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https://www.readbyqxmd.com/read/29782292/evaluation-of-symptomatic-patients-with-resistant-discharge
#1
Cemile Sonmez, Selma Usluca, Ismail Hakki Usluca, Irem Kalipci, Figen Sezen, Cemal Resat Atalay, Selcuk Kilic
The aim of this study was to detect the presence of Chlamydia trachomatis, Neisseria (N.) gonorrhoeae, Mycoplasma (M.) hominis, M. genitalium, Ureaplasma (U.) urealyticum, and Trichomonas (T.) vaginalis in patients with resistant discharge. The study also evaluated the concordance of the diagnostic tests. Samples from 156 patients were tested by direct microscopy and culture for T. vaginalis and Mycoplasma IES for M. hominis and U. urealyticum. Multiplex Polymerase Chain Reaction (PCR) was used to determine the presence of six agents...
April 2018: Acta Dermatovenerologica Croatica: ADC
https://www.readbyqxmd.com/read/29729635/an-integrative-investigation-of-the-therapeutic-mechanism-of-ainsliaea-fragrans-champ-in-cervicitis-using-liquid-chromatography-tandem-mass-spectrometry-based-on-a-rat-plasma-metabolomics-strategy
#2
Xiaoyong Zhang, Mingzhen He, Shuangxia Lei, Bei Wu, Ting Tan, Hui Ouyang, Wen Xu, Yulin Feng
Cervicitis is an extremely common gynecological disease and can be induced by diverse factors such as Neisseria gonorrhoeae, Chlamydia trachomatis, and Mycoplasma genitalium infections. Long-term unhealed cervicitis may lead to a series of diseases including endometritis, salpingitis, pelvic inflammatory disease, and chorioamnionitis. However, the pathogenesis of cervicitis remains unknown. Ainsliaea fragrans Champ. (AFC) has been widely used in clinical treatment of cervicitis. In the present study, we performed an integrative investigation involving histopathology analysis and non-target plasma metabolomics analysis in a cervicitis rat model induced by phenol mucilage, using ultra-performance liquid chromatography coupled with a tandem quadrupole time-of-flight mass spectrometry approach...
April 27, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29729331/the-vaginal-microbiota-and-its-association-with-human-papillomavirus-chlamydia-trachomatis-neisseria-gonorrhea-and-mycoplasma-genitalium-infections-a-systematic-review-and-meta-analysis
#3
REVIEW
Jeanne Tamarelle, Anne C M Thiébaut, Bertille de Barbeyrac, Cécile Bébéar, Jacques Ravel, Elisabeth Delarocque-Astagneau
OBJECTIVES: The vaginal microbiota may modulate susceptibility to Human papillomavirus, Chlamydia trachomatis, Neisseria gonorrhea and Mycoplasma genitalium infections. The objective of this meta-analysis was to evaluate the association between these infections and the vaginal microbiota. METHODS: The search (2000-2016) yielded 1054 articles, of which 39 articles meeting the inclusion criteria were analyzed. The vaginal microbiota was dichotomized into high-Lactobacillus vaginal microbiota (HL-VMB) and low-Lactobacillus vaginal microbiota (LL-VMB), using either Nugent score, Amsel's criteria, presence of clue cells or gene sequencing...
May 2, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29706149/management-of-sexually-transmissible-infections-in-the-era-of-multiplexed-molecular-diagnostics-a-primary-care-survey
#4
Tal Brosh-Nissimov, Ron Kedem, Nimrod Ophir, Omri Shental, Nathan Keller, Sharon Amit
Background: Data regarding sexually transmissible infections (STI) often originate from STI clinics, screening programs or laboratory-based studies, thus are biased for specific risk groups or lack clinical details. This real-life observational study presents sample data of most young adult Israeli population by exploiting the centralised diagnostic and documentation platforms resulting from a mandatory military service at the age of 18 years for both genders. Methods: All STI diagnoses of Israeli Defence Forces soldiers during a 6-month period were reviewed...
April 30, 2018: Sexual Health
https://www.readbyqxmd.com/read/29698421/prevalence-of-mycoplasma-genitalium-in-men-with-urethritis-in-a-large-public-hospital-in-brussels-belgium-an-observational-cross-sectional-study
#5
Agnès Libois, Marie Hallin, Tania Crucitti, Marc Delforge, Stéphane De Wit
BACKGROUND: Mycoplasma genitalium (MG) is a cause of urethritis. While resistance to azithromycin is increasing, routine detection of MG is not performed in Belgium, where its prevalence is unknown. The aim of this study is to determine prevalence of MG in men with urethritis. METHOD AND FINDINGS: An "in-house" amplification assay detecting MG was performed on urine of men with complaints of urethritis who consulted the emergency unit or the Sexually Transmitted Infection clinic of our public hospital in Brussels...
