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https://www.readbyqxmd.com/read/27870069/suspected-myelodysplastic-myeloproliferative-neoplasm-in-a-feline-leukemia-virus-negative-cat
#1
Amy L Weeden, Kyle R Taylor, Scott P Terrell, Alexander E Gallagher, Heather L Wamsley
A 10-year-old castrated Domestic Short-Haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. The CBC revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. The cat tested negative for FeLV and feline immunodeficiency virus, but was positive for Mycoplasma haemominutum by PCR. Hematologic abnormalities were not responsive to therapy, so a repeat CBC and a bone marrow aspiration for cytology were performed...
November 21, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27825735/viral-diagnostic-criteria-for-feline-immunodeficiency-virus-and-feline-leukemia-virus-infections-in-domestic-cats-from-buenos-aires-argentina
#2
Sabrina Galdo Novo, Danilo Bucafusco, Leandro M Diaz, Ana Cristina Bratanich
A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR)...
November 5, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27748043/the-small-molecule-3g11-inhibits-hiv-1-reverse-transcription
#3
Silvana Opp, Thomas Fricke, Caitlin Shepard, Dmytro Kovalskyy, Akash Bhattacharya, Frank Herkules, Dmitri N Ivanov, Kim Baek, Jose Valle-Casuso, Felipe Diaz-Griffero
The small molecule 6-(tert-butyl)-4-phenyl-4-(trifluoromethyl)-1H,3H-1,3,5-triazin-2-one (3G11) inhibits HIV-1 replication in the human T cell line MT-2. Here we showed that 3G11 specifically and potently blocks HIV-1 infection. By contrast, 3G11 did not block other retroviruses such as HIV-2, simian immunodeficiency virus (SIVmac ), bovine immunodeficiency virus (BIV), feline immunodeficiency virus (FIV), equine infectious anemia virus (EIAV), N-tropic murine leukemia virus (N-MLV), B-tropic murine leukemia virus (B-MLV) and Moloney murine leukemia virus (Mo-MLV)...
October 17, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27549192/mutagenesis-of-n-terminal-residues-of-feline-foamy-virus-gag-reveals-entirely-distinct-functions-during-capsid-formation-particle-assembly-gag-processing-and-budding
#4
Yang Liu, Matthew J Betts, Janet Lei, Guochao Wei, Qiuying Bao, Timo Kehl, Robert B Russell, Martin Löchelt
BACKGROUND: Foamy viruses (FVs) of the Spumaretrovirinae subfamily are distinct retroviruses, with many features of their molecular biology and replication strategy clearly different from those of the Orthoretroviruses, such as human immunodeficiency, murine leukemia, and human T cell lymphotropic viruses. The FV Gag N-terminal region is responsible for capsid formation and particle budding via interaction with Env. However, the critical residues or motifs in this region and their functional interaction are currently ill-defined, especially in non-primate FVs...
2016: Retrovirology
https://www.readbyqxmd.com/read/27402672/maternal-iron-status-during-pregnancy-compared-with-neonatal-iron-status-better-predicts-placental-iron-transporter-expression-in-humans
#5
Cora M Best, Eva K Pressman, Chang Cao, Elizabeth Cooper, Ronnie Guillet, Olivia L Yost, Jonathan Galati, Tera R Kent, Kimberly O O'Brien
The placenta richly expresses nonheme and heme Fe transport proteins. To address the impact of maternal and neonatal Fe status and hepcidin on the regulation of these proteins, mRNA expression and protein abundance of nonheme and heme Fe transport proteins were evaluated in placental tissue from 154 adolescents. Regression analyses found maternal Fe status was significantly associated with multiple placental nonheme and heme transporters, whereas neonatal Fe status was related to only 3 heme transporters. Across statistical analyses, maternal Fe status was consistently associated with the placental nonheme Fe importer transferrin receptor 1 (TfR1)...
