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Duplex systems

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https://www.readbyqxmd.com/read/28805877/a-dna-bipedal-nanowalker-with-a-piston-like-expulsion-stroke
#1
Q Y Yeo, I Y Loh, S R Tee, Y H Chiang, J Cheng, M H Liu, Z S Wang
Artificial molecular walkers beyond burn-bridge designs are important for nanotechnology, but their systematic development remains difficult. Herein, we have reported a new rationally designed DNA walker-track system and experimentally verified a previously proposed general expulsion regime for implementing non-burn-bridge nanowalkers. The DNA walker has an optically powered engine motif that reversibly extends and contracts the walker via a quadruplex-duplex conformational change. The walker's extension is an energy-absorbing and force-generating process, which drives the walker's leg dissociation off-track in a piston-like expulsion stroke...
August 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/28798779/one-swallow-does-not-a-summer-make-but-many-swallows-do-accumulating-clinical-evidence-for-nearly-eliminated-peri-procedural-and-30-day-complications-with-mesh-covered-stents-transforms-the-carotid-revascularisation-field
#2
REVIEW
Piotr Musiałek, L Nelson Hopkins, Adnan H Siddiqui
Atherosclerotic carotid artery stenosis (CS) continues to be a common cause of acute ischaemic stroke. Optimised medical therapy (OMT), the first-line treatment modality in CS, may reduce or delay - but it does not abolish - CS-related strokes. As per current AHA/ASA and ESC/ESVS/ESO guidelines, carotid artery stenting (CAS) is a less-invasive alternative to carotid endarterectomy (CEA) for CS revascularisation in primary and secondary stroke prevention. Ten-year follow-up from the CREST trial in patients with symptomatic and asymptomatic CS confirmed equipoise of CAS and CEA in the primary endpoint...
2017: Postępy W Kardiologii Interwencyjnej, Advances in Interventional Cardiology
https://www.readbyqxmd.com/read/28798248/giant-hydronephrosis-in-a-case-of-ureterocele-with-duplex-system-an-entity-yet-not-reported
#3
Ruchir Aeron, Ashok Kumar Sokhal, Manoj Kumar, Satyanarayan Sankhwar
Ureterocele, which is a cystic dilatation of the terminal ureter, is usually associated with the upper moiety in a case of the duplex system. Giant hydronephrosis, a rare entity, is usually due to pelviureteric junction obstruction and is usually diagnosed in infants and children. We report a unique case of a unilateral complete duplex system with ureterocele with giant hydronephrosis of the upper moiety in an adult woman presenting as an abdominal lump. To the best of our knowledge, this is the first case of giant hydronephrosis associated with ureterocele in an adult patient...
August 10, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28795494/an-extended-high-frequency-ultrasound-protocol-for-detection-of-vessel-wall-inflammation
#4
H Zachrisson, C Svensson, A Dremetsika, P Eriksson
OBJECTIVE: The aim of this study was to evaluate an extended protocol of the large vessels using high-frequency duplex ultrasound (DUS) for detection of vessel wall inflammation. METHODS: Fifty-eight patients performed a DUS examination where arteritis could not be excluded. All DUS examinations were performed using ACUSON S2000 TM ultrasound system (Siemens Medical Solutions USA, Inc.). High-frequency linear transducers were used (18L6 MHz, 9L4MHz) or curve linear for the aortic arch (6C2 MHz)...
August 9, 2017: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/28783358/long-range-ordering-of-blunt-ended-dna-tiles-on-supported-lipid-bilayers
#5
Nicole Avakyan, Justin Conway, Hanadi F Sleiman
Long-range ordering of DNA crossover tiles with blunt ends on lipid bilayers is investigated using atomic force microscopy. "Blunt-ended" tiles do not have single-stranded complementary ends, and thus instead of assembling via base-pairing, they can interact by π-stacking of their duplex ends. This work demonstrates that the balance of base π-stacking interactions between the ends of DNA duplexes, cholesterol-mediated DNA anchoring and electrostatic DNA binding to supported lipid bilayers (SLBs) presents an opportunity to build dynamic materials with long-range order on a soft support...
