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https://www.readbyqxmd.com/read/29055260/swimming-exercise-reverses-cums-induced-changes-in-depression-like-behaviors-and-hippocampal-plasticity-related-proteins
#1
Weina Liu, Xiangli Xue, Jie Xia, Jiatong Liu, Zhengtang Qi
BACKGROUND: Stress-induced failed resilience of brain plasticity can contribute to the onset and recurrence of depression. Chronic stress has been reported to open windows of epigenetic plasticity in hippocampus. However, how hippocampal plasticity underlies depression-like behaviors and how it adapts in response to stress has not been addressed. The present study aimed to investigate the signaling mechanisms of CUMS affecting hippocampal plasticity-related proteins expression and the regulation of swimming exercise in mice...
October 6, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29055191/design-isolation-and-evaluation-of-the-binding-efficiency-of-a-dna-aptamer-against-interleukin-2-receptor-alpha-in-vitro
#2
Mahin Shahdordizadeh, Seyed Mohammad Taghdisi, Mojtaba Sankian, Mohammad Ramezani, Khalil Abnous
High levels of CD25, as part of the IL-2 receptor, are expressed on the surface of the activated T lymphocytes and regulatory T cells, indicating that the soluble CD25 (sCD25) could be a clinically valuable tool for treating several diseases. Moreover, progress has been achieved in targeting the IL-2 receptor to treat autoimmune diseases, organ transplantation and certain hematological malignancies. In the current study, generation of an ssDNA aptamer (Apt51) against CD25 is reported. Apt51 bound to CD25 with high affinity (Kd=13...
October 18, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29055189/a-mannose-specific-c-type-lectin-from-fenneropenaeus-merguiensis-exhibited-antimicrobial-activity-to-mediate-shrimp-innate-immunity
#3
Phanthipha Runsaeng, Peerapong Puengyam, Prapaporn Utarabhand
Being one type of pattern recognition receptors (PRRs), lectins exhibit a crucial role in the defense mechanism of invertebrates which are deficient in an adaptive immune system. A new C-type lectin called FmLC3 was isolated from hepatopancreas of Fenneropenaeus merguiensis by cloning approaches, RT-PCR and 5' and 3' RACE (rapid amplification of cDNA ends). A full-length cDNA of FmLC3 contains 607 bp with one open reading frame of 480bp, encoding a 159-amino acids peptide. The predicted primary structure of FmLC3 is composed of a signal peptide, a carbohydrate recognition domain with an EPN motif and one Ca(2+) binding site-2, including a double-loop region assisted by two conserved disulfide linkages...
October 18, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29055154/gene-expression-changes-in-male-accessory-glands-during-ageing-are-accompanied-by-reproductive-decline-in-drosophila-melanogaster
#4
Mareike Koppik, Claudia Fricke
Senescence is accompanied by loss of reproductive functions. Here we studied reproductive ageing in Drosophila melanogaster males and asked whether the expected decline in male reproductive success is due to diminished functionality of the male accessory gland. The male accessory gland produces the majority of seminal fluid proteins (SFPs) transferred to the female at mating. SFPs induce female post-mating changes and are key to male reproductive success. We measured age-dependent gene expression changes for five representative SFP genes in males from four different age groups ranging from one to six weeks after eclosion...
October 21, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29055152/predominance-of-regorafenib-over-sorafenib-restoration-of-membrane-bound-mica-in-hepatocellular-carcinoma-cells
#5
Jun Arai, Kaku Goto, Anthony Stephanou, Yasushi Tanoue, Sayaka Ito, Ryosuke Muroyama, Yasuo Matsubara, Ryo Nakagawa, Sayuri Morimoto, Yoshimi Kaise, Lay Ahyoung Lim, Hitoshi Yoshida, Naoya Kato
BACKGROUND AND AIM: The multi-kinase inhibitor regorafenib (REG) was recently demonstrated to be effective in patients with sorafenib (SOR)-resistant hepatocellular carcinoma (HCC). Interestingly, SOR is known to enhance the accumulation of membrane-bound MHC class I polypeptide-related sequence A (mMICA) in HCC cells, and to block production of soluble MICA (sMICA), an immunological decoy. In addition, MICA is associated with HCC in patients with chronic hepatitis C. We have now compared the impact of REG and SOR on MICA in HCC cells, as well as the immunotherapeutic implications thereof...
