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Ling-Bing Meng, Ruomei Qi, Lei Xu, Yuhui Chen, Zemou Yu, Peng Guo, Tao Gong
BACKGROUND: The mortality of atherosclerotic cerebrovascular disease is on the rise, and changes in intimal and media thickness are a leading cause of cerebral ischemia-related death. Levels of low density lipoprotein cholesterol (LDLC), total cholesterol (TC), and chronic stress (CS) are all recognized risk factors for atherosclerosis (AS). However, the leading independent risk factor is indistinct. This study explored the effects of chronic stress, LDLC, and TC on AS and intimal and media thickness, preliminarily explored the main risk factor of AS, and analyzed the related histocyte mechanisms for macrophages and endothelial cells...
June 19, 2018: Lipids in Health and Disease
Shu Pan, Xingchen Liu, Hongcan Shi
Objective: To investigate the biocompatibility and immunogenicity of the tracheal matrix decellularized by sodium perchlorate (NaClO 4 ). Methods: Bone marrow mesenchymal stem cells (BMSCs) were divided from 2-month-old New Zealand white rabbits. The trachea of 6-month-old New Zealand white rabbits were trimmed to a length of 1.5 cm and randomly divided into control group (group A 1 , n =5, just stripped the loose connective tissue outside the trachea) and experimental group (group B 1 , n =5, decellularized by improved NaClO 4 immersion method)...
April 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Juan Li, Wu Yao, Jian Yong Hou, Lin Zhang, Lei Bao, Hui Ting Chen, Di Wang, Zhong Zheng Yue, Yi Ping Li, Miao Zhang, Chang Fu Hao
OBJECTIVE: The aim of this study was to investigate the effects of SiO2 on fibrocytes and whether fibrocytes participate in silicosis in vivo. METHODS: A macrophagocyte (AM)/fibrocyte coculture system was established, and AMs were treated with 100 μg/mL SiO2. Flow cytometry was used to detect the number of fibrocytes. Real-time PCR was performed to measure the expression of collagen I, collagen III, and α-SMA mRNA. The levels of collagen I, collagen III, and TGF-β1 protein were determined by ELISA...
September 2017: Biomedical and Environmental Sciences: BES
Zhengyu Ma, Yi Wu, Jing Wang, Changsheng Liu
We present P(TMC-co-DLLA) copolymer with the molar ratio of TMC: DLLA = 15: 85 was used to systematic study of in vivo and in vitro degradation behaviors. Dense homogeneous copolymer specimens were prepared by compression molding method. The in vitro and in vivo degradation were, respectively, performed at simulative body condition and implanted into rat's subcutaneous condition. Investigations were followed via physicochemical and histological analysis such as SEM, GPC, DSC, FTIR and H&E stain. The results demonstrate that copolymeric material can degrade in phosphate buffer solution (PBS) and in rat's body, and the in vivo degradation rate is faster...
August 2017: Regenerative Biomaterials
Qingling Jiang, Wenbo Chen, Yingxue Qin, Lixing Huang, Xiaojin Xu, Lingmin Zhao, Qingpi Yan
The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. hydrophila survival in the macrophagocytes, we used the mini-Tn10 transposon mutagenesis system to build a mutant library by mixing Escherichia coli Sm10 (pLOFKm) and A. hydrophila B11. In total, 102 mutant colonies were detected, and 11 of them showed reduced survival in macrophagocytes. The mutant with the most severe phenotype, AM73, was chosen for further research...
August 2017: MicrobiologyOpen
Jing Chen, Xiaoli Tian, Zijie Mei, Yacheng Wang, Ye Yao, Shimin Zhang, Xin Li, Hui Wang, Junhong Zhang, Conghua Xie
CpG-oligodeoxynucleotide (CpG-ODN) is not only reported to protect against airway hyper responsiveness but is also known as a potent vaccine adjuvant for anti-tumor therapy. Little is known about the effect of CpG-ODN in mice with radiation-induced lung fibrosis (RILF), a common late stage form of tissue damage that occurs after thorax radiotherapy (RT). Here, we evaluated the immunomodulatory effects of CpG-ODN on the development of RILF. Mice were divided into four groups: (1) RT, single dose of 12Gy to the whole thorax; (2) CpG, only intraperitoneal injection of CpG-ODN for total 5 weeks; (3) RT+CpG, irradiation plus CpG-ODN treatment before and after irradiation for total 5 weeks; and (4) control (CTL): No RT or CpG-ODN treatment...