July 11, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27012922/prevalence-of-leishmania-infantum-and-co-infections-in-stray-cats-in-northern-italy
#6
Eva Spada, Ilaria Canzi, Luciana Baggiani, Roberta Perego, Fabrizio Vitale, Antonella Migliazzo, Daniela Proverbio
Stray cats in the city of Milan, Italy, were tested for Leishmania infantum and other selected infections. Twenty-seven cats (30.0%) were seroreactive by indirect fluorescent antibody test (IFAT), with an antibody titer of 1:40 for 16 (17.7%) cats and 1:80 (cut-off for feline L. infantum infection) for 11 (12.2%) cats. One blood (1.1%) and one popliteal lymph node (1.1%) sample tested positive by real-time polymerase chain reaction; no oculoconjunctival swabs tested positive. Feline immunodeficiency virus, feline leukemia virus, and feline coronavirus (FCoV) seroprevalence determined by enzyme-linked immunosorbent assay was 6...
April 2016: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/27006955/tlr-stimulation-dynamically-regulates-heme-and-iron-export-gene-expression-in-macrophages
#7
Mary Philip, Edison Y Chiu, Adeline M Hajjar, Janis L Abkowitz
Pathogenic bacteria have evolved multiple mechanisms to capture iron or iron-containing heme from host tissues or blood. In response, organisms have developed defense mechanisms to keep iron from pathogens. Very little of the body's iron store is available as free heme; rather nearly all body iron is complexed with heme or other proteins. The feline leukemia virus, subgroup C (FeLV-C) receptor, FLVCR, exports heme from cells. It was unknown whether FLVCR regulates heme-iron availability after infection, but given that other heme regulatory proteins are upregulated in macrophages in response to bacterial infection, we hypothesized that macrophages dynamically regulate FLVCR...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/26998822/-application-of-paramunity-inducers-in-small-animal-practice
#8
A L Proksch, K Hartmann
Paramunity inducers have been used to treat small animals for decades. Paramunity inducers are based on attenuated and inactivated poxviruses (avipox virus and parapox virus). Their applications include both therapeutic and prophylactic use in various diseases. Despite their wide and variable use, only a very small number of placebo-controlled studies has been published. Positive effects in preventing kitten mortality and in treating feline stomatitis have been reported, however, no statistically significant effect of their therapeutic use in canine parvovirus infection, feline leukemia infection virus infection or canine papillomavirus infection could be demonstrated...
2016: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/26965677/clinical-pathological-immunohistochemical-and-molecular-characterization-of-feline-chronic-gingivostomatitis
#9
(no author information available yet)
Veronica Machado Rolim, Saulo Petinatti Pavarini, Fabrício Souza Campos, Viviam Pignone, Cláudia Faraco, Marcelo de Souza Muccillo, Paulo Michel Roehe, Fernanda Viera Amorim da Costa, and David Driemeier J Feline Med Surg. Epub ahead of print 8 February 2016. DOI: 10.1177/1098612X16628578Readers should note that the OnlineFirst version of the article 'Clinical, pathological, immunohistochemical and molecular characterization of feline chronic gingivostomatitis' (published 8 February 2016) contains some errors in the abstract and the results section...
March 10, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/26933095/cutaneous-lymphoma-at-injection-sites-pathological-immunophenotypical-and-molecular-characterization-in-17-cats
#10
P Roccabianca, G Avallone, A Rodriguez, L Crippa, E Lepri, C Giudice, M Caniatti, P F Moore, V K Affolter
Feline primary cutaneous lymphomas (FPCLs) account for 0.2% to 3% of all lymphomas in cats and are more frequently dermal nonepitheliotropic small T-cell tumors. Emergence of FPCL seems unrelated to feline leukemia virus (FeLV) serological positivity or to skin inflammation. A total of 17 cutaneous lymphomas with a history of vaccine injection at the site of tumor development were selected from 47 FPCLs. Clinical presentation, histology, immunophenotype, FeLV p27 and gp70 expression, and clonality were assessed...