October 21, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29055138/effects-of-a-bone-graft-substitute-consisting-of-porous-gradient-ha-zro2-and-gelatin-chitosan-slow-release-hydrogel-containing-bmp-2-and-bmscs-on-lumbar-vertebral-defect-repair-in-rhesus-monkey
#6
Rong-Xue Shao, Ren-Fu Quan, Tuo Wang, Wei-Bin Du, Gao-Yong Jia, Dong Wang, Long-Bao Lv, Cai-Yin Xu, Xi-Cheng Wei, Jin-Fu Wang, Di-Sheng Yang
Dense biomaterial plays an important role in bone replacement. However, it fails to induce bone-cell migration into graft material. In the present study, a novel bone-graft substitute (BGS) consisting of porous gradient HA/ZrO2 composite (PGHC) and gelatin/chitosan slow-release hydrogel containing BMP-2 and BMSCs was designed and prepared to repair lumbar vertebral defects. The morphological characteristics of the BGS evaluated by scanning electron microscope showed that it had a three-dimensional network structure with uniformly distributed chitosan microspheres on the surfaces of the graft material and the interior of the pores...
October 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29055091/functional-interaction-of-junctophilin-2-with-small-conductance-ca-2-activated-potassium-channel-subtype-2-sk2-in-mouse-cardiac-myocytes
#7
Hong K Fan, Tian X Luo, Wei D Zhao, Yong H Mu, Yang Yang, Wen J Guo, Hui Y Tu, Qian Zhang
AIM: Junctophilins (JPs), a protein family of the junctional membrane complex, maintain the close conjunction between cell surface and intracellular membranes in striate muscle cells mediating the cross talk between extracellular Ca(2+) entry and intracellular Ca(2+) release. The small conductance Ca(2+) -activated K(+) channels are activated by the intracellular calcium and play an essential role in the cardiac action potential profile. Molecular mechanisms of regulation of the SK channels are still uncertain...
October 21, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/29055088/the-transcriptome-extracellular-proteome-and-active-secretome-of-agroinfiltrated-n-benthamiana-uncover-a-large-diverse-protease-repertoire
#8
Friederike Grosse-Holz, Steven Kelly, Svenja Blaskowski, Farnusch Kaschani, Markus Kaiser, Renier A L van der Hoorn
Infiltration of disarmed Agrobacterium tumefaciens into leaves of Nicotiana benthamiana (agroinfiltration) facilitates quick and safe production of antibodies, vaccines, enzymes and metabolites for industrial use (Molecular Farming). However, yield and purity of proteins produced by agroinfiltration are hampered by unintended proteolysis, restricting industrial viability of the agroinfiltration platform. Proteolysis may be linked to an immune response to agroinfiltration, but understanding of the response to agroinfiltration is limited...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29055073/ppnac1-a-main-regulator-of-phenylalanine-biosynthesis-and-utilization-in-maritime-pine
#9
Mª Belén Pascual, María-Teresa Llebrés, Blanca Craven-Bartle, Rafael A Cañas, Francisco M Cánovas, Concepción Ávila
The transcriptional regulation of phenylalanine metabolism is particularly important in conifers, long-lived species that use large amounts of carbon in wood. Here, we show that the Pinus pinaster transcription factor, PpNAC1, is a main regulator of phenylalanine biosynthesis and utilization. A phylogenetic analysis classified PpNAC1 in the NST proteins group, and was selected for functional characterization. PpNAC1 is predominantly expressed in the secondary xylem and compression wood of adult trees. Silencing of PpNAC1 in P...
October 21, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29055053/expression-patterns-of-ion-channels-and-structural-proteins-in-a-multimodal-cell-type-of-the-avian-optic-tectum
#10
Katharina Lischka, Simone Ladel, Harald Luksch, Stefan Weigel
The midbrain is an important subcortical area involved in distinct functions such as multimodal integration, movement initiation, bottom-up and top-down attention. Our group is particularly interested in cellular computation of multisensory integration. We focus on the visual part of the avian midbrain, the optic tectum (TeO, counterpart to mammalian superior colliculus). This area has a layered structure with the great advantage of distinct in- and output regions. In chicken, the TeO is organized in 15 layers where visual input targets the superficial layers while auditory input terminates in deeper layers...
October 21, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29055021/meercat-multiplexed-efficient-cell-free-expression-of-recombinant-qconcats-for-large-scale-absolute-proteome-quantification
#11
Nobuaki Takemori, Ayako Takemori, Yuki Tanaka, Yaeta Endo, Jane L Hurst, Guadalupe Gómez-Baena, Victoria M Harman, Robert J Beynon
A major challenge in proteomics is the absolute accurate quantification of large numbers of proteins. QconCATs, artificial proteins that are concatenations of multiple standard peptides, are well established as an efficient means to generate standards for proteome quantification. Previously, QconCATs have been expressed in bacteria, but we now describe QconCAT expression in a robust, cell-free system. The new expression approach rescues QconCATs that previously were unable to be expressed in bacteria and can reduce the incidence of proteolytic damage to QconCATs...
