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https://www.readbyqxmd.com/read/29785754/expression-of-zo-1-and-claudin-1-in-a-3d-epidermal-equivalent-using-canine-progenitor-epidermal-keratinocytes
#1
Keiji Teramoto, Ryota Asahina, Hidetaka Nishida, Hiroaki Kamishina, Sadatoshi Maeda
BACKGROUND: Previous studies indicate that tight junctions are involved in the pathogenesis of canine atopic dermatitis (cAD). An in vitro skin model is needed to elucidate the specific role of tight junctions in cAD. A 3D epidermal equivalent model using canine progenitor epidermal keratinocytes (CPEK) has been established; the expression of tight junctions within this model is uncharacterized. HYPOTHESIS/OBJECTIVES: To investigate the expression of tight junctions in the 3D epidermal equivalent...
May 21, 2018: Veterinary Dermatology
https://www.readbyqxmd.com/read/29764717/mitochondrial-damage-and-cytoskeleton-reorganization-in-human-dermal-fibroblasts-exposed-to-artificial-visible-light-similar-to-screen-emitted-light
#2
Adeline Rascalou, Jérôme Lamartine, Pauline Poydenot, Frédéric Demarne, Nicolas Bechetoille
BACKGROUND: Artificial visible light is everywhere in modern life. Social communication confronts us with screens of all kinds, and their use is on the rise. We are therefore increasingly exposed to artificial visible light, the effects of which on skin are poorly known. OBJECTIVE: The purpose of this study was to model the artificial visible light emitted by electronic devices and assess its effect on normal human fibroblasts. METHODS: The spectral irradiance emitted by electronic devices was optically measured and equipment was developed to accurately reproduce such artificial visible light...
May 5, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29763974/engineering-a-human-skin-equivalent-to-study-dermis-remodeling-and-epidermis-senescence-in-vitro-after-uva-exposure
#3
Costantino Casale, Giorgia Imparato, Francesco Urciuolo, Francesca Rescigno, Sara Scamardella, Maria Escolino, Paolo A Netti
UVA exposure strongly affects the ageing of human skin by modifying both epidermis and dermis and their cross talk as well. The possibility to get a deep understanding in vitro of such crucial mechanism would have a huge impact in the development of anti-ageing compounds. Here we present a full thickness model of human skin equivalent formed by a millimeter-sized dermis completely composed of fibroblasts embedded in their own extra-cellular matrix (ECM). We show that such endogenous nature of the dermis compartment allows the replication of the complexity of the mutual interactions occurring between cellular and extracellular components of the skin under UVA exposure: (i) oxidative stress formation in the whole tissue (dermis and epidermis); (ii) senescence of germinative layer of epidermal tissue in terms of p63, ki67 and activated caspase-3 regulation; (iii) modification of the collagenous network architecture in the dermis compartment...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29754454/heparinoid-suppresses-der-p-induced-il-1%C3%AE-production-by-inhibiting-erk-and-p38-mapk-pathways-in-keratinocytes
#4
Ryo Utsunomiya, Xiuju Dai, Masamoto Murakami, Hidenori Okazaki, Teruko Tsuda, Hideki Mori, Ken Shiraishi, Mikiko Tohyama, Koji Sayama
Epidermal keratinocytes initiate skin inflammation by activating immune cells. The skin barrier is disrupted in atopic dermatitis (AD) and epidermal keratinocytes can be exposed to environmental stimuli, such as house dust mite (HDM) allergens. We showed previously that HDM allergens activate the NLRP3 inflammasome of keratinocytes, thereby releasing pro-inflammatory cytokines. Heparinoid is an effective moisturizer for atopic dry skin. However, a recent report showed that heparinoid treatment can improve inflammation of lichen planus...
May 13, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29754201/skin-bioprinting-a-novel-approach-for-creating-artificial-skin-from-synthetic-and-natural-building-blocks
#5
Robin Augustine
Significant progress has been made over the past few decades in the development of in vitro-engineered substitutes that mimic human skin, either as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. Tissue engineering has been developing as a novel strategy by employing the recent advances in various fields such as polymer engineering, bioengineering, stem cell research and nanomedicine. Recently, an advancement of 3D printing technology referred as bioprinting was exploited to make cell loaded scaffolds to produce constructs which are more matching with the native tissue...
