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Component resolved diagnostics

Manik Lal Saha, Xuzhou Yan, Peter J Stang
Over the past couple of decades, coordination-driven self-assembly has evolved as a broad multidisciplinary domain that not only covers the syntheses of aesthetically pleasing supramolecular architectures but also emerges as a method to form new optical materials, chemical sensors, theranostic agents, and compounds with light-harvesting and emissive properties. The majority of these applications depend upon investigations that reveal the photophysical nature and electronic structure of supramolecular coordination complexes (SCCs), including two-dimensional (2D) metallacycles and three-dimensional (3D) metallacages...
October 13, 2016: Accounts of Chemical Research
Claudia I Henschke, Rowena Yip, James P Smith, Andrea S Wolf, Raja M Flores, Mingzhu Liang, Mary M Salvatore, Ying Liu, Dong Ming Xu, David F Yankelevitz
OBJECTIVE: The purpose of this study was to assess the frequencies of identifying participants with part-solid nodules, of diagnostic pursuit, of diagnoses of lung cancer, and long-term lung cancer survival in baseline and annual repeat rounds of CT screening in the International Early Lung Cancer Action Project. MATERIALS AND METHODS: Screenings were performed under a common protocol. Participants with solid, nonsolid, and part-solid nodules and the diagnoses of lung cancer were documented...
October 11, 2016: AJR. American Journal of Roentgenology
L Lange, L Lasota, A Finger, D Vlajnic, S Büsing, J Meister, I Brockaert, C Pfannenstiel, F Friedrichs, M Price, V Trendelenburg, B Niggemann, K Beyer
INTRODUCTION: Component resolved diagnostics using specific IgE to 2 S albumins has shown to be a valuable new option in diagnostic procedure. Ana o 3 is a 2 S albumin from cashew. Aim of this study was to investigate the role of Ana o 3 specific serum IgE in the diagnosis of cashew allergy and to identify cut-off levels to replace oral food challenges. Moreover, the value of additional determination of total IgE has been investigated. METHODS: In a multicentre study we analysed specific IgE to cashew extract and Ana o 3 as well as total IgE in children with suspected cashew allergy using the ImunoCAP-FEIA and a standardized diagnostic procedure including oral challenges where indicated...
September 19, 2016: Allergy
Magnus P Borres, Nobuyuki Maruyama, Sakura Sato, Motohiro Ebisawa
Due to the high prevalence of food allergic diseases globally there are increasing demands in clinical practice for managing IgE-mediated conditions. During the last decade, component resolved diagnostics has been introduced into the field of clinical allergology, providing information that cannot be obtained from extract-based tests. Component resolved data facilitate more precise diagnosis of allergic diseases and identify sensitizations attributable to cross-reactivity. Furthermore it assists risk assessment in clinical practice as sensitization to some allergenic molecules is related to persistence of clinical symptoms and systemic rather than local reactions...
October 2016: Allergology International: Official Journal of the Japanese Society of Allergology
Małgorzata Leśniak, Maciej Juda, Łukasz Dyczek, Maria Czarnobilska, Magdalena Leśniak, Ewa Czarnobilska
Food allergy is most often linked to the type I allergic reaction, while IgE-dependent mechanism causes symptoms in only about 50% of patients. If symptoms are coming from other types of allergic reactions we do not have enough standardized diagnostic methods. The purpose of our review is to discuss the possibilities of diagnosis of food allergies. Regardless of the causal mechanism the interview has the most important role in the diagnosis, and the gold standard is a double blind placebo controlled food challenge...
2016: Przegla̧d Lekarski
Johanna Schmidt, Sven Peters, Lydia Sauer, Dietrich Schweitzer, Matthias Klemm, Regine Augsten, Nicolle Müller, Martin Hammer
PURPOSE: To discriminate non-proliferative diabetic retinopathy (NPDR) patients from healthy controls by fluorescence lifetime imaging ophthalmoscopy (FLIO). METHODS: A prototype FLIO (Heidelberg-Engineering, Heidelberg, Germany) was used to examine the retina of 33 patients and 28 controls. As increased fluorescence of the diabetic lens is known, the lenses of 34 patients and 24 controls were investigated as well. Time-resolved decay was detected in two spectral channels (ch1: 498-560 nm, ch2: 560-720 nm) and approximated by a series of three exponential functions yielding in lifetimes (τ1 , τ2 , τ3 ), amplitudes (α1 , α2 , α3 ) and their amplitude-weighted means (τm )...
