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https://www.readbyqxmd.com/read/28827626/ergot-alkaloids-contribute-to-virulence-in-an-insect-model-of-invasive-aspergillosis
#1
Daniel G Panaccione, Stephanie L Arnold
Neosartorya fumigata (Aspergillus fumigatus) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a branch of the ergot alkaloid pathway called fumigaclavines. The biosynthesis of the N. fumigata ergot alkaloids and their relation to those produced by alternate pathway branches in fungi from the plant-inhabiting Clavicipitaceae have been well-characterised, but the potential role of these alkaloids in animal pathogenesis has not been studied extensively...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28772258/aspergilli-with-neosartorya-type-ascospores-heat-resistance-and-effect-of-sugar-concentration-on-growth-and-spoilage-incidence-in-berry-products
#2
Elettra Berni, Roberta Tranquillini, Nicoletta Scaramuzza, Andrea Brutti, Valentina Bernini
This study focused on four different heat resistant aspergilli: two strains of Aspergillus hiratsukae (≡Neosartorya hiratsukae), one strain of Aspergillus neoglaber (≡Neosartorya glabra), and one strain of Aspergillus thermomutatus (≡Neosartorya pseudofischeri), all isolated from spoiled pasteurized products. Their heat-resistance, the sugar concentration limiting their germination and growth in berry-based media, and a possible relation between the contamination levels of the raw materials used and the spoilage incidence in strawberry jams were assessed...
July 13, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28710744/cloning-and-characterization-of-f3pyc-gene-encoding-pyruvate-carboxylase-in-aspergillus-flavus-strain-f3
#3
Sadia Qayyum, Ibrar Khan, Zulfiqar Ahmad Bhatti, Changsheng Peng
Pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and L-malic acid. These organic acids play very important role for biological remediation of heavy metals. In this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (F3PYC) from heavy metal resistant indigenous fungal isolate Aspergillus flavus (F3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a calculated molecular weight of 131...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28620689/silencing-of-a-second-dimethylallyltryptophan-synthase-of-penicillium-roqueforti-reveals-a-novel-clavine-alkaloid-gene-cluster
#4
Ángeles Fernández-Bodega, Rubén Álvarez-Álvarez, Paloma Liras, Juan F Martín
Penicillium roqueforti produces several prenylated indole alkaloids, including roquefortine C and clavine alkaloids. The first step in the biosynthesis of roquefortine C is the prenylation of tryptophan-derived dipeptides by a dimethylallyltryptophan synthase, specific for roquefortine biosynthesis (roquefortine prenyltransferase). A second dimethylallyltryptophan synthase, DmaW2, different from the roquefortine prenyltransferase, has been studied in this article. Silencing the gene encoding this second dimethylallyltryptophan synthase, dmaW2, proved that inactivation of this gene does not prevent the production of roquefortine C, but suppresses the formation of other indole alkaloids...
August 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28512317/structural-determinants-of-neosartorya-fischeri-antifungal-protein-nfap-for-folding-stability-and-antifungal-activity
#5
László Galgóczy, Attila Borics, Máté Virágh, Hargita Ficze, Györgyi Váradi, Zoltán Kele, Florentine Marx
The recent global challenges to prevent and treat fungal infections strongly demand for the development of new antifungal strategies. The structurally very similar cysteine-rich antifungal proteins from ascomycetes provide a feasible basis for designing new antifungal molecules. The main structural elements responsible for folding, stability and antifungal activity are not fully understood, although this is an essential prerequisite for rational protein design. In this study, we used the Neosartorya fischeri antifungal protein (NFAP) to investigate the role of the disulphide bridges, the hydrophobic core, and the N-terminal amino acids in the formation of a highly stable, folded, and antifungal active protein...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28476772/biosynthesis-of-the-pharmaceutically-important-fungal-ergot-alkaloid-dihydrolysergic-acid-requires-a-specialized-allele-of-cloa
#6
Stephanie L Arnold, Daniel G Panaccione
Ergot alkaloids are specialized fungal metabolites that are important as the bases of several pharmaceuticals. Many ergot alkaloids are derivatives of lysergic acid (LA) and have vasoconstrictive activity, whereas several dihydrolysergic acid (DHLA) derivatives are vasorelaxant. The pathway to LA is established, with the P450 monooxygenase CloA playing a key role in oxidizing its substrate agroclavine to LA. We analyzed the activities of products of cloA alleles from different fungi relative to DHLA biosynthesis by expressing them in a mutant of the fungus Neosartorya fumigata that accumulates festuclavine, the precursor to DHLA...
