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https://www.readbyqxmd.com/read/29328937/utilization-of-methanol-in-crude-glycerol-to-assist-lipid-production-in-non-sterilized-fermentation-from-trichosporon-oleaginosus
#1
Jiaxin Chen, Xiaolei Zhang, Rajeshwar Dayal Tyagi, Patrick Drogui
In this work, methanol in crude glycerol solution was used to assist the lipid production with oleaginous yeast Trichosporon oleaginosus cultivated under non-sterilized conditions. The investigated methanol concentration was 0%, 1.4%, 2.2%, 3.3% and 4.4% (w/v). The results showed that methanol played a significant role in the non-sterilized fermentation for lipid production. The optimal methanol concentration was around 1.4% (w/v) in which the growth of T. oleaginosus was promoted and overcame that of the contaminants...
January 3, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29327194/agro-industrial-waste-recycling-by-trichosporon-fermentans-conversion-of-waste-sweetpotato-vines-alone-into-lipid
#2
Qi Shen, Yue Chen, Hui Lin, Qun Wang, Yuhua Zhao
Agro-industrial waste can be used to replace traditional carbohydrates, such as sucrose, starch, and glucose in many industrial fermentation processes. This study investigated the conversion of pre-treated waste sweetpotato vines (SV) into lipid by Trichosporon fermentans under the separate hydrolysis and fermentation (SHF) and the simultaneous saccharification and fermentation (SSF) processes. The results showed that SV autoclaving significantly increased the lipid accumulation of T. fermentans compared with acid or alkaline hydrolysis...
January 12, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29306852/trichosporon-asahii-septic-thrombophlebitis-following-lower-extremity-amputation-in-an-immunocompetent-host
#3
Pradeep Kumar Mada, Folusakin Ayoade, Ailing Li, John Todd
A 59-year-old man with a history of peripheral vascular disease status post femoral popliteal bypass presented with critical limb ischaemia of the left leg. An arterial Doppler ultrasound showed an occluded graft requiring an above knee amputation. Five days after surgery, the patient developed fever, leucocytosis, significant stump swelling and pain, and serosanguinous discharge from his wound. Wound swab cultures from the stump grew Trichosporon asahii A venous Doppler ultrasound revealed extensive thrombosis of the left lower extremity...
January 6, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29296646/trichosporon-inkin-causing-invasive-infection-with-multiple-skin-abscesses-in-a-renal-transplant-patient-successfully-treated-with-voriconazole
#4
Arnaud Jannic, Matthieu Lafaurie, Blandine Denis, Samia Hamane, Fabien Metivier, Michel Rybojad, Jean-David Bouaziz, Martine Bagot, Marie Jachiet
No abstract text is available yet for this article.
January 2018: JAAD Case Reports
https://www.readbyqxmd.com/read/29288263/an-unusual-infection-in-a-child-with-congenital-heart-disease-trichosporon-asahii-infection-with-rapid-diagnosis-by-18s-ribonucleic-acid-rna
#5
Robert Grignani, Evelyn Sc Koay, Swee Chye Quek, Christian Harkensee
No abstract text is available yet for this article.
November 2017: Annals of the Academy of Medicine, Singapore
https://www.readbyqxmd.com/read/29287630/biofilm-formation-and-antifungal-susceptibility-of-trichosporon-asahii-isolates-from-mexican-patients
#6
Alexandra M Montoya, Mariana Elizondo-Zertuche, Rogelio de J Treviño-Rangel, Miguel Becerril-García, Gloria M González
BACKGROUND: Trichosporon asahii is a yeast-like fungus that has recently gained importance as a cause of opportunistic systemic infections. The pathogenicity and virulence factors of T. asahii remain largely unknown. Because of the association between invasive infections and the use of catheters and related devices, the ability of the microorganism to adhere and form biofilms may play an important role in the pathogenicity during a trichosporonosis. AIMS: The aim of this study is to identify an association between biofilm formation by T...
December 26, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/29261697/microbial-cells-can-cooperate-to-resist-high-level-chronic-ionizing-radiation
#7
Igor Shuryak, Vera Y Matrosova, Elena K Gaidamakova, Rok Tkavc, Olga Grichenko, Polina Klimenkova, Robert P Volpe, Michael J Daly
Understanding chronic ionizing radiation (CIR) effects is of utmost importance to protecting human health and the environment. Diverse bacteria and fungi inhabiting extremely radioactive waste and disaster sites (e.g. Hanford, Chernobyl, Fukushima) represent new targets of CIR research. We show that many microorganisms can grow under intense gamma-CIR dose rates of 13-126 Gy/h, with fungi identified as a particularly CIR-resistant group of eukaryotes: among 145 phylogenetically diverse strains tested, 78 grew under 36 Gy/h...
