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https://www.readbyqxmd.com/read/29458204/a-review-of-implantable-biosensors-for-closed-loop-glucose-control-and-other-drug-delivery-applications
#1
Kee Scholten, Ellis Meng
Closed-loop drug delivery promises autonomous control of pharmacotherapy through the continuous monitoring of biomarker levels. For decades, researchers have strived for portable closed-loop systems, capable of treating ambulatory patients with chronic conditions such as diabetes mellitus. After years of development, the first of these systems have left the laboratory and entered commercial use. This long-awaited advance reflects recent development of chronically stable implantable biosensors, able to accurately measure biomarker levels in vivo...
February 16, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29458177/colorimetric-detection-of-microrna-based-on-dnazyme-and-nuclease-assisted-catalytic-hairpin-assembly-signal-amplification
#2
Hongmei Zhang, Kuiyu Wang, Shengjun Bu, Zhongyi Li, Chuanjing Ju, Jiayu Wan
Accurate and quantitative analysis of microRNA (miRNA) expression is critical for the diagnostics and theranostics of a disease. Herein, a proof-of-concept of a colorimetric horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) biosensor for miRNA assay based on nuclease-assisted catalytic hairpin assembly (CHA) signal amplification was demonstrated. Duplex-specific nuclease (DSN) was employed to cleave the single-stranded DNA (ssDNA) chimeric probe (CP) on the magnetic bead (MB) surface via hybridization of the CP and target miRNA...
February 16, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29458090/designing-fluorescent-biosensors-using-circular-permutations-of-riboswitches
#3
Johnny Truong, Yu-Fang Hsieh, Lynda Truong, Guifang Jia, Ming C Hammond
RNA-based fluorescent (RBF) biosensors have been applied to detect a variety of metabolites in vitro and in live cells. They are designed by combining the ligand sensing domain of natural riboswitches with in vitro selected fluorogenic aptamers. Different biosensor topologies have been developed to accommodate the diversity of riboswitch structures. Here we show that circular permutation of the riboswitch ligand sensing domain also gives functional biosensors, using the SAM-I riboswitch as our model. We reveal that this design can enhance fluorescence turn-on and ligand binding affinity compared to the non-permuted topology...
February 16, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29458004/real-time-analysis-of-cleavage-and-religation-activity-of-human-topoisomerase-1-based-on-ternary-fluorescence-resonance-energy-transfer-dna-substrate
#4
Zhenxing Wang, Hui Ouyang, Cinzia Tesauro, Alessio Ottaviani, Yong He, Paola Fiorani, Hui Xie, Alessandro Desideri, Zhifeng Fu
Human topoisomerase 1B is a ubiquitous and essential enzyme involved in relaxing the topological state of supercoiled DNA to allow the progression of fundamental DNA metabolism. Its enzymatic catalytic cycle consists of cleavage and religation reaction. A ternary fluorescence resonance energy transfer biosensor based on a suicide DNA substrate conjugated with three fluorophores has been developed to monitor both cleavage and religation Topoisomerase I catalytic function. The presence of fluorophores does not alter the specificity of the enzyme catalysis on the DNA substrate...
February 16, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29457531/shedding-new-light-on-rhoa-signalling-as-a-drug-target-in-vivo-using-a-novel-rhoa-fret-biosensor-mouse
#5
Max Nobis, David Herrmann, Sean C Warren, Douglas Strathdee, Thomas R Cox, Kurt I Anderson, Paul Timpson
The small GTPase RhoA is a master regulator of signalling in cell-extracellular matrix interactions. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis. Investigating RhoA activity in a native tissue environment is challenging using conventional biochemical methods; we therefore developed a RhoA-FRET biosensor mouse, employing the adaptable nature of intravital imaging to a variety of settings. Mechnotransduction was explored in the context of osteocyte processes embedded in the calvaria responding in a directional manner to compression stress...
