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Bone morphogenic protein 1

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https://www.readbyqxmd.com/read/28389839/p15-peptide-stimulates-chondrogenic-commitment-and-endochondral-ossification
#1
Jun Zhang, Peter Eisenhauer, Ozҫan Kaya, Alexander R Vaccaro, Carol Diallo, Andrzej Fertala, Theresa A Freeman
PURPOSE: The synthetic 15 amino acid biomimetic peptide sequence (P15) derived from a region of the alpha (α)-1 chain of collagen I, has been shown to promote α2 integrin activation and enhance intramembranous ossification. In this study, we ask if the P15 peptide also enhances bone formation through endochondral ossification, and determine if direct binding of α2 integrin with P15 mediates integrin activation. METHODS: Mesenchymal cells (C3H10T1/2) were cultured in chondrogenic media and the expression of chondrogenic markers and integrin activation was determined by Western blot and fluorescent immunohistochemistry...
April 8, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28363788/mesenchymal-stem-cell-fate-following-non-viral-gene-transfection-strongly-depends-on-the-choice-of-delivery-vector
#2
T Gonzalez-Fernandez, B N Sathy, C Hobbs, G M Cunniffe, H O McCarthy, N J Dunne, V Nicolosi, F J O'Brien, D J Kelly
Controlling the phenotype of mesenchymal stem cells (MSCs) through the delivery of regulatory genes is a promising strategy in tissue engineering (TE). Essential to effective gene delivery is the choice of gene carrier. Non-viral delivery vectors have been extensively used in TE, however their intrinsic effects on MSC differentiation remain poorly understood. The objective of this study was to investigate the influence of three different classes of non-viral gene delivery vectors: (1) cationic polymers (polyethylenimine, PEI), (2) inorganic nanoparticles (nanohydroxyapatite, nHA) and (3) amphipathic peptides (RALA peptide) on modulating stem cell fate after reporter and therapeutic gene delivery...
March 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28344026/the-impact-of-various-scaffold-components-on-vascularized-bone-constructs
#3
Ahmad Eweida, Matthias Schulte, Oliver Frisch, Ulrich Kneser, Leila Harhaus
Bone tissue engineering is gaining more interest in the field of craniofacial surgery where continuous efforts are being made to improve the outcomes via modulation of the scaffold components. In an in vitro three dimensional (3D) culture, the effect of bone morphogenic protein 2 (BMP2, 60 μg/ml) and the effect of different cell seeding densities (0.25, 0.5, and 1 × 104) of rat mesenchymal stem cells seeded on nanocrystalline hydroxyapatite in silica gel matrix (Nanobone(®)) on the cell viability and differentiation were studied...
February 24, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28331138/delayed-expression-of-circulating-tgf-%C3%AE-1-and-bmp-2-levels-in-human-nonunion-long-bone-fracture-healing
#4
Yoshiaki Hara, Mohammad Ghazizadeh, Hajime Shimizu, Hisashi Matsumoto, Nobuyuki Saito, Takanori Yagi, Kazuki Mashiko, Kunihiro Mashiko, Makoto Kawai, Hiroyuki Yokota
BACKGROUND: The healing process of bone fracture requires a well-controlled multistage and sequential order beginning immediately after the injury. However, complications leading to nonunion exist, creating serious problems and costs for patients. Transforming growth factor-beta 1 (TGF-β1) and bone morphogenic protein 2 (BMP-2) are two major growth factors involved in human bone fracture healing by promoting various stages of bone ossification. In this study, we aimed to determine the role of these factors during the fracture healing of human long bones and assess their impacts on nonunion condition...
2017: Journal of Nippon Medical School, Nippon Ika Daigaku Zasshi
https://www.readbyqxmd.com/read/28276640/prospective-review-of-mesenchymal-stem-cells-differentiation-into-osteoblasts
#5
REVIEW
Priyanka Garg, Matthew M Mazur, Amy C Buck, Meghan E Wandtke, Jiayong Liu, Nabil A Ebraheim
Stem cell research has been a popular topic in the past few decades. This review aims to discuss factors that help regulate, induce, and enhance mesenchymal stem cell (MSC) differentiation into osteoblasts for bone regeneration. The factors analyzed include bone morphogenic protein (BMP), transforming growth factor β (TGF-β), stromal cell-derived factor 1 (SDF-1), insulin-like growth factor type 1 (IGF-1), histone demethylase JMJD3, cyclin dependent kinase 1 (CDK1), fucoidan, Runx2 transcription factor, and TAZ transcriptional coactivator...
