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glucose and corneal cross link

FangJun Bao, ManLi Deng, XiaoBo Zheng, LinNa Li, YiPing Zhao, Si Cao, AYong Yu, QinMei Wang, JinHai Huang, Ahmed Elsheikh
To investigate the effects of diabetes on the biomechanical behavior of cornea in alloxan-induced diabetic rabbits. Diabetes mellitus (DM) was induced in 20 rabbits using alloxan, while another 20 age- and weight-matched non-diabetic rabbits served as controls. Eyes were enucleated after 8 weeks of inducing diabetes and the whole cornea was removed with a 3 mm wide scleral ring and tested under inflation conditions with an internal pressure range of 2.0-30.0 mmHg to determine their stress-strain behavior using an inverse analysis process...
August 2017: Experimental Eye Research
Jui-Yang Lai, Hsiao-Yun Cheng, David Hui-Kang Ma
Hyaluronic acid (HA) is a linear polysaccharide naturally found in the eye and therefore is one of the most promising biomaterials for corneal endothelial regenerative medicine. This study reports, for the first time, the development of overrun-processed porous HA hydrogels for corneal endothelial cell (CEC) sheet transplantation and tissue engineering applications. The hydrogel carriers were characterized to examine their structures and functions. Evaluations of carbodiimide cross-linked air-dried and freeze-dried HA samples were conducted simultaneously for comparison...
2015: PloS One
George D Kymionis, Dimitrios A Liakopoulos, Michael A Grentzelos, Konstantinos I Tsoulnaras, Efstathios T Detorakis, Béatrice Cochener, Miltiadis K Tsilimbaris
PURPOSE: To evaluate the effect of a regenerative agent (RGTA) [Cacicol20-poly(carboxymethyl glucose sulfate); OTR3, Paris, France] on corneal reepithelialization and pain after corneal cross-linking (CXL) for keratoconus. METHODS: In this prospective comparative (contralateral) clinical study, patients with bilateral progressive keratoconus underwent CXL treatment. The corneal epithelium during CXL was removed using transepithelial phototherapeutic keratectomy (Cretan protocol)...
August 2015: Cornea
Na Li, Xiujun Peng, Zhengjun Fan
Corneal collagen cross-linking with UVA-riboflavin is currently the only method for preventing the progression of keratoconus from the pathological perspective. Topical application of a direct cross-linking agent is now attracting widespread attention in clinical settings. This article reviews the research progress in the application of indirect or direct cross-linking agents (e.g., riboflavin, glucose, ribose, glutaraldehyde, formaldehyde, glyceraldehyde, short chain aliphatic β-nitro alcohol, and genipin) in the treatment of corneal diseases and analyzes the cross-linking efficacy, toxicity, and merits and disadvantages of each cross-linking agent, providing clinical information for further studies...
June 2014: Eye Science
Yuyu Long, Li Ren, Jiaming Wang, Min Chen, Yang Liu, Binqian Liu, Yingjun Wang, Jian Ge
In order to investigate the feasibility of the modified chitosan-gelatin crosslinked membrane (MC-Gel) and chitosan-gelatin crosslinked membrane (CS-Gel) to be a potential biomaterial for corneal regeneration, we evaluated their physicochemical properties and intraocular biocompatibility in this study. White light transmission and permeability of these membranes were detected. Results showed that white light transmission of both membranes was above 90% at 500 nm, which was similar to that of human cornea. The glucose, tryptophan and NaCl permeability of MC-Gel membrane and CS-Gel membrane was better than or similar to those of human cornea...
February 2013: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
Jui-Yang Lai, David Hui-Kang Ma, Meng-Heng Lai, Ya-Ting Li, Ren-Jie Chang, Li-Mei Chen
Cell sheet-mediated tissue regeneration is a promising approach for corneal reconstruction. However, the fragility of bioengineered corneal endothelial cell (CEC) monolayers allows us to take advantage of cross-linked porous gelatin hydrogels as cell sheet carriers for intraocular delivery. The aim of this study was to further investigate the effects of biopolymer concentrations (5-15 wt%) on the characteristic and safety of hydrogel discs fabricated by a simple stirring process combined with freeze-drying method...
