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https://www.readbyqxmd.com/read/29790092/effects-of-olive-leaf-powder-supplemented-to-fish-feed-on-muscle-protein-of-red-sea-bream
#1
Muh Ali Arsyad, Takashi Akazawa, Chie Nozaki, Makoto Yoshida, Kenichi Oyama, Tatsuo Mukai, Masahiro Ogawa
Olive leaf is known to have the high polyphenol content of 6-9% in dry weight. We investigated the effects of olive leaf powder (OLP) supplemented to fish feed on muscle protein of red sea bream (Pagrus major). Fish reared with feed containing 8% OLP for 40 days had 1.4 times higher myofibril content and 2.2 times higher acid-soluble collagen content than fish reared with control feed for the same period. On the other hand, sarcoplasmic protein content and collagenase activity of the muscle were almost the same between the control fish and OLP-diet fish...
May 22, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29782187/effects-of-maternal-vitamin-d-supplementation-on-the-maternal-and-infant-epigenome
#2
Cindy M Anderson, Shannon L Gillespie, Doria K Thiele, Jody L Ralph, Joyce E Ohm
INTRODUCTION: Mothers and infants are at high risk for inadequate vitamin D status. Mechanisms by which vitamin D may affect maternal and infant DNA methylation are poorly understood. OBJECTIVE: This study quantified the effects of vitamin D3 supplementation on DNA methylation in pregnant and lactating women and their breastfed infants. MATERIALS AND METHODS: In this randomized controlled pilot study, pregnant women received vitamin D3 400 international units (IU) (n = 6; control) or 3,800 IU (n = 7; intervention) daily from late second trimester through 4-6 weeks postpartum...
May 21, 2018: Breastfeeding Medicine: the Official Journal of the Academy of Breastfeeding Medicine
https://www.readbyqxmd.com/read/29769831/improvement-of-functional-ankle-properties-following-supplementation-with-specific-collagen-peptides-in-athletes-with-chronic-ankle-instability
#3
Patrick Dressler, Dominic Gehring, Denise Zdzieblik, Steffen Oesser, Albert Gollhofer, Daniel König
Following an initial ankle sprain it is not unlikely that chronic ankle instability (CAI) will develop. CAI is associated with impaired perceived functional and mechanical properties of the ligaments. Nutritional supplementation with collagen peptides has been shown to improve the functional and mechanical properties of the connective tissue. The purpose of this study was to investigate the effectiveness of specific collagen peptide supplementation (SCP) to improve ankle stability in athletes with CAI. 50 male and female athletes with CAI completed a randomized, double-blinded and placebo-controlled study with a daily oral administration of either 5 g SCP or 5 g placebo (Maltodextrin) over a period of six months...
June 2018: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/29769427/supplementation-with-camelina-oil-prevents-negative-changes-in-the-artery-in-orchidectomized-rats
#4
M Pawlowska-Olszewska, I Puzio, A P Harrison, L Borkowski, G Tymicki, D Grabos
The aim of the research was to examine the influence of orchidectomy on the elasticity and wall structure of the abdominal aorta in male rats and to check whether camelina oil treatment has an effect on aorta wall characteristics in orchidectomized rats. Forty 2-month-old male Wistar rats were used in the experiment: 10 animals underwent a sham testis repositioning operation (SHO) and 30 rats were orchidectomized (ORX). After the convalescence period, the SHO and ORX1 rats were given physiological saline intragastrically for 8 weeks; simultaneously, the other rats received camelina oil at the dose of 5 g/kg/b...
February 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29761823/erythropoietin-promotes-network-formation-of-transplanted-adipose-tissue-derived-microvascular-fragments
#5
P Karschnia, C Scheuer, A Heß, T Später, M D Menger, M W Laschke
The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls...
May 9, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29758546/chondrogenic-properties-of-collagen-type-xi-a-component-of-cartilage-extracellular-matrix
#6
Ang Li, Yiyong Wei, Clark Hung, Gordana Vunjak-Novakovic
Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix...
