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https://www.readbyqxmd.com/read/28432654/laser-desorption-ionization-mass-spectrometric-imaging-of-endogenous-lipids-from-rat-brain-tissue-implanted-with-silver-nanoparticles
#1
Ludovic Muller, Kathrine Baldwin, Damon C Barbacci, Shelley N Jackson, Aurélie Roux, Carey D Balaban, Bruce E Brinson, Michael I McCully, Ernest K Lewis, J Albert Schultz, Amina S Woods
Mass spectrometry imaging (MSI) of tissue implanted with silver nanoparticulate (AgNP) matrix generates reproducible imaging of lipids in rodent models of disease and injury. Gas-phase production and acceleration of size-selected 8 nm AgNP is followed by controlled ion beam rastering and soft landing implantation of 500 eV AgNP into tissue. Focused 337 nm laser desorption produces high quality images for most lipid classes in rat brain tissue (in positive mode: galactoceramides, diacylglycerols, ceramides, phosphatidylcholines, cholesteryl ester, and cholesterol, and in negative ion mode: phosphatidylethanolamides, sulfatides, phosphatidylinositol, and sphingomyelins)...
April 21, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28431006/regulation-of-oxidized-platelet-lipidome-implications-for-coronary-artery-disease
#2
Madhumita Chatterjee, Dominik Rath, Jörg Schlotterbeck, Johannes Rheinlaender, Britta Walker-Allgaier, Nada Alnaggar, Monika Zdanyte, Iris Müller, Oliver Borst, Tobias Geisler, Tilman E Schäffer, Michael Lämmerhofer, Meinrad Gawaz
Aims: Hyperlipidaemia enhances susceptibility to thrombosis, while platelet oxidixed LDL (oxLDL) binding in acute coronary syndrome (ACS) correlates with activation status. This study explores the platelet lipidome in symptomatic coronary artery disease (CAD) patients and the functional consequences of the chemokine CXCL12 and its receptors CXCR-4/-7 on lipid uptake in platelets. Methods and results: Platelet-oxLDL detected by flow cytometry was enhanced (P = 0...
April 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28430048/a-spinal-epidural-abscess-due-to-streptobacillus-moniliformis-infection-following-a-rat-bite-case-report
#3
Alexander Hammer, Dorit Wolff, Walter Geißdörfer, Michael Schrey, Renate Ziegler, Hans-Herbert Steiner, Christian Bogdan
The authors describe the case of a 40-year-old man suffering from an epidural abscess in the thoracic spine due to a rarely isolated pathogen, Streptobacillus moniliformis, the causative agent of rat bite fever. Besides diffuse abdominal pain, ataxia, paresthesia, hypesthesia, and enhanced reflexes of the lower extremities, the patient suffered from a decreased sensation of bladder filling. His history was also positive for a rat bite 6 weeks earlier. Magnetic resonance imaging showed an epidural, space-occupying lesion compressing the spinal cord at the vertebral levels of T6-8...
April 21, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/28428226/rapid-throughput-analysis-of-gabaa-receptor-subtype-modulators-and-blockers-using-disbac1-3-membrane-potential-red-dye
#4
Atefeh Mousavi Nik, Brandon Pressly, Vikrant Singh, Shane Antrobus, Susan Hulsizer, Michael A Rogawski, Heike Wulff, Isaac N Pessah
Fluorometric Imaging Plate Reader (FLIPR®) membrane potential dye (FMP-Red-Dye) is a proprietary tool for basic discovery and high throughput drug screening for G-protein coupled receptors and ion channels. We optimized and validated this potentiometric probe to assay functional modulators of heterologous expressed GABAA receptor (GABAAR) isoforms (synaptic α1β3γ2, extrasynaptic α4β3δ, and β3 homopentomers). High-resolution mass spectrometry identified FMP-Red-dye as DisSBAC1(3). GABAAR expressing cells equilibrated with FMP-Red-Dye exhibited depolarized equilibrium membrane (Em) potentials compared to GABAAR-null cells...
April 20, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28426978/visualizing-spatial-distribution-of-small-molecules-in-the-rhubarb-stalk-rheum-rhabarbarum-by-surface-transfer-mass-spectrometry-imaging
#5
Joanna Nizioł, Justyna Sekuła, Tomasz Ruman
Laser desorption/ionization mass spectrometry imaging (LDI-MSI) with gold nanoparticle-enhanced target (AuNPET) was used for visualization of small molecules in the rhubarb stalk (Rheum rhabarbarum L.). Analysis was focused on spatial distribution of biologically active compounds which are found in rhubarb species. Detected compounds belong to a very wide range of chemical compound classes such as anthraquinone derivatives and their glucosides, stilbenes, anthocyanins, flavonoids, polyphenols, organic acids, chromenes, chromanones, chromone glycosides and vitamins...