August 13, 2016: Acta Ophthalmologica
Nicolas M Orsi, Mini Menon
Primary ovarian carcinoid tumors are exceptionally rare entities accounting for approximately 0.1% of all ovarian neoplasms. This report describes a primary ovarian neuroendocrine tumor arising in association with a mature cystic teratoma in a 65 year-old woman. Macroscopically, the unilateral adnexal tumor was composed of cystic, solid and mucinous elements which resolved into a dual component lesion histologically. The majority of the tumor displayed an organoid architecture with mild to moderate pleomorphism and no discernible mitotic activity, while approximately 10% consisted of sheets and groups of cells with highly pleomorphic nuclei, necrosis and occasional mitoses...
August 2016: Gynecologic Oncology Reports
E González-Mancebo, J Domínguez-Ortega, S Blanco-Bermejo, E González-Seco, M J Trujillo, F de la Torre
BACKGROUND: Over the last years, different works have been published about the importance of incorporating new diagnosis techniques in allergic patients such as component-resolved diagnosis (CRD). The objective of this study is to compare the evolution of allergic sensitisation profiles by means of CRD and cutaneous tests (SPT) on pollen-allergic patients. METHODS: A total of 123 patients aged between 2 and 14 years were included in an open, prospective, multicentre study...
July 28, 2016: Allergologia et Immunopathologia
M B Dreval, A M Shapoval, F I Ozherelyev, M M Makhov
An Hα camera has been designed and installed in the U-3M torsatron for spatially and temporally resolved measurements. This device provides fast measurements of the emission brightness profile in the noisy environment of the radio frequency (RF) heated plasma. Unusual topology of diagnostics and the data acquisition system are applied. All the system components, including digitizers, are assembled in a single unit. It allows the suppression of a low-frequency electromagnetic interference by eliminating the ground loops...
July 2016: Review of Scientific Instruments
Alicia Armentia, Eugenia Sanchís, Javier A Montero
PURPOSE OF REVIEW: Conventional diagnostic tests in allergy are insufficient to clarify the cause of vernal conjunctivitis. Component-resolved diagnostic (CRD) by microarray allergen assay may be useful in detecting allergens that might be involved in the inflammatory process. RECENT FINDINGS: In a recent trial in patients suffered from eosinophilic esophagitis, after 2 years of the CRD-guided exclusion diet and specific immunotherapy, significant clinical improvement was observed, and 68% of patients were discharged (cure based on negative biopsy, no symptoms, and no medication intake)...
October 2016: Current Opinion in Allergy and Clinical Immunology
Nicola Fuiano, Cristoforo Incorvaia
INTRODUCTION: The diagnostic work-up of allergic rhinitis (AR) is first and foremost based on the combination of clinical history data and results of skin prick tests (SPT). Other tests, including specific IgE measurement, nasal challenge, and, as a third option, component resolved diagnosis or basophil activation test, may be useful when the diagnosis is difficult because of polysensitization or when negative results of SPT are observed despite a suggestive history for allergy. The atopy patch test (APT) that assesses the type 4 delayed hypersensitivity allergy is currently not sufficiently used...
May 2016: Iranian Journal of Otorhinolaryngology
Matthew Mcgarry, Ronny Li, Iason Apostolakis, Pierre Nauleau, Elisa E Konofagou
The mechanical properties of arteries are implicated in a wide variety of cardiovascular diseases, many of which are expected to involve a strong spatial variation in properties that can be depicted by diagnostic imaging. A pulse wave inverse problem (PWIP) is presented, which can produce spatially resolved estimates of vessel compliance from ultrasound measurements of the vessel wall displacements. The 1D equations governing pulse wave propagation in a flexible tube are parameterized by the spatially varying properties, discrete cosine transform components of the inlet pressure boundary conditions, viscous loss constant and a resistance outlet boundary condition...
August 7, 2016: Physics in Medicine and Biology
Nieves Segura, Teresa Abos, José A Compaired, Esther Compés, Isabel Guallar, Manuel Morales, Susana Monzón, José Mozota, Pilar Muñoz, Jesús Pola, Macarena Quintana, Beatriz Rojas, Sara San Juan, Felicitas Villa, Cristina Zapata, Lucía Jimeno, Fernando de la Torre
BACKGROUND: Profilin sensitisation is considered a diagnostic confounding factor in areas where patients are exposed to multiple pollens. The aim of this study is to assess pollen sensitisation profiles in adults and children and to evaluate, by means of component-resolved diagnosis (CRD) and skin prick testing (SPT), which pollens may be considered as risk factors of profilin sensitisation in order to establish the best diagnostic approach in polysensitised patients. METHODS: A total of 231 pollen-allergic patients (adults and children) were included, out of the pollen season, from an area with similar levels of pollen exposure...
