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https://www.readbyqxmd.com/read/28733204/the-polymorphism-xrcc1-arg194trp-and-8-hydroxydeoxyguanosine-increased-susceptibility-to-arsenic-related-renal-cell-carcinoma
#1
Yu-Mei Hsueh, Ying-Chin Lin, Wei-Jen Chen, Chao-Yuan Huang, Horng-Sheng Shiue, Yeong-Shiau Pu, Chi-Hung Chen, Chien-Tien Su
This study was designed to explore the relationship between X-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms and renal cell carcinoma (RCC) and to investigate whether individuals with an XRCC1 risk genotype, a high level of 8-OHdG or a high urinary total arsenic concentration have a modified odds ratio (OR) of RCC. We recruited 180 RCC patients and 360 age- and sex-matched controls from a hospital-based pool. Image-guided biopsy or surgical resection of renal tumors was performed on RCC patients for pathological verification...
July 18, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28731323/analyzing-liposomal-drug-delivery-systems-in-three-dimensional-cell-culture-models-using-maldi-imaging-mass-spectrometry
#2
Jessica K Lukowski, Eric M Weaver, Amanda B Hummon
Cancer chemotherapeutics often fail to reach all diseased cells. To help solve this problem, researchers are investigating novel drug delivery systems. Liposomes are an attractive option due to their low toxicity, high biocompatibility, and potential to carry a large amount of a drug to the tumor site, all while avoiding being eliminated from the body. This study evaluates the penetration of doxorubicin-encased liposomes into three-dimensional cell cultures, or spheroids. Liposomes composed of lipids containing head groups of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol were created by extrusion...
July 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28730955/itraq-based-proteomic-analysis-of-appsw-ind-mice-provides-insights-into-the-early-changes-in-alzheimer-s-disease
#3
Nan Li, Pinghong Hu, Tiantian Xu, Huan Chen, Xiaoying Chen, Jianwen Hu, Xifei Yang, Lei Shi, Jian-Hong Luo, Junyu Xu
BACKGROUND: Several proteins have been identified as potential diagnostic biomarkers in imaging, genetic, or proteomic studies in Alzheimer disease (AD) patients and mouse models. However, biomarkers for presymptom diagnosis of AD are still under investigation, as are the presymptom molecular changes in AD pathogenesis. OBJECTIVE: In this study, we aim to analyzed the early proteomic changes in APPSw,Ind mice and to conduct further functional studies on interesting proteins...
July 19, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28730643/differences-in-gadolinium-retention-after-repeated-injections-of-macrocyclic-mr-contrast-agents-to-rats
#4
Simona Bussi, Alessandra Coppo, Catherine Botteron, Valérie Fraimbault, Antonello Fanizzi, Elisa De Laurentiis, Sonia Colombo Serra, Miles A Kirchin, Fabio Tedoldi, Federico Maisano
PURPOSE: To compare the levels of gadolinium in the blood, cerebrum, cerebellum, liver, femur, kidneys, and skin after multiple exposure of rats to the macrocyclic gadolinium-based contrast agents (GBCAs) gadoterate, gadobutrol, and gadoteridol. MATERIALS AND METHODS: Fifty male Wistar Han rats were randomized to three exposure groups (n = 15 per group) and one control group (n = 5). Animals in the exposure groups received a total of 20 GBCA administrations (four administrations per week for 5 consecutive weeks) at a dose of 0...
