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https://www.readbyqxmd.com/read/28544962/effect-of-immobilization-on-the-antimicrobial-activity-of-a-cysteine-terminated-antimicrobial-peptide-cecropin-p1-tethered-to-silica-nanoparticle-against-e-coli-o157-h7-edl933
#1
Xi Wu, Pei-Hsun Wei, Xiao Zhu, Mary J Wirth, Arun Bhunia, Ganesan Narsimhan
Antimicrobial peptides (AMPs) have the ability to penetrate the cell membrane, form pores which eventually lead to cell death. Immobilization of AMP on nanoparticles can play a major role in antimicrobial materials, biosensors for pathogen detection and in food safety. The minimum inhibitory concentration (MIC) of free Cecropin P1 (CP1, sequence SWLSTAKKLENSAKKRLSEGIAIAIQGGPR) and adsorbed on silica nanoparticle against E. coli O157:H7 EDL933 were 0.78μg/ml. This was found to be consistent with preservation of α-helical secondary structure of CP1 upon adsorption as indicated by circular dichroism (CD)...
May 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544922/efficacy-of-benzalkonium-chloride-against-bioluminescent-p-aeruginosa-atcc9027-constructs
#2
N Shah, D C Naseby
Whole cell biosensors have been seldom used in the pharmaceutical and cosmetics industries for preservative efficacy testing (PET). According to several pharmacopoeias, preservatives should be tested for microbial activity using traditional viable count techniques; the use of whole cell microbial biosensors potentially provides an alternative, fast, and efficient method. The aim of the study was to assess the applicability of Pseudomonas aeruginosa ATCC9027 and its validated bioluminescent strains for preservative efficacy tests using benzalkonium chloride (BKC)...
April 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28544611/detection-of-nitric-oxide-from-living-cells-using-polymeric-zinc-organic-framework-derived-zinc-oxide-composite-with-conducting-polymer
#3
Min-Yeong Kim, Malenahalli Halappa Naveen, Nanjanagudu Ganesh Gurudatt, Yoon-Bo Shim
Sensitive and selective detection of nitric oxide (NO) in the human body is crucial since it has the vital roles in the physiological and pathological processes. This study reports a new type of electrochemical NO biosensor based on zinc-dithiooxamide framework derived porous ZnO nanoparticles and polyterthiophene-rGO composite. By taking advantage of the synergetic effect between ZnO and poly(TTBA-rGO) (TTBA = 3'-(p-benzoic acid)-2,2':5',2″-terthiophene, rGO = reduced graphene oxide) nanocomposite layer, the poly(TTBA-rGO)/ZnO sensor probe displays excellent electrocatalytic activity and explores to detect NO released from normal and cancer cell lines...
May 22, 2017: Small
https://www.readbyqxmd.com/read/28543037/naringenin-responsive-riboswitch-based-fluorescent-biosensor-module-for-escherichia-coli-co-cultures
#4
Yu Xiu, Sungho Jang, J Andrew Jones, Nicholas A Zill, Robert J Linhardt, Qipeng Yuan, Gyoo Yeol Jung, Mattheos A G Koffas
The ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high-throughput rapid screening techniques is of upmost importance for the future of synthetic biology and metabolic engineering. Here we describe the development of a RNA riboswitch-based biosensor module with dual fluorescent reporters, and demonstrate a high-throughput flow cytometry-based screening method for identification of naringenin over producing Escherichia coli strains in co-culture...
May 20, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28542806/structural-and-functional-study-of-a-simple-rapid-and-label-free-dnazyme-based-dna-biosensor-for-optimization-activity
#5
Narges Shahbazi, Saman Hosseinkhani, Khosro Khajeh, Bijan Ranjbar
Peroxidase-mimicking DNAzyme has a potential to self-assemble into a G-quadruplex and shows peroxidase activity. In comparison to proteins, peroxidase-mimicking DNAzyme is less expensive and more stable. Herein, it is used in fabricating non-labeling biosensors. This paper investigates the structural and functional properties of a DNA biosensor based on split DNAzyme with a detection limit in nM range (9.48 nM). Two halves of DNAzyme were linked by a complementary sequence of DNA target. Hybridization of the DNA target pulled two DNAzyme halves apart and peroxidase activity decreased...
