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https://www.readbyqxmd.com/read/28419691/the-low-diverse-gastric-microbiome-of-the-jellyfish-cotylorhiza-tuberculata-is-dominated-by-four-novel-taxa
#1
Tomeu Viver, Luis H Orellana, Janet K Hatt, Mercedes Urdiain, Sara Díaz, Michael Richter, Josefa Antón, Massimo Avian, Rudolf Amann, Konstantinos T Konstantinidis, Ramon Rosselló-Móra
Cotylorhiza tuberculata is an important scyphozoan jellyfish producing population blooms in the Mediterranean probably due to pelagic ecosystem's decay. Its gastric cavity can serve as a simple model of microbial - animal digestive associations, yet poorly characterized. Using state-of-the-art metagenomic population binning and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), we show that only four novel clonal phylotypes were consistently associated with multiple jellyfish adults...
April 17, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28377334/mycoplasma-genitalium-a-mycoplasma-still-underestimated
#2
Julie Plantamura, Christine Bigaillon, Aurore Bousquet, Déborah Delaune, Sébastien Larréché, Sarah Bugier, Audrey Mérens, Cécile Ficko
Three men referred to the emergency department with suspected sexually transmitted infection like urethritis. After collection of several clinical specimens, they are sent home with a probabilistic treatment. Mycoplasma genitalium research is performed in first line, as Neisseria gonorrhoeae and Chlamydia trachomatis, and comes back positive. Patients are recalled in order to evaluate probabilistic treatment efficiency. M. genitalium, still underestimated because of its recent discovery, is responsible for 10 to 35% of non gonococcal acute and chronical urethritis...
April 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28365060/clinical-evaluation-of-a-new-single-tube-multiplex-reverse-transcription-pcr-assay-for-simultaneous-detection-of-11-respiratory-viruses-mycoplasma-pneumoniae-and-chlamydia-in-hospitalized-children-with-acute-respiratory-infections
#3
Meng-Chuan Zhao, Gui-Xia Li, Dan Zhang, Hang-Yu Zhou, Hao Wang, Shuo Yang, Le Wang, Zhi-Shan Feng, Xue-Jun Ma
Respiratory Pathogen 13 Detection Kit (13× kit) is able to simultaneously detect 11 respiratory viruses, Mycoplasma pneumoniae (MP) and Chlamydia in a single reaction. Using 572 Nasopharyngeal aspirates collected from hospitalized children, the clinical performance of 13× kit for detecting 11 respiratory viruses was evaluated in comparison with a routinely used 2-tube multiplex reverse transcription PCR assay (2-tube assay) at provincial Centers for Disease Control and Prevention in China. The clinical performance of 13× kit for detecting MP and Chlamydia was evaluated by commercial real-time quantitative PCR (qPCR) kits or sequencing...
March 22, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28334949/mycoplasma-hominis-ureaplasma-urealyticum-and-chlamydia-trachomatis-respiratory-colonization-in-malaysian-preterm-infants
#4
Khuen Foong Ng, Kah Kee Tan, Mi-Chelle Chok, Nurul Zamil Mohd Muzzamil, Pauline Choo, Umathevi Paramasivam, Marlindawati Mohd Ali, Jun Yan Goh, Wan Ying Gan
This prospective observational study aims to determine the incidence, predictors and clinical features of Mycoplasma hominis (MH), Ureaplasma urealyticum (UU) and Chlamydia trachomatis (CT) respiratory colonization in infants <37 weeks of gestation. A total of 200 preterm newborns admitted to a tertiary center in Malaysia between 2013 and 2015 for increased breathing effort had their respiratory secretions tested for these bacteria by polymerase chain reaction. Fifteen of the 200 (7.5%) infants were detected to have these organisms in their respiratory tracts...
March 2, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28301989/routine-testing-of-mycoplasma-genitalium-and-trichomonas-vaginalis
#5
Marieke L M Jenniskens, Jan H W Veerbeek, Koen L Deurloo, Erik J van Hannen, Steven F T Thijsen
BACKGROUND: Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) are common sexual transmitted infections (STI). However, most STI screening programmes do not include routinely detection of these pathogens. Consequently, epidemiological data about MG and TV in the general population is lacking. The current study aims to give insight into the prevalence of both infections, thereby guiding decisions whether testing for these pathogens should be included routinely. METHODS: Between February 2013 and August 2015, all samples sent to the laboratory of Diakonessenhuis Utrecht for STI testing (i...
