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https://www.readbyqxmd.com/read/28449478/protective-effect-of-propofol-preconditioning-on-ischemia-reperfusion-injury-in-human-hepatocyte
#1
Yuzhu Zhang, Zhenzhen Chen, Nianhai Feng, Junxia Tang, Xingbo Zhao, Chengxiao Liu, Hongyu Xu, Mengyuan Zhang
BACKGROUND: Blood reperfusion after ischemia is the main measure to restore cell function. This study was aimed to explore the effect of propofol on rat and cell models of liver ischemia-reperfusion (I/R) injury, and to investigate its possible mechanism. METHODS: Wistar rats were divided into four groups: control group, sham group, I/R group, and propofol group. Human hepatocyte HL7702 was divided into six groups: control group, I/R group and propofol (5, 10, 20 and 40 µmol/L) groups...
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28449399/comparative-transcriptomic-analysis-of-bombyx-mori-fat-body-tissue-following-dietary-restriction
#2
Ye Pan, Peng Lü, Qinyun Wang, Feifei Zhu, Chengjun Li, Yuanqing He, Keping Chen
Dietary restriction (DR) refers to a reduction in food intake to induce undernutrition but not malnutrition, which extends the lifespan of multiple species. Although there are invertebrate aging models, such as the Caenorhabditis elegans and Drosophila melanogaster, aging studies in Lepidoptera are few in number and the underlying life-extending molecular mechanisms are not clear. Research on a broader range of animals is necessary to support generalizations on mechanisms of aging and rates of aging. The aim of this study was to further investigate genes and pathways associated with DR in Bombyx mori...
May 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28449397/differential-proteome-profiling-in-the-hippocampus-of-amnesic-mice
#3
Meghraj Singh Baghel, Mahendra Kumar Thakur
Amnesia or memory loss is associated with brain aging and several neurodegenerative pathologies including Alzheimer's disease (AD). This can be induced by a cholinergic antagonist scopolamine, but the underlying molecular mechanism is poorly understood. The present study of proteome profiling in the hippocampus could provide conceptual insights into the molecular mechanisms involved in amnesia. To reveal this, mice were administered scopolamine to induce amnesia and memory impairment was validated by novel object recognition test...
April 27, 2017: Hippocampus
https://www.readbyqxmd.com/read/28449327/skeletal-muscle-il-15-il-15r%C3%AE-and-myofibrillar-protein-synthesis-after-resistance-exercise
#4
Alberto Pérez-López, James McKendry, Marcos Martin-Rincon, David Morales-Alamo, Bárbara Pérez-Köhler, David Valadés, Julia Buján, Jose A L Calbet, Leigh Breen
In vitro and in vivo studies described the myokine IL-15 and its receptor IL-15Rα as anabolic/anti-atrophy agents, however the protein expression of IL-15Rα has not been measured in human skeletal muscle and data regarding IL-15 expression remain inconclusive. The purpose of the study was to determine serum and skeletal muscle IL-15 and IL-15Rα responses to resistance exercise session and to analyse their association with myofibrillar protein synthesis (MPS). Fourteen participants performed a bilateral leg resistance exercise composed of 4 sets of leg press and 4 sets of knee extension at 75% 1RM to task failure...
April 27, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28449248/betanodavirus-dissection-of-the-viral-life-cycle
#5
REVIEW
C-F Low, B Syarul Nataqain, H-Y Chee, M Z H Rozaini, M Najiah
Progressive research has been recently made in dissecting the molecular biology of Betanodavirus life cycle, the causative pathogen of viral encephalopathy and retinopathy in economic important marine fish species. Establishment of betanodavirus infectious clone allows the manipulation of virus genome for functional genomic study, which elucidates the biological event of the viral life cycle at molecular level. The betanodavirus strategizes its replication by expressing anti-apoptosis/antinecrotic proteins to maintain the cell viability during early infection...