August 7, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28781236/structural-variation-of-type-i-f-crispr-rna-guided-dna-surveillance
#6
Patrick Pausch, Hanna Müller-Esparza, Daniel Gleditzsch, Florian Altegoer, Lennart Randau, Gert Bange
CRISPR-Cas systems are prokaryotic immune systems against invading nucleic acids. Type I CRISPR-Cas systems employ highly diverse, multi-subunit surveillance Cascade complexes that facilitate duplex formation between crRNA and complementary target DNA for R-loop formation, retention, and DNA degradation by the subsequently recruited nuclease Cas3. Typically, the large subunit recognizes bona fide targets through the PAM (protospacer adjacent motif), and the small subunit guides the non-target DNA strand. Here, we present the Apo- and target-DNA-bound structures of the I-Fv (type I-F variant) Cascade lacking the small and large subunits...
August 2, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28780124/antitumour-activity-of-resveratrol-on-human-melanoma-cells-a-possible-mechanism-related-to-its-interaction-with-malignant-cell-telomerase
#7
Chiara Platella, Serena Guida, Laura Bonmassar, Angelo Aquino, Enzo Bonmassar, Giampiero Ravagnan, Daniela Montesarchio, Giovanni N Roviello, Domenica Musumeci, Maria Pia Fuggetta
BACKGROUND: trans-resveratrol (tRES) is a polyphenolic stilbene found in plant products which has attracted great attention because of its antioxidant, anti-inflammatory and anticancer properties. METHODS: The possible correlation between tRES-induced suppression of melanoma cell growth and its influence on telomerase expression has been investigated by biological assays. Moreover, in order to gain new knowledge about possible mechanisms of action of tRES as antineoplastic agent, its interaction with biologically relevant secondary structure-forming DNA sequences, its aggregation properties and copper-binding activity have been studied by CD, UV and fluorescence spectroscopies...
August 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28777560/a-critical-crosscatalytic-relationship-determines-the-outcome-of-competition-in-a-replicator-network
#8
Tamara Kosikova, Douglas Philp
A network of two synthetic replicators exhibits a critical unidirectional crosscatalytic relationship that directs competing replication processes. In this network, nitrone N bearing a 6-methylamidopyridine recognition site can participate in two 1,3-dipolar cycloaddition reactions with two maleimides that differ in the relative position of their carboxylic acid recognition site - either para (Mp) or meta (Mm) relative to the maleimide ring. These two cycloaddition reactions create replicators trans-Tp and trans-Tm...
August 4, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28773996/surface-texturing-plasma-nitriding-duplex-treatment-for-improving-tribological-performance-of-aisi-316-stainless-steel
#9
Naiming Lin, Qiang Liu, Jiaojuan Zou, Junwen Guo, Dali Li, Shuo Yuan, Yong Ma, Zhenxia Wang, Zhihua Wang, Bin Tang
Surface texturing-plasma nitriding duplex treatment was conducted on AISI 316 stainless steel to improve its tribological performance. Tribological behaviors of ground 316 substrates, plasma-nitrided 316 (PN-316), surface-textured 316 (ST-316), and duplex-treated 316 (DT-316) in air and under grease lubrication were investigated using a pin-on-disc rotary tribometer against counterparts of high carbon chromium bearing steel GCr15 and silicon nitride Si₃N₄ balls. The variations in friction coefficient, mass loss, and worn trace morphology of the tested samples were systemically investigated and analyzed...
October 27, 2016: Materials
https://www.readbyqxmd.com/read/28771608/detection-and-quantification-of-beef-and-pork-materials-in-meat-products-by-duplex-droplet-digital-pcr
#10
Yicun Cai, Yuping He, Rong Lv, Hongchao Chen, Qiang Wang, Liangwen Pan
Meat products often consist of meat from multiple animal species, and inaccurate food product adulteration and mislabeling can negatively affect consumers. Therefore, a cost-effective and reliable method for identification and quantification of animal species in meat products is required. In this study, we developed a duplex droplet digital PCR (dddPCR) detection and quantification system to simultaneously identify and quantify the source of meat in samples containing a mixture of beef (Bos taurus) and pork (Sus scrofa) in a single digital PCR reaction tube...