April 17, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28425543/in-situ-nuclear-magnetic-resonance-microimaging-of-live-biofilms-in-a-microchannel
#6
R S Renslow, M J Marshall, A E Tucker, W B Chrisler, X-Y Yu
Biofilms are comprised of microbial cells and an extracellular polymeric substance (EPS) matrix that supports interactions between community members and with the local environment. The highly hydrated EPS matrix makes the application of many biofilm visualization techniques difficult. Hence, to better visualize how biofilms interact with their environment, there is a need for imaging techniques to monitor hydrated state biofilm dynamics. We employed an in situ dynamic approach to construct label-free images of biofilms...
April 20, 2017: Analyst
https://www.readbyqxmd.com/read/28424781/postembedding-decalcification-of-mineralized-tissue-sections-preserves-the-integrity-of-implanted-biomaterials-and-minimizes-number-of-experimental-animals
#7
Thaqif El Khassawna, Diaa Eldin S Daghma, Sabine Stoetzel, Seemun Ray, Stefanie Kern, Deeksha Malhan, Volker Alt, Ulrich Thormann, Anja Henß, Marcus Rohnke, Annette Stengel, Fathi Hassan, Stefan Maenz, Klaus D Jandt, Michael Diefenbeck, Matthias Schumacher, Michael Gelinsky, Katrin Susanne Lips, Christian Heiss
Bone histology of decalcified or undecalcified samples depends on the investigation. However, in research each method provides different information to answer the scientific question. Decalcification is the first step after sample fixation and governs what analysis is later feasible on the sections. Besides, decalcification is favored for immunostaining and in situ hybridization. Otherwise, sample decalcification can be damaging to bone biomaterials implants that contains calcium or strontium. On the other hand, after decalcification mineralization cannot be assessed using histology or imaging mass spectrometry...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28422753/steroid-metabolome-analysis-reveals-prevalent-glucocorticoid-excess-in-primary-aldosteronism
#8
Wiebke Arlt, Katharina Lang, Alice J Sitch, Anna S Dietz, Yara Rhayem, Irina Bancos, Annette Feuchtinger, Vasileios Chortis, Lorna C Gilligan, Philippe Ludwig, Anna Riester, Evelyn Asbach, Beverly A Hughes, Donna M O'Neil, Martin Bidlingmaier, Jeremy W Tomlinson, Zaki K Hassan-Smith, D Aled Rees, Christian Adolf, Stefanie Hahner, Marcus Quinkler, Tanja Dekkers, Jaap Deinum, Michael Biehl, Brian G Keevil, Cedric H L Shackleton, Jonathan J Deeks, Axel K Walch, Felix Beuschlein, Martin Reincke
BACKGROUND: Adrenal aldosterone excess is the most common cause of secondary hypertension and is associated with increased cardiovascular morbidity. However, adverse metabolic risk in primary aldosteronism extends beyond hypertension, with increased rates of insulin resistance, type 2 diabetes, and osteoporosis, which cannot be easily explained by aldosterone excess. METHODS: We performed mass spectrometry-based analysis of a 24-hour urine steroid metabolome in 174 newly diagnosed patients with primary aldosteronism (103 unilateral adenomas, 71 bilateral adrenal hyperplasias) in comparison to 162 healthy controls, 56 patients with endocrine inactive adrenal adenoma, 104 patients with mild subclinical, and 47 with clinically overt adrenal cortisol excess...
April 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28421168/contribution-of-the-a-baumannii-a1s_0114-gene-to-the-interaction-with-eukaryotic-cells-and-virulence
#9
Soraya Rumbo-Feal, Astrid Pérez, Theresa A Ramelot, Laura Álvarez-Fraga, Juan A Vallejo, Alejandro Beceiro, Emily J Ohneck, Brock A Arivett, María Merino, Steven E Fiester, Michael A Kennedy, Luis A Actis, Germán Bou, Margarita Poza
Genetic and functional studies showed that some components of the Acinetobacter baumannii ATCC 17978 A1S_0112-A1S_0119 gene cluster are critical for biofilm biogenesis and surface motility. Recently, our group has shown that the A1S_0114 gene was involved in biofilm formation, a process related with pathogenesis. Confirming our previous results, microscopy images revealed that the ATCC 17978 Δ0114 derivative lacking this gene was unable to form a mature biofilm structure. Therefore, other bacterial phenotypes were analyzed to determine the role of this gene in the pathogenicity of A...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28416487/metabolic-markers-and-statistical-prediction-of-serous-ovarian-cancer-aggressiveness-by-ambient-ionization-mass-spectrometry-imaging
#10
Marta Sans, Kshipra M Gharpure, Robert Tibshirani, Jialing Zhang, Li Liang, Jinsong Liu, Jonathan H Young, Robert Dood, Anil K Sood, Livia Eberlin
Ovarian high-grade serous carcinoma (HGSC) results in the highest mortality among gynecological cancers, developing rapidly and aggressively. Dissimilarly, serous borderline ovarian tumors (BOT) can progress into low-grade serous carcinomas and have relatively indolent clinical behavior. The underlying biological differences between HGSC and BOT call for accurate diagnostic methodologies and tailored treatment options, and identification of molecular markers of aggressiveness could provide valuable biochemical insights and improve disease management...
