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https://www.readbyqxmd.com/read/30024920/modeling-apc-mutagenesis-and-familial-adenomatous-polyposis-using-human-ips-cells
#1
Cesar A Sommer, Amalia Capilla, Francisco J Molina-Estevez, Andreia Gianotti-Sommer, Nicholas Skvir, Ignacio Caballero, Sanjib Chowdhury, Gustavo Mostoslavsky
Mutations in the gene Adenomatous Polyposis Coli or APC appear in most sporadic cases of colorectal cancer and it is the most frequent mutation causing hereditary Familial Adenomatous Polyposis. The detailed molecular mechanism by which APC mutations predispose to the development of colorectal cancer is not completely understood. This is in part due to the lack of accessibility to appropriate models that recapitulate the early events associated with APC mediated intestinal transformation. We have established a novel platform utilizing human induced Pluripotent Stem cells or iPSC from normal or FAP-specific APC mutant individuals and evaluated the effect of the mutation in the cells before and after differentiation into intestinal organoids...
2018: PloS One
https://www.readbyqxmd.com/read/30018468/the-role-of-micrornas-in-embryonic-and-induced-pluripotency
#2
REVIEW
Abbas Beh-Pajooh, Tobias Cantz
Research on stem cells is one of the fastest growing areas of regenerative medicine that paves the way for a comprehensive solution to cell therapy. Today, stem cells are precious assets for generating different types of cells derived from either natural embryonic stem (ES) cells or induced pluripotent stem (iPS) cells. The iPS technology can revolutionize the future of clinics by offering personalized medicine, which will provide the future treatment for curing untreatable diseases. Although iPS cell therapy is now at its infancy, promising research has motivated scientists to pursue this therapeutic approach...
2018: Journal of Stem Cells & Regenerative Medicine
https://www.readbyqxmd.com/read/30010246/a-new-genetic-tool-to-improve-immune-compromised-mouse-models-derivation-and-crispr-cas9-mediated-targeting-of-nrg-embryonic-stem-cell-lines
#3
Javier Martin Gonzalez, Aurélie Baudet, Sahar Abelechian, Kasper Bonderup, Teresa d'Altri, Bo Porse, Cord Brakebusch, Gunnar Juliusson, Jörg Cammenga
Development of human hematopoietic stem cells and differentiation of embryonic stem (ES) cells/induced pluripotent stem (iPS) cells to hematopoietic stem cells are poorly understood. NOD (Non-obese diabetic)-derived mouse strains, such as NSG (NOD-Scid-il2Rg) or NRG (NOD-Rag1-il2Rg), are the best available models for studying the function of fetal and adult human hematopoietic cells as well as ES/iPS cell-derived hematopoietic stem cells. Unfortunately, engraftment of human hematopoietic stem cells is very variable in these models...
July 16, 2018: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/30009512/rapid-creation-system-of-morphologically-and-functionally-communicative-three-dimensional-cell-dense-tissue-by-centrifugation
#4
Yuji Haraguchi, Katsuhisa Matsuura, Yuki Kagawa, Akiyuki Hasegawa, Hirotsugu Kubo, Tatsuya Shimizu
This study reports a rapid fabrication system of a morphologically and functionally communicative 3-D cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37°C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 µm-thickness were created at 1.5 h [centrifugation (80 x g, 37°C) for 30 min and tissue harvest for 1 h]...
July 16, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/30009216/small-molecule-based-retinal-differentiation-of-human-embryonic-stem-cells-and-induced-pluripotent-stem-cells
#5
Jie Zhu, Deepak A Lamba
Retinal degeneration leads to loss of light-sensing photoreceptors eventually resulting in vision impairment and impose a heavy burden on both patients and the society. Currently available treatment options are very limited and mainly palliative. Ever since the discovery of human pluripotent stem cell technologies, cell replacement therapy has become a promising therapeutic strategy for these patients and may help restore visual function. Reproducibly generating enriched retinal cells including retinal progenitors and differentiated retinal neurons such as photoreceptors using human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in a dish is an essential first step for developing stem cell-based therapies...