July 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28039140/loop-3-of-fungal-endoglucanases-of-glycoside-hydrolase-family-12-modulates-catalytic-efficiency
#7
Hong Yang, Pengjun Shi, Yun Liu, Wei Xia, Xiaoyu Wang, Huifang Cao, Rui Ma, Huiying Luo, Yingguo Bai, Bin Yao
Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region affects the enzymatic properties of GH12 glucanases using NfEG12A from Neosartorya fischeri P1 and EG (PDB 1KS4) from Aspergillus niger Acidophilic and thermophilic NfEG12A had the highest catalytic efficiency (kcat/Km , 3,001 and 263 ml/mg/s toward lichenin and carboxymethyl cellulose sodium [CMC-Na], respectively) known so far...
March 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27988465/occurrence-and-ecological-distribution-of-heat-resistant-moulds-spores-hrms-in-raw-materials-used-by-food-industry-and-thermal-characterization-of-two-talaromyces-isolates
#8
Roberta Tranquillini, Nicoletta Scaramuzza, Elettra Berni
In this study, screening of some raw materials used to produce pasteurized products was carried out to determine the occurrence and ecological distribution of heat-resistant fungi. The search for Heat Resistant Mould Spores (HRMS) resulted in the isolation of a limited number of fungal genera: Arthrinium, Aspergillus with either Eurotium-type or Neosartorya-type ascoma, Byssochlamys, Hyphodermella, Monascus, Penicillium, Rasamsonia, Talaromyces and Thermoascus. Sexual aspergilli constituted an overwhelming percentage of the mycobiota, totaling 93...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27835989/a-penicillium-chrysogenum-based-expression-system-for-the-production-of-small-cysteine-rich-antifungal-proteins-for-structural-and-functional-analyses
#9
Christoph Sonderegger, László Galgóczy, Sandra Garrigues, Ádám Fizil, Attila Borics, Paloma Manzanares, Nikoletta Hegedüs, Anna Huber, Jose F Marcos, Gyula Batta, Florentine Marx
BACKGROUND: Small, cysteine-rich and cationic antifungal proteins (APs) from filamentous ascomycetes, such as NFAP from Neosartorya fischeri and PAF from Penicillium chrysogenum, are promising candidates for novel drug development. A prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. Technologies for structure analyses, such as electronic circular dichroism (ECD) or NMR spectroscopy, require highly purified samples and in case of NMR milligrams of uniformly (15)N-/(13)C-isotope labelled protein...
November 11, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27680770/chevalone-c-analogues-and-globoscinic-acid-derivatives-from-the-fungus-neosartorya-spinosa-kku-1nk1
#10
Oue-Artorn Rajachan, Kwanjai Kanokmedhakul, Wareerat Sanmanoch, Sophon Boonlue, Supa Hannongbua, Patchreenart Saparpakorn, Somdej Kanokmedhakul
Four meroterpenoids, 1-hydroxychevalone C, 1-acetoxychevalone C, 1,11-dihydroxychevalone C, and 11-hydroxychevalone C and two ester epimers, 2S,4S-spinosate and 2S,4R-spinosate, together with seven known compounds, chevalones B, C, and E, tryptoquivaline, nortryptoquivaline, tryptoquivaline L, and quinadoline A were isolated from the fungus Neosartorya spinosa. Their structures were established based on spectroscopic data analyses. The theoretical ECD spectra of epimers, 2S,4S-spinosate and 2S,4R-spinosate were calculated to support the experimental results of their CD spectra...
December 2016: Phytochemistry
https://www.readbyqxmd.com/read/27667521/development-of-a-dna-array-for-the-simple-identification-of-major-filamentous-fungi-in-the-beverage-manufacturing-environment
#11
Fuyuki Aoyama, Takahisa Miyamoto
Filamentous fungi were isolated from the indoor environment of a soft drink manufacturing plant and ordinary residences. The isolated strains were identified based on morphological observation and the nucleotide sequences of the region near the D2 region of the 26S rDNA. Three genera (Aspergillus, Penicillium, and Cladosporium) accounted for 48.1% of the fungal strains detected in the manufacturing plant and 75.3% in residences. A DNA array for identification of 15 genera and 26 species of filamentous fungi that were most frequently isolated from the manufacturing plant was developed...
