keyword
MENU ▼
Read by QxMD icon Read
search

Ex-vivo rbc

keyword
https://www.readbyqxmd.com/read/29630135/magnesium-bioavailability-after-administration-of-sucrosomial%C3%A2-magnesium-results-of-an-ex-vivo-study-and-a-comparative-double-blinded-cross-over-study-in-healthy-subjects
#1
E Brilli, S Khadge, A Fabiano, Y Zambito, T Williams, G Tarantino
OBJECTIVE: We conducted an ex-vivo analysis and a study in healthy subjects to compare magnesium bioavailability after administration of Sucrosomial® magnesium or commercially available preparations of magnesium citrate, magnesium oxide and magnesium bisglycinate. MATERIALS AND METHODS: In the ex-vivo study we simulated magnesium intestinal absorption after digestion through sections of intestinal mucosa isolated from rats. We compared the absorption of magnesium oxide and Sucrosomial® magnesium at two different concentrations: 32...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29614639/microparticles-in-sickle-cell-disease
#2
Marc Romana, Philippe Connes, Nigel S Key
Several pathophysiological pathways in sickle cell disease (SCD), the most prevalent hemoglobinopathy worldwide, result in activation of circulating blood cells and the release of submicron vesicles, so-called microparticles (MPs). MPs are candidate biomarkers in vascular disease that exhibit functional biological properties. Compared to healthy individuals, higher level of plasma MPs, mostly derived from platelets and red blood cells (RBC), has been repeatedly observed in SCD patients in their steady-state condition...
2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29611201/a-practical-toxicity-bioassay-for-vicine-convicine-levels-in-faba-bean-vicia-faba
#3
Fitsum Getachew, Albert Vandenberg, Judit Smits
BACKGROUND: Faba bean (Vicia faba) vicine/convicine (V-C) aglycones (divicine/isouramil) provoke an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) enzyme defect in their red blood cells. Geneticists/plant breeders are working with faba bean to decrease V-C levels to improve public acceptance of this high protein pulse crop. Here, we present a fast and simple ex-vivo in vitro bioassay for V-C toxicity testing of faba bean or faba bean food products...
April 2, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29580430/hydroxyethyl-methacrylate-grafted-carboxy-methyl-tamarind-cmt-g-hema-polysaccharide-based-matrix-as-a-suitable-scaffold-for-skin-tissue-engineering
#4
Priyanka Choudhury, Satish Kumar, Abhishek Singh, Ashutosh Kumar, Navneet Kaur, Sridhar Sanyasi, Saurabh Chawla, Chandan Goswami, Luna Goswami
Patho-physiologies related to skin are diverse in nature such as burns, skin ulcers, atopic dermatitis, psoriasis etc. which impose severe bio-medical problems and thus enforce requirement of new and healthy skin prepared through tissues engineering methodologies. However, fully functional and biodegradable matrix for attachment, growth, proliferation and differentiation of the relevant cells is not available. In the present study, we introduce a set of hydrogels synthesized by incorporation of a synthetic monomer (Hydroxyethlmethacryate) with a semi-synthetic polymer backbone (carboxy methyl tamarind, CMT) in different mole ratios...
June 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29576709/antioxidant-and-hemolysis-protective-effects-of-polyphenol-rich-extract-from-mulberry-fruits
#5
Palanigounder Ganeshan Ajay Krishna, Thasma Raman Sivakumar, Chao Jin, Shao-Hu Li, Yu-Jie Weng, Juan Yin, Jun-Qiang Jia, Chu-Yan Wang, Zhong-Zheng Gui
Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti-inflammatory, and chemoprotective properties. Objectives: In this study, purification of the polyphenol-rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro ...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29572498/a-single-high-fat-meal-provokes-pathological-erythrocyte-remodeling-and-increases-myeloperoxidase-levels-implications-for-acute-coronary-syndrome
#6
Tyler W Benson, Neal L Weintraub, Ha Won Kim, Nichole Seigler, Sanjiv Kumar, Jonathan Pye, Tetsuo Horimatsu, Rod Pellenberg, David W Stepp, Rudolf Lucas, Vladimir Y Bogdanov, Sheldon E Litwin, Julia E Brittain, Ryan A Harris
High-fat meal (HFM) consumption can produce acute lipemia and trigger myocardial infarction in patients with atherosclerosis, but the mechanisms are poorly understood. Erythrocytes (red blood cells, RBCs) intimately interact with inflammatory cells and blood vessels and play a complex role in regulating vascular function. Chronic high-fat feeding in mice induces pathological RBC remodeling, suggesting a novel link between HFM, RBCs, and vascular dysfunction. However, whether acute HFM can induce RBC remodeling in humans is unknown...