July 21, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28730308/tlc-surface-integrity-affects-the-detection-of-alkali-adduct-ions-in-tlc-maldi-analysis
#5
Yonghui Dong, Ruggero Ferrazza, Andrea Anesi, Graziano Guella, Pietro Franceschi
Direct coupling of thin-layer chromatography (TLC) with matrix-assisted laser desorption ionization (MALDI) mass spectrometry allows fast and detailed characterization of a large variety of analytes. The use of this technique, however, presents great challenges in semiquantitative applications because of the complex phenomena occurring at the TLC surface. In our laboratory, we recently observed that the ion intensities of several alkali adduct ions were significantly different between the top and interior layer of the TLC plate...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28727443/plasmonic-thermal-decomposition-digestion-of-proteins-a-rapid-on-surface-protein-digestion-technique-for-mass-spectrometry-imaging
#6
Rong Zhou, Franco Basile
A method based on plasmon surface resonance absorption and heating was developed to perform a rapid on-surface protein thermal decomposition and digestion suitable for imaging MS and/or profiling. This photo-thermal process or plasmonic thermal decomposition/digestion (plasmonic-TDD) method incorporates a continuous wave (CW) laser excitation and gold nanoparticles (Au-NPs) to induce known thermal decomposition reactions that cleave peptides and proteins specifically at the C-terminus of aspartic acid and at the N-terminus of cysteine...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727418/combining-tof-sims-imaging-mass-spectrometry-and-cars-microspectroscopy-reveals-lipid-patterns-reminiscent-of-gene-expression-patterns-in-the-wing-imaginal-disc-of-drosophila-melanogaster
#7
Florian Marty, Gianluca Rago, Donald F Smith, Xiaoli Gao, Gert B Eijkel, Luke MacAleese, Mischa Bonn, Erich Brunner, Konrad Basler, Ron M A Heeren
Using label-free ToF-SIMS imaging mass spectrometry, we generated a map of small molecules differentially expressed in the Drosophila wing imaginal disc. The distributions of these moieties were in line with gene expression patterns observed during wing imaginal disc development. Combining ToF-SIMS imaging and coherent anti-Stokes Raman spectroscopy (CARS) microspectroscopy allowed us to locally identify acylglycerols as the main constituents of the pattern differentiating the future body wall tissue from the wing blade tissue...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28727417/oxygen-dependent-lipid-profiles-of-3d-cultured-human-chondrocytes-revealed-by-maldi-msi
#8
Brenda Bakker, Gert B Eijkel, Ron M A Heeren, Marcel Karperien, Janine N Post, Berta Cillero Pastor
Articular cartilage is exposed to a gradient of oxygen levels ranging from 5% at the surface to 1% in the deepest layers. While most cartilage research is performed in supraphysiological oxygen levels (19-21%), culturing chondrocytes under hypoxic oxygen levels (≤ 8%) promotes the chondrogenic phenotype. Exposure of cells to various oxygen levels alters their lipid metabolism, but detailed studies examining how hypoxia affects lipid metabolism in chondrocytes are lacking. To better understand the chondrocyte's behavior in response to oxygen we cultured 3D pellets of human primary chondrocytes in normoxia (20% oxygen) and hypoxia (2...
July 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28726323/characterization-of-a-cathepsin-d-protease-from-cho-cell-free-medium-and-mitigation-of-its-impact-on-the-stability-of-a-recombinant-therapeutic-protein
#9
Amareth Lim, Brandon L Doyle, Gerard M Kelly, Angelia M Reed-Bogan, Lawrence H Breen, Parviz A Shamlou, Peter K Lambooy
During purification process development of a recombinant therapeutic protein, an endoproteolytic activity endogenous to the Chinese hamster ovary (CHO) cells and leading to degradation at particular hydrophobic amino acid residues (e.g., Phe and Trp) was observed when processing at acidic pH. The presence of residual levels of protease activity in purified protein batches affected the inherent activity of the product when stored as a solution. In order to develop a robust purification strategy to minimize this undesirable impact, identification and characterization of this protease was essential to ultimately ensure that a solution formulation was stable for many years...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28725224/nanobody-technology-a-versatile-toolkit-for-microscopic-imaging-protein-protein-interaction-analysis-and-protein-function-exploration
#10
REVIEW
Els Beghein, Jan Gettemans
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor their use over conventional antibodies or fragments thereof, in selected areas of research. In this review, we assess the current status of nanobodies as research tools in diverse aspects of fundamental research. We discuss the use of nanobodies as detection reagents in fluorescence microscopy and focus on recent advances in super-resolution microscopy...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28721672/ir-maldesi-mass-spectrometry-imaging-at-50-micron-spatial-resolution
#11
Mark T Bokhart, Jeffrey Manni, Kenneth P Garrard, Måns Ekelöf, Milad Nazari, David C Muddiman
High spatial resolution in mass spectrometry imaging (MSI) is crucial to understanding the biology dictated by molecular distributions in complex tissue systems. Here, we present MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 μm resolution. An adjustable iris, beam expander, and an aspherical focusing lens were used to reduce tissue ablation diameters for MSI at high resolution. The laser beam caustic was modeled using laser ablation paper to calculate relevant laser beam characteristics...