April 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28415473/preparation-of-a-novel-bioavailable-curcuminoid-formulation-cureit%C3%A2-using-polar-nonpolar-sandwich-pns-technology-and-its-characterization-and-applications
#11
Augustine Amalraj, Shintu Jude, Karthik Varma, Joby Jacob, Sreeraj Gopi, Oluwatobi S Oluwafemi, Sabu Thomas
Health benefits of curcuminoid are highly limited due to their poor aqueous solubility, very low systemic bioavailability, fast metabolic alterations and rapid elimination. In this study, a novel bioavailable curcuminoid formulation Cureit™ was prepared by using Polar-Nonpolar-Sandwich (PNS) technology with complete natural turmeric matrix (CNTM). The synthesized bioavailable curcuminoid formulation Cureit™ was characterizations by Nuclear magnetic resonance spectroscopy (NMR), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infra-red (IR), current-voltage (I-V) study, Quadrupole Time-of-Flight Mass Spectrometry (Q-TOF), differential scanning calorimeter (DSC) and thermogravimetric analysis (TGA)...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28411641/nanoarmoring-of-proteins-by-conjugation-to-block-copolymer-micelles
#12
Nisaraporn Suthiwangcharoen, Ramanathan Nagarajan
The creation of polymer nanoparticles with protein functionality is of great interest to many applications such as targeted drug or gene delivery, diagnostic imaging, cancer theranostics, delivery of protein therapeutics, sensing chemical and biomolecular analytes in complex environments, and design of protective clothing resembling a second skin. Many approaches to achieving this goal are being explored in the current literature. In this chapter, we describe a relatively simple and flexible approach of conjugating the protein to an amphiphilic block copolymer and creating polymer nanoparticles with protein functionality by taking advantage of the intrinsic self-assembly behavior of the amphiphilic block copolymer...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28411106/mass-spectrometry-imaging-shows-major-derangements-in-neurogranin-and-in-purine-metabolism-in-the-triple-knockout-3%C3%A3-tg-alzheimer-mouse-model
#13
Clara Esteve, Emrys A Jones, Douglas B Kell, Hervé Boutin, Liam A McDonnell
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) can simultaneously measure hundreds of biomolecules directly from tissue. Using different sample preparation strategies, proteins and metabolites have been profiled to study the molecular changes in a 3×Tg mouse model of Alzheimer's disease. In comparison with wild-type (WT) control mice MALDI-MSI revealed Alzheimer's disease-specific protein profiles, highlighting dramatic reductions of a protein with m/z 7560, which was assigned to neurogranin and validated by immunohistochemistry...
April 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28411018/charge-tagging-liquid-chromatography-mass-spectrometry-methodology-targeting-oxysterol-diastereoisomers
#14
William J Griffiths, Thomas Hearn, Peter J Crick, Jonas Abdel-Khalik, Alison Dickson, Eylan Yutuc, Yuqin Wang
The introduction of a hydroxy group to the cholesterol skeleton introduces not only the possibility for positional isomers but also diastereoisomers, where two or more isomers have different configurations at one or more of the stereocentres but are not mirror images. The differentiation of diastereoisomers is important as differing isomers can have differing biochemical properties and are formed via different biochemical pathways. Separation of diasterioisomers is not always easy by chromatographic methods Here we demonstrate, by application of charge-tagging and derivatisation with the Girard P reagent, the separation and detection of biologically relevant diastereoisomers using liquid chromatography - mass spectrometry with multistage fragmentation...
