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https://www.readbyqxmd.com/read/28345580/differential-roles-of-caspase-1-and-caspase-11-in-infection-and-inflammation
#1
Si Ming Man, Rajendra Karki, Benoit Briard, Amanda Burton, Sebastien Gingras, Stephane Pelletier, Thirumala-Devi Kanneganti
Caspase-1, also known as interleukin-1β (IL-1β)-converting enzyme (ICE), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. On activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-IL-1β and pro-IL-18 into their biologically active forms. "ICE(-/-)" or "Casp1(-/-)" mice generated using 129 embryonic stem cells carry a 129-associated inactivating passenger mutation on the caspase-11 locus, essentially making them deficient in both caspase-1 and caspase-11...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28343937/co-infection-of-blood-borne-viruses-in-blood-donors-a-cross-sectional-study-from-north-india
#2
Ravi C Dara, Aseem K Tiwari, Dinesh Arora, Geet Aggarwal, Ganesh Singh Rawat, Jyoti Sharma, Devi Prasad Acharya, Gunjan Bhardwaj
BACKGROUND: There are several studies on prevalence of individual infectious disease markers (mono-infection) in donors but none on prevalence of coinfection. Co-infection is significant as it leads to accelerated disease progression. We, therefore, evaluated the prevalence of co-infection among blood donors. MATERIALS AND METHODS: The cross-sectional analysis was conducted in blood donors. All donors were tested for anti-HIV I and II, HBsAg, anti-HBC IgM, anti-HCV, Malaria and syphilis by chemiluminescence and ID-NAT assay...
March 6, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28343771/characterization-of-human-monoclonal-antibodies-that-neutralize-multiple-poliovirus-serotypes
#3
Rama Devudu Puligedda, Diana Kouiavskaia, Fetweh H Al-Saleem, Chandana Devi Kattala, Usman Nabi, Hamid Yaqoob, V Sandeep Bhagavathula, Rashmi Sharma, Konstantin Chumakov, Scott K Dessain
Following the eradication of wild poliovirus (PV), achieving and maintaining a polio-free status will require eliminating potentially pathogenic PV strains derived from the oral attenuated vaccine. For this purpose, a combination of non-cross-resistant drugs, such as small molecules and neutralizing monoclonal antibodies (mAbs), may be ideal. We previously isolated chimpanzee and human mAbs capable of neutralizing multiple PV types (cross-neutralization). Here, we describe three additional human mAbs that neutralize types 1 and 2 PV and one mAb that neutralizes all three types...
March 23, 2017: Vaccine
https://www.readbyqxmd.com/read/28338612/pesticide-knowledge-and-safety-practices-among-farm-workers-in-kuwait-results-of-a-survey
#4
Mustapha F A Jallow, Dawood G Awadh, Mohammed S Albaho, Vimala Y Devi, Binson M Thomas
The unsafe and indiscriminate use of pesticides in agriculture represents a major hazard to the environment and human health. The aim of this study was to assess the levels of knowledge, attitude and practices of Kuwaiti farmers regarding the safe use of pesticides. A total of 250 farmers participated in this study through in-depth interviews and observations on-farm. The majority of the farmers acknowledged that pesticides were harmful to their health (71%) and the environment (65%). However, farmers' level of knowledge of pesticide safety is insufficient...
March 24, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28333896/relationship-of-optic-nerve-structure-and-function-to-peripapillary-vessel-density-measurements-of-optical-coherence-tomography-angiography-in-glaucoma
#5
Harsha L Rao, Zia S Pradhan, Robert N Weinreb, Srilakshmi Dasari, Mohammed Riyazuddin, Sharanya Raveendran, Narendra K Puttaiah, Jayasree P Venugopal, Dhanaraj A S Rao, Sathi Devi, Kaweh Mansouri, Carroll A B Webers
PURPOSE: To evaluate the sectoral and global structure-structure (vessel density-retinal nerve fiber layer thickness) and structure-function (vessel density-visual sensitivity loss) relationships of peripapillary vessel density measurements on optical coherence tomography angiography in primary open-angle glaucoma and to determine if fractional polynomial (FP) models characterize the relationships better than linear models. MATERIALS AND METHODS: In a cross-sectional study, structure-structure and structure-function relationships of peripapillary vessel densities were determined in 227 eyes of 143 subjects (63 control and 164 primary open-angle glaucoma eyes) who had undergone standard automated perimetry and optical coherence tomography testing within 6 months of each other, using linear and FP models...
