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streptococcus pyogenes

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https://www.readbyqxmd.com/read/28429163/antimicrobial-susceptibility-of-bacterial-isolates-from-patients-presenting-with-ear-nose-and-throat-ent-infections-in-the-german-community-healthcare-setting
#1
B Olzowy, M Kresken, M Havel, D Hafner, B Körber-Irrgang
Empiric initial antibiotic therapy of bacterial infections is based primarily upon the susceptibility of the most common causative pathogens. The purpose of this study was to provide susceptibility data on six bacterial species known to cause ear, nose and throat (ENT) infections. A total of 1066 isolates collected during a nationwide laboratory-based surveillance study were analysed. All Streptococcus pyogenes isolates were penicillin (PEN)-susceptible, indicating that natural penicillins can still be recommended as the first-line treatment for group A streptococcal tonsillopharyngitis...
April 20, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28427277/biological-activity-and-lc-ms-ms-profiling-of-extracts-from-the-australian-medicinal-plant-acacia-ligulata-fabaceae
#2
Diana Jæger, Bradley S Simpson, Chi P Ndi, Anna K Jäger, Christoph Crocoll, Birger Lindberg Møller, Philip Weinstein, Susan J Semple
Acacia ligulata A.Cunn. ex Benth. (Fabaceae: Mimosoideae) is a native Australian plant used traditionally by Australian Aboriginal groups. This study was undertaken to investigate the bioactivity of A. ligulata extracts and to evaluate their chemical composition. Potential antibacterial, cytotoxic and enzyme inhibitory effects relevant to traditional medicinal and food uses of the species were examined and LC-MS/MS was performed to investigate the chemical composition. Antibacterial activity was observed for bark and leaf extracts with an MIC for the bark extract of 62...
April 21, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28414746/local-th17-iga-immunity-correlate-with-protection-against-intranasal-infection-with-streptococcus-pyogenes
#3
Rasmus Mortensen, Dennis Christensen, Lasse Bøllehuus Hansen, Jan Pravsgaard Christensen, Peter Andersen, Jes Dietrich
Streptococcus pyogenes (group A streptococcus, GAS) is responsible for a wide array of infections. Respiratory transmission via droplets is the most common mode of transmission but it may also infect the host via other routes such as lesions in the skin. To advance the development of a future vaccine against GAS, it is therefore important to investigate how protective immunity is related to the route of vaccine administration. To explore this, we examined whether a parenterally administered anti-GAS vaccine could protect against an intranasal GAS infection or if this would require locally primed immunity...
2017: PloS One
https://www.readbyqxmd.com/read/28411216/construction-of-a-dcas9-based-gene-knockdown-system-in-staphylococcus-aureus
#4
Changlong Zhao, Xueqin Shu, Baolin Sun
There has been constant absence of an efficient gene knockdown method in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The early developed antisense RNA technology is mainly applied for forward genetic screen, but is rather limiting in specific gene knockdown for the lack of rational antisense RNA design strategies. Here we report an efficient and specific gene knockdown system in S. aureus based on type II clustered regularly interspaced short palindromic repeats (CRISPR) system from Streptococcus pyogenes...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28401320/the-virulence-factors-of-group-a-streptococcus-strains-isolated-from-invasive-and-non-invasive-infections-in-polish-and-german-centres-2009-2011
#5
M Strus, P B Heczko, E Golińska, A Tomusiak, A Chmielarczyk, M Dorycka, M van der Linden, A Samet, A Piórkowska
Streptococcus pyogenes (GAS) is one of the major human pathogenic bacteria that cause a wide range of diseases. Currently, increased incidence of streptococcal invasive infections is observed worldwide. In this study, we focused on the prevalence of genes encoding superantigens and type M proteins in the population of GAS strains from invasive versus non-invasive infections. We tested 253 GAS strains: 48 strains from patients with invasive infections (18 from wound/deep skin localization, 30 from women in labour) and 205 strains from non-invasive forms (147 from common infections of the upper respiratory, 49 from the vagina of females with genital tract infections and 9 from non-invasive wound and superficial skin infections)...
