keyword
MENU ▼
Read by QxMD icon Read
search

Mass spectrometry images

keyword
https://www.readbyqxmd.com/read/28538661/mass-spectrometry-based-profiling-and-imaging-of-various-ginsenosides-from-panax-ginseng-roots-at-different-ages
#1
Jae Won Lee, Seung-Heon Ji, Young-Seob Lee, Doo Jin Choi, Bo-Ram Choi, Geum-Soog Kim, Nam-In Baek, Dae Young Lee
(1) Background: Panax ginseng root is one of the most important herbal products, and the profiling of ginsenosides is critical for the quality control of ginseng roots at different ages in the herbal markets. Furthermore, interest in assessing the contents as well as the localization of biological compounds has been growing. The objective of this study is to carry out the mass spectrometry (MS)-based profiling and imaging of ginsenosides to assess ginseng roots at different ages; (2) Methods: Optimal ultra performance liquid chromatography coupled to quadrupole time of flight/MS (UPLC-QTOF/MS) was used to profile various ginsenosides from P...
May 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28527668/imaging-mass-spectrometry-ims-of-cortical-lipids-from-preclinical-to-severe-stages-of-alzheimer-s-disease
#2
E Gónzalez de San Román, I Manuel, M T Giralt, I Ferrer, R Rodríguez-Puertas
Alzheimer's disease (AD) is a progressive neurodegenerative disease affecting millions of patients worldwide. Previous studies have demonstrated alterations in the lipid composition of lipid extracts from plasma and brain samples of AD patients. However, there is no consensus regarding the qualitative and quantitative changes of lipids in brains from AD patients. In addition, the recent developments in imaging mass spectrometry methods are leading to a new stage in the in situ analysis of lipid species in brain tissue slices from human postmortem samples...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28523824/laser-desorption-ionisation-mass-spectrometry-imaging-of-european-yew-taxus-baccata-on-gold-nanoparticle-enhanced-target
#3
Adrian Arendowski, Tomasz Ruman
INTRODUCTION: European yew (Taxus baccata) is a plant known to man for centuries as it produces many interesting and important metabolites. These chemical compounds were repeatedly analysed by various analytical techniques, but none of the methods used so far allowed the localisation of the chemical compounds within the tissue and also correlation between plant morphology and its biochemistry. OBJECTIVE: Visualisation of the spatial distribution of yew metabolites with nanoparticle-based mass spectrometry imaging...
May 19, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28523509/the-future-in-disease-models-for-mass-spectrometry-imaging-ethical-issues-and-the-way-forward
#4
Rebecca E Day, Ieva Palubeckaite
Mass Spectrometry Imaging (MSI) has evolved into a valuable tool for research into and the diagnosis of disease pathology. The ability to perform multiplex analysis of a wide range of molecules (e.g., proteins, lipids, and metabolites) simultaneously per tissue section while retaining the histological structure of the sample allows molecular information and tissue morphology to be correlated, thus increasing our understanding of a particular disease. Further development of MSI is required to improve suitability to the alternative models available, so that the combined approach can successfully provide the information required in disease characterization and prevention...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523508/rodent-whole-body-sectioning-and-maldi-mass-spectrometry-imaging
#5
Paul J Trim
Here, we describe a method for obtaining whole-body MALDI imaging data. MALDI imaging provides chemical compound-specific information not attainable with conventional histology techniques. The specificity of mass spectrometry with the addition of spatial information makes this a very powerful technique, especially for the analysis of endogenous and exogenous small molecules. This chapter will provide the reader with a comprehensive description of the techniques involved in obtaining high-quality MALDI mass spectrometry imaging (MSI) data from large tissue sections...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523507/tof-sims-parallel-imaging-ms-ms-of-lipid-species-in-thin-tissue-sections
#6
Anne Lisa Bruinen, Gregory L Fisher, Ron M A Heeren
Unambiguous identification of detected species is essential in complex biomedical samples. To date, there are not many mass spectrometry imaging techniques that can provide both high spatial resolution and identification capabilities. A new and patented imaging tandem mass spectrometer, exploiting the unique characteristics of the nanoTOF II (Physical Electronics, USA) TOF-SIMS TRIFT instrument, was developed to address this.Tandem mass spectrometry is based on the selection of precursor ions from the full secondary ion spectrum (MS(1)), followed by energetic activation and fragmentation, and collection of the fragment ions to obtain a tandem MS spectrum (MS(2))...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523506/maldi-mass-spectrometry-profiling-and-imaging-applied-to-the-analysis-of-latent-fingermarks
#7
Robert Bradshaw