March 22, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28330805/photodynamic-therapeutic-efficacy-of-symmetrical-diiodinated-squaraine-in-in-vivo-skin-cancer-models
#6
M S Soumya, D Devi Gayathri, K M Shafeekh, Suresh Das, Annie Abraham
BACKGROUND: Photodynamic therapy (PDT) has clinical approval for use as a minimally invasive therapeutic procedure that is able to exert selective cytotoxic activity toward malignant cells. The dye selected for our study, symmetrical diiodinated squaraine, is one of the newly developed photosensitizers. The study is designed to determine the efficacy of PDT mediated by symmetrical diiodinated squaraine in skin tumor induced Swiss albino mice. METHODS: Skin tumor was induced in mice with dimethyl benzanthracene (DMBA) and croton oil...
March 19, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28330237/biocatalytic-activity-of-aspergillus-niger-xylanase-in-paper-pulp-biobleaching
#7
A Sridevi, A Sandhya, G Ramanjaneyulu, G Narasimha, P Suvarnalatha Devi
Xylanase is a hemicellulase enzyme that catalyses the hydrolysis of xylan to xylose which is widely used in processing of feed, pulp and paper. It is produced by many microorganisms especially filamentous fungi like Trichoderma and Aspergillus. A potential xylanolytic fungal isolate Aspergillus niger was isolated from forest soils of Tirumala, AP, India, and its crude enzyme was checked for its potential in paper bleaching. Under submerged fermentation, production of xylanase, cellulase, biomass, total protein and sugar released were analysed after 7 days of incubation at room temperature...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330183/purification-and-characterization-of-lipoxygenase-from-mung-bean-vigna-radiata-l-germinating-seedlings
#8
Raveendra Aanangi, Kasi Viswanath Kotapati, Bhagath Kumar Palaka, Thyagaraju Kedam, Nirmala Devi Kanika, Dinakara Rao Ampasala
This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified LOX has molecular mass of approximately 97 kDa and showed high activity with linoleic acid than linolenic acid and arachidonic acid...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327173/a-new-paradigm-for-aedes-spp-surveillance-using-gravid-ovipositing-sticky-trap-and-ns1-antigen-test-kit
#9
Sai Ming Lau, Tock H Chua, Wan-Yussof Sulaiman, Sylvia Joanne, Yvonne Ai-Lian Lim, Shamala Devi Sekaran, Karuthan Chinna, Balan Venugopalan, Indra Vythilingam
BACKGROUND: Dengue remains a serious public health problem in Southeast Asia and has increased 37-fold in Malaysia compared to decades ago. New strategies are urgently needed for early detection and control of dengue epidemics. METHODS: We conducted a two year study in a high human density dengue-endemic urban area in Selangor, where Gravid Ovipositing Sticky (GOS) traps were set up to capture adult Aedes spp. mosquitoes. All Aedes mosquitoes were tested using the NS1 dengue antigen test kit...
March 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28326485/preliminary-assessment-on-potentials-of-probiotic-b-subtilis-rx7-and-b-methylotrophicus-c14-strains-as-an-immune-modulator-in-salmonella-challenged-weaned-pigs
#10
Santi Devi Upadhaya, Suresh Kumar Shanmugam, Dae Kyung Kang, In Ho Kim
A total of 40 crossbred weaned piglets (28 days old; [Landrace × Yorkshire] × Duroc) were used for preliminary assessment on potentials of Bacillus-based probiotics as an immune modulator in a Salmonella Typhimurium challenge model in a 3-week experiment. Pigs were randomly allotted to four experimental diets according to their initial body weight (9.21 ± 1.1 kg) and sex (10 pigs per treatment; 5 barrows and 5 gilts). The dietary treatments were basal diet (CON), basal diet + oral administration of Salmonella enterica ser...