June 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28282645/haemophilus-influenzae-isolated-from-men-with-acute-urethritis-its-pathogenic-roles-responses-to-antimicrobial-chemotherapies-and-antimicrobial-susceptibilities
#6
Shin Ito, Kyoko Hatazaki, Ken Shimuta, Hiromi Kondo, Kosuke Mizutani, Mitsuru Yasuda, Keita Nakane, Tomohiro Tsuchiya, Shigeaki Yokoi, Masahiro Nakano, Makoto Ohinishi, Takashi Deguchi
BACKGROUND: There have been few comprehensive studies on Haemophilus influenza-positive urethritis. METHODS: In this retrospective study, we enrolled 68 men with H. influenzae-positive urethritis, including coinfections with Neisseria gonorrhoeae, Chlamydia trachomatis, and/or genital mycoplasmas: 2, 3, 20, and 43 treated with ceftriaxone, levofloxacin, sitafloxacin, and extended-release azithromycin (azithromycin-SR), respectively. We assessed microbiological outcomes in 54 men and clinical outcomes in 46 with H...
April 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28260146/simultaneous-detection-and-identification-of-sti-pathogens-by-multiplex-real-time-pcr-in-genital-tract-specimens-in-a-selected-area-of-apulia-a-region-of-southern-italy
#7
Raffaele Del Prete, Luigi Ronga, Mirella Lestingi, Grazia Addati, Umberto Filippo Angelotti, Domenico Di Carlo, Giuseppe Miragliotta
PURPOSE: Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis (MH), Mycoplasma genitalium, Ureaplasma urealyticum (UU) and Ureaplasma parvum (UP) in specimens collected from female (SF) and male (SM) patients. METHODS: 1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex(TM) II STI-7, Seegene, Inc...
March 4, 2017: Infection
https://www.readbyqxmd.com/read/28257513/effect-of-mycoplasma-hominis-and-cytomegalovirus-infection-on-pregnancy-outcome-a-prospective-study-of-200-mongolian-women-and-their-newborns
#8
Byambaa Otgonjargala, Kathrin Becker, Gunchin Batbaatar, Sandag Tsogtsaikhan, Jamsranjav Enkhtsetseg, Altangerel Enkhjargal, Klaus Pfeffer, Ortwin Adams, Chimeddorj Battogtokh, Birgit Henrich
In Mongolia, diagnostic tests for the detection of the sexually transmitted mycoplasmas, ureaplasmas, Herpes simplex virus (HSV), and cytomegalovirus (CMV) are currently not routinely used in clinical settings and the frequency of these STIs are enigmatic. The prevalence of these STI pathogens were prospectively evaluated among 200 Mongolian pregnant women and their newborns and correlated with pregnancy outcome. TaqMan PCRs were used to detect bacterial and viral STI pathogens in pre-birth vaginal swabs of the pregnant women and in oral swabs of their newborns...
2017: PloS One
https://www.readbyqxmd.com/read/28255605/an-epidemiological-study-on-vaginitis-in-6-150-women-of-reproductive-age-in-shanghai
#9
Haixia Wang, Zhonghua Huang, Zhaoxia Wu, Xulin Qi, Dongfang Lin
The aim of the present study was to determine the prevalence and risk factors for vaginitis, the proportion of pathogens, and cognition of reproductive age patients on the harmful effects of vaginitis, its risk factors, and treatment. This retrospective study enrolled 6,150 patients admitted to the Shanghai Jinshan Central Hospital from 2011 to 2015 with a chief complaint of abnormal vaginal discharge. A questionnaire was designed to survey the cognition of patients on the harmful effects of vaginitis. Routine gynecological examinations and laboratory tests were performed and the risk factors for contracting vaginitis were analyzed by multi-factor logistic regression analysis...
March 3, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28251179/fluorocycline-tp-271-is-potent-against-complicated-community-acquired-bacterial-pneumonia-pathogens
#10
Trudy H Grossman, Corey Fyfe, William O'Brien, Meredith Hackel, Mary Beth Minyard, Ken B Waites, Jacques Dubois, Timothy M Murphy, Andrew M Slee, William J Weiss, Joyce A Sutcliffe
TP-271 is a novel, fully synthetic fluorocycline antibiotic in clinical development for the treatment of respiratory infections caused by susceptible and multidrug-resistant pathogens. TP-271 was active in MIC assays against key community respiratory Gram-positive and Gram-negative pathogens, including Streptococcus pneumoniae (MIC90 = 0.03 µg/ml), methicillin-sensitive Staphylococcus aureus (MSSA; MIC90 = 0.25 µg/ml), methicillin-resistant S. aureus (MRSA; MIC90 = 0.12 µg/ml), Streptococcus pyogenes (MIC90 = 0...
