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https://www.readbyqxmd.com/read/27913198/argininosuccinate-synthetase-1-ass1-is-a-common-metabolic-marker-of-chemosensitivity-for-targeted-arginine-and-glutamine-starvation-therapy
#1
Yan Long, Wen-Bin Tsai, Dajuan Wang, David H Hawke, Niramol Savaraj, Lynn G Feun, Mien-Chie Hung, Helen H W Chen, Macus Tien Kuo
Argininosuccinate synthetase 1 (ASS1) is the rate-limiting enzyme that catalyzes the biosynthesis of arginine (Arg). Many malignant human tumors are auxotrophic for Arg because ASS1 is silenced. ASS1 has been established as a sensor of Arg auxotrophic response and a chemosensitivity marker for Arg starvation therapy. Here, we report that ASS1 is also a sensor for glutamine (Gln)-deprivation response, and that upregulation of ASS1 expression is associated with resistance to Gln-starvation treatments. Knockdown of ASS1 expression resulted in increased sensitivity to both Arg- and Gln-starvation, whereas increased ASS1 expression by ectopic transfection is associated with resistance to both Arg- and Gln-starvation...
November 29, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27911494/determining-functional-aptamer-protein-interaction-by-biolayer-interferometry
#2
Xinhui Lou, Martin Egli, Xianbin Yang
Short single-stranded nucleic acids called aptamers are widely being explored as recognition molecules of high affinity and specificity for binding a wide range of target molecules, particularly protein targets. In biolayer interferometry (BLI), a simple Dip-and-Read approach in which the aptamer-coated biosensors are dipped into microplate wells is used to study the interactions between an aptamer and its target protein. Here we describe the protocol for the analysis of the interaction between a well-characterized anti-thrombin RNA aptamer with thrombin (Basic Protocol)...
December 1, 2016: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/27911492/rolling-circle-amplification-with-chemically-modified-nucleoside-triphosphates
#3
Marcel Hollenstein, Masad J Damha
Modified nucleoside triphosphates (dN*TPs) represent facile and versatile precursors for the introduction of chemical diversity into nucleic acids. While dN*TPs have been utilized in a plethora of practical applications, very little attention has been devoted to the assessment of their compatibility with isothermal amplification strategies. In this context, rolling circle amplification (RCA) is a wide-spread enzymatic replication method in which small single-stranded DNA (ssDNA) circles serve as templates in primer extension reactions yielding very long, ssDNA products...
December 1, 2016: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/27911052/synthesis-and-cytotoxicity-of-dendritic-platinum-nanoparticles-with-hek-293-cells
#4
Kyubin Shim, Jeonghun Kim, Yoon-Uk Heo, Bo Jiang, Cuiling Li, Mohammed Shahabuddin, Kevin C-W Wu, Md Shahriar A Hossain, Yusuke Yamauchi, Jung Ho Kim
Dendritic platinum nanoparticles (DPNs) have been synthesized from l-ascorbic acid and an amphiphilic non-ionic surfactant (Brij-58) via a sonochemical method. The particle size and shape of the DPNs could be tuned by changing the reduction temperature, resulting in a uniform DPN with a size of 23 nm or 60 nm. The facets of DPNs have been studied by high-resolution transmission electron microscopy. The cytotoxicity of DPNs has been investigated using human embryonic kidney cells (HEK-293), and the biological adaptability exhibited by DPNs has opened a pathway to biomedical applications such as drug-delivery systems, photothermal treatment, and biosensors...
December 2, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27910742/review-article-fabricated-microparticles-an-innovative-method-to-minimize-the-side-effects-of-nsaids-in-arthritis
#5
Shaivad Shabee Hulhasan Abadi, Afrasim Moin, Gangadharappa Hosahalli Veerabhadrappa
Microparticles are polymeric bodies ranging 1-1000 µm that constitute a variety of forms such as microcapsules, microspheres, microcages, microshells, microrods, biosensors microparticles, radiolabeled microparticles, and so forth. This review focuses on general microparticles, mainly microcapsules and microspheres. Nonsteriodal anti-inflammatory drugs (NSAIDs) are one of the mostcommonly prescribed medications in the world. Most of the NSAIDs available have severe side effects. With increased awareness of NSAID-induced gastrointestinal (GI) side effects, safety has become a priority in treatment of arthritis and other inflammatory diseases with NSAIDs...
2016: Critical Reviews in Therapeutic Drug Carrier Systems
https://www.readbyqxmd.com/read/27909898/two-dimensional-polyaniline-nanostructure-to-the-development-of-microfluidic-integrated-flexible-biosensors-for-biomarker-detection
#6
Pei Liu, Yisi Zhu, Seung Hee Lee, Minhee Yun
In this work, we report a flexible field-effect-transistor (FET) biosensor design based on two-dimensional (2-D) polyaniline (PANI) nanostructure. The flexible biosensor devices were fabricated through a facile and inexpensive method that combines top-down and bottom-up processes. The chemically synthesized PANI nanostructure showed excellent p-type semiconductor properties as well as good compatibility with flexible design. With the 2-D PANI nanostructure being as thin as 80 nm and its extremely large surface-area-to-volume (SA/V) ratio due to the intrinsic properties of PANI chemical synthesis, the developed flexible biosensor exhibited outstanding sensing performance in detecting B-type natriuretic peptide (BNP) biomarkers, and was able to achieve high specificity (averagely 112 folds) with the limit of detection as low as 100 pg/mL...
