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Human papillomavirus testing

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https://www.readbyqxmd.com/read/28205267/comparison-of-advansure-hpv-genoblot-and-hybrid%C3%A2-capture%C3%A2-2-assays-for-detection-of-hpv-infection
#1
Kiwoong Ko, Shinae Yu, Min-Jung Kwon, Eun Hee Lee, Hee-Yeon Woo, Hyosoon Park
BACKGROUND: We sought to compare the performance of the AdvanSure assay to the Hybrid Capture (HC) 2 for the detection of high-risk human papillomavirus (HR HPV). METHODS: A total of 855 cervical swab specimens were obtained. We submitted all specimens for HR HPV detection with HC2 and the AdvanSure assay. We subsequently analyzed discordant results and specimens that were positive on both assays using restriction fragment mass polymorphism (RFMP) genotyping analysis...
February 15, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28203108/a-review-of-screening-strategies-for-cervical-cancer-in-human-immunodeficiency-virus-positive-women-in-sub-saharan-africa
#2
REVIEW
Manuela Viviano, Pierre DeBeaudrap, Pierre-Marie Tebeu, Jovanny T Fouogue, Pierre Vassilakos, Patrick Petignat
Cervical cancer (CC) is a leading cause of cancer-related death and a major public health issue in sub-Saharan Africa. This heavy burden parallels that of the human immunodeficiency virus (HIV) infection, which increases the risk of developing CC. Despite the progressive reduction of HIV prevalence in the past decade, the CC incidence and mortality rates in sub-Saharan Africa remain high. The heterogeneity of the distribution of the two diseases in the African continent, together with the different availability of human and material resources, stands in the way of finding an appropriate screening strategy...
2017: International Journal of Women's Health
https://www.readbyqxmd.com/read/28195317/human-papillomavirus-self-sampling-for-screening-non-attenders-opt-in-pilot-implementation-with-electronic-communication-platforms
#3
Janni Uyen Hoa Lam, Matejka Rebolj, Ditte Møller Ejegod, Helle Pedersen, Carsten Rygaard, Elsebeth Lynge, Louise Thirstrup Thomsen, Susanne Krüger Kjaer, Jesper Bonde
In organized cervical screening programs, typically 25% of the invited women do not attend. The Copenhagen Self-sampling Initiative (CSi) aimed to gain experiences on participation among screening non-attenders in the Capital Region of Denmark. Here, we report on the effectiveness of different communication platforms used in the pilot with suggestions for strategies prior to a full-implementation. Moreover, an innovative approach using self-sampling brushes with unique radio frequency identification chips allowed for unprecedented levels patient identification safety...
February 13, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28186384/women-s-perspectives-on-human-papillomavirus-self-sampling-in-the-context-of-the-uk-cervical-screening-programme
#4
Denitza Williams, Myfanwy Davies, Alison Fiander, Daniel Farewell, Sharon Hillier, Kate Brain
BACKGROUND: Testing for human papillomavirus (HPV) is being incorporated into the cervical screening programme, with the probable future introduction of HPV as a primary test and a possibility of HPV self-sampling. In anticipation of this development, we sought to inform future policy and practice by identifying potential barriers to HPV self-sampling. METHODS: A cross-sectional survey of 194 women aged 20-64 years was conducted. Logistic regression analysis was used to identify determinants of self-sampling intentions...
February 10, 2017: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/28182076/vaginal-infections-of-albanian-women-infected-with-hpv-and-their-impact-in-intraepithelial-cervical-lesions-evidenced-by-pap-test
#5
Elsa S Kone, Avenir D Balili, Piro D Paparisto, Xheladin R Ceka, Elizana D Petrela
BACKGROUND: Cervical cytology is the best single method for large screening of the population in identifying precancerous lesions of the uterine cervix. AIM: To estimate the frequency of human papillomavirus (HPV) positivity in a group of Albanian women, the prevalence of vaginal coinfections, and the relationship of coinfections with HPV, as well as their role in metaplasia or cervical intraepithelial lesions (CIN). MATERIALS AND METHODS: In this retrospective study, 2075 vaginal smears were examined...