2018: PloS One
https://www.readbyqxmd.com/read/29690685/-current-status-of-diagnosis-and-treatment-of-community-acquired-pneumonia-in-shanghai-revealed-by-a-questionnaire-analysis
#6
J Liu, J Zhang, Q J Cheng, J F Xu, Z J Jie, Y Jiao, Y Huang, J M Qu
Objective: To understand the current status of diagnosis and treatment of community-acquired pneumonia (CAP) among doctors in various hospitals across Shanghai, for the purpose of promoting the 2016 clinical practice guidelines for adult CAP of China. Methods: A questionnaire was designed to address the common questions in CAP management. The responses were collected via WeChat and the data were analyzed. Results: A total of 1 254 valid questionnaires were received, 46.1% from tertiary , 26.4% from secondary and 27...
April 12, 2018: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/29666749/prevalence-of-hiv-and-sexually-transmitted-infections-among-young-women-engaged-in-sex-work-aboard-foreign-fishing-vessels-in-kiribati
#7
Tebuka Toatu, Paul White, Damian Hoy, Dennie Iniakwala, Onofre Edwin A Merrilles, Sameer Vali Gopalani
Objective: To assess the prevalence of HIV and sexually transmitted infections (STIs) among women who board foreign fishing vessels for sex work in Kiribati. Methods: A cross-sectional study was designed to collect data on behavioural risk factors for STIs and knowledge of and attitudes towards HIV and STIs during 2007. Urine and blood samples were collected to test for HIV and select STIs. Descriptive statistics were performed for sociodemographic and behavioural characteristics, and χ2 tests were used to assess associations between potential key determinants and the presence of genital Chlamydia infection...
January 2018: Western Pacific Surveillance and Response Journal: WPSAR
https://www.readbyqxmd.com/read/29629645/development-and-application-of-two-multiplex-real-time-pcr-assays-for-detection-and-speciation-of-bacterial-pathogens-in-the-koala
#8
Lyndal S Hulse, Danica Hickey, Jessica M Mitchell, Kenneth W Beagley, William Ellis, Stephen D Johnston
Infectious diseases have contributed to the decline in the health of koala ( Phascolarctos cinereus) populations in the wild in some regions of Australia. Herein we report the development and validation of 2 multiplex real-time PCR (rtPCR) panels for the simultaneous detection of Mycoplasma spp., Ureaplasma spp., Bordetella bronchiseptica, and Chlamydia, including speciation and quantification of Chlamydia, in ocular, reproductive, and nasal swab samples in addition to semen and male urogenital and reproductive tissues, from koalas...
April 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29620787/distribution-of-genital-mollicutes-in-the-vaginal-ecosystem-of-women-with-different-clinical-conditions
#9
Claudio Foschi, Melissa Salvo, Antonietta D'Antuono, Valeria Gaspari, Nicoletta Banzola, Roberto Cevenini, Antonella Marangoni
Ureaplasma urelayticum (UU), Ureaplasma parvum (UP), Mycoplasma hominis (MH) and Mycoplasma genitalium (MG) are the most common Mollicutes of the female genital tract. Although many studies have addressed their possible role in the vaginal ecosystem, many aspects remain to be elucidated. The aim of this study was to evaluate the vaginal presence of ureaplasmas/mycoplasmas in women with different clinical conditions. By means of quantitative PCR assays, the prevalence and load of each Mollicute were assessed in different groups of pre-menopausal women: 'healthy' (n=29), women with bacterial vaginosis (BV) (n=21), patients with Chlamydia trachomatis (CT) infection (n=25) and subjects with vulvo-vaginal candidiasis (VVC) (n=23)...
April 5, 2018: New Microbiologica
https://www.readbyqxmd.com/read/29619908/prevalence-of-sexually-transmitted-diseases-in-asymptomatic-renal-transplant-recipients
#10
Mehmet Sarier, Nevgun Sepin Ozen, Hicran Guler, Ibrahim Duman, Yücel Yüksel, Sabri Tekin, Asuman Havva Yavuz, Levent Yucetin, Mine Erdogan Yilmaz
OBJECTIVES: Sexually transmitted diseases, which may be asymptomatic, have the potential to cause serious health problems in renal transplant recipients. The aim of this study was to determine the prevalence of sexually transmitted diseases in sexually active asymptomatic renal transplant patients by using real-time multiplex polymerase chain reaction assays. MATERIALS AND METHODS: This prospective controlled study was conducted between November 2016 and January 2017 in our hospital...