December 2016: Biomedical Microdevices
https://www.readbyqxmd.com/read/27908723/oxidation-inhibits-pth-receptor-signaling-and-trafficking
#7
Juan A Ardura, Verónica Alonso, Pedro Esbrit, Peter A Friedman
Reactive Oxygen Species (ROS) increase during aging, potentially affecting many tissues including brain, heart, and bone. ROS alter signaling pathways and constitute potential therapeutic targets to limit oxidative damaging effects in aging-associated diseases. Parathyroid hormone receptors (PTHR) are widely expressed and PTH is the only anabolic therapy for osteoporosis. The effects of oxidative stress on PTHR signaling and trafficking have not been elucidated. Here, we used Fluorescence Resonance Energy Transfer (FRET)-based cAMP, ERK, and calcium fluorescent biosensors to analyze the effects of ROS on PTHR signaling and trafficking by live-cell imaging...
November 28, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27906289/mesoporous-nanospheres-functionalized-optical-microfiber-biosensor-for-low-concentration-neurotransmitter-detection
#8
Mingfei Ding, Yunyun Huang, Tuan Guo, Li-Peng Sun, Bai-Ou Guan
A label-free and ultrasensitive microfiber interferometer biosensor has been demonstrated for detection of neurotransmitter molecule (5-HT). The surface morphology of the silicon dioxide nanospheres acting as molecule sieve provides an effective mean of gathering 5-HT molecules by designed mesoporous structure. The slight concentration change of 5-HT molecules is translated into a dramatic wavelength shift of the interferometric fringe pattern. The experimental results show that the biosensor has a linear response in concentration range from 100 fM to 1 µM and a detection limit as low as 84 fM...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27906205/resonant-nanopillars-arrays-for-label-free-biosensing
#9
Ana L Hernández, Rafael Casquel, Miguel Holgado, Iñaki Cornago, Fátima Fernández, Paula Ciaurriz, Francisco J Sanza, Beatriz Santamaría, María V Maigler, María Fe Laguna
In our previous work we demonstrated for the first time, to the best of our knowledge, the experimental capability of resonant nanopillars (R-NP) arrays as biochemical transducers. In this Letter, we provide evidence of the capability and suitability of R-NP arrays on a chip to function as label-free optical multiplexed biosensors. R-NP are based on Si<sub>3</sub>N<sub>4</sub>/SiO<sub>2</sub> Bragg reflectors with a cavity of SiO<sub>2</sub>. In order to demonstrate the biosensing performance, R-NP were biofunctionalized by the immobilization of IgG antibodies acting as a bioreceptor...
December 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27905857/recent-development-in-sample-preparation-and-analytical-techniques-for-determination-of-quinolone-residues-in-food-products
#10
Zhichao Zhang, Hefa Cheng
Used heavily in food animal production, quinolones occur widely in food products of animal origin. Development of highly sensitive and selective analytical techniques for the detection of quinolone residues, often at trace levels, in food samples is necessary to ensure food safety and understand their public health risk. With complex matrices, food samples typically require a series of pretreatment steps, such as powdering, homogenization, deproteinization, and filtration. This review summarizes the recent advances in extraction, concentration, and detection techniques for quinolones in food product samples, and briefly compares the advantages and limitations of major techniques...
December 1, 2016: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/27905300/intravital-imaging-of-mouse-urothelium-reveals-activation-of-extracellular-signal-regulated-kinase-by-stretch-induced-intravesical-release-of-atp
#11
Takeshi Sano, Takashi Kobayashi, Hiromitsu Negoro, Atsushi Sengiku, Takuya Hiratsuka, Yuji Kamioka, Louis S Liou, Osamu Ogawa, Michiyuki Matsuda
To better understand the roles played by signaling molecules in the bladder, we established a protocol of intravital imaging of the bladder of mice expressing a Förster/fluorescence resonance energy transfer (FRET) biosensor for extracellular signal-regulated kinase (ERK), which plays critical roles not only in cell growth but also stress responses. With an upright two-photon excitation microscope and a vacuum-stabilized imaging window, cellular ERK activity was visualized in the whole bladder wall, from adventitia to urothelium...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27904938/peptide-based-fluorescence-biosensors-for-detection-measurement-of-nanoparticles
#12
Oluyemi Akinloye, Ramanarayan Krishnamurthy, David Wishart, Greg G Goss
The ability to detect and quantify nanoparticles is essential but there is currently no simple, sensitive, and rapid method for the detection of nanomaterials. We have developed a novel peptide-based fluorescence-based biosensor for detection and measurement of negatively charged engineered nanoparticles (ENPs). A peptide biosensor (seven lysine residues linked to a cysteine through a three glycine residue linker) with attached fluorescent probes-fluorescein-5-maleimide (F5M) and tetramethylrhodamine-5-maleimide (TMR5M)-was constructed...
