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https://www.readbyqxmd.com/read/27898565/quantitative-real-time-polymerase-chain-reaction-for-the-diagnosis-of-mycoplasma-genitalium-infection-in-south-african-men-with-and-without-symptoms-of-urethritis
#1
Marie Cecilia le Roux, Anwar Ahmed Hoosen
BACKGROUND: This study was done to diagnose Mycoplasma genitalium infection based on bacterial load in urine specimens from symptomatic and asymptomatic men. METHODS: Urine specimens from 94 men with visible urethral discharge, 206 with burning on micturition and 75 without symptoms presenting to a family practitioner were tested for M. genitalium as well as Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis by transcription-mediated amplification assays...
November 28, 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27893600/prevalence-and-correlates-of-genital-infections-among-newly-diagnosed-human-immunodeficiency-virus-infected-adults-entering-human-immunodeficiency-virus-care-in-windhoek-namibia
#2
Gaston Djomand, Madeleine Schlefer, Steve Gutreuter, Sarah Tobias, Roopal Patel, Nickolas DeLuca, Julia Hood, Souleymane Sawadogo, Cheng Chen, Alexinah Muadinohamba, David W Lowrance, Naomi Bock
BACKGROUND: Identifying and treating genital infections, including sexually transmitted infections (STI), among newly diagnosed human immunodeficiency virus (HIV)-infected individuals may benefit both public and individual health. We assessed prevalence of genital infections and their correlates among newly diagnosed HIV-infected individuals enrolling in HIV care services in Namibia. METHODS: Newly diagnosed HIV-infected adults entering HIV care at 2 health facilities in Windhoek, Namibia, were recruited from December 2012 to March 2014...
November 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27859690/prevalence-and-distribution-of-chlamydia-trachomatis-genovars-in-indian-infertile-patients-a-pilot-study
#3
Jyoti Rawre, Benu Dhawan, Neena Malhotra, Vishnubhatla Sreenivas, Shobha Broor, Rama Chaudhry
To determine the prevalence and distribution of Chlamydia trachomatis genovars in patients with infertility by PCR-RFLP and ompA gene sequencing. Prevalence of other etiological agents (viz., Ureaplasma spp. and Mycoplasma hominis) were also assessed. Endocervical swabs were collected from 477 women and urine was collected from 151 men attending the Infertility Clinic. The samples were screened for C. trachomatis by cryptic plasmid, ompA gene and nested ompA gene PCR. Genotyping was performed by PCR-RFLP and sequencing...
December 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27836808/mycoplasma-genitalium-and-trichomonas-vaginalis-in-france-a-point-prevalence-study-in-people-screened-for-sexually-transmitted-diseases
#4
Sabine Pereyre, Cécile Laurier Nadalié, Cécile Bébéar
OBJECTIVES: Mycoplasma genitalium and Trichomonas vaginalis are common causes of sexually transmitted infections but limited prevalence data are available in France. We aimed to evaluate the prevalence of M. genitalium and T. vaginalis infections and to assess prevalence by gender, age, sample collection sites and clinical symptoms. A multicentre collection of specimens was intended to obtain a nationwide overview of the epidemiology. METHODS: Between September 2014 and January 2015, 2652 consecutive urogenital specimens submitted to the microbiology diagnostic departments of 16 French university hospitals for Chlamydia trachomatis and Neisseria gonorrhoeae detection were collected...
November 8, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27803507/role-of-atypical-bacteria-in-hospitalized-patients-with-nursing-home-acquired-pneumonia
#5
Laura Meyer-Junco
Background: Nursing home-acquired pneumonia (NHAP) has been identified as one of the leading causes of mortality and hospitalization for long-term care residents. However, current and previous pneumonia guidelines differ on the appropriate management of NHAP in hospitalized patients, specifically in regard to the role of atypical bacteria such as Chlamydiae pneumonia, Mycoplasma pneumoniae, and Legionella. Objectives: The purpose of this review is to evaluate clinical trials conducted in hospitalized patients with NHAP to determine the prevalence of atypical bacteria and thus the role for empiric antibiotic coverage of these pathogens in NHAP...
October 2016: Hospital Pharmacy
https://www.readbyqxmd.com/read/27753796/etiology-of-acute-respiratory-infections-in-infants-a-prospective-birth-cohort-study
#6
Prawin Kumar, Guruprasad R Medigeshi, Vishnu S Mishra, Mojahidul Islam, Shivani Randev, Aparna Mukherjee, Rama Chaudhry, Arti Kapil, Kana Ram Jat, Rakesh Lodha, Sushil K Kabra
BACKGROUND: There is paucity of studies on etiology of acute respiratory infections (ARI) in infants. OBJECTIVE: To document incidence and etiology of ARI in infants, their seasonal variability and association of clinical profile with etiology. MATERIAL AND METHODS: A birth cohort was followed for the first year of life; for each episode of ARI, nasopharyngeal aspirates were collected to identify the causative respiratory virus/es using multiplex real time PCR assay...