April 4, 2018: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29605219/bacterial-pneumonia-in-kidney-transplant-recipients
#11
REVIEW
D Wilmes, E Coche, H Rodriguez-Villalobos, N Kanaan
Bacterial pathogens are the most frequent cause of pneumonia after transplantation. Early after transplantation, recipients are at higher risk for nosocomial infections. The most commonly encountered pathogens during this period are gram-negative bacilli (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa …), but gram-positive coccus such as Staphylococcus aureus or Streptococcus pneumoniae and anaerobic bacteria can also be found. Empirical antibiotic therapy should be guided by previous colonisation of the recipient and bacterial resistance pattern in the hospital...
April 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29599387/clinical-and-sexual-risk-correlates-of-mycoplasma-genitalium-in-urban-pregnant-and-non-pregnant-young-women-cross-sectional-outcomes-using-the-baseline-data-from-the-women-s-biohealth-study
#12
Maria Trent, Jenell S Coleman, Justin Hardick, Jamie Perin, Lisa Tabacco, Steven Huettner, Jocelyn Ronda, Rebecca Felter-Wernsdorfer, Charlotte A Gaydos
OBJECTIVE: Research exploring the clinical and sexual risk correlates is essential to define universal standards for screening and management for Mycoplasma genitalium (MG). The objective of this study is to determine the baseline prevalence of MG and associated clinical risks using cross-sectional data. METHODS: Adolescent and young adult women 13-29 years were recruited during clinical visits during which biological specimens were collected for Neisseriagonorrhoeae (NG) and Chlamydia trachomatis (CT) testing to provide vaginal specimens for MG and Trichomonasvaginalis (TV) testing...
March 29, 2018: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29593057/multicenter-evaluation-of-the-biofire-filmarray-respiratory-panel-2-for-the-detection-of-viruses-and-bacteria-in-nasopharyngeal-swab-samples
#13
Amy L Leber, Kathy Everhart, Judy A Daly, Aubrey Hopper, Amanda Harrington, Paul Schreckenberger, Kathleen McKinley, Matthew Jones, Kristen Holmberg, Bart Kensinger
The FilmArray® Respiratory Panel 2 (RP2) is a multiplex in vitro diagnostic test for the simultaneous and rapid (∼45 minutes) detection of 22 pathogens directly from nasopharyngeal swab (NPS) samples. It contains updated (and in some instances redesigned) assays that improve upon the FilmArray® Respiratory Panel (RP; version 1.7), with a faster run time. The organisms identified are adenovirus, coronavirus 229E, coronavirus HKU1, coronavirus NL63, coronavirus OC43, human metapneumovirus, human rhinovirus/enterovirus, influenza A, influenza A H1, influenza A H1-2009, influenza A H3, influenza B, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, parainfluenza virus 4, respiratory syncytial virus, Bordetella pertussis , Chlamydia pneumoniae , and Mycoplasma pneumoniae...
March 28, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29573404/correlation-of-natural-autoantibodies-and-cardiovascular-disease-related-antibacterial-antibodies-in-pericardial-fluid-of-cardiac-surgery-patients
#14
D Simon, O Gilicze, N Farkas, J Najbauer, P Németh, L Lénárd, T Berki
Our previous studies showed that anti-citrate synthase (a-CS) IgM natural autoantibodies are present in healthy individuals without previous antigen stimulation, but no studies have investigated their presence in the pericardial fluid (PF). Therefore we detected the natural anti-CS IgG/M autoantibody levels in plasma and PF of cardiac surgery patients and investigated their relationship with cardiovascular disease-associated bacterial pathogens. PF and blood samples of 22 coronary artery bypass graft (CABG) and 10 aortic valve replacement (AVR) patients were tested for total Ig levels, natural autoantibodies and infection-related antibodies using ELISA and Luminex methods...
March 24, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29567802/-mycoplasma-genitalium-high-prevalence-of-resistance-to-macrolides-and-frequent-anorectal-infection-in-men-who-have-sex-with-men-in-western-sydney
#15
Deborah L Couldwell, Dean Jalocon, Melissa Power, Neisha J Jeoffreys, Sharon C-A Chen, David A Lewis
OBJECTIVES: We aimed to estimate the prevalence of Mycoplasma genitalium infection and of mutations linked to macrolide resistance using the ResistancePlus MG assay (SpeeDx, Sydney, New South Wales, Australia) in first-void urine (FVU), anorectal and oropharyngeal samples from men who have sex with men (MSM) attending Western Sydney Sexual Health Centre (WSSHC). METHODS: Consecutive symptomatic and asymptomatic MSM attending for STI testing were prospectively enrolled...
