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https://www.readbyqxmd.com/read/28923377/clinical-and-analytical-evaluation-of-the-new-aptima-mycoplasma-genitalium-assay-with-data-on-m-genitalium-prevalence-and-antimicrobial-resistance-in-m-genitalium-in-denmark-norway-and-sweden-in-2016
#1
M Unemo, K Salado-Rasmussen, M Hansen, A O Olsen, M Falk, D Golparian, M Aasterød, J Ringlander, C Steczkó Nilsson, M Sundqvist, K Schønning, H Moi, H Westh, J S Jensen
OBJECTIVES: Mycoplasma genitalium (MG) causes urethritis and cervicitis, potentially causing reproductive complications. Resistance in MG to first-line (azithromycin) and second-line (moxifloxacin) treatment has increased. We examined the clinical and analytical performance of the new CE/IVD Aptima Mycoplasma genitalium assay (CE/IVD AMG; Hologic); the prevalence of MG, Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG); and MG resistance to azithromycin and moxifloxacin in Denmark, Norway and Sweden in 2016...
September 15, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28899468/-pathogen-distribution-and-bacterial-resistance-in-children-with-severe-community-acquired-pneumonia
#2
Yun-Yun Lu, Rong Luo, Zhou Fu
OBJECTIVE: To investigate the distribution of pathogens and bacterial resistance in children with severe community-acquired pneumonia (CAP). METHODS: A total of 522 children with severe CAP who were hospitalized in 2016 were enrolled as study subjects. According to their age, they were divided into infant group (402 infants aged 28 days to 1 year), young children group (73 children aged 1 to 3 years), preschool children group (35 children aged 3 to 6 years), and school-aged children group (12 children aged ≥6 years)...
September 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28878004/mycoplasma-genitalium-macrolide-and-fluoroquinolone-resistance-detection-and-clinical-implications-in-a-selected-cohort-in-new-zealand
#3
Trevor Anderson, Edward Coughlan, Anja Werno
Mycoplasma genitalium has been associated with infections of the genitourinary tract and prevalence is secondary to Chlamydia trachomatis The clinical observation of increasing treatment failure indicating antibiotic resistance, especially in cases of recurrent urethritis, has been confirmed by molecular testing. Mutations in the 23S rRNA gene can cause macrolide resistance and topoisomerase/gyrase mutations can cause fluoroquinolone resistance. In this study, 115 M. genitalium DNA positive samples were analysed...
September 6, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28871927/sociodemographic-sexual-behavior-and-microbiological-profiles-of-men-attending-public-health-laboratories-for-testing-for-sexually-transmitted-diseases
#4
Mario Sviben, Sunčanica Ljubin-Sternak, Tomislav Meštrović, Jasmina Vraneš
In order to identify the groups at risk of sexually transmitted diseases (STDs), we assessed the sociodemographic profiles of men testing for STD, their sexual habits, and the results of microbiological analysis. During a three-year period, a total of 700 men older than 18 years of age completed the questionnaire regarding sociodemographic and sexual behavior. Urethral swabs were taken for microbiological analysis. Thirty-three percent of respondents reported not using condoms. Those that do not use condoms were predominantly less educated, unmarried but in steady relationships, employed, with children, and smokers...
July 2017: Acta Dermatovenerologica Croatica: ADC
https://www.readbyqxmd.com/read/28868962/prevalence-of-mycoplasma-genitalium-and-other-sexually-transmitted-infections-causing-urethritis-among-high-risk-heterosexual-male-patients-in-estonia
#5
Stanislav Tjagur, Reet Mändar, Margus Punab
BACKGROUND: We aimed to evaluate the prevalence of sexually transmitted infections (STI, including Mycoplasma genitalium, Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis) among high-risk heterosexual male patients and to clarify their potency to cause complaints and inflammation. METHODS: The study group included 825 men (18.0-49.5 y) consulting andrologist at Tartu University Hospital (Estonia) due to subjectively perceived risk of STI. Patients completed STI risk behaviour questionnaire...