October 6, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27742734/pleuromutilins-potent-drugs-for-resistant-bugs-mode-of-action-and-resistance
#7
Susanne Paukner, Rosemarie Riedl
Pleuromutilins are antibiotics that selectively inhibit bacterial translation and are semisynthetic derivatives of the naturally occurring tricyclic diterpenoid pleuromutilin, which received its name from the pleuromutilin-producing fungus Pleurotus mutilus Tiamulin and valnemulin are two established derivatives in veterinary medicine for oral and intramuscular administration. As these early pleuromutilin drugs were developed at a time when companies focused on major antibacterial classes, such as the β-lactams, and resistance was not regarded as an issue, interest in antibiotic research including pleuromutilins was limited...
October 14, 2016: Cold Spring Harbor Perspectives in Medicine
https://www.readbyqxmd.com/read/27738813/-endometritis-rare-disease-with-clinical-importance
#8
S F Lax
Endometritis is nowadays rare in developed countries and typically shows a subclinical or mild course; therefore, there are probably more cases of endometritis than diagnosed but they lack clinical relevance. In the fertile period of life it can be the reason for vaginal bleeding and infertility. The most common causes for non-specific endometritis are residual placental tissue after abortion or childbirth, intrauterine interventions, lesions within the uterine cavity, such as endometrial polyps, endometrial hyperplasia and neoplasms, intrauterine devices (IUD) and cervical stenosis...
October 13, 2016: Der Pathologe
https://www.readbyqxmd.com/read/27728851/association-between-infectious-burden-socioeconomic-status-and-ischemic-stroke
#9
Frederick Palm, Pirkko J Pussinen, Annette Aigner, Heiko Becher, Florian Buggle, Matthias F Bauer, Caspar Grond-Ginsbach, Anton Safer, Christian Urbanek, Armin J Grau
BACKGROUND AND AIMS: Infectious diseases contribute to stroke risk, and are associated with socioeconomic status (SES). We tested the hypotheses that the aggregate burden of infections increases the risk of ischemic stroke (IS) and partly explains the association between low SES and ischemic stroke. METHODS: In a case-control study with 470 ischemic stroke patients and 809 age- and sex-matched controls, randomly selected from the population, antibodies against the periodontal microbial agents Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, against Chlamydia pneumonia, Mycoplasma pneumoniae (IgA and IgG), and CagA-positive Helicobacter pylori (IgG) were assessed...
October 5, 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27727592/to-study-the-association-between-anti-chlamydia-pneumoniae-antibodies-igg-anti-mycoplasma-pneumoniae-antibodies-igg-and-coronary-artery-disease-cad
#10
N S Kochar, S Garg, A Oberoi, S Chopra
No abstract text is available yet for this article.
January 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27717929/ultrasonographic-peculiarities-of-fetoplacental-complex-in-pregnancy-complicated-by-intrauterine-infection
#11
Nicholay A Shcherbina, Liudmyla A Vygovskaya
INTRODUCTION: the relevance of intrauterine infections is determined by significant peri- and postnatal loss as well as health impairment, which often results in disability and reduced quality of life. Ultrasonography is employed in order to provide a reliable assessment of the functional state of the fetoplacental system secondary to intrauterine fetal infection in the course of pregnancy. Ultrasound imaging is essential in diagnosis of various preclinical complications of pregnancy and detection of abnormalities in the developing fetus...
2016: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/27629896/expansion-of-comprehensive-screening-of-male-sexually-transmitted-infection-clinic-attendees-with-mycoplasma-genitalium-and-trichomonas-vaginalis-molecular-assessment-a-retrospective-analysis
#12
Erik Munson, David Wenten, Sheila Jhansale, Mary Kay Schuknecht, Nicki Pantuso, Joshua Gerritts, Aaron Steward, Kimber L Munson, Maureen Napierala, Deb Hamer
Of 1493 male sexually-transmitted infection (STI) clinic encounters in a high-prevalence STI community, 8.7% revealed detection of Chlamydia trachomatis and 6.6% revealed detection of Neisseria gonorrhoeae Additional Trichomonas vaginalis and Mycoplasma genitalium screening resulted in 17.4% and 23.9% of encounters positive for STI, respectively. STI agents were detected in 13.7% of urine specimens; addition of pharyngeal and rectal collections resulted in detection of STI agents in 19.0% and 23.9% of encounters, respectively...
September 14, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27617108/recruitment-of-minority-adolescents-and-young-adults-into-randomised-clinical-trials-testing-the-design-of-the-technology-enhanced-community-health-nursing-tech-n-pelvic-inflammatory-disease-trial
#13
Maria Trent, Shang-En Chung, Charlotte Gaydos, Kevin D Frick, Jennifer Anders, Steven Huettner, Richard Rothman, Arlene Butz
PURPOSE: Pelvic inflammatory disease (PID) disproportionately affects adolescent and young adult (AYA) women and can negatively influence reproductive health trajectories. Few randomized controlled trials (RCTs) have focused on strategies to improve outpatient adherence or to reduce reproductive morbidity in this population. This paper describes the research methods and preliminary effectiveness of recruitment, retention, and intervention strategies employed in a novel RCT designed to test a technology-enhanced community-health nursing (TECH-N) intervention among urban AYA with PID...