2013: PloS One
Jui-Yang Lai
A nanoscale modification strategy that can incorporate chondroitin sulfate (CS) into the cross-linked porous gelatin materials has previously been proposed to give superior performance for designed corneal keratocyte scaffolds. The purpose of this work was to further investigate the influence of carbodiimide chemistry on the characteristics and biofunctionalities of gelatin/CS scaffolds treated with varying N-hydroxysuccinimide (NHS)/1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) molar ratios (0-1) at a constant EDC concentration of 10 mM...
2013: International Journal of Molecular Sciences
Jui-Yang Lai, Ya-Ting Li, Ching-Hsien Cho, Ting-Chun Yu
Recent studies reflect the importance of using naturally occurring biopolymers as three-dimensional corneal keratocyte scaffolds and suggest that the porous structure of gelatin materials may play an important role in controlling nutrient uptake. In the current study, the authors further consider the application of carbodiimide cross-linked porous gelatin as an alternative to collagen for corneal stromal tissue engineering. The authors developed corneal keratocyte scaffolds by nanoscale modification of porous gelatin materials with chondroitin sulfate (CS) using carbodiimide chemistry...
2012: International Journal of Nanomedicine
Yu Xia, Chen Tao, Chuanqing Zhou, Qiushi Ren
PURPOSE: To investigate the possible synergistic effect of ultraviolet A (UVA)/riboflavin and glucose on corneal collagen cross-linking (CXL) by means of the mechanical properties and thermal shrinkage temperatures. METHODS: Forty rabbits were divided into four groups: control group; UVA/glucose group; UVA/riboflavin group; and UVA/riboflavin/glucose group. After the treatments, the mechanical properties and thermal shrinkage temperatures of the corneal strips were analyzed to determine the CXL effects of corneal collagen induced by different treatments...
March 2011: Journal of Refractive Surgery
Jui-Yang Lai, Ya-Ting Li
An efficient carrier for corneal endothelial cell therapy should deliver and retain the cell sheet transplants at the site of injury without causing adverse effects. Here we introduced a simple stirring process combined with freeze-drying (SFD1) method for the development of gelatin hydrogels with enlarged pore structure that can improve the aqueous humor circulation. Samples fabricated by air-drying (AD) or freeze-drying method were used for comparison. After cross-linking with 1 mM 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC), the discs were investigated to assess their functionality...
May 10, 2010: Biomacromolecules
H C Kim, Y J Cho, C W Ahn, K S Park, J C Kim, J S Nam, Y S Im, J E Lee, S C Lee, H K Lee
AIMS: Low serum nerve growth factor (NGF) levels have been reported in patients with diabetic peripheral neuropathy (DPN), but the role of NGF in the development of neuropathy is unclear. Thus, we investigated the associations of serum NGF level and NGF receptor activity with the presence and severity of DPN. METHODS: One hundred and thirty-six patients with Type 2 diabetes were included in this cross-sectional study. Serum NGF levels were measured by ELISA. Expressions of NGF receptors (TrkA and p75(NTR)) were measured by immunohistochemical staining...
December 2009: Diabetic Medicine: a Journal of the British Diabetic Association
Mark A Babizhayev, Philip Micans, Andrea Guiotto, Anne Kasus-Jacobi
The antioxidant activity of L-carnosine (beta-alanyl-L-histidine, bioactivated in ocular tissues) versus N-acetylcarnosine (N-acetyl-beta-alanyl-L-histidine, ocular-targeted small dipeptide molecules) was studied in aqueous solution and in a lipid environment, employing liposomes as a model of lipid membranes. Reactive oxygen species (ROS) were generated by an iron/ascorbate promoter system for induction of lipid peroxidation (LPO). L-carnosine, which is stabilized from enzymatic hydrolysis, operates as a universal aldehyde and ROS scavenger in both aqueous and lipid environments and is effective at preventing ROS-induced damage to biomolecules...
