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In vitro fertilization

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https://www.readbyqxmd.com/read/29925074/a-retrospective-study-of-letrozole-treatment-prior-to-human-chorionic-gonadotropin-in-women-with-polycystic-ovary-syndrome-undergoing-in-vitro-fertilization-at-risk-of-ovarian-hyperstimulation-syndrome
#1
Yilu Chen, Tanchu Yang, Cuifang Hao, Junzhao Zhao
BACKGROUND Women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF) are given letrozole before a trigger injection of human chorionic gonadotropin (hCG) to lower estrogen (E2) levels, but can experience ovarian hyperstimulation syndrome (OHSS). The aim of this study was to evaluate the effect of oral letrozole, prior to administration of hCG, on the outcome of IVF and development of OHSS. MATERIAL AND METHODS Retrospective clinical review included 181 cases of women with PCOS who underwent IVF cycles with intracytoplasmic sperm injection (ICSI) and embryo transfer (ET) (IVF/ICSI-ET)...
June 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29924852/exposure-to-elevated-glucose-concentrations-alters-the-metabolomic-profile-of-bovine-blastocysts
#2
Karen Uhde, Helena T A van Tol, Tom A E Stout, Bernard A J Roelen
Chronically high blood glucose concentrations are a characteristic of diabetes mellitus. Maternal diabetes affects the metabolism of early embryos and can cause a delay in development. To mimic maternal diabetes, bovine in vitro fertilization and embryo culture were performed in fertilization medium and culture medium containing 0.5, 2, 3, and 5 mM, glucose whereas under control conditions, the medium was glucose free (0 mM). Compared to control conditions (0 mM, 31%), blastocyst development was decreased to 23% with 0...
2018: PloS One
https://www.readbyqxmd.com/read/29917282/parental-conformity-expectations-effect-on-twins-and-singletons-parent-adolescent-relationships-associations-with-change-in-adjustment-from-middle-childhood-to-adolescence
#3
Kayla N Anderson, Martha A Rueter, Jennifer J Connor, Ascan F Koerner
Growing use of in vitro fertilization (IVF) has resulted in an elevated twin birth rate, and a burgeoning population of IVF twins who are now aging into adolescence and beyond. This study tests a model examining whether parental conformity expectations have differential effects on twins' versus singletons' parent-adolescent relationship satisfaction, and if this effect is indirectly associated with relative changes in twins' and singletons' internalizing and externalizing behavior from middle childhood to adolescence...
June 19, 2018: Journal of Research on Adolescence: the Official Journal of the Society for Research on Adolescence
https://www.readbyqxmd.com/read/29916276/association-of-estrogen-progesterone-and-follicle-stimulating-hormone-receptor-polymorphisms-with-in-vitro-fertilization-outcomes
#4
Vijaya Ganesh, Vettriselvi Venkatesan, Teena Koshy, Sanjeeva Nellapalli Reddy, Suruli Muthumuthiah, Solomon Franklin Durairaj Paul
Despite the advances in in vitro fertilization (IVF), the implantation success rate for infertile women remains approximately only 15%. In this study, we sought to determine whether implantation failure after repeated IVF treatments is influenced by the presence of common variants in estrogen α, progesterone and follicle stimulating hormone receptor genes. The study population included three groups of women: group 1 were 50 women who had the transfer of ≥3 high-quality embryos during the IVF procedure without ever having had a clinical pregnancy; group 2 were 50 women who achieved a clinical pregnancy after ≤3 high-quality embryos transfers and group 3 were 50 control subjects who achieved a clinical pregnancy without any fertility therapy that resulted in a one live-born infant...
June 19, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29916218/pharmacogenetic-algorithm-for-individualized-controlled-ovarian-stimulation-icos-in-assisted-reproductive-technology-cycles
#5
Matheus Roque, Bianca Bianco, Denise M Christofolini, Emerson B Cordts, Fabia L Vilarino, Waldemar Carvalho, Marcello Valle, Marcos Sampaio, Selmo Geber, Sandro C Esteves, Caio P Barbosa
Controlled ovarian stimulation (COS) is crucial for optimizing in vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) success. Multiple factors influence the ovarian response to COS, making predictions about oocyte yields not so straightforward. As a result, the ovarian response may be poor or suboptimal, or even excessive, all of which have negative consequences for the affected patient. There is a group of patients that present with a suboptimal response to COS despite normal biomarkers of ovarian reserve, such as AFC and AMH...