July 18, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28718648/multi-imaging-of-cytokinin-and-abscisic-acid-on-the-roots-of-rice-oryza-sativa-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#12
Katsuhiro Shiono, Riho Hashizaki, Toyofumi Nakanishi, Tatsuko Sakai, Takushi Yamamoto, Koretsugu Ogata, Ken-Ichi Harada, Hajime Ohtani, Hajime Katano, Shu Taira
Plant hormones are important mobile endogenous signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Visual information on more than one plant hormone from the same sample would be especially powerful, because it could enable rapid, intuitive interpretation. However, multi-imaging techniques for plant hormones have been unavailable. Here, we report the first multi-imaging of two plant hormones, one of cytokinin (i.e. trans-zeatin) and abscisic acid (ABA) by MALDI-TOF-MS imaging...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28717220/imaging-mass-spectrometry-and-ms-ms-molecular-networking-reveals-chemical-interactions-among-cuticular-bacteria-and-pathogenic-fungi-associated-with-fungus-growing-ants
#13
Cristopher A Boya P, Hermógenes Fernández-Marín, Luis C Mejía, Carmenza Spadafora, Pieter C Dorrestein, Marcelino Gutiérrez
The fungus-growing ant-microbe symbiosis is an ideal system to study chemistry-based microbial interactions due to the wealth of microbial interactions described, and the lack of information on the molecules involved therein. In this study, we employed a combination of MALDI imaging mass spectrometry (MALDI-IMS) and MS/MS molecular networking to study chemistry-based microbial interactions in this system. MALDI IMS was used to visualize the distribution of antimicrobials at the inhibition zone between bacteria associated to the ant Acromyrmex echinatior and the fungal pathogen Escovopsis sp...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28714776/tissue-localization-of-glycosphingolipid-accumulation-in-a-gaucher-disease-mouse-brain-by-lc-esi-ms-ms-and-high-resolution-maldi-imaging-mass-spectrometry
#14
E Ellen Jones, Wujuan Zhang, Xueheng Zhao, Cristine Quiason, Stephanie Dale, Sheerin Shahidi-Latham, Gregory A Grabowski, Kenneth D R Setchell, Richard R Drake, Ying Sun
To better understand regional brain glycosphingolipid (GSL) accumulation in Gaucher disease (GD) and its relationship to neuropathology, a feasibility study using mass spectrometry and immunohistochemistry was conducted using brains derived from a GD mouse model (4L/PS/NA) homozygous for a mutant GCase (V394L [4L]) and expressing a prosaposin hypomorphic (PS-NA) transgene. Whole brains from GD and control animals were collected using one hemisphere for MALDI FTICR IMS analysis and the other for quantitation by LC-ESI-MS/MS...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28712653/neural-circuit-specialized-astrocytes-transcriptomic-proteomic-morphological-and-functional-evidence
#15
Hua Chai, Blanca Diaz-Castro, Eiji Shigetomi, Emma Monte, J Christopher Octeau, Xinzhu Yu, Whitaker Cohn, Pradeep S Rajendran, Thomas M Vondriska, Julian P Whitelegge, Giovanni Coppola, Baljit S Khakh
Astrocytes are ubiquitous in the brain and are widely held to be largely identical. However, this view has not been fully tested, and the possibility that astrocytes are neural circuit specialized remains largely unexplored. Here, we used multiple integrated approaches, including RNA sequencing (RNA-seq), mass spectrometry, electrophysiology, immunohistochemistry, serial block-face-scanning electron microscopy, morphological reconstructions, pharmacogenetics, and diffusible dye, calcium, and glutamate imaging, to directly compare adult striatal and hippocampal astrocytes under identical conditions...
July 11, 2017: Neuron
https://www.readbyqxmd.com/read/28712591/normalizing-ovulation-rate-by-preferential-reduction-of-hepato-visceral-fat-in-adolescent-girls-with-polycystic-ovary-syndrome
#16
Lourdes Ibáñez, Luis Del Río, Marta Díaz, Giorgia Sebastiani, Óscar J Pozo, Abel López-Bermejo, Francis de Zegher
PURPOSE: Polycystic ovary syndrome (PCOS) is an increasingly prevalent disorder in adolescent girls, commonly presenting with hirsutism/oligomenorrhea, commonly treated with an oral contraceptive (OC), and commonly followed by oligoanovulatory subfertility. We tested whether an intervention targeting the reduction of hepato-visceral adiposity is followed by a higher ovulation rate than OC treatment. METHODS: This randomized, open-label, single-center, pilot proof-of-concept study (12 months on treatment, then 12 months off) was performed in adolescent girls with hirsutism and oligomenorrhea (PCOS by National Institutes of Health; no sexual activity; N = 36; mean age 16 years, body mass index 23...
