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In vitro fertilization

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https://www.readbyqxmd.com/read/28319264/a-retrospective-study-of-the-effect-of-increasing-age-on-success-rates-of-assisted-reproductive-technology
#1
Yvonne M O'Brien, Michael Ryan, Fiona Martyn, Mary B Wingfield
OBJECTIVE: To demonstrate the effect of increasing age on the outcome of assisted reproductive technology, particularly among women aged 40 years or older. METHODS: A retrospective analysis was conducted using prospectively collected data for all in vitro fertilization and intracytoplasmic sperm injection cycles among women aged 30-35 years or 40-44 years conducted at Merrion Fertility Clinic, Dublin, Ireland, between January 1, 2010, and December 31, 2014. The relationship between age and treatment outcome was assessed...
March 20, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28315625/endometrial-injury-for-women-with-previous-in-vitro-fertilization-failure-does-it-improve-pregnancy-rate
#2
(no author information available yet)
Implantation failure is one of the major factors limiting success of in vitro fertilization (IVF) treatment. According to ESHRE 2009 data only 32% of fresh embryo transfers resulted in clinical pregnancies. There are many ideas to improve the treatment outcomes, endometrial injury being one of them. It has been suggested that local endometrial injury, performed either by pipelle biopsy or hysteroscopy, may increase clinical pregnancy rate. However, up to date literature is widely disparate on that subject. There is no conclusion with regard to optimal timing, the number and technique of the procedure...
November 19, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28306561/diving-into-the-oocyte-pool
#3
Stine G Kristensen, Susanne E Pors, Claus Y Andersen
PURPOSE OF REVIEW: The ovarian reserve comprises an enormous surplus of follicles. Despite this, some women produce insufficient numbers of oocytes by conventional fertility treatments. However, recent technical accomplishments may transform assisted reproductive technology (ART) in such a way that oocytes are not necessarily the limiting factor. In this review, we present possible new strategies for enhancing the quantity of mature oocytes, and current views on autologous oocytes as potential sources of mitochondria to lift performance of compromised oocytes...
March 16, 2017: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28306344/live-birth-after-replacement-of-an-embryo-obtained-from-a-spontaneously-in-vitro-matured-metaphase-i-oocyte
#4
Paloma Piqueras, Miguel Gallardo, María Hebles, Juan Manuel Jiménez, Beatriz Migueles, Lorena Montero, Fernando Sánchez-Martín, Pascual Sánchez-Martín
This case report describes a live birth after the fresh replacement of an embryo obtained from a spontaneously in vitro matured oocyte. The patient was subjected to controlled ovarian stimulation for IVF treatment, obtaining two oocytes. One was found to be immature at the time of denudation, at metaphase-I. This immature oocyte was kept in culture overnight in standard conditions along with the second oocyte - which was mature but failed to fertilize - spontaneously achieving metaphase-II, and was subjected to ICSI...
March 17, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28302936/cryotolerance-of-porcine-blastocysts-is-improved-by-treating-in-vitro-matured-oocytes-with-l-carnitine-prior-to-fertilization
#5
Jenna L Lowe, Louise K Bartolac, Roslyn Bathgate, Christopher G Grupen
Sufficient generation of adenosine triphosphate (ATP) by oocytes is critical for fertilization and embryo development. The objective of this study was to determine the effects of supplementing media with L-carnitine, a co-factor required for the metabolism of fatty acids, during the peri-fertilization period on embryo development and energy generation. Firstly, in vitro fertilization (IVM) porcine oocytes were co-incubated with sperm in IVF medium supplemented with 0‒24 mM L-carnitine. The blastocyst formation rate of the control group was greater than those of the L-carnitine groups (P < 0...
March 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28300168/zebrafish-caudal-haematopoietic-embryonic-stromal-tissue-chest-cells-support-haematopoiesis
#6
Anja Wolf, Julian Aggio, Clyde Campbell, Francis Wright, Gabriel Marquez, David Traver, David L Stachura
Haematopoiesis is an essential process in early vertebrate development that occurs in different distinct spatial locations in the embryo that shift over time. These different sites have distinct functions: in some anatomical locations specific hematopoietic stem and progenitor cells (HSPCs) are generated de novo. In others, HSPCs expand. HSPCs differentiate and renew in other locations, ensuring homeostatic maintenance. These niches primarily control haematopoiesis through a combination of cell-to-cell signalling and cytokine secretion that elicit unique biological effects in progenitors...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299785/should-obese-women-s-access-to-assisted-fertility-treatment-be-limited-a-scientific-and-ethical-analysis
#7
Kelton Tremellen, Dominic Wilkinson, Julian Savulescu
Obesity is associated with a reduction in fertility treatment success and increased risks to mother and child. Therefore guidelines of the Royal Australian and New Zealand College of Obstetricians and Gynaecologists (RANZCOG) suggest that a body mass index exceeding 35 kg/m(2) should be an absolute contraindication to assisted fertility treatment such as in vitro fertilisation IVF. In this paper we challenge the ethical and scientific basis for such a ban. Livebirth rates for severely obese women are reduced by up to 30%, but this result is still far better than that observed for many older women who are allowed access to IVF...
