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https://www.readbyqxmd.com/read/29143845/pulse-laser-induced-fragmentation-of-carbon-quantum-dots-a-structural-analysis
#1
Han-Wei Chu, Ju-Yi Mao, Chia-Wen Lien, Pang-Hung Hsu, Yu-Jia Li, Jui-Yang Lai, Tai-Chia Chiu, Chih-Ching Huang
Carbon quantum dots (CQDs) have attracted enormous interest in recent years owing to their low cytotoxicity, excellent biocompatibility and strong fluorescence. They have been successfully employed in sensor, bio-imaging, and drug carrier applications. A complete understanding of their core-surface structure is essential for tuning their physical and chemical properties for various applications. Conventional characterizations of CQDs are conducted with electron microscopy or spectroscopy, such as transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and Raman spectroscopy...
November 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/29139291/an-integrative-approach-to-decipher-the-chemical-antagonism-between-the-competing-endophytes-paraconiothyrium-variabile-and-bacillus-subtilis
#2
Marine Vallet, Quentin P Vanbellingen, Tingting Fu, Jean-Pierre Le Caer, Serge Della-Negra, David Touboul, Katherine R Duncan, Bastien Nay, Alain Brunelle, Soizic Prado
An integrative approach combining traditional natural products chemistry, molecular networking, and mass spectrometry imaging has been undertaken to decipher the molecular dialogue between the fungus Paraconiothyrium variabile and the bacterium Bacillus subtilis, which were isolated as endophytes from the conifer Cephalotaxus harringtonia and are characterized by a strong and mutual antibiosis. From this study, we highlight that bacterial surfactins and a fungal tetronic acid are involved in such competition and that the fungus is able to hydrolyze surfactins to fight against the bacterial partner...
November 15, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29137123/proteomic-characterization-of-the-venom-of-five-bombus-thoracobombus-species
#3
Nezahat Pınar Barkan, Mustafa Bilal Bayazit, Duygu Ozel Demiralp
Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees (Bombus sp.) is largely unknown. The Thoracobombus subgenus of Bombus sp. is a diverse subgenus represented by 14 members across Turkey. In this study, we sought out to proteomically characterize the venom of five Thoracobombus species by using bottom-up proteomic techniques...
November 11, 2017: Toxins
https://www.readbyqxmd.com/read/29136820/fluorescent-ligand-fishing-combination-with-in-situ-imaging-and-characterizing-to-screen-hsp-90-inhibitors-from-curcuma-longa-l-based-on-inp-zns-quantum-dots-embedded-mesoporous-nanoparticles
#4
Yue Hu, Anchen Fu, Zhaoyi Miao, Xiaojing Zhang, Tianlin Wang, An Kang, Jinjun Shan, Dong Zhu, Wei Li
Although ligand fishing has been shown to be an efficient technique for the identification of bioactive components from complex mixtures such as natural products, it cannot be applied to biomedical image processing. Herein, a specific fluorescent ligand fishing combined with in situ imaging approach is presented for the identification of heat shock protein 90 (Hsp 90) inhibitors from complex matrixes, Curcuma longa L., using N-terminus immobilized Hsp 90α functionalized InP/ZnS quantum dots embedded mesoporous nanoparticles (i...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136815/quantitative-study-of-zinc-and-metallothioneins-in-the-human-retina-and-rpe-cells-by-mass-spectrometry-based-methodologies
#5
Sara Rodríguez-Menéndez, Beatriz Fernández, Montserrat García, Lydia Álvarez, Maria Luisa Fernández, Alfredo Sanz-Medel, Miguel Coca-Prados, Rosario Pereiro, Héctor González-Iglesias
The retina contains the highest concentration of zinc in the human eye and it is primarily associated with the retinal pigment epithelium (RPE). Metallothioneins (MTs) are the main cytosolic zinc-ion-binding proteins, exerting a tight control in the number of atoms of Zn-bound to the MTs related with their antioxidant and neuroprotective functions. In order to study the Zn-MT system in retina and RPE, we have implemented mass spectrometry (MS)-based technologies: two complementary element detection methodologies (HPLC- and laser ablation (LA)-ICP-MS) have been successfully employed to study metal content in the human eye as well as to perform speciation studies of Zn-MTs...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136808/an-approach-for-quantification-of-platinum-distribution-in-tissues-by-la-icp-ms-imaging-using-isotope-dilution-analysis
#6
I Moraleja, M L Mena, A Lázaro, B Neumann, A Tejedor, N Jakubowski, M M Gómez-Gómez, D Esteban-Fernández
