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https://www.readbyqxmd.com/read/29244856/in-vitro-anthelmintic-effects-of-spigelia-anthelmia-protein-fractions-against-haemonchus-contortus
#1
Sandra Alves Araújo, Alexandra Martins Dos Santos Soares, Carolina Rocha Silva, Eduardo Bezerra Almeida Júnior, Cláudia Quintino Rocha, André Teixeira da Silva Ferreira, Jonas Perales, Livio M Costa-Júnior
Gastrointestinal nematodes are a significant concern for animal health and well-being, and anthelmintic treatment is mainly performed through the use of chemical products. However, bioactive compounds produced by plants have shown promise for development as novel anthelmintics. The aim of this study is to assess the anthelmintic activity of protein fractions from Spigelia anthelmia on the gastrointestinal nematode Haemonchus contortus. Plant parts were separated into leaves, stems and roots, washed with distilled water, freeze-dried and ground into a fine powder...
2017: PloS One
https://www.readbyqxmd.com/read/29244059/the-mediterranean-diet-metabolic-and-molecular-mechanisms
#2
Valeria Tosti, Beatrice Bertozzi, Luigi Fontana
Consuming a Mediterranean diet rich in minimally processed plant foods has been associated with a reduced risk of developing multiple chronic diseases and increased life expectancy. Data from several randomized clinic trials have demonstrated a beneficial effect in the primary and secondary prevention of cardiovascular disease, type 2 diabetes, atrial fibrillation and breast cancer. The exact mechanism by which an increased adherence to the traditional Mediterranean diet exerts its favorable effects is not known...
December 13, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29243425/-overexpression-and-isolation-of-cyp76ah1-in-salvia-miltiorrhiza-hairy-roots
#3
Meng-Die Qi, Jian Wang, Xiao-Jing Ma, Quan Zhang, Fang-Fang Wang, Ying Kang, Yong Liu, Hui-Xin Lin
Protein complexes are involved in the synthesis of multiple secondary metabolites in plants, and their separation is essential to elucidate plant secondary metabolism and improve in vitro catalytic efficiency. In this study, the transgenic hairy roots of CYP76AH1, a key enzyme of tanshinone synthesis pathway, was constructed and the transgenic hairy roots of Danshen overexpressing CYP76AH1 protein were screened by Western blotting and used as a tissue culture material for the subsequent extraction of protein complex in tanshinone synthesis pathway...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29243177/24-epibrassinolide-induced-alterations-in-the-root-cell-walls-of-cucumis-sativus-l-under-ca-no3-2-stress
#4
Ya-Hong An, Heng Zhou, Ying-Hui Yuan, Lin Li, Jin Sun, Sheng Shu, Shi-Rong Guo
Brassinosteroids (BRs) can effectively alleviate the oxidative stress caused by Ca(NO3)2 in cucumber seedlings. The root system is an essential organ in plants due to its roles in physical anchorage, water and nutrient uptake, and metabolite synthesis and storage. In this study, 24-epibrassinolide (EBL) was applied to the cucumber seedling roots under Ca(NO3)2 stress, and the resulting chemical and anatomical changes were characterized to investigate the roles of BRs in alleviating salinity stress. Ca(NO3)2 alone significantly induced changes in the components of cell wall, anatomical structure, and expression profiles of several lignin biosynthetic genes...
December 14, 2017: Protoplasma
https://www.readbyqxmd.com/read/29241147/combined-enzymatic-and-metabolic-analysis-of-grapevine-cell-responses-to-elicitors
#5
Yuko Krzyzaniak, Jonathan Negrel, Christelle Lemaitre-Guillier, Gilles Clément, Grégory Mouille, Agnès Klinguer, Sophie Trouvelot, Marie-Claire Héloir, Marielle Adrian
Elicitors trigger plant defense responses, including phytoalexin production and cell-wall reinforcement. Primary metabolism plays an important role in these responses as it fuels the associated energetic costs and provides precursors for the synthesis of the numerous secondary metabolites involved in defenses against pathogens. In this context, we aimed to determine whether oligosaccharidic elicitors differing in their capacity to activate defense-associated secondary metabolism in grapevine would differently impact primary metabolism...