May 12, 2018: Progress in Biomaterials
https://www.readbyqxmd.com/read/29753523/testicular-transposition-in-children-undergoing-brachytherapy-for-bladder-and-or-prostate-rhabdomyosarcoma
#6
Guenolee de Lambert, Cyrus Chargari, Véronique Minard-Colin, Christine Haie-Meder, Florent Guérin, Hélène Martelli
BACKGROUND/PURPOSE: Fertility preservation is a major goal in treatment of children with cancer. We describe a new technique of testicular transposition (TT) in patients treated with pulse-dose-rate (PDR) brachytherapy as part of the multimodal conservative treatment of bladder neck and/or prostate rhabdomyosarcoma (BP RMS). METHODS: Medical records of consecutive patients treated between September 2016 and August 2017 were studied. These patients underwent a TT performed during BP RMS surgery by the same suprapubic incision...
April 13, 2018: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29753234/effect-of-short-term-hazelnut-consumption-on-dna-damage-and-oxidized-ldl-in-children-and-adolescents-with-primary-hyperlipidemia-a-randomized-controlled-trial
#7
Federica Guaraldi, Valeria Deon, Cristian Del Bo', Stefano Vendrame, Marisa Porrini, Patrizia Riso, Ornella Guardamagna
Children with primary hyperlipidemia are prone to develop premature atherosclerosis, possibly associated with increased oxidative stress. Nutritional therapy is the primary strategy in the treatment of hyperlipidemia and associated conditions. Dietary interventions with bioactive-rich foods, such as nuts, may contribute to the modulation of both lipid profile and the oxidative/antioxidant status. Our study aimed to assess the impact of a dietary intervention with hazelnuts on selected oxidative stress markers in children and adolescents with primary hyperlipidemia...
March 20, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29744907/depigmentation-efficacy-of-galacturonic-acid-through-tyrosinase-regulation-in-b16-murine-melanoma-cells-and-a-three-dimensional-human-skin-equivalent
#8
C S Lee, H S Baek, I-H Bae, S J Choi, Y J Kim, J H Lee, J W Kim
Sugar is a well-known cosmetic ingredient for moisturizing skin with minimal side-effects. Several reports have demonstrated an antimelanogenic effect of sugar in melanocytes. We evaluated the whitening efficacy of galacturonic acid (GA), the main component of pectin, as an anti-melanogenic agent. GA significantly suppressed melanin synthesis and secretion in a concentration-dependent manner in α-melanocyte stimulating hormone-treated B16 melanoma cells, and inhibited tyrosinase activity and expression at a dose of 10 mmol/L...
May 9, 2018: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/29742173/photodynamic-diagnosis-with-methyl-5-aminolevulinate-in-squamous-intraepithelial-lesions-of-the-vulva-experimental-research
#9
Lea Leufflen, Aurelie Francois, Julia Salleron, Catherine Barlier, Gilles Dolivet, Frederic Marchal, Lina Bezdetnaya
The incidence of the High-grade Squamous Intraepithelial Lesion of the vulva, formerly vulvar intra-epithelial neoplasia is progressively increasing. Today, an early detection and a precise localization of vulvar lesions are still problematic issues, due to the lack of accuracy of the available diagnostic tool. A new approach is the photodynamic diagnosis based on the fluorescence detection of protoporphyrin IX (PpIX) in cancer cells after topical application of a cream of methyl amino-levulinic acid. This study aimed to evaluate the effectiveness of photodiagnosis in order to discriminate the intensity of PpIX fluorescence between vulvar tumor and healthy skin...
2018: PloS One
https://www.readbyqxmd.com/read/29735881/-the-effect-of-pain-relieving-intervention-during-infiltration-among-gamma-knife-surgery-patients-for-stereotactic-frame-fixation
#10
Young Jun Jang, Hyeon Ok Kim
PURPOSE: This study aimed to compare the effects of three interventions on pain, blood pressure, and pulse rate during infiltration anesthesia in patients about to undergo gamma knife surgeries. METHODS: The three interventions employed in a university-affiliated Hospital in J City, South Korea were as follows: EMLA cream plus Vapocoolant spray (Vapocoolant, n=30), EMLA cream plus 10.0% Lidocaine spray (Lidocaine, n=30), and EMLA cream only (EMLA, n=30). The equivalent control-group pre test - post test study design was used...
