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https://www.readbyqxmd.com/read/29689318/biotransformation-of-ruscogenins-by-cunninghamella-blakesleeana-nrrl-1369-and-neoruscogenin-by-endophytic-fungus-neosartorya-hiratsukae
#1
Özge Özçinar, Özgür Tağ, Hasan Yusufoglu, Bijen Kivçak, Erdal Bedir
Biotransformation of steroidal ruscogenins (neoruscogenin and ruscogenin) was carried out with Cunninghamella blakesleeana NRRL 1369 and endophytic fungus Neosartorya hiratsukae yielding mainly P450 monooxygenase products together with a glycosylated compound. Fermentation of ruscogenins (75:25, neoruscogenin-ruscogenin mixture) with C. blakesleeana yielded 8 previously undescribed hydroxylated compounds. Furthermore, microbial transformation of neoruscogenin by endophytic fungus N. hiratsukae afforded three previously undescribed neoruscogenin derivatives...
April 21, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29563903/anti-candidal-activity-and-functional-mapping-of-recombinant-and-synthetic-neosartorya-fischeri-antifungal-protein-2-nfap2
#2
Liliána Tóth, Györgyi Váradi, Attila Borics, Gyula Batta, Zoltán Kele, Ákos Vendrinszky, Roberta Tóth, Hargita Ficze, Gábor K Tóth, Csaba Vágvölgyi, Florentine Marx, László Galgóczy
The increasing number of life-threatening Candida infections caused by antifungal drug-resistant strains urges the development of new therapeutic strategies. The small, cysteine-rich, and cationic Neosartorya fischeri antifungal protein 2 (NFAP2) effectively inhibits the growth of Candida spp. Limiting factors of its future application, are the low-yield production by the native producer, unavailable information about potential clinical application, and the unsolved relationship between the structure and function...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29401749/the-fungal-metabolite-eurochevalierine-a-sequiterpene-alkaloid-displays-anti-cancer-properties-through-selective-sirtuin-1-2-inhibition
#3
Michael Schnekenburger, Véronique Mathieu, Florence Lefranc, Jun Young Jang, Marco Masi, Anake Kijjoa, Antonio Evidente, Hyun-Jung Kim, Robert Kiss, Mario Dicato, Byung Woo Han, Marc Diederich
NAD⁺-dependent histone deacetylases (sirtuins) are implicated in cellular processes such as proliferation, DNA repair, and apoptosis by regulating gene expression and the functions of numerous proteins. Due to their key role in cells, the discovery of small molecule sirtuin modulators has been of significant interest for diverse therapeutic applications. In particular, it has been shown that inhibition of sirtuin 1 and 2 activities is beneficial for cancer treatment. Here, we demonstrate that the fungal metabolite eurochevalierine from the fungus Neosartorya pseudofischeri inhibits sirtuin 1 and 2 activities (IC50 about 10 µM) without affecting sirtuin 3 activity...
February 5, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29333049/marine-derived-fungi-extracts-enhance-the-cytotoxic-activity-of-doxorubicin-in-nonsmall-cell-lung-cancer-cells-a459
#4
Bruno Castro-Carvalho, Alice A Ramos, Maria Prata-Sena, Fernanda Malhão, Márcia Moreira, Daniela Gargiulo, Tida Dethoup, Suradet Buttachon, Anake Kijjoa, Eduardo Rocha
Background: Drug resistance is a major concern in the current chemotherapeutic approaches and the combination with natural compounds may enhance the cytotoxic effects of the anticancer drugs. Therefore, this study evaluated the cytotoxicity of crude ethyl extracts of six marine-derived fungi - Neosartorya tsunodae KUFC 9213 (E1), Neosartorya laciniosa KUFC 7896 (E2), Neosartorya fischeri KUFC 6344 (E3), Aspergillus similanensis KUFA 0013 (E4), Neosartorya paulistensis KUFC 7894 (E5), and Talaromyces trachyspermum KUFC 0021 (E6) - when combined with doxorubicin (Dox), in seven human cancer cell lines...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29194412/a-new-dihydrochromone-dimer-and-other-secondary-metabolites-from-cultures-of-the-marine-sponge-associated-fungi-neosartorya-fennelliae-kufa-0811-and-neosartorya-tsunodae-kufc-9213
#5
Decha Kumla, Tin Shine Aung, Suradet Buttachon, Tida Dethoup, Luís Gales, José A Pereira, Ângela Inácio, Paulo M Costa, Michael Lee, Nazim Sekeroglu, Artur M S Silva, Madalena M M Pinto, Anake Kijjoa
A previously unreported dihydrochromone dimer, paecilin E ( 1 ), was isolated, together with eleven known compounds: β-sitostenone, ergosta-4,6,8 (14), 22-tetraen-3-one, cyathisterone, byssochlamic acid, dehydromevalonic acid lactone, chevalone B, aszonalenin, dankasterone A ( 2 ), helvolic acid, secalonic acid A and fellutanine A, from the culture filtrate extract of the marine sponge-associated fungus Neosartorya fennelliae KUFA 0811. Nine previously reported metabolites, including a chromanol derivative ( 3 ), (3β, 5α, 22 E ), 3,5-dihydroxyergosta-7,22-dien-6-one ( 4 ), byssochlamic acid, hopan-3β,22-diol, chevalone C, sartorypyrone B, helvolic acid, lumichrome and the alkaloid harmane were isolated from the culture of the marine-sponge associated fungus Neosartorya tsunodae KUFC 9213...
