keyword
MENU ▼
Read by QxMD icon Read
search

In vitro fertilization

keyword
https://www.readbyqxmd.com/read/29458080/transcriptomic-and-phenotypic-profiling-in-developing-zebrafish-exposed-to-thyroid-hormone-receptor-agonists
#1
Derik E Haggard, Pamela D Noyes, Katrina M Waters, Robert L Tanguay
There continues to be a need to develop in vivo high-throughput screening (HTS) and computational methods to screen chemicals for interaction with the estrogen, androgen, and thyroid pathways and as complements to in vitro HTS assays. This study explored the utility of an embryonic zebrafish HTS approach to identify and classify endocrine bioactivity using phenotypically-anchored transcriptome profiling. Transcriptome analysis was conducted on zebrafish embryos exposed to 25 estrogen-, androgen-, or thyroid-active chemicals at concentrations that elicited adverse malformations or mortality at 120 hours post-fertilization in 80% of animals exposed...
February 16, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29457367/sperm-morphological-normality-under-high-magnification-is-correlated-to-male-infertility-and-predicts-embryo-development
#2
B F Zanetti, D P A F Braga, R R Provenza, R C S Figueira, A Iaconelli, E Borges
Human sperm morphology has been described as an essential parameter for the diagnosis of male infertility and a prognostic indicator of natural or assisted pregnancies. Nevertheless, standard morphological assessment remains a subjective analysis and its impact on intracytoplasmic sperm injection (ICSI) is also of limited value. The objective of this prospective cohort study was to investigate whether motile sperm organelle morphology examination (MSOME) can improve semen analysis by better defining male infertility and providing a better prognosis for ICSI up to a year later...
February 18, 2018: Andrology
https://www.readbyqxmd.com/read/29457303/long-term-effects-of-art-what-do-animals-tell-us
#3
REVIEW
V Duranthon, P Chavatte-Palmer
Early stages of mammalian embryonic development are now known to be very sensitive to their microenvironment, with long term effects on fetal, postnatal and adult health, thus extending to these early stages the concept of Developmental Origin of Health and Disease (DoHaD). In this scientific context, and with 3% of births in developed countries, safety of Assisted Reproductive Techniques procedures becomes a matter of concern. Besides, embryo technologies in domestic mammals, using huge number of embryos, do not seem to evidence heavy impacts on adult phenotypes...
February 19, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29456582/assessing-the-accuracy-of-ultrasound-estimation-of-gestational-age-during-routine-antenatal-care-in-in-vitro-fertilization-ivf-pregnancies
#4
Bridget Knight, Aaron Brereton, Roy J Powell, Helen Liversedge
Introduction: In the UK an accurate gestational age is confirmed by ultrasound measured foetal crown rump length (CRL) at 11 + 2-14 + 1 weeks of gestation. The currently recommended Robinson and Fleming crown rump length reference chart was develop in 1975. Advances in ultrasound technology and standardized crown rump length measurement training could mean this is now out of date. Our study aimed to assess its accuracy in current routine antenatal care. Methods: Retrospective data from 178 IVF pregnancies seen for routine antenatal care at a UK Regional Maternity Unit between 1 January 2006 and 1 January 2016 was retrieved...
February 2018: Ultrasound: Journal of the British Medical Ultrasound Society
https://www.readbyqxmd.com/read/29456564/role-of-granulocyte-colony-stimulating-factor-in-human-reproduction
#5
REVIEW
Maryam Eftekhar, Elham Naghshineh, Parisa Khani
As new research reveals, granulocyte colony-stimulating factor (G-CSF) plays an effective role in pregnancy success, considering that it not only affects the embryo implantation and ovarian function but also it promotes endometrial thickening and improves the pathophysiology of endometriosis, which all fundamentally lead to reducing pregnancy loss. In this review, we focus on the role of G-CSF in human reproduction. We summarized its role in ovulation, luteinized unruptured follicle syndrome, poor responders, improving repeated in vitro fertilization failure, endometrial receptivity and treatment of thin endometrium, and recurrent spontaneous abortion...