2017: PloS One
https://www.readbyqxmd.com/read/28770201/follicular-conjunctivitis-due-to-chlamydia-felis-case-report-review-of-the-literature-and-improved-molecular-diagnostics
#11
Juliana Wons, Ralph Meiller, Antonio Bergua, Christian Bogdan, Walter Geißdörfer
A 29-year-old woman presented with unilateral, chronic follicular conjunctivitis since 6 weeks. While the conjunctival swab taken from the patient's eye was negative in a Chlamydia (C.) trachomatis-specific PCR, C. felis was identified as etiological agent using a pan-Chlamydia TaqMan-PCR followed by sequence analysis. A pet kitten of the patient was found to be the source of infection, as its conjunctival and pharyngeal swabs were also positive for C. felis. The patient was successfully treated with systemic doxycycline...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/28757251/the-molecular-architecture-for-rna-guided-rna-cleavage-by-cas13a
#12
Liang Liu, Xueyan Li, Jun Ma, Zongqiang Li, Lilan You, Jiuyu Wang, Min Wang, Xinzheng Zhang, Yanli Wang
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM structure of the LbuCas13a-crRNA complex. The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and Cas13a protein and crRNA undergo a significant conformational change upon target RNA binding...
July 21, 2017: Cell
https://www.readbyqxmd.com/read/28754940/a-device-that-operates-within-a-self-assembled-3d-dna-crystal
#13
Yudong Hao, Martin Kristiansen, Ruojie Sha, Jens J Birktoft, Carina Hernandez, Chengde Mao, Nadrian C Seeman
Structural DNA nanotechnology finds applications in numerous areas, but the construction of objects, 2D and 3D crystalline lattices and devices is prominent among them. Each of these components has been developed individually, and most of them have been combined in pairs. However, to date there are no reports of independent devices contained within 3D crystals. Here we report a three-state 3D device whereby we change the colour of the crystals by diffusing strands that contain dyes in or out of the crystals through the mother-liquor component of the system...
August 2017: Nature Chemistry
https://www.readbyqxmd.com/read/28750042/subclinical-thyroid-dysfunction-and-risk-of-carotid-atherosclerosis
#14
Hosu Kim, Tae Hyuk Kim, Hye In Kim, So Young Park, Young Nam Kim, Seonwoo Kim, Min-Ji Kim, Sang-Man Jin, Kyu Yeon Hur, Jae Hyeon Kim, Moon-Kyu Lee, Yong-Ki Min, Jae Hoon Chung, Mira Kang, Sun Wook Kim
BACKGROUND: The effect of subclinical thyroid dysfunction on vascular atherosclerosis remains uncertain. The objective of this study was to elucidate the association between sustained subclinical thyroid dysfunction and carotid plaques, which are an early surrogate marker of systemic atherosclerosis. METHODS: The study included 21,342 adults with consistent thyroid hormonal status on serial thyroid function tests (TFTs) and carotid artery duplex ultrasonography at a health screening center between 2007 and 2014...
2017: PloS One
https://www.readbyqxmd.com/read/28745059/near-infrared-neodymium-tag-for-quantifying-targeted-biomarker-and-counting-its-host-circulating-tumor-cells
#15
Chunlan Liu, Shu Lu, Limin Yang, Peijie Chen, Peiming Bai, Qiuquan Wang
Quantitative information on a targeted analyte in a complex biological system is the most basic premise for understanding its involved mechanisms, and thus precise diagnosis of a disease if it is a so-called biomarker. Here, we designed and synthesized a neodymium (Nd)-cored tag [1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid (DOTA)-Nd complex together with a light-harvesting antenna aminofluorescein (AMF, λex/em = 494/520 nm), AMF-DOTA-Nd] with duplex signals, second near-infrared (NIR) window luminescence (λem = 1065 nm, 2...
