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https://www.readbyqxmd.com/read/28101430/projection-method-for-improving-signal-to-noise-ratio-of-localized-surface-plasmon-resonance-biosensors
#1
Ahmed Abumazwed, Wakana Kubo, Chen Shen, Takuo Tanaka, Andrew G Kirk
This paper presents a simple and accurate method (the projection method) to improve the signal to noise ratio of localized surface plasmon resonance (LSPR). The nanostructures presented in the paper can be readily fabricated by nanoimprint lithography. The finite difference time domain method is used to simulate the structures and generate a reference matrix for the method. The results are validated against experimental data and the proposed method is compared against several other recently published signal processing techniques...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28101426/asymmetric-split-h-shape-nanoantennas-for-molecular-sensing
#2
I G Mbomson, S Tabor, B Lahiri, G Sharp, S G McMeekin, R M De La Rue, N P Johnson
In this paper we report on a very sensitive biosensor based on gold asymmetric nanoantennas that are capable of enhancing the molecular resonances of C-H bonds. The nanoantennas are arranged as arrays of asymmetric-split H-shape (ASH) structures, tuned to produce plasmonic resonances with reflectance double peaks within the mid-infrared vibrational resonances of C-H bonds for the assay of deposited films of the molecule 17β-estradiol (E2), used as an analyte. Measurements and numerical simulations of the reflectance spectra have enabled an estimated enhancement factor on the order of 10(5) to be obtained for a thin film of E2 on the ASH array...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28101401/human-heart-failure-biomarker-immunosensor-based-on-excessively-tilted-fiber-gratings
#3
Binbin Luo, Shengxi Wu, Zhonghao Zhang, Wengen Zou, Shenghui Shi, Mingfu Zhao, Nianbing Zhong, Yong Liu, Xue Zou, Lingling Wang, Weina Chai, Chuanmin Hu, Lin Zhang
A label-free immunosensor platform based on excessively tilted fiber gratings (Ex-TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NT-proBNP...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28098973/electrochemiluminescence-detection-of-escherichia-coli-o157-h7-based-on-a-novel-polydopamine-surface-imprinted-polymer-biosensor
#4
Shufan Chen, Xueqian Chen, Lijuan Zhang, Juanjuan Gao, Qiang Ma
In this paper, a facilely prepared electrochemiluminescence (ECL) biosensor was developed for Escherichia coli (E. coli) O157:H7 quantitative detection based on polydopamine (PDA) surface imprinted polymer (SIP) and nitrogen-doped graphene quantum dots (N-GQDs). N-GQDs with a high quantum yield of 43.2% were synthesized. The uniform PDA SIP film for E. coli O157:H7 was established successfully with a facile route. The dopamine and E. coli O157:H7 were mixed and electropolymerized directly on the electrode. After removed E...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28098753/reagent-less-and-robust-biosensor-for-direct-determination-of-lactate-in-food-samples
#5
Iria Bravo, Mónica Revenga-Parra, Félix Pariente, Encarnación Lorenzo
Lactic acid is a relevant analyte in the food industry, since it affects the flavor, freshness, and storage quality of several products, such as milk and dairy products, juices, or wines. It is the product of lactose or malo-lactic fermentation. In this work, we developed a lactate biosensor based on the immobilization of lactate oxidase (LOx) onto N,N'-Bis(3,4-dihydroxybenzylidene) -1,2-diaminobenzene Schiff base tetradentate ligand-modified gold nanoparticles (3,4DHS-AuNPs) deposited onto screen-printed carbon electrodes, which exhibit a potent electrocatalytic effect towards hydrogen peroxide oxidation/reduction...
January 13, 2017: Sensors
https://www.readbyqxmd.com/read/28097744/in-situ-electrical-monitoring-of-cancer-cells-invading-vascular-endothelial-cells-with-semiconductor-based-biosensor
#6
Toshiya Sakata, Yusuke Matsuse
Cellular dynamics is very closely related to ionic behaviors, most of which have been hardly monitored in real time, whereas semiconductor-based biosensors have the unique advantage of direct detection of ionic charges in a real-time and noninvasive manner. In this study, we monitored the invasion process of cancer cells into the vascular endothelial layer in real time by a label-free method using a field-effect transistor (FET) biosensor. Endothelial cells were cultured on the sensing surface of the FET gate, to form a basement membrane between the endothelial cells and the sensing surface...
