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https://www.readbyqxmd.com/read/28098791/development-of-high-throughput-method-for-measurement-of-vascular-nitric-oxide-generation-in-microplate-reader
#1
Soad S Abd El-Hay, Christa L Colyer
BACKGROUND: Despite the importance of nitric oxide (NO) in vascular physiology and pathology, a high-throughput method for the quantification of its vascular generation is lacking. OBJECTIVE: By using the fluorescent probe 4-amino-5-methylamino-2',7'-difluorofluorescein (DAF-FM), we have optimized a simple method for the determination of the generation of endothelial nitric oxide in a microplate format. METHODS: A nitric oxide donor was used (3-morpholinosydnonimine hydrochloride, SIN-1)...
January 13, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28098781/distinct-neurotoxicity-profile-of-listeriolysin-o-from-listeria-monocytogenes
#2
Jana Maurer, Sabrina Hupp, Carolin Bischoff, Christina Foertsch, Timothy J Mitchell, Trinad Chakraborty, Asparouh I Iliev
Cholesterol-dependent cytolysins (CDCs) are protein toxins that originate from Gram-positive bacteria and contribute substantially to their pathogenicity. CDCs bind membrane cholesterol and build prepores and lytic pores. Some effects of the toxins are observed in non-lytic concentrations. Two pathogens, Streptococcus pneumoniae and Listeria monocytogenes, cause fatal bacterial meningitis, and both produce toxins of the CDC family-pneumolysin and listeriolysin O, respectively. It has been demonstrated that pneumolysin produces dendritic varicosities (dendrite swellings) and dendritic spine collapse in the mouse neocortex, followed by synaptic loss and astrocyte cell shape remodeling without elevated cell death...
January 13, 2017: Toxins
https://www.readbyqxmd.com/read/28098741/does-familial-aggregation-of-chronic-low-back-pain-impact-on-recovery-a-population-based-twin-study
#3
J R Zadro, D Shirley, J F Sanchez-Romera, J R Ordoñana, P H Ferreira
STUDY DESIGN: Longitudinal twin-cohort study. OBJECTIVE: To investigate the impact familial aggregation of chronic low back pain (LBP) has on the recovery from chronic LBP. SUMMARY OF BACKGROUND DATA: LBP is a worldwide problem, with pain and disability often becoming chronic. Genetics and familial behaviours could significantly impact the recovery from chronic LBP but have not been extensively investigated. METHODS: 624 Spanish twins from the Murcia Twin Registry reported experiencing chronic LBP within the past two years during the 2009/11 data collection wave and were followed up in 2013...
January 16, 2017: Spine
https://www.readbyqxmd.com/read/28098524/swine-influenza-virus-h1n2-characterization-and-transmission-in-ferrets-chile
#4
Nicolás Bravo-Vasquez, Erik A Karlsson, Pedro Jimenez-Bluhm, Victoria Meliopoulos, Bryan Kaplan, Shauna Marvin, Valerie Cortez, Pamela Freiden, Melinda A Beck, Christopher Hamilton-West, Stacey Schultz-Cherry
Phylogenetic analysis of the influenza hemagglutinin gene (HA) has suggested that commercial pigs in Chile harbor unique human seasonal H1-like influenza viruses, but further information, including characterization of these viruses, was unavailable. We isolated influenza virus (H1N2) from a swine in a backyard production farm in Central Chile and demonstrated that the HA gene was identical to that in a previous report. Its HA and neuraminidase genes were most similar to human H1 and N2 viruses from the early 1990s and internal segments were similar to influenza A(H1N1)pdm09 virus...
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28098398/depression-and-discrimination-in-the-lives-of-women-transgender-and-gender-liminal-people-in-ontario-canada
#5
Charmaine C Williams, Deone Curling, Leah S Steele, Margaret F Gibson, Andrea Daley, Datejie Cheko Green, Lori E Ross
This article uses an intersectionality lens to explore how experiences of race, gender, sexuality, class and their intersections are associated with depression and unmet need for mental healthcare in a population of 704 women and transgender/gender liminal people from Ontario, Canada. A survey collecting demographic information, information about mental health and use of mental healthcare services, and data for the Everyday Discrimination Scale and the PHQ-9 Questionnaire for Depression was completed by 704 people via Internet or pen-and-paper between June 2011 and June 2012...