June 14, 2018: Panminerva Medica
https://www.readbyqxmd.com/read/29915225/preimplantation-genetic-screening-with-spent-culture-medium-blastocoel-fluid-for-in-vitro-fertilization
#6
Penghao Li, Zhe Song, Yaxin Yao, Tianhua Huang, Rurong Mao, Jun Huang, Yongyi Ma, Xin Dong, Wenlong Huang, Jihua Huang, Tianjian Chen, Ting Qu, Lingxiao Li, Ying Zhong, Jiang Gu
Preimplantation genetic screening (PGS) detects chromosomal aneuploidy from DNA extracted from trophectodermal biopsy of the embryos before implantation. Although a controlled study showed no difference in pregnancy rates between this invasive cell biopsy technique and a non-biopsied control group, the potential long-term damage by the current PGS method has not be completely ruled out. We therefore tested a less-invasive protocol which utilizes spent culture medium combining with blastocoel fluid (ECB) to assess chromosomal aneuploidy...
June 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29915110/nitrogenase-activity-oxygen-exposure-and-hydrogen-production-by-azotobacter-vinelandii
#7
Jace Natzke, Jesse Noar, José M Bruno-Bárcena
Azotobacter vinelandii selectively utilizes three types of nitrogenase (molybdenum, vanadium, and iron-only) to fix N2 with their expression regulated by the presence or absence of different metal cofactors in its environment. Each alternative nitrogenase isoenzyme is predicted to have different electron flux requirements based off in vitro measurements, with the molybdenum nitrogenase requiring the lowest flux and the iron-only nitrogenase requiring the highest. Here, prior characterized strains, de-repressed in nitrogenase synthesis and also deficient in uptake hydrogenase, were further modified to generate new mutants lacking the ability to produce poly-β-hydroxybutyrate (PHB)...
June 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29914496/outcomes-of-neonates-born-following-transfers-of-frozen-thawed-cleavage-stage-embryos-with-blastomere-loss-a-prospective-multicenter-cohort-study
#8
Yan-Ting Wu, Cheng Li, Yi-Min Zhu, Shu-Hua Zou, Qiong-Fang Wu, Li-Ping Wang, Yan Wu, Rong Yin, Chao-Yi Shi, Jing Lin, Zi-Ru Jiang, Yi-Jing Xu, Yun-Fei Su, Jian Zhang, Jian-Zhong Sheng, William D Fraser, Zhi-Wei Liu, He-Feng Huang
BACKGROUND: Despite limited information on neonatal safety, the transfer of frozen-thawed cleavage-stage embryos with blastomere loss is common in women undergoing in vitro fertilization. We aimed to evaluate the pregnancy outcomes and safety of frozen-thawed cleavage-stage embryos with blastomere loss. METHODS: This prospective, multicenter, cohort study included all frozen-thawed cleavage-stage embryo transfer (FET) cycles between 2002 and 2012. Pregnancy outcomes and subsequent neonatal outcomes were compared between FET cycles with intact embryos and those with blastomere loss...
June 19, 2018: BMC Medicine
https://www.readbyqxmd.com/read/29914391/in-vitro-mineral-nutrition-of-curcuma-longa-l-affects-production-of-volatile-compounds-in-rhizomes-after-transfer-to-the-greenhouse
#9
Rabia F El-Hawaz, Mary H Grace, Alan Janbey, Mary Ann Lila, Jeffrey W Adelberg
BACKGROUND: Turmeric is a rich source of bioactive compounds useful in both medicine and cuisine. Mineral concentrations effects (PO4 3- , Ca2+ , Mg2+ , and KNO3 ) were tested during in vitro rhizome development on the ex vitro content of volatile constituents in rhizomes after 6 months in the greenhouse. A response surface method (D-optimal criteria) was repeated in both high and low-input fertilizer treatments. Control plants were grown on Murashige and Skoog (MS) medium, acclimatized in the greenhouse and grown in the field...
June 18, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29913423/effect-of-aminopeptidase-n-on-functions-and-fertility-of-mouse-spermatozoa-in-vitro
#10
Amena Khatun, Kyu-Ho Kang, Do-Yeal Ryu, Md Saidur Rahman, Woo-Sung Kwon, Myung-Geol Pang
Aminopeptidase N (APN) is defined as a multifunctional enzyme, which regulate cellular physiology of a wide variety of cells in human. Earlier studies reported that mammalian semen shares this common enzyme as a major protein of seminal plasma that has correlation with male fertility, while the regulatory mechanisms of APN in spermatozoa are still far from being well understood. Present study was designed to investigate the role of APN in biological and chemical functions of spermatozoa using an in vitro antagonistic approach...