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been revealed as a convenient technique for trace elemental imaging in tissue sections, providing elemental 2D distribution at a quantitative level. For quantification purposes, in the last years several approaches have been proposed in the literature such as the use of CRMs or matrix matched standards. The use of Isotope Dilution (ID) for quantification by LA-ICP-MS has been also described, being mainly useful for bulk analysis but not feasible for spatial measurements so far...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29134222/water-soluble-au-nanoclusters-for-multiplexed-mass-spectrometry-imaging
#7
Jinan Li, Jing Liu, Zheyi Liu, Yuan Tan, Xiaoyan Liu, Fangjun Wang
Homogeneous and water-soluble Au nanoclusters with high UV photon adsorption and high biocompatibility were utilized to enhance the detection sensitivity and lateral resolution of multiplexed mass spectrometry imaging based on laser desorption/ionization.
November 14, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29131162/the-3d-orbisims-label-free-metabolic-imaging-with-subcellular-lateral-resolution-and-high-mass-resolving-power
#8
Melissa K Passarelli, Alexander Pirkl, Rudolf Moellers, Dmitry Grinfeld, Felix Kollmer, Rasmus Havelund, Carla F Newman, Peter S Marshall, Henrik Arlinghaus, Morgan R Alexander, Andy West, Stevan Horning, Ewald Niehuis, Alexander Makarov, Colin T Dollery, Ian S Gilmore
We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; under 200 nm for inorganic species and under 2 μm for biomolecules) with the high mass-resolving power of an Orbitrap (>240,000 at m/z 200). This allows exogenous and endogenous metabolites to be visualized in 3D with subcellular resolution. We imaged the distribution of neurotransmitters-gamma-aminobutyric acid, dopamine and serotonin-with high spectroscopic confidence in the mouse hippocampus...
November 13, 2017: Nature Methods
https://www.readbyqxmd.com/read/29130504/mass-spectrometry-based-metabolomics-targeting-the-crosstalk-between-gut-microbiota-and-brain-in-neurodegenerative-disorders
#9
REVIEW
Hemi Luan, Xian Wang, Zongwei Cai
Metabolomics seeks to take a "snapshot" in a time of the levels, activities, regulation and interactions of all small molecule metabolites in response to a biological system with genetic or environmental changes. The emerging development in mass spectrometry technologies has shown promise in the discovery and quantitation of neuroactive small molecule metabolites associated with gut microbiota and brain. Significant progress has been made recently in the characterization of intermediate role of small molecule metabolites linked to neural development and neurodegenerative disorder, showing its potential in understanding the crosstalk between gut microbiota and the host brain...
November 12, 2017: Mass Spectrometry Reviews
https://www.readbyqxmd.com/read/29127342/chemical-analysis-of-morphological-changes-in-lysophosphatidic-acid-treated-ovarian-cancer-cells
#10
Karen A Bailey, Yuliya Klymenko, Peter E Feist, Amanda B Hummon, M Sharon Stack, Zachary D Schultz
Ovarian cancer (OvCa) cells are reported to undergo biochemical changes at the cell surface in response to treatment with lysophosphatidic acid (LPA). Here we use scanning electron microscopy (SEM) and multiplex coherent anti-Stokes Raman scattering (CARS) imaging via supercontinuum excitation to probe morphological changes that result from LPA treatment. SEM images show distinct shedding of microvilli-like features upon treatment with LPA. Analysis of multiplex CARS images can distinguish between molecular components, such as lipids and proteins...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29126286/deep-learning-for-tumor-classification-in-imaging-mass-spectrometry
#11
Jens Behrmann, Christian Etmann, Tobias Boskamp, Rita Casadonte, Jörg Kriegsmann, Peter Maass
Motivation: Tumor classification using Imaging Mass Spectrometry (IMS) data has a high potential for future applications in pathology. Due to the complexity and size of the data, automated feature extraction and classification steps are required to fully process the data. Since mass spectra exhibit certain structural similarities to image data, deep learning may offer a promising strategy for classification of IMS data as it has been successfully applied to image classification. Results: Methodologically, we propose an adapted architecture based on deep convolutional networks to handle the characteristics of mass spectrometry data, as well as a strategy to interpret the learned model in the spectral domain based on a sensitivity analysis...
