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The Quyen Nguyen, Mélanie Chenon, Fernando Vilela, Christophe Velours, Magali Aumont-Nicaise, Jessica Andreani, Paloma F Varela, Paola Llinas, Julie Ménétrey
Kinesin1 plays a major role in neuronal transport by recruiting many different cargos through its kinesin light chain (KLC). Various structurally unrelated cargos interact with the conserved tetratricopeptide repeat (TPR) domain of KLC. The N-terminal capping helix of the TPR domain exhibits an atypical sequence and structural features that may contribute to the versatility of the TPR domain to bind different cargos. We determined crystal structures of the TPR domain of both KLC1 and KLC2 encompassing the N-terminal capping helix and show that this helix exhibits two distinct and defined orientations relative to the rest of the TPR domain...
2017: PloS One
Fabio Carletti, Giuditta Gambino, Valerio Rizzo, Giuseppe Ferraro, Pierangelo Sardo
Cannabinoids (CB), transient receptors potential vanilloid type 1 (TRPV1) and nitric oxide (NO) were found to be interlinked in regulating some neuronal functions such as membrane excitability and synaptic transmission. TRPV1 play a fundamental role since it represents a synaptic target for CB that triggers several downstream cellular pathways. In this regard, recent evidence report that TRPV1 could influence NO production by modulating neuronal NO synthase (nNOS) activity. In the present research, we pointed to manipulate nNOS function to assess its role on TRPV1 signalling in hyperexcitability conditions elicited in the dentate gyrus of hippocampal formation...
October 3, 2017: Epilepsy Research
Alessandro Bellino, Giusy Lofrano, Maurizio Carotenuto, Giovanni Libralato, Daniela Baldantoni
Antibiotics are emerging pollutants released into the environment through wastewater and manure or effluents from livestock plants. Compared to the wide literature on the effects of antibiotics on the development of drug-resistant bacteria and on the adverse effects on animals and human beings, the effects on plants are less investigated. Here we evaluated the effects of four antibiotics (cloramphenicol: CAP, spiramycin: SPR, spectinomycin: SPT, vancomycin: VAN) belonging to different chemical groups, on seed germination and root development of tomato (Solanum lycopersicum L...
October 13, 2017: Ecotoxicology and Environmental Safety
Lingkai Zhang, Yongfeng Li, Libao Xie, Xiao Wang, Xulei Gao, Yuan Sun, Hua-Ji Qiu
Bivalent vaccines based on live attenuated viruses expressing a heterologous protein are an attractive strategy to address co-infections with various pathogens in the field. Considering the excellent efficacy and safety of the lapinized live attenuated vaccine C-strain (HCLV strain) of classical swine fever virus (CSFV), we proposed that C-strain has the potential as a viral vector for developing bivalent vaccines. To this end, we generated three recombinant viruses based on C-strain, one expressing the capsid (Cap) gene of porcine circovirus type 2 (PCV2) with the nuclear localization signal (NLS) (rHCLV-2ACap), and the other two expressing the PCV2 Cap gene without the NLS yet containing the signal peptide of the prolactin gene (rHCLV-pspCap) or that of the ubiquitin-specific peptidase gene (rHCLV-uspCap)...
October 16, 2017: Viruses
Jorge Espina-Casado, Alfonso Fernández-González, Ángel José Del Reguero-Huerga, Humberto Rodríguez-Solla, Marta Elena Díaz-García, Rosana Badía Laíño
One of the main drawbacks in the application of metal oxide nanoparticles as lubricant additives is their poor stability in organic media, despite the good anti-wear, friction-reducing and high load capacity properties described for these materials. In this work, we present a novel procedure to chemically cap the surface of ZrO2 nanoparticles (ZrO2NPs) with long hydrocarbon chains in order to obtain stable dispersions of ZrO2NPs in non-aqueous media without disrupting their attributes as lubricant additives...
October 16, 2017: Nanotechnology
Belinda L Needham, Margaret T Hicken, Ishtar O Govia, Colter Mitchell, Cleopatra M Abdou
Telomeres are the protective caps at the ends of eukaryotic chromosomes. Short telomere length is associated with morbidity and mortality among adults and may mark the biological impact of social experiences. Using archived dried blood spots from the Michigan Neonatal Biobank, this study examined markers of maternal social disadvantage (educational attainment, receipt of public assistance, marital status, and race/ethnicity) from linked birth certificates as predictors of telomere length at birth in a sample of 192 singleton neonates born to non-Hispanic black, non-Hispanic white, and Latina mothers aged 20-35 years...