March 23, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29567949/ocimum-flavone-orientin-as-a-countermeasure-for-thrombocytopenia
#7
Marshleen Yadav, Feifei Song, Jason Huang, Arnab Chakravarti, Naduparambil K Jacob
Thrombocytopenia or chronic depletion of platelets in blood, could create life-threatening conditions in patients who receive aggressive systemic radiation and chemotherapy. Currently there are no approved agents for the rapid treatment of thrombocytopenia. In the present study, we demonstrate that administration of Orientin, a glycosidic flavonoid or dietary administration of Orientin containing Tulsi (Holy Basil) leaves, results in a significant increase in circulating platelets in a clinically relevant mouse model...
March 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29443412/an-ex-vivo-comparison-of-vascular-access-devices-used-in-extracorporeal-photopheresis
#8
Jeffrey Szymanski, Priyank Shah, Marian Dynis, Donna Mansfield, Connie Hamilton, George Despotis
BACKGROUND: Central venous access devices are commonly used in extracorporeal photopheresis, but their performance has not been systematically evaluated. The primary objective of this study was to compare pressures at various flow rates for central venous access devices in an ex vivo simulation of photopheresis. STUDY DESIGN AND METHODS: Diluted, heparinized red blood cells were circulated through central access devices in series with a photopheresis system, and pressures at several flow rates were recorded...
February 2018: Transfusion
https://www.readbyqxmd.com/read/29432581/14q32-and-let-7-micrornas-regulate-transcriptional-networks-in-fetal-and-adult-human-erythroblasts
#9
Samuel Lessard, Mélissa Beaudoin, Stuart H Orkin, Daniel E Bauer, Guillaume Lettre
In humans, fetal erythropoiesis takes place in the liver whereas adult erythropoiesis occurs in the bone marrow. Fetal and adult erythroid cells are not only produced at different sites, but are also distinguished by their respective transcriptional program. In particular, whereas fetal erythroid cells express γ-globin chains to produce fetal hemoglobin (HbF), adult cells express β-globin chains to generate adult hemoglobin. Understanding the transcriptional regulation of the fetal-to-adult hemoglobin switch is clinically important as re-activation of HbF production in adult erythroid cells would represent a promising therapy for the hemoglobin disorders sickle cell disease and β-thalassemia...
February 8, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29421563/lipophilic-indocarbocyanine-conjugates-for-efficient-incorporation-of-enzymes-antibodies-and-small-molecules-into-biological-membranes
#10
Weston J Smith, Huy Tran, James I Griffin, Jessica Jones, Vivian P Vu, Lizanne Nilewski, Nathan Gianneschi, Dmitri Simberg
Decoration of cell membranes with biomolecules, targeting ligands and imaging agents is an emerging strategy to improve functionality of cell-based therapies. Compared to covalent chemistry or genetic expression on the cell surface, lipid painting (i.e., incorporation of lipid-conjugated molecules into the cell bilayer) is a fast, non-damaging and less expensive approach. Previous studies demonstrated excellent incorporation and retention of distearyl indocarbocyanine dye DiI in membranes of cells in vitro and in vivo...
February 2, 2018: Biomaterials
https://www.readbyqxmd.com/read/29280988/induced-pluripotent-stem-cell-derived-red-blood-cells-and-platelet-concentrates-from-bench-to-bedside
#11
REVIEW
Daniele Focosi, Giovanni Amabile
Red blood cells and platelets are anucleate blood components indispensable for oxygen delivery and hemostasis, respectively. Derivation of these blood elements from induced pluripotent stem (iPS) cells has the potential to develop blood donor-independent and genetic manipulation-prone products to complement or replace current transfusion banking, also minimizing the risk of alloimmunization. While the production of erythrocytes from iPS cells has challenges to overcome, such as differentiation into adult-type phenotype that functions properly after transfusion, platelet products are qualitatively and quantitatively approaching a clinically-applicable level owing to advances in expandable megakaryocyte (MK) lines, platelet-producing bioreactors, and novel reagents...