April 27, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28449239/the-role-of-the-nuclear-envelope-protein-man1-in-mesenchymal-stem-cell-differentiation
#6
Sandra Bermeo, Ahmed Al-Saedi, Moustapha Kassem, Christopher Vidal, Gustavo Duque
Mutations in MAN1, a protein of the nuclear envelope, cause bone phenotypes characterized by hyperostosis. The mechanism of this pro-osteogenic phenotype remains unknown. We increased and decreased MAN1 expression in mesenchymal stem cells (MSC) upon which standard osteogenic and adipogenic differentiation were performed. MAN1 knockdown increased osteogenesis and mineralization. In contrast, osteogenesis remained stable upon MAN1 overexpression. Regarding a mechanism, we found that low levels of MAN1 facilitated the nuclear accumulation of regulatory smads and smads-related complexes, with a concurrently high expression of nuclear β-Catenin...
April 27, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28449222/tspan33-is-expressed-in-transitional-and-memory-b-cells-but-is-not-responsible-for-high-adam10-expression
#7
César Augusto Pérez-Martínez, José Luis Maravillas-Montero, Iván Meza-Herrera, Felipe Vences-Catalán, Albert Zlotnik, Leopoldo Santos-Argumedo
Tetraspanins are a family of transmembrane proteins that form membrane microdomains. They play important roles in migration, adhesion, and other cellular processes. TspanC8, a subfamily of tetraspanins, were found to associate and promote ADAM10 trafficking and cell surface localization. One of its members, Tspan33, is expressed in activated B cells. Using RT-PCR and flow cytometry, we analyzed the pattern of expression of Tspan33 in B cells from healthy donors. We found Tspan33 expression in early and late stages of B cell development...
April 27, 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/28449207/targeting-anti-apoptotic-bcl2-family-proteins-in-haematological-malignancies-from-pathogenesis-to-treatment
#8
REVIEW
Meike Vogler, Harriet S Walter, Martin J S Dyer
The B-cell lymphoma 2 (BCL2) family of proteins comprise key regulators of apoptosis and are implicated in the pathogenesis of many malignancies, including lymphomas and leukaemias. Targeting of BCL2 proteins can be directly toxic to tumour cells or render them more sensitive to chemotherapy. Inhibition of the anti-apoptotic functions of BCL2 proteins using structure-based design to produce specific inhibitors of protein-protein interactions has been achieved for BCL2, MCL1 and BCL-XL (also termed BCL2L1), providing an armamentarium of new targeted therapies called BH3-mimetics...
April 27, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28449155/mitochondrial-dysfunction-in-diabetic-cardiomyopathy-effect-of-mesenchymal-stem-cell-with-ppar-%C3%AE-agonist-or-exendin-4
#9
Mohamed Abd Elaziz Wassef, Ola M Tork, Laila A Rashed, Walaa Ibrahim, Heba Morsi, Dina Mohamed Mekawy Rabie
Therapy targeting mitochondria may provide novel ways to treat diabetes and its complications. Bone marrow-derived mesenchymal stem cells (MSCs), the peroxisome proliferator-activated receptor gamma (PPAR-γ) agonists and exendin-4; an analog of glucagon-like peptide-1 have shown cardioprotective properties in many cardiac injury models. So, we evaluated their effects in diabetic cardiomyopathy (DCM) in relation to mitochondrial dysfunction. This work included seven groups of adult male albino rats: the control group, the non-treated diabetic group, and the treated diabetic groups: one group was treated with MSCs only, the second with pioglitazone only, the third with MSCs and pioglitazone, the forth with exendin-4 only and the fifth with MSCs and exendin-4...
April 27, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28449134/characterizing-exogenous-mrna-delivery-trafficking-cytoplasmic-release-and-rna-protein-correlations-at-the-level-of-single-cells
#10
Jonathan L Kirschman, Sushma Bhosle, Daryll Vanover, Emmeline L Blanchard, Kristin H Loomis, Chiara Zurla, Kathryn Murray, Blaine C Lam, Philip J Santangelo
The use of synthetic messenger ribonucleic acid (mRNA) to express specific proteins is a highly promising therapeutic and vaccine approach that avoids many safety issues associated with viral or DNA-based systems. However, in order to optimize mRNA designs and delivery, technology advancements are required to study fundamental mechanisms of mRNA uptake and localization at the single-cell and tissue level. Here, we present a single RNA sensitive fluorescent labeling method which allows us to label and visualize synthetic mRNA without significantly affecting function...