May 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29750046/improvement-of-dermal-parameters-in-aged-skin-after-oral-use-of-a-nutrient-supplement
#7
Flávia Alvim Sant'Anna Addor, Juliana Cotta Vieira, Camila Sirieiro Abreu Melo
Purpose: Skin aging is a progressive and degenerative process caused by a decrease in the physiological functions of the skin tissue. In addition, environmental factors as well as concomitant diseases and lifestyle (nutrition, sedentary lifestyle, smoking, etc) negatively impact the aging process. An association between oral administration of collagen peptides combined with vitamin C and extracts of Hibiscus sabdariffa and Aristotelia chilensis (Delphynol® ) (Eximia Firmalize Age complex® ) on dermal thickness was studied and the improvement in aging signs was evaluated...
2018: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/29746144/effects-of-allogenic-dermal-fibroblasts-on-rotator-cuff-healing-in-a-rabbit-model-of-chronic-tear
#8
Jieun Kwon, Yun Hee Kim, Sung-Min Rhee, Tae In Kim, Jimin Lee, Saewha Jeon, Joo Han Oh
BACKGROUND: The failure of rotator cuffs to heal after repair is an unresolved surgical issue. There have been substantial efforts, including the use of biological supplements, to enhance tendon healing. Dermal fibroblasts are a good candidate for tendon tissue engineering because they are similar to the tenocytes used for collagen synthesis. In addition, they are easily accessible because autologous dermal fibroblasts can be obtained from individual skin without major skin defects and allogenic dermal fibroblasts (ADFs) have already been commercialized in the field of skin engineering...
May 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29733549/toxicological-profiling-of-highly-purified-single-walled-carbon-nanotubes-with-different-lengths-in-the-rodent-lung-and-escherichia-coli
#9
Xiang Wang, Jae-Hyeok Lee, Ruibin Li, Yu-Pei Liao, Joohoon Kang, Chong Hyun Chang, Linda M Guiney, Vahid Mirshafiee, Linjiang Li, Jianqin Lu, Tian Xia, Mark C Hersam, André E Nel
Carbon nanotubes (CNTs) exhibit a number of physicochemical properties that contribute to adverse biological outcomes. However, it is difficult to define the independent contribution of individual properties without purified materials. A library of highly purified single-walled carbon nanotubes (SWCNTs) of different lengths is prepared from the same base material by density gradient ultracentrifugation, designated as short (318 nm), medium (789 nm), and long (1215 nm) SWCNTs. In vitro screening shows length-dependent interleukin-1β (IL-1β) production, in order of long > medium > short...
May 7, 2018: Small
https://www.readbyqxmd.com/read/29733414/effects-of-increasing-supplemental-dietary-zn-concentration-on-growth-performance-and-carcass-characteristics-in-finishing-steers-fed-ractopamine-hydrochloride
#10
Olivia N Genther-Schroeder, Mark E Branine, Stephanie L Hansen
Angus-cross steers (n = 288; 427 ± 0.4 kg) were utilized in a finishing study to evaluate the influence of increasing dietary Zn concentration on growth performance and carcass characteristics of steers fed ractopamine hydrochloride (RAC). In a randomized complete block design, steers were blocked by weight (6 steers/pen) and fed a dry-rolled corn-based diet for 79 d containing no supplemental Zn (CON; n = 8), 60 mg Zn/kg from ZnSO4 and no supplemental Zn-amino acid complex (ZnAA; ZnAA0; n = 8) or ZnAA0 diet supplemented with 60 (ZnAA60; n = 8), 90 (ZnAA90; n = 7), 120 (ZnAA120; n = 8), or 150 (ZnAA150; n = 8) mg Zn/kg DM from ZnAA...
May 4, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29732934/no-association-between-the-vitamin-d-pathway-gene-polymorphisms-and-bone-biomarkers-response-to-calcium-and-low-dose-calcitriol-supplementation-in-postmenopausal-chinese-women-a-one-year-prospective-study
#11
Jiemei Gu, Chun Wang, Hao Zhang, Hue Yue, Weiwei Hu, Jinwei He, Wenzhen Fu, Zhenlin Zhang
INTRODUCTION: The aim of the study was to explore the association between the Vitamin D pathway gene variations and the bone biomarkers response to calcium and low dose calcitriol supplementation in postmenopausal Chinese women. METHODS: A total of 110 healthy postmenopausal Chinese women (61.51±6.93 years) were enrolled. The participants were supplemented with calcium (600 mg/d) and calcitriol (0.25 μg/d), for 1 year. Four biomarkers, serum levels of beta C-terminal cross-linked telopeptides of type I collagen (β-CTX), amino-terminal propeptide of type I collagen (P1NP), parathyroid hormone (PTH) and 25-hydroxyvitamin D [25(OH)D] were measured at baseline and 12-month follow-up...