October 20, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29055014/protein-acyltransferase-dhhc3-regulates-breast-tumor-growth-oxidative-stress-and-senescence
#12
Martin E Hemler, Chandan Sharma, Hong-Xing Wang, Qinglin Li, Konstantin Knoblich, Emily S Reisenbichler, Andrea L Richardson
DHHC-type protein acyltransferases may regulate the localization, stability and/or activity of their substrates. In this study, we show that the protein palmitoyltransferase DHHC3 is upregulated in malignant and metastatic human breast cancer. Elevated expression of DHHC3 correlated with diminished patient survival in breast cancer and six other human cancer types. ZDHHC3 ablation in human MDA-MB-231 mammary tumor cell xenografts reduced the sizes of both the primary tumor and metastatic lung colonies. Gene array data and fluorescence dye assays documented increased oxidative stress and senescence in ZDHHC3-ablated cells...
October 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/29055013/a-synthetic-cd8%C3%AE-myd88-co-receptor-enhances-cd8-t-cell-responses-to-weakly-immunogenic-and-lowly-expressed-tumor-antigens
#13
Sabina Kaczanowska, Ann Mary Joseph, Jitao Guo, Alexander K Tsai, Jackline Joy M Lasola, Kenisha Younger, Yuji Zhang, Cruz Velasco Gonzalez, Eduardo Davila
T cell-based immunotherapies are a promising approach for patients with advanced cancers. However, various obstacles limit T cell efficacy, including suboptimal T cell receptor (TCR) activation and an immunosuppressive tumor environment. Here we developed a fusion protein by linking CD8α and MyD88 (CD8α:MyD88) to enhance CD8+ T cell responses to weakly immunogenic and poorly expressed tumor antigens. CD8α:MyD88-engineered T cells exhibited increased proliferation and expression of effector and co-stimulatory molecules in a tumor antigen-dependent manner...
October 20, 2017: Cancer Research
https://www.readbyqxmd.com/read/29055006/posttranscriptional-regulation-of-hla-a-protein-expression-by-alternative-polyadenylation-signals-involving-the-rna-binding-protein-syncrip
#14
Smita Kulkarni, Veron Ramsuran, Marijana Rucevic, Sukhvinder Singh, Alexandra Lied, Viraj Kulkarni, Colm O'hUigin, Sylvie Le Gall, Mary Carrington
Genomic variation in the untranslated region (UTR) has been shown to influence HLA class I expression level and associate with disease outcomes. Sequencing of the 3'UTR of common HLA-A alleles indicated the presence of two polyadenylation signals (PAS). The proximal PAS is conserved, whereas the distal PAS is disrupted within certain alleles by sequence variants. Using 3'RACE, we confirmed expression of two distinct forms of the HLA-A 3'UTR based on use of either the proximal or the distal PAS, which differ in length by 100 bp...
October 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29055005/induction-of-systemic-autoimmunity-by-a-xenobiotic-requires-endosomal-tlr-trafficking-and-signaling-from-the-late-endosome-and-endolysosome-but-not-type-i-ifn
#15
K Michael Pollard, Gabriela M Escalante, Hua Huang, Katarina M Haraldsson, Per Hultman, Joseph M Christy, Rahul D Pawar, Jessica M Mayeux, Rosana Gonzalez-Quintial, Roberto Baccala, Bruce Beutler, Argyrios N Theofilopoulos, Dwight H Kono
Type I IFN and nucleic acid-sensing TLRs are both strongly implicated in the pathogenesis of lupus, with most patients expressing IFN-induced genes in peripheral blood cells and with TLRs promoting type I IFNs and autoreactive B cells. About a third of systemic lupus erythematosus patients, however, lack the IFN signature, suggesting the possibility of type I IFN-independent mechanisms. In this study, we examined the role of type I IFN and TLR trafficking and signaling in xenobiotic systemic mercury-induced autoimmunity (HgIA)...