September 4, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28867456/antimicrobial-activity-of-bitespiramycin-a-new-genetically-engineered-macrolide
#6
Weiqing He, Chunping Yang, Xiaofeng Zhao, Yiguang Wang
The antimicrobial activity of bitespiramycin (BT) against Chlamydia trachomatis (Ct), Chlamydia pneumoniae (Cp), Ureaplasma urealyticum (Uu), and Mycoplasma pneumoniae (Mp), was compared with those of azithromycin (AZM) and acetylspiramycin (AT-SP) in vitro. Furthermore, the anti-Mp activities of BT and AZM were evaluated in a hamster model. The activities of BT in vitro were similar to those of AZM but were more effective than those of AT-SP. BT effectively inhibited Mp infection at a dose of 200mg/kg in a hamster model...
August 24, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28838078/mycoplasma-genitalium-in-women-current-knowledge-and-research-priorities-for-this-recently-emerged-pathogen
#7
REVIEW
Harold C Wiesenfeld, Lisa E Manhart
Health consequences of sexually transmitted diseases disproportionately affect women, making it important to determine whether newly emerged pathogens cause sequelae. Although the pathogenic role of Mycoplasma genitalium in male urethritis is clear, fewer studies have been conducted among women to determine its pathogenic role in the female reproductive tract. Pelvic inflammatory disease (PID) is an important cause of infertility and ectopic pregnancy, and Chlamydia trachomatis and Neisseria gonorrhoeae are recognized microbial causes...
July 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28838077/the-unique-microbiology-and-molecular-pathogenesis-of-mycoplasma-genitalium
#8
REVIEW
Chris L McGowin, Patricia A Totten
Mycoplasma genitalium is increasingly appreciated as a common cause of sexually transmitted disease syndromes, including urethritis in men and cervicitis, endometritis, pelvic inflammatory disease, and possibly preterm birth, tubal factor infertility, and ectopic pregnancy in women. Despite these disease associations, which parallel those of Chlamydia trachomatis and Neisseria gonorrhoeae, the mechanisms by which this pathogen elicits inflammation, causes cellular damage, and persists in its only natural host (humans) are unique and are not fully understood...
July 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28838074/mycoplasma-genitalium-infection-in-men
#9
REVIEW
Patrick J Horner, David H Martin
Mycoplasmagenitalium is one of the major causes of nongonococcal urethritis (NGU) worldwide but an uncommon sexually transmitted infection (STI) in the general population. The risk of sexual transmission is probably lower than for Chlamydia trachomatis. Infection in men is usually asymptomatic and it is likely that most men resolve infection without developing disease. The incubation period for NGU caused by Mycoplasma genitalium is probably longer than for NGU caused by C. trachomatis. The clinical characteristics of symptomatic NGU have not been shown to identify the pathogen specific etiology...
July 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28813455/maternal-serum-c-reactive-protein-concentration-and-intra-amniotic-inflammation-in-women-with-preterm-prelabor-rupture-of-membranes
#10
Ivana Musilova, Marian Kacerovsky, Martin Stepan, Tomas Bestvina, Lenka Pliskova, Barbora Zednikova, Bo Jacobsson
OBJECTIVE: To evaluate maternal serum C-reactive protein (CRP) concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) in relation to the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: Two hundred and eighty-seven women with singleton pregnancies complicated by PPROM between 2014 and 2016 were included in this study. Maternal blood and amniotic fluid samples were collected at the time of admission...
2017: PloS One
https://www.readbyqxmd.com/read/28803864/the-detection-of-microorganisms-related-to-urethritis-from-the-oral-cavity-of-male-patients-with-urethritis
#11
Phuong Thi Le, Ryoichi Hamasuna, Masahiro Matsumoto, Keiichi Furubayashi, Masayuki Hatanaka, Shuichi Kawai, Takamasa Yamaguchi, Kazutaka Uehara, Norihiko Murakami, Masaru Yoshioka, Ken Nakayama, Yutaka Shiono, Keisuke Muraoka, Masahiko Suzuki, Naohiro Fujimoto, Tetsuro Matsumoto
OBJECTIVES: To investigate the presence of microorganisms related to urethritis in the oral cavity of male patients with urethritis and the efficacies of antimicrobials for urethritis on microorganisms in the oral cavity. METHODS: Ninety-two male patients with urethritis and 17 male controls participated to this study at 12 urology clinics in Japan between March 2014 and March 2015. The first voided urine (FVU) and oral wash fluid (OWF) specimens were collected from the participants...