April 2018: Journal of Korean Academy of Nursing
https://www.readbyqxmd.com/read/29735562/impact-of-clofazimine-dosing-on-treatment-shortening-of-the-first-line-regimen-in-a-mouse-model-of-tuberculosis
#11
Nicole C Ammerman, Rosemary V Swanson, Elaine M Bautista, Deepak V Almeida, Vikram Saini, Till F Omansen, Haidan Guo, Yong Seok Chang, Si-Yang Li, Asa Tapley, Rokeya Tasneen, Sandeep Tyagi, Fabrice Betoudji, Chivonne Moodley, Bongani Ngcobo, Logan Pillay, Linda A Bester, Sanil D Singh, Richard E Chaisson, Eric Nuermberger, Jacques H Grosset
The anti-leprosy drug clofazimine was recently repurposed as part of a newly endorsed short-course regimen for multidrug-resistant tuberculosis. It also enables significant treatment-shortening when added to the first-line regimen for drug-susceptible tuberculosis in a mouse model. However, clofazimine causes dose- and duration-dependent skin discoloration in patients, and the optimal clofazimine dosing strategy in the context of the first-line regimen is unknown. We utilized a well-established mouse model to systematically address the impact of duration, dose, and companion drugs on the treatment-shortening activity of clofazimine in the first-line regimen...
May 7, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29732336/assessment-of-neutron-contamination-originating-from-the-presence-of-wedge-and-block-in-photon-beam-radiotherapy
#12
M T Bahreyni Toossi, B Khajetash, M Ghorbani
Background: One of the main causes of induction of secondary cancer in radiation therapy is neutron contamination received by patients during treatment. Objective: In the present study the impact of wedge and block on neutron contamination production is investigated. The evaluations are conducted for a 15 MV Siemens Primus linear accelerator. Methods: Simulations were performed using MCNPX Monte Carlo code. 30˚, 45˚ and 60˚ wedges and a cerrobend block with dimensions of 1...
March 2018: Journal of Biomedical Physics & Engineering
https://www.readbyqxmd.com/read/29730334/fractional-sunburn-threshold-uvr-doses-generate-equivalent-vitamin-d-and-dna-damage-in-skin-types-i-vi-but-with-epidermal-dna-damage-gradient-correlated-to-skin-darkness
#13
Barbara B Shih, Mark D Farrar, Marcus S Cooke, Joanne Osman, Abigail K Langton, Richard Kift, Ann R Webb, Jacqueline L Berry, Rachel E B Watson, Andy Vail, Frank R de Gruijl, Lesley E Rhodes
Public health guidance recommends limiting sun-exposure to sub-sunburn levels, but it's unknown whether these can gain vitamin D (for musculoskeletal health) whilst avoiding epidermal DNA damage (initiates skin cancer). Well-characterised healthy humans of all skin types (I-VI; lightest to darkest skin) were exposed to a low dose-series of solar simulated UVR of 20-80% their individual sunburn threshold dose (minimal erythemal dose, MED). Significant UVR dose-responses were seen for serum 25(OH)D and whole epidermal CPD, with as little as 0...
May 3, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29730222/accuracy-of-qpcr-for-quantifying-leishmania-kdna-in-different-skin-layers-of-patients-with-american-tegumentary-leishmaniasis
#14
Lais Sevilha-Santos, de Castro Moreira Dos Santos Júnior Agenor, Silva Viviane Medeiros, Bergmann Jaqueline Osiro, da Silva Eduardo Freitas, Segato Larissa Freire, Arabi Arthur Youssif Mota, de Paula Natália Aparecida, Ribeiro Sampaio Raimunda Nonata, Dolabela de Lima Beatriz, Gomes Ciro Martins
OBJECTIVES: Superficial swab sampling of American tegumentary leishmaniasis (ATL) lesions shows higher amounts of Leishmania than those from biopsy. Subcutaneous involvement is also important in ATL, but parasite quantification according to lesion depth has not been evaluated. We aim to present the best depth at which sampling should be performed for molecular exams of ATL. METHODS: Patients with a clinical presentation compatible with ATL were allocated to ATL and control groups...