April 11, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28401063/scm-the-m-protein-of-streptococcus-canis-binds-immunoglobulin-g
#6
Simone Bergmann, Inga Eichhorn, Thomas P Kohler, Sven Hammerschmidt, Oliver Goldmann, Manfred Rohde, Marcus Fulde
The M protein of Streptococcus canis (SCM) is a virulence factor and serves as a surface-associated receptor with a particular affinity for mini-plasminogen, a cleavage product of the broad-spectrum serine protease plasmin. Here, we report that SCM has an additional high-affinity immunoglobulin G (IgG) binding activity. The ability of a particular S. canis isolate to bind to IgG significantly correlates with a scm-positive phenotype, suggesting a dominant role of SCM as an IgG receptor. Subsequent heterologous expression of SCM in non-IgG binding S...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28397552/in-vitro-antimicrobial-activity-of-thymus-vulgaris-essential-oil-against-major-oral-pathogens
#7
Mohammadmehdi Fani, Jamshid Kohanteb
The objective of present investigation was to determine antimicrobial activity of Thymus vulgaris oil on some oral pathogens. Thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of Streptococcus pyogenes, Streptococcus mutans, Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. Thymus vulgaris oil at concentrations of 16 to 256 μg/mL exhibited strong inhibitory activity on all clinical isolates producing inhibition zones of 7...
January 1, 2017: Journal of Evidence-based Complementary & Alternative Medicine
https://www.readbyqxmd.com/read/28375596/efficient-transcriptional-gene-repression-by-type-v-a-crispr-cpf1-from-eubacterium-eligens
#8
Seong Keun Kim, Haseong Kim, Woo-Chan Ahn, Kwang-Hyun Park, Eui-Jeon Woo, Dae-Hee Lee, Seung-Goo Lee
Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is an emerging technology for artificial gene regulation. Type II CRISPR-Cas endonuclease Cas9 is the most widely used protein for gene regulation with CRISPRi. Here, we present type V-A CRISPR-Cas endonuclease Cpf1-based CRISPRi. We constructed an l-rhamnose-inducible CRISPRi system with DNase-deactivated Cpf1 from Eubacterium eligens (EedCpf1) and compared its performance with catalytically deactivated Cas9 from Streptococcus pyogenes (SpdCas9)...
April 11, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28373352/the-perr-regulated-p1b-4-type-atpase-pmta-acts-as-a-ferrous-iron-efflux-pump-in-streptococcus-pyogenes
#9
Andrew G Turner, Cheryl-Lynn Y Ong, Karrera Y Djoko, Nicholas P West, Mark R Davies, Alastair G McEwan, Mark J Walker
Streptococcus pyogenes (group A Streptococcus; GAS) is an obligate human pathogen responsible for a broad spectrum of human disease. GAS has a requirement for metal homeostasis within the human host and as such, tightly modulates metal uptake and efflux during infection. Metal acquisition systems are required to combat metal sequestration by the host, while metal efflux systems are essential to protect against metal overload poisoning. Here, we investigated the function of PmtA (PerR-regulated metal transporter A), a P1B-4 type ATPase efflux pump, in the invasive GAS M1T1 strain 5448...
April 3, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28372355/throat-carriage-rate-and-antimicrobial-resistance-of-streptococcus-pyogenes-in-rural-children-in-argentina
#10
Gastón Delpech, Mónica Sparo, Beatriz Baldaccini, Gisela Pourcel, Sabina Lissarrague, Leonardo García Allende
OBJECTIVES: The aim of this study was to determine the prevalence of asymptomatic carriers of group A β-hemolytic streptococci (GAS) in children living in a rural community and to investigate the association between episodes of acute pharyngitis and carrier status. METHODS: Throat swabs were collected from September to November 2013 among children 5-13 years of age from a rural community (Maria Ignacia-Vela, Argentina). The phenotypic characterization of isolates was performed by conventional tests...