February 18, 2018: Small GTPases
https://www.readbyqxmd.com/read/29455283/utilizing-hyaluronic-acid-as-a-versatile-platform-for-fluorescence-resonance-energy-transfer-based-glucose-sensing
#6
Minghao Ge, Pengli Bai, Mingli Chen, Jingjing Tian, Jun Hu, Xu Zhi, Huancai Yin, Jian Yin
Here, we utilized the ultrasonic emulsification technique to generate hyaluronic acid microspheres incorporating a fluorescence-based glucose biosensor. We synthesized a novel lanthanide ion luminophore based on Eu3+ . Eu sulfosuccinimidyl dextran (Eu-dextran) and Alexa Fluor 647 sulfosuccinimidyl-ConA (Alexa Fluor 647-ConA) were encapsulated in hyaluronic acid hydrogel to generate microspheres. Glucose sensing was carried out using a fluorescence resonance energy transfer (FRET)-based assay principle. A proportional fluorescence intensity increase was found within a 0...
February 17, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29455077/raman-studies-of-the-interactions-of-fibrous-carbon-nanomaterials-with-albumin
#7
Aleksandra Wesełucha-Birczyńska, Krzysztof Morajka, Ewa Stodolak-Zych, Elżbieta Długoń, Maria Dużyja, Tomasz Lis, Maciej Gubernat, Magdalena Ziąbka, Marta Błażewicz
Adsorption or immobilization of proteins on synthetic surfaces is a key issue in the context of the biocompatibility of implant materials, especially those intended for the needs of cardiac surgery but also for the construction of biosensors or nanomaterials used as drug carriers. The subject of research was the analysis of Raman spectra of two types of fibrous carbon nanomaterials, of great potential for biomedical applications, incubated with human serum albumin (HSA). The first nanomaterial has been created on the layer of MWCNTs deposited by electrophoretic method (EPD) and then covered by thin film of pyrolytic carbon introduced by chemical vapor deposition process (CVD)...
February 7, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29455028/novel-electrochemical-biosensor-based-on-cationic-peptide-modified-hemin-g-quadruples-enhanced-peroxidase-like-activity
#8
Qian Yu, Yongmei Wu, Zi Liu, Sheng Lei, Gaiping Li, Baoxian Ye
This work designed an artificial substrate peptide to synthesize peptide-hemin/G-quadruplex (peptide-DNAzyme) conjugates. In addition to enhancing catalytic activity of hemin/G-quadruplex, the peptide could also be induced and cleaved by prostate specific antigen (PSA). It was the first report on peptide-DNAzyme conjugates in application of the peptide biosensor. The polyethyleneimine-reduced graphene oxide@hollow platinum nanotubes (PEI-rGO@PtNTs) nanocomposites were cast on the glassy carbon electrode in order to form the interface of biocompatibility and huge surface area for bioprobes immobilization...
February 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29455024/all-carbon-suspended-nanowire-sensors-as-a-rapid-highly-sensitive-label-free-chemiresistive-biosensing-platform
#9
Aung Thiha, Fatimah Ibrahim, Shalini Muniandy, Ignatius Julian Dinshaw, Swe Jyan Teh, Kwai Lin Thong, Bey Fen Leo, Marc Madou
Nanowire sensors offer great potential as highly sensitive electrochemical and electronic biosensors because of their small size, high aspect ratios, and electronic properties. Nevertheless, the available methods to fabricate carbon nanowires in a controlled manner remain limited to expensive techniques. This paper presents a simple fabrication technique for sub-100 nm suspended carbon nanowire sensors by integrating electrospinning and photolithography techniques. Carbon Microelectromechanical Systems (C-MEMS) fabrication techniques allow fabrication of high aspect ratio carbon structures by patterning photoresist polymers into desired shapes and subsequent carbonization of resultant structures by pyrolysis...
February 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29454879/application-of-genetically-encoded-redox-biosensors-to-measure-dynamic-changes-in-the-glutathione-bacillithiol-and-mycothiol-redox-potentials-in-pathogenic-bacteria
#10
REVIEW
Quach Ngoc Tung, Nico Linzner, Vu Van Loi, Haike Antelmann
Gram-negative bacteria utilize glutathione (GSH) as their major LMW thiol. However, most Gram-positive bacteria do not encode enzymes for GSH biosynthesis and produce instead alternative LMW thiols, such as bacillithiol (BSH) and mycothiol (MSH). BSH is utilized by Firmicutes and MSH is the major LMW thiol of Actinomycetes. LMW thiols are required to maintain the reduced state of the cytoplasm, but are also involved in virulence mechanisms in human pathogens, such as Staphylococcus aureus, Mycobacterium tuberculosis, Streptococcus pneumoniae, Salmonella enterica subsp...
