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https://www.readbyqxmd.com/read/27898233/hyaluronan-stimulates-chondrogenic-gene-expression-in-human-meniscus-cells
#1
Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshifumi Ozaki
: Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. MATERIALS AND METHODS: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco's modified Eagle's medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2-7 days...
November 29, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27890729/maturation-of-human-embryonic-stem-cell-derived-cardiomyocytes-hesc-cms-in-3d-collagen-matrix-effects-of-niche-cell-supplementation-and-mechanical-stimulation
#2
W Zhang, C W Kong, M H Tong, W H Chooi, N Huang, R A Li, B P Chan
: Cardiomyocytes derived from human embryonic stem cells (hESC-CMs) are regarded as a promising source for regenerative medicine, drug testing and disease modeling. Nevertheless, cardiomyocytes are immature in terms of their contractile structure, metabolism and electrophysiological properties. Here, we fabricate cardiac muscle strips by encapsulating hESC-CMs in collagen-based biomaterials. Supplementation of niche cells at 3% to the number of hESC-CMs enhance the maturation of the hESC-CMs in 3D tissue matrix...
November 24, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27888100/harnessing-wharton-s-jelly-stem-cell-differentiation-into-bone-like-nodule-on-calcium-phosphate-substrate-without-osteoinductive-factors
#3
S Mechiche Alami, H Rammal, C Boulagnon-Rombi, F Velard, F Lazar, R Drevet, D Laurent Maquin, S C Gangloff, J Hemmerlé, J C Voegel, G Francius, P Schaaf, F Boulmedais, H Kerdjoudj
: An important aim of bone regenerative medicine is to design biomaterials with controlled chemical and topographical features to guide stem cell fate towards osteoblasts without addition of specific osteogenic factors. Herein, we find that sprayed bioactive and biocompatible calcium phosphate substrates (CaP) with controlled topography induce, in a well-orchestrated manner, Wharton's jelly stem cells (WJ-SCs) differentiation into osteoblastic lineage without any osteogenic supplements...
November 22, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27882132/silencing-of-wnt5a-prevents-interleukin-1%C3%AE-induced-collagen-type-ii-degradation-in-rat-chondrocytes
#4
Shiping Shi, Zhentao Man, Wei Li, Shui Sun, Wei Zhang
Osteoarthritis (OA) is a joint disease, and few treatments to date have been able to delay OA progression. The degradation of collagen type II (COL2) in the cartilage matrix is an important initiating factor for OA progression; the upregulation of Wnt5a protein activates COL2 degradation. In the present study, small interfering RNA of Wnt-5a was delivered by a lentiviral vector (LV-Wnt5a-RNAi) to silence Wnt-5a mRNA and prevent COL2 degradation. To determine the function of LV-Wnt5a-RNAi, the OA chondrocyte model (OA-like chondrocytes) were constructed using interleukin (IL)-1β...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27877077/amelioration-of-estrogen-deficiency-induced-obesity-by-collagen-hydrolysate
#5
Tsay-I Chiang, I-Chang Chang, Hsueh-Hui Lee, Kuang Hui Hsieh, Yung-Wei Chiu, Te-Jen Lai, Jer-Yuh Liu, Li-Sung Hsu, Shao-Hsuan Kao
Objectives: Menopausal transition with declining estrogen levels significantly affects the physiological properties of women and consequently contributes to a series of medical conditions, including obesity. Obesity is a crucial risk factor associated with cardiovascular diseases, diabetes mellitus, and breast cancer. Increasing dietary protein content improves satiety and energy expenditure. Thus, we hypothesize that supplementing with collagen, a common dietary protein, may alleviate menopause-induced obesity...
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27876676/anatomical-region-dependent-enhancement-of-3-dimensional-chondrogenic-differentiation-of-human-mesenchymal-stem-cells-by-soluble-meniscus-extracellular-matrix
#6
Benjamin B Rothrauff, Kazunori Shimomura, Riccardo Gottardi, Peter G Alexander, Rocky S Tuan
: Extracellular matrix (ECM) derived from decellularized tissues has been found to promote tissue neogenesis, most likely mediated by specific biochemical and physical signaling motifs that promote tissue-specific differentiation of progenitor cells. Decellularized ECM has been suggested to be efficacious for the repair of tissue injuries. However, decellularized meniscus contains a dense collagenous structure, which impedes cell seeding and infiltration and is not readily applicable for meniscus repair...
