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https://www.readbyqxmd.com/read/29388691/comparison-of-dual-trigger-with-combination-gnrh-agonist-and-hcg-versus-hcg-only-trigger-of-oocyte-maturation-for-normal-ovarian-responders
#1
Xingyu Zhou, Pingping Guo, Xin Chen, Desheng Ye, Yudong Liu, Shiling Chen
OBJECTIVE: To investigate whether dual triggering of oocyte maturation with a gonadotropin-releasing hormone (GnRH) agonist and standard dose of human chorionic gonadotropin (hCG) can improve clinical outcomes for normal ovarian responders in GnRH antagonist cycles. METHODS: The present retrospective cohort study included women aged up to 40 years with normal ovarian response who underwent in vitro fertilization and/or intracytoplasmic sperm injection under the GnRH antagonist protocol at Nanfang Hospital, China, between January 1 and December 31, 2015...
February 1, 2018: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/29364218/production-of-genetically-engineered-golden-syrian-hamsters-by-pronuclear-injection-of-the-crispr-cas9-complex
#2
Rong Li, Jinxin Miao, Zhiqiang Fan, SeokHwan Song, Il-Keun Kong, Yaohe Wang, Zhongde Wang
The pronuclear (PN) injection technique was first established in mice to introduce foreign genetic materials into the pronuclei of one-cell stage embryos. The introduced genetic material may integrate into the embryonic genome and generate transgenic animals with foreign genetic information following transfer of the injected embryos to foster mothers. Following the success in mice, PN injection has been applied successfully in many other animal species. Recently, PN injection has been successfully employed to introduce reagents with gene-modifying activities, such as the CRISPR/Cas9 system, to achieve site-specific genetic modifications in several laboratory and farm animal species...
January 9, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29297236/cleavage-stage-embryo-micromanipulation-in-the-clinical-setting
#3
Iman Halvaei, Shahin Ghazali, Stefania A Nottola, Mohammad Ali Khalili
Embryo micromanipulation was developed after introduction of microinjection to overcome infertility. Embryo micromanipulation may be performed at any embryo stage from pronuclear to blastocyst. The technique started out as basic and turned out to be increasingly more complex. Embryo micromanipulation at the cleavage-stage includes a wide range of techniques, from opening the zona pellucida in order to improve the chance of implantation, to removing detrimental components from the embryo to enhance embryo development or blastomeres for preimplantation genetic diagnosis and embryo splitting...
January 3, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29296266/male-pronuclear-formation-and-embryo-development-following-intracytoplasmic-injection-of-ovine-pretreated-sperm
#4
Abolfazl Shirazi, Arefeh Golestanfar, Masomeh Bashiri, Ebrahim Ahmadi, Naser Shams-Esfandabadi
Background: Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some compounds. Methods: In experiment 1, sperm were treated with either 2-mercaptoethanol (2ME), glutathione (GSH), or DTT (dithiothreitol) in combination with Heparin (Hep)...
January 2018: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29288975/fertilizing-capacity-of-vitrified-epididymal-sperm-from-iberian-ibex-capra-pyrenaica
#5
J Pradieé, M J Sánchez-Calabuig, C Castaño, E O'Brien, M C Esteso, P Beltrán-Breña, V Maillo, J Santiago-Moreno, D Rizos
In this study, we successfully described for the first time a vitrification of epididymal Iberian ibex spermatozoa. Spermatozoa from epididymis were obtained from 15 Iberian ibex. The right epididymis' semen sample was vitrified and the left one was frozen. After thawing/warming, samples were selected by density gradient. Sperm characteristics from each treatment were evaluated. To test the spermatozoa fertilization ability, heterologous IVF was carried out using bovine oocytes. Despite of the observation of a decrease of about 40% for motility sperm between pre-freezing and post-thawing (75...
December 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29259470/potential-of-zygotes-to-produce-live-births-can-be-identified-by-the-size-of-the-male-and-female-pronuclei-just-before-their-membranes-break-down
#6
Junko Otsuki, Toshiroh Iwasaki, Yuta Tsuji, Yuya Katada, Haruka Sato, Yukari Tsutsumi, Kanae Hatano, Kohyu Furuhashi, Yukiko Matsumoto, Shoji Kokeguchi, Masahide Shiotani
Aim: To determine whether there are differences in size between the male and female pronuclei immediately before the pronuclear membrane breakdown (PNMBD) and to evaluate whether pronuclear size differences influence normal birth rates. Methods: Time-lapse photography was used to measure the size of each pronucleus, while the outcome of 71 frozen-thawed single blastocyst transfers in patients receiving hormone therapy was analyzed retrospectively. The pronuclear measurements were made 4 hours before the PNMBD, corresponding to 16-20 hours after insemination or intracytoplasmic sperm injection, and immediately before the PNMBD...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29249046/generation-of-transgenic-goats-by-pronuclear-microinjection-a-retrospective-analysis-of-a-commercial-operation-1995-2012
#7
W Gavin, S Blash, N Buzzell, D Pollock, L Chen, N Hawkins, J Howe, K Miner, J Pollock, C Porter, M Schofield, Y Echelard, H Meade
Production of transgenic founder goats involves introducing and stably integrating an engineered piece of DNA into the genome of the animal. At LFB USA, the ultimate use of these transgenic goats is for the production of recombinant human protein therapeutics in the milk of these dairy animals. The transgene or construct typically links a milk protein specific promoter sequence, the coding sequence for the gene of interest, and the necessary downstream regulatory sequences thereby directing expression of the recombinant protein in the milk during the lactation period...