October 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28757928/assessing-the-need-for-routine-screening-for-mycoplasma-genitalium-in-the-low-risk-female-population-a-prevalence-and-co-infection-study-on-women-from-croatia
#12
Sunčanica Ljubin-Sternak, Tomislav Meštrović, Branko Kolarić, Neda Jarža-Davila, Tatjana Marijan, Jasmina Vraneš
BACKGROUND: There is an ongoing debate regarding possible cost and benefits, but also harm of universal screening for the emerging sexually transmitted pathogen Mycoplasma genitalium. METHODS: From the initial pool of 8665 samples that were tested, a subset of Chlamydia trachomatis-positive and randomly selected C. trachomatis-negative cervical swabs were further interrogated for M. genitalium by real-time polymerase chain reaction, using a 224 bp long fragment of the glyceraldehyde-3-phosphate dehydrogenase gene...
2017: International Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28723470/sexually-transmitted-disease-and-male-infertility-a-systematic-review
#13
REVIEW
Mikkel Fode, Ferdinando Fusco, Larry Lipshultz, Wolfgang Weidner
CONTEXT: Theoretically, sexually transmitted diseases (STDs) have the potential to disrupt male fertility; however, the topic remains controversial. OBJECTIVE: To describe the possible association between STDs and male infertility and to explore possible pathophysiologic mechanisms. EVIDENCE ACQUISITION: We performed a systematic literature review in accordance with the PRISMA guidelines. PubMed, Embase, and the Cochrane Library were searched for articles published before January 1, 2016, using the MeSH terms for a variety of STDs and infertility...
October 2016: European Urology Focus
https://www.readbyqxmd.com/read/28722366/-large-vessel-vasculitis-pathogenesis-diagnostic-and-medical-management
#14
Alireza Sadrkhah, Jörg D Seebach, Yannick Muller
Large vessels vasculitis includes two diseases : 1) giant cell arteritis, formerly known as Horton's arteritis and 2) Takayasu arteritis. In this article, we will describe and compare the epidemiology, pathogenesis, diagnostic criteria and medical management of both vasculitis. T helper (Th) 1 and Th17 responses, genetic links and the role of viral (varicella zoster) and bacterial infection (Mycoplasma pneumoniae or Chlamydia pneumoniae) will be discussed. Classification criteria, inflammation biomarkers and progress in imaging tools will also be described...
April 5, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28717267/semen-culture-and-the-assessment-of-genitourinary-tract-infections
#15
REVIEW
Michael Solomon, Ralf Henkel
The male factor contributes approximately 50% to infertility-related cases in couples with an estimated 12%-35% of these cases attributable to male genital tract infections. Depending on the nature of the infection, testicular sperm production, sperm transport, and sperm function can be compromised. Yet, infections are potentially treatable causes of infertility. Male genital tract infections are increasingly difficult to detect. Moreover, they often remain asymptomatic ("silent") with the result that they are then passed on to the relevant sexual partner leading to fertilization and pregnancy failure as well as illness of the offspring...
July 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28698342/prevalence-and-antibiotic-resistance-of-mycoplasma-genitalium-among-sti-clinic-attendees-in-western-canada-a-cross-sectional-analysis
#16
Jennifer Gratrix, Sabrina Plitt, LeeAnn Turnbull, Petra Smyczek, Judith Brandley, Ron Scarrott, Prenilla Naidu, Penny Parker, Brenda Blore, Amy Bull, Sandy Shokoples, Lindsay Bertholet, Irene Martin, Max Chernesky, Ron Read, Ameeta Singh
OBJECTIVES: To determine the prevalence and correlates of Mycoplasma genitalium (MG) infection among men and women, determine the prevalence of gene mutations conferring resistance and compare test performance of female specimen types. METHODS: A cross-sectional study was conducted on specimens collected for gonorrhoea (NG, Neisseria gonorrhoeae) and chlamydia (CT, Chlamydia trachomatis) among male and female Alberta STI clinic attendees using the M. genitalium transcription-mediated amplification-research use only test...