November 2009: American Journal of Therapeutics
Gregor Wollensak, Henning Aurich, Christopher Wirbelauer, Duy-Thoai Pham
BACKGROUND: Collagen cross-linking of the cornea has been shown by us to have an antiedematous effect in the cornea. The aim of the present study was to examine if this effect can be used for the treatment of bullous keratopathy. METHODS: This clinical interventional case series included 3 patients (3 eyes) with bullous keratopathy due to pseudophakia, corneal transplant rejection, and Fuchs' endothelial dystrophy. After dehydration for 1 day using 40% glucose, the central 8 mm of the cornea were abraded and cross-linked with the photosensitizer riboflavin and UVA (370 nm, 3 mW/cm(2)) for 30 min...
2009: Ophthalmic Research
Mark A Babizhayev, Andrea Guiotto, Anne Kasus-Jacobi
AIMS: In human diabetes, the deleterious effects of chronic hyperglycemia are the result of excessive nonenzymatic modification of proteins and phospholipids by glucose and its by-products leading to the formation of irreversible oxidized, aromatic, and fluorescent ligands known as advanced glycation end products. This glycation process has been associated with deleterious health effects. The present invention provides the potent inhibitors of protein glycation and AGEs formation, which are particularly advantageous for eyedrop delivery in the prevention and treatment of diabetes- and age-related pathologies...
January 2009: Journal of Drug Targeting
Mehrdad Rafat, Fengfu Li, Per Fagerholm, Neil S Lagali, Mitchell A Watsky, Rejean Munger, Takeshi Matsuura, May Griffith
Implantable biomaterials that mimic the extracellular matrix (ECM) in key physical and physiological functions require components and microarchitectures that are carefully designed to maintain the correct balance between biofunctional and physical properties. Our goal was to develop hybrid polymer networks (HPN) that combine the bioactive features of natural materials and physical characteristics of synthetic ones to achieve synergy between the desirable mechanical properties of some components with the biological compatibility and physiological relevance of others...
October 2008: Biomaterials
Ronald R Krueger, Jerome C Ramos-Esteban
PURPOSE: To determine how changes in corneal elasticity/stiffness might influence intraocular pressure (IOP) readings in diabetic patients. METHODS: A systematic review of the literature was performed to understand the conflicting relationship between diabetes, elevated IOP, and glaucoma progression. RESULTS: Diabetic patients have been found to have statistically significant higher IOP readings in some population-based studies compared to non-diabetics...
January 2007: Journal of Refractive Surgery
X Duan, H Sheardown
Generation 2 polypropyleneimine octaamine dendrimers were used to generate highly crosslinked collagen with mechanical properties that would make it appropriate for use as a corneal tissue-engineering scaffold. Crosslinking of a highly concentrated collagen solution (2-4%) was effected using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC). The multifunctional dendrimers were introduced as novel multifunctional crosslinkers after the activation of the carboxylic acid groups of glutamic and aspartic acid residues in collagen...
September 2006: Biomaterials
F Li, M Griffith, Z Li, S Tanodekaew, H Sheardown, M Hakim, D J Carlsson
Collagen hydrogel matrices with high optical clarity have been developed from collagen I, cross-linked with a copolymer based on N-isopropylacrylamide, acrylic acid and acryloxysuccinimide. The controlled reaction of collagen amine groups with this copolymer under neutral pH and aqueous conditions gave robust, optically clear hydrogels and prevented the excessive collagen fibrillogenesis that can lead to collagen opacity. These sterile, non-cytotoxic hydrogels allowed epithelial cell overgrowth and both stromal cell and nerve neurite ingrowth from the host tissue...
June 2005: Biomaterials
K V Chace, R Carubelli, R E Nordquist
Incubation of corneal collagen type I with glucose in the presence of transition metal ions (copper, iron) results in the formation of collagen aggregates insoluble in 6 M urea, and in 2% sodium dodecyl sulfate + 5% beta-mercaptoethanol. The reaction is mediated by hydrogen peroxide and transition metals since it is inhibited by catalase and by the chelating agent diethylenetriaminepentaacetic acid. Comparative studies showed that copper is more efficient than iron and that the reaction proceeds more rapidly with ribose than with glucose...
August 1, 1991: Archives of Biochemistry and Biophysics
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