November 19, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27870875/a-novel-organ-culture-model-to-quantify-collagen-remodeling-in-tree-shrew-sclera
#7
Sarah Baldivia, Alexander Levy, Shylaja Hegde, Stijn J A Aper, Maarten Merkx, Rafael Grytz
Increasing evidence suggests that unknown collagen remodeling mechanisms in the sclera underlie myopia development. We are proposing a novel organ culture system in combination with two-photon fluorescence imaging to quantify collagen remodeling at the tissue- and lamella-level. Tree shrew scleral shells were cultured up to 7 days in serum-free media and cellular viability was investigated under: (i) minimal tissue manipulations; (ii) removal of intraocular tissues; gluing the eye to a washer using (iii) 50 μL and (iv) 200 μL of cyanoacrylate adhesive; (v) supplementing media with Ham's F-12 Nutrient Mixture; and (vi) culturing eyes subjected to 15 mmHg intraocular pressure in our new bioreactor...
2016: PloS One
https://www.readbyqxmd.com/read/27852613/vitamin-c-enriched-gelatin-supplementation-before-intermittent-activity-augments-collagen-synthesis
#8
Gregory Shaw, Ann Lee-Barthel, Megan Lr Ross, Bing Wang, Keith Baar
BACKGROUND: Musculoskeletal injuries are the most common complaint in active populations. More than 50% of all injuries in sports can be classified as sprains, strains, ruptures, or breaks of musculoskeletal tissues. Nutritional and/or exercise interventions that increase collagen synthesis and strengthen these tissues could have an important effect on injury rates. OBJECTIVE: This study was designed to determine whether gelatin supplementation could increase collagen synthesis...
November 16, 2016: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27829314/bone-morphogenetic-protein-2-but-not-mesenchymal-stromal-cells-exert-regenerative-effects-on-canine-and-human-nucleus-pulposus-cells
#9
Frances C Bach, Alberto Miranda-Bedate, Ferdi W M van Heel, Frank M Riemers, Margot C M E Müller, Laura B Creemers, Keita Ito, Karin Benz, Björn P Meij, Marianna A Tryfonidou
Chronic back pain is related to intervertebral disc (IVD) degeneration and dogs are employed as animal models to develop growth factor- and cell-based regenerative treatments. In this respect, the differential effects of transforming growth factor beta-1 (TGF-β1) and bone morphogenetic protein-2 (BMP2) on canine and human chondrocyte-like cells (CLCs) derived from the nucleus pulposus of degenerated IVDs were studied. Human and canine CLCs were cultured in 3D microaggregates in basal culture medium supplemented with/without TGF-β1 (10 ng/mL) or BMP2 (100 or 250 ng/mL)...
December 12, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27828597/nutritional-supplementation-with-arginine-protects-radiation-induced-effects-an-experimental-study
#10
Flavia Cristina Morone Pinto, Pamella Campos-Silva, Diogo Benchimol de Souza, Waldemar Silva Costa, Francisco José Barcellos Sampaio
PURPOSE: To investigate the protective effect of L-arginine on the prostate (nonneoplasic) of rats with radiation-induced injury. METHODS: Twenty-nine Wistar rats, male adult, allocated into three groups: Control group (C) was not exposed to irradiation (n=10); Radiated group (R) had undergone pelvic irradiation (n=10); Supplemented and radiated group (R+S) had undergone pelvic irradiation plus L-arginine supplementation (n=9). The animals were observed for signs of toxicity...
October 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27824284/formation-of-hyaline-cartilage-tissue-by-passaged-human-osteoarthritic-chondrocytes
#11
Vanessa Juliana Bianchi, Joanna F Weber, Stephen D Waldman, David Backstein, Rita Kandel
Objective When serially passaged in standard monolayer culture to expand cell number, articular chondrocytes lose their phenotype. This results in the formation of fibrocartilage when they are used clinically, thus limiting their use for cartilage repair therapies. Identifying a way to redifferentiate these cells in vitro is critical if they are to be used successfully. Transforming growth factor beta (TGFβ) family members are known to be crucial for regulating differentiation of fetal limb mesenchymal cells and mesenchymal stromal cells to chondrocytes...