February 2017: Orthopaedic Surgery
https://www.readbyqxmd.com/read/28236164/evaluation-of-the-effects-of-the-combination-of-autologous-mesenchymal-stem-cells-and-platelet-rich-plasma-on-structural-bone-allograft-healing
#6
Chang-Goo Park, Min Wook Joo, Jinyoung Jeong, Yong-Koo Kang, Da-Reum Lee
In this study, we evaluated the efficacy of the combination of autologous mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) for enhancing structural allogenic bone graft healing in rabbits. For these experiments segmental bone defects (1.5-2 cm) were generated on femoral diaphysis of New Zealand white rabbits and reconstructed via structural allogenic bone grafting and additional intramedullary nail fixation. The structural allografts were subsequently wrapped with Gelfoam containing autologous MSCs and PRP, or PRP alone (control)...
February 24, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28214837/efficacy-of-glucosamine-sulphate-in-skin-ageing-results-from-an-ex-vivo-anti-ageing-model-and-a-clinical-trial
#7
Audrey Gueniche, Isabelle Castiel-Higounenc
BACKGROUND: Glucosamine sulphate (GS) is essential in the biosynthesis of glycolipids, glycoproteins, glycosaminoglycans (GAGs), hyaluronate, and proteoglycans. Connective tissues primarily contain collagen and proteoglycans and play an important role in skin ageing. OBJECTIVE: The objectives were to assess ex vivo the impact of GS on skin ageing parameters and in vivo the effect of GS on the skin physiology of mature healthy volunteers after oral intake. METHODS: The impact of GS on skin ageing was assessed ex vivo via different immunohistochemical assays and histology and via a clinical study using biopsies...
2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28214472/bmp-12-activates-tenogenic-pathway-in-human-adipose-stem-cells-and-affects-their-immunomodulatory-and-secretory-properties
#8
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Agnieszka Kulesza, Kamila Gala, Beata Kaleta, Katarzyna Zielniok, Katarzyna Siennicka, Marek Sabat, Leszek Paczek
BACKGROUND: Cell-based therapy is a treatment method in tendon injuries. Bone morphogenic protein 12 (BMP-12) possesses tenogenic activity and was proposed as a differentiating factor for stem cells directed to transplantation. However, BMPs belong to pleiotropic TGF-β superfamily and have diverse effect on cells. Therefore, the aim of this study was to determine if BMP-12 induces tenogenic differentiation of human adipose stem cells (hASCs) and how it affects other features of this population...
February 18, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28159972/role-of-id-proteins-in-bmp4-inhibition-of-profibrotic-effects-of-tgf-%C3%AE-2-in-human-tm-cells
#9
Avani A Mody, Robert J Wordinger, Abbot F Clark
Purpose: Increased expression of TGF-β2 in primary open-angle glaucoma (POAG) aqueous humor (AH) and trabecular meshwork (TM) causes deposition of extracellular matrix (ECM) in the TM and elevated IOP. Bone morphogenetic proteins (BMPs) regulate TGF-β2-induced ECM production. The underlying mechanism for BMP4 inhibition of TGF-β2-induced fibrosis remains undetermined. Bone morphogenic protein 4 induces inhibitor of DNA binding proteins (ID1, ID3), which suppress transcription factor activities to regulate gene expression...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28145426/two-novel-bmp-2-variants-identified-in-patients-with-thoracic-ossification-of-the-ligamentum-flavum
#10
Xiaochen Qu, Zhongqiang Chen, Dongwei Fan, Shen Xiang, Chuiguo Sun, Yan Zeng, Weishi Li, Zhaoqing Guo, Qiang Qi, Woquan Zhong, Yun Jiang
Thoracic ossification of the ligamentum flavum (TOLF)is a common cause of thoracic spinal canal stenosis and has been reported almost exclusively in East Asian countries. In this study, we established a relationship between bone morphogenic protein 2 (BMP-2) and TOLF. We divided patients into two groups according to severity of ossification and identified susceptible loci through exome sequencing. We identified 39 novel likely pathogenic variants in 29 genes in the transforming growth factor-beta (TGF-β) superfamily or TGF-β/BMPs signaling pathway, including two missense variants in BMP-2 (NM_001200...