Latent fingermarks are derived from a transfer of material from the fingertips to a surface upon contact. Traditionally, fingermarks are employed for biometric identification of individuals based on matching of the pattern of the ridges. However, in recent years, there has been a stark increase in the use of advanced analytical techniques in order to obtain additional information, specifically the chemical composition of the residue. Understanding the complexity of the endogenous and exogenous content of fingermarks could be extremely useful in allowing further development of enhancement techniques currently used in forensic scenarios by identifying potential target molecules...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523505/mass-spectrometry-imaging-of-drugs-of-abuse-in-hair
#8
Bryn Flinders, Eva Cuypers, Tiffany Porta, Emmanuel Varesio, Gérard Hopfgartner, Ron M A Heeren
Hair testing is a powerful tool routinely used for the detection of drugs of abuse. The analysis of hair is highly advantageous as it can provide prolonged drug detectability versus that in biological fluids and chronological information about drug intake based on the average growth of hair. However, current methodology requires large amounts of hair samples and involves complex time-consuming sample preparation followed by gas or liquid chromatography coupled with mass spectrometry. Mass spectrometry imaging is increasingly being used for the analysis of single hair samples, as it provides more accurate and visual chronological information in single hair samples...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523504/laser-ablation-inductively-coupled-plasma-mass-spectrometry-imaging-of-plant-metabolites
#9
Callie Seaman
LA-ICP-MS (Laser Ablation Inductively Coupled Plasma Mass Spectrometry) is a highly sensitive isotopic and elemental analytical technique that has a wide application for solid samples such as plant material. Within this chapter we describe how to prepare leaf material, seeds, and fruit for 2D elemental distribution maps, preparation of reference materials, method condition optimization, special resolution calculation, and the limit of detection.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523501/peptide-imaging-maximizing-peptide-yield-optimization-of-the-peptide-mass-fingerprint
#10
Ekta Patel
In Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI), identification of observed proteins remains a challenge primarily due to the drop in sensitivity of time-of-flight (TOF) mass spectrometers beyond the mass range of 25-35 kDa (Francese et al. High Throughput Screen 12: 156-174, 2009) and inadequate mass resolving power at those molecular weights. To counteract this, a bottom-up proteomic approach is often employed. Proteolysis digests proteins into smaller peptide fragments, typically between 500 and 3000 Da which are easier to detect with a higher mass accuracy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523500/desi-mass-spectrometry-imaging-msi
#11
Emmanuelle Claude, Emrys A Jones, Steven D Pringle
Desorption Electrospray Ionization (DESI) mass spectrometry is a technique that allows chemical information to be obtained directly from a wide range of surfaces. Using a 2D stage, DESI can be implemented in an imaging mode whereby MS spectra are collected by rastering the spray across the whole surface. Here, we describe the implementation and optimization of DESI imaging for metabolites and lipids from tissue sections using oa-TOF mass spectrometers.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523499/droplet-based-liquid-extraction-for-spatially-resolved-microproteomics-analysis-of-tissue-sections
#12
Maxence Wisztorski, Jusal Quanico, Julien Franck, Benoit Fatou, Michel Salzet, Isabelle Fournier
Obtaining information on protein content while keeping their localization on tissue or organ is of importance in different domains to understand pathophysiological processes. There is increasing interest in studying the microenvironment and heterogeneity of tumors, which currently is difficult with existing proteomics techniques. The advent of new techniques, like MALDI Mass Spectrometry Imaging, made a significant progress in the last decade but is characterized by a number of inherent drawbacks. One of these is the limited identification of proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523498/maldi-msi-analysis-of-cytological-smears-the-study-of-thyroid-cancer
#13
Niccolò Mosele, Andrew Smith, Manuel Galli, Fabio Pagni, Fulvio Magni
Fine needle aspiration (FNA) biopsies are the current gold-standard for the preoperative evaluation of thyroid nodules. However, a significant number of them (15-30%) are unable to be affirmatively diagnosed and are given an "indeterminate for malignancy" final report, meaning that the malignant nature of the thyroid nodule remains unknown and the recommended therapeutic approach is total thyroidectomy. Furthermore, cytomorphological evaluation of biopsies taken post-surgery indicates that approximately 80% of nodules within this group of patients are in fact benign, and the total thyroidectomy unwarranted...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523497/maldi-msi-of-lipids-in-human-skin
#14
Philippa J Hart, Malcolm R Clench
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) is now a well-established technique for imaging analysis of sectioned biological tissues. One of the growing areas of interest is in the analysis of skin. MALDI-MSI can provide a wealth of information from within sections of skin. This includes information on the distribution of pharmaceuticals following topical treatments, through to the examination of the composition of different skin layers and studies of proteomic, lipidomic, and metabolomic responses to disease, wounds, and external stimuli...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523496/imaging-ms-of-rodent-ocular-tissues-and-the-optic-nerve