2018: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/29456560/the-effect-of-testosterone-gel-on-fertility-outcomes-in-women-with-a-poor-response-in-in-vitro-fertilization-cycles-a-pilot-randomized-clinical-trial
#6
Nasrin Saharkhiz, Shahrzad Zademodares, Saghar Salehpour, Sedighe Hosseini, Leyla Nazari, Hatav Ghasemi Tehrani
Background: In this study, the effect of testosterone gel administration during ovulation induction on the fertility rate was examined in women with a poor ovarian response in in vitro fertilization (IVF) cycles. Materials and Methods: The current study is a single-blinded, randomized clinical trial. Patients who met inclusion (Bologna) criteria were placed in the antagonist cycle group. The patients were randomly divided into two groups each included 25 participants treated with a placebo (lubricant gel, the controls) and testosterone gel (intervention)...
2018: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/29454805/mouse-preantral-follicle-growth-in-3d-co-culture-system-using-human-menstrual-blood-mesenchymal-stem-cell
#7
Zahra Rajabi, Hossein Yazdekhasti, Seyed Mohammad Hossein Noori Mugahi, Mehdi Abbasi, Somaieh Kazemnejad, Abolfazl Shirazi, Masoumeh Majidi, Amir-Hassan Zarnani
Follicle culture provides a condition which can help investigators to evaluate various aspects of ovarian follicle growth and development and impact of different components and supplementations as well as presumably application of follicle culture approach in fertility preservation procedures. Mesenchymal Stem Cells (MSCs), particularly those isolated from menstrual blood has the potential to be used as a tool for improvement of fertility. In the current study, a 3D co-culture system with mice preantral follicles and human Menstrual Blood Mesenchymal Stem Cells (MenSCs) using either collagen or alginate beads was designed to investigate whether this system allows better preantral follicles growth and development...
February 14, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29454297/intracytoplasmic-sperm-injection-icsi-of-in-vitro-matured-oocytes-with-stored-epididymal-spermatozoa-in-camel-camelus-dromedarius-effect-of-exogenous-activation-on-in-vitro-embryo-development
#8
Nisar A Wani, Seungbum Hong
Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37 °C and on ice (0-1 °C), respectively. Cumulus-oocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20-25 COCs/well) containing 500 μL of maturation medium and cultured at 38...
February 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/29453369/intratesticular-injection-followed-by-electroporation-allows-gene-transfer-in-caprine-spermatogenic-cells
#9
R Kumar Pramod, Abhijit Mitra
The production of transgenic livestock is constrained due to the limited success of currently available methods for transgenesis. Testis mediated gene transfer (TMGT) is an emerging method that shows a high success rate in generating transgenic mice. In this study, we report a newly developed protocol for electroporation-aided TMGT to produce a transgenic goat. The injectable volume and concentration of the transgene were first standardized, and then electroporation conditions were optimized in vitro. In vivo experiments were performed by injecting a transgenic construct (pIRES2-EGFP; enhanced green fluorescent protein) into the testicular interstitium followed by electroporation...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453221/h3k9-demethylase-kdm4e-is-an-epigenetic-regulator-for-bovine-embryonic-development-and-a-defective-factor-for-nuclear-reprogramming
#10
Xin Liu, Yizhi Wang, Yuanpeng Gao, Jianmin Su, Jingcheng Zhang, Xupeng Xing, Chuan Zhou, Kezhen Yao, Quanli An, Yong Zhang
Aberrant epigenetic reprogramming often results in developmental defects in somatic cell nuclear transfer (SCNT) embryos during embryonic genome activation (EGA). Bovine eight-cell SCNT embryos exhibit global hypermethylation of histone H3 lysine 9 tri- and di-methylation (H3K9me3/2), but the intrinsic reason for this remains elusive. Here, we provide evidence that two H3K9 demethylase genes, lysine-specific demethylase 4D ( KDM4D ) and 4E ( KDM4E ), are related to active H3K9me3/2 demethylation in in vitro fertilized (IVF) embryos and are deficiently expressed in cloned embryos at the time of EGA...
