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https://www.readbyqxmd.com/read/27914266/skin-integrated-with-perfusable-vascular-channels-on-a-chip
#1
Nobuhito Mori, Yuya Morimoto, Shoji Takeuchi
This paper describes a method for fabricating perfusable vascular channels coated with endothelial cells within a cultured skin-equivalent by fixing it to a culture device connected to an external pump and tubes. A histological analysis showed that vascular channels were constructed in the skin-equivalent, which showed a conventional dermal/epidermal morphology, and the endothelial cells formed tight junctions on the vascular channel wall. The barrier function of the skin-equivalent was also confirmed. Cell distribution analysis indicated that the vascular channels supplied nutrition to the skin-equivalent...
November 27, 2016: Biomaterials
https://www.readbyqxmd.com/read/27911032/a-dermal-equivalent-engineered-with-tgf-%C3%AE-3-expressing-bone-marrow-stromal-cells-and-amniotic-membrane-cosmetic-healing-of-full-thickness-skin-wounds-in-rats
#2
Ali Samadikuchaksaraei, Ahmad Mehdipour, Mehryar Habibi Roudkenar, Javad Verdi, Mohammad Taghi Joghataei, Kamran As'adi, Fatemeh Amiri, Mozhgan Dehghan Harati, Mazaher Gholipourmalekabadi, Nushin Karkuki Osguei
Transforming growth factor beta-3 (TGF-β3) has been shown to decrease scar formation after scheduled topical applications to the cutaneous wounds. This study aimed to continuously deliver TGF-β3, during the early phase of wound healing, by engineering a dermal equivalent (DE) using TGF-β3 expressing bone marrow stromal cells (BM-SCs) and human dehydrated amniotic membrane (hDAM). To engineer a DE, rat BM-SCs were seeded on the hDAM and TGF-β3 was transiently transfected into the BM-SCs using a plasmid vector...
December 2016: Artificial Organs
https://www.readbyqxmd.com/read/27904077/synthesis-characterization-and-evaluation-of-thiazolidine-derivatives-of-cysteine-for-suppressing-eumelanin-production
#3
Yusuke Amino, Yoshinobu Takino, Megumi Kaneko, Fumie Ookura, Mai Yamamoto, Tatsuki Kashiwagi, Keiji Iwasaki
In the pathway of melanin biosynthesis, cysteine (Cys) is utilized for the synthesis of pheomelanin. Accordingly, Cys is considered to suppress the formation of brown-black eumelanin. Although attempts have been made to utilize Cys and its derivatives as skin-whitening agents, their instability and odor hinders their application as a cosmetic agent. Herein, N-acetyl-2-methylthiazolidine-2,4-dicarboxylic acid ethyl ester (AcCP2Et) was proposed as a candidate for a stable and prolonged-release derivative of Cys to inhibit dopachrome formation after its degradation in melanocytes...
2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27900769/influence-of-menstrual-phase-and-arid-vs-humid-heat-stress-on-autonomic-and-behavioural-thermoregulation-during-exercise-in-trained-but-unacclimated-women
#4
Tze-Huan Lei, Stephen R Stannard, Blake G Perry, Zachary J Schlader, James D Cotter, Toby Mündel
We studied thermoregulatory responses of ten well-trained (VO2 max, 57 (7) mL min(-1) kg(-1) ) eumenorrheic women exercising in dry and humid heat, across their menstrual cycle. They completed four trials, each of resting and cycling at fixed intensities (125 and 150 W), to assess autonomic regulation, then self-paced intensity (30-min work trial), to assess behavioural regulation. Trials were in early-follicular (EF) and mid-luteal (ML) phases in dry (DRY) and humid (HUM) heat matched for wet bulb globe temperature (WBGT, 27°C)...
November 29, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/27900658/fixed-combination-gels-of-adapalene-and-benzoyl-peroxide-provide-optimal-percutaneous-absorption-compared-to-monad-formulations-of-these-compounds-results-from-two-in-vitro-studies
#5
Hanan Osman-Ponchet, Karine Sevin, Alexandre Gaborit, Nathalie Wagner, Michel Poncet
INTRODUCTION: Adapalene 0.1%/benzoyl peroxide 2.5% (0.1% A/BPO) and adapalene 0.3%/BPO 2.5% (0.3% A/BPO) gels are fixed-combination options for the topical treatment of acne. However, the active compounds of these combinations are also available as monads, to be used in association or as monotherapy. These two in vitro studies determined the effect of different treatment regimens on the percutaneous absorption of adapalene (0.1% and 0.3%) gels and BPO 2.5% gel in ex vivo human skin. METHODS: In vitro percutaneous absorption studies were conducted using full-thickness human skin from six donors...