January 2017: MSphere
https://www.readbyqxmd.com/read/28238212/development-a-rapid-and-accurate-multiplex-real-time-pcr-method-for-the-detection-chlamydia-trachomatis-and-mycoplasma-hominis
#11
Roya Safarkar, Jalil Fallah Mehrabadi, Zahra Noormohammadi, Reza Mirnejad
BACKGROUND: Sexually transmitted diseases easily spread among sexually active people and often have no symptoms. Rapid and accurate method for detecting these infections are necessary in early stages. The traditional detection methods of them are difficult and time-consuming. METHODS: In this study, multiplex real time PCR was optimized for rapid identification of Chlamydia trachomatis and Mycoplasma hominis in a single tube and was performed with our designed primers...
February 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28178118/microscopy-of-stained-urethral-smear-in-male-urethritis-which-cutoff-should-be-used
#12
Harald Moi, Usha Hartgill, Kristin Helene Skullerud, Elina J Reponen, Line Syvertsen, Amir Moghaddam
BACKGROUND: The microscopical diagnosis of male urethritis was recently questioned by Rietmeijer and Mettenbrink, lowering the diagnostic criteria of the diagnosis to ≥2 polymorphonuclear leucocytes (PMNL) per high power field (HPF), and adopted by Centers for Disease Control and Prevention in their 2015 STD Treatment Guidelines. The European Non-Gonococcal Urethritis Guideline advocates a limit of ≥5 PMNL/HPF. OBJECTIVE: To determine if syndromic treatment of urethritis should be considered with a cutoff value of ≥2 PMNL/HPF in urethral smear...
March 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28159916/impact-of-deploying-multiple-point-of-care-tests-with-a-sample-first-approach-on-a-sexual-health-clinical-care-pathway-a-service-evaluation
#13
Emma M Harding-Esch, Achyuta V Nori, Aseel Hegazi, Marcus J Pond, Olanike Okolo, Anthony Nardone, Catherine M Lowndes, Phillip Hay, S Tariq Sadiq
OBJECTIVES: To assess clinical service value of STI point-of-care test (POCT) use in a 'sample first' clinical pathway (patients providing samples on arrival at clinic, before clinician consultation). Specific outcomes were: patient acceptability; whether a rapid nucleic acid amplification test (NAAT) for Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) could be used as a POCT in practice; feasibility of non-NAAT POCT implementation for Trichomonas vaginalis (TV) and bacterial vaginosis (BV); impact on patient diagnosis and treatment...
February 3, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28152632/cervical-fluid-calreticulin-and-cathepsin-g-in-pregnancies-complicated-by-preterm-prelabor-rupture-of-membranes
#14
Ctirad Andrys, Ivana Musilova, Marcela Drahosova, Ondrej Soucek, Lenka Pliskova, Bo Jacobsson, Nanbert Zhong, Marian Kacerovsky
OBJECTIVE: The study aimed to determine the cervical calreticulin and cathepsin-G concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI). METHODS: Eighty women with singleton pregnancies complicated by PPROM were included in this study. Cervical and amniotic fluids were obtained at the time of admission, and concentrations of calreticulin and cathepsin-G in cervical fluid were determined using ELISA...
February 28, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28129377/high-prevalence-of-mycoplasma-pneumoniae-and-chlamydia-pneumoniae-in-children-with-acute-respiratory-infections-from-lima-peru
#15
Juana Del Valle-Mendoza, Fiorella Orellana-Peralta, Alvaro Marcelo-Rodríguez, Eduardo Verne, Mónica Esquivel-Vizcarra, Wilmer Silva-Caso, Miguel Angel Aguilar-Luis, Pablo Weilg, Verónica Casabona-Oré, Claudia Ugarte, Luis J Del Valle
BACKGROUND: Mycoplasma pneumoniae and Chlamydia pneumoniae are atypical pathogens responsible for pneumonia and a leading cause of morbidity and mortality in low income countries. The study objective is to determine the prevalence of this pathogens in Peruvian children with acute respiratory infections. METHODS: A consecutive cross-sectional study was conducted in Lima, Peru from May 2009 to September 2010. A total of 675 children admitted with clinical diagnoses of acute respiratory infections were tested for Mycoplasma pneumoniae and Chlamydia pneumoniae detection by polymerase chain reaction (PCR), and clinical symptoms were registered by the attending physician...