March 21, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28326203/esthetic-rehabilitation-of-anterior-teeth-with-copy-milled-restorations-a-report-of-two-cases
#11
Sapna Rani, Jyoti Devi, Chandan Jain, Parul Mutneja, Mahesh Verma
Digitalization has become part and parcel of contemporary prosthodontics with the probability of most of the procedures being based on the digital techniques in the near future. This digital revolution started in the latter half of the 20th century by converting analog objects/signals into digital bits and bytes. Recent developments in all-ceramic materials and systems of computer-aided designing and computer-aided manufacturing (CAD/CAM), copy milling, and so forth offer excellent esthetics and superb biocompatibility...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/28323393/evaluation-of-antidiabetic-activity-of-biologically-synthesized-silver-nanoparticles-agnps-using-pouteria-sapota-on-stz-induced-diabetic-rats
#12
Sathya Prabhu, Shanmugam Vinodhini, Chakravarthy Elenchezhiyan, Devi Rajeswari
BACKGROUND: Medicinal plants and green synthesis of silver nanoparticles (AgNPs) have proven to be potent source effective agents in the treatment of diabetes mellitus. The present study is focused on the green synthesis of AgNPs from aqueous leaf extract of Pouteria sapota (P. sapota) in order to evaluate the in-vitro and in-vivo anti-diabetic property of aqueous extracts and their synthesized AgNPs. METHODS: AgNPs were biologically synthesized under ambient conditions from an aqueous leaf extract of P...
March 21, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/28320234/gelidiella-acerosa-protects-against-a%C3%AE-25-35-induced-toxicity-and-memory-impairment-in-swiss-albino-mice-an-in-vivo-report
#13
Syad Arif Nisha, Kasi Pandima Devi
CONTEXT: Alzheimer's disease (AD) is believed to develop due to deposition of β-amyloid (Aβ) peptide. Hence, efforts are being made to develop potent drug that target amyloid hypothesis. OBJECTIVE: The present study explores the effect of the seaweed Gelidiella acerosa (Forsskål) Feldmann & Hamel (Gelidiellaceae) against Aβ 25-35 peptide in Swiss albino mice. MATERIALS AND METHODS: The animals were administered through intracerebroventricular (ICV) injection with the Aβ 25-35 peptide (10 μg/10 μL/ICV site) on 21st day of the pretreatment of G...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28316948/denture-care-practices-and-perceived-denture-status-among-complete-denture-wearers
#14
Krishnam Raju V Kosuru, Ganji Devi, Vikram Grandhi, Kumar K Prasan, Manoj K Yasangi, Mannem Dhanalakshmi
AIMS AND OBJECTIVES: Considerable importance to oral health care was lacking in India, and oral health neglect continues to exist, which is evident in the low utilization rates and poor oral health status. Conventional dentures are the most common alternative in restoration of lost teeth. Without proper denture care practices, there is an increased risk of developing a multitude of problems. The aim of this study is to assess the denture care practices among complete denture wearers in East Godavari district, Andhra Pradesh...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28316442/alloimmunization-in-autoimmune-hemolytic-anemia-patient-the-differential-adsorption-approach
#15
Ravi C Dara, Aseem Kumar Tiwari, Dinesh Arora, Subhasis Mitra, Devi Prasad Acharya, Geet Aggarwal, Jyoti Sharma
Patients of β-thalassemia major are dependent on regular blood transfusions for their entire lifetime. Development of antibodies against red blood cell (RBC) antigen which may be alloantibody or autoantibody, several times as a result of frequent red cell component transfusions, further complicates the subsequent transfusion therapy. Among the autoantibodies, warm-reactive autoantibodies are commoner and interfere in the pretransfusion testing. These RBC autoantibodies present in patient's serum potentially react with all the cells of antibody identification panel giving "pan-reactive" picture and making alloantibody identification complex...