December 1, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29186777/clavine-alkaloids-gene-clusters-of-penicillium-and-related-fungi-evolutionary-combination-of-prenyltransferases-monooxygenases-and-dioxygenases
#6
REVIEW
Juan F Martin, Rubén Álvarez-Álvarez, Paloma Liras
The clavine alkaloids produced by the fungi of the Aspergillaceae and Arthrodermatacea families differ from the ergot alkaloids produced by Claviceps and Neotyphodium . The clavine alkaloids lack the extensive peptide chain modifications that occur in lysergic acid derived ergot alkaloids. Both clavine and ergot alkaloids arise from the condensation of tryptophan and dimethylallylpyrophosphate by the action of the dimethylallyltryptophan synthase. The first five steps of the biosynthetic pathway that convert tryptophan and dimethylallyl-pyrophosphate (DMA-PP) in chanoclavine-1-aldehyde are common to both clavine and ergot alkaloids...
November 24, 2017: Genes
https://www.readbyqxmd.com/read/29172518/ergot-alkaloid-biosynthesis-in-the-maize-zea-mays-ergot-fungus-claviceps-gigantea
#7
Paige E Bragg, Matthew D Maust, Daniel G Panaccione
Biosynthesis of the dihydrogenated forms of ergot alkaloids is of interest because many of the ergot alkaloids used as pharmaceuticals may be derived from dihydrolysergic acid (DHLA) or its precursor dihydrolysergol. The maize (Zea mays) ergot pathogen Claviceps gigantea has been reported to produce dihydrolysergol, a hydroxylated derivative of the common ergot alkaloid festuclavine. We hypothesized expression of C. gigantea cloA in a festuclavine-accumulating mutant of the fungus Neosartorya fumigata would yield dihydrolysergol because the P450 monooxygenase CloA from other fungi performs similar oxidation reactions...
December 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29024903/bacteria-mould-and-yeast-spore-inactivation-studies-by-scanning-electron-microscope-observations
#8
Siti N M Rozali, Elham A Milani, Rebecca C Deed, Filipa V M Silva
Spores are the most resistant form of microbial cells, thus difficult to inactivate. The pathogenic or food spoilage effects of certain spore-forming microorganisms have been the primary basis of sterilization and pasteurization processes. Thermal sterilization is the most common method to inactivate spores present on medical equipment and foods. High pressure processing (HPP) is an emerging and commercial non-thermal food pasteurization technique. Although previous studies demonstrated the effectiveness of thermal and non-thermal spore inactivation, the in-depth mechanisms of spore inactivation are as yet unclear...
December 18, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28827626/ergot-alkaloids-contribute-to-virulence-in-an-insect-model-of-invasive-aspergillosis
#9
Daniel G Panaccione, Stephanie L Arnold
Neosartorya fumigata (Aspergillus fumigatus) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a branch of the ergot alkaloid pathway called fumigaclavines. The biosynthesis of the N. fumigata ergot alkaloids and their relation to those produced by alternate pathway branches in fungi from the plant-inhabiting Clavicipitaceae have been well-characterised, but the potential role of these alkaloids in animal pathogenesis has not been studied extensively...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28772258/aspergilli-with-neosartorya-type-ascospores-heat-resistance-and-effect-of-sugar-concentration-on-growth-and-spoilage-incidence-in-berry-products
#10
Elettra Berni, Roberta Tranquillini, Nicoletta Scaramuzza, Andrea Brutti, Valentina Bernini
This study focused on four different heat resistant aspergilli: two strains of Aspergillus hiratsukae (≡Neosartorya hiratsukae), one strain of Aspergillus neoglaber (≡Neosartorya glabra), and one strain of Aspergillus thermomutatus (≡Neosartorya pseudofischeri), all isolated from spoiled pasteurized products. Their heat-resistance, the sugar concentration limiting their germination and growth in berry-based media, and a possible relation between the contamination levels of the raw materials used and the spoilage incidence in strawberry jams were assessed...