December 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29240841/microcystin-lr-does-not-induce-alterations-to-transcriptomic-or-metabolomic-profiles-of-a-model-heterotrophic-bacterium
#6
Robbie M Martin, Stephen P Dearth, Gary R LeCleir, Shawn R Campagna, Elizabeth M Fozo, Erik R Zinser, Steven W Wilhelm
Microcystins are secondary metabolites produced by several freshwater, bloom-forming cyanobacterial species. Microcystin-producing cyanobacteria co-occur with a complex community of heterotrophic bacteria. Though conflicting, studies suggest that microcystins affect the physiology of heterotrophic bacteria by inducing oxidative stress and increasing cell envelope permeability. Based on these observations, we hypothesized that exposure to microcystin should induce differential expression in genes responding to oxidative and envelope stress and trigger shifts in metabolite pools...
2017: PloS One
https://www.readbyqxmd.com/read/29240271/chromatin-level-regulation-of-the-fragmented-dothistromin-gene-cluster-in-the-forest-pathogen-dothistroma-septosporum
#7
Pranav Chettri, Pierre-Yves Dupont, Rosie E Bradshaw
Genes required for fungal secondary metabolite production are usually clustered, co-regulated and expressed in stationary growth phase. Chromatin modification has an important role in co-regulation of secondary metabolite genes. The virulence factor dothistromin, a relative of aflatoxin, provided a unique opportunity to study chromatin level regulation in a highly fragmented gene cluster that is switched on during early exponential growth phase. We analysed three histone modification marks by ChIP-qPCR and gene deletion in the pine pathogen Dothistroma septosporum to determine their effects on dothistromin gene expression across a time course and at different loci of the dispersed gene cluster...
December 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29240233/blue-honeysuckle-lonicera-caerulea-subsp-edulis-turcz-ex-herder-hult%C3%A3-n-berries-and-changes-in-their-ingredients-across-different-locations
#8
Mateja Senica, Martina Bavec, Franci Stampar, Maja Mikulic-Petkovsek
BACKGROUND: Interest in organic blue honeysuckle berries has increased in recent years. They are rich in various health promoting compounds, which are sensitive to different environmental factors and are modified during the growing season. RESULTS: Honeysuckle berries from different locations significantly differed in contents of primary and secondary metabolites. The location Ogulin, with the highest altitude and, consequently, high UV-radiation, had the highest phenolics content (259...
December 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29239784/new-perspectives-for-natural-triterpene-glycosides-as-potential-adjuvants
#9
Marie-Aleth Lacaille-Dubois, Hildebert Wagner
BACKGROUND: Triterpene glycosides are a vast group of secondary metabolites widely distributed in plants including a high number of biologically active compounds. The pharmacological potential is evaluated by using many bioassays particularly in the field of cancerology, immunology, and microbiology. The adjuvant concept is well known for these molecules in vaccines, but there is little preclinical evidence to support this concept in the management of cancer, infections and inflammation...
November 7, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29239523/iridoides-of-chemotaxonomy-relevance-a-new-antirrhinoside-ester-and-other-constituents-from-kickxia-spuria%C3%A2-subsp-integrifolia-brot-r-fern
#10
Alessandro Venditti, Claudio Frezza, Ilaria Serafini, Alessandro Ciccòla, Fabio Sciubba, Mauro Serafini, Armandodoriano Bianco
In this work, the secondary metabolite content of the ethanolic extract of Kickxia spuria subsp. integrifolia (Brot.) R. Fern. is reported. Fourteen compounds were isolated and identified by means of Column Chromatography and NMR and MS spectrometric techniques, respectively. Among these compounds, the chemotaxonomic markers of the species were evidenced whereas others were reported for the first time in the genus. Among these a new antirrhinoside derivative (12) was recognized. The iridoid content show a molecular pattern very similar to those reported for other taxa comprised in the Antirrhinae tribe of Plantaginaceae, thus providing an additional evidence to support the current botanical classification of the Kickxia genus...