June 20, 2018: Bio-protocol
https://www.readbyqxmd.com/read/30003430/transplantation-of-human-induced-pluripotent-stem-cell-derived-neural-precursors-into-early-stage-zebrafish-embryos
#6
J Strnadel, H Wang, C Carromeu, A Miyanohara, K Fujimura, E Blahovcova, V Nosal, H Skovierova, R Klemke, E Halasova
Induced pluripotent stem cells (iPS cells) generated from somatic cells through reprogramming hold great promises for regenerative medicine. However, how reprogrammed cells survive, behave in vivo, and interact with host cells after transplantation still remains to be addressed. There is a significant need for animal models that allow in vivo tracking of transplanted cells in real time. In this regard, the zebrafish, a tropical freshwater fish, provides significant advantage as it is optically transparent and can be imaged in high resolution using confocal microscopy...
July 12, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/30003355/frequencies-of-human-leukocyte-antigen-alleles-and-haplotypes-among-japanese-patients-with-age-related-macular-degeneration
#7
Seiji Takagi, Michiko Mandai, Yasuhiko Hirami, Sunao Sugita, Masayo Takahashi, Yasuo Kurimoto
PURPOSE: Stem cell therapy is a potential treatment for retinal disorders. We are currently exploring treating HLA matched patients of age-related macular degeneration (AMD) by using allogenic retinal pigment epithelium cells derived from induced pluripotent stem cells (iPS-RPE) from human leukocyte antigen (HLA) homozygote donors. The purpose of this study was to investigate the frequency of HLA class I and II alleles and haplotypes in Japanese patients with AMD. STUDY DESIGN: Cross-sectional observation clinical study...
July 12, 2018: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/30002913/erratum-author-correction-concerns-on-a-new-therapy-for-severe-heart-failure-using-cell-sheets-with-skeletal-muscle-or-myocardial-cells-from-ips-cells-in-japan
#8
https://www.readbyqxmd.com/read/29998950/-development-of-innovative-three-dimensional-tissues-and-their-application-for-pharmaceutical-assays
#9
Michiya Matsusaki
Three-dimensional (3D) cardiac myoblast tissues derived from iPS cells were constructed by cell coating technology with nanometer-sized extracellular matrix. Vascularized 3D-cardiac tissues were also fabricated by employing cardiac endothelial cells. These 3D-cardiac tissues are expected to apply to pharmaceutical assays.
2018: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
https://www.readbyqxmd.com/read/29998222/regenerative-medicine-in-the-retina-from-stem-cells-to-cell-replacement-therapy
#10
REVIEW
Julia Oswald, Petr Baranov
Following the fast pace of the growing field of stem cell research, retinal cell replacement is finally emerging as a feasible mean to be explored for clinical application. Although neuroprotective treatments are able to slow the progression of retinal degeneration caused by diseases such as age-related macular degeneration and glaucoma, they are insufficient to fully halt disease progression and unable to recover previously lost vision. Comprehensive, technological and intellectual advances over the past years, including the in vitro differentiation of retinal cells at manufacturing scale from embryonic stem (ES) cell and induced pluripotent stem (iPS) cell cultures, progress within the area of retinal disease modeling, and the first clinical trials have started to shape the way towards addressing this treatment gap and translating retinal cell replacement to the clinic...
January 2018: Therapeutic advances in ophthalmology
https://www.readbyqxmd.com/read/29995874/directed-differentiation-of-human-induced-pluripotent-stem-cells-into-mature-kidney-podocytes-and-establishment-of-a-glomerulus-chip
#11
Samira Musah, Nikolaos Dimitrakakis, Diogo M Camacho, George M Church, Donald E Ingber
Protocols have been established to direct the differentiation of human induced pluripotent stem (iPS) cells into nephron progenitor cells and organoids containing many types of kidney cells, but it has been difficult to direct the differentiation of iPS cells to form specific types of mature human kidney cells with high yield. Here, we describe a detailed protocol for the directed differentiation of human iPS cells into mature, post-mitotic kidney glomerular podocytes with high (>90%) efficiency within 26 d and under chemically defined conditions, without genetic manipulations or subpopulation selection...