January 18, 2017: Health & Social Care in the Community
https://www.readbyqxmd.com/read/28097672/collective-resistance-despite-complicity-high-identifiers-rise-above-the-legitimization-of-disadvantage-by-the-in-group
#6
Gloria Jiménez-Moya, Rosa Rodríguez-Bailón, Russell Spears, Soledad de Lemus
How do individuals deal with group disadvantage when their fellow in-group members conceive it as legitimate? Integrating research on the normative conflict model (Packer, 2008, Pers. Soc. Psychol. Rev., 12, 50) and collective action, we expect high identifiers to reject the in-group norm of legitimacy that justifies the inequality, and to assert that the group is actually able and willing to contest the disadvantage by collective means. In Study 1 and Study 2, we tested this hypothesis in different intergroup contexts...
January 17, 2017: British Journal of Social Psychology
https://www.readbyqxmd.com/read/28097594/mechanism-of-anti-vibrio-activity-of-marine-probiotic-strain-bacillus-pumilus-h2-and-characterization-of-the-active-substance
#7
Xi-Yan Gao, Ying Liu, Li-Li Miao, Er-Wei Li, Ting-Ting Hou, Zhi-Pei Liu
Vibriosis is a major epizootic disease that impacts free-living and farmed fish species worldwide. Use of probiotics is a promising approach for prevention of Vibrio infections in aquaculture. A probiotic anti-Vibrio strain, Bacillus pumilus H2, was characterized, and the mechanism of its effect was investigated. All 29 Vibrio strains tested were growth-inhibited by H2. The anti-Vibrio substance present in cell-free supernatant of H2 was purified and characterized by reversed-phase HPLC. Minimum inhibitory concentrations of the purified substance, determined in liquid media for various Vibrio strains, ranged from 0...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28097516/are-event-related-potentials-to-dynamic-facial-expressions-of-emotion-related-to-individual-differences-in-the-accuracy-of-processing-facial-expressions-and-identity
#8
Guillermo Recio, Oliver Wilhelm, Werner Sommer, Andrea Hildebrandt
Despite a wealth of knowledge about the neural mechanisms behind emotional facial expression processing, little is known about how they relate to individual differences in social cognition abilities. We studied individual differences in the event-related potentials (ERPs) elicited by dynamic facial expressions. First, we assessed the latent structure of the ERPs, reflecting structural face processing in the N170, and the allocation of processing resources and reflexive attention to emotionally salient stimuli, in the early posterior negativity (EPN) and the late positive complex (LPC)...
January 17, 2017: Cognitive, Affective & Behavioral Neuroscience
https://www.readbyqxmd.com/read/28097387/all-dual-mobility-cups-are-not-the-same
#9
Thierry Aslanian
PURPOSE: Although the natural history of dual mobility has been exclusively borne for 20 years by a single company (due to industrial ownership), the concept has undeniably been very widely popularised with nearly 40 cups on the French market which should be regarded as a weight bearing surface, both broadly and in their own right. However, within the same original idea, these implants are not all identical (design, material, fixation ect.).The aim of this work is to propose a classification of different dual mobility cups by distinguishing between thegeneral characteristics of a conventional cup and those particular to this type of implant...
January 18, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28097054/hydrogel-scaffolds-promote-neural-gene-expression-and-structural-reorganization-in-human-astrocyte-cultures
#10
V Bleu Knight, Elba E Serrano
Biomaterial scaffolds have the potential to enhance neuronal development and regeneration. Understanding the genetic responses of astrocytes and neurons to biomaterials could facilitate the development of synthetic environments that enable the specification of neural tissue organization with engineered scaffolds. In this study, we used high throughput transcriptomic and imaging methods to determine the impact of a hydrogel, PuraMatrix™, on human glial cells in vitro. Parallel studies were undertaken with cells grown in a monolayer environment on tissue culture polystyrene...