March 16, 2017: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/28299549/elective-single-blastocyst-transfer-in-advanced-maternal-age
#8
Samer Tannus, Weon-Young Son, Michael Haim Dahan
PURPOSE: The purpose of this study was to investigate reproductive outcomes following elective single blastocyst transfer (eSBT) compared with those of double blastocyst transfer (DBT) in advanced maternal age. METHODS: This was a retrospective cohort study performed at an academic fertility center. All women aged 40 and over for whom in vitro fertilization (IVF) cycles were performed and in whom embryo culture was extended to the blastocyst stage were reviewed for possible inclusion...
March 15, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28298753/a-prospective-randomized-trial-comparing-dexmedetomidine-and-midazolam-for-conscious-sedation-during-oocyte-retrieval-in-an-in-vitro-fertilization-program
#9
Ali Mohamed Ali Elnabtity, Mohamed Fouad Selim
BACKGROUND: Various sedative and analgesic techniques have been used for pain relief during oocyte retrieval which is the most painful part of in vitro fertilization (IVF) procedures. AIM: This study aimed at comparing dexmedetomidine and midazolam for conscious sedation in women undergoing transvaginal oocyte retrieval during an IVF program. SETTINGS AND DESIGN: Prospective randomized double-blinded comparative study. PATIENTS AND METHODS: Fifty-two patients undergoing oocyte retrieval in their first IVF cycle were randomly allocated into two equal groups...
January 2017: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/28296202/dna-methylation-imprinting-errors-in-spermatogenic-cells-from-maturation-arrest-azoospermic-patients
#10
P I Marques, S Fernandes, F Carvalho, A Barros, M Sousa, C J Marques
Imprinting errors have been described in spermatozoa from infertile patients with oligozoospermia and azoospermia. However, little is known about methylation of imprinted genes in other spermatogenic cells from azoospermic patients. Therefore, we aimed to evaluate the methylation status of single CpGs located in the differentially methylated regions (DMRs) of two imprinted genes, one paternally (H19) and one maternally (MEST) methylated, in primary spermatocytes of azoospermic patients presenting complete (MAc, n = 7) and incomplete (MAi, n = 8) maturation arrest, as well as in other spermatogenic cells from MAi patients that presented focus of complete spermatogenesis in some seminiferous tubules...
March 10, 2017: Andrology
https://www.readbyqxmd.com/read/28295710/effects-of-supplementation-of-medium-with-different-antioxidants-during-in-vitro-maturation-of-bovine-oocytes-on-subsequent-embryo-production
#11
T C Sovernigo, P R Adona, P S Monzani, S Guemra, Fda Barros, F G Lopes, Clv Leal
The aim of this study was to assess the effects of different antioxidants on the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes during in vitro maturation (IVM), as well as on the production of embryos. Oocyte of slaughterhouse-derived cattle ovaries were placed in IVM with different antioxidants: quercetin (2 μM), cysteamine (100 μM), carnitine (0.5 mg/ml), vitamin C (50 μg/ml) or resveratrol (2 μM). Oocytes matured without any antioxidant supplementation were used as control...
March 10, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28295343/epithelial-membrane-protein-2-emp2-deficiency-alters-placental-angiogenesis-mimicking-features-of-human-placental-insufficiency
#12
Carmen J Williams, Alison Chu, Wendy N Jefferson, David Casero, Deepthi Sudhakar, Nevil Khurana, Claire P Hogue, Chinmayi Aryasomayajula, Priya Patel, Peggy Sullivan, Elizabeth Padilla-Banks, Shabnam Mohandessi, Carla Janzen, Madhuri Wadehra
Epithelial membrane protein-2 (EMP2) is a tetraspan protein predicted to regulate placental development. Highly expressed in secretory endometrium and trophectoderm cells, previous studies suggest that it may regulate implantation by orchestrating the surface expression of integrins and other membrane proteins. In order to test the role of EMP2 in pregnancy, mice lacking EMP2 (Emp2(-/-) ) were generated. Emp2(-/-) females are fertile but have reduced litter sizes when carrying Emp2(-/-) but not Emp2(+/-) fetuses...
March 14, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28293457/anakinra-use-during-pregnancy-report-of-a-case-with-familial-mediterranean-fever-and-infertility
#13
Ufuk İlgen, Orhan Küçükşahin
Familial Mediterranean fever (FMF), affecting people of Mediterranean origin, is an endemic and sometimes problematic disease because of colchicine resistance/intolerance, with relative lack of treatment alternatives, and disease- or treatment-related issues, such as subfertility. Anakinra, being a rational and effective treatment alternative, has no conclusive human pregnancy data. Here we report a case of FMF with infertility who became pregnant with in vitro fertilization (IVF) under treatment with anakinra, along with the pregnancy outcome...