December 14, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29238329/relative-quantitative-proteomic-analysis-of-brucella-abortus-reveals-metabolic-adaptation-to-multiple-environmental-stresses
#11
Xiaodong Zai, Qiaoling Yang, Ying Yin, Ruihua Li, Mengying Qian, Taoran Zhao, Yaohui Li, Jun Zhang, Ling Fu, Junjie Xu, Wei Chen
Brucella spp. are facultative intracellular pathogens that cause chronic brucellosis in humans and animals. The virulence of Brucella primarily depends on its successful survival and replication in host cells. During invasion of the host tissue, Brucella is simultaneously subjected to a variety of harsh conditions, including nutrient limitation, low pH, antimicrobial defenses, and extreme levels of reactive oxygen species (ROS) via the host immune response. This suggests that Brucella may be able to regulate its metabolic adaptation in response to the distinct stresses encountered during its intracellular infection of the host...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29237703/genome-assembly-and-annotation-of-the-medicinal-plant-calotropis-gigantea-a-producer-of-anti-cancer-and-anti-malarial-cardenolides
#12
Genevieve M Hoopes, John P Hamilton, Jeongwoon Kim, Dongyan Zhao, Krystle Wiegert-Rininger, Emily Crisovan, C Robin Buell
Calotropis gigantea produces specialized secondary metabolites known as cardenolides which have anti-cancer and anti-malarial properties. Although transcriptomic studies have been conducted in other cardenolide-producing species, no nuclear genome assembly for an Asterid cardenolide-producing species has been reported to date. A high quality de novo assembly was generated for C. gigantea, representing 157,284,427 bp with an N50 scaffold size of 805,959 bp, for which quality assessments indicated a near complete representation of the genic space...
December 12, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29236928/leishmanicidal-compounds-of-nectria-pseudotrichia-an-endophytic-fungus-isolated-from-the-plant-caesalpinia-echinata-brazilwood
#13
Betania Barros Cota, Luiza Guimarães Tunes, Daniela Nabak Bueno Maia, Jonas Pereira Ramos, Djalma Menezes de Oliveira, Markus Kohlhoff, Tânia Maria de Almeida Alves, Elaine Maria Souza-Fagundes, Fernanda Fraga Campos, Carlos Leomar Zani
BACKGROUND In a screen of extracts from plants and fungi to detect antileishmanial activity, we found that the ethyl acetate extract of the fungus Nectria pseudotrichia, isolated from the tree Caesalpinia echinata (Brazilwood), is a promising source of bioactive compounds. OBJECTIVES The aims of this study were to isolate and determine the chemical structures of the compounds responsible for the antileishmanial activity of the organic extract from N. pseudotrichia. METHODS Compounds were isolated by chromatographic fractionation using semi-preparative high-performance liquid chromatography, and their chemical structures were determined by analytical and spectral data and by comparison with published data...
February 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29236677/a-chemometrics-approach-for-nuclear-magnetic-resonance-data-to-characterize-the-partial-metabolome-banana-peels-from-southern-brazil
#14
Sara Cardoso, Marcelo Maraschin, Luiz Augusto Martins Peruch, Miguel Rocha, Aline Pereira
Banana peels are well recognized as a source of important bioactive compounds, such as phenolics, carotenoids, biogenic amines, among others. As such, they have recently started to be used for industrial purposes. However, its composition seems to be strongly affected by biotic or abiotic ecological factors. Thus, this study aimed to investigate banana peels chemical composition, not only to get insights on eventual metabolic changes caused by the seasons, in southern Brazil, but also to identify the most relevant metabolites for these processes...
December 13, 2017: Journal of Integrative Bioinformatics
https://www.readbyqxmd.com/read/29234729/a-comprehensive-study-of-eco-friendly-natural-pigment-and-its-applications
#15
Ramendra Singh Parmar, Charu Singh
Actinomycetes, a large group of filamentous bacteria account for 70-80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography...
