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https://www.readbyqxmd.com/read/28531327/interstrand-cross-links-arising-from-strand-breaks-at-true-abasic-sites-in-duplex-dna
#1
Zhiyu Yang, Nathan E Price, Kevin M Johnson, Yinsheng Wang, Kent S Gates
Interstrand cross-links are exceptionally bioactive DNA lesions. Endogenous generation of interstrand cross-links in genomic DNA may contribute to aging, neurodegeneration, and cancer. Abasic (Ap) sites are common lesions in genomic DNA that readily undergo spontaneous and amine-catalyzed strand cleavage reactions that generate a 2,3-didehydro-2,3-dideoxyribose sugar remnant (3'ddR5p) at the 3'-terminus of the strand break. Interestingly, this strand scission process leaves an electrophilic α,β-unsaturated aldehyde residue embedded within the resulting nicked duplex...
May 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28531158/impact-analysis-of-flow-shaping-in-ethernet-avb-tsn-and-afdx-from-network-calculus-and-simulation-perspective
#2
Feng He, Lin Zhao, Ershuai Li
Ethernet-AVB/TSN (Audio Video Bridging/Time-Sensitive Networking) and AFDX (Avionics Full DupleX switched Ethernet) are switched Ethernet technologies, which are both candidates for real-time communication in the context of transportation systems. AFDX implements a fixed priority scheduling strategy with two priority levels. Ethernet-AVB/TSN supports a similar fixed priority scheduling with an additional Credit-Based Shaper (CBS) mechanism. Besides, TSN can support time-triggered scheduling strategy. One direct effect of CBS mechanism is to increase the delay of its flows while decreasing the delay of other priority ones...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28531121/a-qpcr-based-tool-to-diagnose-the-presence-of-harmful-cyanobacteria-and-cyanotoxins-in-drinking-water-sources
#3
Yi-Ting Chiu, Yi-Hsuan Chen, Ting-Shaun Wang, Hung-Kai Yen, Tsair-Fuh Lin
Harmful cyanobacteria have been an important concern for drinking water quality for quite some time, as they may produce cyanotoxins and odorants. Microcystis and Cylindrospermopsis are two common harmful cyanobacterial genera detected in freshwater lakes and reservoirs, with microcystins (MCs) and cylindrospermopsin (CYN) as their important metabolites, respectively. In this study, two sets of duplex qPCR systems were developed, one for quantifying potentially-toxigenic Microcystis and Microcystis, and the other one for cylindrospermopsin-producing cyanobacteria and Cylindrospermopsis...
May 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28507706/ph-and-ligand-induced-release-of-loads-from-dna-acrylamide-hydrogel-microcapsules
#4
Wei-Ching Liao, Sivan Lilienthal, Jason S Kahn, Marianna Riutin, Yang Sung Sohn, Rachel Nechushtai, Itamar Willner
Herein, a method to construct stimuli-responsive DNA-acrylamide-based hydrogel microcapsules has been presented. This method involves the use of polyacrylamide chains modified with predesigned nucleic acid hairpin units and optionally single-strand tethers that provide the required hybridization and recognition functions to yield substrate-loaded stimuli-responsive hydrogel-based microcapsules. The synthesis of the microcapsules involves the loading of CaCO3 microparticles with the respective load substrates and the functionalization of the CaCO3 template particles with nucleic acid promoter units...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28507156/rna-primer-primase-complexes-serve-as-the-signal-for-polymerase-recycling-and-okazaki-fragment-initiation-in-t4-phage-dna-replication
#5
Michelle M Spiering, Philip Hanoian, Swathi Gannavaram, Stephen J Benkovic
The opposite strand polarity of duplex DNA necessitates that the leading strand is replicated continuously whereas the lagging strand is replicated in discrete segments known as Okazaki fragments. The lagging-strand polymerase sometimes recycles to begin the synthesis of a new Okazaki fragment before finishing the previous fragment, creating a gap between the Okazaki fragments. The mechanism and signal that initiate this behavior-that is, the signaling mechanism-have not been definitively identified. We examined the role of RNA primer-primase complexes left on the lagging ssDNA from primer synthesis in initiating early lagging-strand polymerase recycling...
