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Lactobacillus reuteri

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https://www.readbyqxmd.com/read/27878162/asymmetric-chemoenzymatic-synthesis-of-1-3-diols-and-2-4-disubstituted-aryloxetanes-by-using-whole-cell-biocatalysts
#1
Paola Vitale, Filippo Maria Perna, Gennaro Agrimi, Antonio Scilimati, Antonio Salomone, Cosimo Cardellicchio, Vito Capriati
Regio- and stereo-selective reduction of substituted 1,3-aryldiketones, investigated in the presence of different whole cell microorganisms, was found to afford β-hydroxyketones or 1,3-diols in very good yields (up to 95%) and enantiomeric excesses (up to 96%). The enantiomerically enriched aldols, obtained with the opposite stereo-preference by baker's yeast and Lactobacillus reuteri DSM 20016 bioreduction, could then be diastereoselectively transformed into optically active syn- or anti-1,3-diols by a careful choice of the chemical reducing agent (diastereomeric ratio up to 98 : 2)...
November 23, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27873545/presence-of-lactobacillus-reuteri-in-saliva-coincide-with-higher-salivary-iga-in-young-adults-after-intake-of-probiotic-lozenges
#2
G Braathen, V Ingildsen, S Twetman, D Ericson, M R Jørgensen
The aim of this study was to compare the concentration of salivary immunoglobulin A (IgA) and the selected interleukins (IL)-1β, IL-6, IL-8 and IL-10 in young individuals with presence and non-presence of Lactobacillus reuteri in saliva after a three-week intervention with probiotic lozenges. The study group consisted of 47 healthy individuals aged 18-32 years with no clinical signs of oral inflammation. In a randomised, double-blind, placebo-controlled, cross-over trial participants ingested two lozenges per day containing two strains of the probiotic bacterium L...
November 22, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27867805/isolation-of-lactic-acid-bacteria-bound-to-the-porcine-intestinal-mucosa-and-an-analysis-of-their-moonlighting-adhesins
#3
Hideki Kinoshita, Satoko Ohuchi, Kensuke Arakawa, Masamichi Watanabe, Haruki Kitazawa, Tadao Saito
The adhesion of lactic acid bacteria (LAB) to the intestinal mucosa is one of the criteria in selecting for probiotics. Eighteen LAB were isolated from porcine intestinal mucin (PIM): ten strains of Lactobacillus, six strains of Weissella, and two strains of Streptococcus. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for phosphate-buffered saline (PBS) extracts from the LAB, many bands were detected in half of the samples, while a few and/or no clear bands were detected in the other half...
2016: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/27855715/erratum-to-antiviral-activity-of-lactobacillus-reuteri-protectis-against-coxsackievirus-a-and-enterovirus-71-infection-in-human-skeletal-muscle-and-colon-cell-lines
#4
Lei Yin Emily Ang, Horng Khit Issac Too, Eng Lee Tan, Tak-Kwong Vincent Chow, Lynette Pei-Chi Shek, Elizabeth Huiwen Tham, Sylvie Alonso
No abstract text is available yet for this article.
November 17, 2016: Virology Journal
https://www.readbyqxmd.com/read/27845560/lactic-acid-bacteria-isolated-from-poultry-protect-the-intestinal-epithelial-cells-of-chickens-from-in-vitro-wheat-germ-agglutinin-induced-cytotoxicity
#5
J D Babot, E Argañaraz Martínez, M J Lorenzo-Pisarello, M C Apella, A Perez Chaia
1. Poultry fed on wheat-based diets regularly ingest wheat germ agglutinin (WGA) that has toxic effects in vitro on intestinal epithelial cells (IEC) obtained from 14-d-old broilers. Cytotoxicity and the potential role of 14 intestinal bacterial strains in the removal of bound lectins in epithelial cell cultures was investigated. 2. Cytotoxicity was dependent on time and lectin concentration; the lethal dose (LD50) was 8.36 µg/ml for IEC exposed for 2 h to WGA. 2. Complementary sugars to WGA were detected on the surface of one Enterococcus and 9 Lactobacillus strains isolated from poultry...