July 2016: Veterinary Pathology
https://www.readbyqxmd.com/read/26889025/novel-feline-leukemia-virus-interference-group-based-on-the-env-gene
#11
Ariko Miyake, Shinya Watanabe, Takahiro Hiratsuka, Jumpei Ito, Minh Ha Ngo, Isaac Makundi, Junna Kawasaki, Yasuyuki Endo, Hajime Tsujimoto, Kazuo Nishigaki
Feline leukemia virus (FeLV) subgroups have emerged in infected cats via the mutation or recombination of the env gene of subgroup A FeLV (FeLV-A), the primary virus. We report the isolation and characterization of a novel env gene, TG35-2, and report that the TG35-2 pseudotype can be categorized as a novel FeLV subgroup. The TG35-2 envelope protein displays strong sequence identity to FeLV-A Env, suggesting that selection pressure in cats causes novel FeLV subgroups to emerge.
May 2016: Journal of Virology
https://www.readbyqxmd.com/read/26858258/clinical-pathological-immunohistochemical-and-molecular-characterization-of-feline-chronic-gingivostomatitis
#12
Veronica Machado Rolim, Saulo Petinatti Pavarini, Fabrício Souza Campos, Viviam Pignone, Cláudia Faraco, Marcelo de Souza Muccillo, Paulo Michel Roehe, Fernanda Viera Amorim da Costa, David Driemeier
OBJECTIVES: This study presents the clinical, pathological, immunohistochemical and molecular characterization of 27 cats with feline chronic gingivostomatitis (FCG). METHODS: Oral mucosal biopsies, blood and swabs were collected from cats presenting with oral lesions. The tissue sections were submitted for histopathology and immunohistochemical analysis for feline calicivirus (FCV), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV). The swabs were subjected to PCR analysis for FCV, and blood for FeLV and FIV...
February 8, 2016: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/26747244/genotyping-of-feline-leukemia-virus-in-mexican-housecats
#13
Hugo Ramírez, Marcela Autran, M Martha García, M Ángel Carmona, Cecilia Rodríguez, H Alejandro Martínez
Feline leukemia virus (FeLV) is a retrovirus with variable rates of infection globally. DNA was obtained from cats' peripheral blood mononuclear cells, and proviral DNA of pol and env genes was detected using PCR. Seventy-six percent of cats scored positive for FeLV using env-PCR; and 54 %, by pol-PCR. Phylogenetic analysis of both regions identified sequences that correspond to a group that includes endogenous retroviruses. They form an independent branch and, therefore, a new group of endogenous viruses...
April 2016: Archives of Virology
https://www.readbyqxmd.com/read/26711049/molecular-characterization-and-virus-neutralization-patterns-of-severe-non-epizootic-forms-of-feline-calicivirus-infections-resembling-virulent-systemic-disease-in-cats-in-switzerland-and-in-liechtenstein
#14
Barbara Willi, Andrea M Spiri, Marina L Meli, Ayman Samman, Karolin Hoffmann, Titus Sydler, Valentino Cattori, Felix Graf, Kevin A Diserens, Isabelle Padrutt, Stefanie Nesina, Alice Berger, Maja Ruetten, Barbara Riond, Margaret J Hosie, Regina Hofmann-Lehmann
Feline calicivirus (FCV) infections are associated with oral ulceration, chronic stomatitis and a limping syndrome. Epizootic outbreaks of virulent systemic disease (VSD) have been reported in the USA and Europe. Here, the molecular characterization and neutralization patterns of FCV isolates from cases of severe, non-epizootic infection associated with skin ulceration and edema are presented. Samples from eleven symptomatic cats, four in-contact cats and 27 cats with no contact with symptomatic cats were collected and tested for FCV, feline herpesvirus-1 (FHV-1), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV)...
January 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/26689419/retroviral-dna-the-silent-winner-blood-transfusion-containing-latent-feline-leukemia-provirus-causes-infection-and-disease-in-na%C3%A3-ve-recipient-cats
#15
Stefanie Nesina, A Katrin Helfer-Hungerbuehler, Barbara Riond, Felicitas S Boretti, Barbara Willi, Marina L Meli, Paula Grest, Regina Hofmann-Lehmann
BACKGROUND: The feline leukemia virus (FeLV) is a gamma-retrovirus of domestic cats that was discovered half a century ago. Cats that are infected with FeLV may develop a progressive infection resulting in persistent viremia, immunodeficiency, tumors, anemia and death. A significant number of cats mount a protective immune response that suppresses viremia; these cats develop a regressive infection characterized by the absence of viral replication and the presence of low levels of proviral DNA...