February 15, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29454758/optical-fiber-tips-for-biological-applications-from-light-confinement-biosensing-to-bioparticles-manipulation
#11
REVIEW
Joana S Paiva, Pedro A S Jorge, Carla C Rosa, João P S Cunha
BACKGROUND: The tip of an optical fiber has been considered an attractive platform in Biology. The simple cleaved end of an optical fiber can be machined, patterned and/or functionalized, acquiring unique properties enabling the exploitation of novel optical phenomena. Prompted by the constant need to measure and manipulate nanoparticles, the invention of the Scanning Near-Field Optical Microscopy (SNOM) triggered the optimization and development of novel fiber tip microfabrication methods...
February 15, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29453807/tunable-poly-methacrylic-acid-co-acrylamide-nanoparticles-through-inverse-emulsion-polymerization
#12
Justin X Zhong, John R Clegg, Eric W Ander, Nicholas A Peppas
Environmentally responsive biomaterials have played key roles in the design of biosensors and drug delivery vehicles. Their physical response to external stimuli, such as temperature or pH, can transduce a signal or trigger the release of a drug. In this work, we designed a robust, highly tunable, pH responsive nanoscale hydrogel system. We present the design and characterization of poly((methacrylic acid) - co - acrylamide) hydrogel nanoparticles, crosslinked with methylenebisacrylamide, through inverse emulsion polymerization...
February 17, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29452205/impaired-cerebral-microcirculation-induced-by-ammonium-chloride-in-rats-is-due-to-cortical-adenosine-release
#13
Peter Nissen Bjerring, Esben Jannik Bjerrum, Fin Stolze Larsen
BACKGROUND: Liver failure results in hyperammonaemia, impaired regulation of cerebral microcirculation, encephalopathy and death. However, the key mediator that alters cerebral microcirculation remains unidentified. In this study we show that topical ammonium significantly increases periarteriolar adenosine tone on the brain surface of healthy rats and is associated with a disturbed microcirculation. METHODS: Cranial windows were prepared in anaesthetized Wistar rats...
February 13, 2018: Journal of Hepatology
https://www.readbyqxmd.com/read/29451128/signal-amplifying-nanoparticle-hydrogel-hybrid-microarray-biosensor-for-metal-enhanced-fluorescence-detection-of-organophosphorus-compounds
#14
Minsu Kim, Kangwon Lee, Ji Eon Kwon, Won Gun Koh
In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds (OPs). The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica)...
February 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29450964/imparting-designer-biorecognition-functionality-to-metal-organic-frameworks-by-a-dna-mediated-surface-engineering-strategy
#15
Weiyu Ning, Zhenghan Di, Yingjie Yu, Pingmei Zeng, Chunzhi Di, Daquan Chen, Xueqian Kong, Guangjun Nie, Yuliang Zhao, Lele Li
Surface functionality is an essential component for processing and application of metal-organic frameworks (MOFs). A simple and cost-effective strategy for DNA-mediated surface engineering of zirconium-based nanoscale MOFs (NMOFs) is presented, capable of endowing them with specific molecular recognition properties and thus expanding their potential for applications in nanotechnology and biotechnology. It is shown that efficient immobilization of functional DNA on NMOFs can be achieved via surface coordination chemistry...
February 16, 2018: Small
https://www.readbyqxmd.com/read/29449298/clinical-validation-of-a-public-health-policy-making-platform-for-hearing-loss-evotion-protocol-for-a-big-data-study
#16
Giorgos Dritsakis, Dimitris Kikidis, Nina Koloutsou, Louisa Murdin, Athanasios Bibas, Katherine Ploumidou, Ariane Laplante-Lévesque, Niels Henrik Pontoppidan, Doris-Eva Bamiou
INTRODUCTION: The holistic management of hearing loss (HL) requires an understanding of factors that predict hearing aid (HA) use and benefit beyond the acoustics of listening environments. Although several predictors have been identified, no study has explored the role of audiological, cognitive, behavioural and physiological data nor has any study collected real-time HA data. This study will collect 'big data', including retrospective HA logging data, prospective clinical data and real-time data via smart HAs, a mobile application and biosensors...