March 2017: European Journal of Rheumatology
https://www.readbyqxmd.com/read/28292619/obesity-and-female-infertility-potential-mediators-of-obesity-s-impact
#14
REVIEW
Darcy E Broughton, Kelle H Moley
The worldwide upward trend in obesity has been dramatic, now affecting more than 20% of American women of reproductive age. Obesity is associated with many adverse maternal and fetal effects prenatally, but it also exerts a negative influence on female fertility. Obese women are more likely to have ovulatory dysfunction due to dysregulation of the hypothalamic-pituitary-ovarian axis. Women with polycystic ovarian syndrome who are also obese demonstrate a more severe metabolic and reproductive phenotype. Obese women have reduced fecundity even when eumenorrheic and demonstrate poorer outcomes with the use of in vitro fertilization...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292618/guidance-on-the-limits-to-the-number%C3%A2-of-embryos-to-transfer-a%C3%A2-committee-opinion
#15
(no author information available yet)
Based on American Society for Reproductive Medicine (ASRM) and Society for Assisted Reproductive Technology data available through 2014, ASRM's guidelines for the limits on the number of embryos to be transferred in in vitro fertilization (IVF) cycles have been further refined in continuing efforts to promote singleton gestation and reduce the number of multiple pregnancies. This version replaces the document titled Criteria for number of embryos to transfer: a committee opinion that was published most recently in August of 2013 (Fertil Steril 2013;99:44-6)...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28292612/perinatal-outcomes-in-children-born-after-fresh-or-frozen-embryo-transfer-a-catalan-cohort-study-based-on-14-262-newborns
#16
Mar Vidal, Kilian Vellvé, Mireia González-Comadran, Ana Robles, Maria Prat, Mar Torné, Ramón Carreras, Miguel A Checa
OBJECTIVE: To ascertain whether perinatal outcomes are affected by vitrification and/or controlled ovarian hyperstimulation (COH). DESIGN: Register-based cohort study. SETTING: Not applicable. PATIENT(S): Women undergoing in vitro fertilization (IVF) between 2008 and 2012 using autologous or donated eggs who had a singleton pregnancy delivered from the 24th week onward. INTERVENTION(S): Fresh embryo transfer (ET) or frozen-thawed ET in women undergoing IVF...
March 11, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28291830/soluble-cd146-an-innovative-and-non-invasive-biomarker-of-embryo-selection-for-in-vitro-fertilization
#17
Sylvie Bouvier, Odile Paulmyer-Lacroix, Nicolas Molinari, Alexandrine Bertaud, Marine Paci, Aurélie Leroyer, Stéphane Robert, Françoise Dignat George, Marcel Blot-Chabaud, Nathalie Bardin
Although progress was made in in vitro fertilization (IVF) techniques, the majority of embryos transferred fail to implant. Morphology embryo scoring is the standard procedure for most of IVF centres for choosing the best embryo, but remains limited since even the embryos classified as "top quality" may not implant. As it has been shown that i) CD146 is involved in embryo implantation and ii) membrane form is shed to generate soluble CD146 (sCD146), we propose that sCD146 in embryo supernatants may constitute a new biomarker of embryo selection...
2017: PloS One
https://www.readbyqxmd.com/read/28290521/ndrg3-gene-regulates-dsb-repair-during-meiosis-through-modulation-the-erk-signal-pathway-in-the-male-germ-cells
#18
Hongjie Pan, Xuan Zhang, Hanwei Jiang, Xiaohua Jiang, Liu Wang, Qi Qi, Yuan Bi, Jian Wang, Qinghua Shi, Runsheng Li
The N-myc downstream regulated gene (NDRG) family consists of 4 members, NDRG-1, -2, -3, -4. Physiologically, we found Ndrg3, a critical gene which led to homologous lethality in the early embryo development, regulated the male meiosis in mouse. The expression of Ndrg3 was enhanced specifically in germ cells, and reached its peak level in the pachytene stage spermatocyte. Haplo-insufficiency of Ndrg3 gene led to sub-infertility during the male early maturation. In the Ndrg3(+/-) germ cells, some meiosis events such as DSB repair and synaptonemal complex formation were impaired...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28288882/clinical-outcomes-of-patients-with-heterotopic-pregnancy-after-surgical-treatment
#19
Yiqi Guan, Caihong Ma
STUDY OBJECTIVE: This study aimed to review surgical management and clinical outcomes of patients with heterotopic pregnancy (HP) who underwent in vitro fertilization and embryo transfer (IVF-ET) and surgical treatment at the Assisted Reproductive Technology Center of Peking University Third Hospital from January 2010 to December 2015. DESIGN: We retrospectively analyzed 56 patients' general characteristics, diagnostic features, surgical management, and clinical outcomes according to medical records and follow-up telephone interviews...
March 10, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28286810/epidemiology-and-related-risk-factors-of-preterm-labor-as-an-obstetrics-emergency
#20
Ali Asghar Halimi Asl, Saeed Safari, Mohsen Parvareshi Hamrah
INTRODUCTION: Preterm birth is still a major health problem throughout the world, which results in 75% of neonatal mortality. Preterm labor not only inflicts financial and emotional distress, it may also lead to permanent disability. The present study was conducted to determine the related risk factors and preventive measures of preterm labor. METHODS: This retrospective cross-sectional study assessed all preterm labors, as well as an equal number of term labors, during seven years, at an educational hospital...
2017: Emergency (Tehran, Iran)
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