January 2017: Journal of Cytology
https://www.readbyqxmd.com/read/28181989/-influence-of-age-in-the-prevalence-of-high-risk-human-papiloma-virus-in-women-with-pre-neoplasic-cervical-lesions-in-navarra-spain
#6
María Rosario Mercado Gutiérrez, Carolina Arean Cuns, María Luisa Gómez Dorronsoro, Irene Paniello Alastruey, Fermin Mallor Giménez, Maria Dolores Lozano Escario, Mercedes Santamaría Martínez
OBJECTIVE: Cervical carcinoma (CC) is the second cause of death among women aged 15 and 44 in Spain. CC is linked to hig-risk human papillomavirus (HR-HPV) infection and its prevalence varies according age and geographical region. The awereness of the latter is essential for public health prevention efforts. The aim was to study the age related in HR-HPV genotypes in cytologies with squamous intraepithelial lesion (SIL). METHODS: From a total of 67,935 ginecologic cytologies over a four-year period, we selected cytologic specimens with SIL...
9, 2017: Revista Española de Salud Pública
https://www.readbyqxmd.com/read/28179702/knowledge-and-practices-of-nurses-on-cervical-cancer-hpv-and-hpv-vaccine-in-cankiri-state-hospital-turkey
#7
Ilknur Gol, OzUm Erkin
OBJECTIVE: To determine knowledge and practices of nurses on cervical cancer, human papillomavirus and its vaccine. METHODS: This descriptive study was conducted at Cankiri Karatekin University, Cankiri, Turkey, from December 2015 to January 2016, and included nurses. . A survey was used to collect data about socio-demographic features and knowledge and practices on cervical cancer and screening, signs and symptoms of cervical cancer and human papillomavirus vaccine...
December 2016: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28178116/genital-human-papillomavirus-infection-in-indian-hiv-seropositive-men-who-have-sex-with-men
#8
Anantharam Raghavendran, Alexandra L Hernandez, Shelly Lensing, Manu Gnanamony, Rajiv Karthik, Murgesan Sivasubramanian, Rajesh Kannangai, Priya Abraham, Dilip Mathai, Joel M Palefsky
BACKGROUND: The incidence of penile cancer in Indian men is high. Little is known about genital human papillomavirus (HPV) infection in Indian HIV-seropositive men who have sex with men (MSM), a population that may be at particularly high risk for genital HPV infection and, potentially, penile cancer. In this study, we assessed the prevalence and risk factors for genital HPV infection in this population. DESIGN AND METHODS: Three hundred HIV-seropositive MSM were recruited from 2 clinical sites in India...
March 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28171876/clinical-value-of-the-epstein-barr-virus-and-p16-status-in-patients-with-nasopharyngeal-carcinoma-a-single-centre-study-in-japan
#9
Yuki Saito, Tetsuo Ushiku, Go Omura, Kazuo Yasuhara, Masafumi Yoshida, Wataru Takahashi, Mizuo Ando, Masashi Fukayama, Tatsuya Yamasoba
BACKGROUND: The clinical significance of the Epstein-Barr virus (EBV) status and p16 expression was unknown in nasopharyngeal carcinoma (NPC). METHODS: We retrospectively studied our pathology database for 13 years to determine the prevalence of EBV and p16 expression and their association with prognosis in cases of NPC. We performed immunohistochemistry for the p16 protein and in situ hybridization (ISH) for EBV-encoded small RNAs and human papillomavirus (HPV) DNA...
February 8, 2017: ORL; Journal for Oto-rhino-laryngology and its related Specialties
https://www.readbyqxmd.com/read/28166180/therapeutic-vaccine-against-primate-papillomavirus-infections-of-the-cervix
#10
Emeline Ragonnaud, Anne-Marie C Andersson, Silmi Mariya, Anders G Pedersen, Robert D Burk, Antonella Folgori, Stefano Colloca, Riccardo Cortese, Alfredo Nicosia, Joko Pamungkas, Diah Iskandriati, Peter J Holst
Currently available prophylactic vaccines have no therapeutic efficacy for preexisting human papillomavirus (HPVs) infections, do not target all oncogenic HPVs and are insufficient to eliminate the burden of HPV induced cancer. We aim to develop an alternative HPV vaccine which is broadly effective and capable of clearing preexisting infection. In an initial attempt to develop a broadly reactive therapeutic vaccine, we designed a putative papillomavirus (PV) ancestor antigen (circulating sequence derived antigenic sequences E1E2-CDSE1E2) based on the conserved E1 and E2 protein sequences from existing oncogenic HPV strains...