April 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28449094/hepatic-tm6sf2-overexpression-affects-cellular-apob-trafficking-plasma-lipid-levels-hepatic-steatosis-and-atherosclerosis
#11
Nicole Ehrhardt, Michael E Doche, Shuang Chen, Hui Z Mao, Meghan T Walsh, Candy Bedoya, Maha Guindi, Weidong Xiong, Joseph Ignatius Irudayam, Jahangir Iqbal, Sebastien Fuchs, Samuel W French, M Mahmood Hussain, Moshe Arditi, Vaithilingaraja Arumugaswami, Miklós Péterfy
The human TM6SF2 gene has been implicated in plasma lipoprotein metabolism, alcoholic and non-alcoholic fatty liver disease and myocardial infarction in multiple genome-wide association studies. To investigate the role of Tm6sf2 in metabolic homeostasis, we generated mice with elevated expression using adeno-associated virus (AAV)-mediated gene delivery. Hepatic overexpression of mouse Tm6sf2 resulted in phenotypes previously observed in Tm6sf2-deficient mice including reduced plasma lipid levels, diminished hepatic TG secretion and increased hepatosteatosis...
April 25, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28449083/proteomic-response-to-linoleic-acid-hydroperoxide-in-saccharomyces-cerevisiae
#12
Patrick J O'Doherty, Alamgir Khan, Adam J Johnson, Peter J Rogers, Trevor D Bailey, Ming J Wu
Yeast AP-1 transcription factor (Yap1p) and the enigmatic oxidoreductases Oye2p and Oye3p are involved in counteracting lipid oxidants and their unsaturated breakdown products. In order to uncover the response to linoleic acid hydroperoxide (LoaOOH) and the roles of Oye2p, Oye3p, and Yap1p, we carried out proteomic analysis of the homozygous deletion mutants oye3Δ, oye2Δ, and yap1Δ alongside the diploid parent strain BY4743. The findings demonstrate that deletion of YAP1 narrowed the response to LoaOOH, as the number of proteins differentially expressed in yap1Δ was 70% of that observed in BY4743...
April 25, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28449074/fireprot-web-server-for-automated-design-of-thermostable-proteins
#13
Milos Musil, Jan Stourac, Jaroslav Bendl, Jan Brezovsky, Zbynek Prokop, Jaroslav Zendulka, Tomas Martinek, David Bednar, Jiri Damborsky
There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiple-point thermostable mutant proteins that combines structural and evolutionary information in its calculation core...
April 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28449068/development-of-a-novel-human-recellularized-endometrium-that-responds-to-a-28-day-hormone-treatment
#14
Susan A Olalekan, Joanna E Burdette, Spiro Getsios, Teresa K Woodruff, J Julie Kim
Three dimensional (3D) in vitro models have been established to study the physiology and pathophysiology of the endometrium. With emerging evidence that the native extracellular matrix (ECM) provides appropriate cues and growth factors essential for tissue homeostasis, we describe, a novel 3D endometrium in vitro model developed from decellularized human endometrial tissue repopulated with primary endometrial cells. Analysis of the decellularized endometrium using mass spectrometry analysis revealed an enrichment of cell adhesion molecules, cytoskeletal proteins and ECM proteins such as Collagen IV and Laminin...
April 25, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28449065/a-homozygous-missense-mutation-in-eral1-encoding-a-mitochondrial-rrna-chaperone-causes-perrault-syndrome
#15
Iliana A Chatzispyrou, Marielle Alders, Sergio Guerrero-Castillo, Ruben Zapata Perez, Martin A Haagmans, Laurent Mouchiroud, Janet Koster, Rob Ofman, Frank Baas, Hans R Waterham, Johannes N Spelbrink, Johan Auwerx, Marcel M Mannens, Riekelt H Houtkooper, Astrid S Plomp
Perrault syndrome (PS) is a rare recessive disorder characterized by ovarian dysgenesis and sensorineural deafness. It is clinically and genetically heterogeneous, and previously mutations have been described in different genes, mostly related to mitochondrial proteostasis. We diagnosed three unrelated females with PS and set out to identify the underlying genetic cause using exome sequencing. We excluded mutations in the known PS genes, but identified a single homozygous mutation in the ERAL1 gene (c.707A>T; p...