November 30, 2016: Dermatology and Therapy
https://www.readbyqxmd.com/read/27892606/svep1-plays-a-crucial-role-in-epidermal-differentiation
#6
L Samuelov, Q Li, R Bochner, N Najor, L Albrecht, N Malchin, T Goldsmith, M Grafi-Cohen, D Vodo, G Fainberg, B Meilik, I Goldberg, E Warshauer, T Rogers, S Edie, A Ishida-Yamamoto, L Burzenski, N Erez, S A Murray, A D Irvine, L Shultz, K Green, J Uitto, E Sprecher, O Sarig
SVEP1 is a recently identified multi-domain cell adhesion protein, homologous to the mouse polydom protein, which has been shown to mediate cell-cell adhesion in an integrin dependent-manner in osteogenic cells. In the present study, we characterized SVEP1 function in the epidermis. SVEP1 was found by qRT-PCR to be ubiquitously expressed in human tissues, including the skin. Confocal microscopy revealed that SVEP1 is normally mostly expressed in the cytoplasm of basal and suprabasal epidermal cells. Down-regulation of SVEP1 expression in primary keratinocytes resulted in decreased expression of major epidermal differentiation markers...
November 28, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27877065/poor-adhesion-of-fentanyl-transdermal-patches-may-mimic-end-of-dosage-failure-after-48-hours-and-prompt-early-patch-replacement-in-hospitalized-cancer-pain-patients
#7
Isabelle Arnet, Sabrina Schacher, Eva Balmer, Dieter Koeberle, Kurt E Hersberger
CONTEXT: Renewal of fentanyl transdermal patch (transdermal therapeutic system [TTS]) should occur every 3 days (72 hours) according to manufacturer's guidelines. Some studies mentioned patients reporting end-of-dose failure, and thus, some authors recommend shortening the interval of application to 2 days (48 hours). However, reasons for early replacement are mostly unknown. OBJECTIVES: The objectives of this study were to assess the prevalence of early replacement of fentanyl TTS in a cancer center in Basel, Switzerland, and to assess the reasons for early replacement in stationary patients...
2016: Journal of Pain Research
https://www.readbyqxmd.com/read/27873527/numerical-evaluation-of-scar-after-breast-reconstruction-with-abdominal-advancement-flap
#8
M Kovář, L Čapek, L Vítová, M Molitor
BACKGROUND: The treatment of breast cancer has developed a lot during the last decade, nevertheless it still remains a considerable social and economical problem all over the world. The choice of the surgical procedure depends on a patients protocol and the surgeons preferences. The aim of this study is to evaluate the stress on the scar after breast reconstruction. METHODS: Mathematical modeling of the sutured skin flap used for breast implant placement was divided into the following two steps...
2016: Acta Chirurgiae Plasticae
https://www.readbyqxmd.com/read/27869214/irradiance-dependent-uvb-photocarcinogenesis
#9
Cheng-Che E Lan, Ching-Shuang Wu, Shu-Mei Huang, Chin-Han Wu, Hsiao-Chi Lai, Yu-Ting Peng, Pao-Sheng Hou, Hui-Jun Yang, Gwo-Shing Chen
Ultraviolet B (UVB) radiation from the sun may lead to photocarcinogenesis of the skin. Sunscreens were used to protect the skin by reducing UVB irradiance, but sunscreen use did not reduce sunburn episodes. It was shown that UVB-induced erythema depends on surface exposure but not irradiance of UVB. We previously showed that irradiance plays a critical role in UVB-induced cell differentiation. This study investigated the impact of irradiance on UVB-induced photocarcinogenesis. For hairless mice receiving equivalent exposure of UVB radiation, the low irradiance (LI) UVB treated mice showed more rapid tumor development, larger tumor burden, and more keratinocytes harboring mutant p53 in the epidermis as compared to their high irradiance (HI) UVB treated counterpart...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27868148/the-alarmins-hmbg1and-interleukin-33-downregulate-the-structural-skin-barrier
#10
Uffe Nygaard, Ellen H van den Bogaard, Hanna Niehues, Malene Hvid, Mette Deleuran, Claus Johansen, Christian Vestergaard
The epidermal-derived "alarmins" high-mobility group box 1 (HMGB1) protein and interleukin-33 (IL-33) are upregulated in patients with atopic dermatitis. However, their capacity as pro-inflammatory cytokines and their derived effects on skin barrier regulation are poorly elucidated. We investigated the impact of HMGB1 and IL-33 on gene transcription, protein expression and epidermal differentiation across 3 distinct keratinocyte in vitro models. Primary keratinocytes from healthy donors were used in submerged monolayer cultures, 3D human epidermis equivalents and 3D human skin equivalents...