March 2017: Journal of Preventive Medicine and Public Health, Yebang Ŭihakhoe Chi
https://www.readbyqxmd.com/read/28371831/a-split-staphylococcus-aureus-cas9-as-a-compact-genome-editing-tool-in-plants
#11
Hidetaka Kaya, Kazuhiro Ishibashi, Seiichi Toki
Split-protein methods-where a protein is split into two inactive fragments that must re-assemble to form an active protein-can be used to regulate the activity of a given protein and reduce the size of gene transcription units. Here, we show that a Staphylococcus aureus Cas9 (SaCas9) can be split, and that split-SaCas9 expressed from Agrobacterium can induce targeted mutagenesis in Nicotiana benthamiana. Since SaCas9 is smaller than the more commonly used Cas9 derived from Streptococcus pyogenes, the split-SaCas9 provides the smallest tool yet for clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) plant genome editing...
April 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28371435/interference-of-a-speb-5-utr-partial-deletion-with-mrna-degradation-in-streptococcus-pyogenes
#12
Zhiyun Chen, Lauren Mashburn-Warren, Justin Merritt, Michael J Federle, Jens Kreth
The 5' untranslated region (5' UTR) of an mRNA molecule embeds important determinants that modify its stability and translation efficiency. In Streptococcus pyogenes, a strict human pathogen, a gene encoding a secreted protease (speB) has a large 5' UTR with unknown functions. Here we describe that a partial deletion of the speB 5' UTR caused a general accumulation of mRNA in the stationary phase, and that the mRNA accumulation was due to retarded mRNA degradation. The phenotype was observed in several M serotypes harboring the partial deletion of the speB 5' UTR...
March 30, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28371222/new-variants-of-crispr-rna-guided-genome-editing-enzymes
#13
REVIEW
Jana Murovec, Žan Pirc, Bing Yang
CRISPR-mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is revolutionizing life science by providing new, precise, facile and high throughput tools for genetic modification by the specific targeting of double-strand breaks in the genome of hosts. Plant biotechnologists have extensively used the S. pyogenes Cas9-based system since its inception in 2013. However, there are still some limitations to its even broader usage in plants. Major restrictions, especially in agricultural biotechnology, are the currently unclear regulatory status of plants modified with CRISPR/Cas9 and the lack of suitable delivery methods for some plant species...
April 1, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28370366/new-insights-on-the-antibacterial-efficacy-of-miconazole-in-vitro
#14
P Nenoff, D Koch, C Krüger, C Drechsel, P Mayser
Miconazole is a broad-spectrum antifungal used in topical preparations. In the present investigation the minimal inhibitory concentration (MIC) of miconazole for eighty wild type strains of gram-positive and gram-negative bacteria isolated from infected skin lesions was assessed using a modified agar dilution test (adapted to CLSI, Clinical Laboratory Standards Institute). 14 ATCC reference strains served as controls. Miconazole was found efficacious against gram-positive aerobic bacteria (n=62 species), the MICs against Staphylococcus (S...
March 30, 2017: Mycoses
https://www.readbyqxmd.com/read/28369500/order-of-stability-for-proteolysis-sites-of-a-bacterial-collagen-like-protein
#15
Liang Ma, Yalin Chai, Ting Wu, Mengyuan Wang
Compared to collagens isolated from animal sources, collagens and collagen-like proteins from non-animal sources are non-immunogenic and thus promising as biomedical materials. Recently, a collagen-like protein, V-CL, was identified from a bacterial source, Streptococcus pyogenes. In this study, an acid-precipitation method was used to isolate V-CL in one purification step. Circular dichroism spectroscopy was used to examine the triple-helix structure of V-CL. The trypsin proteolysis events of V-CL were characterized using gel electrophoresis and mass spectrometry...
March 23, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28366695/-streptococcus-pyogenes-infection-in-paediatrics-from-pharyngotonsillitis-to-invasive-infections
#16
David Espadas Maciá, Eva María Flor Macián, Rafael Borrás, Sandrine Poujois Gisbert, Juan Ignacio Muñoz Bonet
BACKGROUND: Streptococcus pyogenes or Group A Streptococci (GAS) cause many infections in infancy. Changes in its epidemiology have been described in recent years, including an increase in invasive infections (iGAS). METHODS: A retrospective-descriptive study was conducted on children less than 15 years old, with GAS infections, in particular iGAS, and their complications from February 2004-April 2014. RESULTS: A total of 2,192 positive cultures were obtained of which 92...
