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https://www.readbyqxmd.com/read/29564316/human-chorionic-gonadotropin-administered-natural-cycle-versus-spontaneous-ovulatory-cycle-in-patients-undergoing-two-pronuclear-zygote-frozen-thawed-embryo-transfer
#1
You-Jung Lee, Chung-Hoon Kim, Do-Young Kim, Jun-Woo Ahn, Sung-Hoon Kim, Hee-Dong Chae, Byung-Moon Kang
Objective: To compare human chorionic gonadotropin (HCG)-administered natural cycle with spontaneous ovulatory cycle in patients undergoing frozen-thawed embryo transfer (FTET) in natural cycles. Methods: In this retrospective cohort study, we analyzed the clinical outcome of a total of 166 consecutive FTET cycles that were performed in either natural cycle controlled by HCG for ovulation triggering (HCG group, n=110) or natural cycle with spontaneous ovulation (control group, n=56) in 166 infertile patients between January 2009 and November 2013...
March 2018: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/29542120/the-effect-of-premature-luteinizing-hormone-increases-among-high-ovarian-responders-undergoing-a-gonadotropin-releasing-hormone-antagonist-ovarian-stimulation-protocol
#2
Yudi Geng, Qiaohong Lai, Yang Xun, Lei Jin
OBJECTIVE: To explore factors causing a premature rise in luteinizing hormone among high ovarian responders undergoing the gonadotropin-releasing hormone (GnRH) antagonist ovarian stimulation protocol. METHODS: The present retrospective study included healthy women undergoing fresh cycles using a fixed GnRH antagonist protocol with a predicted high response and antral follicle count (AFC) of at least 15 at the Reproductive Medicine Center of Tongji Hospital, China, between January 1 and December 31, 2016...
March 15, 2018: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/29503398/peroxiredoxin-as-a-functional-endogenous-antioxidant-enzyme-in-pronuclei-of-mouse-zygotes
#3
Kohtaro Morita, Mikiko Tokoro, Yuki Hatanaka, Chika Higuchi, Haruka Ikegami, Kouhei Nagai, Masayuki Anzai, Hiromi Kato, Tasuku Mitani, Yoshitomo Taguchi, Kazuo Yamagata, Yoshihiko Hosoi, Kei Miyamoto, Kazuya Matsumoto
Antioxidant mechanisms to adequately moderate levels of endogenous reactive oxygen species (ROS) are important for oocytes and embryos to obtain and maintain developmental competence, respectively. Immediately after fertilization, ROS levels in zygotes are elevated but the antioxidant mechanisms during the maternal-to-zygotic transition (MZT) are not well understood. First, we identified peroxiredoxin 1 (PRDX1) and PRDX2 by proteomics analysis as two of the most abundant endogenous antioxidant enzymes eliminating hydrogen peroxide (H2 O2 )...
March 2, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29485024/overexpression-of-microrna-29b-decreases-expression-of-dna-methyltransferases-and-improves-quality-of-the-blastocysts-derived-from-somatic-cell-nuclear-transfer-in-cattle
#4
Shuang Liang, Zheng-Wen Nie, Jing Guo, Ying-Jie Niu, Kyung-Tae Shin, Sun A Ock, Xiang-Shun Cui
MicroRNA (miR)-29b plays a crucial role during somatic cell reprogramming. The aim of the current study was to explore the effects of miR-29b on the developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos, as well as the underlying mechanisms of action. The expression level of miR-29b was lower in bovine SCNT embryos at the pronuclear, 8-cell, and blastocyst stages compared with in vitro fertilized embryos. In addition, miR-29b regulates the expression of DNA methyltransferases (Dnmt3a/3b and Dnmt1) in bovine SCNT embryos...