October 14, 2016: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/27867696/quantification-of-extra-cerebral-and-cerebral-hemoglobin-concentrations-during-physical-exercise-using-time-domain-near-infrared-spectroscopy
#11
Héloïse Auger, Louis Bherer, Étienne Boucher, Richard Hoge, Frédéric Lesage, Mathieu Dehaes
Fitness is known to have beneficial effects on brain anatomy and function. However, the understanding of mechanisms underlying immediate and long-term neurophysiological changes due to exercise is currently incomplete due to the lack of tools to investigate brain function during physical activity. In this study, we used time-domain near infrared spectroscopy (TD-NIRS) to quantify and discriminate extra-cerebral and cerebral hemoglobin concentrations and oxygen saturation (SO2) in young adults at rest and during incremental intensity exercise...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27867271/wound-healing-activity-and-chemical-standardization-of-eugenia-pruniformis-cambess
#12
Ricardo Diego Duarte Galhardo de Albuquerque, Jamila Alessandra Perini, Daniel Escorsim Machado, Thaís Angeli-Gamba, Ricardo Dos Santos Esteves, Marcelo Guerra Santos, Adriana Passos Oliveira, Leandro Rocha
BACKGROUND: Eugenia pruniformis is an endemic species from Brazil. Eugenia genus has flavonoids as one of the remarkable chemical classes which are related to the improvement of the healing process. AIMS: To evaluate of wound healing activity of E. pruniformis leaves and to identify and quantify its main flavonoids compounds. MATERIALS AND METHODS: Wound excision model in rats was used to verify the hydroethanolic and ethyl acetate extracts potential...
October 2016: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/27853671/heparanase-inhibitors-facilitate-the-assembly-of-the-basement-membrane-in-artificial-skin
#13
Makoto Tsunenaga
Recent research suggests that the basement membrane at the dermal-epidermal junction of the skin plays an important role in maintaining a healthy epidermis and dermis, and repeated damage to the skin can destabilize the skin and accelerate the aging process. Skin-equivalent models are suitable for studying the reconstruction of the basement membrane and its contribution to epidermal homeostasis because they lack the basement membrane and show abnormal expression of epidermal differentiation markers. By using these models, it has been shown that reconstruction of the basement membrane is enhanced not only by supplying basement membrane components, but also by inhibiting proteinases such as urokinase and matrix metalloproteinase...
August 2016: Current Tissue Engineering
https://www.readbyqxmd.com/read/27829611/sodium-lauryl-sulfate-stimulates-the-generation-of-reactive-oxygen-species-through-interactions-with-cell-membranes
#14
Taeko Mizutani, Ryota Mori, Misaki Hirayama, Yuki Sagawa, Kenji Shimizu, Yuri Okano, Hitoshi Masaki
Sodium lauryl sulfate (SLS), a representative anionic surfactant, is well-known to induce rough skin following single or multiple topical applications. The mechanism by which SLS induces rough skin is thought to result from the disruption of skin moisture function consisting of NMF and epidermal lipids. However, a recent study demonstrated that topically applied SLS easily penetrates into the living cell layers of the epidermis, which suggests that physiological alterations of keratinocytes might cause the SLS-induced rough skin...
December 1, 2016: Journal of Oleo Science
https://www.readbyqxmd.com/read/27827560/development-of-a-novel-automated-hair-counting-system-for-the-quantitative-evaluation-of-laser-hair-removal
#15
Hyoung-Woo Lim, Minwoo Cho, Dong-Hun Lee, Wooseok Koh, Youdan Kim, Jin Ho Chung, Sungwan Kim
OBJECTIVE: We aimed to develop and validate a novel computer-assisted automated hair counting system for the quantitative evaluation of laser hair removal (LHR). METHODS: We developed a computer-aided image processing system to count hairs on shaved skin and validated its performance through clinical trials. Five volunteers of Fitzpatrick skin type III-IV volunteered and were tested on both thighs. The system automatically detects hair and places a "+" sign on each hair site for every positive detection...
