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https://www.readbyqxmd.com/read/29731751/unique-action-of-interleukin-18-on-t-cells-and-other-immune-cells
#1
REVIEW
Kenji Nakanishi
Interleukin (IL)-18 was originally discovered as a factor that enhances interferon (IFN)-γ production by anti-CD3-stimulated Th1 cells, particularly in association with IL-12. IL-12 is a cytokine that induces development of Th1 cells. IL-18 cannot induce Th1 cell development, but has the capacity to activate established Th1 cells to produce IFN-γ in the presence of IL-12. Thus, IL-18 is regarded as a proinflammatory cytokine that facilitates type 1 responses. However, in the absence of IL-12 but presence of IL-2, IL-18 stimulates natural killer cells, NKT cells, and even established Th1 cells to produce IL-3, IL-9, and IL-13...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29724998/il-3-but-not-monomeric-ige-regulates-fc%C3%AE%C2%B5ri-levels-and-cell-survival-in-primary-human-basophils
#2
Fabian Zellweger, Patrick Buschor, Gabriel Hobi, Daniel Brigger, Clemens Andreas Dahinden, Peter Matthias Villiger, Alexander Eggel
Binding of allergen-specific IgE to its primary receptor FcεRI on basophils and mast cells represents a central event in the development of allergic diseases. The high-affinity interaction between IgE and FcεRI results in permanent sensitization of these allergic effector cells and critically regulates their release of pro-inflammatory mediators upon IgE cross-linking by allergens. In addition, binding of monomeric IgE has been reported to actively regulate FcεRI surface levels and promote survival of mast cells in the absence of allergen through the induction of autocrine cytokine secretion including interleukin-3 (IL-3)...
May 3, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29721337/influence-of-seasonality-and-circulating-cytokines-on-serial-quantiferon-discordances
#3
Marsha L Griffin, Saroochi Agarwal, Melissa Zhu Murphy, Larry D Teeter, Edward A Graviss
Objectives. An 18-month prospective study serially tested healthcare workers (HCWs) for tuberculosis infection (TBI) and reported discordant QuantiFERON Gold In-Tube® (QFT) results in some participants. The purpose of the current study was to investigate whether the interferon-gamma (IFN- γ ) measured by QFT in discordant individuals could be influenced by other circulating cytokines that vary seasonally at the time of phlebotomy. Methods. The CDC funded TBESC Task Order 18 (TO18) project to assess the use of Interferon Gamma Release Assays (IGRAs), T-SPOT...
2018: Tuberculosis Research and Treatment
https://www.readbyqxmd.com/read/29720239/a-subset-of-mobilized-human-hematopoietic-stem-cells-express-germ-layer-lineage-genes-which-can-be-modulated-by-culture-conditions
#4
Tapas Makar, Vamshi K Nimmagadda, Poornachander R Guda, Brian Hampton, Weiliang Huang, Maureen A Kane, Paul S Fishman, Bernard Pessac, Christopher T Bever, David Trisler
BACKGROUND: Adult bone marrow contains stem cells that replenish the myeloid and lymphoid lineages. A subset of human and mouse CD34+ bone marrow stem cells can be propagated in culture to autonomously express embryonic stem cell genes and embryonic germ layer lineage genes. The current study was undertaken to determine whether these CD34+ stem cells could be obtained from human blood, whether gene expression could be modulated by culture conditions and whether the cells produce insulin...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29708814/protective-effect-of-the-c-mpl-agonist-romiplostim-on-megakaryocytopoiesis-of-human-cd34-hematopoietic-progenitor-cells-exposed-to-ionizing-radiation
#5
Satoru Monzen, Shunta Kimura, Masaru Yamaguchi, Ikuo Kashiwakura
A thrombopoiesis-stimulating protein, the myeloproliferative leukemia virus protooncogene (Mpl) ligand romiplostim (RP), is currently approved as a therapeutic agent for idiopathic thrombocytopenic purpura in many countries. Although the action of the initial MPL ligand thrombopoietin (TPO) on human megakaryocytic regeneration from irradiated human hematopoietic stem cells has been examined, there are few reports on the action of RP. In the present study, freshly prepared nonirradiated and 2-Gy X-irradiated human CD34 positive (CD34+ ) cells from placental umbilical cord blood were cultured with a combination of RP and various cytokines...
