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0PN embryo transfer

Ming Li, Shengli Lin, Yuan Chen, Jinliang Zhu, Ping Liu, Jie Qiao
OBJECTIVE: To determine the value of transferring embryos formed from nonpronuclear (0PN) zygotes. DESIGN: A case-control study. SETTING: Not applicable. PATIENT(S): The current study was a retrospective analysis of embryo transfers of just 0PN embryos using fresh cleavage-stage embryos (0PN cleavage fresh), frozen-thawed cleavage-stage 0PN embryos (0PN cleavage frozen), and frozen 0PN blastocyst-stage embryos (0PN blast frozen)...
September 2015: Fertility and Sterility
Fang Wang, Hui-Juan Kong, Quan-Cheng Kan, Ju-Yan Liang, Fang Zhao, Ai-Hong Bai, Peng-Fen Li, Ying-Pu Sun
In recent years, applications of stem cells have already involved in all domains of life science and biomedicine. People try to establish human embryonic stem cell lines (hESCs) in order to carry out hESC-related studies. In this study, we explored what embryos are conducive to the establishment of hESCs. The discarded embryos from in vitro fertilization-embryo transfer (IVF-ET) cycles were sequentially incubated into blastocysts, and then the inner cell mass (ICM) was isolated and incubated in the mixed feeder layer...
December 2012: Journal of Cellular Biochemistry
Junping Cheng, Li Huang, Bing He, Fenghua Lu, Xiaoli Wang, Zhengsan Wu, Deshun Shi
To determine whether the age of the female patient, oocyte maturation stage, pronuclei, embryonic quality and/or cycle outcome are related to the intensity of the zona pellucida birefringence (ZPB) in oocytes during IVF procedures, a retrospective study was conducted on 214 IVF cycles using a polarisation imaging system. A negative correlation was found between ZPB intensity and the age of female patients (r = -0.44; P < 0.0001). For oocytes at different maturation stages, the ZPB score was higher at the germinal vesicle stage (20...
2010: Reproduction, Fertility, and Development
Nicole Noyes, M Elizabeth Fino, Lewis Krey, Caroline McCaffrey, Alexis Adler, James Grifo
This study assessed 1908 embryos, including those with abnormal numbers of pronuclei, in IVF cycles from July 2001 to December 2006 in which preimplantation genetic screening (PGS) was performed on day 3 post-retrieval and 'euploid' embryos transferred the following day. PGS-intracytoplasmic sperm injection and PGS-translocation cycles were excluded. At 18 h post-insemination, the zygote distribution was 19% 0PN, 4% 1PN, 69% 2PN and 8% 3PN. No pregnancy occurred following 0PN or 1PN embryo transfers. A retrospective, blinded morphological ranking of all embryos on day 3 was performed and the results compared with PGS; no 0PN or 1PN embryo would have been chosen for transfer based on morphological superiority alone...
December 2008: Reproductive Biomedicine Online
R O Burney, J Gebhardt, Y Shu, B Behr, L M Westphal
PURPOSE: To report the case of a patient undergoing in vitro fertilization (IVF) in which a non-pronuclear (0PN) oocyte resulted in a normal pregnancy. METHODS: A 36-year-old woman underwent an IVF-embryo transfer treatment cycle. RESULTS: Four oocytes were retrieved for insemination by IVF. Examination for fertilization revealed two polypronuclearpolygynic and two non-pronuclear oocytes. The non-pronuclear oocytes were observed further for development...
2008: Clinical and Experimental Obstetrics & Gynecology
Ibrahim Hammoud, Fran├žois Vialard, Pierre Casasnovas, Gilles Lefebvre, Danielle Vauthier-Brouzes, Catherine Poirot
OBJECTIVE: To evaluate the pertinence of kinetic observations of embryonic development 25 hours after IVF, with a view to optimizing the choice of embryos for transfer. DESIGN: Retrospective study of 471 cycles with embryo transfer. SETTING: University Hospital. INTERVENTION(S): Observations of changes in zygotes, in the 25th hour after insemination (25 h PI). MAIN OUTCOME MEASURE(S): Identification of three stages of development: two cells (2C), loss of the pronuclei (in syngamy; 0PN) and persistence of the two pronuclei (2PN)...
September 2008: Fertility and Sterility
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