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streptococcus pyogenes

Amit Shivajirao Waghmare, Shivaji Sandu Pandit, Dayanand M Suryawanshi
AIM AND OBJECTIVE: 4H-pyran is one of the most well-known groups of synthetic heterocyclic compounds and it has attracted considerable attention of chemists in recent years because of their extensive range of biological and pharmaceutical activities. These compounds are used as antibacterial, anticancer agents, anti-coagulants, spasmolytics and anti-anaphylactic. 4H-pyran derivatives are utilized in cosmetics, pigments, biodegradable agrochemicals as well as photoactive materials. In addition, 4H-pyrans are also helpful as cognitive enhancers for the treatment of neuro degenerative diseases, including Alzheimer's disease, as well as for the treatment of schizophrenia and myoclonus...
March 14, 2018: Combinatorial Chemistry & High Throughput Screening
Kennedy Daniel Mwambete, Mathias Eulambius
OBJECTIVES: The study aimed to determine the prevalence of otitis media (OM)-associated bacterial flora of asymptomatic people living with HIV (PLH) on antiretroviral therapy (ART) and assess antibiotic resistance profiles of the bacteria. METHODOLOGY: Ear secretion specimens were collected by syringe or cotton swabs. Isolated bacteria were subjected to antibiotic sensitivity tests. RESULTS: Of 290 recruited PLH, 81.7% were females and 18...
January 2018: Journal of the International Association of Providers of AIDS Care
Maria Soledad Morales-Covarrubias, María Del Carmen Bolan-Mejía, Ana Isabel Vela Alonso, Jose F Fernandez-Garayzabal, Bruno Gomez-Gil
Strain CAIM 1838T , isolated from the hepatopancreas of a cultured diseased Pacific white shrimp (Penaeusvannamei), was subjected to characterization by a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence analysis, strain CAIM 1838T was most closely related to Streptococcus bovimastitidis 99.3 % and to other species of the Pyogenes clade of Streptococcus with lower similarity values. Average nucleotide identity values and the genome-to-genome distance of strain CAIM 1838T , as compared with the type strains, confirmed the separate species status with closely related species of the genus Streptococcus and were all below the thresholds to delimit a species, 93...
March 13, 2018: International Journal of Systematic and Evolutionary Microbiology
Anshu Babbar, Andreas Itzek, Dietmar H Pieper, D Patric Nitsche-Schmitz
Streptococcus dysgalactiae subsp. equisimilis (SDSE), belonging to the group C and G streptococci, are human pathogens reported to cause clinical manifestations similar to infections caused by Streptococcus pyogenes. To scrutinize the distribution of gene coding for S. pyogenes virulence factors in SDSE, 255 isolates were collected from humans infected with SDSE in Vellore, a region in southern India, with high incidence of SDSE infections. Initial evaluation indicated SDSE isolates comprising of 82.35% group G and 17...
March 12, 2018: Folia Microbiologica
Jacklyn R Hurst, Katherine J Kasper, Akshay N Sule, John K McCormick
Streptococcus pyogenes is a human-specific and globally prominent bacterial pathogen that despite causing numerous human infections, this bacterium is normally found in an asymptomatic carrier state. This review provides an overview of both bacterial and human factors that likely play an important role in nasopharyngeal colonization and pharyngitis, as well as the development of acute rheumatic fever and rheumatic heart disease. Here we highlight a recently described role for bacterial superantigens in promoting acute nasopharyngeal infection, and discuss how these immune system activating toxins could be crucial to initiate the autoimmune process in rheumatic heart disease...
March 9, 2018: Infection, Genetics and Evolution
Afsaneh Khani, Nicole Popp, Bernd Kreikemeyer, Nadja Patenge
Regulatory RNAs play important roles in the control of bacterial gene expression. In this study, we investigated gene expression regulation by a putative glycine riboswitch located in the 5'-untranslated region of a sodium:alanine symporter family (SAF) protein gene in the group A Streptococcus pyogenes serotype M49 strain 591. Glycine-dependent gene expression mediated by riboswitch activity was studied using a luciferase reporter gene system. Maximal reporter gene expression was observed in the absence of glycine and in the presence of low glycine concentrations...