October 3, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28710744/cloning-and-characterization-of-f3pyc-gene-encoding-pyruvate-carboxylase-in-aspergillus-flavus-strain-f3
#11
Sadia Qayyum, Ibrar Khan, Zulfiqar Ahmad Bhatti, Changsheng Peng
Pyruvate carboxylase is a major enzyme for biosynthesis of organic acids like; citric acid, fumeric acid, and L-malic acid. These organic acids play very important role for biological remediation of heavy metals. In this study, gene walking method was used to clone and characterize pyruvate carboxylase gene (F3PYC) from heavy metal resistant indigenous fungal isolate Aspergillus flavus (F3). 3579 bp of an open reading frame which encodes 1193 amino acid protein (isoelectric point: 6.10) with a calculated molecular weight of 131...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28620689/silencing-of-a-second-dimethylallyltryptophan-synthase-of-penicillium-roqueforti-reveals-a-novel-clavine-alkaloid-gene-cluster
#12
Ángeles Fernández-Bodega, Rubén Álvarez-Álvarez, Paloma Liras, Juan F Martín
Penicillium roqueforti produces several prenylated indole alkaloids, including roquefortine C and clavine alkaloids. The first step in the biosynthesis of roquefortine C is the prenylation of tryptophan-derived dipeptides by a dimethylallyltryptophan synthase, specific for roquefortine biosynthesis (roquefortine prenyltransferase). A second dimethylallyltryptophan synthase, DmaW2, different from the roquefortine prenyltransferase, has been studied in this article. Silencing the gene encoding this second dimethylallyltryptophan synthase, dmaW2, proved that inactivation of this gene does not prevent the production of roquefortine C, but suppresses the formation of other indole alkaloids...
August 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28512317/structural-determinants-of-neosartorya-fischeri-antifungal-protein-nfap-for-folding-stability-and-antifungal-activity
#13
László Galgóczy, Attila Borics, Máté Virágh, Hargita Ficze, Györgyi Váradi, Zoltán Kele, Florentine Marx
The recent global challenges to prevent and treat fungal infections strongly demand for the development of new antifungal strategies. The structurally very similar cysteine-rich antifungal proteins from ascomycetes provide a feasible basis for designing new antifungal molecules. The main structural elements responsible for folding, stability and antifungal activity are not fully understood, although this is an essential prerequisite for rational protein design. In this study, we used the Neosartorya fischeri antifungal protein (NFAP) to investigate the role of the disulphide bridges, the hydrophobic core, and the N-terminal amino acids in the formation of a highly stable, folded, and antifungal active protein...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28476772/biosynthesis-of-the-pharmaceutically-important-fungal-ergot-alkaloid-dihydrolysergic-acid-requires-a-specialized-allele-of-cloa
#14
Stephanie L Arnold, Daniel G Panaccione
Ergot alkaloids are specialized fungal metabolites that are important as the bases of several pharmaceuticals. Many ergot alkaloids are derivatives of lysergic acid (LA) and have vasoconstrictive activity, whereas several dihydrolysergic acid (DHLA) derivatives are vasorelaxant. The pathway to LA is established, with the P450 monooxygenase CloA playing a key role in oxidizing its substrate agroclavine to LA. We analyzed the activities of products of cloA alleles from different fungi relative to DHLA biosynthesis by expressing them in a mutant of the fungus Neosartorya fumigata that accumulates festuclavine, the precursor to DHLA...
July 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28039140/loop-3-of-fungal-endoglucanases-of-glycoside-hydrolase-family-12-modulates-catalytic-efficiency
#15
Hong Yang, Pengjun Shi, Yun Liu, Wei Xia, Xiaoyu Wang, Huifang Cao, Rui Ma, Huiying Luo, Yingguo Bai, Bin Yao
Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region affects the enzymatic properties of GH12 glucanases using NfEG12A from Neosartorya fischeri P1 and EG (PDB 1KS4) from Aspergillus niger Acidophilic and thermophilic NfEG12A had the highest catalytic efficiency (kcat/Km , 3,001 and 263 ml/mg/s toward lichenin and carboxymethyl cellulose sodium [CMC-Na], respectively) known so far...