February 15, 2018: BMJ Open
https://www.readbyqxmd.com/read/29448635/surface-plasmon-resonance-aptamer-biosensor-for-discriminating-pathogenic-bacteria-vibrio-parahaemolyticus
#17
Ji-Young Ahn, Kyeong-Ah Lee, Moon-Jong Lee, Simranjeet Singh Sekhon, Sung-Keun Rhee, Sung-Jin Cho, Jung Ho Ko, Lyon Lee, Janet Han, Sang Yong Kim, Jiho Min, Yang-Hoon Kim
In this paper, whole-bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Vibrio parahaemolyticus. Round selection for V. parahaemolyticus was conducted 11 rounds, including two negative selection rounds. It was determined through real-time PCR amplification and post-SELEX experiment. The selected aptmers had high binding property and specificity to V. parahaemolyticus. Of 28 aptamers tested, VPCA-apta#1 had the highest binding affinity compared to other aptamer candidates obtained...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448537/immunoreaction-mediated-aggregation-of-gold-nanoparticles-for-sensitive-and-selective-assay-of-hepatitis-b-surface-antigen
#18
Dinh-Vu Le, Van-Tan Le, Van-Cuong Nguyen, Jian-Hui Jiang, Kim-Triet Dang
A rapid, sensitive and quantitative assay method for Hepatitis B surface antigen (HBsAg) is of paramount importance for the drug development and in the diagnosis of this disease. Here, we proposed a novel biosensor that sensitively and selectively screen Hepatitis B surface antigen. This strategy relies on the cross-linking aggregation of gold nanoparticles (AuNPs) that were decorated with Hepatitis B surface antibody (HBsAb) and Raman reporter 5-thio-nitrobenzoic acid (TNB) by Hepatitis B surface antigen. The immune reaction between HBsAb and HBsAg offers this strategy high specificity, and the use of AuNPs additionally allows a visual and homogeneous assay format, thus permitting improved simplicity and throughput of the assays...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448225/a-dye-sensitized-solar-cell-acting-as-the-electrical-reading-box-of-an-immunosensor-application-to-cea-determination
#19
Liliana A A N A Truta, Felismina T C Moreira, M Goreti F Sales
Monitoring cancer biomarkers in biological fluids has become a key tool for disease diagnosis, which should be of easy access anywhere in the world. The possibility of reducing basic requirements in the field of electrochemical biosensing may open doors in this direction. This work proposes for this purpose an innovative electrochemical immunosensing system using a photovoltaic cell as an electrical reading box. Immunosensing ensures accuracy, the electrochemical-ground of the device ensures sensitivity and detectability, and the photovoltaic cell drives the system towards electrical autonomy...
February 7, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29448223/2d-transition-metal-carbide-mxene-as-a-robust-biosensing-platform-for-enzyme-immobilization-and-ultrasensitive-detection-of-phenol
#20
Lingxia Wu, Xianbo Lu, Dhanjai, Zhong-Shuai Wu, Yanfeng Dong, Xiaohui Wang, Shuanghao Zheng, Jiping Chen
MXene-Ti 3 C 2 , as a new class of two-dimensional (2D) transition metal carbides (or nitrides), has been synthesized by exfoliating pristine Ti 3 AlC 2 phases with hydrofluoric acid. The SEM and XRD images show that the resultant MXene possesses a graphene-like 2D nanostructure. and the surface of MXene has been partially terminated with -OH, thus providing a favorable microenvironment for enzyme immobilization and retaining their bioactivity and stability. Considering the unique metallic conductivity, biocompatibility and good dispersion in aqueous phase, the as-prepared MXene was explored as a new matrix to immobilize tyrosinase (a model enzyme) for fabricating a mediator-free biosensor for ultrasensitive and rapid detection of phenol...
February 8, 2018: Biosensors & Bioelectronics
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