2018: Frontiers in Microbiology
Kazuhiko Morii, Shigeyoshi Fujiwara, Shinichiro Nakamura, Hiroaki Okushin
No abstract text is available yet for this article.
March 9, 2018: Internal Medicine
Edmund Huang, Stanley C Jordan
No abstract text is available yet for this article.
March 9, 2018: Clinical Journal of the American Society of Nephrology: CJASN
Satish Maharaj, Karan Seegobin, Stephen Chrzanowski, Simone Chang
Streptococcus anginosus is a clinically important pathogen that is emerging globally but remains poorly investigated. Here, we report the first case of acute glomerulonephritis resulting from infection with S. anginosus Glomerulonephritis is typically caused by S. pyogenes and reports secondary to other strains including S. zooepidemicus and S. constellatus exist. Infection with S. anginosus in this patient was associated with acute nephritis (haematuria, oedema and hypertension), nephrotic syndrome and progressive azotemia...
March 9, 2018: BMJ Case Reports
Johannes Westman, Bhavya Chakrakodi, Johanna Snäll, Matthias Mörgelin, Martin Bruun Madsen, Ole Hyldegaard, Ariane Neumann, Inga-Maria Frick, Anna Norrby-Teglund, Lars Björck, Heiko Herwald
Innate immunity relies on an effective recognition of the pathogenic microorganism as well as on endogenous danger signals. While bacteria in concert with their secreted virulence factors can cause a number of inflammatory reactions, danger signals released at the site of infection may in addition determine the amplitude of such responses and influence the outcome of the disease. Here, we report that protein SIC, Streptococcal Inhibitor of Complement, an abundant secreted protein from Streptococcus pyogenes , binds to extracellular histones, a group of danger signals released during necrotizing tissue damage...
2018: Frontiers in Immunology
Mengyuan Hao, Yanhua Cui, Xiaojun Qu
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) loci, which provide a specific immunity against exogenous elements, are hypervariable among distinct prokaryotes. Based on previous researches, this review focuses on concluding systematical genome editing protocols in Streptococcus thermophilus. Firstly, its protocols and optimized conditions in gene editing are introduced. What's more, classification and diversity analyses of S. thermophilus CRISPR-Cas benefit the further understanding of evolution relationship among Streptococcus...
2018: Frontiers in Microbiology
Johnny H Hu, Shannon M Miller, Maarten H Geurts, Weixin Tang, Liwei Chen, Ning Sun, Christina M Zeina, Xue Gao, Holly A Rees, Zhi Lin, David R Liu
A key limitation to the use of CRISPR-Cas9 proteins for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from Streptococcus pyogenes (SpCas9), the required PAM sequence is NGG. No natural or engineered Cas9 variants shown to function efficiently in mammalian cells offer a PAM less restrictive than NGG. Here we used phage-assisted continuous evolution (PACE) to evolve an expanded PAM SpCas9 variant (xCas9) that can recognize a broad range of PAM sequences including NG, GAA, and GAT...
February 28, 2018: Nature
Nobuo Saito, Noriko Takamura, Grace P Retuerma, Carina H Frayco, Paul S Solano, Cherlyn D Ubas, Arianne V Lintag, Maricel R Ribo, Rontgene M Solante, Alexis Q Dimapilis, Elizabeth O Telan, Winston S Go, Motoi Suzuki, Koya Ariyoshi, Christopher M Parry
The widespread unregulated use of antibiotics without medical consultation contributes to the burden of antibiotic resistance in Southeast Asian countries. This study investigated antibiotic use before hospital consultation. In a prospective observational study from February 2, 2015, to July 2, 2015, we enrolled febrile patients attending the emergency room in San Lazaro Hospital, Manila, the Philippines. A urine sample was collected and a bioassay was used to detect antibiotic activity in urine using Bacillus stearothermophilus (ATCC7953), Escherichia coli (ATCC25922), and Streptococcus pyogenes (ATCC19615)...
March 5, 2018: American Journal of Tropical Medicine and Hygiene
Christofer A Q Karlsson, Sofia Järnum, Lena Winstedt, Christian Kjellman, Lars Bjorck, Adam Linder, Johan Malmström
Infectious diseases are characterized by a complex interplay between host and pathogen, but how these interactions impact the host proteome is unclear. Here we applied a combined mass spectrometry based proteomics strategy to investigate how the human proteome is transiently modified by the pathogen Streptococcus pyogenes , with a particular focus on bacterial cleavage of IgG in vivo In invasive diseases, S. pyogenes evokes a massive host response in blood, whereas superficial diseases are characterized by a local leakage of several blood plasma proteins at the site of infection including IgG...
