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https://www.readbyqxmd.com/read/28944379/septic-arthritis-of-the-temporomandibular-joint-after-acute-otitis-media-in-a-child
#1
Kathia Dubron, Jan Meeus, Koenraad Grisar, Stefanie Desmet, Titiaan Dormaar, Yannick Spaey, Constantinus Politis
Septic arthritis of the temporomandibular joint as a complication of acute otitis media is rare in the Western world. This report describes the case of a 7-year-old boy who had pain in his right ear and limited mouth opening, following the onset of acute otitis media. A contrast-enhanced computed tomography scan revealed right-sided mastoiditis and hydrops of the right temporomandibular joint, suggesting septic arthritis. Real-time PCR and microbiologic analysis identified Streptococcus pyogenes and Staphylococcus epidermidis in the joint aspirate...
September 22, 2017: Quintessence International
https://www.readbyqxmd.com/read/28942759/binding-host-proteins-to-the-m-protein-contributes-to-the-mortality-associated-with-influenza-streptococcus-pyogenes-superinfections
#2
Andrea L Herrera, Kuta Suso, Stephanie Allison, Abby Simon, Evelyn Schlenker, Victor C Huber, Michael S Chaussee
The mortality associated with influenza A virus (IAV) is often due to the development of secondary bacterial infections known as superinfections. The group A streptococcus (GAS) is a relatively uncommon cause of IAV superinfections, but the mortality of these infections is high. We used a murine model to determine whether the surface-localized GAS M protein contributes to the outcome of IAV-GAS superinfections. A comparison between wild-type GAS and an M protein mutant strain (emm3) showed that the M3 protein was essential to virulence...
September 25, 2017: Microbiology
https://www.readbyqxmd.com/read/28942408/asymptomatic-pyogenic-liver-abscesses-secondary-to-fusobacterium-nucleatum-and-streptococcus-vestibularis-in-an-immunocompetent-patient
#3
Hamish Houston, Kartik Kumar, Salman Sajid
A 66-year-old male patient presented with symptoms and signs of L4/5 radiculopathy. He was found to be anaemic with elevated inflammatory markers and deranged hepatic enzymes. Imaging revealed lumbar canal stenosis and the presence of pyogenic liver abscesses from which Fusobacterium nucleatum and Streptococcus vestibularis were isolated. The hepatic abscesses were attributed to asymptomatic diverticular perforation. Multiple coexisting incidental infections were discovered, including oesophageal candidiasis, Helicobacter pylori, stool cultures positive for Strongyloides stercoralis, and sputum cultures positive for Enterobacter cloacae, Escherichia coli and Mycobacterium avium Extensive investigations for possible underlying immunosuppression were negative...
September 23, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28939394/enzyme-linked-immunosorbent-assay-for-group-a-streptococcal-anti-dnase-b-in-human-sera-using-recombinant-proteins-comparison-to-the-dna-methyl-green-micromethod
#4
Sarita Das, T Dileepan, D R Johnson, E L Kaplan, P Patrick Cleary
Among the four known Streptococcal nucleases comprising of DNase A, B, C and D; DNase B is the most common, and determination of the levels of antibody to DNase B (ADB) is often used to confirm a clinical diagnosis of Streptococcus pyogenes/group A Streptococcal (GAS) infection. The commonly used assays for antibodies that neutralize DNase B or streptolysin O activity use partially purified antigens that often fail to detect antibody changes subsequent to culture documented infections. Therefore, an enzyme-linked immunosorbent assay (ELISA) was developed employing his-tagged recombinant DNase B as plate antigen for comparison to the commonly used DNA methyl green micromethod (DMGM)...