March 22, 2018: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29563165/risk-factors-for-mycoplasma-genitalium-endometritis-and-incident-infection-a-secondary-data-analysis-of-the-t-cell-response-against-chlamydia-trac-study
#16
Brandie D Taylor, Xiaojing Zheng, Catherine M O'Connell, Harold C Wiesenfeld, Sharon L Hillier, Toni Darville
OBJECTIVE: Assess risk factors for incident and endometrial Mycoplasma genitalium infection and determine if M. genitalium is associated with histological endometritis, an indicator of pelvic inflammatory disease. METHODS: This study was a secondary data analysis within the T cell Response Against Chlamydia (TRAC) Study, a prospective evaluation of 246 women with or at risk for Chlamydia trachomatis from urban outpatient clinics, who were followed quarterly for 12 months...
March 21, 2018: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29559392/identifying-a-consensus-sample-type-to-test-for-chlamydia-trachomatis-neisseria-gonorrhoeae-mycoplasma-genitalium-trichomonas-vaginalis-and-human-papillomavirus
#17
Liselotte Coorevits, Ans Traen, Luk Bingé, Jo Van Dorpe, Marleen Praet, Jerina Boelens, Elizaveta Padalko
OBJECTIVES: Sexually transmitted infections (STIs) are a global cause of acute illness. Early detection plays a crucial role in interrupting transmission and preventing complications. However, the accessibility of STI testing is curbed by the lack of an overall preferred sample type. By means of a prospective study in female sex workers (FSW), we compared the sensitivity of samples from different anatomical sites in detecting Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma genitalium and human papillomavirus...
March 17, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29553074/-validation-of-the-kit-for-detecting-mycoplasma-genitalium-from-the-male-urethritis
#18
Ryoichi Hamasuna, Masahiro Matsumoto, Phuong Thi LE, Naohiro Fujimoto, Tetsuro Matsumoto
Mycoplasma genitalium is one of the pathogenic microorganisms in male urethritis as a sexually transmitted infection (STI). M.genitalium is detected in the urine specimens of 15-25% male patients with urethritis. The emergence of macrolide- or fluoroquinolone-resistant M.genitalium has become a serious problem in the treatment of male urethritis worldwide, but there is no commercial-based detecting kits accepted by the national insurance in Japan. In this study, we tested the validity of a molecular kit for detecting seven microorganisms related to STI (Anyplex™ II STI-7 Detection which detects Neisseria gonorrhoeae, Chlamydia trachomatis, M...
2018: Journal of UOEH
https://www.readbyqxmd.com/read/29530863/-in-vitro-activity-of-lefamulin-against-sexually-transmitted-bacterial-pathogens
#19
Susanne Paukner, Astrid Gruss, Jørgen Skov Jensen
The pleuromutilin antibiotic lefamulin demonstrated in vitro activity against the most relevant bacterial pathogens causing sexually transmitted infections (STI), including Chlamydia trachomatis (MIC50/90 , 0.02/0.04 mg/liter; n = 15), susceptible and multidrug-resistant Mycoplasma genitalium (MIC range, 0.002 to 0.063 mg/liter; n = 6), and susceptible and resistant Neisseria gonorrhoeae (MIC50/90 , 0.12/0.5 mg/liter; n = 25). The results suggest that lefamulin could be a promising first-line antibiotic for the treatment of STI, particularly in populations with high rates of resistance to standard-of-care antibiotics...
May 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29509566/prevalence-of-mycoplasma-genitalium-and-azithromycin-resistant-infections-among-remnant-clinical-specimens-los-angeles
#20
Lao-Tzu Allan-Blitz, Elisa Mokany, Shelley Miller, Rachel Wee, Chelsea Shannon, Jeffrey D Klausner
BACKGROUND: Mycoplasma genitalium is an important cause of bacterial sexually transmitted diseases. Diagnosis and susceptibility testing of Mycoplasma genitalium are limited by the fastidious nature of the organism. Therefore, the prevalence of infection and azithromycin resistance are poorly studied. METHODS: We conducted an exploratory study on remnant clinical specimens. We collected remnant DNA from urine samples and clinical swabs (cervical/vaginal, rectal, and pharyngeal) previously tested for Neisseria gonorrhoeae and Chlamydia trachomatis using the Cobas 4800 CT/NG assay (Roche Molecular Systems, Pleasanton, CA) between March-April 2017 from across the University of California, Los Angeles Health System...
March 5, 2018: Sexually Transmitted Diseases
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