February 2018: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29479641/correlation-between-the-pronucleus-size-and-the-potential-for-human-single-pronucleus-zygotes-to-develop-into-blastocysts-1pn-zygotes-with-large-pronuclei-can-expect-an-embryo-development-to-the-blastocyst-stage-that-is-similar-to-the-development-of-2pn-zygotes
#5
Eri Araki, Fumiaki Itoi, Hiroyuki Honnma, Yukiko Asano, Hisanori Oguri, Kazuyo Nishikawa
PURPOSE: In this study, we examined the correlation between pronucleus size and the potential for human single pronucleus (1PN) zygotes to develop into blastocysts after IVF and ICSI. METHODS: This study included 112 patients who underwent a total of 112 cycles of IVF/ICSI. To evaluate embryo development, 1PN zygotes were compared with 2PN zygotes in the same IVF/ICSI cycle (control cycles) using time-lapse live embryo imaging. To assess the potential for blastocyst formation, cutoff values for pronuclear area and diameter were established through receiver operating characteristic curve analysis, after which 1PN zygotes were classified based on those cutoff values...
February 26, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29477154/review-sperm-oocyte-interactions-and-their-implications-for-bull-fertility-with-emphasis-on-the-ubiquitin-proteasome-system
#6
P Sutovsky
Fertilization is an intricate cascade of events that irreversibly alter the participating male and female gamete and ultimately lead to the union of paternal and maternal genomes in the zygote. Fertilization starts with sperm capacitation within the oviductal sperm reservoir, followed by gamete recognition, sperm-zona pellucida interactions and sperm-oolemma adhesion and fusion, followed by sperm incorporation, oocyte activation, pronuclear development and embryo cleavage. At fertilization, bull spermatozoon loses its acrosome and plasma membrane components and contributes chromosomes, centriole, perinuclear theca proteins and regulatory RNAs to the zygote...
February 26, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29475998/chromatin-characterization-in-xenopus-laevis-cell-free-egg-extracts-and-embryos
#7
Wei-Lin Wang, Takashi Onikubo, David Shechter
Xenopus laevis development is marked by accelerated cell division solely supported by the proteins maternally deposited in the egg. Oocytes mature to eggs with concomitant transcriptional silencing. The unique maternal chromatin state contributing to this silencing and subsequent zygotic activation is likely established by histone posttranslational modifications and histone variants. Therefore, tools for understanding the nature and function of maternal and embryonic histones are essential to deciphering mechanisms of regulation of development, chromatin assembly, and transcription...
February 23, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29459681/embryonic-defects-induced-by-maternal-obesity-in-mice-derive-from-stella-insufficiency-in-oocytes
#8
Longsen Han, Chao Ren, Ling Li, Xiaoyan Li, Juan Ge, Haichao Wang, Yi-Liang Miao, Xuejiang Guo, Kelle H Moley, Wenjie Shu, Qiang Wang
Maternal obesity can impair embryo development and offspring health, yet the mechanisms responsible remain poorly understood. In a high-fat diet (HFD)-based female mouse model of obesity, we identified a marked reduction of Stella (also known as DPPA3 or PGC7) protein in oocytes. Starting with this clue, we found that the establishment of pronuclear epigenetic asymmetry in zygotes from obese mice was severely disrupted, inducing the accumulation of maternal 5-hydroxymethylcytosine modifications and DNA lesions...
March 2018: Nature Genetics
https://www.readbyqxmd.com/read/29458909/effect-of-morphokinetics-and-morphological-dynamics-of-cleavage-stage-on-embryo-developmental-potential-a-time-lapse-study
#9
Shiao-Hsuan Yang, Cheng-Hsuan Wu, Yu-Ching Chen, Chueh-Ko Yang, Tsui-Huang Wu, Pei-Chi Chen, Horng-Der Tsai
OBJECTIVE: Using a non-invasive method to select the most competent embryo is essential in in vitro fertilization (IVF). Since the beginning of clinical application of time-lapse technology, several studies have proposed models using the time-lapse imaging system for predicting the IVF outcome. This study used both morphokinetic and morphological dynamic parameters to select embryos with the highest developmental potential. MATERIALS AND METHODS: A total of 23 intracytoplasmic sperm injection treatment cycles with 138 fertilized oocytes were included in this study...