March 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27988465/occurrence-and-ecological-distribution-of-heat-resistant-moulds-spores-hrms-in-raw-materials-used-by-food-industry-and-thermal-characterization-of-two-talaromyces-isolates
#16
Roberta Tranquillini, Nicoletta Scaramuzza, Elettra Berni
In this study, screening of some raw materials used to produce pasteurized products was carried out to determine the occurrence and ecological distribution of heat-resistant fungi. The search for Heat Resistant Mould Spores (HRMS) resulted in the isolation of a limited number of fungal genera: Arthrinium, Aspergillus with either Eurotium-type or Neosartorya-type ascoma, Byssochlamys, Hyphodermella, Monascus, Penicillium, Rasamsonia, Talaromyces and Thermoascus. Sexual aspergilli constituted an overwhelming percentage of the mycobiota, totaling 93...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27835989/a-penicillium-chrysogenum-based-expression-system-for-the-production-of-small-cysteine-rich-antifungal-proteins-for-structural-and-functional-analyses
#17
Christoph Sonderegger, László Galgóczy, Sandra Garrigues, Ádám Fizil, Attila Borics, Paloma Manzanares, Nikoletta Hegedüs, Anna Huber, Jose F Marcos, Gyula Batta, Florentine Marx
BACKGROUND: Small, cysteine-rich and cationic antifungal proteins (APs) from filamentous ascomycetes, such as NFAP from Neosartorya fischeri and PAF from Penicillium chrysogenum, are promising candidates for novel drug development. A prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. Technologies for structure analyses, such as electronic circular dichroism (ECD) or NMR spectroscopy, require highly purified samples and in case of NMR milligrams of uniformly (15)N-/(13)C-isotope labelled protein...
November 11, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27680770/chevalone-c-analogues-and-globoscinic-acid-derivatives-from-the-fungus-neosartorya-spinosa-kku-1nk1
#18
Oue-Artorn Rajachan, Kwanjai Kanokmedhakul, Wareerat Sanmanoch, Sophon Boonlue, Supa Hannongbua, Patchreenart Saparpakorn, Somdej Kanokmedhakul
Four meroterpenoids, 1-hydroxychevalone C, 1-acetoxychevalone C, 1,11-dihydroxychevalone C, and 11-hydroxychevalone C and two ester epimers, 2S,4S-spinosate and 2S,4R-spinosate, together with seven known compounds, chevalones B, C, and E, tryptoquivaline, nortryptoquivaline, tryptoquivaline L, and quinadoline A were isolated from the fungus Neosartorya spinosa. Their structures were established based on spectroscopic data analyses. The theoretical ECD spectra of epimers, 2S,4S-spinosate and 2S,4R-spinosate were calculated to support the experimental results of their CD spectra...
December 2016: Phytochemistry
https://www.readbyqxmd.com/read/27667521/development-of-a-dna-array-for-the-simple-identification-of-major-filamentous-fungi-in-the-beverage-manufacturing-environment
#19
Fuyuki Aoyama, Takahisa Miyamoto
Filamentous fungi were isolated from the indoor environment of a soft drink manufacturing plant and ordinary residences. The isolated strains were identified based on morphological observation and the nucleotide sequences of the region near the D2 region of the 26S rDNA. Three genera (Aspergillus, Penicillium, and Cladosporium) accounted for 48.1% of the fungal strains detected in the manufacturing plant and 75.3% in residences. A DNA array for identification of 15 genera and 26 species of filamentous fungi that were most frequently isolated from the manufacturing plant was developed...
2016: Biocontrol Science
https://www.readbyqxmd.com/read/27637945/nfap2-a-novel-cysteine-rich-anti-yeast-protein-from-neosartorya-fischeri-nrrl-181-isolation-and-characterization
#20
Liliána Tóth, Zoltán Kele, Attila Borics, László G Nagy, Györgyi Váradi, Máté Virágh, Miklós Takó, Csaba Vágvölgyi, László Galgóczy
The increasing incidence of fungal infections and damages due to drug-resistant fungi urges the development of new antifungal strategies. The cysteine-rich antifungal proteins from filamentous ascomycetes provide a feasible base for protection against molds due to their potent antifungal activity on them. In contrast to this, they show no or weak activity on yeasts, hence their applicability against this group of fungi is questionable. In the present study a 5.6 kDa anti-yeast protein (NFAP2) is isolated, identified and characterized from the ferment broth of Neosartorya fischeri NRRL 181...
December 2016: AMB Express
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