2017: PloS One
https://www.readbyqxmd.com/read/29259317/integrated-transcriptomic-analysis-of-trichosporon-asahii-uncovers-the-core-genes-and-pathways-of-fluconazole-resistance
#8
Haitao Li, Congmin Wang, Yong Chen, Shaoqiang Zhang, Rongya Yang
Trichosporon asahii (T. asahii) has emerged as a dangerous pathogen that causes rare but life-threatening infections. Its resistance to certain antifungal agents makes it difficult to treat, especially for patients undergoing long-term antibiotic therapy. In this study, we performed a series of fluconazole (FLC) perturbation experiments for two T. asahii strains, a clinical isolate stain CBS 2479 (T2) and an environmental isolate strain CBS 8904 (T8), to uncover potential genes and pathways involved in FLC resistance...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29249757/-familial-summer-type-hypersensitivity-pneumonitis-induced-in-summer-and-winter-at-the-work-place
#9
Toshihisa Ishikawa, Tsutomu Kawasaki, Kazuhiro Shimaya, Yukiko Abe, Yuki Kata, Kaori Okayasu, Koji Unoura, Yoichi Nakamura
We report two family members, a 64-year-old woman (patient 1) and her 37-year-old son (patient 2) diagnosed with summer-type hypersensitivity pneumonitis (SHP). Both patients had high serum titers of anti-Trichosporon asahii antibody. The patients lived in the same house and worked in the same barbershop. Patient 1 was diagnosed with SHP in the summer, and she reacted positively to the provocation test at the work place, but not in the house. Patient 2 was diagnosed with SHP in the winter. Generally, SHP develops and is diagnosed in the summer...
2017: Arerugī, [Allergy]
https://www.readbyqxmd.com/read/29241318/polyphenolic-content-antiradical-activity-stability-and-microbiological-quality-of-elderberry-sambucus-nigra-l-extracts
#10
Barbara Pliszka
BACKGROUND: The pharmaceutical and food industries expect detailed knowledge on the physicochemical properties of elderberry fruit extracts, their stability and microbiological quality, as well as the polyphenol content in elderberry cultivars. The characteristics of the extracts might be additionally modified by citric acid, which improves the stability of anthocyanins and protects processed fruits and syrups from pathogenic microorganisms. The choice of the method with citric acid was a consequence of the physicochemical charac teristics of elderberry pigments, which are not stable under the effect of light in alcoholic solutions...
October 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29196924/cutaneotrichosporon-trichosporon-debeurmannianum-a-rare-yeast-isolated-from-blood-and-urine-samples
#11
Reema Nath, Pallabi Sargiary, Biswajyoti Borkakoty, Pratap Parida
Cutaneotrichosporon (Trichosporon) debeurmannianum is a rarely isolated yeast from clinical samples. Nine isolates of this yeast were identified from clinical samples within a period of 3 years from June 2012 to May 2015. These isolates were from blood and urine samples sent to a clinical mycology laboratory of a tertiary care hospital in Assam, North East India. Clinically, the patients were diagnosed as septicemia and urinary tract infection. The age of the patients ranged from 2 to 50 years. Identification was made by sequencing the ITS region of ribosomal RNA gene...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29196923/mixed-infection-of-respiratory-tract-in-a-dog-caused-by-rhodotorula-mucilaginosa-and-trichosporon-jirovecii-a-case-report
#12
Małgorzata J Biegańska, Magdalena Rzewuska, Iwona Dąbrowska, Bożena Malewska-Biel, Magdalena Ostrzeszewicz, Bożena Dworecka-Kaszak
This report describes the isolation of two environmental fungi: Rhodotorula mucilaginosa and Trichosporon jirovecii accompanied by Pseudomonas aeruginosa and Escherichia coli from a dog with bronchotracheitis. All microorganisms were isolated routinely from a mucopurulent discharge, obtained during bronchoscopy from laryngotracheal area. The initial identification of yeasts was confirmed by API Candida and by molecular analysis of internal transcribed spacer region. Administered antimicrobial treatment with Marbofloxacin and Canizol has brought the improvement in the dogs' health status...
December 1, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29174563/invasive-fungi-derived-defensins-kill-drug-resistant-bacterial-pathogens
#13
Jiajia Wu, Shijie Liu, Hao Wang
Fungi-derived defensins are a class of antimicrobial peptides with therapeutic potential due to their high antibacterial efficacy and low toxicity. Based on the genomic strategy, we have identified 68 fungal defensin-like peptides (fDLPs) in five new genera, including Trichosporon, Apophysomyces, Lichtheimia, Beauveria and Scedosporium and characterized a new synthetic defensin (scedosporisin) from an invasive fungus. It was active against Gram-positive bacteria but not active against negative bacteria. Importantly, it killed several clinical resistant isolates such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci at low molecular concentrations...