November 15, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27834122/metabolic-engineering-for-probiotics-and-their-genome-wide-expression-profiling
#6
Ruby Yadav, Puneet Kumar Singh, Pratyoosh Shukla
Probiotic supplements in food industry have attracted a lot of attention and shown a remarkable growth in this field. Metabolic engineering (ME) approaches enable understanding their mechanism of action and increases possibility of designing probiotic strains with desired functions. Probiotic microorganisms generally referred as industrially important lactic acid bacteria (LAB) which are involved in fermenting dairy products, food, beverages and produces lactic acid as final product. A number of illustrations of metabolic engineering approaches in industrial probiotic bacteria have been described in this review including Transcriptomic studies of Lactobacillus reuteri and improvement in exopolysaccharide (EPS) biosynthesis yield in Lactobacillus casei LC2W...
November 11, 2016: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/27829124/crosslinked-cryostructured-lactobacillus-reuteri-monoliths-for-production-of-3-hydroxypropionaldehyde-3-hydroxypropionic-acid-and-1-3-propanediol-from-glycerol
#7
Oksana Zaushitsyna, Tarek Dishisha, Rajni Hatti-Kaul, Bo Mattiasson
Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity...
November 6, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27826653/orally-administered-multispecies-probiotic-formulations-to-prevent-uro-genital-infections-a-randomized-placebo-controlled-pilot-study
#8
Valerio Mezzasalma, Enrico Manfrini, Emanuele Ferri, Marco Boccarusso, Patrizia Di Gennaro, Irene Schiano, Angela Michelotti, Massimo Labra
PURPOSE: The aim of this study was to evaluate in the vagina of 60 pre-menopausal women the detection of orally administered multispecies probiotic formulations showing anti-microbial properties in test in vitro. METHODS: A randomized, double-blind, three-arm parallel pilot study was carried out on 60 pre-menopausal women. Subjects were randomly divided in three groups (F_1, F_2, F_3). Each group received a daily oral administration of probiotic mixtures (for 14 days and at the day 21, 7 days after the wash-out) containing: Lactobacillus acidophilus and Lactobacillus reuteri (F_1), or Lactobacillus plantarum, Lactobacillus rhamnosus and Bifidobacterium animalis subsp...
November 9, 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/27825953/microbial-lysate-upregulates-host-oxytocin
#9
Bernard J Varian, Theofilos Poutahidis, Brett T DiBenedictis, Tatiana Levkovich, Yassin Ibrahim, Eliska Didyk, Lana Shikhman, Harry K Cheung, Alexandros Hardas, Catherine E Ricciardi, Kumaran Kolandaivelu, Alexa H Veenema, Eric J Alm, Susan E Erdman
Neuropeptide hormone oxytocin has roles in social bonding, energy metabolism, and wound healing contributing to good physical, mental and social health. It was previously shown that feeding of a human commensal microbe Lactobacillus reuteri (L. reuteri) is sufficient to up-regulate endogenous oxytocin levels and improve wound healing capacity in mice. Here we show that oral L. reuteri-induced skin wound repair benefits extend to human subjects. Further, dietary supplementation with a sterile lysate of this microbe alone is sufficient to boost systemic oxytocin levels and improve wound repair capacity...