May 20, 2017: Biopolymers
https://www.readbyqxmd.com/read/28542141/an-enhanced-bioluminescence-based-annexin-v-probe-for-apoptosis-detection-in-vitro-and-in-vivo
#6
Trajen Head, Peter Dau, Stephanie Duffort, Pirouz Daftarian, Pratibha M Joshi, Roberto Vazquez-Padron, Sapna K Deo, Sylvia Daunert
The process of controlled cellular death known as apoptosis has an important central role not only in normal homeostatic maintenance of tissues, but also in numerous diseases such as cancer, neurodegenerative, autoimmune, and cardiovascular diseases. As a result, new technologies with the capability to selectively detect apoptotic cells represent a central focus of research for the study of these conditions. We have developed a new biosensor for the detection of apoptotic cells, incorporating the targeted selectivity for apoptotic cells from Annexin V with the sensitivity of bioluminescence signal generation from a serum-stable mutant of Renilla luciferase (RLuc8)...
May 25, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28541225/stability-of-enzymatic-biosensors-for-wearable-applications
#7
Apurva Sonawane, Pandiaraj Manickam, Shekhar Bhansali
Technological evolution in wearable sensors is accounting for major growth and transformation in multitude of industries ranging from healthcare to computing & informatics to communication and biomedical sciences. The major driver for this transformation is the new-found ability to continuously monitor and analyze the patients' physiology in patients' natural setting. Numerous wearable sensors are already on the market and are summarized. Most of the current technologies have focused on electro-physiological, electro-mechanical or acoustic measurements...
May 19, 2017: IEEE Reviews in Biomedical Engineering
https://www.readbyqxmd.com/read/28540582/novel-synthesis-and-characterization-of-pristine-cu-nanoparticles-for-the-non-enzymatic-glucose-biosensor
#8
T Dayakar, K Venkateswara Rao, K Bikshalu, V Rajendar, Si-Hyun Park
Non enzymatic electrochemical glucose sensing was developed based on pristine Cu Nanopartilces (NPs)/Glassy Carbon Electrode (GCE) which can be accomplished by simple green method via ocimum tenuiflorum leaf extract. Then, the affect of leaf extract addition on improving Structural, Optical and electrochemical properties of pristine cu NPs was investigated. The synthesized Cu NPs were characterized with X-ray diffraction (X-ray), Uv-Visible spectroscopy (Uv-Vis), Fourier transformation infrared spectroscopy (FTIR), Particle size distribution (PSA), Scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy (EDS), Transmission electron microscopy (TEM) for structural optical and morphological studies respectively...
July 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28539409/spatial-analysis-of-cdc42-activity-reveals-a-role-for-plasma-membrane-associated-cdc42-in-centrosome-regulation
#9
Kari A Herrington, Andrew L Trinh, Carolyn Dang, Ellen O'Shaughnessy, Klaus M Hahn, Enrico Gratton, Michelle A Digman, Christine Sütterlin
The ability of the small GTPase Cdc42 to regulate diverse cellular processes depends on tight spatial control of its activity. Cdc42 function is best understood at the plasma membrane (PM), where it regulates cytoskeletal organization and cell polarization. Active Cdc42 has also been detected at the Golgi, but its role and regulation at this organelle is only partially understood. Here we analyze the spatial distribution of Cdc42 activity by monitoring the dynamics of the Cdc42 FLARE biosensor using the phasor approach to FLIM-FRET...