November 21, 2017: Peptides
https://www.readbyqxmd.com/read/29167664/metabolic-engineering-of-oleaginous-yeasts-for-production-of-fuels-and-chemicals
#14
REVIEW
Shuobo Shi, Huimin Zhao
Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on Yarrowia that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as Rhodosporidium, Trichosporon, and Lipomyces...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29157237/isolation-and-identification-of-culturable-fungi-from-the-genitals-and-semen-of-healthy-giant-pandas-ailuropoda-melanoleuca
#15
Xiaoping Ma, Changcheng Li, Jiafa Hou, Yu Gu
BACKGROUND: In order to better understand the possible role of fungi in giant panda reproduction and overall health, it is important to provide a baseline for the normal fungal composition in the reproductive system. Using morphology and internal transcribed spacer (ITS) sequence analysis, we systematically isolated and identified fungal species from the vagina, foreskin, and semen of 21 (11 males and 10 females) healthy giant pandas to understand the normal fungal flora of the genital tracts...
November 21, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29152230/rare-fungal-infectious-agents-a-lurking-enemy
#16
REVIEW
Anna Skiada, Ioannis Pavleas, Maria Drogari-Apiranthitou
In the expanding population of immunocompromised patients and those treated in intensive care units, rare fungal infectious agents have emerged as important pathogens, causing invasive infections associated with high morbidity and mortality. These infections may present either as de novo or as breakthrough invasive infections in high-risk patients with hematologic malignancies receiving prophylactic or empirical antifungal therapy or in patients with central venous catheters. Diagnosis and treatment are challenging...
2017: F1000Research
https://www.readbyqxmd.com/read/29149902/metabolism-of-aromatics-by-trichosporon-oleaginosus-while-remaining-oleaginous
#17
Allison Yaguchi, Alana Robinson, Erin Mihealsick, Mark Blenner
BACKGROUND: The oleaginous yeast, Trichosporon oleaginosus, has been extensively studied for its ability to metabolize non-conventional feedstocks. These include phenol-containing waste streams, such as distillery wastewater, or streams consisting of non-conventional sugars, such as hydrolyzed biomass and various bagasse. An initial BLAST search suggests this yeast has putative aromatic metabolizing genes. Given the desirability to valorize underutilized feedstocks such as lignin, we investigated the ability of T...
November 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29129976/using-odd-alkanes-as-a-carbon-source-to-increase-the-content-of-nutritionally-important-fatty-acids-in-candida-krusei-trichosporon-cutaneum-and-yarrowia-lipolytica
#18
Olga Matatkova, Lucia Gharwalova, Michal Zimola, Tomas Rezanka, Jan Masak, Irena Kolouchova
We investigated the possibility of utilizing unusual carbon sources by three yeast strains: Candida krusei DBM 2136, Trichosporon cutaneum CCY 30-5-10, and Yarrowia lipolytica CCY 30-26-36. These strains are characterized by high biomass yield, ability to accumulate high amounts of lipids, and their potential as producers of dietetically important fatty acids. The aim of this work was the production of nutritionally important fatty acids by utilization of n-alkanes with an odd number of carbon atoms, alone and in combination with glucose and subsequent analysis of microbial lipids accumulation and fatty acid profile...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/29127615/fungal-and-parasitic-cns-infections
#19
Pratibha Singhi, Arushi Gahlot Saini
Central nervous system fungal infections can be broadly divided into those that infect a healthy host such as Cryptococcus, Coccidioides, Histoplasma, Blastomyces, Sporothrix spp., and those that cause opportunistic infections in an immunocompromised host such as Candida, Aspergillus, Zygomycetes, Trichosporon spp. The clinical manifestations of central nervous system fungal infections commonly seen in children in clinical practice include a chronic meningitis or meningoencephalitis syndrome, brain abscess, rhino-cerebral syndrome and rarely, a fungal ventriculitis...
November 11, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/29097125/chemical-and-biological-conversion-of-crude-glycerol-derived-from-waste-cooking-oil-to-biodiesel
#20
Jiaxin Chen, Song Yan, Xiaolei Zhang, Rajeshwar Dayal Tyagi, Rao Y Surampalli, J R Valéro
In this study, crude, purified, and pure glycerol were used to cultivate Trichosporon oleaginosus for lipid production which was then used as feedstock of biodiesel production. The purified glycerol was obtained from crude glycerol by removing soap with addition of H3PO4 which converted soap to free fatty acids and then separated from the solution. The results showed that purified glycerol provided similar performance as pure glycerol in lipid accumulation; however, crude glycerol as carbon source had negatively impacted the lipid production of T...
October 30, 2017: Waste Management
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