keyword
MENU ▼
Read by QxMD icon Read
search

Stem cell

keyword
https://www.readbyqxmd.com/read/29929189/dexmedetomidine-protects-neural-stem-cells-from-ketamine-induced-injury
#1
Pan Lu, Shan Lei, Weisong Li, Yang Lu, Juan Zheng, Ning Wang, Yongjun Xia, Haixia Lu, Xinlin Chen, Yong Liu, Peng-Bo Zhang
BACKGROUND/AIMS: Ketamine inhibits the proliferation of neural stem cells (NSCs) and disturbs normal neurogenesis. Dexmedetomidine provides neuroprotection against volatile anesthetic-induced neuroapoptosis and cognitive impairment in the developing brain. Whether it may protect NSCs from ketamine-induced injury remains unknown. In this study, we investigated the protective effects of dexmedetomidine on ketamine-exposed NSCs and explored the mechanisms potentially involved. METHODS: Primary NSC cultures were characterized using immunofluorescence...
June 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29928948/single-dose-daily-fractionated-is-not-inferior-to-twice-a-day-fractionated-total-body-irradiation-prior-to-allogeneic-stem-cell-transplantation-for-acute-leukemia-a-useful-practice-simplification-resulting-from-the-sarasin-study
#2
Yazid Belkacemi, Myriam Labopin, Sebastian Giebel, Norbert Claude Gorin, Gokoulakrichenane Loganadane, Leszek Miszczyk, Mauricette Michallet, Gerard Socié, Nicolaas Pm Schaap, Jan J Cornelissen, Ibrahim Yakoub-Agha, Emmanuelle Polge, Mohamad Mohty, Arnon Nagler
PURPOSE: Total-body irradiation (TBI) is a major constituent of myeloablative conditioning regimens. The standard technique consists of 12Gy in 6 fractions over 3 days. The SARASIN study aimed to compare standard-fractionation to one-daily fractionation prior to transplant in leukemia. MATERIAL AND METHODS: We retrospectively compared TBI regimens delivered in 2993 patients from the EBMT database, transplanted between 2000 and 2014 for acute lymphoblastic (ALL, n=1729) or acute myelogenous (AML, n=1264) leukemia...
June 18, 2018: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/29928900/characterization-and-comparison-of-human-limbal-explant-cultures-grown-under-defined-and-xeno-free-conditions
#3
Kristyna Brejchova, Peter Trosan, Pavel Studeny, Pavlina Skalicka, Tor Paaske Utheim, Jan Bednar, Katerina Jirsova
Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative and qualitative lot-to-lot variations. Human limbal explants were plated on untreated or fibrin coated plastic plates and cultured in two non-xenogeneic media (supplemented with either human serum or platelet lysate only)...
June 18, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29928848/retrospective-evaluation-of-fidaxomicin-versus-oral-vancomycin-for-treatment-of-clostridium-difficile-infections-in-allogeneic-stem-cell-transplant
#4
Laura Prohaska, Zahra Mahmoudjafari, Leyla Shune, Anurag Singh, Tara Lin, Sunil Abhyankar, Siddhartha Ganguly, Dennis Grauer, Joseph McGuirk, Lisa Clough
OBJECTIVE/BACKGROUND: Clostridium difficile infection (CDI) is a potential complication during hematopoietic stem cell transplantation (HSCT), and no specific recommendations exist regarding treatment of CDI in allogeneic SCT patients. Use of metronidazole and oral vancomycin has been associated with clinical failure. Fidaxomicin has previously been found noninferior to the use of oral vancomycin for the treatment of CDI, and no studies have compared the use of oral vancomycin with fidaxomicin for the treatment of CDI in allogeneic SCT...
June 18, 2018: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/29928682/integration-of-sensors-in-gastrointestinal-organoid-culture-for-biological-analysis
#5
REVIEW
Ge-Ah Kim, Nicholas J Ginga, Shuichi Takayama
The gastrointestinal (GI) tract regulates physiologic responses in complex ways beyond facilitating nutrient entry into the circulatory system. Because of the anatomic location of the GI tract, studying in vivo physiology of the human gut, including host cell interaction with the microbiota, is limited. GI organoids derived from human stem cells are gaining interest as they recapitulate in vivo cellular phenotypes and functions. An underdeveloped capability that would further enhance the utility of these miniature models of the GI tract is to use sensors to quantitatively characterize the organoid systems with high spatiotemporal resolution...