November 8, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27822012/cardioprotective-activity-of-placental-growth-factor-combined-with-oral-supplementation-of-l-arginine-in-a-rat-model-of-acute-myocardial-infarction
#12
Liyun Luo, Bairong Chen, Yin Huang, Zibin Liang, Songbiao Li, Yuelan Yin, Jian Chen, Wei Wu
OBJECTIVE: Exogenous administration of placental growth factor (PlGF) stimulates angiogenesis and improves ventricular remodeling after acute myocardial infarction (AMI), and supplementation with l-arginine ameliorates endothelial function. The objective of the present study was to compare the cardioprotective effects of combination therapy of PlGF and l-arginine with those of direct administration of PlGF alone in a rat model of AMI. MATERIALS AND METHODS: Fifty male Sprague Dawley rats were randomly divided into five groups: sham group, normal saline group, l-arginine group, PlGF group, and combination group (PlGF + l-arginine)...
2016: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/27821943/green-tea-and-exercise-interventions-as-nondrug-remedies-in-geriatric-patients-with-rheumatoid-arthritis
#13
Ahmad H Alghadir, Sami A Gabr, Einas S Al-Eisa
This study aimed to evaluate the effects of green tea and supervised exercise training interventions on improvement of disease activity and bone metabolism markers in rheumatoid arthritis patients. [Subjects and Methods] One-hundred and twenty subjects who had a mean age of (60.7 ± 2.53 years) and had been diagnosed with rheumatoid arthritis at least ten years previously were randomly included in this study. Patients were treated with infliximab, green tea, or a supervised exercise program for six months...
October 2016: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/27820813/collagen-intrafibrillar-mineralization-as-a-result-of-the-balance-between-osmotic-equilibrium-and-electroneutrality
#14
Li-Na Niu, Sang Eun Jee, Kai Jiao, Lige Tonggu, Mo Li, Liguo Wang, Yao-Dong Yang, Ji-Hong Bian, Lorenzo Breschi, Seung Soon Jang, Ji-Hua Chen, David H Pashley, Franklin R Tay
Mineralization of fibrillar collagen with biomimetic process-directing agents has enabled scientists to gain insight into the potential mechanisms involved in intrafibrillar mineralization. Here, by using polycation- and polyanion-directed intrafibrillar mineralization, we challenge the popular paradigm that electrostatic attraction is solely responsible for polyelectrolyte-directed intrafibrillar mineralization. As there is no difference when a polycationic or a polyanionic electrolyte is used to direct collagen mineralization, we argue that additional types of long-range non-electrostatic interaction are responsible for intrafibrillar mineralization...
November 7, 2016: Nature Materials
https://www.readbyqxmd.com/read/27818718/built-in-microscale-electrostatic-fields-induced-by-anatase-rutile-phase-transition-in-selective-areas-promote-osteogenesis
#15
Chengyun Ning, Peng Yu, Ye Zhu, Mengyu Yao, Xiaojing Zhu, Xiaolan Wang, Zefeng Lin, Weiping Li, Shuangying Wang, Guoxin Tan, Yu Zhang, Yingjun Wang, Chuanbin Mao
Bone has a built-in electric field because of the presence of piezoelectric collagen. To date, only externally applied electric fields have been used to direct cell behavior; however, these fields are not safe or practical for in vivo use. In this work, for the first time, we use a periodic microscale electric field (MEF) built into a titanium implant to induce osteogenesis. Such a MEF is generated by the periodic organization of a junction made of two parallel semiconducting TiO2 zones: anatase and rutile with lower and higher electron densities, respectively...