December 16, 2017: Transgenic Research
https://www.readbyqxmd.com/read/29243141/comparison-of-the-development-of-human-embryos-cultured-in-either-an-embryoscope-or-benchtop-incubator
#8
R Sciorio, J K Thong, S J Pickering
PURPOSE: In this current study, our main goal was to establish that EmbryoScope incubation environment is comparable to standard incubation. METHODS: The development of sibling human zygotes was compared after culture in either a benchtop incubator (SI) or an EmbryoScope time-lapse incubator (ES). Between May 2015 to April 2016, a total of 581 normally fertilized 2PN, pronuclear-stage embryos, from 47 patients were allocated to culture in either a benchtop incubator (SI) or an EmbryoScope incubator (ES)...
December 15, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29175537/vitrification-of-human-pronuclear-oocytes-by-direct-plunging-into-cooling-agent-non-sterile-liquid-nitrogen-vs-sterile-liquid-air
#9
Vladimir Isachenko, Plamen Todorov, Akerke Seisenbayeva, Yerzhan Toishibekov, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Dolores Foth, Markus Merzenich
In fact, a full sterilization of commercially-produced liquid nitrogen contaminated with different pathogens is not possible. The aim of this study was to compare the viability of human pronuclear oocytes subjected to cooling by direct submerging of open carrier in liquid nitrogen versus submerging in clean liquid air (aseptic system). One- and three-pronuclei stage embryos (n = 444) were cryopreserved by direct plunging into liquid nitrogen (vitrified) in ethylene glycol (15%), dimethylsulphoxide (15%) and 0...
November 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#10
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
January 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29118980/sirt1-dependent-modulation-of-methylation-and-acetylation-of-histone-h3-on-lysine-9-h3k9-in-the-zygotic-pronuclei-improves-porcine-embryo-development
#11
Katerina Adamkova, Young-Joo Yi, Jaroslav Petr, Tereza Zalmanova, Kristyna Hoskova, Pavla Jelinkova, Jiri Moravec, Milena Kralickova, Miriam Sutovsky, Peter Sutovsky, Jan Nevoral
Background: The histone code is an established epigenetic regulator of early embryonic development in mammals. The lysine residue K9 of histone H3 (H3K9) is a prime target of SIRT1, a member of NAD(+)-dependent histone deacetylase family of enzymes targeting both histone and non-histone substrates. At present, little is known about SIRT1-modulation of H3K9 in zygotic pronuclei and its association with the success of preimplantation embryo development. Therefore, we evaluated the effect of SIRT1 activity on H3K9 methylation and acetylation in porcine zygotes and the significance of H3K9 modifications for early embryonic development...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29074360/perivitelline-threads-in-cleavage-stage-human-embryos-observations-using-time-lapse-imaging
#12
Louise Kellam, Laura M Pastorelli, Angel M Bastida, Amy Senkbeil, Sue Montgomery, Simon Fishel, Alison Campbell
Time-lapse imaging of the human preimplantation embryo in vitro has revealed a transient phenomenon involving the appearance of perivitelline threads, commonly observed at the two-cell stage. These threads span the perivitelline space, arising at the specific area where the cytoplasmic membrane contacts the zona pellucida, before any perivitelline space is formed. The threads persist as the cytoplasmic membrane retracts from the zona pellucida to form the first cleavage furrow. In this observational report, these structures and their incidence are described...
September 28, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29034763/pronuclear-pattern-does-not-predict-morphokinetics-behavior-in-human-embryos
#13
Azita Faramarzi, Mohammad Ali Khalili, Marjan Omidi, Azam Agha-Rahimi, Fatemeh Taheri
The purpose was to investigate the correlation between pronuclei (PN) morphology and morphokinetic behaviors of derived embryos with time lapse monitoring (TLM) in assisted reproduction setting. Over time, PN morphology from PN appearance (PNA) to PN fading (PNF), PNF according to size, contact, number and position of nuclear precursor bodies (NPBs) within each PN and morphokinetics variables, including absolute time points, relative timing parameters, cleavage patterns and arrest rate, were evaluated using TLM...