July 13, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/28709739/structured-lifestyle-intervention-in-patients-with-the-metabolic-syndrome-mitigates-oxidative-stress-but-fails-to-improve-measures-of-cardiovascular-autonomic-neuropathy
#17
Subramaniam Pennathur, Mamta Jaiswal, Elizabeth A White, Lynn Ang, David M Raffel, Melvyn Rubenfire, Rodica Pop-Busui
AIMS: To assess the role of oxidative stress in mediating adverse outcomes in metabolic syndrome (MetS) and resultant cardiovascular autonomic neuropathy (CAN), and to evaluate the effects of lifestyle interventions on measures of oxidative stress and CAN in subjects with MetS. METHODS: Pilot study in 25 non-diabetic subjects with MetS (age 49±10years, 76% females) participating in a 24-week lifestyle intervention (supervised aerobic exercise/Mediterranean diet), and 25 age-matched healthy controls...
May 2, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28707960/biofunctionalized-hybrid-magnetic-gold-nanoparticles-as-catalysts-for-photothermal-ablation-of-colorectal-liver-metastases
#18
Sarah B White, Dong-Hyun Kim, Yang Guo, Weiguo Li, Yihe Yang, Jeane Chen, Venkateswara R Gogineni, Andrew C Larson
Purpose To demonstrate that anti-MG1 conjugated hybrid magnetic gold nanoparticles (HNPs) act as a catalyst during photothermal ablation (PTA) of colorectal liver metastases, and thus increase ablation zones. Materials and Methods All experiments were performed with approval of the institutional animal care and use committee. Therapeutic and diagnostic multifunctional HNPs conjugated with anti-MG1 monoclonal antibodies were synthesized, and the coupling efficiency was determined. Livers of 19 Wistar rats were implanted with 5 × 10(6) rat colorectal liver metastasis cell line cells...
July 13, 2017: Radiology
https://www.readbyqxmd.com/read/28704559/synthesis-cytotoxicity-and-antitumour-mechanism-investigations-of-polyoxometalate-doped-silica-nanospheres-on-breast-cancer-mcf-7-cells
#19
Hongqian Cao, Chunyan Li, Wen Qi, Xiangjun Meng, Rui Tian, Yanfei Qi, Wei Yang, Juan Li
Polyoxometalates (POMs) have shown the potential anti-bacterial, anti-viral and anti-tumor activities. In order to improve their physiological stability and antitumour activity for medical application, K2Na[AsIIIMo6O21(O2CCH2NH3)3]·6H2O doped silica nanospheres (POM@SiO2) with diameters of ~40 nm have been synthesized by the water-in-oil microemulsion method in this study. The obtained spheres were morphologically uniform nanosized and nearly monodispersed in solution. The nanoparticles had high entrapment efficiency, which was upto 46...
2017: PloS One
https://www.readbyqxmd.com/read/28702764/the-direct-analysis-of-drug-distribution-of-rotigotine-loaded-microspheres-from-tissue-sections-by-lesa-coupled-with-tandem-mass-spectrometry
#20
Li-Xiao Xu, Tian-Tian Wang, Yin-Yin Geng, Wen-Yan Wang, Yin Li, Xiao-Kun Duan, Bin Xu, Charles C Liu, Wan-Hui Liu
The direct analysis of drug distribution of rotigotine-loaded microspheres (RoMS) from tissue sections by liquid extraction surface analysis (LESA) coupled with tandem mass spectrometry (MS/MS) was demonstrated. The RoMS distribution in rat tissues assessed by the ambient LESA-MS/MS approach without extensive or tedious sample pretreatment was compared with that obtained by a conventional liquid chromatography tandem mass spectrometry (LC-MS/MS) method in which organ excision and subsequent solvent extraction were commonly employed before analysis...
July 12, 2017: Analytical and Bioanalytical Chemistry
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