April 30, 2018: Journal of Interferon & Cytokine Research
https://www.readbyqxmd.com/read/29706072/proteomic-and-phospho-proteomic-changes-induced-by-prolonged-activation-of-human-eosinophils-with-il-3
#6
Stephane Esnault, Alexander S Hebert, Nizar N Jarjour, Joshua J Coon, Deane F Mosher
Purified human eosinophils treated for 18-24 h with IL-3 adopt a unique activated phenotype marked by increased reactivity to aggregated immunoglobulin-G (IgG). To characterize this phenotype, we quantified protein abundance and phosphorylation by multi-plexed isobaric labeling combined with high-resolution mass spectrometry. Purified blood eosinophils of five individuals were treated with IL-3 or no cytokine for 20 hours, and comparative data were obtained on abundance of 5385 proteins and phosphorylation at 7330 sites...
April 29, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29686375/requirement-of-gsk-3-for-puma-induction-upon-loss-of-pro-survival-pi3k-signaling
#7
Florian Schubert, Juliane Rapp, Prisca Brauns-Schubert, Lisa Schlicher, Kerstin Stock, Manuela Wissler, Martina Weiß, Céline Charvet, Christoph Borner, Ulrich Maurer
Growth factor withdrawal induces rapid apoptosis via mitochondrial outer membrane permeabilization. We had previously observed that cell death of IL-3-dependent Ba/F3 cells, induced by removal of the growth factor, required the activity of the kinase GSK-3. Employing CRISPR/Cas9-mediated gene knockout, we aimed to identify pro-apoptotic GSK-3 regulated factors in this process. Knockout of either Puma or Bim demonstrated that the induction of Puma, but not Bim, was crucial for apoptosis induced by IL-3 deprivation...
April 23, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29685196/il-2-il-3-interplay-mediates-growth-of-cd25-positive-acute-myeloid-leukemia-cells
#8
Kazunori Nakase, Kenkichi Kita, Naoyuki Katayama
Cell surface interleukin-2 receptor α-chain (IL-2Rα, CD25) expression is currently recognized to be a strong predictor for poor prognosis in patients with acute myeloid leukemia (AML). However, it is still unknown that the reason why CD25 positive AML patients have a dismal clinical outcome. CD25 positive AML cells are generally unresponsive to IL-2, but strongly respond to IL-3. The levels of IL-3Rα on these AML cells are very high and directly proportional to the CD25 levels. T-lymphocytes produce IL-3 in response to stimuli including IL-2-mediated activation...
June 2018: Medical Hypotheses
https://www.readbyqxmd.com/read/29684429/simultaneous-in-vitro-generation-of-human-cd34-derived-dendritic-cells-and-mast-cells-from-non-mobilized-peripheral-blood-mononuclear-cells
#9
Pavla Taborska, Jirina Bartunkova, Daniel Smrz
Dendritic cells (DCs) and mast cells (MCs) are key players of the immune system, often coming in close proximity in peripheral tissues. The interplay of these cells is, however, still poorly understood especially with regards to human cells. The reason for that is the absence of a well established in vitro human cell-based study system that would allow a simultaneous preparation of both cell types. In this study, we show a method for simultaneous generation of DCs and MCs from CD34+ stem cell progenitors that were isolated from the non-adherent fraction of non-mobilized peripheral blood mononuclear cells of healthy donors...