August 2016: European Medical Journal. Reproductive Health
https://www.readbyqxmd.com/read/27588273/study-of-the-prevalence-and-association-of-ocular-chlamydial-conjunctivitis-in-women-with-genital-infection-by-chlamydia-trachomatis-mycoplasma-genitalium-and-candida-albicans-attending-outpatient-clinic
#14
Rania Abdelmonem Khattab, Maha Mohssen Abdelfattah
AIM: To determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection. METHODS: This study was performed on 100 (50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27558163/urethral-inflammatory-response-to-ureaplasma-is-significantly-lower-than-to-mycoplasma-genitalium-and-chlamydia-trachomatis
#15
Harald Moi, Nils Reinton, Ivana Randjelovic, Elina J Reponen, Line Syvertsen, Amir Moghaddam
A non-syndromic approach to treatment of people with non-gonococcal urethritis (NGU) requires identification of pathogens and understanding of the role of those pathogens in causing disease. The most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of Mycoplasmataceae, in particular Ureaplasma urealyticum and Ureaplasma parvum To better understand the role of these Ureaplasma species in NGU, we have performed a prospective analysis of male patients voluntarily attending a drop in STI clinic in Oslo...
August 24, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/27542698/gardnerella-vaginalis-and-mollicute-detection-in-rectal-swabs-from-men-who-have-sex-with-men
#16
Ciara Cox, Alison P Watt, James P McKenna, Peter V Coyle
The numbers of rectal sexually transmitted infections are on the rise especially among men who have sex with men. Males from men who have sex with men population are encouraged to send a rectal swab to the laboratory for sexually transmitted infection screening at their visit to the Genitourinary Medicine Clinic. In healthy asymptomatic males, the range of pathogens tested is limited therefore other pathogens may be left untreated allowing infections to persist among sexual partners. Molecular techniques have revolutionarised sexually transmitted infection testing enabling the detection of previously difficult-to-culture pathogens in extra-genital sites and have increased the evidence base for their clinical significance...
August 19, 2016: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/27511901/the-prevalence-of-chlamydia-trachomatis-and-mycoplasma-genitalium-tubal-infections-and-their-effects-on-the-expression-of-il-6-and-leukaemia-inhibitory-factor-in-fallopian-tubes-with-and-without-an-ectopic-pregnancy
#17
Bassem Refaat, Ahmed Mohamed Ashshi, Sarah Abdullah Batwa, Jawwad Ahmad, Shakir Idris, Seham Yahia Kutbi, Faizah Ahmed Malibary, Fadi Fayez Kamfar
This was a prospective case-control study that measured the prevalence of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG) by an IVD CE multiplex PCR kit in fresh Fallopian tubes (FT) obtained from 96 ectopic pregnancies (EP) and 61 controls in the midluteal phase of the cycle. We later measured the expression profile of IL-6, leukaemia inhibitory factor (LIF) and their signalling molecules, in respect to the type and number of infections, by immunohistochemistry, ELISA and quantitative RT-PCR...
October 2016: Innate Immunity
https://www.readbyqxmd.com/read/27502694/mycoplasma-genitalium-macrolide-resistance-in-stockholm-sweden
#18
Eva Björnelius, Charlotta Magnusson, Jørgen Skov Jensen
OBJECTIVES: Mycoplasma genitalium is an important cause of STI in men and women. Worldwide evidence suggests a reduction in efficacy of azithromycin treatment due to the prevalence of macrolide resistant M. genitalium. The aim of this study was to describe the prevalence of macrolide resistance in patients with a positive test for M. genitalium within our setting. METHODS: Two STI clinics in Stockholm offered tests for M. genitalium as part of a routine care pathway...
August 8, 2016: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/27497595/performance-of-self-collected-penile-meatal-swabs-compared-to-clinician-collected-urethral-swabs-for-the-detection-of-chlamydia-trachomatis-neisseria-gonorrhoeae-trichomonas-vaginalis-and-mycoplasma-genitalium-by-nucleic-acid-amplification-assays
#19
Laura Dize, Perry Barnes, Mathilda Barnes, Yu-Hsiang Hsieh, Vincent Marsiglia, Della Duncan, Justin Hardick, Charlotte A Gaydos
Men were enrolled in a study to assess the performance and acceptability of self-collected penile meatal swabs as compared to clinician-collected urethral swabs for sexually transmitted infections (STIs). We expected penile-meatal swabs to perform favorably to urethral swabs for Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) detection by nucleic acid amplification assays (NAATs). Of 203 swab pairs tested; for CT, penile-meatal swab sensitivity was 96...
October 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27429850/association-of-human-papillomavirus-neisseria-gonorrhoeae-and-chlamydia-trachomatis-co-infections-on-the-risk-of-high-grade-squamous-intraepithelial-cervical-lesion
#20
André Lp de Abreu, Natália Malaguti, Raquel P Souza, Nelson S Uchimura, Érika C Ferreira, Monalisa W Pereira, Maria Db Carvalho, Sandra M Pelloso, Marcelo G Bonini, Fabrícia Gimenes, Marcia El Consolaro
The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods...
2016: American Journal of Cancer Research
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