December 2016: Molecular Immunology
Xiao-Yan Cai, Xiao-Chun Ni, Yong Yi, Hong-Wei He, Jia-Xing Wang, Yi-Peng Fu, Jian Sun, Jian Zhou, Yun-Feng Cheng, Jian-Jun Jin, Jia Fan, Shuang-Jian Qiu
Nucleoside triphosphate diphosphohydrolase-1 (ENTPD1/CD39) is the rate-limiting enzyme in a cascade leading to the generation of immunosuppressive adenosine and plays an important role in tumor progression. This study aimed to evaluate the expression of CD39 and CD39Foxp3 regulatory T cells (Tregs) and to determine their prognostic role in patients with hepatocellular carcinoma (HCC) after radical resection.Immunohistochemistry (IHC) and double IHC were used to analyze CD39 expression or the expression of CD39 and Foxp3 in a cohort of 324 HCC patients who underwent curative resection...
October 2016: Medicine (Baltimore)
E Y Choi, S S Lee, J Y Hyeon, S H Choe, B R Keum, J M Lim, D C Park, I S Choi, K K Cho
This research analyzed the effect of β-glucan that is expected to alleviate the production of the inflammatory mediator in macrophagocytes, which are processed by the lipopolysaccharide (LPS) of Escherichia. The incubated layer was used for a nitric oxide (NO) analysis. The DNA-binding activation of the small unit of nuclear factor-κB was measured using the enzyme-linked immunosorbent assay-based kit. In the RAW264.7 cells that were vitalized by Escherichia coli (E. coli) LPS, the β-glucan inhibited both the combatant and rendering phases of the inducible NO synthase (iNOS)-derived NO...
November 2016: Asian-Australasian Journal of Animal Sciences
C Y Liu, M Y Zheng, R Fu, H Q Wang, T Wang, W W Qi, Z H Shao
OBJECTIVE: To clarify the specific target of severe aplastic anemia (SAA) immune attack via identifying the target cells of cytotoxic T cell attacks and the expression of apoptosis ligand on each department and each stage of bone marrow hematopoietic cells. METHODS: A total of 15 SAA patients and 15 normal controls were recruited in the Department of Hematology, Tianjin Medical University General Hospital between March 2011 and March 2012. Factor associated suicide(Fas) protein expression of CD34(+) , CD14(+) , CD33(+) , and GlycoA(+) cells in bone marrow was detected by flow cytometry...
June 14, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Yanhong Li, Jigang Zhang, Yanfeng Xu, Yunlin Han, Binbin Jiang, Lan Huang, Hua Zhu, Yuhuan Xu, Weiling Yang, Chuan Qin
The biological effects of different wavelengths of light emitting diode (LED) light tend to vary from each other. Research into use of photobiomodulation for treatment of skin wounds and the underlying mechanisms has been largely lacking. We explored the histopathological basis of the therapeutic effect of photobiomodulation and the relation between duration of exposure and photobiomodulation effect of different wavelengths of LED in a Japanese big-ear white rabbit skin-wound model. Skin wound model was established in 16 rabbits (three wounds per rabbit: one served as control, the other two wounds were irradiated by red and blue LED lights, respectively)...
2016: PloS One
Wenxia Fan, Fenghua Qian, Qinlong Ma, Pei Zhang, Tingting Chen, Chunhai Chen, Yong Zhang, Ping Deng, Zhou Zhou, Zhengping Yu
OBJECTIVE: To investigate the effects of extremely low frequency electromagnetic field (ELF-EMF) on the proliferation and cytokine production of mesenchymal stem cells (MSC) and the effects of mesenchymal stem cell conditioned medium (MSC-CM) on the proliferation and migration of macrophagocytes (RAW264.7). METHODS: Bone marrow derived-mesenchymal stem cells (rBMSC) were isolated from rats, cultured and randomly divided into two groups: SHAM group (absence of electromagnetic field exposure) and EMF group...
2015: International Journal of Clinical and Experimental Medicine
Hongli Yu, Yaozong Pan, Hao Wu, Xiuyun Ge, Qian Zhang, Fagen Zhu, Baochang Cai
UNLABELLED: The present study aimed at investigating the alum-processing mechanism attenuating toxicity of Araceae Pinellia ternata and Pinellia pedatisecta. Animal retroperitoneal inflammatory model in vivo and macrophagocyte release inflammatory factor model in vitro were used to detect the effect of alum processing on raphides and lectin. Scanning electron microscopy was used to observe the change in raphides during processing; HPLC method was used to determine the correlation between the dissolution and corrosion of raphides and ion in the alum solution; (27)Al-NMR technology was used to detect the relationship between aluminum oxalate complex formation and the dissolved and corrosion of raphides...
October 2015: Archives of Pharmacal Research
Ting-Ting Ma, Bi-Tao Wu, Yan Lin, Hai-Yu Xiong, Qin Wang, Zi-Wei Li, Feng Cheng, Zhi-Guang Tu
Interleukin-12 (IL-12), a member of interleukin family, plays a critical role in immune responses and anti-tumor activity. In this study, the effects of IL-12 on monocytic tumor cell lines differentiation to macrophagocyte and its likely mechanism was investigated. We examined the differentiation markers, morphological and functional changes, and possible mechanism in IL-12-treated THP-1 and U937 cells. It was found that IL-12 could up-regulated macrophage surface marker CD68 and CD11b expression in a time-dependent manner...