2016: Clinical and Translational Allergy
Philip R Cohen, Kenneth S Arfa
BACKGROUND: Ferrofluid is a colloidal suspension that usually consists of surfactant-coated nanoparticles of magnetite (Fe3O4) in a carrier liquid. Ferromagnetic fluid forms spikes when the liquid is exposed to a magnetic field. PURPOSE: The authors describe a man who developed temporary discoloration of his right palm and fingers after accidental cutaneous contact with ferrofluid and discuss some of the current and potential applications of this unique liquid. METHODS: A 28-year-old man was evaluating the effects of magnetic fields using ferrofluid...
March 2016: Journal of Clinical and Aesthetic Dermatology
Carina Venter, Kate Maslin, Syed Hasan Arshad, Veeresh Patil, Jane Grundy, Gillian Glasbey, Roger Twiselton, Taraneh Dean
BACKGROUND: Patients often report adverse reactions to wheat. Interpretation of sensitization to wheat pollen and flour with/without sensitization to grass pollen is a clinical problem. AIM: We set out to determine the prevalence of wheat allergy in a birth cohort (10/11 year olds) and investigate the usefulness of performing skin prick tests (SPT), specific IgE tests and component resolved diagnostics to wheat pollen and flour. METHODS: The Food Allergy and Intolerance Research (FAIR) birth cohort included babies born on the Isle of Wight (UK) between September 2001-August 2002 (n = 969)...
2016: Clinical and Translational Allergy
F C van Erp, R J B Klemans, Y Meijer, C K van der Ent, A C Knulst
Instead of relying on crude peanut extract, component-resolved diagnostics (CRD) uses sensitization to allergenic proteins within peanut. In this review, we describe the recent advances and future perspectives of the use of CRD in the management of peanut-allergic patients. There is strong evidence that sensitization to Ara h 2 is the best predictor for clinically relevant peanut allergy in children and adults. Isolated sensitization to other peanut components is only rarely present in patients with systemic reactions to peanut...
2016: Current Treatment Options in Allergy
Leonieke N van Veen, Michiel Heron, Manou Batstra, Paul M M van Haard, Hans de Groot
BACKGROUND: To date, diagnosing food allergies in children still presents a diagnostic dilemma, leading to uncertainty concerning the definite diagnosis of peanut allergy, as well as to the need for strict diets and the potential need for adrenalin auto-injectors. This uncertainty in particular is thought to contribute to a lower quality of life. In the diagnostic process double-blind food challenges are considered the gold standard, but they are time-consuming as well as potentially hazardous...
2016: BMC Pediatrics
Xiaoqin Zhong, Liang Qiao, Natalia Gasilova, Baohong Liu, Hubert H Girault
A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepared by coating AuNPs with individual allergenic proteins and mass barcode, represented by polyethylene glycol molecules of various chain lengths. Magnetic beads (MBs) functionalized with antihuman IgE antibodies (Abs) were used as immunomagnetic capture probes...
June 21, 2016: Analytical Chemistry
Lina Zhu, Tiantian She, Yingying Zhang, Shaoshen Li, Zixian Xu, Juanjuan Yan, Huiqiang Li
SCOPE: Crab is a major source of shellfish allergens. Most studies have focused on allergens in crab muscle (CM) rather than on allergens in crab ovary (CO). This study aimed to identify potential allergens in CO from Eriocheir sinensis. METHODS AND RESULTS: Dot blot and immunoblotting results revealed the differential reactivity of CM and CO extracts to positive sera from patients allergic to crabs. Four CO proteins showed good specific IgE-binding activities in 2-DE/immunoblotting analysis; mass spectrometry identified the proteins as hemocyanin, vitellogenin, ovary development-related protein EJO1and EJO2...
May 31, 2016: Molecular Nutrition & Food Research
Kirsten Beyer, Bodo Niggemann
IgE-mediated immediate type reactions are the most common form of food allergy in childhood. Primary (often in early childhood) and secondary (often pollen-associated) allergies can be distinguished by their level of severity. Hen's egg, cow's milk and peanut are the most common elicitors of primary food allergy. Tolerance development in hen's egg and cow's milk allergy happens frequently whereas peanut allergy tends toward a lifelong disease. For the diagnostic patient history, detection of sensitization and (in many cases) oral food challenges are necessary...
June 2016: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
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