June 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29912374/luteal-phase-anovulatory-follicles-result-in-the-production-of-competent-oocytes-intra-patient-paired-case-control-study-comparing-follicular-versus-luteal-phase-stimulations-in-the-same-ovarian-cycle
#11
Danilo Cimadomo, Alberto Vaiarelli, Silvia Colamaria, Elisabetta Trabucco, Carlo Alviggi, Roberta Venturella, Erminia Alviggi, Ramona Carmelo, Laura Rienzi, Filippo Maria Ubaldi
STUDY QUESTION: Are the mean numbers of blastocysts obtained from sibling cohorts of oocytes recruited after follicular phase and luteal phase stimulations (FPS and LPS) in the same ovarian cycle similar? SUMMARY ANSWER: The cohorts of oocytes obtained after LPS are larger than their paired-FPS-derived cohorts and show a comparable competence, thus resulting in a larger mean number of blastocysts. WHAT IS KNOWN ALREADY: Three theories of follicle recruitment have been postulated to date: (i) the 'continuous recruitment' theory, (ii) the 'single recruitment episode' theory and (iii) the 'wave' theory...
June 15, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29911897/per-and-polyfluoroalkyl-substances-impact-human-spermatogenesis-in-a-stem-cell-derived-model
#12
Alyse N Steves, Adam Turry, Brittany Gill, Danielle Clarkson-Townsend, Joshua M Bradner, Ian Bachli, W Michael Caudle, Gary W Miller, Anthony W S Chan, Charles A Easley
Per- and polyfluoroalkyl substances (PFASs) represent a highly ubiquitous group of synthetic chemicals used in products ranging from water and oil repellents and lubricants to firefighting foam. These substances can enter and accumulate in multiple tissue matrices in up to 100% of people assessed. Though animal models strongly identify these compounds as male reproductive toxicants, with exposed rodents experiencing declines in sperm count, alterations in hormones, and DNA damage in spermatids, among other adverse outcomes, human studies report conflicting conclusions as to the reproductive toxicity of these chemicals...
June 18, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29910214/effect-of-a-single-epidural-administration-of-follicle-stimulating-hormone-via-caudal-vertebrae-on-superstimulation-for-in-vivo-and-in-vitro-embryo-production-in-japanese-black-cows
#13
Kenichiro Sakaguchi, Atsushi Ideta, Yojiro Yanagawa, Masashi Nagano, Seiji Katagiri, Masato Konishi
Here, we describe a simplified procedure for embryo production in the Japanese black cow that uses a single caudal epidural injection of follicle-stimulating hormone (FSH). First, we compared the efficiency of superovulation for in vivo embryo production between conventional multiple FSH treatment (control, n = 10) and single epidural administration (epidural, n = 5). The number of transferable blastocysts was similar between control and epidural groups (4.7 ± 3.5 and 9.0 ± 6.0, respectively). Next, we compared in vitro embryo production by ovum pick-up and in vitro fertilization (OPU-IVF) between control (n = 12) and epidural groups (n = 12)...
June 16, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29909692/serum-oxytocin-profiles-in-patients-with-repeated-implantation-failure-during-ivf-cycles
#14
Tingting Xia, Qiuli Liu, Qingjian Ye, Weijie Xing, Dong Wang, Jie Li, Zhi-Jun Zang
OBJECTIVE: The objective of this study is to investigate the association between oxytocin (OT) levels and repeated implantation failure (RIF) during in vitro fertilization-embryo transfer (IVF-ET) cycles. METHODS: Blood samples were collected from 108 women undergoing IVF-ET treatment at the following time points: gonadotrophin (Gn) administration day (Gn Day 0), hCG administration day (hCG Day 0), ET administration day (ET Day 0), and 5 d after ET (ET Day 5)...
June 17, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29909068/effects-of-histone-methyltransferase-inhibitor-chaetocin-on-histone-h3k9-methylation-of-cultured-ovine-somatic-cells-and-development-of-preimplantation-cloned-embryos
#15
Yu-Mei Zhang, En-En Gao, Qian-Qian Wang, Hao Tian, Jian Hou
Aberrant hypermethylation of histone H3 lysine 9 (H3K9) is a key barrier to the development of cloned embryos by somatic cell nuclear transfer (SCNT). The objective of this study was to assess the effects of chaetocin, an inhibitor of H3K9 methyltransferase SUV39 H, in regulating the H3K9 methylation in ovine SCNT embryos. Treatment of sheep fetal fibroblast cells with chaetocin specifically decreased the levels of H3K9 di-and trimethylation, and down-regulated the expression of H3K9 methyltransferases, SUV39H1/2 and G9A...