May 24, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28537256/design-of-synthetic-epigenetic-circuits-featuring-memory-effects-and-reversible-switching-based-on-dna-methylation
#10
Johannes A H Maier, Raphael Möhrle, Albert Jeltsch
Epigenetic systems store information in DNA methylation patterns in a durable but reversible manner, but have not been regularly used in synthetic biology. Here, we designed synthetic epigenetic memory systems using DNA methylation sensitive engineered zinc finger proteins to repress a memory operon comprising the CcrM methyltransferase and a reporter. Triggering by heat, nutrients, ultraviolet irradiation or DNA damaging compounds induces CcrM expression and DNA methylation. In the induced on-state, methylation in the operator of the memory operon prevents zinc finger protein binding leading to positive feedback and permanent activation...
May 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28536710/a-label-free-ratiometric-electrochemical-dna-sensor-for-monitoring-intracellular-redox-homeostasis
#11
Xinping Liu, Zhengqing Yan, Yuhuan Sun, Jinsong Ren, Xiaogang Qu
A label-free ratiometric electrochemical biosensor was constructed for monitoring intracellular redox homeostasis by DNA metallization-mediated hybridization chain reaction amplification. This platform could also be used to discriminate cancer cells and normal cells.
May 24, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28535471/graphene-oxide-supported-carbon-nanofiber-like-network-derived-from-polyaniline-a-novel-composite-for-enhanced-glucose-oxidase-bioelectrode-performance
#12
Zepeng Kang, Kailong Jiao, Xinping Xu, Ruiyun Peng, Shuqiang Jiao, Zongqian Hu
A three-dimensional architecture of PANI@GO hybrid was synthesized via in-situ polymerization of aniline monomers on the surface of graphene oxide (GO) and carbonized at 1600°C. The SEM images showed that surfaces of planar GO were covered by a compact nanofiber-like polyaniline (PANI) layer which presented an interconnected network. Nanofiber-like PANI on the GO surface was mostly preserved and became the carbon nanofibers (CNFs) after carbonization. The TEM images showed that the carbonized PANI grew seamlessly on the GO surface and served as conductive "network" between interlayers of GO...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28535341/fully-stretchable-optoelectronic-sensors-based-on-colloidal-quantum-dots-for-sensing-photoplethysmographic-signals
#13
Tae-Ho Kim, Chang-Seok Lee, Sangwon Kim, Jaehyun Hur, Sangmin Lee, Keun Wook Shin, Young-Zoon Yoon, Moon Kee Choi, Jiwoong Yang, Dae-Hyeong Kim, Taeghwan Hyeon, Seongjun Park, Sungwoo Hwang
Flexible and stretchable optoelectronic devices can be potentially applied in displays, biosensors, biomedicine, robotics, and energy generation. The use of nanomaterials with superior optical properties such as quantum dots (QDs) is important in the realization of wearable displays and biomedical devices, but specific structural design as well as selection of materials should preferentially accompany this technology to realize stretchable forms of these devices. Here, we report stretchable optoelectronic sensors manufactured using colloidal QDs and integrated with elastomeric substrates, whose optoelectronic properties are stable under various deformations...
May 23, 2017: ACS Nano
https://www.readbyqxmd.com/read/28534562/k-junction-structure-mediated-exponential-signal-amplification-strategy-for-microrna-detection-in-electrochemiluminescence-biosensor
#14
Xue Li, Anyi Chen, Yaqin Chai, Ruo Yuan
Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at the 5'-terminus. Thus, the activity of the reporter DNA could be inhibited and the recognition domain for the target microRNA constructed through the K-junction structure formed...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28534415/sequence-and-structure-dual-dependent-interaction-between-small-molecules-and-dna-for-the-detection-of-residual-silver-ions-in-as-prepared-silver-nanomaterials
#15
Zhaoyin Wang, Jian Zhao, Zijun Li, Jianchun Bao, Zhihui Dai
Investigations on interaction between small molecules and DNA are the basis of designing advanced bioanalytical systems. We herein propose a novel interaction between heterocyclic aromatic compounds (HACs) and single-stranded DNA (ssDNA). Taking methylene blue (MB) as a typical HAC, it is found that MB can interact with cytosine (C)-rich ssDNA in an enthalpy-driven process. The interaction between MB and C-rich ssDNA is sequence and structure dual-dependent: at least three consecutive C and single-stranded structure are necessary...