May 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28496969/synthetic-approaches-to-nucleopeptides-containing-all-four-nucleobases-and-nucleic-acid-binding-studies-on-a-mixed-sequence-nucleo-oligolysine
#6
Giovanni N Roviello, Domenica Musumeci
In this article we describe two solid-phase synthetic routes to obtain a nucleo-oligolysine α-peptide containing all four natural nucleobases. The first one is based on the oligomerization of the nucleobase-containing monomers, easily synthesized as herein described. The second strategy has the advantage of avoiding the solution synthesis of the monomeric building blocks, leading to the final nucleopeptide by direct solid-phase couplings of the suitably protected nucleobases with the free amino groups on the growing peptide chain still anchored to the resin...
July 16, 2016: RSC Advances
https://www.readbyqxmd.com/read/28489550/a-fully-integrated-wireless-soc-for-motor-function-recovery-after-spinal-cord-injury
#7
Yi-Kai Lo, Yen-Cheng Kuan, Stanislav Culaclii, Brian Kim, Po-Min Wang, Chih-Wei Chang, Jonathan A Massachi, Minji Zhu, Kuanfu Chen, Parag Gad, V Reggie Edgerton, Wentai Liu
This paper presents a wirelessly powered, fully integrated system-on-a-chip (SoC) supporting 160-channel stimulation, 16-channel recording, and 48-channel bio-impedance characterization to enable partial motor function recovery through epidural spinal cord electrical stimulation. A wireless transceiver is designed to support quasi full-duplex data telemetry at a data rate of 2 Mb/s. Furthermore, a unique in situ bio-impedance characterization scheme based on time-domain analysis is implemented to derive the Randles cell electrode model of the electrode-electrolyte interface...
May 5, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28489083/exploring-biomolecular-energy-landscapes
#8
Jerelle A Joseph, Konstantin Röder, Debayan Chakraborty, Rosemary G Mantell, David J Wales
The potential energy landscape perspective provides both a conceptual and a computational framework for predicting, understanding and designing molecular properties. In this Feature Article, we highlight some recent advances that greatly facilitate structure prediction and analysis of global thermodynamics and kinetics in proteins and nucleic acids. The geometry optimisation procedures, on which these calculations are based, can be accelerated significantly using local rigidification of selected degrees of freedom, and through implementations on graphics processing units...
May 10, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28488811/resolution-of-occlusive-carotid-artery-thrombus-treated-with-anticoagulation-as-demonstrated-on-duplex-ultrasonography
#9
Laura Stein, John W Liang, Jesse Weinberger
BACKGROUND AND PURPOSE: While the majority of cerebral ischemic events due to carotid occlusive disease result from atherosclerotic plaque rupture, intraluminal carotid artery thrombus occasionally occurs in patients without preexisting carotid atherosclerosis. Identification of nonatherosclerotic thrombus as the cause of the carotid occlusive disease can obviate the need for an interventional procedure, and resolution of thrombus can be monitored with B-mode duplex ultrasonography. METHODS: We reviewed 3 patients treated on The Mount Sinai Hospital Stroke Unit with anticoagulation for nonatherosclerotic carotid thrombi and followed with serial Doppler ultrasonogrpahy for resolution of thrombus...
May 10, 2017: Journal of Neuroimaging: Official Journal of the American Society of Neuroimaging
https://www.readbyqxmd.com/read/28485278/30-day-results-from-prospective-multi-specialty-evaluation-of-carotid-artery-stenting-using-the-cguard-micronet-covered-embolic-prevention-stent-system-in-real-world-multicenter-clinical-practice-the-iron-guard-study
#10
Francesco Speziale, Laura Capoccia, Pasqualino Sirignano, Wassim Mansour, Chiara Pranteda, Renato Casana, Carlo Setacci
AIMS: The aim of the present study was to evaluate peri-procedural and 30-day outcomes in a prospective series of patients treated with the CGuard embolic prevention stent system (EPS). METHODS AND RESULTS: From April 2015 to June 2016 a physician-initiated prospective multicenter study was performed in 200 consecutive patients admitted to protected carotid artery stenting (CAS) and treated using CGuard EPS in twelve Vascular Centers. Outcome measures were: technical success, periprocedural (0-24 hours) and post- procedural (24hours-30 days) major and minor strokes, death, acute myocardial infarction (AMI), transient ischemic attack (TIA), and external carotid occlusion...