February 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/28164665/human-papillomavirus-genotyping-by-surface-plasmon-resonance-based-test
#11
Xiaoli He, Pei Yang, He Wang, Yue Wang, Shanling Liu
BACKGROUND: This study was performed to evaluate the surface plasmon resonance-based (SPR) test with the W2600 System of GP Medical Technologies as a screening method for human papillomavirus (HPV) genotyping in China. METHODS: Between November 2012 and December 2013, the SPR test, hybrid capture II (HC2) test, and direct DNA sequencing assay were performed on 424 patients aged 22 - 65 years who attended the outpatient clinic at the Department of Obstetrics and Gynecology of West China Second University Hospital with diagnoses of chronic cervicitis or abnormal vaginal bleeding...
November 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28164495/isothermal-method-of-a-recombinase-polymerase-amplification-assay-for-the-detection-of-most-common-high-risk-human-papillomavirus-type-16-and-type-18-dna
#12
Biao Ma, Jiehong Fang, Ye Wang, Haizhen He, Mingyan Dai, Wei Lin, Wei Su, Mingzhou Zhang
BACKGROUND: Cervical cancer is a common gynecologic malignant tumor and has a great impact on women's health. Human papillomavirus (HPV) is implicated in cervical cancer and precancerous lesions and the two are possibly two stages of disease progression. With the technological development of molecular biology and epidemiology, detection and treatment of HPV has become an important means to prevent cervical cancer. METHODS: Here we present a novel, rapid, sensitive and specific isothermal method of recombinase polymerase amplification (RPA), which is established to detect the two most common high-risk human papillomavirus type 16 and type 18 DNA...
January 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28161547/the-use-of-chitosan-as-cationic-coating-or-gel-vehicle-for-polymeric-nanocapsules-increasing-penetration-and-adhesion-of-imiquimod-in-vaginal-tissue
#13
Luiza A Frank, Paula S Chaves, Camilo M D'Amore, Renata V Contri, Alejandro G Frank, Ruy C R Beck, Adriana R Pohlmann, Andreia Buffon, Silvia S Guterres
The human papillomavirus (HPV) infection, which is strongly related to cervical cancer, can be reduced by the topical application of imiquimod. Some strategies have been used to increase the adhesion and penetration of drugs through the vaginal mucosa. Two of them are the development of mucoadhesive semisolid formulations and the development of polymeric nanocarriers. In this paper, we hypothesize that the combined use of these two strategies results in a better performance of the formulation to retain imiquimod into the vaginal tissue...
February 1, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28161543/anal-study-in-immunocompetent-women-with-human-papillomavirus-related-lower-genital-tract-pathology
#14
Concepción Donaire, Marcos Reillo, Juan C Martínez-Escoriza, José A López-Fernández
OBJETIVE: To estimate the prevalence of anal dysplasia in immunocompetent women with cervical intraepithelial dysplasia. STUDY DESIGN: We did a prospective cohort study, in which we enrolled 166 women with gynecological pathology related to human papilloma virus (HPV) infection. All patients underwent an anal cytology and HPV detection. Statistical analysis with a 95% confidence interval was used for prevalence calculations. A Χ2 test and Fisher's exact one were used to determine differences between groups of qualitative variables...
January 19, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28161277/comparison-of-the-abbott-realtime-high-risk-hpv-test-and-the-roche-cobas-4800-hpv-test-using-urine-samples
#15
Myong Cheol Lim, Do-Hoon Lee, Sang-Hyun Hwang, Na Rae Hwang, Bomyee Lee, Hye Young Shin, Jae Kwan Jun, Chong Woo Yoo, Dong Ock Lee, Sang-Soo Seo, Sang-Yoon Park, Jungnam Joo
BACKGROUND: Human papillomavirus (HPV) testing based on cervical samples is important for use in cervical cancer screening. However, cervical sampling is invasive. Therefore, non-invasive methods for detecting HPV, such as urine samples, are needed. OBJECTIVES: For HPV detection in urine samples, two real-time PCR (RQ-PCR) tests, Roche cobas 4800 test (Roche_HPV; Roche Molecular Diagnostics) and Abbott RealTime High Risk HPV test (Abbott_HPV; Abbott Laboratories) were compared to standard cervical samples...