2015: Retrovirology
https://www.readbyqxmd.com/read/26604265/reply-to-immunosuppression-in-a-comparative-study-of-feline-leukemia-virus-vaccines
#16
LETTER
M Patel, K Carritt, J Lane, H Jayappa, M Stahl, M Bourgeois
No abstract text is available yet for this article.
December 2015: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/26581999/ancestral-mutations-acquired-in-refrex-1-a-restriction-factor-against-feline-retroviruses-during-its-cooption-and-domestication
#17
Jumpei Ito, Takuya Baba, Junna Kawasaki, Kazuo Nishigaki
UNLABELLED: Endogenous retroviruses (ERVs) are remnants of ancestral retroviral infections of germ cells. Retroviral endogenization is an adaptation process for the host genome, and ERVs are gradually attenuated or inactivated by mutation. However, some ERVs that have been "domesticated" by their hosts eventually gain physiological functions, such as placentation or viral resistance. We previously reported the discovery of Refrex-1, a soluble antiretroviral factor in domestic cats that specifically inhibits infection by feline leukemia virus subgroup D (FeLV-D), a chimeric virus of FeLV, and a feline ERV, ERV-DC...
February 2016: Journal of Virology
https://www.readbyqxmd.com/read/26559384/causes-of-endogenous-uveitis-in-cats-presented-to-referral-clinics-in-north-carolina
#18
Maggie R Jinks, Robert V English, Brian C Gilger
OBJECTIVE: To investigate the causes of endogenous uveitis in cats presenting to referral ophthalmology clinics in North Carolina. PROCEDURE: Medical records of cats diagnosed with endogenous uveitis at North Carolina State University's College of Veterinary Medicine (NCSU-CVM) or Animal Eye Care Associates of Cary, NC between 2003 and 2015 were reviewed. Inclusion criteria were cats that had complete diagnostic workups, including clinical, clinicopathological, serological, and histopathological data, as well as imaging modalities...
July 2016: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/26387062/prevalence-of-selected-rickettsial-infections-in-cats-in-southern-germany
#19
Michèle Bergmann, Theresa Englert, Bianca Stuetzer, Jennifer R Hawley, Michael R Lappin, Katrin Hartmann
Prevalence of Anaplasma, Ehrlichia, Neorickettsia, and Wolbachia DNA in blood of 479 cats collected in different veterinary clinics in Southern Germany was determined using a previously published conventional PCR using 16S-23S intergenic spacer primers (5' CTG GGG ACT ACG GTC GCA AGA C 3' - forward; 5' CTC CAG TTT ATC ACT GGA AGT T 3' - reverse). Purified amplicons were sequenced to confirm genus and species. Associations between rickettsial infections, and feline immunodeficiency virus (FIV), as well as feline leukemia virus (FeLV) status were evaluated...
October 2015: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/26362646/toxoplasma-gondii-dirofilaria-immitis-feline-immunodeficiency-virus-fiv-and-feline-leukemia-virus-felv-infections-in-stray-and-pet-cats-felis-catus-in-northwest-china-co-infections-and-risk-factors
#20
Wei Cong, Qing-Feng Meng, Radu Blaga, Isabelle Villena, Xing-Quan Zhu, Ai-Dong Qian
This study was conducted to estimate the prevalence of Toxoplasma gondii, Dirofilaria immitis, feline immunodeficiency virus (FIV), and feline leukemia virus (FeLV) infections among stray and pet cats in Lanzhou, northwest China, and to identify the influence of age, gender, and regions on seropositivity. T. gondii antibodies were examined in cat sera by the modified agglutination test (MAT). The circulating antigens of D. immitis and FeLV and specific antibodies to FIV were examined using kits commercially available...
January 2016: Parasitology Research
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