May 9, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28476680/an-inguinal-approach-to-complex-extravesical-ureteral-reimplantation
#11
Adam S Howe, Lane S Palmer
OBJECTIVE: To report our experience in applying the Lich-Gregoir extravesical ureteral reimplantation (EVR) approach to complex cases (megaureter, duplex systems) through a small inguinal incision, with the goal of minimizing invasiveness. MATERIALS & METHODS: We reviewed the records of all patients who underwent common sheath and/or tapered EVR through an inguinal incision. Patient characteristics and reflux grade were obtained, and outcomes were assessed. The technique involved a 2 cm incision made in the lowest inguinal skin crease, standard cord exposure and lateral retraction, opening the floor of the canal to isolate the ureter...
May 2, 2017: Urology
https://www.readbyqxmd.com/read/28475957/double-loop-hairpin-probe-and-doxorubicin-loaded-gold-nanoparticles-for-the-ultrasensitive-electrochemical-sensing-of-microrna
#12
Yiyi Tao, Dan Yin, Mingchao Jin, Jie Fang, Tao Dai, Yi Li, Yuxia Li, Qinli Pu, Guoming Xie
An electrochemical microRNA (miRNA) analysis platform by combining double-loop hairpin probe (DHP) and doxorubicin-loaded gold nanoparticles (AuNPs@Dox) for ultrasensitive miRNA detection is proposed. Firstly, we here report a DHP that is simultaneously engineered to incorporate a miRNA recognition sequence, an output segment and output's complementary fragment. The important aspect of this hairpin probe is that it would not be degraded by duplex specific nuclease (DSN) and circumvents elaborately chemical modification disadvantages encountered by classic molecular beacon...
May 2, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28470070/h-bond-self-assembly-folding-versus-duplex-formation
#13
Diego Núñez-Villanueva, Giulia Iadevaia, Alexander E Stross, Michael A Jinks, Jonathan A Swain, Christopher A Hunter
Linear oligomers equipped with complementary H-bond donor (D) and acceptor (A) sites can interact via intermolecular H-bonds to form duplexes or fold via intramolecular H-bonds. These competing equilibria have been quantified using NMR titration and dilution experiments for seven systems featuring different recognition sites and backbones. For all seven architectures, duplex formation is observed for homo-sequence 2-mers (AA·DD) where there are no competing folding equilibria. The corresponding hetero-sequence AD 2-mers also form duplexes, but the observed self-association constants are strongly affected by folding equilibria in the monomeric states...
May 4, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28464482/dna-films-containing-the-artificial-nucleobase-imidazole-mediate-charge-transfer-in-a-silver-i-responsive-way
#14
J Christian Léon, Zhe She, Ajar Kamal, Mohtashim Hassan Shamsi, Jens Müller, Heinz-Bernhard Kraatz
The first sequence-dependent study of DNA films containing metal-mediated base pairs was performed to investigate the charge transfer resistance (RCT ) of metal-modified DNA. The imidazole (Im) deoxyribonucleoside was chosen as a highly Ag(I) -specific ligandoside for the formation of Im-Ag(I) -Im complexes within the duplexes. This new class of site-specifically metal-modified DNA films was characterized by UV, circular dichroism (CD), and X-ray photoelectron spectroscopy (XPS). The electrochemical properties of these systems were investigated by means of electron impedance spectroscopy and scanning electrochemical microscopy...
May 2, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28463782/asymptomatic-polyvascular-disease-and-the-risks-of-cardiovascular-events-and-all-cause-death
#15
Qian Zhang, Anxin Wang, Shufeng Zhang, Na Li, Shengyun Chen, Yaqing Zhang, Yong Zhou, Shouling Wu, Xingquan Zhao
BACKGROUND AND AIMS: Atherosclerosis is a diffuse and systemic disease. We aimed to assess prevalence and outcome of extracoronary polyvascular disease (polyVD) in the asymptomatic Chinese community population. METHODS: A random sample of 5440 participants aged 40 years or older were enrolled in the Asymptomatic Polyvascular Abnormalities Community Study from 2010 to 2011. Intracranial artery stenosis, extracranial artery stenosis, and lower extremity artery disease were detected by transcranial Doppler and duplex sonography, and by calculating the ankle brachial index...
