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assisted reproduction techniques

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April 24, 2018: Journal of Maternal-fetal & Neonatal Medicine
Carlos Balmori, Elisa Varela
PURPOSE OF REVIEW: The purpose of this review is to analyze what is known to date about the relation between telomeres and male fertility, and if it is possible for telomeres, or elements related to them, to be used as new prognostic biomarkers in fertility treatment. RECENT FINDINGS: Cells in germ series, including spermatozoids, have longer telomeres (10-20 kb), and do not seem to undergo the shortening that takes place in somatic cells with age as they present telomerase activity...
April 16, 2018: Current Opinion in Obstetrics & Gynecology
Bibhas Kar, Subbiah Sivamani, Shankar Kundavi, Thankam Rama Varma
Objective: The present study outlines three cases of a Robertsonian translocation and the consequences for the initiation of pregnancy by intracytoplasmic sperm injection (ICSI). Three case histories are presented documenting structural chromosome abnormalities in infertile males. Materials and Methods: Semen analysis was performed according to the World Health Organization guidelines. Chromosome analysis was performed using G-banding. Y chromosome microdeletions were detected by multiplex polymerase chain reaction assays...
April 2018: Journal of Obstetrics and Gynaecology of India
Erin Wingo, Natalie Ingraham, Sarah C M Roberts
BACKGROUND: Little research documents the self-identified reproductive health priorities and health care experiences of lesbian, gay, bisexual, transgender, queer (LGBTQ)-identified individuals who may be in need of services. METHODS: We conducted in-depth interviews with a diverse sample of 39 female-assigned-at-birth individuals (ages 18-44) who also identified as lesbian, bisexual, queer, and/or genderqueer, or transmasculine. Interviews were primarily conducted in person in the Bay Area of California, and Baltimore, Maryland, with 11 conducted remotely with participants in other U...
April 13, 2018: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
F Jiménez-Trejo, I Coronado-Mares, M Boeta, I González-Santoyo, R Vigueras-Villaseñor, C Arriaga-Canon, L Herrera-Montalvo, M Tapia-Rodríguez
Assisted reproductive techniques have been used on several domestic animals to preserve desirable traits in strains of high genetic and commercial value; however in equines its efficiency rate is relatively low. To increase the conception ratio in stallions, some research groups have used pharmacological treatments which promote sperm hyperactivation in order to increase male's fertility rates. In this way, our previous work suggests that serotonin (5-HT) could be a good pharmacological candidate that facilitates conception rate in domestic horses...
April 16, 2018: Histology and Histopathology
Aime K Johnson
The demand for feline semen collection, evaluation, and subsequent use is growing as a way to preserve important genetic materials. This article describes semen collection methods using a variety of techniques that can be applicable in almost any setting. Also discussed are cryopreservation methods that optimize sperm survival.
April 12, 2018: Veterinary Clinics of North America. Small Animal Practice
Aime K Johnson
Assisted reproduction in the queen can range from simple ovulation induction to more advanced techniques such as in vitro fertilization. This article describes techniques available and the success associated with each.
April 12, 2018: Veterinary Clinics of North America. Small Animal Practice
Mauro Bibancos De Rose, Mariana Moraes Piccolomini
Assisted reproduction techniques (ART) have significantly advanced since the first successful in vitro fertilization (IVF). However, most in vitro-produced embryos fail to implant. Key steps in ART are the correct infertility diagnosis, in order to manage the individual treatments, and the assessment of gamete and embryo viability, to identify the embryo with the best implantation potential. Non-invasive approaches to correctly diagnose infertility and to access embryo development potential have the advantages of increasing the knowledge of embryo physiology, therefore allowing the development of methods to predict developmental competence and viability...
April 12, 2018: Protein and Peptide Letters
Ada Soler-Ventura, Judit Castillo, Alberto de la Iglesia, Meritxell Jodar, Ferran Barrachina, Josep Lluis Ballesca, Rafael Oliva
Protamines are the most abundant sperm nuclear proteins and pack approximately the 92-98% of the mammalian sperm DNA. In mammals, two types of protamines have been described, the protamine 1 (P1) and the protamine 2 (P2) family, whose relative ratio has been related to the proper sperm chromatin maturity and the normality/abnormality of the male gamete. Specifically, the deregulation of the P1/P2 ratio has been correlated to DNA damage, alterations in seminal parameters, and low success rate of assisted reproduction techniques...
April 12, 2018: Protein and Peptide Letters
Xianghu Meng, Longchang Fan, Tao Wang, Shaogang Wang, Zengjun Wang, Jihong Liu
Background: Psychogenic anejaculation (PAE) is a rare disease in clinic seriously affecting quality of life, especially fertility of patients. However, there is not an effective treatment for these patients. The aim of the study was to evaluate the results of treatment with electroejaculation (EEJ), intrauterine insemination (IUI), and intracytoplasmic sperm injection (ICSI) in patients with PAE who were refractory to penile vibratory stimulation (PVS). Methods: Twenty PVS-refractory patients with PAE were included in the retrospective study...
March 2018: Translational Andrology and Urology
P Boyer, F Lefebvre, C Siraudin, D Montjean, P Le Coz, M Gervoise-Boyer
OBJECTIVES: We organized a survey for in-vitro fertilization couples who beneficiated on self-preservation of part of the oocyte cohort. The main objective was to measure couples' satisfaction. Secondary objectives were; to identify how patients had been informed; to verify that the use of the ART technique met their expectations, and how they felt about oocyte or embryo freezing. METHODS: The data were collected by a questionnaire sent electronically to couples who had undergone partial vitrification of the oocyte cohort and at least one warming cycle...