May 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28128866/cyclin-dependent-kinase-inhibitor-1-deficient-mice-are-susceptible-to-osteoarthritis-associated-with-enhanced-inflammation
#11
Shinsuke Kihara, Shinya Hayashi, Shingo Hashimoto, Noriyuki Kanzaki, Koji Takayama, Tomoyuki Matsumoto, Nobuaki Chinzei, Kenjiro Iwasa, Masahiko Haneda, Kazuhiro Takeuchi, Kotaro Nishida, Ryosuke Kuroda
Osteoarthritis (OA) is a multifactorial disease, and recent data suggested that cell cycle-related proteins play a role in OA pathology. Cyclin-dependent kinase (CDK) inhibitor 1 (p21) regulates activation of other CDKs, and recently, we reported that p21 deficiency induced susceptibility to OA induced by destabilization of the medial meniscus (DMM) surgery through STAT3-signaling activation. However, the mechanisms associated with why p21 deficiency led to susceptibility to OA by the STAT3 pathway remain unknown...
January 27, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28101231/the-biological-significance-of-methylome-differences-in-human-papilloma-virus-associated-head-and-neck-cancer
#12
Maria J Worsham, Kang Mei Chen, Indrani Datta, Josena K Stephen, Dhananjay Chitale, Alexandra Gothard, George Divine
In recent years, studies have suggested that promoter methylation in human papilloma virus (HPV) positive head and neck squamous cell carcinoma (HNSCC) has a mechanistic role and has the potential to improve patient survival. The present study aimed to replicate key molecular findings from previous analyses of the methylomes of HPV positive and HPV negative HNSCC in an independent cohort, to assess the reliability of differentially methylated markers in HPV-associated tumors. HPV was measured using real-time quantitative PCR and the biological significance of methylation differences was assessed by Ingenuity Pathway Analysis (IPA)...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28035075/the-hippo-signaling-pathway-provides-novel-anti-cancer-drug-targets
#13
REVIEW
June Sung Bae, Sun Mi Kim, Ho Lee
The Hippo signaling pathway plays a crucial role in cell proliferation, apoptosis, differentiation, and development. Major effectors of the Hippo signaling pathway include the transcriptional co-activators Yes-associated protein 1 (YAP) and WW domain-containing transcription regulator protein 1 (TAZ). The transcriptional activities of YAP and TAZ are affected by interactions with proteins from many diverse signaling pathways as well as responses to the external environment. High YAP and TAZ activity has been observed in many cancer types, and functional dysregulation of Hippo signaling enhances the oncogenic properties of YAP and TAZ and promotes cancer development...
February 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28002369/transient-local-bone-remodeling-effects-of-rhbmp-2-in-an-ovine-interbody-spine-fusion-model
#14
Hyun W Bae, Vikas V Patel, Zeeshan M Sardar, Jeffrey M Badura, Ben B Pradhan, Howard B Seim, A Simon Turner, Jeffrey M Toth
BACKGROUND: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a powerful osteoinductive morphogen capable of stimulating the migration of mesenchymal stem cells (MSCs) to the site of implantation and inducing the proliferation and differentiation of these MSCs into osteoblasts. Vertebral end-plate and vertebral body resorption has been reported after interbody fusion with high doses of rhBMP-2. In this study, we investigated the effects of 2 rhBMP-2 doses on peri-implant bone resorption and bone remodeling at 7 time points in an end-plate-sparing ovine interbody fusion model...
December 21, 2016: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/27994786/bone-morphogenic-protein-is-a-viable-adjunct-for-fusion-in-minimally-invasive-transforaminal-lumbar-interbody-fusion
#15
M Mashfiqul Arafin Siddiqui, Ana Rosario P Sta Ana, William Yeo, Wai-Mun Yue
STUDY DESIGN: Comparison of prospectively collected data of patients undergoing minimally invasive surgery transforaminal lumbar interbody fusion (MIS-TLIF) with and without recombinant human bone morphogenic protein 2 (BMP). PURPOSE: To compare the clinical, radiological outcome and complications of patients undergoing MIS-TLIF with and without BMP. OVERVIEW OF LITERATURE: BMP is an effective fusion enhancer with potential complications. Direct comparison of MIS-TLIF with and without BMP is limited to retrospective studies with short follow-up...