January 18, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28097171/structural-insights-into-the-effects-of-charge-reversal-substitutions-at-the-surface-of-horseradish-peroxidase
#7
Leila Navapour, Navid Mogharrab
Horseradish peroxidase (HRP), has gained significant interests in biotechnology, especially in biosensor field and diagnostic test kits. Hence, its solvent-exposed lysine residues 174, 232, and 241 have been frequently modified with the aim of improving its stability and catalytic efficiency. In this computational study, we investigated the effects of Lys-to-Glu substitutions on HRP structure to model charge-reversal manipulations at the enzyme surface. Simulation results implied that upon these substitutions, the number of stable hydrogen bonds and α-helical content of HRP are increased and the proximal Ca2+ binding pocket becomes more integrated...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28096602/surface-plasmon-resonance-based-biosensor-a-new-platform-for-rapid-diagnosis-of-livestock-diseases
#8
REVIEW
Pravas Ranjan Sahoo, Parthasarathi Swain, Sudhanshu Mohan Nayak, Sudam Bag, Smruti Ranjan Mishra
Surface plasmon resonance (SPR) based biosensors are the most advanced and developed optical label-free biosensor technique used for powerful detection with vast applications in environmental protection, biotechnology, medical diagnostics, drug screening, food safety, and security as well in livestock sector. The livestock sector which contributes the largest economy of India, harbors many bacterial, viral, and fungal diseases impacting a great loss to the production and productive potential which is a major concern in both small and large ruminants...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096507/de-novo-phosphorylation-and-conformational-opening-of-the-tyrosine-kinase-lck-act-in-concert-to-initiate-t-cell-receptor-signaling
#9
Lars Philipsen, Amarendra V Reddycherla, Roland Hartig, Janine Gumz, Matthias Kästle, Andreas Kritikos, Mateusz P Poltorak, Yury Prokazov, Evgeny Turbin, André Weber, Werner Zuschratter, Burkhart Schraven, Luca Simeoni, Andreas J Müller
The enzymatic activity of the Src family tyrosine kinase p56(Lck) (Lck) is tightly controlled by differential phosphorylation of two tyrosine residues, Tyr(394) and Tyr(505) Phosphorylation of Tyr(394) and the conformational opening of Lck are believed to activate the kinase, whereas Tyr(505) phosphorylation is thought to generate a closed, inactive conformation of Lck. We investigated whether the conformation of Lck and its phosphorylation state act in concert to regulate the initiation of T cell receptor (TCR) signaling...
January 17, 2017: Science Signaling
https://www.readbyqxmd.com/read/28096132/innovative-techniques-for-evaluating-behavioral-nutrition-interventions
#10
REVIEW
Rachel E Scherr, Kevin D Laugero, Dan J Graham, Brian T Cunningham, Lisa Jahns, Karina R Lora, Marla Reicks, Amy R Mobley
Assessing outcomes and the impact from behavioral nutrition interventions has remained challenging because of the lack of methods available beyond traditional nutrition assessment tools and techniques. With the current high global obesity and related chronic disease rates, novel methods to evaluate the impact of behavioral nutrition-based interventions are much needed. The objective of this narrative review is to describe and review the current status of knowledge as it relates to 4 different innovative methods or tools to assess behavioral nutrition interventions...
January 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/28095570/efficacy-of-a-skin-condition-adapted-solution-for-xerosis-and-itch-relief-associated-with-aging
#11
Ramsin Joseph Yadgar, Adam J Friedman
In recent decades, the stratum corneum (SC), has been recognized for its multifunctional role in maintaining the homeostasis of the human epidermal barrier. A better understanding of the SC's ability to act as its own biosensor in detecting dysfunction and integrating restorative actions can help identify the origin of certain skin conditions. A more holistic understanding of the morphological changes of the SC during the natural aging process and how it deviates in disease states can help bring about new treatment strategies...
November 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28095257/label-free-affinity-biosensor-arrays-novel-technology-for-molecular-diagnostics
#12
Steven Johnson, Thomas F Krauss
No abstract text is available yet for this article.
January 17, 2017: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/28094510/nanoporous-gold-based-electrode-morphology-libraries-for-investigating-structure-property-relationships-in-nucleic-acid-based-electrochemical-biosensors
#13
Zimple Matharu, Pallavi Daggumati, Ling Wang, Tatiana S Dorofeeva, Zidong Li, Erkin Seker
Nanoporous gold (np-Au) electrode coatings significantly enhance performance of electrochemical nucleic acid biosensors due to their three-dimensional nanoscale network, high electrical conductivity, facile surface functionalization, and biocompatibility. Contrary to planar electrodes, the np-Au electrodes also exhibit sensitive detection in the presence of common biofouling media due to their porous structure. However, the pore size of the nano-matrix plays a critical role in dictating the extent of biomolecular capture and transport...
January 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28094391/advantages-of-an-optical-nanosensor-system-for-the-mechanistic-analysis-of-a-novel-topoisomerase-i-targeting-drug-a-case-study
#14
Marie B Andersen, Cinzia Tesauro, María Gonzalez, Emil L Kristoffersen, Concepción Alonso, Gloria Rubiales, Andrea Coletta, Rikke Frøhlich, Magnus Stougaard, Yi-Ping Ho, Francisco Palacios, Birgitta R Knudsen
The continuous need for the development of new small molecule anti-cancer drugs calls for easily accessible sensor systems for measuring the effect of vast numbers of new drugs on their potential cellular targets. Here we demonstrate the use of an optical DNA biosensor to unravel the inhibitory mechanism of a member of a new family of small molecule human topoisomerase I inhibitors, the so-called indeno-1,5-naphthyridines. By analysing human topoisomerase I catalysis on the biosensor in the absence or presence of added drug complemented with a few traditional assays, we demonstrate that the investigated member of the indeno-1,5-naphthyridine family inhibited human topoisomerase I activity by blocking enzyme-DNA dissociation...