May 3, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29725298/3-bromopyruvate-as-an-alternative-option-for-the-treatment-of-protothecosis
#15
Tomasz Jagielski, Katarzyna Niedźwiecka, Katarzyna Roeske, Mariusz Dyląg
Protothecosis is an unusual infection of both humans and animals caused by opportunistically pathogenic microalgae of the genus Prototheca . Until now, no standardized treatment protocols exist for the protothecal disease, boosted by a remarkable resistance of Prototheca spp. to a wide array of antimicrobial agents currently available in clinical use. Consequently, there is an urgent need for new effective drugs against Prototheca algae. In this study, the anti- Prototheca activity of 3-bromopyruvate (3BP), either alone or in combination with amphotericin B (AMB) was assessed in vitro , as well as the cytotoxicity of 3BP toward the bovine mammary epithelial cells and murine skin fibroblasts...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29720680/prediction-of-steam-burns-severity-using-raman-spectroscopy-on-ex-vivo-porcine-skin
#16
Lina Zhai, Christian Adlhart, Fabrizio Spano, Riccardo Innocenti Malini, Agnieszka K Piątek, Jun Li, René M Rossi
Skin burns due to accidental exposure to hot steam have often been reported to be more severe than the ones occurring from dry heat. While skin burns due to flames or radiant heat have been thoroughly characterized, the mechanisms leading to steam burns are not well understood and a conundrum still exists: can second degree burns occur without destruction of the epidermis, i.e. even before first degree burns are detected? Skin permeability is dependent both on temperature and on the kinetic energy of incoming water molecules...
May 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29713425/follow-up-of-mri-bone-marrow-edema-in-the-treated-diabetic-charcot-foot-a-review-of-patient-charts
#17
Ernst-A Chantelau, Sofia Antoniou, Brigitte Zweck, Patrick Haage
Background : Ill-defined areas of water-like signal on bone magnetic resonance imaging (MRI), characterized as bone marrow edema or edema-equivalent signal-changes (EESC), is a hallmark of active-stage pedal neuro-osteoarthropathy (Charcot foot) in painless diabetic neuropathy, and is accompanied by local soft-tissue edema and hyperthermia. The longitudinal effects on EESC of treating the foot in a walking cast were elucidated by reviewing consecutive cases of a diabetic foot clinic. Study design : Retrospective observational study, chart review Material and methods : Cases with active-stage Charcot foot were considered, in whom written reports on baseline and follow-up MRI studies were available for assessment...
2018: Diabetic Foot & Ankle
https://www.readbyqxmd.com/read/29709325/3d-biomaterial-matrix-to-support-long-term-full-thickness-immuno-competent-human-skin-equivalents-with-nervous-system-components
#18
Sarah E Lightfoot Vidal, Kasey A Tamamoto, Hanh Nguyen, Rosalyn D Abbott, Dana M Cairns, David L Kaplan
Current commercially available human skin equivalents (HSEs) are used for relatively short term studies (∼1 week) due in part to the time-dependent contraction of the collagen gel-based matrix and the limited cell types and skin tissue components utilized. In contrast, here we describe a new matrix consisting of a silk-collagen composite system that provides long term, stable cultivation with reduced contraction and degradation over time. This matrix supports full thickness skin equivalents which include nerves...
April 24, 2018: Biomaterials
https://www.readbyqxmd.com/read/29708853/a-method-to-reduce-heat-strain-while-clad-in-encapsulating-outerwear
#19
Dennis Grahn, Megha Makam, H Craig Heller
The use of personal protective equipment (PPE) increases the risk of heat related maladies. A means to enhance heat dissipation capacity of individuals clad in PPE would be of benefit. The glabrous skin regions of the hands, face, and feet are portals for direct heat transfer between the body core and the external environment. The effects of PPE outerwear and palmar glabrous skin cooling on heat storage were assessed. Subjects engaged in fixed load treadmill exercise in a thermoneutral environment (Ta = 20 - 24ºC) or rested in a hot environment (45 ± 0...
April 30, 2018: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/29697862/progress-in-melanoma-modeling-in-vitro
#20
REVIEW
Alessandra Marconi, Marika Quadri, Annalisa Saltari, Carlo Pincelli
Melanoma is one of the most studied neoplasia, although laboratory techniques used for investigating this tumor are not fully reliable. Animal models may not predict the human response due to differences in skin physiology and immunity. In addition, international guidelines recommend to develop processes that contribute to the reduction, refinement and replacement of animals for experiments (3Rs). Adherent cell culture has been widely used for the study of melanoma to obtain important information regarding melanoma biology...
April 26, 2018: Experimental Dermatology
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