November 8, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29124306/a-new-strategy-for-metal-labeling-of-glycan-structures-in-antibodies
#12
Lena Ruhe, Stefanie Ickert, Sebastian Beck, Michael W Linscheid
Quantitative analysis of complex proteins is a challenging task in modern bioanalytical chemistry. Commonly available isotope labels are still suffering from limitations and drawbacks, whereas new metal labels open numerous possibilities in mass spectrometric analyses. In this work, we have developed a new metal labeling strategy to tag glycan structures of proteins, more particularly antibodies. The oligosaccharide glycans were selectively trimmed to the last N-acetylglucosamine to which an artificial azide containing galactose residue was bound...
November 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29124084/second-harmonic-generation-imaging-of-collagen-in-ancient-bone
#13
B Thomas, D McIntosh, T Fildes, L Smith, F Hargrave, M Islam, T Thompson, R Layfield, D Scott, B Shaw, C L Burrell, S Gonzalez, S Taylor
Second-harmonic generation imaging (SHG) captures triple helical collagen molecules near tissue surfaces. Biomedical research routinely utilizes various imaging software packages to quantify SHG signals for collagen content and distribution estimates in modern tissue samples including bone. For the first time using SHG, samples of modern, medieval, and ice age bones were imaged to test the applicability of SHG to ancient bone from a variety of ages, settings, and taxa. Four independent techniques including Raman spectroscopy, FTIR spectroscopy, radiocarbon dating protocols, and mass spectrometry-based protein sequencing, confirm the presence of protein, consistent with the hypothesis that SHG imaging detects ancient bone collagen...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/29122912/top-down-microproteomics-bridged-to-maldi-ms-imaging-reveals-the-molecular-physiome-of-brain-regions
#14
Vivian Delcourt, Julien Franck, Jusal Quanico, Jean-Pascal Gimeno, Maxence Wisztorski, Antonella Raffo Romero, Firas Kobeissy, Xavier Roucou, Michel Salzet, Isabelle Fournier
Tissue top-down microproteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the microproteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region...
November 9, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29119981/magnetic-bead-based-peptide-extraction-methodology-for-tissue-imaging
#15
William T Andrews, Susan B Skube, Amanda B Hummon
MALDI-TOF imaging mass spectrometry (IMS) is a common technique used for analyzing tissue samples, as it allows the user to detect multiple different analytes simultaneously. However, the detection and analysis of these analytes may sometimes be hampered due to the presence of contaminants in the tissue microenvironment, which leads to ion suppression. This challenge necessitates the development of an active extraction technique to selectively isolate analytes of interest without compromising their spatial localization within a tissue sample...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29117637/secondary-ion-mass-spectrometry-imaging-and-multivariate-data-analysis-reveal-co-aggregation-patterns-of-populus-trichocarpa-leaf-surface-compounds-on-a-micrometer-scale
#16
Purva Kulkarni, Mina Dost, Özgül Demir Bulut, Alexander Welle, Sebastian Böcker, Wilhelm Boland, Aleš Svatoš
Spatially resolved analysis of a multitude of compound classes has become feasible with the rapid advancement in mass spectrometry imaging strategies. In this study, we present a protocol that combines high lateral resolution TOF-SIMS imaging with a multivariate data analysis (MVA) approach to probe the complex leaf surface chemistry of Populus trichocarpa. Here, epicuticular waxes (EWs) found on the adaxial leaf surface of Populus trichocarpa were blotted on silicon wafers and imaged using time-of-flight secondary ion mass spectrometry (TOF-SIMS) at 10 μm and 1 μm lateral resolution...
