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Mass Spectrometery

Aparupa Sengupta, Dibyendu Sarkar, Padmini Das, Saumik Panja, Chinmayi Parikh, Dilrukshi Ramanathan, Susan Bagley, Rupali Datta
Environmental contamination by antibiotics not only perturbs the ecological balance but also poses a risk to human health by promoting the development of multiantibiotic-resistant bacteria. This study focuses on identifying the biochemical pathways associated with tetracycline (TC) transformation/degradation in vetiver grass that has the potential to be used as a biological remediation system in TC-contaminated water sources. A hydroponic experimental setup was used with four initial TC concentrations (0, 5, 35, 75 ppm), and TC uptake was monitored over a 30-day period...
September 23, 2016: Environmental Science and Pollution Research International
Nicole A Hofmann, Jiang Yang, Sunia A Trauger, Hironao Nakayama, Lan Huang, Dirk Strunk, Marsha A Moses, Michael Klagsbrun, Joyce Bischoff, Wolfgang F Graier
BACKGROUND AND PURPOSE: Highly vascularized ovarian carcinoma secretes the putative endocannabinoid and GPR55 agonist, L-α-lysophosphatidylinositol (LPI), into the circulation. We aimed to assess the involvement of this agonist and its receptor in ovarian cancer angiogenesis. EXPERIMENTAL APPROACH: Secretion of LPI by three ovarian cancer cell lines (OVCAR-3, OVCAR-5 and COV-362) was tested by mass spectrometry. Involvement of cancer cell-derived LPI on angiogenesis was tested in the in vivo chicken chorioallantoic membrane (CAM) assay along with the assessment of the effect of LPI on proliferation, network formation, and migration of neonatal and adult human endothelial colony-forming cells (ECFCs)...
August 2015: British Journal of Pharmacology
Maryam Lashgari, Chanbasha Basheer, Hian Kee Lee
In the present study, micro-solid phase extraction (µ-SPE) followed by liquid chromatography-triple tandem mass spectrometery (LC-MS/MS) was developed for the determination of perfluorocarboxylic acids (PFCAs) at trace levels in water samples. The µ-SPE device comprised of a porous polypropylene membrane bag containing 5mg sorbent. The membrane bag acted as a clean-up filter and prevented matrix compounds from interfering with the extraction process. Analysis was carried out by LC-MS/MS in negative electrospray ionization mode...
August 15, 2015: Talanta
Ryan R Otter, David McKinney, Bobby Brown, Susan Lainer, William Monroe, Don Hubbs, Bob Read
On December 22, 2008, a dike containing coal fly ash at the Tennessee Valley Authority Kingston Fossil Plant (TN, USA) failed, and within months, dredging operations began to remove ash-contaminated sediments. The purpose of this study was to investigate differences in the bioaccumulation of metals in three mussel species during and after dredging operations. Mussels were caged for approximately 1 year during dredging and after, and then mussel condition index values and As, Cd, Cr, Pb, Ni, Se, Hg, U, Fe, Mg, Al, Sb, Ba, Be, Co, Cu, Mn, Mo, Ag, Sr, Tl, V, and Zn concentrations in soft tissue were determined via inductively coupled plasma-mass spectrometery...
June 2015: Environmental Monitoring and Assessment
Wei Liu, Qin-Wen Zou, Xian-Long Cheng, Ping Zhang, Yan Shi, Feng Wei, Shu Zhang, Li-Jun Lun, De-Quan Yu
OBJECTIVE: To control the quality of Xihuangwan by improving the qualitative detection of artificial musk inside this pill. METHOD: The qualitative detection of artificial musk was carried out by gas chromatography-mass spectrometery(GC-MS). RESULT: The established GC-MS successfully detected artificial musk in Xihuangwan. CONCLUSION: GC-MS is reliable,accurate,and practical in the identification of artificial must inside Xihuangwan...
December 2014: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
E Szałek, A Karbownik, D Murawa, K Połom, A Tezyk, J Gracz, T Grabowski, E Grześkowiak, A Biczysko-Murawa, P Murawa
OBJECTIVE: Oxycodone is a semi-synthetic opioid with a stronger analgesic effect than morphine and codeine. The efficacy of this opioid in the treatment of postoperative pain has been proved in different groups of patients. The drug has a favourable adverse reaction profile, which encourages doctors and patients to use it more and more widely. The drug is also used in the patients who underwent an abdominal surgery, e.g. stomach resection. Gastrectomy leads to pathophysiological changes within the gastrointestinal tract, which may cause changes in the drug absorption...
