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Human papillomavirus testing

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https://www.readbyqxmd.com/read/28632191/sexually-transmitted-infections-a-novel-screening-strategy-for-improving-women-s-health-in-vulnerable-populations
#1
Elena R Frati, Ester Fasoli, Marianna Martinelli, Daniela Colzani, Silvia Bianchi, Luciana Carnelli, Antonella Amendola, Pierfranco Olivani, Elisabetta Tanzi
BACKGROUND: Migrant women are one of the most vulnerable population to health problems and well-being. This study aimed at implementing a counseling and preventive strategy for sexually transmitted infections (STIs) in undocumented migrant women in Milan, Italy. METHODS: Women (ages 18-65) were enrolled at the NAGA Centre (2012-2013) and asked for a urine sample in order to carry out molecular detection of Human papillomavirus (HPV), Chlamydia trachomatis (Ct), Trichomonas vaginalis (Tv), Neisseria gonorrhoeae (Ng)-DNA...
June 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28629588/human-papillomavirus-and-its-testing-assays-cervical-cancer-screening-and-vaccination
#2
Yusheng Zhu, Yun Wang, Julie Hirschhorn, Kerry J Welsh, Zhen Zhao, Michelle R Davis, Sarah Feldman
Human papillomavirus (HPV) was found to be the causative agent for cervical cancer in the 1980s with almost 100% of cervical cancer cases testing positive for HPV. Since then, many studies have been conducted to elucidate the molecular basis of HPV, the mechanisms of carcinogenesis of the virus, and the risk factors for HPV infection. Traditionally, the Papanicolaou test was the primary screening method for cervical cancer. Because of the discovery and evolving understanding of the role of HPV in cervical dysplasia, HPV testing has been recommended as a new method for cervical cancer screening by major professional organizations including the American Cancer Society, American Society for Colposcopy and Cervical Pathology, and the American Society for Clinical Pathology...
2017: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/28627834/characteristics-and-distribution-of-penile-lesions-in-croatian-men-with-laboratory-confirmed-low-risk-and-high-risk-human-papillomavirus-hpv-infection
#3
Tomislav Mestrovic, Zora Profozic, Marijana Neuberg, Goran Kozina, Ivan Savic, Velimir Profozic
BACKGROUND: Studies that compare penoscopic evaluation with the results of molecular human papillomavirus (HPV) testing are scarce. This study assessed and compared both type and distribution of various penile lesions using penoscopy (peniscopy) technique in Croatian men with different, laboratory-confirmed HPV status. METHODS: From a large cohort of men attending an outpatient STD clinic for HPV testing, a total of 120 patients were randomly selected and grouped into those positive for low-risk HPV, high-risk HPV, both low-risk and highrisk HPV, and those with negative results...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28626810/human-papillomavirus-hpv-in-young-women-in-britain-population-based-evidence-of-the-effectiveness-of-the-bivalent-immunisation-programme-and-burden-of-quadrivalent-and-9-valent-vaccine-types
#4
Clare Tanton, David Mesher, Simon Beddows, Kate Soldan, Soazig Clifton, Kavita Panwar, Nigel Field, Catherine H Mercer, Anne M Johnson, Pam Sonnenberg
BACKGROUND: In 2008, the UK introduced an HPV immunisation programme in girls. Population-based prevalence estimates of bivalent (HPV-16/18), quadrivalent (HPV-6/11/16/18) and 9-valent (HPV-6/11/16/18/31/33/45/52/58) vaccine types, and comparison over time, are needed to monitor impact, evaluate effectiveness and guide decision-making on vaccination strategies. METHODS: The third National Survey of Sexual Attitudes and Lifestyles (Natsal-3) in 2010-12, tested urine for HPV from 2569 sexually-experienced women aged 16-44...
June 2017: Papillomavirus Research
https://www.readbyqxmd.com/read/28621317/p16-immunohistochemistry-in-oropharyngeal-squamous-cell-carcinoma-a-comparison-of-antibody-clones-using-patient-outcomes-and-high-risk-human-papillomavirus-rna-status
#5
Jeremy Shelton, Bibianna M Purgina, Nicole A Cipriani, William D Dupont, Dale Plummer, James S Lewis
High-risk human papillomavirus (HPV)-related oropharyngeal squamous cell carcinomas have a more favorable prognosis than HPV-negative ones. p16 immunohistochemistry has been recommended as a prognostic test in clinical practice. Several p16 antibodies are available, and their performance has not been directly compared. We evaluated three commercially available p16 antibody clones (E6H4, JC8 and G175-405) utilizing 199 cases of oropharyngeal squamous cell carcinoma from a tissue microarray, read by three pathologists with three different cutoffs for positivity: any staining, >50% and >75%...