November 5, 2016: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/27811108/draft-genome-sequences-of-lactobacillus-animalis-strain-p38-and-lactobacillus-reuteri-strain-p43-isolated-from-chicken-cecum
#10
Morvarid Rezvani, Mary Mendoza, Matthew D Koci, Caitlyn Daron, Josh Levy, Hosni M Hassan
Here, we present the genome sequence of Lactobacillus animalis strain P38 and Lactobacillus reuteri strain P43, both isolated from the cecum content of a 4-week old chicken fed a diet supplemented with the prebiotic β(1-4)galacto-oligosaccharide (GOS). These indigenous Lactobacillus isolates are potential probiotic organisms for poultry.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27809987/in-vitro-inhibition-of-eimeria-tenella-sporozoite-invasion-into-host-cells-by-probiotics
#11
S Hessenberger, G Schatzmayr, K Teichmann
The aim was to study the effects of probiotics isolated from the intestinal tract of livestock animals on Eimeria tenella invasion into Madin-Darby bovine kidney (MDBK) cells in vitro. E. tenella sporozoites were purified and labeled with 5(6)-carboxyfluorescein diacetate N-succinimidyl ester before seeding on cell cultures, and invasion was evaluated by fluorescence microscopy. Two protocols (A and B) were used. In protocol A, Enterococcus faecium # 589 or Lactobacillus salivarius subsp. salivarius # 505 were added together with sporozoites to MDBK cell cultures and invasion was evaluated after incubation for approximately 20h...
October 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27801835/dietary-gluten-induced-gut-dysbiosis-is-accompanied-by-selective-upregulation-of-micrornas-with-intestinal-tight-junction-and-bacteria-binding-motifs-in-rhesus-macaque-model-of-celiac-disease
#12
Mahesh Mohan, Cheryl-Emiliane T Chow, Caitlin N Ryan, Luisa S Chan, Jason Dufour, Pyone P Aye, James Blanchard, Charles P Moehs, Karol Sestak
The composition of the gut microbiome reflects the overall health status of the host. In this study, stool samples representing the gut microbiomes from 6 gluten-sensitive (GS) captive juvenile rhesus macaques were compared with those from 6 healthy, age- and diet-matched peers. A total of 48 samples representing both groups were studied using V4 16S rRNA gene DNA analysis. Samples from GS macaques were further characterized based on type of diet administered: conventional monkey chow, i.e., wheat gluten-containing diet (GD), gluten-free diet (GFD), barley gluten-derived diet (BOMI) and reduced gluten barley-derived diet (RGB)...
October 28, 2016: Nutrients
https://www.readbyqxmd.com/read/27793228/microbes-and-oxytocin-benefits-for-host-physiology-and-behavior
#13
S E Erdman, T Poutahidis
It is now understood that gut bacteria exert effects beyond the local boundaries of the gastrointestinal tract to include distant tissues and overall health. Prototype probiotic bacterium Lactobacillus reuteri has been found to upregulate hormone oxytocin and systemic immune responses to achieve a wide array of health benefits involving wound healing, mental health, metabolism, and myoskeletal maintenance. Together these display that the gut microbiome and host animal interact via immune-endocrine-brain signaling networks...
2016: International Review of Neurobiology
https://www.readbyqxmd.com/read/27762430/antimicrobial-activity-of-lactobacillus-strains-of-chicken-origin-against-bacterial-pathogenss
#14
Marta Dec, Andrzej Puchalski, Anna Nowaczek, Andrzej Wernicki
This study was conducted to identify and evaluate the antimicrobial activity of some Lactobacillus isolates of chicken origin. Among 90 isolates 14 Lactobacillus species were distinguished using MALDI-TOF mass spectrometry and 16S-ARDRA. The dominant species was L. salivarius (34.4%), followed by L. johnsonii (23.3%), L. crispatus (13.3%) and L. reuteri (11.1%). All lactobacilli were screened for antimicrobial activity against wild-type strains of Salmonella enterica, Escherichia coli, and Clostridium perfringens...
March 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/27757509/changes-in-the-diversity-and-composition-of-gut-microbiota-of-weaned-piglets-after-oral-administration-of-lactobacillus-or-an-antibiotic
#15
Dongyan Zhang, Haifeng Ji, Hui Liu, Sixin Wang, Jing Wang, Yamin Wang
The gut microbiota plays important roles in the health and well-being of animals, and high-throughput sequencing facilitates exploration of microbial populations in the animal gut. However, previous studies have focused on fecal samples instead of the gastrointestinal tract. In this study, we compared the microbiota diversity and composition of intestinal contents of weaned piglets treated with Lactobacillus reuteri or chlortetracycline (aureomycin) using high-throughput sequencing. Nine weaned piglets were randomly divided into three groups and supplemented with L...