2017: Biodemography and Social Biology
Huiyuan Wang, Manjie Zhang, Lingyu Zhang, Shengnan Li, Lu Li, Xiliang Li, Min Yu, Zhongcheng Mou, Tingting Wang, Chungang Wang, Zhongmin Su
A simple, mild and reproducible synthetic route was developed to fabricate the multifunctional Au@carbon/calcium phosphate core-shell nanoparticles (Au@C/CaP NPs), which act as an efficient nanoplatform for pH/near-infrared (NIR) dual-responsive drug delivery, X-ray computed tomography (CT) imaging and synergistic chemo-photothermal cancer therapy in vitro.
October 16, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Bin Qi, Chenchen Wu, Ling Xu, Wenjing Wang, Jin Cao, Jun Liu, Shuai Zhang, Detlef Gabel, Haibo Zhang, Xiaohai Zhou
A fundamental boron cluster, [closo-B12H12](2-), mainly known as an inert agent for boron neutron capture therapy, can also serve as a bi-functional reductant and a capping agent for the formation of monodispersed size-controlled gold nanoparticles (AuNPs) under mild reaction conditions. The obtained AuNPs remained thermo-dynamically stable over 3 months, and exhibited high performance in phase transfer and sensing of heavy metal ions.
October 16, 2017: Chemical Communications: Chem Comm
Marinella Mazzanti, Rory Kelly, Laurent Maron, Rosario Scopelliti
Organometallic multiple-decker sandwich complexes containing f-elements remain rare in spite of their attractive magnetic and electronic properties. The reduction of the CeIII siloxide complex, [KCeL4] (1; L = OSi(OtBu)3) with excess potassium in a THF/toluene mixture affords a rare tetradecker arene-bridged complex [K(2.2.2-crypt)]2[{(KL3Ce)(µ-η6:η6-C7H8)}2Ce] (3). The structure of 3 features a [Ce(C7H8)2] sandwich capped by [KL3Ce] moieties with a linear arrangement of the Ce ions. Structural parameters, UV-Vis data and DFT studies indicate the presence of CeII ions involved in δ bonding between the Ce cations and toluene dianions...
October 16, 2017: Angewandte Chemie
Susanna Torres Belmonte, Narly Benachi Sandoval
OBJECTIVE: To evaluate the impact of a socio-educational intervention to improve the quality of life of patients with fibromyalgia. METHOD: Out of 132 eligible candidates, 128 patients participated with a diagnosis of fibromyalgia (ACR 1990/2010), over 18 years of age, who did not have cognitive problems or mental disorders in acute phase and lived in the catchment area of the participating CAPs. The patients underwent intervention for 5 weeks to strengthen self-management of pain and improve quality of life...
October 13, 2017: Enfermería Clínica
Jae-Kwang Jung, Gi-Jeong Gwon, Sanjiv Neupane, Wern-Joo Sohn, Ki-Rim Kim, Ji-Youn Kim, Seo-Young An, Tae-Yub Kwon, Chang-Hyeon An, Youngkyun Lee, Jae-Young Kim, Jung-Hong Ha
INTRODUCTION: The aim of this study was to evaluate in vitro and ex vivo roles of bortezomib, a proteasome inhibitor that binds to the active site of the 26S proteasome, in tertiary dentin formation. METHODS: We established pulpal access cavity preparation that was treated with or without bortezomib before direct pulp capping with a calcium hydroxide-based material. We also analyzed bone morphogenetic protein (Bmp)- and Wnt-related signaling molecules using quantitative real-time polymerase chain reaction...
October 9, 2017: Journal of Endodontics
Charlotte Jeanneau, Patrick Laurent, Charlotte Rombouts, Thomas Giraud, Imad About
INTRODUCTION: Numerous studies reported dentin bridge formation after pulp capping with tricalcium silicates. By contrast, pulp capping with resins leads to pulp toxicity and inflammation. Hybrid materials made up of tricalcium silicates and resins have also been developed to be used in direct pulp capping. This work was designed to study the consequences of adding resins to tricalcium silicates by investigating TheraCal (BISCO, Lançon De Provence, France) and Biodentine (Septodont, Saint Maur des Fosses, France) interactions with the dental pulp...
October 9, 2017: Journal of Endodontics
Linzhong Wu, Qixuan Zhong, Di Yang, Min Chen, Huicheng Hu, Qi Pan, Haiyu Liu, Muhan Cao, Yong Xu, Baoquan Sun, Qiao Zhang
Recently, all-inorganic cesium lead halide (CsPbX3, X = Cl, Br, I) nanocrystals (NCs) have drawn wide attention because of their excellent optoelectronic properties and potential applications. However, one of the most significant challenges of such NCs is their low stability against protonic solvent. In this work, we demonstrate that by incorporating a highly-branched capping ligand, trioctylphosphine oxide (TOPO), into the traditional oleic acid/oleylamine system, monodisperse CsPbX3 NCs with excellent optoelectronic properties can be achieved at elevated temperatures (up to 260 oC)...