March 2018: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29234160/inheritance-patterns-in-metabolism-and-growth-in-diallel-crosses-of-arabidopsis-thaliana-from-a-single-growth-habitat
#16
Andrés Eduardo Rodriguez Cubillos, Hao Tong, Saleh Alseekh, Francisco de Abreu E Lima, Jing Yu, Alisdair R Fernie, Zoran Nikoloski, Roosa A E Laitinen
Metabolism is a key determinant of plant growth and modulates plant adaptive responses. Increased metabolic variation due to heterozygosity may be beneficial for highly homozygous plants if their progeny is to respond to sudden changes in the habitat. Here, we investigate the extent to which heterozygosity contributes to the variation in metabolism and size of hybrids of Arabidopsis thaliana whose parents are from a single growth habitat. We created full diallel crosses among seven parents, originating from Southern Germany, and analysed the inheritance patterns in primary and secondary metabolism as well as in rosette size in situ...
December 13, 2017: Heredity
https://www.readbyqxmd.com/read/29232862/effect-of-oxylipins-terpenoid-precursors-and-wounding-on-soft-corals-secondary-metabolism-as-analyzed-via-uplc-ms-and-chemometrics
#17
Mohamed A Farag, Hildegard Westphal, Tarek F Eissa, Ludger A Wessjohann, Achim Meyer
The effect of three oxylipin analogues, a terpenoid intermediate and wounding on the secondary metabolism of the soft corals Sarcophyton glaucum and Lobophyton pauciflorum was assessed. Examined oxylipins included prostaglandin (PG-E1), methyl jasmonate (MeJA), and arachidonic acid (AA) in addition to the diterpene precursor geranylgeranylpyrophosphate (GGP). Post-elicitation, metabolites were extracted from coral heads and analyzed via UPLC-MS followed by multivariate data analyses. Both supervised and unsupervised data analyses were used for sample classification...
December 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29232373/disentangling-metabolic-functions-of-bacteria-in-the-honey-bee-gut
#18
Lucie Kešnerová, Ruben A T Mars, Kirsten M Ellegaard, Michaël Troilo, Uwe Sauer, Philipp Engel
It is presently unclear how much individual community members contribute to the overall metabolic output of a gut microbiota. To address this question, we used the honey bee, which harbors a relatively simple and remarkably conserved gut microbiota with striking parallels to the mammalian system and importance for bee health. Using untargeted metabolomics, we profiled metabolic changes in gnotobiotic bees that were colonized with the complete microbiota reconstituted from cultured strains. We then determined the contribution of individual community members in mono-colonized bees and recapitulated our findings using in vitro cultures...
December 2017: PLoS Biology
https://www.readbyqxmd.com/read/29231777/de-novo-transcriptome-study-identifies-candidate-genes-involved-in-resistance-to-austropuccinia-psidii-myrtle-rust-in-syzygium-luehmannnii-riberry
#19
Peri Ann Tobias, David Guest, Carsten Külheim, Robert F Park
Austropuccinia psidii, causal agent of myrtle rust, was discovered in Australia in 2010 and has since become established on a wide range of species within the Myrtaceae. Syzygium luehmannii, endemic to Australia, is an increasingly valuable berry crop. Plants were screened for responses to A. psidii inoculation and specific resistance, in the form of localised necrosis, was determined in 29% of individuals. To understand the molecular basis underlying this response, mRNA was sequenced from leaf samples taken pre-inoculation, and at 24 and 48 hours post inoculation, from four resistant and four susceptible plants...
December 12, 2017: Phytopathology
https://www.readbyqxmd.com/read/29231702/stacked-injections-of-biphasic-extractions-for-improved-metabolomic-coverage-and-sample-throughput
#20
Corey David Broeckling, Jessica E Prenni
Omics technologies attempt to provide comprehensive coverage of their target analytes. Comprehensive coverage of metabolites - the aim of non-targeted metabolomics applications - is hindered by the extreme diversity in physiochemical properties of the metabolome. One approach to deal with this challenge is the use of biphasic extractions. These methods generate two largely complementary extracts from a single sample, with an organic lipid-rich fraction and an aqueous fraction containing largely primary and secondary metabolites...
December 12, 2017: Analytical Chemistry
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