April 20, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29677365/age-related-changes-to-human-tear-composition
#10
Alessandra Micera, Antonio Di Zazzo, Graziana Esposito, Rosa Longo, William Foulsham, Roberto Sacco, Roberto Sgrulletta, Stefano Bonini
Purpose: We characterize age-associated alterations in the expression of inflammatory mediators and tissue remodeling factors in human tears. Methods: A total of 75 consecutive volunteers (32 male/44 female; 19-93 years) underwent clinical assessment of ocular surface status, ocular surface disease index (OSDI) grading and tear sampling. The volunteers were categorized into three groups: young (18-40 years), middle-aged (41-60 years), and old (>60 years). Total protein profiles and chip-based protein array evaluations were conducted to investigate the expression of 60 potential candidates, including pro-/anti-inflammatory mediators and tissue remodeling factors...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29676074/immune-characterization-of-bone-marrow-derived-models-of-mucosal-and-connective-tissue-mast-cells
#11
Sara Benedé, Evan Cody, Charuta Agashe, M Cecilia Berin
PURPOSE: It is well appreciated that mast cells (MCs) demonstrate tissue-specific imprinting, with different biochemical and functional properties between connective tissue MCs (CTMCs) and mucosal MCs (MMCs). Although in vitro systems have been developed to model these different subsets, there has been limited investigation into the functional characteristics of the 2 major MC subsets. Here, we report the immunologic characterization of 2 MCs subsets developed in vitro from bone marrow progenitors modeling MMCs and CTMCs...
May 2018: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/29671586/a-zirconium-photosensitizer-with-a-long-lived-excited-state-mechanistic-insight-into-photo-induced-single-electron-transfer
#12
Yu Zhang, Tia S Lee, Jeffrey L Petersen, Carsten Milsmann
Time-resolved emission spectroscopy for the luminescent zirconium complex Zr(Me PDP)2 (Me PDP = 2,6-bis(5-methyl-3-phenyl-1H-pyrrol-2-yl)pyridine) revealed a long-lived excited state with a lifetime τ = 325 ± 10 μs. Computational studies using time-dependent density functional theory (TD-DFT) were conducted to identify the nature of the luminescent excited state as a mixed triplet intraligand/ligand-to-metal charge transfer (3 IL/3 LMCT) state. Stern-Volmer experiments showed a strong dependence of the quenching rate on the redox potential of the quencher indicating photo-induced single electron transfer (SET) as the quenching pathway...
April 19, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29572350/gain-of-function-mutations-in-granulocyte-colony-stimulating-factor-receptor-csf3r-reveal-distinct-mechanisms-of-csf3r-activation
#13
Haijiao Zhang, Cody Coblentz, Kevin Watanabe-Smith, Sophie Means, Jasmine Means, Julia E Maxson, Jeffrey W Tyner
Granulocyte colony-stimulating factor (G-CSF or CSF3) and its receptor CSF3R regulate granulopoiesis, neutrophil function, and hematopoietic stem cell mobilization. Recent studies have uncovered an oncogenic role of mutations in the CSF3R gene in many hematologic malignancies. To find additional CSF3R mutations that give rise to cell transformation, we performed a cellular transformation assay in which murine interleukin 3 (IL-3)-dependent Ba/F3 cells were transduced with wildtype CSF3R plasmid and screened for spontaneous growth in the absence of IL-3...
March 23, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29554544/interleukin-3-symptoms-and-cognitive-deficits-in-first-episode-drug-na%C3%A3-ve-and-chronic-medicated-schizophrenia
#14
Mei-Hong Xiu, Dong Wang, Song Chen, Xiang-Dong Du, Da-Chun Chen, Nan Chen, Yue-Chan Wang, Guangzhong Yin, Yingyang Zhang, Yun-Long Tan, Raymond Y Cho, Jair C Soares, Xiang-Yang Zhang
Previous studies consistently showed that IL-3 signaling may be involved in the pathophysiology of schizophrenia. However, investigations of associations between IL-3 and the neurocognitive impairments are lacking, including the study of how this may vary with stage of illness. We recruited 45 first-episode drug-naïve (FE-Sz), 35 chronic medicated schizophrenia (Ch-Sz) and 40 healthy controls (HC) and examined the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) and serum IL-3. Altered serum IL-3 levels were found in both patient groups compared with HC group (both p < 0...
March 6, 2018: Psychiatry Research
https://www.readbyqxmd.com/read/29553501/proliferation-and-differentiation-of-murine-myeloid-precursor-32d-g-csf-r-cells
#15
Polina Zjablovskaja, Petr Danek, Miroslava Kardosova, Meritxell Alberich-Jorda
Understanding of the hematopoietic stem and progenitor cell biology has important implications for regenerative medicine and the treatment of hematological pathologies. Despite the most relevant data that can be acquired using in vivo models or primary cultures, the low abundance of hematopoietic stem and progenitor cells considerably restricts the pool of suitable techniques for their investigation. Therefore, the use of cell lines allows sufficient production of biological material for the performance of screenings or assays that require large cell numbers...