March 6, 2018: Molecular & Cellular Proteomics: MCP
Aurélie Morand, Emmanouil Angelakis, Mehdi Ben Chaabane, Philippe Parola, Didier Raoult, Philippe Gautret
BACKGROUND: Skin infections are among the leading causes of diseases in travelers. Diagnosing pathogens could be difficult. METHOD: We applied molecular assays for the diagnostic of a large collection of skin biopsies and swabs from travelers with suspected skin infections. All samples were tested by qPCR for Coxiella burnetti, Bartonella sp., Rickettsia sp., Borrelia sp., Ehrlichia sp., Tropheryma whipplei, Francisella tularensis, Mycobacteria sp., Staphylococcus aureus, Streptococcus pyogenes, Leishmania spp...
March 2, 2018: Travel Medicine and Infectious Disease
Iktae Kim, Migyeong Jeong, Donghyun Ka, Mookyoung Han, Nak-Kyoon Kim, Euiyoung Bae, Jeong-Yong Suh
The bacterial CRISPR-Cas system provides adaptive immunity against invading phages. Cas9, an RNA-guided endonuclease, specifically cleaves target DNA substrates and constitutes a well-established platform for genome editing. Recently, anti-CRISPR (Acr) proteins that inhibit Cas9 have been discovered, promising a useful off-switch for Cas9 to avoid undesirable off-target effects. Here, we report the solution structure and dynamics of Listeria monocytogenes AcrIIA4 that inhibits Streptococcus pyogenes Cas9 (SpyCas9)...
March 1, 2018: Scientific Reports
Kun Cao, Nan Li, Hongcui Wang, Xin Cao, Jiaojiao He, Bing Zhang, Qing-Yu He, Gong Zhang, Xuesong Sun
Zinc is an essential metal in bacteria. One important bacterial zinc transporter is AdcA, and most bacteria possess AdcA homologs that are single-domain small proteins owing to better efficiency of protein biogenesis. However, a double-domain AdcA with two zinc-binding sites is significantly overrepresented in Streptococcus species, many of which are major human pathogens. Using molecular simulation and experimental validations of AdcA from Streptococcus pyogenes, we found here that the two AdcA domains sequentially stabilize the structure upon zinc binding, indicating an organization required for both increased zinc affinity and transfer speed...
February 28, 2018: Journal of Biological Chemistry
Nursenem Karaca, Betül Demirci, Fatih Demirci
Lavandula stoechas subsp. stoechas and Mentha spicata subsp. spicata are used for the treatment of sinusitis in Turkish folk medicine. The components of essential oils obtained by hydrodistillation were determined by gas chromatography-flame ionization detector (GC-FID), gas chromatography/mass spectrometry (GC/MS), and thin layer chromatography (TLC). Major components of L. stoechas and M. spicata oils were determined as camphor (46.7%) and carvone (60.6%), respectively. The antibacterial activity of essential oils and their main components were tested against the common selected sinusitis pathogens Streptococcus pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis, and Pseudomonas aeruginosa using in vitro agar diffusion, microdilution, and vapor diffusion methods...
February 27, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
OBJECTIVE: Previously, we developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology, which isolates specific genomic regions while preserving their molecular interactions. In enChIP, the locus of interest is tagged with engineered DNA-binding molecules such as the clustered regularly interspaced short palindromic repeats (CRISPR) system, consisting of a catalytically inactive form of Cas9 (dCas9) and guide RNA, followed by affinity purification of the tagged locus to allow identification of associated molecules...
February 27, 2018: BMC Research Notes
Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii
We developed the engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology to isolate specific genomic regions while retaining their molecular interactions. In enChIP, the locus of interest is tagged with an engineered DNA-binding molecule, such as a modified form of the clustered regularly interspaced short palindromic repeats (CRISPR) system containing a guide RNA (gRNA) and a catalytically inactive form of Cas9 (dCas9). The locus is then affinity-purified to enable identification of associated molecules...
February 26, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
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