November 3, 2016: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/27824929/tissue-kallikrein-inhibitors-based-on-the-sunflower-trypsin-inhibitor-scaffold-a-potential-therapeutic-intervention-for-skin-diseases
#16
Wenjie Chen, Veronica A Kinsler, Derek Macmillan, Wei-Li Di
Tissue kallikreins (KLKs), in particular KLK5, 7 and 14 are the major serine proteases in the skin responsible for skin shedding and activation of inflammatory cell signaling. In the normal skin, their activities are controlled by an endogenous protein protease inhibitor encoded by the SPINK5 gene. Loss-of-function mutations in SPINK5 leads to enhanced skin kallikrein activities and cause the skin disease Netherton Syndrome (NS). We have been developing inhibitors based on the Sunflower Trypsin Inhibitor 1 (SFTI-1) scaffold, a 14 amino acids head-to-tail bicyclic peptide with a disulfide bond...
2016: PloS One
https://www.readbyqxmd.com/read/27821307/in%C3%A2-vitro-activation-of-the-neuro-transduction-mechanism-in-sensitive-organotypic-human-skin-model
#17
Francesca Martorina, Costantino Casale, Francesco Urciuolo, Paolo A Netti, Giorgia Imparato
Recent advances in tissue engineering have encouraged researchers to endeavor the production of fully functional three-dimensional (3D) thick human tissues in vitro. Here, we report the fabrication of a fully innervated human skin tissue in vitro that recapitulates and replicates skin sensory function. Previous attempts to innervate in vitro 3D skin models did not demonstrate an effective functionality of the nerve network. In our approach, we initially engineer functional human skin tissue based on fibroblast-generated dermis and differentiated epidermis; then, we promote rat dorsal root ganglion (DRG) neurons axon ingrowth in the de-novo developed tissue...
January 2017: Biomaterials
https://www.readbyqxmd.com/read/27816673/comparison-of-balb-c-and-cba-j-mice-for-the-local-lymph-node-assay-using-bromodeoxyuridine-with-flow-cytometry-llna-brdu-fcm
#18
Yong Sun Lee, Jung-Sun Yi, Souk Jin Seo, Joo Hwan Kim, Mi-Sook Jung, Im-Kwon Seo, Ilyoung Ahn, Kyungyuk Ko, Tae Sung Kim, Kyung Min Lim, Soojung Sohn
The local lymph node assay using 5-bromo-2-deoxyuridine (BrdU) with flow cytometry (LLNA: BrdU-FCM) is a modified LLNA that is used to identify skin sensitizers by counting BrdU-incorporated lymph node cells (LNCs) with flow cytometry. Unlike other LLNA methods (OECD TG 429, 442A and 442B) in which the CBA/J mouse strain is used, LLNA: BrdU-FCM was originally designed to be compatible with BALB/c, a mouse strain that is more widely used in many countries. To justify the substitution of CBA/J for BALB/c, the equivalence of the test results between two strains shall be established prior to the official implementation of LLNA: BrdU-FCM...
November 2, 2016: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/27812584/the-nanotipped-hairs-of-gecko-skin-and-biotemplated-replicas-impair-and-or-kill-pathogenic-bacteria-with-high-efficiency
#19
X Li, G S Cheung, G S Watson, J A Watson, S Lin, L Schwarzkopf, D W Green
We show that gecko microspinules (hairs) and their equivalent replicas, bearing nanoscale tips, can kill or impair surface associating oral pathogenic bacteria with high efficiency even after 7 days of repeated attacks. Scanning Electron Microscopy suggests that there is more than one mechanism contributing to cell death which appears to be related to the scaling of the bacteria type with the hair arrays and accessibility to the underlying nano-topography of the hierarchical surfaces.
December 7, 2016: Nanoscale
https://www.readbyqxmd.com/read/27806156/reliability-and-photographic-equivalency-of-the-scar-cosmesis-assessment-and-rating-scar-scale-an-outcome-measure-for-postoperative-scars
#20
Jonathan Kantor
Importance: Until recently, no ideal valid, feasible, and reliable scar scale existed to effectively assess the quality of postoperative linear scars. The Scar Cosmesis Assessment and Rating (SCAR) scale was developed and validated as a tool to assess the quality of postoperative scars in clinical and research settings. Objective: To assess the reliability of using photographs in lieu of live patient scar rating assessments, and to determine the interrater and intrarater reliability of the SCAR scale...
November 2, 2016: JAMA Dermatology
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