June 16, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/28619772/cost-effectiveness-of-hpv-based-cervical-cancer-screening-in-the-public-health-system-in-nicaragua
#6
Nicole G Campos, Mercy Mvundura, Jose Jeronimo, Francesca Holme, Elisabeth Vodicka, Jane J Kim
OBJECTIVES: To evaluate the cost-effectiveness of human papillomavirus (HPV) DNA testing (versus Papanicolaou (Pap)-based screening) for cervical cancer screening in Nicaragua. DESIGN: A previously developed Monte Carlo simulation model of the natural history of HPV infection and cervical cancer was calibrated to epidemiological data from Nicaragua. Cost data inputs were derived using a micro-costing approach in Carazo, Chontales and Chinandega departments; test performance data were from a demonstration project in Masaya department...
June 15, 2017: BMJ Open
https://www.readbyqxmd.com/read/28614466/prevalence-of-high-risk-human-papillomavirus-among-women-in-two-english-speaking-caribbean-countries
#7
Glennis Andall-Brereton, Eulynis Brown, Sherian Slater, Yvette Holder, Silvana Luciani, Merle Lewis, Beryl Irons
Objective: To characterize high-risk human papillomavirus (HPV) infections in a sample of women in two small English-speaking Caribbean countries: Saint Kitts and Nevis and Saint Vincent and the Grenadines. Methods: Sexually active women ≥ 30 years old attending primary care health facilities participated in the study. Each participant had a gynecological examination, and two cervical specimens were collected: (1) a specimen for a Papanicolaou (Pap) test and (2) a sample of exfoliated cervical cells for HPV DNA testing, using the HPV High Risk Screen Real-TM (Sacace)...
June 8, 2017: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28610586/genotyping-of-high-risk-anal-human-papillomavirus-hpv-ion-torrent-next-generation-sequencing-vs-linear-array
#8
Rebecca G Nowak, Nicholas P Ambulos, Lisa M Schumaker, Trevor J Mathias, Ruth A White, Jennifer Troyer, David Wells, Manhattan E Charurat, Søren M Bentzen, Kevin J Cullen
BACKGROUND: Our next generation sequencing (NGS)-based human papillomavirus (HPV) genotyping assay showed a high degree of concordance with the Roche Linear Array (LA) with as little as 1.25 ng formalin-fixed paraffin-embedded-derived genomic DNA in head and neck and cervical cancer samples. This sensitive genotyping assay uses barcoded HPV PCR broad-spectrum general primers 5+/6+ (BSGP)5+/6+ applicable to population studies, but it's diagnostic performance has not been tested in cases with multiple concurrent HPV infections...
June 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/28608796/the-natural-history-of-oral-human-papillomavirus-in-young-costa-rican-women
#9
Daniel C Beachler, Krystle A Lang Kuhs, Linda Struijk, John Schussler, Rolando Herrero, Carolina Porras, Allan Hildesheim, Bernal Cortes, Joshua Sampson, Wim Quint, Paula Gonzalez, Aimée R Kreimer
BACKGROUND: Oral human papillomavirus (HPV) infection and related oropharyngeal cancer are uncommon in lower-income countries, particularly compared to HPV-associated cervical cancer. However, little is known about the natural history of oral HPV in less-developed settings and how it compares to the natural history of cervical HPV. METHODS: Three hundred fifty women aged 22 to 33 years from the Costa Rica Vaccine Trial provided exfoliated cells from the cervical and oral regions at 2 visits 2 years apart...
July 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28608795/human-papillomavirus-vaccination-among-young-men-who-have-sex-with-men-and-transgender-women-in-2-us-cities-2012-2014
#10
Pamina M Gorbach, Ryan Cook, Beau Gratzer, Thomas Collins, Adam Parrish, Janell Moore, Peter R Kerndt, Richard A Crosby, Lauri E Markowitz, Elissa Meites
BACKGROUND: Since 2011, in the United States, quadrivalent human papillomavirus (HPV) vaccine has been recommended for boys aged 11 to 12 years, men through age 21, and men who have sex with men (MSM) through age 26. We assessed HPV vaccination coverage and factors associated with vaccination among young MSM (YMSM) and transgender women (TGW) in 2 cities. METHODS: During 2012-2014, 808 YMSM and TGW aged 18 to 26 years reported vaccination status in a self-administered computerized questionnaire at 3 sexually transmitted disease (STD) clinics in Los Angeles and Chicago...