September 19, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28936671/use-of-the-cas9-orthologs-from-streptococcus-thermophilus-and-staphylococcus-aureus-for-non-homologous-end-joining-mediated-site-specific-mutagenesis-in-arabidopsis-thaliana
#5
Jeannette Steinert, Carla Schmidt, Holger Puchta
Since the discovery of the CRISPR/Cas system and its in vivo application for site-specific targeted mutagenesis, this technique is wildly used in a great variety of organisms, such as many plant species. Commonly used for this application is the Cas9 enzyme from Streptococcus pyogenes. Here, we describe the application of two Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for targeted non-homologous end-joining mediated mutagenesis in Arabidopsis thaliana. With both orthologs, we could show efficient inheritance of the induced mutations...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28935732/complete-genome-sequences-of-emm111-type-streptococcus-pyogenes-strain-gur-with-antitumor-activity-and-its-derivative-strain-gursa1-with-an-inactivated-emm-gene
#6
Maria A Suvorova, Anna N Tsapieva, Emilie Glad Bak, Valery A Chereshnev, Ekaterina P Kiseleva, Alexander N Suvorov, Manimozhiyan Arumugam
We present here the complete genome sequence of Streptococcus pyogenes type emm111 strain GUR, a throat isolate from a scarlet fever patient, which has been used to treat cancer patients in the former Soviet Union. We also present the complete genome sequence of its derivative strain GURSA1 with an inactivated emm gene.
September 21, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28933370/a-case-of-systemic-infection-caused-by-streptococcus-pyogenes-oral-infection-in-an-edentulous-patient
#7
Yumi Inagaki, Masanobu Abe, Ryoko Inaki, Liang Zong, Hideyuki Suenaga, Takahiro Abe, Kazuto Hoshi
BACKGROUND: Infections in the oral and maxillofacial region can sometimes extend beyond the oral cavity, with serious consequences. Most oral infections are odontogenic, occurring through the root apex of the tooth or the periodontal pocket. It thus makes sense that edentulous patients have a much lower risk of oral bacterial infection. For this reason, while there are many reports on systemic infections caused by oral infections, few of these describe such infections in edentulous patients...
August 18, 2017: Diseases (Basel)
https://www.readbyqxmd.com/read/28931002/enhanced-proofreading-governs-crispr-cas9-targeting-accuracy
#8
Janice S Chen, Yavuz S Dagdas, Benjamin P Kleinstiver, Moira M Welch, Alexander A Sousa, Lucas B Harrington, Samuel H Sternberg, J Keith Joung, Ahmet Yildiz, Jennifer A Doudna
The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing(1-4). High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discrimination and the potential to further improve fidelity are unknown(5-9). Using single-molecule Förster resonance energy transfer (smFRET) experiments, we show that both SpCas9-HF1 and eSpCas9(1.1) are trapped in an inactive state(10) when bound to mismatched targets...
September 20, 2017: Nature
https://www.readbyqxmd.com/read/28930240/anti-bacterial-activity-of-phenolic-compounds-against-streptococcus-pyogenes
#9
Sabrina Macé, Lisbeth Truelstrup Hansen, H P Vasantha Rupasinghe
BACKGROUND: Worldwide, Streptococcus pyogenes is the leading cause of bacterial pharyngitis. To reduce the use of antibiotics, antimicrobial phytochemical-containing remedies, which have long been in use in traditional medicine, may provide new approaches for management of streptococcal pharyngitis. The objective of this study was to assess the inhibitory activities of 25 natural phenolic compounds against three strains of S. pyogenes. METHODS: After an initial screening, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the nine most effective phenolic compounds were determined...
May 1, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28928212/population-and-whole-genome-sequence-based-characterization-of-invasive-group-a-streptococci-recovered-in-the-united-states-during-2015
#10
Sopio Chochua, Ben J Metcalf, Zhongya Li, Joy Rivers, Saundra Mathis, Delois Jackson, Robert E Gertz, Velusamy Srinivasan, Ruth Lynfield, Chris Van Beneden, Lesley McGee, Bernard Beall
Group A streptococci (GAS) are genetically diverse. Determination of strain features can reveal associations with disease and resistance and assist in vaccine formulation. We employed whole-genome sequence (WGS)-based characterization of 1,454 invasive GAS isolates recovered in 2015 by Active Bacterial Core Surveillance and performed conventional antimicrobial susceptibility testing. Predictions were made for genotype, GAS carbohydrate, antimicrobial resistance, surface proteins (M family, fibronectin binding, T, R28), secreted virulence proteins (Sda1, Sic, exotoxins), hyaluronate capsule, and an upregulated nga operon (encodes NADase and streptolysin O) promoter (Pnga3)...