February 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29388691/comparison-of-dual-trigger-with-combination-gnrh-agonist-and-hcg-versus-hcg-only-trigger-of-oocyte-maturation-for-normal-ovarian-responders
#10
Xingyu Zhou, Pingping Guo, Xin Chen, Desheng Ye, Yudong Liu, Shiling Chen
OBJECTIVE: To investigate whether dual triggering of oocyte maturation with a gonadotropin-releasing hormone (GnRH) agonist and standard dose of human chorionic gonadotropin (hCG) can improve clinical outcomes for normal ovarian responders in GnRH antagonist cycles. METHODS: The present retrospective cohort study included women aged up to 40 years with normal ovarian response who underwent in vitro fertilization and/or intracytoplasmic sperm injection under the GnRH antagonist protocol at Nanfang Hospital, China, between January 1 and December 31, 2015...
February 1, 2018: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/29364218/production-of-genetically-engineered-golden-syrian-hamsters-by-pronuclear-injection-of-the-crispr-cas9-complex
#11
Rong Li, Jinxin Miao, Zhiqiang Fan, SeokHwan Song, Il-Keun Kong, Yaohe Wang, Zhongde Wang
The pronuclear (PN) injection technique was first established in mice to introduce foreign genetic materials into the pronuclei of one-cell stage embryos. The introduced genetic material may integrate into the embryonic genome and generate transgenic animals with foreign genetic information following transfer of the injected embryos to foster mothers. Following the success in mice, PN injection has been applied successfully in many other animal species. Recently, PN injection has been successfully employed to introduce reagents with gene-modifying activities, such as the CRISPR/Cas9 system, to achieve site-specific genetic modifications in several laboratory and farm animal species...
January 9, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29297236/cleavage-stage-embryo-micromanipulation-in-the-clinical-setting
#12
Iman Halvaei, Shahin Ghazali, Stefania A Nottola, Mohammad Ali Khalili
Embryo micromanipulation was developed after introduction of microinjection to overcome infertility. Embryo micromanipulation may be performed at any embryo stage from pronuclear to blastocyst. The technique started out as basic and turned out to be increasingly more complex. Embryo micromanipulation at the cleavage-stage includes a wide range of techniques, from opening the zona pellucida in order to improve the chance of implantation, to removing detrimental components from the embryo to enhance embryo development or blastomeres for preimplantation genetic diagnosis and embryo splitting...
January 3, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29296266/male-pronuclear-formation-and-embryo-development-following-intracytoplasmic-injection-of-ovine-pretreated-sperm
#13
Abolfazl Shirazi, Arefeh Golestanfar, Masomeh Bashiri, Ebrahim Ahmadi, Naser Shams-Esfandabadi
Background: Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some compounds. Methods: In experiment 1, sperm were treated with either 2-mercaptoethanol (2ME), glutathione (GSH), or DTT (dithiothreitol) in combination with Heparin (Hep)...
January 2018: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/29288975/fertilizing-capacity-of-vitrified-epididymal-sperm-from-iberian-ibex-capra-pyrenaica
#14
J Pradieé, M J Sánchez-Calabuig, C Castaño, E O'Brien, M C Esteso, P Beltrán-Breña, V Maillo, J Santiago-Moreno, D Rizos
In this study, we successfully described for the first time a vitrification of epididymal Iberian ibex spermatozoa. Spermatozoa from epididymis were obtained from 15 Iberian ibex. The right epididymis' semen sample was vitrified and the left one was frozen. After thawing/warming, samples were selected by density gradient. Sperm characteristics from each treatment were evaluated. To test the spermatozoa fertilization ability, heterologous IVF was carried out using bovine oocytes. Despite of the observation of a decrease of about 40% for motility sperm between pre-freezing and post-thawing (75...