December 27, 2017: Cells
https://www.readbyqxmd.com/read/29167669/iron-induces-anti-tumor-activity-in-tumor-associated-macrophages
#12
Milene Costa da Silva, Michael O Breckwoldt, Francesca Vinchi, Margareta P Correia, Ana Stojanovic, Carl Maximilian Thielmann, Michael Meister, Thomas Muley, Arne Warth, Michael Platten, Matthias W Hentze, Adelheid Cerwenka, Martina U Muckenthaler
Tumor-associated macrophages (TAMs) frequently help to sustain tumor growth and mediate immune suppression in the tumor microenvironment (TME). Here, we identified a subset of iron-loaded, pro-inflammatory TAMs localized in hemorrhagic areas of the TME. The occurrence of iron-loaded TAMs (iTAMs) correlated with reduced tumor size in patients with non-small cell lung cancer. Ex vivo experiments established that TAMs exposed to hemolytic red blood cells (RBCs) were converted into pro-inflammatory macrophages capable of directly killing tumor cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29122658/endothelium-derived-contraction-in-a-model-of-rheumatoid-arthritis-is-mediated-via-angiotensin-ii-type-1-receptors
#13
Kayleigh Hamilton, Lynette Dunning, William R Ferrell, John C Lockhart, Andrew MacKenzie
A role for endothelium-derived constricting factors (EDCF), and the angiotensin II type 1 receptor (AT1R) pathway, in the vascular impairment found in the rat Freund's complete adjuvant (FCA)-model of rheumatoid arthritis (RA) was examined. FCA arthritis was induced in rats±losartan. Vehicle-treated rats served as controls. Knee-joint swelling and red blood cell (RBC) aggregation were measured as indicators of inflammation and endothelium reactivity assessed by response to acetylcholine (ACh) on aortic rings...
January 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29111410/the-z-spectrum-from-human-blood-at-7t
#14
Simon M Shah, Olivier E Mougin, Andrew J Carradus, Nicolas Geades, Richard Dury, William Morley, Penny A Gowland
Chemical Exchange Saturation Transfer (CEST) has been used to assess healthy and pathological tissue in both animals and humans. However, the CEST signal from blood has not been fully assessed. This paper presents the CEST and nuclear Overhauser enhancement (NOE) signals detected in human blood measured via z-spectrum analysis. We assessed the effects of blood oxygenation levels, haematocrit, cell structure and pH upon the z-spectrum in ex vivo human blood for different saturation powers at 7T. The data were analysed using Lorentzian difference (LD) model fitting and AREX (to compensate for changes in T1 ), which have been successfully used to study CEST effects in vivo...
October 27, 2017: NeuroImage
https://www.readbyqxmd.com/read/29107162/artificial-stroke-clots-how-wide-is-the-gap-to-the-real-world
#15
Maria Berndt, Sascha Prothmann, Christian Maegerlein, Paul Oberdieck, Claus Zimmer, Barbara Hegge, Jaroslav Pelisek, Lucas Schirmer, Holger Poppert, Tobias Boeckh-Behrens
OBJECTIVE: Especially since the establishment of mechanical thrombectomy as part of standard stroke therapy, artificial thrombi have become important in the training of interventionalists as well as for the development and testing of devices. So far, these in vitro clots have lacked direct comparisons with ex vivo thrombi. We therefore compared the histologic appearance of dynamically produced clots with that of stroke thrombi acquired during mechanical recanalization. METHODS: Thrombi of 145 consecutive patients with stroke with large-vessel occlusions were histologically compared with 10 artificial clots, dynamically created from human blood and pig's blood using a Chandler loop system...