January 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28094255/specific-and-sensitive-isothermal-electrochemical-biosensor-for-plant-pathogen-dna-detection-with-colloidal-gold-nanoparticles-as-probes
#15
Han Yih Lau, Haoqi Wu, Eugene J H Wee, Matt Trau, Yuling Wang, Jose R Botella
Developing quick and sensitive molecular diagnostics for plant pathogen detection is challenging. Herein, a nanoparticle based electrochemical biosensor was developed for rapid and sensitive detection of plant pathogen DNA on disposable screen-printed carbon electrodes. This 60 min assay relied on the rapid isothermal amplification of target pathogen DNA sequences by recombinase polymerase amplification (RPA) followed by gold nanoparticle-based electrochemical assessment with differential pulse voltammetry (DPV)...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28093984/polymer-conjugated-gold-nanoparticles-in-biomedical-applications
#16
Stanley Anniebell, Subash C B Gopinath
Research interests on the properties of polymer conjugated gold nanoparticle (GNP) in biomedicine are rapidly raised because of the extensive proof of their unique properties. In the field of biomedicine, GNPs are widely used because of their inertness and low levels of cytotoxicity. Therefore, when exposed to cells, they are less prone to exert damaging effects. GNPs are capable of being functionalized as desired and are ideal as it does not encourage undesired side reactions that might counter react with the initial intention of the functionalization...
January 16, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28093826/highly-sensitive-detection-of-e2-activity-in-ubiquitination-using-an-artificial-ring-finger
#17
Kazuhide Miyamoto, Miho Sumida, Mayumi Yuasa-Sunagawa, Kazuki Saito
The ubiquitin-conjugating (E2) enzymes of protein ubiquitination are associated with various diseases such as leukemia, lung cancer, and breast cancer. Rapid and accurate detection of E2 enzymatic activities remains poor. Here, we described the detection of E2 activity on a signal accumulation ISFET biosensor (AMIS sensor) using an artificial RING finger (ARF). The use of ARF enables the simplified detection of E2 activity without a substrate. The high-sensitivity quantitative detection of E2 activities was demonstrated via real-time monitoring over a response range of femtomolar to micromolar concentrations...
January 17, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28093127/magnetic-beads-based-dna-hybridization-chain-reaction-amplification-and-dnazyme-recognition-for-colorimetric-detection-of-uranyl-ion-in-seafood
#18
Hongyan Zhang, Xian Cheng, Lian Chen, Fan Mo, LiangJun Xu, FengFu Fu
A novel colorimetric biosensor, which employs DNAzyme-functionalized magnetic beads (MBs) as recognition probe, enzyme-assisted catalytic oxidation of TMB (3,3',5,5'-tetramethylbenzidine sulfate) as signal and DNA hybridization chain reaction as amplification strategy, has been developed for detecting trace uranyl ion (UO2(2+)) in seafood and aqueous environment with high sensitivity and specificity. We demonstrated that UO2(2+) can specifically cleave DNAzyme immobilized on MBs surface to release a short single-strand DNA (primer), and the released primer trigger DNA hybridization chain reaction to form a long one dimensional DNA concatamer on the MBs surface...
March 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28092361/designed-cell-consortia-as-fragrance-programmable-analog-to-digital-converters
#19
Marius Müller, Simon Ausländer, Andrea Spinnler, David Ausländer, Julian Sikorski, Marc Folcher, Martin Fussenegger
Synthetic biology advances the rational engineering of mammalian cells to achieve cell-based therapy goals. Synthetic gene networks have nearly reached the complexity of digital electronic circuits and enable single cells to perform programmable arithmetic calculations or to provide dynamic remote control of transgenes through electromagnetic waves. We designed a synthetic multilayered gaseous-fragrance-programmable analog-to-digital converter (ADC) allowing for remote control of digital gene expression with 2-bit AND-, OR- and NOR-gate logic in synchronized cell consortia...
January 16, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28092358/recurrent-rna-motifs-as-scaffolds-for-genetically-encodable-small-molecule-biosensors
#20
Ely B Porter, Jacob T Polaski, Makenna M Morck, Robert T Batey
Allosteric RNA devices are increasingly being viewed as important tools capable of monitoring enzyme evolution, optimizing engineered metabolic pathways, facilitating gene discovery and regulators of nucleic acid-based therapeutics. A key bottleneck in the development of these platforms is the availability of small-molecule-binding RNA aptamers that robustly function in the cellular environment. Although aptamers can be raised against nearly any desired target through in vitro selection, many cannot easily be integrated into devices or do not reliably function in a cellular context...
January 16, 2017: Nature Chemical Biology
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