July 10, 2017: BMJ Open
https://www.readbyqxmd.com/read/28697843/uriswab-an-effective-transport-medium-for-nucleic-acid-detection-of-chlamydia-trachomatis-mycoplasma-genitalium-and-neisseria-gonorrhoeae
#17
Anna-Maria G Costa, Suzanne M Garland, Rebecca Guy, Handan Wand, Sepehr N Tabrizi
Background: Patient self-sampling allows for remote collection and return to clinic or laboratory by post. Urine samples, although convenient, are challenging to post. This study evaluated UriSwab (Copan, Brescia, Italy) as a collection and transport vessel for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG) detection by polymerase chain reaction, compared with flocked swab and neat urine. Methods: Five replicates of each specimen type were prepared from previously characterised urine samples (n=330), stored at room temperature (RT) or 37°C, then extracted on day 1, 3, 7, 10 and 16 (VERSANT kPCR Sample Prep System, Siemens, Munich, Germany)...
July 12, 2017: Sexual Health
https://www.readbyqxmd.com/read/28681823/comparative-analysis-of-syndromic-case-management-and-polymerase-chain-reaction-based-diagnostic-assays-for-treatment-of-neisseria-gonorrhoeae-chlamydia-trachomatis-and-genital-mycoplasmas-in-patients-of-genitourinary-discharge
#18
Nazneen Arif, Sujeesh Sebastian, Neena Khanna, Seema Sood, Benu Dhawan
To respond to the situation of high prevalence and need for effective treatment for sexually transmitted infections (STIs) in low-resource settings, syndromic diagnostic approach was recommended by the World Health Organization and was adopted by National AIDS Control Organization at the primary health centre level. A retrospective study was undertaken in symptomatic patients attending an STI clinic to validate the syndromic approach for genitourinary discharge syndrome. For aetiological diagnosis, culture and/or polymerase chain reaction was used...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28670432/relationship-between-chlamydia-ureaplasma-mycoplasma-genital-detection-with-semen-concentration-and-motility-among-greek-men
#19
Ageliki Gerovassili, Ourania Marcandona, Byron Asimakopoulos, Vasilis Karavasilis, Maria Panopoulou, Alexandros Ikonomidis
One hundred and seventy two men at the State of Thessaly, Greece, inquiring semen analysis were enrolled in the study in order to investigate the incidence of Chlamydia, Ureaplasma and Mycoplasma (C-U-M) genera in respect to total sperm number (TSN), progressive motility (grades a and b) and total motility (grades a, b and c). Putative relation of C-U-M acquirement with sexual behavior was also investigated. Incidence of C-U-M among non-oligozoospermic and oligozoospermic men was similar. Νο correlation of C-U-M carriage to either oligozoospermia or asthenozoospermia was found...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28646898/prevalence-of-chlamydia-trachomatis-neisseria-gonorrhoeae-mycoplasma-genitalium-and-ureaplasma-urealyticum-infections-using-a-novel-isothermal-simultaneous-rna-amplification-testing-method-in-infertile-males
#20
Ling Qing, Qi-Xiang Song, Jian-Li Feng, Hai-Yan Li, Guiming Liu, Hai-Hong Jiang
BACKGROUND: The purpose of this study was to evaluate the prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium and Ureaplasma urealyticum infections in infertile men that consulted our outpatient departments using a novel simultaneous amplification testing (SAT) that is RNA-detection based. The possible impact of C. trachomatis, N. gonorrhoeae, M. genitalium and U. urealyticum infections on semen parameters was also noted in the present study. METHODS: A total of 2607 males that were diagnosed with infertility were included in this study...
June 24, 2017: Annals of Clinical Microbiology and Antimicrobials
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