June 14, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29908951/factors-associated-with-pregnancy-after-in-vitro-fertilization-in-infertile-patients-with-posterior-deep-pelvic-endometriosis-a-retrospective-study
#16
C Rubod, A Fouquet, S Bartolo, J Lepage, A Capelle, C Lefebvre, E Kamus, D Dewailly, P Collinet
INTRODUCTION: The management of posterior deep endometriosis is not consensual. This is due to a great heterogeneity of data from the literature. Many series were small and overall, predictors of pregnancy were not all included and analyzed by multivariate analysis. We conducted this study to evaluate the factors associated with pregnancy during the first two in vitro fertilization (IVF) attempts in infertile women with posterior deep endometriosis. MATERIAL AND METHODS: 230 women were included in this retrospective observational study, between January 1th, 2007 and September 30th, 2013, at the University Hospital of Lille...
June 14, 2018: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/29908776/effects-of-chronic-endometritis-therapy-on-in-vitro-fertilization-outcome-in-women-with-repeated-implantation-failure-a-systematic-review-and-meta-analysis
#17
Amerigo Vitagliano, Carlo Saccardi, Marco Noventa, Attilio Di Spiezio Sardo, Gabriele Saccone, Ettore Cicinelli, Sara Pizzi, Alessandra Andrisani, Pietro Salvatore Litta
OBJECTIVE: To evaluate the impact of antibiotic therapy for chronic endometritis (CE) on IVF outcome. DESIGN: Systematic review and meta-analysis. SETTING: Not applicable. PATIENT(S): Infertile women with history of recurrent implantation failure, defined as two or more failed ETs, undergoing one or more IVF cycle(s). INTERVENTION(S): The review was registered in PROSPERO (CRD42017062494) before the start of the literature search...
June 13, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29908772/first-in-vitro-fertilization-baby-this-is-how-it-happened
#18
REVIEW
Simon Fishel
On July 25, 1978, the first human was born following extracorporeal fertilization, an event that opened up a new medical science: expanding our knowledge of and developing novel treatments for infertility, radically changing the opportunities for families with inherited monogenic disorders, generating the new discipline of clinical embryology, and paving the way for studies into stem cell biology. In vitro fertilization (IVF), as it became known in its simplest form, went even further: it engaged the myriad of minds in human society...
June 13, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29908770/pregnancy-outcomes-from-more-than-1-800-in-vitro-fertilization-cycles-with-the-use-of-24-chromosome-single-nucleotide-polymorphism-based-preimplantation-genetic-testing-for-aneuploidy
#19
Alexander L Simon, Michelle Kiehl, Erin Fischer, J Glenn Proctor, Mark R Bush, Carolyn Givens, Matthew Rabinowitz, Zachary P Demko
OBJECTIVE: To measure in vitro fertilization (IVF) outcomes following 24-chromosome single‒nucleotide-polymorphism (SNP)-based preimplantation genetic testing for aneuploidy (PGT-A) and euploid embryo transfer. DESIGN: Retrospective. SETTING: Fertility clinics and laboratory. PATIENT(S): Women 20-46 years of age undergoing IVF treatment. INTERVENTION(S): Twenty-four-chromosome SNP-based PGT-A of day 5/6 embryo biopsies...
June 13, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29907333/superstimulation-with-ecg-prior-to-ovum-pick-up-improves-follicular-development-and-fertilization-rate-of-cattle-oocytes
#20
Bibiana Noal Ribas, Daniele Missio, Isac Junior Roman, Normélio Alves Neto, Izaias Claro, Daniela Dos Santos Brum, Fabio Gallas Leivas
This study aimed to evaluate the effect of equine chorionic gonadotrophin (eCG) stimulation prior to ovum pick-up (OPU) on follicular development, number and quality of recovered oocytes, fertilization rate, and early embryo development in vitro. There were four OPU sessions (cross over) conducted on 16 Braford cows to evaluate the effect of various eCG doses. The timing of the wave of ovarian follicular development was synchronized, and three days after, the respective eCG dose was administrated (0, 200, 400, or 800 IU)...
June 9, 2018: Animal Reproduction Science
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