October 2014: European Review for Medical and Pharmacological Sciences
Seung-Ah Lee, Hongfeng Jiang, Chad M Trent, Jason J Yuen, Sureshbabu Narayanasamy, Robert W Curley, Earl H Harrison, Ira J Goldberg, Mathew S Maurer, William S Blaner
Dietary carotenoids like β-carotene are converted within the body either to retinoid, via β-carotene-15,15'-dioxygenase (BCO1), or to β-apo-carotenoids, via β-carotene-9',10'-oxygenase 2. Some β-apo-carotenoids are potent antagonists of retinoic acid receptor (RAR)-mediated transcriptional regulation, which is required to ensure normal heart development and functions. We established liquid chromatography tandem mass spectrometery methods for measuring concentrations of 10 β-apo-carotenoids in mouse plasma, liver, and heart and assessed how these are influenced by Bco1 deficiency and β-carotene intake...
December 1, 2014: American Journal of Physiology. Heart and Circulatory Physiology
Yong-Hyun Kim, Ki-Hyun Kim
Accurate values for the Henry's law constants are essential to describe the environmental dynamics of a solute, but substantial errors are recognized in many reported data due to practical difficulties in measuring solubility and/or vapor pressure. Despite such awareness, validation of experimental approaches has scarcely been made. An experimental approach based on thermal desorption-gas chromatography-mass spectrometery (TD-GC-MS) method was developed to concurrently allow the accurate determination of target compounds from the headspace and aqueous samples in closed equilibrated system...
May 16, 2014: Journal of Chromatography. A
David J Askenazi, John F Moore, Naomi Fineberg, Rajesh Koralkar, Stephanie Clevenger, Jon Daniel Sharer
BACKGROUND: Measurement of serum creatinine (SCr) and urine creatinine (UCr) is regularly used in clinical and research settings. For small animal experiments and for studies in which sample collection is spare (i.e. neonatal cohorts), measuring SCr and UCr using tiny amounts of sample (as low as 10 mcl) would maximize exploration and minimize iatrogenic blood loss. METHODS: We performed an evaluation in six healthy adults to determine differences between SCr and UCr values in different methodologies and storage environments and time...
September 2014: Journal of Clinical Laboratory Analysis
J C Wallwork
Methods for the measurement of the zinc content of blood components (plasma, erythrocytes, platelets, mononuclear leukocytes, and polymorphonuclear neutrophils) as indicators of the zinc status of animals have been reviewed and evaluated. The values of plasma or serum zinc concentrations as indicators of zinc status in experimental animals or humans is questionable. Consequently, the zinc content of blood cellular components has been suggested as better indicators of zinc status. Methodological problems, such as incomplete cellular separation or zinc contamination, occur in some of the procedures applied to the quantitation of blood cellular component zinc...
April 1987: Biological Trace Element Research
W V Ligon, H Grade
The use of a mixture of 5% ammonia in methane, where the ammonia is 50% (15)N-labeled, provides a very useful reagent gas for chemical ionization mass spectrometry. We find that this combination gives spectra very much like pure ammonia reagent gas except that all of the adduct ions are clearly labeled.
June 1994: Journal of the American Society for Mass Spectrometry
Patrick M Antle, Christian D Zeigler, Yuriy Gankin, Albert Robbat
New mass spectral deconvolution algorithms have been developed for comprehensive two-dimensional gas chromatography/quadrupole mass spectrometry (GC × GC/qMS). This paper reports the first use of spectral deconvolution of full scan quadrupole GC × GC/MS data for the quantitative analysis of polycyclic aromatic hydrocarbons (PAH) and polycyclic aromatic sulfur heterocycles (PASH) in coal tar-contaminated soil. A method employing four ions per isomer and multiple fragmentation patterns per alkylated homologue (MFPPH) is used to quantify target compounds...
November 5, 2013: Analytical Chemistry
Ellen Hondrogiannis, Andrew Schmidt, Frank Iannaconi, Erin Ehrlinger
Inductively coupled plasma-mass spectrometery (ICP-MS) was used to quantitate elemental impurities in sulfamide from seven common vendors, Acros, Aldrich, Alfa-Aesar, Fluka (Lot #1 and Lot #2), Maybridge, Pfaltz and Bauer, and Spectrum (Lot #1 and Lot #2) with the goal of using these impurities to differentiate the sulfamide samples and link the sulfamide back to its vendor. This attribution of the sulfamide back to its vendor is forensically relevant since sulfamide is a precursor to the deadly neurotoxin, tetramethylenedisulfotetramine (TETS)...