February 1, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28149707/virucidal-efficacy-of-a-sonicated-hydrogen-peroxide-system-trophon-%C3%A2-epr-following-european-and-german-test-methods
#16
Britta Becker, Birte Bischoff, Florian H H Brill, Eike Steinmann, Jochen Steinmann
Aim: The virucidal efficacy of an automated ultrasound probe disinfector (trophon(®) EPR) was evaluated in a three step procedure according to European and German test methods. This system uses sonicated hydrogen peroxide mist (35%) at elevated temperature (50°C) in a closed chamber with control of all parameters within a 7 minute cycle. Methods: In the first step of examination, the peroxide solution was tested in a quantitative suspension assay according to the Guideline of Deutsche Vereinigung zur Bekämpfung der Viruskrankheiten (DVV) e...
2017: GMS Hygiene and Infection Control
https://www.readbyqxmd.com/read/28144096/burden-of-cervical-cancer-and-role-of-screening-in-india
#17
Saurabh Bobdey, Jignasa Sathwara, Aanchal Jain, Ganesh Balasubramaniam
BACKGROUND: Cervical cancer is a major cause of cancer mortality in women and more than a quarter of its global burden is contributed by developing countries. In India, in spite of alarmingly high figures, there is no nationwide government-sponsored screening program. This study was conducted to assess the burden of cervical cancer in India and review the performance characteristics of available cervical cancer screening tools, so as to provide evidence-based recommendations for application of most practically suited screening test to be used in resource-poor field settings...
October 2016: Indian Journal of Medical and Paediatric Oncology
https://www.readbyqxmd.com/read/28141783/prevalence-of-anal-hpv-and-anal-dysplasia-in-hiv-infected-women-from-johannesburg-south-africa
#18
Bridgette J Goeieman, Cynthia S Firnhaber, Eefje Jong, Pam Michelow, Avril Swarts, Anna-Lise Williamson, Bruce Allan, Jennifer S Smith, Patricia Kegorilwe, Timothy J Wilkin
BACKGROUND: Anal cancer is a relatively common cancer among HIV-infected populations. There are limited data on the prevalence of anal high-risk human papillomavirus (HR-HPV) infection and anal dysplasia in HIV-infected women from resource-constrained settings. METHODS: A cross-sectional study of HIV-infected women age 25-65 recruited from an HIV clinic in Johannesburg, South Africa. Cervical and anal swabs were taken for conventional cytology and HR-HPV testing...
January 30, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/28141601/is-p16-an-adequate-surrogate-for-human-papillomavirus-status-determination
#19
Lauri Jouhi, Jaana Hagström, Timo Atula, Antti Mäkitie
PURPOSE OF REVIEW: In head and neck cancer management, immunoexpression of protein p16 is widely used as a surrogate marker for human papillomavirus (HPV) positivity due to challenges of direct HPV testing. Previously p16 has been reported to have good sensitivity but only moderate specificity in HPV-status determination. The aim of this review is to evaluate the existing information on sensitivity and specificity of p16 immunohistochemistry (IHC) in HPV-status determination among the recent studies...
January 30, 2017: Current Opinion in Otolaryngology & Head and Neck Surgery
https://www.readbyqxmd.com/read/28134480/fine-needle-aspiration-biopsy-of-hpv-related-squamous-cell-carcinoma-of-the-head-and-neck-current-ancillary-testing-methods-for-determining-hpv-status
#20
REVIEW
Cory T Bernadt, Brian T Collins
Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is a unique form of carcinoma that largely arises from the tonsillar tissue in the oropharynx. These tumors often present with cervical lymphadenopathy resulting in a fine needle aspiration (FNA) biopsy. Use of the cytology specimen to determine the HPV-status has significant prognostic and treatment implications as HPV-related tumors have a more favorable prognosis and response to nonsurgical therapies. While several different ancillary testing methods are available that have proven effective for determining HPV status in FNA specimens from HNSCCs, there is currently no consensus regarding HPV testing in this setting...
January 30, 2017: Diagnostic Cytopathology
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