Tuğba Semerci Sevimli, Murat Sevimli, Aynaz Ghorbani, Varol Şahintürk, Emilia Qomi Ekenel, Tuğba Ertem, Bahar Demir Cevizlidere, Burcugül Altuğ, Özlem Tomsuk, Onur Uysal, Sibel Güneş Bağış, Hüseyin Avci, Fatih Çemrek, Zarifa Ahmadova
Targeting lung cancer stem cells (LC-SCs) for metastasis may be an effective strategy against lung cancer. This study is the first on epithelial-mesenchymal transition (EMT) properties of boric acid (BA) in LC-SCs. LC-SCs were isolated using the magnetic cell sorting (MACS) method. Tumor-sphere formation and flow cytometry confirmed CSC phenotype. The cytotoxic effect of BA was measured by MTT analysis, and the effect of BA on EMT was examined by migration analysis. The expression levels of ZEB1, SNAIL1, ITGA5, CDH1, ITGB1, VIM, COL1A1, and LAMA5 genes were analyzed by RT-qPCR...
March 27, 2024: Naunyn-Schmiedeberg's Archives of Pharmacology