February 21, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29552867/mechanisms-of-allergic-diseases-in-otorhinolaryngology
#16
E Ridolo, I Martignago, S Masieri
Allergic Rhinitis (AR) is an IgE-mediated hypersensitivity disease caused by inhalation of an allergen to which the patients is sensitized. Etiopathogenesis of AR comprises a sensitization phase, an immediate phase and a late phase. In the sensitization phase, inhaled allergens are processed in peptides and come into contact with the nasal mucosa cells. Antigen-Presenting Cells (APCs), especially represented by Dendritic Cells (DCs), capture them through the interaction with their own MHC class II complexes and migrate to lymph nodes...
January 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29545893/the-investigation-of-immunomodulatory-activities-of-gloeostereum-incaratum-polysaccharides-in-cyclophosphamide-induced-immunosuppression-mice
#17
Di Wang, Qian Li, Yidi Qu, Mengya Wang, Lanzhou Li, Yang Liu, Yu Li
Gloeostereum incarnatum , a precious edible mushroom, displays anti-bacterial and anti-inflammatory activities; however, its immunomodulatory effect has not been studied yet. The present study aimed to investigate whether polysaccharide compositions of G. incarnatum polysaccharides (GIPS) possess immunomodulatory and immuno-enhancing effects in a Cyclophosphamide monohydrate (CTX)-induced BALB/c mice model. The 28-day GIPS administration at doses of 0.1, 0.3 and 0.9 g/kg remarkably reversed the bodyweight loss, increased the thymic index and promoted T lymphocyte proliferation in CTX-induced immunosuppressed mice...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29543810/identification-of-plasma-biomarkers-for-discrimination-between-tuberculosis-infection-disease-and-pulmonary-non-tuberculosis-disease
#18
Marco Pio La Manna, Valentina Orlando, Paolo Li Donni, Guido Sireci, Paola Di Carlo, Antonio Cascio, Francesco Dieli, Nadia Caccamo
We used the Luminex Bead Array Multiplex Immunoassay to measure cytokines, chemokines and growth factors responses to the same antigens used for RD1-based Interferon γ Release Assay (IGRA) test. Seventy-nine individuals, 27 active TB, 32 latent infection subsets, 20 individuals derivative purified protein (PPD) negative (subjects that do not have any indurative cutaneous reaction after 72 hrs of intradermal injection of PPD) and with other pulmonary disease were retrospectively studied. Forty-eight analytes were evaluated by Luminex Assay in plasma obtained from whole blood stimulated cells...
2018: PloS One
https://www.readbyqxmd.com/read/29533646/integrated-biosensor-for-rapid-and-point-of-care-sepsis-diagnosis
#19
Jouha Min, Maria Nothing, Ben Coble, Hui Zheng, Jongmin Park, Hyungsoon Im, Georg F Weber, Cesar M Castro, Filip K Swirski, Ralph Weissleder, Hakho Lee
Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages i) the newly-found pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis, and ii) a hybrid magneto-electrochemical sensor for IL-3 detection...
March 13, 2018: ACS Nano
https://www.readbyqxmd.com/read/29523595/interleukin-3-stimulates-matrix-metalloproteinase-12-production-from-macrophages-promoting-thoracic-aortic-aneurysm-dissection
#20
Chang Liu, Congcong Zhang, Lixin Jia, Boya Chen, Luxin Liu, Jie Sun, Wenmei Zhang, Bin You, Yulin Li, Ping Li, Jie Du
Thoracic aortic aneurysm and dissection (TAAD) is due to degeneration of the aorta and causes a high mortality rate, while molecular mechanisms for the development of TAAD are still not completely understood. In this study, 3-aminopropionitrile (BAPN) treatment was used to induce TAAD mouse model. Through transcriptome analysis, we found the expression levels of genes associated with interleukin-3 (IL-3) signaling pathway were upregulated during TAAD development in mouse, which were validated by real-time PCR...
March 9, 2018: Clinical Science (1979-)
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