April 11, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28409937/spatial-and-temporal-localization-of-flavonoid-metabolites-in-strawberry-fruit-fragaria-%C3%A3-ananassa
#15
Anna Christina Crecelius, Dirk Hoelscher, Thomas Hoffmann, Bernd Schneider, Thilo C Fischer, Magda-Viola Hanke, Henry Flachowsky, Wilfried Schwab, Ulrich S Schubert
Flavonoids are important metabolites in strawberries (Fragaria × ananassa), since they accomplish an extensive collection of physiological functions and are valuable for human health. However, their localization within the fruit tissue has not been extensively explored. Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to shed light on the spatial distribution of flavonoids during fruit development. One wild-type (WT) and two transgenic lines were compared, where in the transgenics enzymes anthocyanidin reductase (ANRi) and flavonol synthase (FLSi), respectively were down-regulated using an RNAi based silencing approach...
April 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28406322/mass-spectrometry-based-proteomic-exploration-of-the-human-immune-system-focus-on-the-inflammasome-global-protein-secretion-and-t-cells
#16
Tuula A Nyman, Martina B Lorey, Wojciech Cypryk, Sampsa Matikainen
The immune system is our defense system against microbial infections and tissue injury, and understanding how it works in detail is essential for developing drugs for different diseases. Mass spectrometry-based proteomics can provide in-depth information on the molecular mechanisms involved in immune responses. Areas covered: Summarized are the key immunology findings obtained with MS-based proteomics in the past five years, with a focus on inflammasome activation, global protein secretion, mucosal immunology, immunopeptidome and T cells...
April 13, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28405806/rearrangement-of-potassium-ions-and-kv1-1-kv1-2-potassium-channels-in-regenerating-axons-following-end-to-end-neurorrhaphy-ionic-images-from-tof-sims
#17
Chiung-Hui Liu, Hung-Ming Chang, Tsung-Huan Wu, Li-You Chen, Yin-Shuo Yang, To-Jung Tseng, Wen-Chieh Liao
The voltage-gated potassium channels Kv1.1 and Kv1.2 that cluster at juxtaparanodal (JXP) regions are essential in the regulation of nerve excitability and play a critical role in axonal conduction. When demyelination occurs, Kv1.1/Kv1.2 activity increases, suppressing the membrane potential nearly to the equilibrium potential of K(+), which results in an axonal conduction blockade. The recovery of K(+)-dependent communication signals and proper clustering of Kv1.1/Kv1.2 channels at JXP regions may directly reflect nerve regeneration following peripheral nerve injury...
April 12, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28405197/mass-spectrometry-based-molecular-3d-cartography-of-plant-metabolites
#18
Dimitrios J Floros, Daniel Petras, Clifford A Kapono, Alexey V Melnik, Tie-Jun Ling, Rob Knight, Pieter C Dorrestein
Plants play an essential part in global carbon fixing through photosynthesis and are the primary food and energy source for humans. Understanding them thoroughly is therefore of highest interest for humanity. Advances in DNA and RNA sequencing and in protein and metabolite analysis allow the systematic description of plant composition at the molecular level. With imaging mass spectrometry, we can now add a spatial level, typically in the micrometer-to-centimeter range, to their compositions, essential for a detailed molecular understanding...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28405187/identification-of-cerebral-metal-ion-imbalance-in-the-brain-of-aging-octodon-degus
#19
Nady Braidy, Anne Poljak, Chris Marjo, Helen Rutlidge, Anne Rich, Bat-Erdene Jugder, Tharusha Jayasena, Nibaldo C Inestrosa, Perminder S Sachdev
The accumulation of redox-active transition metals in the brain and metal dyshomeostasis are thought to be associated with the etiology and pathogenesis of several neurodegenerative diseases, and Alzheimer's disease (AD) in particular. As well, distinct biometal imaging and role of metal uptake transporters are central to understanding AD pathogenesis and aging but remain elusive, due inappropriate detection methods. We therefore hypothesized that Octodon degus develop neuropathological abnormalities in the distribution of redox active biometals, and this effect may be due to alterations in the expression of lysosomal protein, major Fe/Cu transporters, and selected Zn transporters (ZnTs and ZIPs)...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28401432/feasibility-assessment-of-a-maldi-fticr-imaging-approach-for-the-3d-reconstruction-of-a-mouse-lung
#20
E Ellen Jones, Cristine Quiason, Stephanie Dale, Sheerin K Shahidi-Latham
Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has proven to be a quick, robust, and label-free tool to produce two-dimensional (2D) ion-density maps representing the distribution of a variety of analytes across a tissue section of interest. In addition, three-dimensional (3D) imaging mass spectrometry workflows have been developed that are capable of visualizing these same analytes throughout an entire volume of a tissue rather than a single cross-section. Until recently, the use of Fourier transform ion cyclotron resonance (FTICR) mass spectrometers for 3D volume reconstruction has been impractical due to software limitations, such as inadequate capacity to manipulate the extremely large data files produced during an imaging experiment...
April 11, 2017: Journal of the American Society for Mass Spectrometry
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