February 16, 2018: Development
https://www.readbyqxmd.com/read/29453138/increased-coagulation-index-as-measured-by-thromboelastography-during-ovarian-stimulation-for-in-vitro-fertilization-influence-of-the-final-oocyte-maturation-triggering-agent
#11
Ronit Beck-Fruchter, Israel Gavish, Shira Baram, Yoel Geslevich, Amir Weiss
OBJECTIVE: Thromboelastography (TEG) is a viscoelastic test of hemostasis which allows measurement of the processes of clot initiation, propagation, stabilization, and dissolution in real time. In this study we aimed to evaluate the alterations in coagulation as measured by TEG during In Vitro Fertilization (IVF) stimulation cycles and to investigate whether final oocyte maturation with recombinant hCG (rhCG) versus GnRH agonist results in a different coagulation state. STUDY DESIGN: This is a prospective observational study which included fifty-three normogonadotrophic women...
February 10, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29452330/atypical-teratoid-rhabdoid-tumor-after-in-vitro-fertilization-illustrative-case-report-and-systematic-literature-review
#12
REVIEW
Adomas Bunevicius, Algimantas Matukevicius, Vytenis Deltuva, Inga Gudinaviciene, Darius Pranys, Arimantas Tamsauskas
OBJECTIVE: In vitro fertilization (IVF) is increasingly used for treatment of infertile couples worldwide. The association between IVF and cancer risk in offsprings is conflicting. We present a case of atypical teratoid/rhabdoid tumor (AT/RT) in a girl conceived by IVF and present results of systematic review of literature of primary intracranial neoplasms diagnosed in children conceived by IVF. METHODS: A systematic review of literature was conducted on April 12, 2017 to identify previously published reports of intracranial brain tumors in patients conceived after IVF...
February 13, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29449939/innovative-approaches-to-genome-editing-in-avian-species
#13
REVIEW
Caitlin A Cooper, Timothy J Doran, Arjun Challagulla, Mark L V Tizard, Kristie A Jenkins
The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has come advancements in technologies used to deliver them. In mammals genome engineering tools are typically delivered into in vitro fertilized single cell embryos which are subsequently cultured and then implanted into a recipient animal. In avian species this is not possible, so other methods have been developed for genome engineering in birds...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29449037/impact-of-light-irradiation-on-preservation-and-function-of-mammalian-spermatozoa
#14
REVIEW
Marc Yeste, Miriam Castillo-Martín, Sergi Bonet, Joan E Rodríguez-Gil
Light irradiation has been demonstrated to exert positive effects on gametes, and particularly on sperm. In effect, a high number of studies conducted in several species, including humans, mice, pigs, cattle and sheep, and using different light sources (such as lasers and light-emitting diodes) have demonstrated that photo-stimulation increases sperm motility. In addition, other works have shown that sperm fertilizing ability both in vitro and in vivo can be increased following light irradiation; there are also some evidences pointing out to an extend of lifespan of preserved semen...
February 9, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29448847/effect-of-early-cumulus-cells-removal-and-early-rescue-icsi-on-pregnancy-outcomes-in-high-risk-patients-of-fertilization-failure
#15
Yuxia He, Haiying Liu, Haiyan Zheng, Li Li, Xin Fu, Jianqiao Liu
OBJECTIVE: The objective of this retrospective study was to evaluate clinical outcomes of early cumulus cells removal and early rescue intracytoplasmic sperm injection (ICSI) in high-risk patients of fertilization failure during human in vitro fertilization (IVF). METHODS: A total of 5,518 patients were enrolled between January 2014 to December 2016. Of which 505 couples performed short insemination with >30% fertilization rate were included in short-term IVF group, 102 patients detected total fertilization failure (TFF) were treated with early rescue ICSI (R-ICSI group), and 4911 couples underwent conventional IVF with overnight co-incubation of gametes (traditional IVF group)...