September 19, 2017: MBio
https://www.readbyqxmd.com/read/28926145/a-crispr-reimagining-new-twists-and-turns-of-crispr-beyond-the-genome-engineering-revolution
#11
Robert J Plummer, Yi Guo, Ying Peng
Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting "off-label" applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28922066/the-effect-of-lysozyme-on-reducing-biofilms-by-staphylococcus-aureus-pseudomonas-aeruginosa-and-gardnerella-vaginalis-an-in-vitro-examination
#12
Mirsada Hukić, Dzenita Seljmo, Amra Ramovic, Monia Avdić Ibrišimović, Serkan Dogan, Jasna Hukic, Elma Feric Bojic
Two basic questions about lysozyme activities on the selected microorganisms were investigated, namely whether lysozyme inhibits biofilm production and which concentrations of the enzyme have the ability to change the natural biofilm producing capacity of different strains of Staphylococcus aureus (methicillin sensitive and resistant), Streptococcus pyogenes, Pseudomonas aeruginosa, and Gardnerella vaginalis. The effect of lysozyme on biofilm formation capacities of 16 strains of selected microorganisms was investigated, whereby four testing replicates have been performed in vitro using the Test Tube method, and the potential of lysozyme to change biofilm forming capacities depending on its concentration, species, and strains of microorganisms is demonstrated...
September 18, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28919415/biophysical-characterization-of-the-interaction-between-heme-and-proteins-responsible-for-heme-transfer-in-streptococcus-pyogenes
#13
Masato Hoshino, Makoto Nakakido, Satoru Nagatoishi, Chihiro Aikawa, Ichiro Nakagawa, Kouhei Tsumoto
Streptococcus pyogenes, an important pathogen that causes a wide range of diseases, possesses the sia gene cluster, which encodes proteins involved in the heme acquisition system. Although this system was previously described, the molecular mechanism of effective heme transfer remains to be elucidated. Here, we have characterized the interactions between heme and each domain of Streptococcal hemoprotein receptor (Shr) and Streptococcal heme-binding protein (Shp). Our kinetic and thermodynamic analyses suggested that effective heme transfer within this system is achieved not only by affinity-based transfer but also by the difference of the binding driving force...
September 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28919333/status-of-the-effectiveness-of-contact-lens-disinfectants-in-malaysia-against-keratitis-causing-pathogens
#14
Farhat Abjani, Naveed Ahmed Khan, Suk Yul Jung, Ruqaiyyah Siddiqui
The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept(®), AO SEPT PLUS, OPTI-FREE(®) pure moist(®), Renu(®) fresh™, FreshKon(®) CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT)...
September 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28917068/group-a-streptococcal-bacteraemia-experience-at-king-fahad-medical-city-in-riyadh-saudi-arabia
#15
Fawzyh J Al-Khadidi, Mohammed A AlSheheri, Tariq S AlFawaz, Mushira A Enani, Abdulaziz A AlAqeel, Dayel A AlShahrani
To identify clinical presentation, predisposing factors, and the outcome in patients with Group A Streptococcal bacteremia. Methods: This is a retrospective study of 33 pediatric and adult patients with Streptococcus pyogenes bacteremia, admitted at King Fahad Medical City, Riyadh, Kingdom of Saudi Arabia from 2007 to 2015. Results: Thirty-three patient records, documenting bacteremia with group A beta-hemolytic streptococcus, were reviewed. Approximately 51.5% were pediatric and 48.5% were adult patients, with a male to female ratio of 2:1...