2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29928681/decrypting-the-crypt-novel-monoclonal-antibodies-to-identify-intestinal-stem-cell-populations
#6
EDITORIAL
Breanna Sheahan, Christopher M Dekaney
No abstract text is available yet for this article.
2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29928673/monoclonal-antibodies-reveal-dynamic-plasticity-between-lgr5-and-bmi1-expressing-intestinal-cell-populations
#7
Nicholas R Smith, John R Swain, Paige S Davies, Alexandra C Gallagher, Michael S Parappilly, Catherine Z Beach, Philip R Streeter, Ian A Williamson, Scott T Magness, Melissa H Wong
Background & Aims: Continual renewal of the intestinal epithelium is dependent on active- and slow-cycling stem cells that are confined to the crypt base. Tight regulation of these stem cell populations maintains homeostasis by balancing proliferation and differentiation to support critical intestinal functions. The hierarchical relation of discrete stem cell populations in homeostasis or during regenerative epithelial repair remains controversial. Although recent studies have supported a model for the active-cycling leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)+ intestinal stem cell (ISC) functioning upstream of the slow-cycling B lymphoma Mo-MLV insertion region 1 homolog (Bmi1)-expressing cell, other studies have reported the opposite relation...
2018: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/29928656/long-term-phase-1-2-intraspinal-stem-cell-transplantation-outcomes-in-als
#8
Stephen A Goutman, Morton B Brown, Jonathan D Glass, Nicholas M Boulis, Karl Johe, Tom Hazel, Merit Cudkowicz, Nazem Atassi, Lawrence Borges, Parag G Patil, Stacey A Sakowski, Eva L Feldman
Objective: Intraspinal human spinal cord-derived neural stem cell (HSSC) transplantation is a potential therapy for amyotrophic lateral sclerosis (ALS); however, previous trials lack controls. This post hoc analysis compared ambulatory limb-onset ALS participants in Phase 1 and 2 (Ph1/2) open-label intraspinal HSSC transplantation studies up to 3 years after transplant to matched participants in Pooled Resource Open-Access ALS Clinical Trials (PRO-ACT) and ceftriaxone datasets to provide required analyses to inform future clinical trial designs...
June 2018: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/29928592/complex-toxicity-as-disruption-of-adipocyte-or-osteoblast-differentiation-in-human-mesenchymal-stem-cells-under-the-mixed-condition-of-tbbpa-and-tcdd
#9
Hideki Kakutani, Tomohiro Yuzuriha, Ema Akiyama, Teruyuki Nakao, Souichi Ohta
People are frequently and unintentionally exposed to many chemical compounds, such as environmental pollutants and endocrine-disrupting chemicals (EDCs), in food and from the atmosphere. In particular, endocrine-disrupting TBBPA and dioxins are found in human breast milk and in the body. Conventional studies evaluate toxicity by administering a single substance to cells or animals, but evaluation of the toxicity of mixtures of these ingested compounds is essential for "true" toxicological assessment...
2018: Toxicology Reports
https://www.readbyqxmd.com/read/29928510/monolayer-culture-of-intestinal-epithelium-sustains-lgr5-intestinal-stem-cells
#10
Yuan Liu, Zhen Qi, Xintong Li, Yanan Du, Ye-Guang Chen
No abstract text is available yet for this article.
2018: Cell Discovery
https://www.readbyqxmd.com/read/29928490/metabolite-profiling-identifies-a-signature-of-tumorigenicity-in-hepatocellular-carcinoma
#11
Shamir Cassim, Valérie-Ann Raymond, Benoit Lacoste, Pascal Lapierre, Marc Bilodeau
HCC (Hepatocellular carcinoma) cells exhibit greater metabolic plasticity than normal hepatocytes since they must survive in a dynamic microenvironment where nutrients and oxygen are often scarce. Using a metabolomic approach combined with functional in vitro and in vivo assays, we aimed to identify an HCC metabolic signature associated with increased tumorigenicity and patient mortality. Metabolite profiling of HCC Dt81Hepa1-6 cells revealed that their increased tumorigenicity was associated with elevated levels of glycolytic metabolites...