2016: Biocontrol Science
https://www.readbyqxmd.com/read/27637945/nfap2-a-novel-cysteine-rich-anti-yeast-protein-from-neosartorya-fischeri-nrrl-181-isolation-and-characterization
#12
Liliána Tóth, Zoltán Kele, Attila Borics, László G Nagy, Györgyi Váradi, Máté Virágh, Miklós Takó, Csaba Vágvölgyi, László Galgóczy
The increasing incidence of fungal infections and damages due to drug-resistant fungi urges the development of new antifungal strategies. The cysteine-rich antifungal proteins from filamentous ascomycetes provide a feasible base for protection against molds due to their potent antifungal activity on them. In contrast to this, they show no or weak activity on yeasts, hence their applicability against this group of fungi is questionable. In the present study a 5.6 kDa anti-yeast protein (NFAP2) is isolated, identified and characterized from the ferment broth of Neosartorya fischeri NRRL 181...
December 2016: AMB Express
https://www.readbyqxmd.com/read/27530464/cytotoxic-activity-of-secondary-metabolites-from-marine-derived-fungus-neosartorya-siamensis-in-human-cancer-cells
#13
M Prata-Sena, A A Ramos, S Buttachon, B Castro-Carvalho, P Marques, T Dethoup, A Kijjoa, E Rocha
Compounds isolated from the marine sea fan-derived fungus Neosartorya siamensis (KUFA 0017), namely, 2,4-dihydroxy-3-methylacetophenon (1), chevalone C (2), nortryptoquivaline (4), tryptoquivaline H (6), tryptoquivaline F (7), fiscalin A (8), epi-fiscalin A (9), epi-neofiscalin A (11) and epi-fiscalin C (13) were tested for anti-proliferative activity by MTT assay, DNA damage induction by comet assay, and induction of cell death by nuclear condensation assay on colon HCT116, liver HepG2 and melanoma A375 cancer cell lines...
November 2016: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/27503553/heterologous-production-of-an-acidic-thermostable-lipase-with-broad-range-ph-activity-from-thermophilic-fungus-neosartorya-fischeri-p1
#14
Qiaoqiao Sun, Hui Wang, Huitu Zhang, Huiying Luo, Pengjun Shi, Yingguo Bai, Fuping Lu, Bin Yao, Huoqing Huang
Thermophilic Neosartorya fischeri P1 is an excellent lipase producer and harbors seven lipase genes. All genes were found to be functional after heterologous expression in Escherichia coli. One of them, LIP09, showed high-level expression in Pichia pastoris with the yield of 2.0 g/L in a 3.7-L fermentor. Deduced amino acid sequence of LIP09 consists of a putative signal peptide (residues 1-19) and a mature polypeptide (residues 20-562). Compared with other fungal counterparts, purified recombinant LIP09 has some superior properties...
November 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27498502/isolation-and-identification-of-gold-nanoparticles-synthesizing-fungi-from-indian-kolar-gold-field-mine-soil
#15
V Jhansi Lakshmi, K P Kannan
An indigenous fungal strain was isolated from Indian Kolar Gold Field mine soil. The isolate was heterothallic, branched septate, deeply floccose, fast-growing, dull green with white background conidial columnar mycelium from Aspergillus section Fumigati. Diverse metabolic patterns of the isolate exhibit high metal, thermal resistance which grews well from 28 ± 1 degrees C to 37 degrees C and pH concentration was significant on the growth of isolate. Phylogenetic analysis of 16srRNA β-Tubulin gene sequence established relationship among isolate and other taxa...