November 8, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29117483/a-manganese-based-alternative-to-gadolinium-contrast-enhanced-mr-angiography-excretion-pharmacokinetics-and-metabolism
#17
Eric M Gale, Hsiao-Ying Wey, Ian Ramsay, Yi-Fen Yen, David E Sosnovik, Peter Caravan
Purpose To compare intravascular contrast enhancement produced by the manganese-based magnetic resonance (MR) imaging contrast agent manganese-N-picolyl-N,N',N'-trans-1,2-cyclohexenediaminetriacetate (Mn-PyC3A) to gadopentetate dimeglumine (Gd-DTPA) and to evaluate the excretion, pharmacokinetics, and metabolism of Mn-PyC3A. Materials and Methods Contrast material-enhanced MR angiography was performed in baboons (Papio anubis; n = 4) by using Mn-PyC3A and Gd-DTPA. Dynamic imaging was performed for 60 minutes following Mn-PyC3A injection to monitor distribution and elimination...
November 8, 2017: Radiology
https://www.readbyqxmd.com/read/29115333/an-apolipoprotein-enriched-biomolecular-corona-switches-the-cellular-uptake-mechanism-and-trafficking-pathway-of-lipid-nanoparticles
#18
L Digiacomo, F Cardarelli, D Pozzi, S Palchetti, M A Digman, E Gratton, A L Capriotti, M Mahmoudi, G Caracciolo
Following exposure to biological milieus (e.g. after systemic administration), nanoparticles (NPs) get covered by an outer biomolecular corona (BC) that defines many of their biological outcomes, such as the elicited immune response, biodistribution, and targeting abilities. In spite of this, the role of BC in regulating the cellular uptake and the subcellular trafficking properties of NPs has remained elusive. Here, we tackle this issue by employing multicomponent (MC) lipid NPs, human plasma (HP) and HeLa cells as models for nanoformulations, biological fluids, and target cells, respectively...
November 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/29114726/concordance-between-different-amyloid-immunoassays-and-visual-amyloid-positron-emission-tomographic-assessment
#19
Shorena Janelidze, Josef Pannee, Alvydas Mikulskis, Ping Chiao, Henrik Zetterberg, Kaj Blennow, Oskar Hansson
Importance: Visual assessment of amyloid positron emission tomographic (PET) images has been approved by regulatory authorities for clinical use. Several immunoassays have been developed to measure β-amyloid (Aβ) 42 in cerebrospinal fluid (CSF). The agreement between CSF Aβ42 measures from different immunoassays and visual PET readings may influence the use of CSF biomarkers and/or amyloid PET assessment in clinical practice and trials. Objective: To determine the concordance between CSF Aβ42 levels measured using 5 different immunoassays and visual amyloid PET analysis...
November 6, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/29113129/atmospheric-pressure-plasma-polymerization-synthesis-and-characterization-of-polyaniline-films-doped-with-and-without-iodine
#20
Choon-Sang Park, Eun Young Jung, Dong Ha Kim, Do Yeob Kim, Hyung-Kun Lee, Bhum Jae Shin, Dong Ho Lee, Heung-Sik Tae
Although polymerized aniline (polyaniline, PANI) with and without iodine (I₂) doping has already been extensively studied, little work has been done on the synthesis of PANI films using atmospheric pressure plasma (APP) deposition. Therefore, this study characterized pure and I₂-doped PANI films synthesized using an advanced APP polymerization system. The I₂ doping was conducted ex-situ and using an I₂ chamber method following the APP deposition. The pure and I₂-doped PANI films were structurally analyzed using field emission scanning electron microscope (FE-SEM), atomic force microscope (AFM), X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and time of flight secondary ion mass spectrometry (ToF-SIMS) studies...
November 6, 2017: Materials
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