March 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29259470/potential-of-zygotes-to-produce-live-births-can-be-identified-by-the-size-of-the-male-and-female-pronuclei-just-before-their-membranes-break-down
#15
Junko Otsuki, Toshiroh Iwasaki, Yuta Tsuji, Yuya Katada, Haruka Sato, Yukari Tsutsumi, Kanae Hatano, Kohyu Furuhashi, Yukiko Matsumoto, Shoji Kokeguchi, Masahide Shiotani
Aim: To determine whether there are differences in size between the male and female pronuclei immediately before the pronuclear membrane breakdown (PNMBD) and to evaluate whether pronuclear size differences influence normal birth rates. Methods: Time-lapse photography was used to measure the size of each pronucleus, while the outcome of 71 frozen-thawed single blastocyst transfers in patients receiving hormone therapy was analyzed retrospectively. The pronuclear measurements were made 4 hours before the PNMBD, corresponding to 16-20 hours after insemination or intracytoplasmic sperm injection, and immediately before the PNMBD...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29249046/generation-of-transgenic-goats-by-pronuclear-microinjection-a-retrospective-analysis-of-a-commercial-operation-1995-2012
#16
W Gavin, S Blash, N Buzzell, D Pollock, L Chen, N Hawkins, J Howe, K Miner, J Pollock, C Porter, M Schofield, Y Echelard, H Meade
Production of transgenic founder goats involves introducing and stably integrating an engineered piece of DNA into the genome of the animal. At LFB USA, the ultimate use of these transgenic goats is for the production of recombinant human protein therapeutics in the milk of these dairy animals. The transgene or construct typically links a milk protein specific promoter sequence, the coding sequence for the gene of interest, and the necessary downstream regulatory sequences thereby directing expression of the recombinant protein in the milk during the lactation period...
February 2018: Transgenic Research
https://www.readbyqxmd.com/read/29243141/comparison-of-the-development-of-human-embryos-cultured-in-either-an-embryoscope-or-benchtop-incubator
#17
R Sciorio, J K Thong, S J Pickering
PURPOSE: In this current study, our main goal was to establish that EmbryoScope incubation environment is comparable to standard incubation. METHODS: The development of sibling human zygotes was compared after culture in either a benchtop incubator (SI) or an EmbryoScope time-lapse incubator (ES). Between May 2015 to April 2016, a total of 581 normally fertilized 2PN, pronuclear-stage embryos, from 47 patients were allocated to culture in either a benchtop incubator (SI) or an EmbryoScope incubator (ES)...
December 15, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29175537/vitrification-of-human-pronuclear-oocytes-by-direct-plunging-into-cooling-agent-non-sterile-liquid-nitrogen-vs-sterile-liquid-air
#18
Vladimir Isachenko, Plamen Todorov, Akerke Seisenbayeva, Yerzhan Toishibekov, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Dolores Foth, Markus Merzenich
In fact, a full sterilization of commercially-produced liquid nitrogen contaminated with different pathogens is not possible. The aim of this study was to compare the viability of human pronuclear oocytes subjected to cooling by direct submerging of open carrier in liquid nitrogen versus submerging in clean liquid air (aseptic system). One- and three-pronuclei stage embryos (n = 444) were cryopreserved by direct plunging into liquid nitrogen (vitrified) in ethylene glycol (15%), dimethylsulphoxide (15%) and 0...
February 2018: Cryobiology
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#19
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
January 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29118980/sirt1-dependent-modulation-of-methylation-and-acetylation-of-histone-h3-on-lysine-9-h3k9-in-the-zygotic-pronuclei-improves-porcine-embryo-development
#20
Katerina Adamkova, Young-Joo Yi, Jaroslav Petr, Tereza Zalmanova, Kristyna Hoskova, Pavla Jelinkova, Jiri Moravec, Milena Kralickova, Miriam Sutovsky, Peter Sutovsky, Jan Nevoral
Background: The histone code is an established epigenetic regulator of early embryonic development in mammals. The lysine residue K9 of histone H3 (H3K9) is a prime target of SIRT1, a member of NAD+ -dependent histone deacetylase family of enzymes targeting both histone and non-histone substrates. At present, little is known about SIRT1-modulation of H3K9 in zygotic pronuclei and its association with the success of preimplantation embryo development. Therefore, we evaluated the effect of SIRT1 activity on H3K9 methylation and acetylation in porcine zygotes and the significance of H3K9 modifications for early embryonic development...
2017: Journal of Animal Science and Biotechnology
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