February 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29103845/negative-pressure-ventilation-decreases-inflammation-and-lung-edema-during-normothermic-ex-vivo-lung-perfusion
#16
Nader S Aboelnazar, Sayed Himmat, Sanaz Hatami, Christopher W White, Mohamad S Burhani, Peter Dromparis, Nobutoshi Matsumura, Ganghong Tian, Jason R B Dyck, Michael Mengel, Darren H Freed, Jayan Nagendran
BACKGROUND: Normothermic ex-vivo lung perfusion (EVLP) using positive pressure ventilation (PPV) and both acellular and red blood cell (RBC)-based perfusate solutions have increased the rate of donor organ utilization. We sought to determine whether a negative pressure ventilation (NPV) strategy would improve donor lung assessment during EVLP. METHODS: Thirty-two pig lungs were perfused ex vivo for 12 hours in a normothermic state, and were allocated equally to 4 groups according to the mode of ventilation (positive pressure ventilation [PPV] vs NPV) and perfusate composition (acellular vs RBC)...
April 2018: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/29101126/induction-of-deubiquitinating-enzyme-usp50-during-erythropoiesis-and-its-potential-role-in-the-regulation-of-ku70-stability
#17
Junting Cai, Jianxin Wei, Valerie Schrott, Jing Zhao, Grant Bullock, Yutong Zhao
Anemia is a very common blood disorder that affects the lives of billions of people worldwide. Anemia is caused by the loss of blood, increased destruction of red blood cells (RBCs), or reduced production of RBCs. Erythropoiesis is the complex process of RBC differentiation and maturation, in which protein degradation plays a crucial role. Protein ubiquitination regulates programmed protein degradation, which can be reversed by deubiquitinating enzymes (DUBs); however, the role of DUBs in erythropoiesis has not been well studied...
January 2018: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/29074889/unravelling-pathways-downstream-sox6-induction-in-k562-erythroid-cells-by-proteomic-analysis
#18
Gloria Barbarani, Antonella Ronchi, Margherita Ruoppolo, Lucia Santorelli, Robert Steinfelder, Sudharshan Elangovan, Cristina Fugazza, Marianna Caterino
The Sox6 transcription factor is crucial for terminal maturation of definitive red blood cells. Sox6-null mouse fetuses present misshapen and nucleated erythrocytes, due to impaired actin assembly and cytoskeleton stability. These defects are accompanied with a reduced survival of Sox6(-/-) red blood cells, resulting in a compensated anemia. Sox6-overexpression in K562 cells and in human primary ex vivo erythroid cultures enhances erythroid differentiation and leads to hemoglobinization, the hallmark of erythroid maturation...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29074349/structure-optimization-of-tetrahydropyridoindole-based-aldose-reductase-inhibitors-improved-their-efficacy-and-selectivity
#19
Magdalena Majekova, Jana Ballekova, Marta Prnova, Milan Stefek
In our previous study, tetrahydropyridoindoles carboxymethylated in position 8 were identified as aldose reductase (ALR2) inhibitors with mild efficacy and selectivity yet with significant antioxidant activity. In the present study we proceeded with optimization of the tetrahydropyridoindole scaffold by shifting the carboxymethyl pharmacophore from position 8 to position 5, with the aim to improve the biological activity. Commercial databases were screened for the presence of tetrahydropyridoindoles carboxymethylated in position 5 and an experimental set of eight compounds was created...
December 15, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29049291/a-deep-convolutional-neural-network-for-classification-of-red-blood-cells-in-sickle-cell-anemia
#20
Mengjia Xu, Dimitrios P Papageorgiou, Sabia Z Abidi, Ming Dao, Hong Zhao, George Em Karniadakis
Sickle cell disease (SCD) is a hematological disorder leading to blood vessel occlusion accompanied by painful episodes and even death. Red blood cells (RBCs) of SCD patients have diverse shapes that reveal important biomechanical and bio-rheological characteristics, e.g. their density, fragility, adhesive properties, etc. Hence, having an objective and effective way of RBC shape quantification and classification will lead to better insights and eventual better prognosis of the disease. To this end, we have developed an automated, high-throughput, ex-vivo RBC shape classification framework that consists of three stages...
October 2017: PLoS Computational Biology
keyword
keyword
33479
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"