April 21, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28455973/markers-of-arterial-health-could-serve-as-accurate-non-invasive-predictors-of-human-biological-and-chronological-age
#16
Alexander Fedintsev, Daria Kashtanova, Olga Tkacheva, Irina Strazhesko, Anna Kudryavtseva, Ancha Baranova, Alexey Moskalev
The decline in functional capacity is unavoidable consequence of the process of aging. While many anti-aging interventions have been proposed, clinical investigations into anti-aging medicine are limited by lack of reliable techniques for evaluating the rate of ageing. Here we present simple, accurate and cost-efficient techniques for estimation of human biological age, Male and Female Arterial Indices. We started with developing a model which accurately predicts chronological age. Using machine learning, we arrived on a set of four predictors, all of which reflect the functioning of the cardiovascular system...
April 2017: Aging
https://www.readbyqxmd.com/read/28455146/detection-of-anomalous-cervical-internal-carotid-artery-branches-by-colour-duplex-ultrasound
#17
K Busch, R Chandra, T Buckenham, H Kiat
OBJECTIVES: Conventional anatomical descriptions of the cervical internal carotid artery (ICA) report that no branches arise from this segment. However reports of ICA branches exist. The study aim was to determine the prevalence of anomalous branches of the ICA using colour duplex ultrasound (CDU). METHODS: Four hundred consecutive patients (800 carotid vessels) referred to a tertiary hospital vascular laboratory for investigation of carotid disease were included...
April 25, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28451167/homochiral-oligomers-with-highly-flexible-backbones-form-stable-h-bonded-duplexes
#18
Diego Núñez-Villanueva, Christopher A Hunter
Two homochiral building blocks featuring a protected thiol, an alkene and a H-bond recognition unit (phenol or phosphine oxide) have been prepared. Iterative photochemical thiol-ene coupling reactions were used to synthesize oligomers containing 1-4 phosphine oxide and 1-4 phenol recognition sites. Length-complementary H-bond donor and H-bond acceptor oligomers were found to form stable duplexes in toluene. NMR titrations and thermal denaturation experiments show that the association constant and the enthalpy of duplex formation increase significantly for every additional H-bonding unit added to the chain...
January 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28447587/appropriateness-of-duplex-ultrasound-assessment-on-venous-system-of-the-legs-a-two-month-preliminary-analysis
#19
Silvia Stegher, Maria Teresa Occhiuto, Daniela P Mazzaccaro, Paolo Carlo Righini, Alfredo Modafferi, Giovanni Malacrida, Giovanni Nano
AIM: The goal of this study is to evaluate the appropriateness to prescribe venous ultrasound of lower limbs. MATERIAL OF STUDY: Over a two months period, 1005 Duplex scans were performed by our Vascular Surgery Unit; out of the total, 225 exams were conducted on venous system of lower limbs. We retrospectively analyzed appropriateness of prescription (according to Lombardy District indications), urgency of prescription, time-lapse between application for the exams and its execution, positive or negative results, National Health System's sustained cost...
2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/28445747/physical-and-functional-characterization-of-a-viral-genome-maturation-complex
#20
Teng-Chieh Yang, David Ortiz, Qin Yang, Rolando W De Angelis, Saurarshi J Sanyal, Carlos E Catalano
Genome packaging is strongly conserved in the complex double-stranded DNA viruses, including the herpesviruses and many bacteriophages. In these cases, viral DNA is packaged into a procapsid shell by a terminase enzyme. The packaging substrate is typically a concatemer composed of multiple genomes linked in a head-to-tail fashion, and terminase enzymes perform two essential functions: 1) excision of a unit length genome from the concatemer (genome maturation) and 2) translocation of the duplex into a procapsid (genome packaging)...
April 25, 2017: Biophysical Journal
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