June 1, 2018: Oncotarget
https://www.readbyqxmd.com/read/29928478/an-nf%C3%AE%C2%BAb-dependent-mechanism-of-tumor-cell-plasticity-and-lateral-transmission-of-aggressive-features
#12
Nancy Adriana Espinoza-Sánchez, Jennifer Enciso, Rosana Pelayo, Ezequiel M Fuentes-Pananá
Breast cancer is a complex disease exhibiting extensive inter- and intra-tumor heterogeneity. Inflammation is a well-known driver of cancer progression, often attributed to immune cells infiltrating the tumor stroma. However, tumor cells themselves are capable to secrete a variety of inflammatory molecules, of which we understand very little about their role in intra-clonal communication. We recently reported the capacity of triple negative cell lines to induce a cancer stem cell (CSC)-like phenotype and invasion properties into luminal cells, a mechanism mediated by pro-inflammatory cytokines that up-regulated the CXCL12/CXCR4/CXCR7 chemokine signaling axis...
June 1, 2018: Oncotarget
https://www.readbyqxmd.com/read/29928453/effects-of-the-novel-polyphenol-conjugate-dpp-23-on-head-and-neck-squamous-cell-carcinoma-cells-in-vitro
#13
Johannes Taeger, Agmal Scherzad, Doris Feineis, Raina Seupel, Rudolf Hagen, Norbert Kleinsasser, Stephan Hackenberg
Despite partial advances in therapy for patients suffering from head and neck squamous cell carcinomas (HNSCC), prognosis still remains poor with minimal improvement in survival for over the last several decades. Some agents found are known to cause cancer cell death in vitro by promoting cellular reactive oxygen species (ROS) accumulation. This is particularly of interest as some cancer cells are more sensitive to ROS than normal cells. It could be shown that the novel polyphenol conjugate (E)-3-(3',5'-Dimethoxyphenyl)-1-(2'-methoxyphenyl)prop-2-en-1-one (DPP-23) offers antitumor effects by the selective generation of ROS without an indication of toxicity in normal tissues in vitro and in vivo ...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928431/p53-positively-regulates-the-expression-of-cancer-stem-cell-marker-cd133-in-hct116-colon-cancer-cells
#14
Xia Chen, Hua Guan, Xiao-Dan Liu, Da-Fei Xie, Yu Wang, Teng Ma, Bo Huang, Ping-Kun Zhou
Colon cancer stem cells (CSCs), which are highly capable of self-renewal and proliferation, are involved in colon tumorigenesis and response to therapy. CD133 is considered the most robust surface marker for colorectal cancer stem cells. Although the TP53 gene is frequently mutated in colon cancer, it remains not fully understood whether and how tumor protein p53 (p53) is associated with CD133 expression in colon cancer cells. In the present study, the expression of the CSC biomarker CD133 was investigated in terms of p53 status in colorectal carcinoma HCT116 cells...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928419/comparative-proteomics-of-side-population-cells-derived-from-human-hepatocellular-carcinoma-cell-lines-with-varying-metastatic-potentials
#15
Hongzhi Liu, Yingchao Wang, Xiaohua Xing, Ying Sun, Dahai Wei, Geng Chen, Qinying Liu, Shanshan Chen, Xiaolong Liu, Jingfeng Liu
Metastasis and recurrence following surgery are major reasons for the high mortality rate and poor prognosis associated with hepatocellular carcinoma (HCC). Cancer stem cells (CSCs) are thought to be able to cause cancer, and to be the primary cause of tumor recurrence and metastasis. The underlying mechanisms of the metastatic potential of CSCs is poorly understood. In the present study, side population (SP) cells were isolated from 4 HCC cell lines, and their self-renewal and migratory abilities were compared...