2017: PloS One
https://www.readbyqxmd.com/read/28120647/detection-of-sexually-transmitted-pathogens-in-patients-with-chronic-prostatitis-chronic-pelvic-pain-a-prospective-clinical-study
#16
Dino Papeš, Miram Pasini, Ana Jerončić, Martina Vargović, Viktor Kotarski, Alemka Markotić, Višnja Škerk
In <10% of patients with prostatitis syndrome, a causative uropathogenic organism can be detected. It has been shown that certain organisms that cause sexually transmitted infections can also cause chronic bacterial prostatitis, which can be hard to diagnose and treat appropriately because prostatic samples obtained by prostatic massage are not routinely tested to detect them. We conducted a clinical study to determine the prevalence of Chlamydia, mycoplasma, and trichomonas infection in 254 patients that were previously diagnosed and treated for chronic prostatitis/chronic pelvic pain syndrome due to negative urethral swab, urine, and prostate samples...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28112332/-molecular-detection-of-sexually-transmitted-agents-in-a-symptomatic-group-of-men-and-its-relationship-with-sexual-behavior
#17
Daniela León, Javier Retamal, Ramón Silva, Carmen Ili, Stephanie Mieville, Pablo Guzmán, Gastón Briceño, Priscilla Brebi
BACKGROUND: Sexually transmitted infections (STIs) affect sexual and reproductive health of millions of men. Pathogens such as human papillomavirus (HPV), herpes simplex virus type 1 and 2 (HSV-1 y HSV-2), Chlamydia trachomatis,Mycoplasmagenitalium,Mycoplasma hominis and Ureaplasma urealyticum are associated with STIs. AIM: To detect pathogens associated with STIs in symptomatic men and its relationship with sexual behavior. METHODOLOGY: DNA was obtained from exfoliated cells of penis from 20 symptomatic men...
October 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28099135/mycoplasma-ureaplasma-infection-in-pregnancy-to-screen-or-not-to-screen
#18
Gilbert G G Donders, Kateryna Ruban, Gert Bellen, Ljubomir Petricevic
Mycoplasmata have been linked to pregnancy complications and neonatal risk. While formerly a limited number of species could be discovered by cultures, molecular biology nowadays discovers both lower quantities and more diverse species, making us realize that mycoplasmata are ubiquitous in the vaginal milieu and do not always pose a danger for pregnant women. As the meaning of mycoplasmata in pregnancy is not clear to many clinicians, we summarized the current knowledge about the meaning of different kinds of mycoplasmata in pregnancy and discuss the potential benefits and disadvantages of treatment...
January 18, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28079749/should-female-partners-of-men-with-non-gonococcal-urethritis-negative-for-chlamydia-trachomatis-and-mycoplasma-genitalium-be-informed-and-treated-clinical-outcomes-from-a-partner-study-of-heterosexual-men-with-ngu
#19
Jason J Ong, Angela Sarumpaet, Eric P F Chow, Catriona Bradshaw, Marcus Chen, Tim Read, Christopher K Fairley
BACKGROUND: To determine if female partners of men with pathogen-negative non-gonococcal urethritis (NGU) are at risk of genital infection. METHODS: Secondary data analysis using health records from a large sexually transmitted disease clinic in Melbourne of 1710 men and their female partners attending on the same day from January 2006 to April 2015. Proportions of female partners with symptoms suggesting genital infection or pelvic inflammatory disease (PID) were determined for: (1) men with NGU and no Chlamydia trachomatis or Mycoplasma genitalium (referred to as pathogen-negative NGU) (n = 91); 2) men with urethral C...
February 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28032361/relationship-between-chlamydia-trachomatis-and-mycoplasma-genitalium-infection-and-pregnancy-rate-and-outcome-in-iranian-infertile-couples
#20
L Dehghan Marvast, A Aflatoonian, A R Talebi, A Eley, A A Pacey
The study was performed to investigate the prevalence of Chlamydia trachomatis and Mycoplasma genitalium in a population of infertile couples from Iran and how this relates to tubal factor infertility, pregnancy rate and outcome of pregnancy. Blood, semen and first-void urine samples were obtained from 250 infertile couples and 250 fertile women as a control. Infertile couples were followed up after 24 months to determine diagnosis, referral for assisted conception, any pregnancy and pregnancy outcome. Data were analysed with regard to the results of (i) serological analysis for specific antibodies to C...
December 29, 2016: Andrologia
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