November 30, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27904576/wearable-biosensors-for-monitoring-patients
#13
Sima Ajami, Fotooheh Teimouri
No abstract text is available yet for this article.
2016: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/27904390/ascorbate-oxidase-based-amperometric-biosensor-for-l-ascorbic-acid-determination-in-beverages
#14
Gábor Csiffáry, Péter Fűtő, Nóra Adányi, Attila Kiss
A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27904092/development-and-biosensor-applications-of-novel-functional-electrodes
#15
Shigehiro Takahashi
 Phenylboronic acid (PBA) derivatives have been used as alternatives to enzymes in the development of sugar-sensitive systems because the optical and electrochemical properties of these derivatives are significantly modulated upon sugar binding. This article reviews the voltammetric properties of sugar sensors prepared using dithiobis (4-butyrylamino-m-phenylboronic acid) (DTBA-PBA)-modified electrodes and PBA-appended layer-by-layer film-modified electrodes. In addition, the redox properties of reduced graphene oxide (rGO)-modified glassy carbon electrodes (GCEs) are discussed...
2016: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/27902940/a-novel-lab-on-chip-platform-with-integrated-solid-phase-pcr-and-supercritical-angle-fluorescence-saf-microlens-array-for-highly-sensitive-and-multiplexed-pathogen-detection
#16
Tran Quang Hung, Wai Hoe Chin, Yi Sun, Anders Wolff, Dang Duong Bang
Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detection technology. In this paper, we addressed this challenge by combining the SP-PCR with super critical angle fluorescence (SAF) microlens array embedded in a microchip. We fabricated miniaturized SAF microlens array as part of a microfluidic chamber in thermoplastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array...
November 12, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27901131/porous-silicon-based-biosensors-towards-real-time-optical-detection-of-target-bacteria-in-the-food-industry
#17
Naama Massad-Ivanir, Giorgi Shtenberg, Nitzan Raz, Christel Gazenbeek, Dries Budding, Martine P Bos, Ester Segal
Rapid detection of target bacteria is crucial to provide a safe food supply and to prevent foodborne diseases. Herein, we present an optical biosensor for identification and quantification of Escherichia coli (E. coli, used as a model indicator bacteria species) in complex food industry process water. The biosensor is based on a nanostructured, oxidized porous silicon (PSi) thin film which is functionalized with specific antibodies against E. coli. The biosensors were exposed to water samples collected directly from process lines of fresh-cut produce and their reflectivity spectra were collected in real time...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901090/fluorescence-tunable-ag-dna-biosensor-with-tailored-cytotoxicity-for-live-cell-applications
#18
Nelli Bossert, Donny de Bruin, Maria Götz, Dirk Bouwmeester, Doris Heinrich
DNA-stabilized silver clusters (Ag-DNA) show excellent promise as a multi-functional nanoagent for molecular investigations in living cells. The unique properties of these fluorescent nanomaterials allow for intracellular optical sensors with tunable cytotoxicity based on simple modifications of the DNA sequences. Three Ag-DNA nanoagent designs are investigated, exhibiting optical responses to the intracellular environments and sensing-capability of ions, functional inside living cells. Their sequence-dependent fluorescence responses inside living cells include (1) a strong splitting of the fluorescence peak for a DNA hairpin construct, (2) an excitation and emission shift of up to 120 nm for a single-stranded DNA construct, and (3) a sequence robust in fluorescence properties...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27899691/high-throughput-spectral-and-lifetime-based-fret-screening-in-living-cells-to-identify-small-molecule-effectors-of-serca
#19
Tory M Schaaf, Kurt C Peterson, Benjamin D Grant, Prachi Bawaskar, Samantha Yuen, Ji Li, Joseph M Muretta, Gregory D Gillispie, David D Thomas
A robust high-throughput screening (HTS) strategy has been developed to discover small-molecule effectors targeting the sarco/endoplasmic reticulum calcium ATPase (SERCA), based on a fluorescence microplate reader that records both the nanosecond decay waveform (lifetime mode) and the complete emission spectrum (spectral mode), with high precision and speed. This spectral unmixing plate reader (SUPR) was used to screen libraries of small molecules with a fluorescence resonance energy transfer (FRET) biosensor expressed in living cells...
November 29, 2016: Journal of Biomolecular Screening
https://www.readbyqxmd.com/read/27898020/multivalent-aptamers-versatile-tools-for-diagnostic-and-therapeutic-applications
#20
REVIEW
Mariya Vorobyeva, Pavel Vorobjev, Alya Venyaminova
Nucleic acid aptamers generated through an in vitro selection are currently extensively applied as very valuable biomolecular tools thanks to their prominent advantages. Diversity of spatial structures, ease of production through chemical synthesis and a large variety of chemical modifications make aptamers convenient building blocks for the generation of multifunctional constructs. An opportunity to combine different aptamer functionalities with other molecules of interest such as reporter groups, nanoparticles, chemotherapeutic agents, siRNA or antisense oligonucleotides provides a widest range of applications of multivalent aptamers...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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