July 2018: Nature Protocols
https://www.readbyqxmd.com/read/29981365/protagonist-or-antagonist-the-complex-roles-of-retinoids-in-the-regulation-of-hematopoietic-stem-cells-and-their-specification-from-pluripotent-stem-cells
#12
REVIEW
Clea S Grace, Hanna K A Mikkola, Diana R Dou, Vincenzo Calvanese, Roger E Ronn, Louise E Purton
Hematopoietic stem cells (HSCs) are multipotent cells responsible for the maintenance of the hematopoietic system throughout life. Dysregulation of the balance between HSC self-renewal, death and differentiation can have serious consequences such as myelodysplastic syndromes (MDS) or leukemia. All-trans retinoic acid (ATRA), the biologically active metabolite of vitamin A/retinoic acid (RA), has been shown to have pleiotropic effects on hematopoietic cells, enhancing HSC self-renewal whilst also increasing differentiation of more mature progenitors...
July 4, 2018: Experimental Hematology
https://www.readbyqxmd.com/read/29974744/folic-acid-derived-hydrogel-enhances-the-survival-and-promotes-therapeutic-efficacy-of-ips-cells-for-acute-myocardial-infarction
#13
Hekai Li, Jie Gao, Yuna Shang, Yongquan Hua, Min Ye, Zhimou Yang, Cai Wen Ou, Minsheng Chen
Stem cell therapy has obtained extensive consensus to be an effective method for post myocardial infarction (MI) intervention. Induced pluripotent stem (iPS) cells, which is able to differentiate into multiple cell types, have the potential to generate cardiovascular lineage cells for myocardial repair after ischemic damage, but their poor retention rate significantly hinders the therapeutic efficacy. In the present study, we developed a supramolecular hydrogel which is formed by the self-assembly of folic acid-modified peptide via a biocompatible method (glutathione reduction), and was suitable for cell encapsulation and transplantation...
July 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29947794/efficient-crispr-cas9-mediated-editing-of-trinucleotide-repeat-expansion-in-myotonic-dystrophy-patient-derived-ips-and-myogenic-cells
#14
Sumitava Dastidar, Simon Ardui, Kshitiz Singh, Debanjana Majumdar, Nisha Nair, Yanfang Fu, Deepak Reyon, Ermira Samara, Mattia F M Gerli, Arnaud F Klein, Wito De Schrijver, Jaitip Tipanee, Sara Seneca, Warut Tulalamba, Hui Wang, Yoke Chin Chai, Peter In't Veld, Denis Furling, Francesco Saverio Tedesco, Joris R Vermeesch, J Keith Joung, Marinee K Chuah, Thierry VandenDriessche
CRISPR/Cas9 is an attractive platform to potentially correct dominant genetic diseases by gene editing with unprecedented precision. In the current proof-of-principle study, we explored the use of CRISPR/Cas9 for gene-editing in myotonic dystrophy type-1 (DM1), an autosomal-dominant muscle disorder, by excising the CTG-repeat expansion in the 3'-untranslated-region (UTR) of the human myotonic dystrophy protein kinase (DMPK) gene in DM1 patient-specific induced pluripotent stem cells (DM1-iPSC), DM1-iPSC-derived myogenic cells and DM1 patient-specific myoblasts...
June 27, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29943544/-differentiation-of-human-pluripotent-stem-cells-into-red-blood-cells
#15
Sile Wang, Ning Wang, Yuanxing Cai, Huayan Wang
At present, the experimental technique to produce human red blood cells in vitro is complicated, and in order to optimize the induction steps, human pluripotent stem cells were differentiated into red blood cells through two induction steps. First, human pluripotent stem cells (including Rh negative A type umbilical cord mesenchymal stem cells (hUCMSCRh-A) and human iPS cells (hiPS)) were differentiated into CD31+ and CD34+ cells in BVF medium. PCR and flow cytometry were used to exam the expression of CD31 and CD34...
June 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29940218/cross-site-comparison-of-excitation-contraction-coupling-using-impedance-and-field-potential-recordings-in-hipsc-cardiomyocytes
#16
Corina T Bot, Krisztina Juhasz, Fabian Haeusermann, Liudmila Polonchuk, Martin Traebert, Sonja Stoelzle-Feix
INTRODUCTION: Since 2005 the S7B and E14 guidances from ICH and FDA have been in place to assess a potential drug candidate's ability to cause long QT syndrome. To refine these guidelines, the FDA proposed the Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative, where the assessment of drug effects on cardiac repolarization was one subject of investigation. Within the myocyte validation study, effects of pharmaceutical compounds on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were assessed and this article will focus on the evaluation of the proarrhythmic potential of 23 blinded drugs in four hiPSC-CM cell lines...