April 25, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28449056/plant-hexokinases-are-multifaceted-proteins
#16
Giovanna P Aguilera-Alvarado, Sobeida Sánchez-Nieto
Sugars are the main carbon and energy source in cells, but they can also act as signaling molecules that affect the whole plant life cycle. Certain tissues can produce sugars and supply them to others, and this plant tissue heterogeneity makes sugar signaling a highly complex process that requires elements capable of perceiving changes in sugar concentrations among different tissues, cell compartments and developmental stages. In plants, the regulatory effects of Glucose (Glc) have been the most studied to date...
April 25, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28449039/polyphyllin-i-induces-g2-m-phase-arrest-and-apoptosis-in-u251-human-glioma-cells-via-mitochondrial-dysfunction-and-the-jnk-signaling-pathway
#17
Jiaxin Liu, Yueting Zhang, Li Chen, Fei Yu, Xiaojin Li, Dan Tao, Jianhua Zhao, Shuai Zhou
Glioblastoma is the most aggressive brain tumor, and its prognosis remains poor. Therefore, novel therapeutic strategies are needed for glioma therapy. Polyphyllin I (PPI), a bioactive constituent extracted from Paris polyphylla, was reported to have anti-tumor activity. However, the detailed mechanism for this activity remains unclear. Here, we investigated the inhibitory effects of PPI on glioma cells and its mechanisms in vitro. U251 cells were treated with various concentrations of PPI (2-9 μM) for 24 to 72 h...
April 26, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28449035/identification-and-expression-profiling-of-odorant-binding-proteins-and-chemosensory-proteins-of-daktulosphaira-vitifoliae-hemiptera-phylloxeridae
#18
Jing-Jing Zhao, Yue Zhang, Dong-Sheng Fan, Ji-Nian Feng
In insects, odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are primary peripheral olfactory proteins playing critical roles in odorant detection. In this study, we present the first identification of OBPs and CSPs from the transcriptome of grape phylloxera Daktulosphaira vitifoliae Fitch, an important pest that damages both roots and leaves of grapes. The OBPs contained six conserved cysteine residues and the CSPs contained four conserved cysteine residues in this insect. Phylogenetic analysis showed that most of the olfactory proteins were closely related to OBPs and CSPs from other aphids...
April 25, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28449024/secretagogin-is-expressed-by-developing-neocortical-gabaergic-neurons-in-humans-but-not-mice-and-increases-neurite-arbor-size-and-complexity
#19
Chandrasekhar S Raju, Julien Spatazza, Amelia Stanco, Phillip Larimer, Shawn F Sorrells, Kevin W Kelley, Cory R Nicholas, Mercedes F Paredes, Jan H Lui, Andrea R Hasenstaub, Arnold R Kriegstein, Arturo Alvarez-Buylla, John L Rubenstein, Michael C Oldham
The neocortex of primates, including humans, contains more abundant and diverse inhibitory neurons compared with rodents, but the molecular foundations of these observations are unknown. Through integrative gene coexpression analysis, we determined a consensus transcriptional profile of GABAergic neurons in mid-gestation human neocortex. By comparing this profile to genes expressed in GABAergic neurons purified from neonatal mouse neocortex, we identified conserved and distinct aspects of gene expression in these cells between the species...
April 26, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28449010/excess-of-a-rassf1-targeting-microrna-mir-193a-3p-perturbs-cell-division-fidelity
#20
Sofia Pruikkonen, Marko J Kallio
BACKGROUND: Several microRNA (miRNA) molecules have emerged as important post-transcriptional regulators of tumour suppressor and oncogene expression. Ras association domain family member 1 (RASSF1) is a critical tumour suppressor that controls multiple aspects of cell proliferation such as cell cycle, cell division and apoptosis. The expression of RASSF1 is lost in a variety of cancers due to the promoter hypermethylation. METHODS: miR-193a-3p was identified as a RASSF1-targeting miRNA by a dual screening approach...
April 27, 2017: British Journal of Cancer
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