July 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28607383/isolation-detection-and-quantification-of-cancer-biomarkers-in-hpv-associated-malignancies
#11
Hakan Inan, Shuqi Wang, Fatih Inci, Murat Baday, Richard Zangar, Sailaja Kesiraju, Karen S Anderson, Brian T Cunningham, Utkan Demirci
Human Papillomavirus (HPV) infection has been recognized as the main etiologic factor in the development of various cancers including penile, vulva, oropharyngeal and cervical cancers. In the development of cancer, persistent HPV infections induce E6 and E7 oncoproteins, which promote cell proliferation and carcinogenesis resulting elevated levels of host antibodies (e.g., anti-HPV16 E7 antibody). Currently, these cancers are clinically diagnosed using invasive biopsy-based tests, which are performed only in centralized labs by experienced clinical staff using time-consuming and expensive tools and technologies...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28604561/breast-milk-is-a-potential-vehicle-for-human-papillomavirus-transmission-to-oral-mucosa-of-the-spouse
#12
Karolina Louvanto, Marja Sarkola, Marjut Rintala, Kari Syrjänen, Seija Grenman, Stina Syrjänen
BACKGROUND: Human papillomavirus (HPV) DNA has been detected in breast milk, but its origin has remained obscure. The aim of the study was to analyze the prevalence and persistence of HPV in breast milk in the Finnish Family HPV cohort study. The association of breast milk HPV positivity with the family members' oral HPV status was evaluated. METHODS: We included 308 families to the study where the mother was breast feeding her offspring. Mothers collected the milk samples manually at day 3, and at months 2, 6 and 12...
July 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28604063/vaginal-self-sampling-as-a-diagnosis-tool-in-low-income-countries-and-potential-applications-for-exploring-the-infectious-causes-of-miscarriage
#13
Jean-Christophe Lagier, Nafissatou Diagne, Florence Fenollar, Catherine Tamalet, Cheikh Sokhna, Didier Raoult
Gynecological health is a challenge in low-income countries. Personal opposition to perineal examination has been overcome by the use of vaginal self-sampling. Here, we review the use of this procedure notably in low-income countries and the main infectious causes of miscarriage. Vaginal self-sampling was mainly used for human papillomavirus detection but also to detect microorganisms causing sexually transmitted infections or bacterial vaginosis. 58 studies have been performed in low-resource countries, mainly studies performed to detect human papillomavirus in urban and peri-urban areas and demonstrating excellent acceptability...
June 12, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28589730/variation-in-genital-human-papillomavirus-infection-prevalence-and-vaccination-coverage-among-men-and-women-in-the-usa
#14
Jasmine J Han, Christopher M Tarney, John Song
Human papillomavirus (HPV) is the most common sexually transmitted infection that causes majority of anogenital and oropharyngeal cancers. Prophylactic HPV vaccine is available for the primary prevention of cancer and HPV transmission. Here, we are going to discuss the variation of HPV prevalence, HPV vaccination coverage and potential risk factors of men and women, retrieved from the cross-sectional study of the National Health Nutrition Examination Survey, a representative sample of noninstitutionalized, civilian residents in the USA...
June 7, 2017: Future Oncology
https://www.readbyqxmd.com/read/28587376/clinical-progress-of-human-papillomavirus-genotypes-and-their-persistent-infection-in-subjects-with-atypical-squamous-cells-of-undetermined-significance-cytology-statistical-and-latent-dirichlet-allocation-analysis
#15
Yee Suk Kim, Sungin Lee, Nansu Zong, Jimin Kahng
The present study aimed to investigate differences in prognosis based on human papillomavirus (HPV) infection, persistent infection and genotype variations for patients exhibiting atypical squamous cells of undetermined significance (ASCUS) in their initial Papanicolaou (PAP) test results. A latent Dirichlet allocation (LDA)-based tool was developed that may offer a facilitated means of communication to be employed during patient-doctor consultations. The present study assessed 491 patients (139 HPV-positive and 352 HPV-negative cases) with a PAP test result of ASCUS with a follow-up period ≥2 years...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28586650/e6-proteins-of-%C3%AE-and-%C3%AE-cutaneous-hpv-types-differ-in-their-ability-to-potentiate-wnt-signaling
#16
Sophia Sominsky, Naama Shterzer, Anna Jackman, Beny Shapiro, Abraham Yaniv, Levana Sherman
We recently showed that E6 protein of human papillomavirus (HPV) 16, a mucosal high-risk α-PV type, can potentiate Wnt/β-catenin/TCF signaling. Here we investigated the transcriptional activities of E6 proteins of cutaneous HPV types from the β and α genera. Results from reporter-gene assays showed that similar to HPV16 E6, E6 of HPV10, a cutaneous α-HPV type that is prevalent in skin warts, efficiently enhances and stimulates Wnt/β-catenin/TCF transcription. HPV10 E6 also effectively elevated the expression levels of β-catenin and promoted its nuclear accumulation...