June 22, 2018: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/29937717/direct-reprogramming-of-adult-human-somatic-stem-cells-into-functional-neurons-using-sox2-ascl1-and-neurog2
#17
Jessica Alves de Medeiros Araújo, Markus M Hilscher, Diego Marques-Coelho, Daiane C F Golbert, Deborah A Cornelio, Silvia R Batistuzzo de Medeiros, Richardson N Leão, Marcos R Costa
Reprogramming of somatic cells into induced pluripotent stem cells (iPS) or directly into cells from a different lineage, including neurons, has revolutionized research in regenerative medicine in recent years. Mesenchymal stem cells are good candidates for lineage reprogramming and autologous transplantation, since they can be easily isolated from accessible sources in adult humans, such as bone marrow and dental tissues. Here, we demonstrate that expression of the transcription factors (TFs) SRY (sex determining region Y)-box 2 ( Sox2 ), Mammalian achaete-scute homolog 1 ( Ascl1 ), or Neurogenin 2 ( Neurog2 ) is sufficient for reprogramming human umbilical cord mesenchymal stem cells (hUCMSC) into induced neurons (iNs)...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29934906/stem-cell-based-methods-to-predict-developmental-chemical-toxicity
#18
Hiroki Takahashi, Xian-Yang Qin, Hideko Sone, Wataru Fujibuchi
Human pluripotent stem cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells, combined with sophisticated bioinformatics methods, are powerful tools to predict developmental chemical toxicity. Because cell differentiation is not necessary, these cells can facilitate cost-effective assays, thus providing a practical system for the toxicity assessment of various types of chemicals. Here we describe how to apply machine learning techniques to different types of data, such as qRT-PCRs, gene networks, and molecular descriptors, for toxic chemicals, as well as how to integrate these data to predict toxicity categories...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29932984/grafted-miniature-swine-neural-stem-cells-of-early-embryonic-mesencephalic-neuroepithelial-origin-can-repair-the-damaged-neural-circuitry-of-parkinson-s-disease-model-rats
#19
Yutaka Mine, Toshihiko Momiyama, Takuro Hayashi, Takeshi Kawase
Although recent progress in the use of human iPS cell-derived midbrain dopaminergic progenitors is remarkable, alternatives are essential in the strategies of treatment of basal-ganglia-related diseases. Attention has been focused on neural stem cells (NSCs) as one of the possible candidates of donor material for neural transplantation, because of their multipotency and self-renewal characteristics. In the present study, miniature-swine (mini-swine) mesencephalic neuroepithelial stem cells (M-NESCs) of embryonic 17 and 18 days grafted in the parkinsonian rat striatum were assessed immunohistochemically, behaviourally and electrophysiologically to confirm their feasibility for the neural xenografting as a donor material...
June 19, 2018: Neuroscience
https://www.readbyqxmd.com/read/29920752/effects-of-muscarinic-acetylcholine-receptor-stimulation-on-the-differentiation-of-mouse-induced-pluripotent-stem-cells-into-neural-progenitor-cells
#20
Toshiaki Ishizuka, Ayako Ozawa, Mieko Katsuura, Sayaka Nomura, Yasushi Satoh
Muscarinic acetylcholine receptors (mAchRs), which are expressed in various embryonic cells, may regulate neuronal differentiation. In the present study, we examined the effects of mAchR stimulation on the differentiation of mouse induced pluripotent stem (iPS) cells into neural progenitor cells (NPCs). Mouse iPS cells were cultured on ultra-low attachment dishes to induce embryoid body (EB) formation. All-trans retinoic acid (ATRA, 3 µM) and/ or pilocarpine (10 or 100 µM), a mAchR agonist, were added to EB cultures for 4 days, following which the EBs were cultured on gelatin-coated plates for 7 days...
June 19, 2018: Clinical and Experimental Pharmacology & Physiology
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