April 2015: Molecular and Cellular Biochemistry
Yanxia Fu, Hailing Zhou, Mengqi Wang, Juren Cen, Qun Wei
Isogarcinol is a natural compound that we extracted from Garcinia mangostana L., and we were the first to report that it is a new immunosuppressant. In the present study, we investigated the immune regulation and anti-inflammatory effects of isogarcinol on collagen-induced arthritis (CIA) and explored its potential mechanism in the treatment of rheumatoid arthritis. The oral administration of isogarcinol significantly reduced clinical scores, alleviated cartilage and bone erosion, and reduced the levels of serum inflammatory cytokines in CIA mice...
May 7, 2014: Journal of Agricultural and Food Chemistry
V A Ameliushkina, S Zh Urazalina, T I Kotkina, S I Kaba, V N Titov
The clinical and pathomorphologic data demonstrate that the most frequent cause of cardiac infarction is the formation of "soft" atheromatosis plaques in the intima of arteries. Their rupture results in thrombosis of coronary arteries. The plaques are characterized by higher content of triglycerides. On the basis of the research data, it is possible to validly consider that the detection of secretary phospholipase content A2 conjugated with lipoproteins is the test of systemic inflammatory response. This response is formed under atherosclerosis in vivo as a feedback to the accumulation in the intercellular medium of the endogenic flogogens (initiators of biological reaction of inflammation)--lipoproteins of lower density subclass A...
March 2012: Klinicheskaia Laboratornaia Diagnostika
Stefan Prokop, Frank L Heppner, Hans H Goebel, Werner Stenzel
The etiopathogenesis of sarcoidosis, a systemic granulomatous disease, still remains obscure. A multitude of organs have been described to be affected in systemic sarcoidosis. Skeletal muscles may also be affected, leading to myalgia and weakness. A workup of the specific immune response with emphasis on the macrophage response is provided herein. Affected muscle tissue from seven patients with systemic sarcoidosis was analyzed and compared with that from seven patients with other myopathies containing macrophagocytic infiltration...
March 2011: American Journal of Pathology
Yu Niu, Ai-Li He, Wang-Gang Zhang
Hemophagocytic lymphohistiocytosis (HLH) is named as hemophagocytic syndrome (HPS) and is a complicated disease with reactive hyperplasia of mononuclear/macrophagocytic system. This disease characterised by release of massive cytokines and severe functional destruction of visceral organs, which results from immune function disturbance causing by various pathogenic factors. The cardinal clinical symptoms of HLH are prolonged fever, hepatosplenomegaly, cytopenia, elevated ferritin and triglycerides, low fibrinogen, symptom in nerve system and so on...
February 2010: Zhongguo Shi Yan Xue Ye Xue za Zhi
H Noguchi
The pathological changes observed in the organs in Macacus rhesus monkeys which have succumbed to severe infection with Bartonella bacilliformis are similar to those found in human organs in persons dying of Oroya fever. The characteristic changes in the liver are the zonal necrosis of the cells around the hepatic veins, involving active macrophagocytosis of invading polymorphonuclear leucocytes in the necrotic areas, and a marked endothelial hyperplasia in the sinusoids or around the portal veins. In some instances there is fatty infiltration of hepatic cells...
February 28, 1927: Journal of Experimental Medicine
Yong-Qing Zhang, Gao-Sheng Huang, Xie-Qun Chen, Qing-Xian Bai, Rong Liang, Zhe Wang
This study was purposed to investigate the changes of bcl-6 expression in K562 cells and the mechanism inducing differentiation into different myelocyte lineages. Models of K562 cells inducing differentiation to lineages of megakaryocyte, erythrocyte and macrophagocyte were established with inducers TPA (tetradecanoylphorbol 13-acetate), Hu (hydroxyurea) and HMBA (hexamethylene bisacetamide) respectively. Western blot assay was applied to detect the expression of bcl-6 in K562 cells before and after the induction...
April 2009: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shu-feng Yang, Jian-ning Zhao
Aseptic loosening is one of the most frequent long-term complications after joint replacement,which limits the service life of prostheses. A lot of studies have been focused on interface membranes around prostheses recently. In interface membranes, there are plenty of macrophagocytes and desmocytes,which play vital roles in osteolysis. This study gave a review of interface membrane including its formation, cellulosity, osteolysis factor, immunity reaction and turnover. The advances of interface membranes will help us to comprehend the pathogenesy and treatment of the aseptic loosening...
April 2008: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
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