October 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28916372/tracking-and-controlling-soft-surface-contamination-in-health-care-settings
#16
Jonathan D Sexton, Amanda M Wilson, Hannah P Sassi, Kelly A Reynolds
BACKGROUND: Study objectives were to track the transfer of microbes on soft surfaces in health care environments and determine the efficiency of an Environmental Protection Agency (EPA)-registered soft surface sanitizer in the health care environment. METHODS: Soft surfaces at 3 health care facilities were sampled for heterotrophic plate count (HPC) bacteria, Staphylococcus spp, Streptococcus pyogenes, and Escherichia coli followed by a tracer study with a virus surrogate seeded onto volunteer hands and commonly touched surfaces...
September 12, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28904125/enhancing-vaccine-efficacy-by-engineering-a-complex-synthetic-peptide-to-become-a-super-immunogen
#17
Therése Nordström, Manisha Pandey, Ainslie Calcutt, Jessica Powell, Zachary N Phillips, Grace Yeung, Ashwini K Giddam, Yun Shi, Thomas Haselhorst, Mark von Itzstein, Michael R Batzloff, Michael F Good
Peptides offer enormous promise as vaccines to prevent and protect against many infectious and noninfectious diseases. However, to date, limited vaccine efficacy has been reported and none have been licensed for human use. Innovative ways to enhance their immunogenicity are being tested, but rational sequence modification as a means to improve immune responsiveness has been neglected. Our objective was to establish a two-step generic protocol to modify defined amino acids of a helical peptide epitope to create a superior immunogen...
September 13, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28902290/effect-of-secondary-infection-on-epithelialisation-and-total-healing-of-cutaneous-leishmaniasis-lesions
#18
Liliane de Fátima Antonio, Marcelo Rosandiski Lyra, Maurício Naoto Saheki, Armando de Oliveira Schubach, Luciana de Freitas Campos Miranda, Maria de Fátima Madeira, Maria Cristina da Silva Lourenço, Aline Fagundes, Érica Aparecida Dos Santos Ribeiro, Leonardo Barreto, Maria Inês Fernandes Pimentel
BACKGROUND: Cutaneous leishmaniasis (CL) generally presents with a single or several localised cutaneous ulcers without involvement of mucous membranes. Ulcerated lesions are susceptible to secondary contamination that may slow the healing process. OBJECTIVE: This study verified the influence of non-parasitic wound infection on wound closure (epithelialisation) and total healing. METHODS: Twenty-five patients with a confirmed diagnosis of CL and ulcerated lesions underwent biopsy of ulcer borders...
September 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28887213/bivalent-mucosal-peptide-vaccines-administered-using-the-lcp-carrier-system-stimulate-protective-immune-responses-against-streptococcus-pyogenes-infection
#19
Kai Schulze, Thomas Ebensen, Saranya Chandrudu, Mariusz Skwarczynski, Istvan Toth, Colleen Olive, Carlos A Guzman
Despite the broad knowledge about the pathogenicity of Streptococcus pyogenes there is still a controversy about the correlate of protection in GAS infections. We aimed in further improving the immune responses stimulated against GAS comparing different vaccine formulations including bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and BPPCysMPEG, a derivative of the macrophage-activating lipopeptide (MALP-2), as adjuvants, respectively, to be administered with and without the universal T helper cell epitope P25 along with the optimized B cell epitope J14 of the M protein and B and T cell epitopes of SfbI...
September 5, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28882851/managing-scarlet-fever
#20
(no author information available yet)
Scarlet fever, an infection caused by toxin-producing strains of Streptococcus pyogenes, was associated with high levels of morbidity and mortality when epidemics were common in the 18(th) and 19(th) centuries throughout Europe and the USA.(1) Although this disease nearly disappeared during the 20(th) century, several countries, including the UK, have recently experienced a re-emergence of scarlet fever.(1-3) However, the reason for these new outbreaks remains unclear.(1,4) Despite a general move to reduce the use of antibiotics for many mild self-limiting infections (e...
September 2017: Drug and Therapeutics Bulletin
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