March 31, 2017: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
https://www.readbyqxmd.com/read/28361960/sequence-analysis-of-pooled-bacterial-samples-enables-identification-of-strain-variation-in-group-a-streptococcus
#17
Rigbe G Weldatsadik, Jingwen Wang, Kai Puhakainen, Hong Jiao, Jari Jalava, Kati Räisänen, Neeta Datta, Tiina Skoog, Jaana Vuopio, T Sakari Jokiranta, Juha Kere
Knowledge of the genomic variation among different strains of a pathogenic microbial species can help in selecting optimal candidates for diagnostic assays and vaccine development. Pooled sequencing (Pool-seq) is a cost effective approach for population level genetic studies that require large numbers of samples such as various strains of a microbe. To test the use of Pool-seq in identifying variation, we pooled DNA of 100 Streptococcus pyogenes strains of different emm types in two pools, each containing 50 strains...
March 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28355251/immunization-with-a-streptococcal-multiple-epitope-recombinant-protein-protects-mice-against-invasive-group-a-streptococcal-infection
#18
Chih-Feng Kuo, Nina Tsao, I-Chen Hsieh, Yee-Shin Lin, Jiunn-Jong Wu, Yu-Ting Hung
Streptococcus pyogenes (group A Streptococcus; GAS) causes clinical diseases, including pharyngitis, scarlet fever, impetigo, necrotizing fasciitis and streptococcal toxic shock syndrome. A number of group A streptococcus vaccine candidates have been developed, but only one 26-valent recombinant M protein vaccine has entered clinical trials. Differing from the design of a 26-valent recombinant M protein vaccine, we provide here a vaccination using the polyvalence epitope recombinant FSBM protein (rFSBM), which contains four different epitopes, including the fibronectin-binding repeats domain of streptococcal fibronectin binding protein Sfb1, the C-terminal immunogenic segment of streptolysin S, the C3-binding motif of streptococcal pyrogenic exotoxin B, and the C-terminal conserved segment of M protein...
2017: PloS One
https://www.readbyqxmd.com/read/28351920/spxa1-and-spxa2-act-coordinately-to-fine-tune-stress-responses-and-virulence-in-streptococcus-pyogenes
#19
Gary C Port, Zachary T Cusumano, Paul R Tumminello, Michael G Caparon
SpxA is a unique transcriptional regulator highly conserved among members of the phylum Firmicutes that binds RNA polymerase and can act as an antiactivator. Why some Firmicutes members have two highly similar SpxA paralogs is not understood. Here, we show that the SpxA paralogs of the pathogen Streptococcus pyogenes, SpxA1 and SpxA2, act coordinately to regulate virulence by fine-tuning toxin expression and stress resistance. Construction and analysis of mutants revealed that SpxA1(-) mutants were defective for growth under aerobic conditions, while SpxA2(-) mutants had severely attenuated responses to multiple stresses, including thermal and oxidative stresses...
March 28, 2017: MBio
https://www.readbyqxmd.com/read/28348798/primary-pyogenic-ventriculitis-caused-by-neisseria-meningitidis-case-report-and-review-of-the-literature
#20
Firza Gronthoud, Ibrahim Hassan, Pippa Newton
Background. Pyogenic ventriculitis is a well-known complication of meningitis, brain abscesses and intraventricular drains. Primary pyogenic ventriculitis is a rare entity and few cases have been described so far. We report the first case of primary pyogenic ventriculitis in an adult caused by Neisseria meningitidis and present an overview of all reported adult primary pyogenic ventriculitis cases in the English literature. Methods. A PubMed search was performed using the terms ependymitis, ventricular empyema, pyocephalus and ventriculitis...
January 2017: JMM Case Reports
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