October 16, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29034232/maternally-contributed-folate-receptor-1-is-expressed-in-ovarian-follicles-and-contributes-to-preimplantation-development
#14
Trine Strandgaard, Solveig Foder, Anders Heuck, Erik Ernst, Morten S Nielsen, Karin Lykke-Hartmann
Folates have been shown to play a crucial role for proper development of the embryo as folate deficiency has been associated with reduced developmental capacity such as increased risk of fetal neural tube defects and spontanous abortion. Transcripts encoding the reduced folate carrier RFC1 (SLC19A1 protein) and the high-affinity folate receptor FOLR1 are expressed in oocytes and preimplantation embryos, respectively. In this study, we observed maternally contributed FOLR1 protein during mouse and human ovarian follicle development, and 2-cell mouse embryos...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28955073/comparison-of-embryo-morphokinetics-following-intracytoplasmic-sperm-injection-in-smoker-and-non-smoker-couples-are-the-results-different
#15
Ahmet Salvarci, Ali Sami Gurbuz, Sukru Uzman, Melek Kaya, Huseyin Gorkemli
OBJECTIVE: To assess early embryo development via time-lapse in smokers and non-smokers. METHODS: The retrospective study was conducted at Novafertil IVF centers in Konya, Turkey and comprised oocytes of both smoker and non-smoker couples subjected to in vitro fertilisation / introcytoplasmic sperm injection from 2012 to 2015. Age, basal follicle-stimulating hormone, number of stimulation days, amount of gonadotropin used, number of metaphase II oocytes, number of embryos transferred and pregnancy, abortus and clinical pregnancy rates were noted...
October 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28886319/morphokinetic-analysis-of-human-embryo-development-and-its-relationship-to-the-female-age-a-retrospective-time-lapse-imaging-study
#16
N Akhter, M Shahab
Time-lapse imaging technique has provided embryologists with a unique chance of studying the embryo morphokineticsto select the most viable embryos for implantation in the field of IVF (In vitro fertilization).The conventional method of morphological evaluations has proved that maternal age affects the human embryo quality. This retrospective study carried out at Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan mainly focuses on the effect of female age on human embryo morphokinetics. A total number of 200 patients undergoing ICSI treatment cycles at the clinic were selected for the study and divided into five age groups (< 26, 26-30, 31-35, 36-40, and > 40 years)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28855524/rnai-combining-sleeping-beauty-transposon-system-inhibits-ex-vivo-expression-of-foot-and-mouth-disease-virus-vp1-in-transgenic-sheep-cells
#17
Shoulong Deng, Guangdong Li, Kun Yu, Xiuzhi Tian, Feng Wang, Wenting Li, Wuqi Jiang, Pengyun Ji, Hongbing Han, Juncai Fu, Xiaosheng Zhang, Jinlong Zhang, Yixun Liu, Zhengxing Lian, Guoshi Liu
Foot and mouth disease, which is induced by the foot and mouth disease virus (FMDV), takes its toll on the cloven-hoofed domestic animals. The VP1 gene in FMDV genome encodes the viral capsid, a vital element for FMDV replication. Sleeping Beauty (SB) is an active DNA-transposon system responsible for genetic transformation and insertional mutagenesis in vertebrates. In this study, a conserved VP1-shRNA which specifically targets the ovine FMDV-VP1 gene was constructed and combined with SB transposase and transposon...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809019/lentiviral-mediated-transgenesis-in-songbirds
#18
Wan-Chun Liu, Marian Hruska-Plochan, Atsushi Miyanohara
Transgenesis involves the insertion of an exogenous gene into an animal's genome, which allows the identification of the expressed phenotypes in brain function or behavior. Lentiviral-mediated transgenesis offers unique transduction potency making it possible to deliver and stably integrate transgenes into a wide variety of dividing and nondividing cells. The ability to establish long-term expression of such transgenes allows their use for transgenesis which is especially useful in organisms lacking quality pluripotent stem cell lines and which is otherwise difficult to produce via traditional pronuclear microinjection, such as songbirds...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28795048/evaluation-of-human-embryo-development-in-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-fertilized-oocytes-a-time-lapse-study
#19
Hyung Jun Kim, Hye Jin Yoon, Jung Mi Jang, Won Don Lee, San Hyun Yoon, Jin Ho Lim
OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28765300/pronuclear-transplantation-in-the-mouse-embryo
#20
James McGrath, Davor Solter, Jeff Mann
This protocol describes an example of complete zygote enucleation and transplantation of male and female pronuclei; however, single pronuclei can also be removed and transplanted. In this method, pronuclei are removed without penetrating the plasma membrane of the zygote. Instead, they are withdrawn individually or together into a membrane-bound karyoplast that can then be fused with a recipient enucleated zygote using inactivated Sendai virus or electrofusion. Preincubation of the embryos in the presence of the cytoskeletal inhibitors cytochalasin B and colcemid is critical for the survival of the embryos during this microsurgical procedure...
August 1, 2017: Cold Spring Harbor Protocols
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