May 7, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29726717/anti-oxidant-anti-inflammatory-and-anti-apoptotic-effects-of-zinc-supplementation-in-experimental-induced-diabetic-rat-testes
#12
Neven M Aziz, Maha Yehia Kamel, Manar Mohamed, Sabreen Ahmed
AIM: One of the major obstacles that male diabetics may confront is sub-infertility and infertility. Thus the present study aims at investigating the role of co-administration of metformin and zinc (Zn) on the testes of streptozotocin (STZ)-induced diabetic rat. MATERIALS AND METHODS: Male albino rats were randomly divided into four groups: Control group; Diabetic non treated group; Diabetic + Metformin group, in which diabetic rats were treated by metformin 250 mg/kg orally once daily for 4 weeks; Diabetic + Metformin+ Zn group, in which diabetic rats treated for 4 weeks by metformin in combination with Zn 10mg/kg, orally once daily...
May 4, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29726103/chondrogenic-differentiation-of-human-chondrocytes-cultured-in-the-absence-of-ascorbic-acid
#13
M Adelaide Asnaghi, Ralph Duhr, Helen Quasnichka, Anthony P Hollander, Wael Kafienah, Ivan Martin, David Wendt
Bioreactor systems will likely play a key role in establishing regulatory compliant and cost-effective production systems for manufacturing engineered tissue grafts for clinical applications. However, the automation of bioreactor systems could become considerably more complex and costly due to the requirements for additional storage and liquid handling technologies if unstable supplements are added to the culture medium. Ascorbic acid (AA) is a bioactive supplement that is commonly presumed to be essential for the generation of engineered cartilage tissues...
May 4, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29714398/impact-of-human-platelet-lysate-on-the-expansion-and-chondrogenic-capacity-of-cultured-human-chondrocytes-for-cartilage-cell-therapy
#14
J G Sykes, J H Kuiper, J B Richardson, S Roberts, K T Wright, N J Kuiper
High hopes have been pinned on regenerative medicine strategies in order to prevent the progression of cartilage damage to osteoarthritis, particularly by autologous chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro monolayer expansion, a necessary step to obtain sufficient cell numbers, may be a key limitation in ACI. In this study, it was determined whether a shorter monolayer expansion approach could improve chondrogenic differentiation. The effects of two supplement types, foetal bovine serum (FBS) and Stemulate™ (a commercial source of human platelet lysate), on the expansion and re-differentiation potential of human chondrocytes, isolated from five individuals, were compared...
May 1, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29701488/effectiveness-of-a-dietary-supplement-containing-hydrolyzed-collagen-chondroitin-sulfate-and-glucosamine-in-pain-reduction-and-functional-capacity-in-osteoarthritis-patients
#15
Jordi Puigdellivol, Carme Comellas Berenger, Miguel Ángel Pérez Fernández, Juan M Cowalinsky Millán, Christian Carreras Vidal, Inés Gil Gil, Julio Martínez Pagán, Borja Ruiz Nieto, Francisco Jiménez Gómez, Francesc X Comas Figuerola, María E Aguilar Hernández
This observational, open, multicenter clinical trial with a single treatment group aimed to evaluate the effectiveness of a dietary supplement whose main ingredients are hydrolyzed gelatin, chondroitin sulfate, glucosamine sulfate, and devil's claw and bamboo extracts for pain reduction and improvement of functional capacities in patients with osteoarthritis (OA) of the knee and/or hip (REDART study). In all, 130 patients with OA recruited from Spanish hospitals received the dietary supplement for 6 months...