December 2016: Asian Spine Journal
https://www.readbyqxmd.com/read/27943413/aberrant-connective-tissue-differentiation-towards-cartilage-and-bone-underlies-human-keloids-in-african-americans
#16
Judilyn Fuentes-Duculan, Kathleen M Bonifacio, Mayte Suárez-Fariñas, Norma Kunjravia, Sandra Garcet, Tristan Cruz, Claire Q F Wang, Hui Xu, Patricia Gilleadeau, Mary Sullivan-Whalen, Michael H Tirgan, James G Krueger
Keloids are benign fibroproliferative tumors more frequently found among African Americans. Until now, keloid etiopathogenesis is not fully understood. To characterize keloids in African Americans, we performed transcriptional profiling of biopsies from large chronic keloids, adjacent non-lesional (NL) skin (n=3) and a newly formed keloid lesion using Affymetrix HGU133 2.0 plus arrays. Quantitative RT-PCR (qRT-PCR) and immunohistochemistry (IHC) staining were performed to confirm increased expression of relevant genes...
December 11, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27911727/role-of-the-bone-morphogenic-protein-pathway-in-developmental-haemopoiesis-and-leukaemogenesis
#17
REVIEW
Parto Toofan, Helen Wheadon
Myeloid leukaemias share the common characteristics of being stem cell-derived clonal diseases, characterised by excessive proliferation of one or more myeloid lineage. Chronic myeloid leukaemia (CML) arises from a genetic alteration in a normal haemopoietic stem cell (HSC) giving rise to a leukaemic stem cell (LSC) within the bone marrow (BM) 'niche'. CML is characterised by the presence of the oncogenic tyrosine kinase fusion protein breakpoint cluster region-abelson murine leukaemia viral oncogene homolog 1 (BCR-ABL), which is responsible for driving the disease through activation of downstream signal transduction pathways...
October 15, 2016: Biochemical Society Transactions
https://www.readbyqxmd.com/read/27907211/characterization-and-immunomodulatory-effects-of-canine-adipose-tissue-and-bone-marrow-derived-mesenchymal-stromal-cells
#18
Keith A Russell, Natalie H C Chow, David Dukoff, Thomas W G Gibson, Jonathan LaMarre, Dean H Betts, Thomas G Koch
BACKGROUND: Mesenchymal stromal cells (MSC) hold promise for both cell replacement and immune modulation strategies owing to their progenitor and non-progenitor functions, respectively. Characterization of MSC from different sources is an important and necessary step before clinical use of these cells is widely adopted. Little is known about the biology and function of canine MSC compared to their mouse or human counterparts. This knowledge-gap impedes development of canine evidence-based MSC technologies...
2016: PloS One
https://www.readbyqxmd.com/read/27898568/whole-exome-sequencing-to-identify-novel-biological-pathways-associated-with-infertility-after-pelvic-inflammatory-disease
#19
Brandie D Taylor, Xiaojing Zheng, Toni Darville, Wujuan Zhong, Kranti Konganti, Olayinka Abiodun-Ojo, Roberta B Ness, Catherine M O'Connell, Catherine L Haggerty
BACKGROUND: Ideal management of sexually transmitted infections (STI) may require risk markers for pathology or vaccine development. Previously, we identified common genetic variants associated with chlamydial pelvic inflammatory disease (PID) and reduced fecundity. As this explains only a proportion of the long-term morbidity risk, we used whole-exome sequencing to identify biological pathways that may be associated with STI-related infertility. METHODS: We obtained stored DNA from 43 non-Hispanic black women with PID from the PID Evaluation and Clinical Health Study...
January 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27852784/activity-induces-fmr1-sensitive-synaptic-capture-of-anterograde-circulating-neuropeptide-vesicles
#20
Samantha L Cavolo, Dinara Bulgari, David L Deitcher, Edwin S Levitan
Synaptic neuropeptide and neurotrophin stores are maintained by constitutive bidirectional capture of dense-core vesicles (DCVs) as they circulate in and out of the nerve terminal. Activity increases DCV capture to rapidly replenish synaptic neuropeptide stores following release. However, it is not known whether this is due to enhanced bidirectional capture. Here experiments at the Drosophila neuromuscular junction, where DCVs contain neuropeptides and a bone morphogenic protein, show that activity-dependent replenishment of synaptic neuropeptides following release is evident after inhibiting the retrograde transport with the dynactin disruptor mycalolide B or photobleaching DCVs entering a synaptic bouton by retrograde transport...
November 16, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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