May 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28532684/quantitative-determination-of-testosterone-levels-with-biolayer-interferometry
#16
Hao Zhang, Wei Li, Hong Luo, Guangming Xiong, Yuanhua Yu
Natural and synthetic steroid hormones are widely spread in the environment and are considered as pollutants due to their endocrine activities, even at low concentrations, which are harmful to human health. To detect steroid hormones in the environment, a novel biosensor system was developed based on the principle of biolayer interferometry. Detection is based on changes in the interference pattern of white light reflected from the surface of an optical fiber with bound biomolecules. Monitoring interactions between molecules does not require radioactive, enzymatic, or fluorescent labels...
May 19, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28532147/development-of-a-bacterial-biosensor-for-rapid-screening-of-yeast-p-coumaric-acid-production
#17
Solvej Siedler, Narendar K Khatri, Andrea Zsohár, Inge Kjærbølling, Michael Vogt, Petter Hammar, Christian Førgaard Nielsen, Jan Marienhagen, Morten Otto Alexander O A Sommer, Haakan N Joensson
Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site...
May 23, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28532103/sensitive-impedimetric-dna-biosensor-based-on-nb-v-codoped-tio2-for-breast-cancer-susceptible-gene-detection
#18
Yu Wang, Xianwei Huang, He Li, Linlang Guo
In this paper, a novel (Nb,V) codoped TiO2 (NVTO) nanoparticle was synthesized, and a novel electrochemical DNA biosensor based on NVTO was first developed for detection of breast cancer susceptible gene detection. The proposed DNA biosensors were fabricated by immobilization of the probe DNA on a nanoporous CHIT-NVTO thin film deposited on an ITO-coated glass plate. The prepared NVTO composite film was characterized by XRD, SEM and FTIR. The results indicated that the synergistic effect of CHIT-NVTO nanocomposite can increase the electroactive surface area and the loading amounts of ssDNA...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531992/boronate-functionalized-hydrogel-as-a-novel-biosensing-interface-for-the-glycated-hemoglobin-a1c-hba1c-based-on-the-competitive-binding-with-signaling-glycoprotein
#19
Yong Duk Han, Ka Ram Kim, Yoo Min Park, Seung Yeon Song, Yong Ju Yang, Kangsun Lee, Yunhee Ku, Hyun C Yoon
According to recent increases in public healthcare costs associated with diabetes mellitus, the development of new glycemic monitoring techniques based on the biosensing of glycated hemoglobin A1c (HbA1c), a promising long-term glycemic biomarker, has become a major challenge. In the development of HbA1c biosensors for point-of-care applications, the selection of an effective biorecognition layer that provides a high reaction yield and specificity toward HbA1c is regarded as the most significant issue. To address this, we developed a novel HbA1c biosensing interfacial material by the integration of boronate hydrogel with glass fiber membrane...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531970/ultrasensitive-cholesterol-biosensor-based-on-enzymatic-silver-deposition-on-gold-nanoparticles-modified-screen-printed-carbon-electrode
#20
Yong Huang, Lijie Cui, Yewei Xue, Songbai Zhang, Nixuan Zhu, Jintao Liang, Guiyin Li
Cholesterol is one of the essential structural constituents of cell membranes. Determination of cholesterol is of great importance in clinical analysis because the level of cholesterol in serum is an indicator in the diagnosis and prevention of heart diseases. In this work, a simple and ultrasensitive cholesterol biosensor based on enzymatic silver deposition was designed by immobilizing cholesterol oxidase (CHOD) and cholesterol esterase (CHER) onto the surface of gold nanoparticles (Au NPs) modified screen-printed carbon electrode (SPE)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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