October 10, 2013: Forensic Science International
Danyang Wu, Stephen E Duirk
The goal of this study was to develop a robust method capable of quantifying taste and odor compounds (i.e., geosmin and 2-methylisoborneol) at very low aqueous concentrations in the presence of wastewater and algal derived contaminants. A polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber was used to perform headspace-solid phase microextraction (HS-SPME) to extract and analyze taste and odor compounds from model, source water, and finished drinking water samples. Gas chromatography coupled with mass spectrometery (GC/MS) in full scan mode was used to analyze the compounds desorbed from the fiber in the GC inlet...
June 2013: Chemosphere
Yoram Cohen, Nitza Goldenberg-Cohen, Sharon Akrish, Tali Shani, Ninette Amariglio, Olga Dratviman-Storobinsky, Ilana Kaplan, Iris Barshack, Abraham Hirshberg
OBJECTIVE: The genetic factors participating in oral melanoma evolution have not been studied extensively. We aimed to analyze the prevalence of BRAF and GNAQ mutations in a series of oral melanocytic tumors, nevi, and melanomas. STUDY DESIGN: The study group consisted of 4 melanomas and 10 nevi (6 intramucosal, 4 blue nevi). DNA was extracted from paraffin-embedded tissue sections, and mutations in GNAQ and BRAF were analyzed with the use of mass spectrometery...
December 2012: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Juanjuan Chen, Tingting Yan, Jilin Xu, Shan He, Peng Zhao, Xiaojun Yan
A facile method based on liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry working in selected reaction monitoring mode has been established to analyze toxins in the algae and water samples. Twelve types of toxins (anatoxin, cylindrospermopsin, dinophysistoxin-1, nodularin, okadaic acid, microcystins) were efficiently separated under optimized liquid chromatography coupled with mass spectrometry conditions in the selected reaction monitoring mode. Correlation coefficients of the calibration curves, all felt in the range of 0...
May 2012: Journal of Separation Science
Bhavna Chawla, Anupam Jhingran, Aswini Panigrahi, Kenneth D Stuart, Rentala Madhubala
Leishmania donovani is a protozoan parasite that causes visceral leishmaniasis (VL) and is responsible for significant mortality and morbidity. Increasing resistance towards antimonial drugs poses a great challenge in chemotherapy of VL. Paromomycin is an aminoglycosidic antibiotic and is one of the drugs currently being used in the chemotherapy of cutaneous and visceral leishmaniasis. To understand the mode of action of this antibiotic at the molecular level, we have investigated the global proteome differences between the wild type AG83 strain and a paromomycin resistant (PRr) strain of L...
2011: PloS One
Neha Biyani, Alok Kumar Singh, Swati Mandal, Bhavna Chawla, Rentala Madhubala
Visceral Leishmaniasis (VL) is a parasitic disease caused by the protozoan parasite Leishmania donovani. Resistance to pentavalent antimonials (SbV), the mainstay therapy for leishmaniasis is now a major concern, due to emergence of drug resistance. Hence, understanding the underlying mechanism of resistance to antimonials is required. Here we used quantitative mass spectrometery to identify global proteome differences between antimony-susceptible/-resistant isolates. We detected modification of expression of proteins involved in the key metabolic pathways...
October 2011: Molecular and Biochemical Parasitology
Frances Reed, Rachel Burrow, Katrien L C Poels, Lode Godderis, Hendrik A F Veulemans, Martina Mosing
OBJECTIVE: To investigate whether the method used to attach matrix-type fentanyl patches influences the degree of skin attachment and the amount of active drug remaining in patches after use. STUDY DESIGN: Prospective, randomised clinical study. STUDY POPULATION: Fifteen adult dogs of mixed breeds. METHODS: Two equally sized matrix-type fentanyl patches were attached to the dorsal third of the lateral thorax of fifteen dogs for 72 hours...
July 2011: Veterinary Anaesthesia and Analgesia
Ajay Rana, Devendra Dhyani, Ashu Gulati, Harsh Pratap Singh
Two major iridoid glucosides, namely plantarenaloside, a neurotrophic compound, and boschnaloside, an antibacterial iridoid glucoside, have been isolated from the shoots of Incarvillea emodi (a wild ornamental plant). The compounds are characterised by NMR spectral data and ESI mass spectrometery. This is the first ever report on the isolation of iridoid glucosides from I. emodi (Bignoniaceae).
June 2011: Natural Product Research
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