October 19, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27752783/techno-functional-differentiation-of-two-vitamin-b12-producing-lactobacillus-plantarum-strains-an-elucidation-for-diverse-future-use
#16
Bharat Bhushan, S K Tomar, Arun Chauhan
An appropriate selection of Lactobacillus strain (probiotic/starter/functional) on the basis of its techno-functional characteristics is required before developing a novel fermented functional food. We compared vitamin B12 (B12, cobalamin) producing Lactobacillus plantarum isolates, BHM10 and BCF20, for functional (vitamin over-production, genomic insight to B12 structural genes, and probiotic attributes) and technological [milks (skim and soy) fermentation and B12 bio-fortification] characteristics. Addition of B12 precursors (5-amonolevulinate and dimethylbenzimidazole) to cobalamin-free fermentation medium increased vitamin production in BHM10, BCF20, and DSM20016 (a positive standard) by 3...
October 17, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27748434/structural-determinants-of-alternating-%C3%AE-1%C3%A2-%C3%A2-%C3%A2-4-and-%C3%AE-1%C3%A2-%C3%A2-%C3%A2-6-linkage-specificity-in-reuteransucrase-of-lactobacillus-reuteri
#17
Xiangfeng Meng, Tjaard Pijning, Justyna M Dobruchowska, Huifang Yin, Gerrit J Gerwig, Lubbert Dijkhuizen
The glucansucrase GTFA of Lactobacillus reuteri 121 produces an α-glucan (reuteran) with a large amount of alternating (α1 → 4) and (α1 → 6) linkages. The mechanism of alternating linkage formation by this reuteransucrase has remained unclear. GTFO of the probiotic bacterium Lactobacillus reuteri ATCC 55730 shows a high sequence similarity (80%) with GTFA of L. reuteri 121; it also synthesizes an α-glucan with (α1 → 4) and (α1 → 6) linkages, but with a clearly different ratio compared to GTFA...
October 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27720582/lactobacillus-reuteri-dsm-17938-plus-vitamin-d3-as-ancillary-treatment-in-allergic-children-with-asthma
#18
Michele Miraglia Del Giudice, Nunzia Maiello, Annalisa Allegorico, Laura Iavarazzo, Michele Capasso, Carlo Capristo, Giorgio Ciprandi
No abstract text is available yet for this article.
October 6, 2016: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/27681929/biofilm-forming-lactobacillus-new-challenges-for-the-development-of-probiotics
#19
REVIEW
María José Salas-Jara, Alejandra Ilabaca, Marco Vega, Apolinaria García
Probiotics are live bacteria, generally administered in food, conferring beneficial effects to the host because they help to prevent or treat diseases, the majority of which are gastrointestinal. Numerous investigations have verified the beneficial effect of probiotic strains in biofilm form, including increased resistance to temperature, gastric pH and mechanical forces to that of their planktonic counterparts. In addition, the development of new encapsulation technologies, which have exploited the properties of biofilms in the creation of double coated capsules, has given origin to fourth generation probiotics...
September 20, 2016: Microorganisms
https://www.readbyqxmd.com/read/27641253/synthesis-of-taste-active-%C3%AE-glutamyl-dipeptides-during-sourdough-fermentation-by-lactobacillus-reuteri
#20
Cindy J Zhao, Michael G Gänzle
This study aimed to assess whether peptides influence the taste of sourdough bread. γ-Glutamyl dipeptides with known kokumi taste threshold, namely γ-Glu-Glu, γ-Glu-Leu, γ-Glu-Ile, γ-Glu-Phe, γ-Glu-Met, and γ-Glu-Val, were identified in sourdough by liquid chromatography-tandem mass spectrometry in MRM mode. γ-Glutamyl dipeptides were found in higher concentrations in sourdough fermented with Lactobacillus reuteri when compared to the chemically acidified controls. Proteolysis was an important factor for generation of γ-glutamyl dipeptides...
October 12, 2016: Journal of Agricultural and Food Chemistry
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