#15
David M G Anderson, Wendi Lambert, David J Calkins, Zsolt Ablonczy, Rosalie K Crouch, Richard M Caprioli, Kevin L Schey
The visual system is comprised of many specialized cell types that are essential for relaying sensory information about an animal's surroundings to the brain. The cells present in ocular tissue are notoriously delicate, making it particularly challenging to section thin slices of unfixed tissue. Maintaining the morphology of the native tissue is crucial for accurate observations by either conventional staining techniques or in this instance matrix-assisted laser desorption ionization (MALDI IMS) or imaging using mass spectrometry...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28523495/fresh-frozen-versus-formalin-fixed-paraffin-embedded-for-mass-spectrometry-imaging
#16
Ekta Patel
Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry Imaging (MSI) is fast becoming an industry leading technique as a means of investigating the distributions of protein and peptide molecules directly from sections of tissue. Developing protocols for the analysis of FFPE tissue opens up numerous opportunities for novel biomarker discovery, due to the large number of tissue banks containing FFPE biopsy samples. This chapter reports the analysis of both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues using such protocols...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28522250/ppargamma-agonists-rescue-increased-phosphorylation-of-fgf14-at-s226-in-the-tg2576-mouse-model-of-alzheimer-s-disease
#17
Wei-Chun J Hsu, Norelle C Wildburger, Sigmund J Haidacher, Miroslav N Nenov, Oluwarotimi Folorunso, Aditya K Singh, Brent C Chesson, Whitney F Franklin, Ibdanelo Cortez, Rovshan G Sadygov, Kelly T Dineley, Jay S Rudra, Giulio Taglialatela, Cheryl F Lichti, Larry Denner, Fernanda Laezza
BACKGROUND: Cognitive impairment in humans with Alzheimer's disease (AD) and in animal models of Aβ-pathology can be ameliorated by treatments with the nuclear receptor peroxisome proliferator-activated receptor-gamma (PPARγ) agonists, such as rosiglitazone (RSG). Previously, we demonstrated that in the Tg2576 animal model of AD, RSG treatment rescued cognitive deficits and reduced aberrant activity of granule neurons in the dentate gyrus (DG), an area critical for memory formation...
May 15, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28521163/application-for-oxytetracycline-wastewater-pretreatment-by-fenton-iron-mud-based-cathodic-anodic-electrolysis-ceramic-granular-fillers
#18
Feilong Zhang, Qinyan Yue, Yuan Gao, Baoyu Gao, Xing Xu, Zhongfei Ren, Yang Jin
In this study, Fenton iron mud applied as main raw material of cathodic-anodic-electrolysis ceramic granular fillers (ICMF) in a continuous reactor, which were used to pretreat oxytetracycline (OTC) wastewater. The ICMF was characterized by Scanning Electron Microscope and Energy Dispersive Spectrometer analysis. The effects of pH value, hydraulic retention time, OTC concentrations and aeration on removal efficiency of total organic carbon (TOC) and OTC were studied. The degradation byproducts of OTC were analyzed by UV-2450, High Performance Liquid Chromatography and Liquid Chromatography-mass Spectrometry...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28520405/morphology-driven-control-of-metabolite-selectivity-using-nanostructure-initiator-mass-spectrometry
#19
Jian Gao, Katherine B Louie, Philipp Steinke, Benjamin P Bowen, Markus de Raad, Ronald N Zuckermann, Gary Siuzdak, Trent R Northen
Nanostructure-initiator mass spectrometry (NIMS) is a laser desorption/ionization analysis technique based on the vaporization of a nanostructure trapped liquid 'initiator' phase. Here we report an intriguing relationship between NIMS surface morphology and analyte selectivity. Scanning electron microscopy and spectroscopic ellipsometry were used to characterize the surface morphologies of a series of NIMS substrates generated by anodic electrochemical etching method. Mass spectrometry imaging was applied to compare NIMS sensitivity of these various surfaces towards the analysis of diverse analytes...
May 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28517936/in-situ-hydrogel-conditioning-of-tissue-samples-to-enhance-the-drug-s-sensitivity-in-ambient-mass-spectrometry-imaging
#20
Xiaowei Song, Zhigang Luo, Xin Li, Tiegang Li, Zhonghua Wang, Chenglong Sun, Luojiao Huang, Ping Xie, Xiaoyu Liu, Jiuming He, Zeper Abliz
Ion suppression from the tissue matrix has a severe effect on the mass spectrometry imaging (MSI) of drugs. This problem hinders further applications of MSI in preclinical drug research and development. In this study, an in situ hydrogel conditioning method was developed to enhance the sensitivity of air-flow-assisted desorption electrospray ionization (AFADESI)-MSI. Instead of the traditional wash-up or digestion treatment in solvent, this method used a solid phase hydrogel to "wash" tissue sections. It was demonstrated that this in situ hydrogel conditioning method improve the drug signal by as much as 2- to 25-folds in MSI, especially for hydrophobic compounds...
May 18, 2017: Analytical Chemistry
keyword
keyword
33714
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"