June 3, 2017: Virology
https://www.readbyqxmd.com/read/28584747/expression-of-human-papillomavirus-dna-and-p53-polymorphisms-through-polymerase-chain-reaction-in-normal-mucosa-and-oral-leukoplakia-individuals-with-deleterious-oral-habits
#17
Atmakuri Shanmukha Ramya, Sumit Majumdar, T Mahesh Babu, Divya Uppala, Baratam Srinivas, Ayyagari Kameswara Rao
CONTEXT: Oral leukoplakia (OL) is a well-recognized precancerous lesion with various etiological factors. Most commonly deleterious oral habits such as tobacco smoking and viral etiologies mainly human papillomavirus (HPV) play an important role. p53 polymorphisms mostly homozygous Arginine (Arg) allele has a greater risk of degradation by HPV. Hence, HPV infection and p53 polymorphisms may act as synergistic factors for increased the risk of malignant transformation in oral precancerous lesions...
April 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28584635/interactions-between-polymorphisms-in-the-3-untranslated-region-of-the-cyclin-dependent-kinase-6-gene-and-the-human-papillomavirus-infection-and-risk-of-cervical-precancerous-lesions
#18
Xingguang Ye, Lipeng Jing, Xingming Zhong, Di Xiao, Meiling Ou, Congcong Guo, Guang Yang, Chunxia Jing, Xiangcai Wei
The human papillomavirus (HPV) infection is essential for the development of cervical cancer and its precursor lesions. However, only certain persistently infected individuals develop cervical cancer. Cyclin-dependent kinase 6 (CDK6) is a critical regulatory cancer-associated gene in the cell cycle and in tumorigenesis. Single nucleotide polymorphisms (SNPs) in microRNA sites in the 3'-untranslated region (UTR) of target genes may result in target gene expression level changes and susceptibility to diseases, including cancer...
June 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28583102/analysis-of-epidemiological-trends-in-human-papillomavirus-infection-among-gynaecological-outpatients-in-hangzhou-china-2011-2015
#19
Lili Qian, Yu Zhang, Dawei Cui, Bin Lou, Yimin Chen, Ying Yu, Yonglin Liu, Yu Chen
BACKGROUND: HPV infection is the major pathogenic factor underlying cervical cancer and precancerous lesions. The cervical HPV infection rates in gynaecological outpatients from Hangzhou, China, were studied in the period from January 2011 to December 2015. METHODS: Exfoliated cervical cells were harvested from gynaecological outpatients in Hangzhou from January 2011 to December 2015. Twenty-one HPV subtypes were detected using flow-through hybridization. The HPV infection rates in various disease groups were compared using the Chi-square test...
June 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28583086/independent-prognostic-role-of-human-papillomavirus-genotype-in-cervical-cancer
#20
Dong Hang, Meiqun Jia, Hongxia Ma, Jing Zhou, Xiaoshuang Feng, Zhangyan Lyu, Jian Yin, Hong Cui, Yin Yin, Guangfu Jin, Zhibin Hu, Hongbing Shen, Kai Zhang, Ni Li, Min Dai
BACKGROUND: Although the correlation of HPV genotype with cervical precursor lesions and invasive cancer has been confirmed, the role of HPV genotype in cervical cancer prognosis is less conclusive. This study aims to systematically investigate the independent prognostic role of HPV genotype in cervical cancer. METHODS: A total of 306 eligible patients provided cervical cell specimens for HPV genotyping before therapy and had a median follow-up time of 54 months after diagnosis...
June 5, 2017: BMC Infectious Diseases
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