October 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29054998/interleukin-10-from-cd4-follicular-regulatory-t-cells-promotes-the-germinal-center-response
#16
Brian J Laidlaw, Yisi Lu, Robert A Amezquita, Jason S Weinstein, Jason A Vander Heiden, Namita T Gupta, Steven H Kleinstein, Susan M Kaech, Joe Craft
CD4(+) follicular regulatory T (Tfr) cells suppress B cell responses through modulation of follicular helper T (Tfh) cells and germinal center (GC) development. We found that Tfr cells can also promote the GC response through provision of interleukin-10 (IL-10) after acute infection with lymphocytic choriomeningitis virus (LCMV). Sensing of IL-10 by B cells was necessary for optimal development of the GC response. GC B cells formed in the absence of Treg cell-derived IL-10 displayed an altered dark zone state and decreased expression of the transcription factor Forkhead box protein 1 (FOXO1)...
October 20, 2017: Science Immunology
https://www.readbyqxmd.com/read/29054985/caveolae-mediated-endocytosis-as-a-novel-mechanism-of-resistance-to-trastuzumab-emtansine-t-dm1
#17
Matthew Sung, Xingzhi Tan, Bingwen Lu, Jonathan Golas, Christine Hosselet, Fang Wang, Laurie Tylaska, Lindsay King, Dahui Zhou, Russell Dushin, Jeremy S Myers, Edward Rosfjord, Judy Lucas, Hans-Peter Gerber, Frank Loganzo
Trastuzumab emtansine (T-DM1) is an antibody-drug conjugate (ADC) that has demonstrated clinical benefit for patients with HER2+ metastatic breast cancer, however its clinical activity is limited by inherent or acquired drug resistance. The molecular mechanisms that drive clinical resistance to T-DM1, especially in HER2+ tumors, are not well understood. We used HER2+ cell lines to develop models of T-DM1 resistance utilizing a cyclical dosing schema in which cells received T-DM1 in an "on-off" routine until a T-DM1 resistant population was generated...
October 20, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29054983/alk-inhibitor-response-in-melanomas-expressing-eml4-alk-fusions-and-alternate-alk-isoforms
#18
Kasey L Couts, Judson Bemis, Jacqueline A Turner, Stacey M Bagby, Danielle Murphy, Jason Christiansen, Jennifer D Hintzsche, Anh Le, Todd M Pitts, Keith Wells, Allison Applegate, Carol Amato, Pratik Multani, Edna Chow-Maneval, John J Tentler, Yiqun G Shellman, Matthew J Rioth, Aik-Choon Tan, Rene Gonzalez, Theresa Medina, Robert C Doebele, William A Robinson
Oncogenic ALK fusions occur in several types of cancer and can be effectively treated with ALK inhibitors; however, ALK fusions and treatment response have not been characterized in malignant melanomas. Recently, a novel isoform of ALK (ALKATI) was reported in 11% of melanomas but the response of melanomas expressing ALKATI to ALK inhibition has not been well characterized. We analyzed 45 melanoma patient-derived xenograft models for ALK mRNA and protein expression. ALK expression was identified in 11 out of 45 (24...
October 20, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29054977/synthesis-and-degradation-of-camp-in-giardia-lamblia-possible-role-and-characterization-of-a-nucleotidyl-cyclase-with-a-single-cyclase-homology-domain
#19
Vanina Saraullo, Nicolas Di Siervi, Belen Jerez, Carlos Davio, Adolfo Zurita
Despite its importance in the regulation of growth and differentiation processes of a variety of organisms, the mechanism of synthesis and degradation of cAMP has not yet been described in Giardia lamblia In this work, we measured significant quantities of cAMP in trophozoites of G. lamblia incubated in vitro and later detected how it increases during the first hours of encystation, and how it then returns to basal levels at 24 h. Through an analysis of the genome of G. lamblia , we found sequences of three putative enzymes -one phosphodiesterase (gPDE) and two nucleotydyl cyclases (gNC1 and gNC2)- that should be responsible for the regulation of cAMP in G...
October 20, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29054976/regulation-of-insulin-like-growth-factor-receptors-by-ubiquilin1
#20
Zimple Kurlawala, Rain Dunaway, Parag P Shah, Julie A Gosney, Leah J Siskind, Brian P Ceresa, Levi J Beverly
Insulin-like growth factor-1 receptor (IGF1R) is a receptor tyrosine kinase that mediates growth, proliferation and survival. Dysregulation of IGF pathway contributes to initiation, progression and metastasis of cancer and is also involved in diseases of glucose metabolism, such as Diabetes. We have identified Ubiquilin1 (UBQLN1) as a novel interaction partner of IGF1R, IGF2R and Insulin Receptor. UBQLN family of proteins have been studied primarily in the context of protein quality control and in the field of neurodegenerative disorders...
October 20, 2017: Biochemical Journal
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