (TI) of molecular dynamics simulations and the Pair Configuration to Molecular Activity Coefficient (PAC-MAC) method. PAC-MAC is a force field based quasi-chemical method for predicting miscibility properties of various binary mixtures. The TI calculations yield a root mean squared error (RMSE) compared to experimental data of 0.132 kBT (0.37 kJ/mol)...
April 24, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28415757/an-absence-of-platelet-activation-following-thalidomide-treatment-in-vitro-or-in-vivo
#16
Jianlin Qiao, Yulu Wu, Xiaoqing Wu, Yun Liu, Xiaoqian Li, Wen Ju, Kunming Qi, Depeng Li, Elizabeth E Gardiner, Robert K Andrews, Lingyu Zeng, Kailin Xu
Increased risk of thromboembolism and platelet hyperreactivity has been reported in patients receiving thalidomide therapy. Whether thalidomide induces platelet activation directly or through other factors remains unclear. The aim of this study was to evaluate the effect of thalidomide on platelet activation under resting conditions in vitro and in vivo. Isolated human or mouse platelets were treated with different concentrations of thalidomide (10, 50 and 100 μg/ml) for 60 min at 37°C followed by analysis of platelet surface expression of platelet receptors GPIbα, GPVI, αIIbβ3 and P-selectin, and PAC-1 or fibrinogen binding, by flow cytometry and collagen- or ADP-induced platelet aggregation...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414123/anti-factor-xa-activities-of-zingerone-with-anti-platelet-aggregation-activity
#17
Wonhwa Lee, Sae-Kwang Ku, Mi-Ae Kim, Jong-Sup Bae
Zingerone (ZGR), a phenolic alkanone found in Zingiber officinale, has been reported to have various pharmacological activities such as anti-inflammatory, anti-apoptotic, and protecting myocardial infarction and irritable bowel disorder. The aim was to identify the unreported bioactive anti-factor Xa (FXa) and anti-platelet activities of ZGR. ZGR was evaluated for their anti-FXa and anti-platelet aggregation properties by monitoring clotting time, platelet aggregation, FXa activity and production, and thrombus formation...
April 13, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28412061/influence-of-the-labrum-on-version-and-diameter-of-the-glenoid-a-morphometric-study-using-magnetic-resonance-images
#18
Joyce Anthony, Ibin Varughese, Vaida Glatt, Kevin Tetsworth, Erik Hohmann
PURPOSE: To use magnetic resonance imaging to determine the influence of the labrum on both the osseous version and effective diameter of the glenoid. METHODS: This was a retrospective, cross-sectional study of patients with shoulder pain who underwent MRI between February 2014 and February 2015. The morphology of the glenoid labrum and glenoid was scanned with a 3-T magnetic resonance imaging scanner, and variables were measured by use of IntelliSpace PACS Enterprise...
April 12, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28409269/short-term-outcomes-of-laparoscopic-ventral-rectopexy-for-obstructed-defecation-in-patients-with-overt-pelvic-structural-abnormalities-a-chinese-pilot-study
#19
G Y Ye, Z Wang, K E Matzel, Z Cui
AIM: As laparoscopic ventral rectopexy (LVR) gained increasing popularity in the past decade, studies from non-western area remain rare. The aim of this pilot study is to evaluate the efficacy and safety of LVR for obstructed defecation (OD) in Chinese patients with overt pelvic structural abnormalities. METHODS: A series of 19 consecutive patients is presented undergoing LVR for OD. All patients showed various forms of pelvic structural abnormalities which were verified by dynamic defecography exam...
April 13, 2017: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/28407440/improving-education-and-supervision-of-queensland-x-ray-operators-through-video-conference-technology-a-teleradiography-pilot-project
#20
Marnie Rawle, Tanya Oliver, Alison Pighills, Daniel Lindsay
INTRODUCTION: X-ray Operator (XO) supervision in Queensland is performed by radiographers in a site removed from the XO site. This has historically been performed by telephone when the XO requires immediate help, as well as post-examination through radiographer review and the provision of written feedback on images produced. This project aimed to improve image quality through the provision of real-time support of XOs by the introduction of video conference (VC) supervision. METHODS: A 6-month pilot project compared image quality with and without VC supervision...
April 13, 2017: Journal of Medical Radiation Sciences
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