April 27, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29691830/stem-cells-in-osteochondral-tissue-engineering
#16
Eleonora Pintus, Matteo Baldassarri, Luca Perazzo, Simone Natali, Diego Ghinelli, Roberto Buda
Mesenchymal stem cells (MSCs) are pluripotent stem cells with the ability to differentiate into a variety of other connective tissue cells, such as chondral, bony, muscular, and tendon tissue. Bone marrow-derived MSCs are pluripotent cells that can differentiate among others into osteoblasts, adipocytes and chondrocytes.Bone marrow-derived cells may represent the future in osteochondral repair. A one-step arthroscopic technique is developed for cartilage repair, using a device to concentrate bone marrow-derived cells and collagen powder or hyaluronic acid membrane as scaffolds for cell support and platelet gel...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29691361/salvianolic-acid-b-suppresses-inflammatory-mediator-levels-by-downregulating-nf-%C3%AE%C2%BAb-in-a-rat-model-of-rheumatoid-arthritis
#17
Zeng-Bing Xia, Yong-Jian Yuan, Qiang-Hua Zhang, Heng Li, Ji-Lin Dai, Ji-Kang Min
BACKGROUND Salvianolic acid B (SB) is a major active phyto-component of the plant Radix Salvia miltiorrhiza, which is traditionally used to treat joint pain and arthritis. The present study examined the anti-rheumatoid arthritis efficacy of SB on collagen-induced rheumatoid arthritis (CIA) in a rat model. MATERIAL AND METHODS Forty-eight rats were divided into 4 groups: Control rats treated with saline (Group I), rats subjected to CIA induction by intradermal injection of bovine collagen II type at the tail (Group II), and rats subjected to CIA and supplemented with either 20 or 40 mg/kg of SB for 28 days (group III or IV)...
April 25, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29686516/structural-alterations-in-rat-myocardium-induced-by-chronic-l-arginine-and-l-name-supplementation
#18
Amal Abdussalam Ali A Hmaid, Milica Markelic, Vesna Otasevic, Sava Masovic, Aleksandra Jankovic, Bato Korac, Aleksandra Korac
Structural changes affecting cardiomyocyte function may contribute to the pathophysiological remodeling underlying cardiac function impairment. Recent reports have shown that endogenous nitric oxide (NO) plays an important role in this process. In order to examine the role of NO in cardiomyocyte remodeling, male rats were acclimated to room temperature (22 ± 1 °C) or cold (4 ± 1 °C) and treated with 2.25% l-arginine·HCl or 0.01% l-NAME (Nω -nitro-l-arginine methyl ester)·HCl for 45 days. Untreated groups served as controls...
March 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29684776/cyclic-uniaxial-compression-of-human-stem-cells-seeded-on-a-bone-biomimetic-nanocomposite-decreases-anti-osteogenic-commitment-evoked-by-shear-stress
#19
Walter Baumgartner, Isabelle Schneider, Samuel C Hess, Wendelin J Stark, Sonja Märsmann, Marzia Brunelli, Maurizio Calcagni, Paolo Cinelli, Johanna Buschmann
OBJECTIVE: Chemical supplementation of culture media to induce differentiation of adult stem cells seeded on a scaffold may mask other differentiation triggers such as scaffold stiffness, chemical composition or mechanical stimulation. However, stem cells can be differentiated towards osteoblasts without any supplementation given an appropriate osteogenic scaffold and an adequate mechanical stimulation. MATERIALS AND METHODS: Electrospun meshes of poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/aCaP) in a weight ratio of 60:40 were seeded with human adipose-derived stem cells (ASCs) and cultured in DMEM...
April 5, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29684347/developmental-endothelial-locus-1-prevents-development-of-peritoneal-adhesions-in-mice
#20
Yan Fu, Jiaywei Tsauo, Yucheng Sun, Zhe Wang, Kun Yung Kim, Seung-Hwan Lee, Dong-Young Kim, Feifeng Jing, Dahae Lim, Ho-Young Song, Hoon Hyun, Eun Young Choi
Postoperative peritoneal adhesions, fibrous bands formed in the peritoneal cavity following surgery, represent a common, challenging and costly problem faced by surgeons and patients, for which effective therapeutic options are lacking. Since aberrant inflammation is one of the key mechanisms underlying peritoneal adhesion formation, here we set out to study the role of developmental endothelial locus-1 (Del-1), which has been recently identified as an endogenous inhibitor of inflammation, in the formation of postoperative peritoneal adhesions using a mouse model of peritoneal adhesions induced by ischemic buttons...
April 24, 2018: Biochemical and Biophysical Research Communications
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