April 2, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
Seda Karabulut, Özlem Aksünger, Can Ata, Yusuf Sağıroglu, İlknur Keskin
Fertilization problems are the major problems that may be faced in 30-55% of the patients during an intracytoplasmic sperm injection (ICSI) cycle. A successful oocyte activation depends on factors related to both sperm and oocyte, and one of the important factors that mediates the process is Ca2+ concentration within the oocyte. Artificial oocyte activation (AOA) is a method used for fertilization problems that commonly involve the usage of Ca2+ ionophores and is usually used in problems such as total fertilization failure (TFF) and globozoospermia...
April 5, 2018: Systems Biology in Reproductive Medicine
Asghar Beigi Harchegani, Hossein Shafaghatian, Eisa Tahmasbpour, Alireza Shahriary
MicroRNAs (miRNAs) are a novel class of small noncoding RNAs (ncRNAs) that play critical roles in regulation of gene expression, especially at posttranscriptional level. Over the past decade, the degree to which miRNAs are involved in male infertility has become clear. They are expressed in a cell- or phase-specific manner during spermatogenesis and play crucial role in male reproductive health. Therefore, dysregulation of miRNAs in testicular cells can be considered as a molecular basis for reproductive failure and male infertility...
January 1, 2018: Reproductive Sciences
M Viera-Molina, M D Guerra-Martín
There are a high number of people affected by infertility who require assisted reproduction techniques. For this reason the aim of this review was to analyze the effectiveness of the interventions carried out using these techniques. A systematic peer review was performed on twelve databases. Inclusion criteria were: randomized clinical trials, published between 2011 and 2016, and with a score greater than two on the Jadad scale. The pharmacological interventions that proved effective were the administration of hormones, intrauterine human chorionic gonadotropin, gonadotrophin and dehydroepiandrosterone, and metformin...
March 26, 2018: Anales del Sistema Sanitario de Navarra
Cuiling Lu, Yaoyao Zhang, Xiaoying Zheng, Xueling Song, Ruiyang, Jieyan, Huailiang Feng, Jie Qiao
In vitro maturation (IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years. Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016...
March 19, 2018: Science China. Life Sciences
Kyeong Han Kim, Soobin Jang, Ju Ah Lee, Ho-Yeon Go, Jeeyoun Jung, Sunju Park, Myeong Soo Lee, Seong-Gyu Ko
INTRODUCTION: Infertility has long been recognized as a treatable disease, and complementary and alternative medicine treatments, such as acupuncture and moxibustion, have been used in Korea and China. This study describes female infertility treatment programs that used traditional Korean medicine (TKM) and were conducted by local governments in Korea and evaluates its effectiveness and safety. METHODS: The authors officially requested related information from the report of the infertility treatment programs and related sources from 2006 to 2016 from the Health & Welfare Ministry of Korea and the Association of Korean Medicine (AKOM)...
March 22, 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
Maria Portia B Nagata, Kenji Endo, Kazuko Ogata, Kenichi Yamanaka, Junki Egashira, Naoto Katafuchi, Tadayuki Yamanouchi, Hideo Matsuda, Yuki Goto, Miki Sakatani, Takuo Hojo, Hirofumi Nishizono, Kenji Yotsushima, Naoki Takenouchi, Yutaka Hashiyada, Kenichi Yamashita
Selection of functional spermatozoa plays a crucial role in assisted reproduction. Passage of spermatozoa through the female reproductive tract requires progressive motility to locate the oocyte. This preferential ability to reach the fertilization site confers fertility advantage to spermatozoa. Current routine sperm selection techniques are inadequate and fail to provide conclusive evidence on the sperm characteristics that may affect fertilization. We therefore developed a selection strategy for functional and progressively motile bovine spermatozoa with high DNA integrity based on the ability to cross laminar flow streamlines in a diffuser-type microfluidic sperm sorter (DMSS)...
March 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
Y S Tian, J J Zhang, Z T Li, J Tang, M L Cheng, Y P Wu, W H Ma, Z F Pang, W S Li, J M Zhai, B Li
Embryo cryopreservation is important for long-term preservation of germplasm and assisted reproduction. However, it is still very difficult to obtain viable embryos from cryopreserved fish embryos. In this study, embryos of Epinephelus moara were used to investigate the effects of various cryopreservation methods. Embryos in stages 10 pairs somite (10S), 18 pairs somite (18S), 22 pairs somite (22S), tail-bud (TB), embryo twitching (ET) and pre-hatch (PH) were treated with five-step equilibrium penetration in 40% PMG3T vitrification solution, which contained 15...
March 12, 2018: Theriogenology
Charalampos S Siristatidis, Eleni Sertedaki, Dennis Vaidakis, Christos Varounis, Marialena Trivella
BACKGROUND: In order to overcome the low effectiveness of assisted reproductive technologies (ART) and the high incidence of multiple births, metabolomics is proposed as a non-invasive method to assess oocyte quality, embryo viability, and endometrial receptivity, and facilitate a targeted subfertility treatment. OBJECTIVES: To evaluate the effectiveness and safety of metabolomic assessment of oocyte quality, embryo viability, and endometrial receptivity for improving live birth or ongoing pregnancy rates in women undergoing ART, compared to conventional methods of assessment...
March 16, 2018: Cochrane Database of Systematic Reviews
Robert John Aitken
This article is a personal perspective on male infertility, a condition that is not only extremely prevalent but also a major reason for couples to resort to assisted reproductive technology. The introduction of intra-cytoplasmic sperm injection (ICSI) as a form of facilitated fertilization had a revolutionary impact on our capacity to treat cases of male infertility associated with severely-compromised semen quality. However, the widespread use of this technique is also thought to pose risks in terms of the incidence of miscarriage, the health and wellbeing of the offspring and perpetuation of the infertile phenotype into future generations...
March 13, 2018: Molecular Human Reproduction
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