February 15, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29448196/bisphenol-s-induces-obesogenic-effects-through-deregulating-lipid-metabolism-in-zebrafish-danio-rerio-larvae
#16
Weiwei Wang, Xiaona Zhang, Zihao Wang, Jingyu Qin, Wei Wang, Hua Tian, Shaoguo Ru
It has been suggested that dramatic increase in obesity may be caused by growing exposure to environmental chemicals. In vitro data has suggested bisphenol S (BPS), a compound widely used in polycarbonate plastic production, can induce lipid accumulation in preadipocytes. However, the mechanisms responsible for BPS-induced obesity in vivo remain unclear. In this study, we used translucent zebrafish (Danio rerio) larvae as a model to investigate the effect of environmentally relevant BPS exposure (1, 10, and 100 μg/L from 2 h to 15 d post fertilization) on lipid accumulation, triacylglycerol (TAG) and lipoproteins content, and mRNA expression of genes involved in the regulation of lipid synthesis, transport, degradation, and storage...
February 3, 2018: Chemosphere
https://www.readbyqxmd.com/read/29447664/prevention-of-in-vitro-fertilization-twins-should-focus-on-maximizing-single-embryo-transfer-versus-twins-are-an-acceptable-complication-of-in-vitro-fertilization
#17
EDITORIAL
David R Meldrum, Eli Y Adashi, V Gabriel Garzo, Norbert Gleicher, Jean Parinaud, Anja Pinborg, Brad Van Voorhis
No abstract text is available yet for this article.
February 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29447380/sirtuins-in-gamete-biology-and-reproductive-physiology-emerging-roles-and-therapeutic-potential-in-female-and-male-infertility
#18
Carla Tatone, Giovanna Di Emidio, Arcangelo Barbonetti, Gaspare Carta, Alberto M Luciano, Stefano Falone, Fernanda Amicarelli
BACKGROUND: Sirtuins (SIRT1-7) are a family of NAD+-dependent deacetylases that catalyze post-translational modifications of proteins. Together, they respond to metabolic challenges, inflammatory signals or hypoxic/oxidative stress, and are associated with aging and longevity. The role of Sirtuins in the regulation of fertility emerged in 2003 when a defective reproductive phenotype was observed in SIRT1-null mice. Although studies on Sirtuins in reproductive biology have been increasing in the last years, a recent comprehensive update on this issue is still lacking...
February 13, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29447173/dynamic-changes-of-setd2-a-histone-h3k36-methyltransferase-in-porcine-oocytes-ivf-and-scnt-embryos
#19
Yun Fei Diao, Tao Lin, Xiaoxia Li, Reza K Oqani, Jae Eun Lee, So Yeon Kim, Dong Il Jin
SETD2 (SET domain containing protein 2) acts as a histone H3 lysine 36 (H3K36)-specific methyltransferase and may play important roles in active gene transcription in human cells. However, its expression and role in porcine oocytes and preimplantation embryos are not well understood. Here, we used immunofluorescence and laser scanning confocal microscopy to examine SETD2 expression in porcine fetal fibroblasts, oocytes, and preimplantation embryos derived from in vitro fertilization (IVF), parthenogenetic activation (PA), and somatic cell nuclear transfer (SCNT)...
2018: PloS One
https://www.readbyqxmd.com/read/29447147/assisted-reproductive-technology-surveillance-united-states-2015
#20
Saswati Sunderam, Dmitry M Kissin, Sara B Crawford, Suzanne G Folger, Sheree L Boulet, Lee Warner, Wanda D Barfield
PROBLEM/CONDITION: Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Although the majority of infants conceived through ART are singletons, women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants...
February 16, 2018: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
keyword
keyword
33520
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"