July 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928373/mesenchymal-stem-cells-confer-resistance-to-doxorubicin-induced-cardiac-senescence-by-inhibiting-microrna-34a
#16
Wenzheng Xia, Meng Hou
Doxorubicin (DOXO) is a chemotherapeutic agent widely used in the treatment of various types of cancer. However, cardiotoxicity is a major side effect of DOXO therapy due to the ability of this compound to induce cardiac cellular senescence. It is well known that microRNA (miR)-34a serves a role in cardiac dysfunction and ageing, and that it is involved in several cellular processes associated with DOXO-induced cardiotoxicity. Furthermore, mesenchymal stem cell (MSC)-based therapies have been reported to modulate cellular senescence...
June 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928366/s100a16-up-regulates-oct4-and-nanog-expression-in-cancer-stem-like-cells-of-yumoto-human-cervical-carcinoma-cells
#17
Nariaki Tomiyama, Ryuji Ikeda, Yukihiko Nishizawa, Shogo Masuda, Yusuke Tajitsu, Yasuo Takeda
Cancer stem-like cells (CSCs), which possess the ability to self-renewal and are multipotent, are regarded as the cause of tumor formation, recurrence, metastasis and drug resistance. It is necessary to understand the properties of CSCs in order to treat them effectively. It has been previously reported that S100 family proteins, which carry calcium-binding EF-hand motifs and are associated with tumorigenic processes, serve crucial roles in maintaining cancer stem-like properties. S100A16 is upregulated in various types of cancer, including bladder, lung and pancreatic...
June 2018: Oncology Letters
https://www.readbyqxmd.com/read/29928131/identification-of-cd24-as-a-marker-for-tumorigenesis-of-melanoma
#18
Ming-Rui Tang, Jia-Yan Guo, Di Wang, Nan Xu
Objective: Cutaneous melanoma (CM) is a common skin cancer. Surgery is still the primary treatment for CM, as melanoma is resistant to chemotherapy. In the recent years, it has been found that cancer stem-like cells (CSCs) are responsible for this drug resistance. CD24 is a widely used marker to isolate CSCs. In this study, we aimed to analyze the properties of CD24+ and CD24- subpopulation of melanoma cells. Materials and methods: We isolated CD24+ cells CSCs using magnetic-activated cell sorting system...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29928084/autologous-emulsified-fat-injection-for-rejuvenation-of-scars-a-prospective-observational-study
#19
Lekshmi S Bhooshan, M Geetha Devi, R Aniraj, P Binod, M Lekshmi
Background: The skin rejuvenation potential of the autologous emulsified nanofat was studied by Tonnard et al . in 2013. This property is due to the viable adipose-derived stem cells present in the nanofat; although, there are no viable adipocytes. The aim of this study was to determine the aesthetic outcome of autologous emulsified nanofat injection in scars using a standardised and validated Patient Observer Scar Assessment Scale (POSAS) and photographs. Materials and Methods: A total of 34 patients with scars of varied aetiologies were included in the study as per inclusion criteria...
January 2018: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/29928062/novel-antibody-reagents-for-characterization-of-drug-and-tumor-microenvironment-induced-changes-in-epithelial-mesenchymal-transition-and-cancer-stem-cells
#20
Tony Navas, Thomas D Pfister, Simona Colantonio, Amina Aziz, Lynda Dieckman, Richard G Saul, Jan Kaczmarczyk, Suzanne Borgel, Sergio Y Alcoser, Melinda G Hollingshead, Young H Lee, Donald P Bottaro, Tara Hiltke, Gordon Whiteley, Naoko Takebe, Robert J Kinders, Ralph E Parchment, Joseph E Tomaszewski, James H Doroshow
The presence of cancer stem cells (CSCs) and the induction of epithelial-to-mesenchymal transition (EMT) in tumors are associated with tumor aggressiveness, metastasis, drug resistance, and poor prognosis, necessitating the development of reagents for unambiguous detection of CSC- and EMT-associated proteins in tumor specimens. To this end, we generated novel antibodies to EMT- and CSC-associated proteins, including Goosecoid, Sox9, Slug, Snail, and CD133. Importantly, unlike several widely used antibodies to CD133, the anti-CD133 antibodies we generated recognize epitopes distal to known glycosylation sites, enabling analyses that are not confounded by differences in CD133 glycosylation...
2018: PloS One
keyword
keyword
335
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"