2017: PeerJ
https://www.readbyqxmd.com/read/28096975/a-prospective-survey-of-pseudomonas-aeruginosa-colonization-and-infection-in-the-intensive-care-unit
#11
Regev Cohen, Frida Babushkin, Shoshana Cohen, Marina Afraimov, Maurice Shapiro, Martina Uda, Efrat Khabra, Amos Adler, Ronen Ben Ami, Svetlana Paikin
BACKGROUND: Pseudomonas aeruginosa (PA) surveillance may improve empiric antimicrobial therapy, since colonizing strains frequently cause infections. This colonization may be 'endogenous' or 'exogenous', and the source determines infection control measures. We prospectively investigated the sources of PA, the clinical impact of PA colonization upon admission and the dynamics of colonization at different body sites throughout the intensive care unit stay. METHODS: Intensive care patients were screened on admission and weekly from the pharynx, endotracheal aspirate, rectum and urine...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28096972/objects-versus-shadows-as-influences-on-perceived-object-motion
#12
Marouane Ouhnana, Frederick A A Kingdom
The motion trajectory of an object's cast shadow has been shown to alter the perceived trajectory of a casting object, an effect that holds even if the cast shadow appears unrealistic. This raises the question of whether a cast shadow per se is necessary for this influence, a question that has been studied only with stationary targets. We examined the relative influence of a shadow and a spherical object on the perceived motion trajectory of an identical spherical object, using a paradigm similar to Kersten, Mamassian, and Knill's ball-in-box animation...
November 2016: I-Perception
https://www.readbyqxmd.com/read/28096862/does-nosema-ceranae-wipe-out-nosema-apis-in-turkey
#13
Rahşan Ivgin Tunca, Devrim Oskay, Ayhan Gosterit, Olgay Kaan Tekin
BACKGROUND: The aim of this study was to determine the prevalence of the Nosema ceranae and Nosema apis among apiaries using both spore counts and multiplex PCR and the replacement of N. apis by N. ceranae in some regions of Turkey. METHODS: A hundred honey bee samples were collected from 99 apiaries in 11 different locations in 2011-2012 in Turkey. Nosema infection degree from collected samples was determined using light microscope and molecular detection of Nosema spp...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096621/multiple-infections-of-anaplasma-platys-variants-in-philippine-dogs
#14
Adrian Patalinghug Ybañez, Rochelle Haidee Daclan Ybañez, Naoaki Yokoyama, Hisashi Inokuma
AIM: Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs. MATERIALS AND METHODS: DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR)...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096466/a-phosphoproteomic-screen-identifies-a-guanine-nucleotide-exchange-factor-for-rab3a-as-a-map-kinase-phosphatase-5-regulated-map-kinase-target-in-il-6-secretion-and-myogenesis
#15
Hojin Lee, Kisuk Min, Jae-Sung Yi, Hao Shi, Woochul Chang, Leandra Jackson, Anton M Bennett
The mitogen-activated protein kinases (MAPKs) have been shown to regulate skeletal muscle function. Previously, we showed that MAPK phosphatase-5 (MKP-5) negatively regulates myogenesis and regeneration of skeletal muscle through inhibition of p38 MAPK and c-Jun N-terminal kinase (JNK). However, the identity and contribution of MKP-5-dependent MAPK targets in the regulation of skeletal muscle function and regenerative myogenesis has not been established. In order to identify MKP-5-dependent MAPK substrates in skeletal muscle, we performed a global differential phospho-MAPK substrate screen in regenerating skeletal muscles of wild type and MKP-5-deficient mice...