January 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28316415/molecular-and-pathological-studies-on-natural-cases-of-bovine-theileriosis
#16
Anamika Gupta, Kuldip Gupta, Geeta Devi Leishangthem, Mandeep Singh Bal, N K Sood, Amarjit Singh
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28303831/strengths-and-limitations-of-various-screening-methods-for-carbapenem-resistant-enterobacteriaceae-including-new-method-recommended-by-clinical-and-laboratory-standards-institute-2017-a-tertiary-care-experience
#17
Agila Kumari Pragasam, Balaji Veeraraghavan, Yamuna Devi Bakthavatchalam, Radha Gopi, Raziya Fathima Aslam
Carbapenemase-mediated carbapenem resistance is a major concern across the world. Rapid detection of carbapenemase-producing organisms is of great importance in clinical settings. However, it is essential to have a test with good sensitivity and specificity. The aim of the study was to compare the performance of RAPIDEC® CARBA NP and modified carbapenem inactivation method (mCIM) recommended by Clinical and Laboratory Standards Institute guideline 2017. A total of ninety carbapenem resistant Escherichia coli and Klebsiella pneumoniae have been tested...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303819/prevalence-and-genetic-mechanisms-of-antimicrobial-resistance-in-staphylococcus-species-a-multicentre-report-of-the-indian-council-of-medical-research-antimicrobial-resistance-surveillance-network
#18
Sunanda Rajkumar, Sujatha Sistla, Meerabai Manoharan, Madhan Sugumar, Niveditha Nagasundaram, Subhash Chandra Parija, Pallab Ray, Yamuna Devi Bakthavatchalam, Balaji Veeraraghavan, Arti Kapil, Kamini Walia, V C Ohri
PURPOSE: Routine surveillance of antimicrobial resistance (AMR) is an essential component of measures aimed to tackle the growing threat of resistant microbes in public health. This study presents a 1-year multicentre report on AMR in Staphylococcus species as part of Indian Council of Medical Research-AMR surveillance network. MATERIALS AND METHODS: Staphylococcus species was routinely collected in the nodal and regional centres of the network and antimicrobial susceptibility testing was performed against a panel of antimicrobials...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303784/a-rapid-method-for-the-quantitative-determination-of-34-pesticides-in-nonalcoholic-carbonated-beverages-using-liquid-liquid-extraction-coupled-to-dispersive-solid-phase-cleanup-followed-by-gas-chromatography-with-tandem-mass-spectrometry
#19
Satyajeet Rai, Madhuri Devi Gullapalli, Anshuman Srivastava, Hussain Shaik, Mohammed Haris Siddiqui, Mohana Krishna Reddy Mudiam
An economical, rapid, and sensitive multiresidue method using liquid-liquid extraction (LLE) coupled with dispersive SPE (dSPE) cleanup was developed for the quantitative determination of 34 multiclass multiresidue (MCMR) pesticides (14 organochlorines, eight organophosphates, 10 synthetic pyrethroids, and two herbicides) in nonalcoholic carbonated beverages (cola, orange, lemon-lime, and citra) using GC with tandem MS. The procedure mainly involved LLE by dichloromethane and dSPE cleanup in the presence of magnesium sulfate, primary secondary amine, and C<sub>18</sub>...
March 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28303149/qtl-hotspots-for-early-vigor-and-related-traits-under-dry-direct-seeded-system-in-rice-oryza-sativa-l
#20
Uma M Singh, Shailesh Yadav, Shilpi Dixit, P Janaki Ramayya, M Nagamallika Devi, K Anitha Raman, Arvind Kumar
Strong seedling vigor is desirable trait in dry direct-seeded rice (DSR) for enhancing crop establishment and the ability to compete against weeds. A set of 253 BC3F4 lines derived from cross between Swarna and Moroberekan was phenotyped for early vigor (EV) and 8 related traits viz., early uniform emergence (EUE), shoot length (SHL), stem length (SL), shoot fresh weight (SFW), total fresh weight (TFW), shoot dry weight (SDW), total dry weight (TDW), and root dry weight (RDW). Composite interval mapping analysis using genotypic data from 194 SNP markers identified six genomic regions associated with traits on chromosomes 3, 4, 5, and 6 with phenotypic variance ranging from 2...
2017: Frontiers in Plant Science
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