July 2016: Journal of Environmental Biology
https://www.readbyqxmd.com/read/27447650/new-cyclotetrapeptides-and-a-new-diketopiperzine-derivative-from-the-marine-sponge-associated-fungus-neosartorya-glabra-kufa-0702
#16
War War May Zin, Suradet Buttachon, Tida Dethoup, Carla Fernandes, Sara Cravo, Madalena M M Pinto, Luís Gales, José A Pereira, Artur M S Silva, Nazim Sekeroglu, Anake Kijjoa
Two new cyclotetrapeptides, sartoryglabramides A (5) and B (6), and a new analog of fellutanine A (8) were isolated, together with six known compounds including ergosta-4, 6, 8 (14), 22-tetraen-3-one, ergosterol 5, 8-endoperoxide, helvolic acid, aszonalenin (1), (3R)-3-(1H-indol-3-ylmethyl)-3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione (2), takakiamide (3), (11aR)-2,3-dihydro-1H-pyrrolo[2,1-c][1,4]benzodiazepine-5,11(10H,11aH)-dione (4), and fellutanine A (7), from the ethyl acetate extract of the culture of the marine sponge-associated fungus Neosartorya glabra KUFA 0702...
July 20, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27438842/new-polyketides-and-new-benzoic-acid-derivatives-from-the-marine-sponge-associated-fungus-neosartorya-quadricincta-kufa-0081
#17
Chadaporn Prompanya, Tida Dethoup, Luís Gales, Michael Lee, José A C Pereira, Artur M S Silva, Madalena M M Pinto, Anake Kijjoa
Two new pentaketides, including a new benzofuran-1-one derivative (1) and a new isochromen-1-one (5), and seven new benzoic acid derivatives, including two new benzopyran derivatives (2a, b), a new benzoxepine derivative (3), two new chromen-4-one derivatives (4b, 7) and two new benzofuran derivatives (6a, b), were isolated, together with the previously reported 2,3-dihydro-6-hydroxy-2,2-dimethyl-4H-1-benzopyran-4-one (4a), from the culture of the marine sponge-associated fungus Neosartorya quadricincta KUFA 0081...
July 16, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27400964/revisiting-overexpression-of-a-heterologous-%C3%AE-glucosidase-in-trichoderma-reesei-fusion-expression-of-the-neosartorya-fischeri-bgl3a-to-cbh1-enhances-the-overall-as-well-as-individual-cellulase-activities
#18
Xianli Xue, Yilan Wu, Xing Qin, Rui Ma, Huiying Luo, Xiaoyun Su, Bin Yao
BACKGROUND: The filamentous fungus Trichoderma reesei has the capacity to secret large amounts of cellulase and is widely used in a variety of industries. However, the T. reesei cellulase is weak in β-glucosidase activity, which results in accumulation of cellobiose inhibiting the endo- and exo-cellulases. By expressing an exogenous β-glucosidase gene, the recombinant T. reesei cellulase is expected to degrade cellulose into glucose more efficiently. RESULTS: The thermophilic β-glucosidase NfBgl3A from Neosartorya fischeri is chosen for overexpression in T...
July 11, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27268269/neofiscalin-a-and-fiscalin-c-are-potential-novel-indole-alkaloid-alternatives-for-the-treatment-of-multidrug-resistant-gram-positive-bacterial-infections
#19
Lucinda J Bessa, Suradet Buttachon, Tida Dethoup, Rosário Martins, Vitor Vasconcelos, Anake Kijjoa, Paulo Martins da Costa
Ten indole alkaloids were obtained from the marine sponge-associated fungus Neosartorya siamensis KUFA 0017. We studied the antimicrobial properties of these and of three other compounds previously isolated from the soil fungus N. siamensis KUFC 6349. Only neofiscalin A showed antimicrobial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecalis (VRE); with a minimum inhibitory concentration (MIC) of 8 μg mL(-1) against both strains...
August 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27242710/aspergillus-fumigatus-related-species-in-clinical-practice
#20
REVIEW
Frédéric Lamoth
Aspergillus fumigatus is the main etiologic agent of invasive aspergillosis (IA). Other Aspergillus species belonging to the section Fumigati (A. fumigatus complex) may occasionally be the cause of IA. These strains are often misidentified, as they cannot be distinguished from A. fumigatus by conventional morphological analysis and sequencing methods. This lack of recognition may have important consequences as these A. fumigatus-related species often display some level of intrinsic resistance to azoles and other antifungal drugs...
2016: Frontiers in Microbiology
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