August 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28740380/inhibition-of-cell-proliferation-through-an-atp-responsive-co-delivery-system-of-doxorubicin-and-bcl-2-sirna
#16
Jianxu Zhang, Yudi Wang, Jiawen Chen, Xiao Liang, Haobo Han, Yan Yang, Quanshun Li, Yanbo Wang
Herein, DNA duplex was constructed through the hybridization of adenosine triphosphate (ATP)-responsive aptamer and its cDNA in which GC-rich motif could be used to load doxorubicin (DOX), and then, cationic polymer PEI25K was used as a carrier to simultaneously condense DOX-Duplex and Bcl-2 siRNA to prepare the ternary nanocomplex polyethylenimine (PEI)/DOX-Duplex/siRNA. The ATP concentration gradient between the cytosol and extracellular environment could achieve the stable loading of DOX in duplex and the rapid drug release in an ATP-responsive manner...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28737379/an-enzyme-free-dna-circuit-assisted-graphene-oxide-enhanced-fluorescence-anisotropy-assay-for-microrna-detection-with-improved-sensitivity-and-selectivity
#17
Shu Jun Zhen, Xue Xiao, Chun Hong Li, Cheng Zhi Huang
Graphene oxide (GO) has been proven as an outstanding fluorescence anisotropy (FA) amplifier. But the traditional GO amplified FA assays lack high sensitivity because of the 1:1 binding ratio between target and dye-modified probe. Herein, we report a new target-catalyzed hairpin assembly (CHA), an enzyme-free DNA circuit, assisted GO amplified FA strategy for microRNA-21 (miRNA-21) detection. In the presence of miRNA-21, the CHA was initiated and plenty of H1-H2 duplexes were produced continuously. The obtained H1-H2 duplex could induce the formation of a H1-H2-probe DNA (pDNA) complex by the toehold-mediated strand exchange reaction, which led the dye-modified pDNA to leave away from the GO surface, resulting in a decreased FA of the system...
July 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28734608/sequence-specific-dna-solid-phase-extraction-in-an-on-chip-monolith-towards-detection-of-antibiotic-resistance-genes
#18
Radim Knob, Daniel B Nelson, Richard A Robison, Adam T Woolley
Antibiotic resistance of bacteria is a growing problem and presents a challenge for prompt treatment in patients with sepsis. Currently used methods rely on culturing or amplification; however, these steps are either time consuming or suffer from interference issues. A microfluidic device was made from black polypropylene, with a monolithic column modified with a capture oligonucleotide for sequence selective solid-phase extraction of a complementary target from a lysate sample. Porous properties of the monolith allow flow and hybridization of a target complementary to the probe immobilized on the column surface...
July 10, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28731353/altering-dna-programmable-colloidal-crystallization-paths-by-modulating-particle-repulsion
#19
Mary X Wang, Jeffrey D Brodin, Jaime A Millan, Soyoung E Seo, Martin Girard, Monica Olvera de la Cruz, Byeongdu Lee, Chad A Mirkin
Colloidal crystal engineering with DNA can be used to realize precise control over nanoparticle (NP) arrangement. Here, we investigate a case of DNA-based assembly where the properties of DNA as a polyelectrolyte brush are employed to alter a hybridization-driven NP crystallization pathway. Using the coassembly of DNA-conjugated proteins and spherical gold nanoparticles (AuNPs) as a model system, we explore how steric repulsion between noncomplementary, neighboring NPs due to overlapping DNA shells can influence their ligand-directed behavior...
July 21, 2017: Nano Letters
https://www.readbyqxmd.com/read/28730431/protocols-for-molecular-dynamics-simulations-of-rna-nanostructures
#20
Taejin Kim, Wojciech K Kasprzak, Bruce A Shapiro
Molecular dynamics (MD) simulations have been used as one of the main research tools to study a wide range of biological systems and bridge the gap between X-ray crystallography or NMR structures and biological mechanism. In the field of RNA nanostructures, MD simulations have been used to fix steric clashes in computationally designed RNA nanostructures, characterize the dynamics, and investigate the interaction between RNA and other biomolecules such as delivery agents and membranes.In this chapter we present examples of computational protocols for molecular dynamics simulations in explicit and implicit solvent using the Amber Molecular Dynamics Package...
2017: Methods in Molecular Biology
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