January 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28096351/uncovering-hidden-variation-in-polyploid-wheat
#16
Ksenia V Krasileva, Hans A Vasquez-Gross, Tyson Howell, Paul Bailey, Francine Paraiso, Leah Clissold, James Simmonds, Ricardo H Ramirez-Gonzalez, Xiaodong Wang, Philippa Borrill, Christine Fosker, Sarah Ayling, Andrew L Phillips, Cristobal Uauy, Jorge Dubcovsky
Comprehensive reverse genetic resources, which have been key to understanding gene function in diploid model organisms, are missing in many polyploid crops. Young polyploid species such as wheat, which was domesticated less than 10,000 y ago, have high levels of sequence identity among subgenomes that mask the effects of recessive alleles. Such redundancy reduces the probability of selection of favorable mutations during natural or human selection, but also allows wheat to tolerate high densities of induced mutations...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28096211/primate-embryogenesis-predicts-the-hallmarks-of-human-na%C3%A3-ve-pluripotency
#17
REVIEW
Thorsten Boroviak, Jennifer Nichols
Naïve pluripotent mouse embryonic stem cells (ESCs) resemble the preimplantation epiblast and efficiently contribute to chimaeras. Primate ESCs correspond to the postimplantation embryo and fail to resume development in chimaeric assays. Recent data suggest that human ESCs can be 'reset' to an earlier developmental stage, but their functional capacity remains ill defined. Here, we discuss how the naïve state is inherently linked to preimplantation epiblast identity in the embryo. We hypothesise that distinctive features of primate development provide stringent criteria to evaluate naïve pluripotency in human and other primate cells...
January 15, 2017: Development
https://www.readbyqxmd.com/read/28095951/simulated-microgravity-effects-on-nonsmall-cell-lung-cancer-cell-proliferation-and-migration
#18
Jae Ho Chung, Chi Bum Ahn, Kuk Hui Son, Eunjue Yi, Ho Sung Son, Han-Sung Kim, Sung Ho Lee
BACKGROUND: Despite improvements in medical technology, lung cancer metastasis remains a global health problem. The effects of microgravity on cell morphology, structure, functions, and their mechanisms have been widely studied; however, the biological effects of simulated microgravity on the interaction between cells and its eventual influence on the characteristics of cancer cells are yet to be discovered. We examined the effects of simulated microgravity on the metastatic ability of different lung cancer cells using a random positioning machine...
February 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28095868/pik3ca-mutations-are-common-in-lobular-carcinoma-in-situ-but-are-not-a-biomarker-of-progression
#19
Vandna Shah, Salpie Nowinski, Dina Levi, Irek Shinomiya, Narda Kebaier Ep Chaabouni, Cheryl Gillett, Anita Grigoriadis, Trevor A Graham, Rebecca Roylance, Michael A Simpson, Sarah E Pinder, Elinor J Sawyer
BACKGROUND: Lobular carcinoma in situ (LCIS) is a non-invasive breast lesion that is typically found incidentally on biopsy and is often associated with invasive lobular carcinoma (ILC). LCIS is considered by some to be a risk factor for future breast cancer rather than a true precursor lesion. The aim of this study was to identify genetic changes that could be used as biomarkers of progression of LCIS to invasive disease using cases of pure LCIS and comparing their genetic profiles to LCIS which presented contemporaneously with associated ILC, on the hypothesis that the latter represents LCIS that has already progressed...
January 17, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28095818/when-parents-won-t-vaccinate-their-children-a-qualitative-investigation-of-australian-primary-care-providers-experiences
#20
Nina J Berry, Alexandra Henry, Margie Danchin, Lyndal J Trevena, Harold W Willaby, Julie Leask
BACKGROUND: Increasingly, the experiences and perceptions of parents who decline vaccination are the subject of investigation. However, the experiences of clinicians who encounter these parents in the course of their work has received little academic attention to date. This study aimed to understand the challenges faced and strategies used when general practitioners and immunising nurses encounter parents who choose not to vaccinate their children. METHODS: Primary care providers were recruited from regions identified through the Australian Childhood Immunisation Register (ACIR) as having higher than national average rates of registered objection to childhood vaccination...
January 17, 2017: BMC Pediatrics
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