2016: NPG Asia materials
https://www.readbyqxmd.com/read/27813341/pharmacological-and-biological-therapeutic-strategies-for-osteogenesis-imperfecta
#16
Ronit Marom, Yi-Chien Lee, Ingo Grafe, Brendan Lee
Osteogenesis imperfecta (OI) is a connective tissue disorder characterized by bone fragility, low bone mass, and bone deformities. The majority of cases are caused by autosomal dominant pathogenic variants in the COL1A1 and COL1A2 genes that encode type I collagen, the major component of the bone matrix. The remaining cases are caused by autosomal recessively or dominantly inherited mutations in genes that are involved in the post-translational modification of type I collagen, act as type I collagen chaperones, or are members of the signaling pathways that regulate bone homeostasis...
November 3, 2016: American Journal of Medical Genetics. Part C, Seminars in Medical Genetics
https://www.readbyqxmd.com/read/27813317/bone-forming-cells-with-pronounced-spread-into-the-third-dimension-in-polymer-scaffolds-fabricated-by-two-photon-polymerization
#17
J Heitz, C Plamadeala, M Wiesbauer, P Freudenthaler, R Wollhofen, J Jacak, T A Klar, B Magnus, D Köstner, A Weth, W Baumgartner, R Marksteiner
The main aim of this work was to stimulate bone-forming cells to produce three-dimensional networks of mineralized proteins such as those occurring in bones. This was achieved by a novel approach using a specific type of mesenchymal progenitor cells (i.e., primary fibroblast cells from human hair roots) seeded onto polymer scaffolds. We wrote polymer microstructures with one or more levels of quadratic pores onto a flexible substrate by means of two-photon polymerization using a Ti-sapphire femtosecond laser focused into a liquid acrylate-based resin containing a photoinitiator...
November 3, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27783156/arginase-activity-urea-and-hydroxyproline-concentration-are-reduced-in-keratoconus-keratocytes
#18
Tanja Stachon, Krasimir Kolev, Zsuzsa Flaskó, Berthold Seitz, Achim Langenbucher, Nóra Szentmáry
PURPOSE: Keratoconus (KC) is a disease characterized by thinning and deformation of the cornea, but its etiology remains unknown. Seventy percent of the corneal stroma consists of collagen, which is composed of three intertwined polypeptide chains with glycine-hydroxyproline-proline repeats along their sequence. Arginase is a cytoplasmatic enzyme and catalyzes the conversion of arginine to urea and ornithine, which serves as a precursor for the endogenous synthesis of proline and hydroxyproline...
October 25, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27754256/os-36-07-treatment-with-apelin-13-prevents-pressure-overload-induced-aortic-adventitial-remodeling-and-fibrosis-in-hypertensive-rats-with-tac
#19
Jiu-Chang Zhong, Zhen-Zhou Zhang, Ran Xu, Lai-Jiang Chen, Shu-Jie Guo, Ying-Le Xu, Gavin Oudit, Hai-Yan Jin, Bei Song, Qing Chang, Ping-Jin Gao, Ji-Guang Wang
OBJECTIVE: The Apelin/APJ system has recently been implicated in pathologies of hypertension. However, little was elucidated upon effects of Apelin on vascular adventitial remodeling progression. Here, we examined regulatory roles of Apelin in pressure overload-induced adventitial remodeling and fibrosis in hypertensive rats. DESIGN AND METHOD: The male Sprague-Dawley rats were performed with transverse aortic constriction (TAC). The rats with TAC were randomized to daily deliver either pyroglutamyl Apelin-13 (50 μg/kg) or saline for 4 weeks...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27743017/longitudinal-evaluation-of-bone-mineral-density-and-bone-metabolism-markers-in-patients-with-indolent-systemic-mastocytosis-without-osteoporosis
#20
A Artuso, C Caimmi, G Tripi, O Viapiana, M Bonifacio, L Idolazzi, I Gavioli, D Gatti, R Zanotti, M Rossini
Systemic Mastocytosis has been long identified as a potential cause of osteoporosis; nevertheless, data regarding longitudinal variation of bone mineral density (BMD) in patients with indolent systemic mastocytosis (ISM) are missing . We studied BMD variation at lumbar spine and proximal hip after 30-month (±6 months) follow-up in a large cohort of patients (83) with ISM without osteoporosis, supplementated with vitamin D and/or calcium when needed. We also analyzed the correlation between variation of BMD, basal serum tryptase levels and bone turnover markers (BTM)...
October 14, 2016: Calcified Tissue International
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