October 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Katherinne I Requejo, Anton Liopo, Paul J Derry, Eugene R Zubarev
A novel modification for the seedless synthesis of gold nanorods (AuNRs) has been developed. Nanomolar concentrations of 10 kDa poly(vinylpyrrolidone) (PVP) can be introduced to a growth solution containing 25, 50 or 100 mM CTAB to significantly reduce the dimensions of AuNRs. We found that PVP accelerates the growth rate of AuNRs by more than two times for nanorods grown in 50 and 100 mM CTAB solution. Additionally, there is a time-dependent effect of adding PVP to the nanorod growth solution that can be utilized to tune their aspect ratio...
October 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Hyeonjun Kim, Xiaomeng Tong, Sungyoung Choi, Jeong K Lee
The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of O₂, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (> or =0.5 mM). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately 24 μg bacteriochlorophyll a/ml resin...
October 14, 2017: Journal of Microbiology and Biotechnology
Kyumi Jeong, Doil Choi, Jundae Lee
The genomic region cosegregating with the genic male-sterile ms 1 gene of Capsicum annuum L. was delimited to a region of 869.9 kb on chromosome 5 through fine mapping analysis. A strong candidate gene, CA05g06780, a homolog of the Arabidopsis MALE STERILITY 1 gene that controls pollen development, was identified in this region. Genic male sterility caused by the ms 1 gene has been used for the economically efficient production of massive hybrid seeds in paprika (Capsicum annuum L.), a colored bell-type sweet pepper...
October 14, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Amy M Baetsen-Young, Matthew Vasher, Leann L Matta, Phil Colgan, Evangelyn C Alocilja, Brad Day
The interaction between gold nanoparticles (AuNPs) and nucleic acids has facilitated a variety of diagnostic applications, with further diversification of synthesis match bio-applications while reducing biotoxicity. However, DNA interactions with unique surface capping agents have not been fully defined. Using dextrin-capped AuNPs (d-AuNPs), we have developed a novel unamplified genomic DNA (gDNA) nanosensor, exploiting dispersion and aggregation characteristics of d-AuNPs, in the presence of gDNA, for sequence-specific detection...
October 7, 2017: Biosensors & Bioelectronics
Meng Zhu, Chuen Yan Leung, Marta N Shahbazi, Magdalena Zernicka-Goetz
Establishment of cell polarity in the mammalian embryo is fundamental for the first cell fate decision that sets aside progenitor cells for both the new organism and the placenta. Yet the sequence of events and molecular mechanism that trigger this process remain unknown. Here, we show that de novo polarisation of the mouse embryo occurs in two distinct phases at the 8-cell stage. In the first phase, an apical actomyosin network is formed. This is a pre-requisite for the second phase, in which the Par complex localises to the apical domain, excluding actomyosin and forming a mature apical cap...
October 13, 2017: Nature Communications
Ashraf Ali, Faiz Ali, Won Jo Cheong
The sedimentation procedure has been adopted in production of ground silica monolith particles to improve chromatographic separation efficiency of the resultant phase. First, silica monolith particles have been successfully prepared in a large scale by a sol-gel process followed by grinding. The particles after calcination were separated by sedimentation into three zones using an Imhoff sedimentation cone. The particles of the bottom zone were derivatized with a C18 ligand and end-capped. The sedimentation process was found to not only eliminate troublesome minute particles but also narrow down the particle size distribution...
October 6, 2017: Journal of Chromatography. A
Olga Bibikova, Julian Haas, Ángela I López-Lorente, Alexey Popov, Matti Kinnunen, Yury Ryabchikov, Andrei Kabashin, Igor Meglinski, Boris Mizaikoff
Plasmonic anisotropic nanoparticles possess a number of hot spots on their surface due to the presence of sharp edges, tips or vertices, leading to a high electric field strength surrounding the nanostructures. In this paper, we explore different plasmonic nanostructures, including anisotropic gold nanostars (AuNSts) and spherical gold nanoparticles, in surface-enhanced infrared absorption spectroscopy (SEIRAS) in an attenuated total reflection (ATR) configuration. In our experiments, we observed up to 10-times enhancement of the infrared (IR) absorption of thioglycolic acid (TGA) and up to 2-times enhancement of signals for bovine serum albumin (BSA) protein on plasmonic nanostructure-based films deposited on a silicon (Si) internal reflection element (IRE) compared to bare Si IRE...
October 16, 2017: Analytica Chimica Acta
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