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https://www.readbyqxmd.com/read/27814583/3d-culture-of-mouse-gastric-stem-cells-using-porous-microcarriers
#1
Razan Alkhatib, Ali Hilal-Alnaqbi, Mariam Naciri, Rasoul Al-Majmaie, Prashanth Saseedharan, Sherif M Karam, Mohamed Al-Rubeai
The lining epithelium of the stomach includes multipotent stem cells which undergo proliferation and migration-associated differentiation. These cells give rise to multiple cell lineages that produce mucus, acid, pepsinogen and various hormones/peptides. A 3D culture for stem cells would facilitate identification of the factors that control proliferation and/or differentiation programs. Here, we report on the use of disk-like ImmobaSil HD silicone-rubber matrix based microcarriers that are permeable to oxygen and reduce the creation of toxic environment within the center of the microcarrierd for culturing the mouse gastric stem (mGS) cells...
January 1, 2017: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/27413749/assessment-of-tree-leaves-flakes-mixed-with-crude-glycerol-as-a-bioenergy-source
#2
Ali Hilal-AlNaqbi, Salah B Al-Omari, Mohamed Y E Selim
The gasification and combustion of dry tree leaves and the cogasification of dry tree leaves soaking crude glycerol were studied experimentally. An updraft fixed bed gasification and combustion system was built. The operation was conducted at different air to fuel ratios. Results show more stable combustion and more effective heat transfer to furnace walls for the cases when tree leaves flakes are mixed with 20 percent (on mass basis) of crude glycerol, as compared with the case when only dry tree leaves are used as fuel...
2016: BioMed Research International
https://www.readbyqxmd.com/read/24967035/prevalence-and-correlates-of-low-back-pain-in-primary-care-what-are-the-contributing-factors-in-a-rapidly-developing-country
#3
Abdulbari Bener, Elnour Elnaeim Dafeeah, Khalid Alnaqbi
STUDY DESIGN: Cross-sectional. PURPOSE: The purpose of the study was to determine the prevalence of low back pain (LBP) in the primary care setting with emphasis on the socio-demographic contributing factors and impact of LBP on lifestyle habits. OVERVIEW OF LITERATURE: LBP is one of the most common medical conditions seen in the Primary Health Care Clinic. METHODS: A representative sample of 2,600 patients were approached and 1,829 subjects agreed to participate in this study (70...
June 2014: Asian Spine Journal
https://www.readbyqxmd.com/read/24931958/analysis-of-the-effect-of-the-oral-contraceptive-pill-on-clinical-outcomes-in-women-with-ankylosing-spondylitis
#4
Dharini Mahendira, Arane Thavaneswaran, Adele Carty, Nigil Haroon, Ammepa Anton, Laura Passalent, Khalid A Alnaqbi, Laurie Savage, Elin Aslanyan, Robert D Inman
OBJECTIVE: There are unexplained sex-specific changes in the clinical expression of ankylosing spondylitis (AS). We sought to examine the potential effect of exogenous estrogen in the form of oral contraceptive pills (OCP) on AS initiation and severity. METHODS: This cross-sectional study consisted of women with AS from the membership of the Spondylitis Association of America. Measures of disease severity included use of biological agents and hip replacement surgery, while Bath AS Functional Index (BASFI) scores served as a surrogate marker of disability...
July 2014: Journal of Rheumatology
https://www.readbyqxmd.com/read/24870372/rf-microalgal-lipid-content-characterization
#5
Mahmoud Al Ahmad, Sulaiman Al-Zuhair, Hanifa Taher, Ali Hilal-Alnaqbi
Most conventional techniques for the determination of microalgae lipid content are time consuming and in most cases are indirect and require excessive sample preparations. This work presents a new technique that utilizes radio frequency (RF) for rapid lipid quantification, without the need for sample preparation. Tests showed that a shift in the resonance frequency of a RF open-ended coaxial resonator and a gradual increase in its resonance magnitude may occur as the lipids content of microalgae cells increases...
2014: Scientific Reports
https://www.readbyqxmd.com/read/24164246/effect-of-membranes-on-oxygen-transfer-rate-and-consumption-within-a-newly-developed-three-compartment-bioartificial-liver-device-advanced-experimental-and-theoretical-studies
#6
Ali Hilal-Alnaqbi, Abdel-Hamid I Mourad, Basem F Yousef
A mathematical model is developed to predict oxygen transfer in the fiber-in-fiber (FIF) bioartificial liver device. The model parameters are taken from the constructed and tested FIF modules. We extended the Krogh cylinder model by including one more zone for oxygen transfer. Cellular oxygen uptake was based on Michaelis-Menten kinetics. The effect of varying a number of important model parameters is investigated, including (1) oxygen partial pressure at the inlet, (2) the hydraulic permeability of compartment B (cell region), (3) the hydraulic permeability of the inner membrane, and (4) the oxygen diffusivity of the outer membrane...
May 2014: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/23996291/development-sensibility-and-reliability-of-the-toronto-axial-spondyloarthritis-questionnaire-in-inflammatory-bowel-disease
#7
Khalid A Alnaqbi, Zahi Touma, Laura Passalent, Sindhu R Johnson, George A Tomlinson, Adele Carty, Robert D Inman
OBJECTIVE: There is an unacceptable delay in the diagnosis of axial spondyloarthritis (axSpA) in its early stages among patients at high risk, in particular those with inflammatory bowel disease (IBD). Our objectives were to develop a sensible and reliable questionnaire to identify undetected axSpA among patients with IBD. METHODS: Literature was reviewed for item generation in the Toronto axSpA Questionnaire on IBD (TASQ-IBD). Sensibility of the questionnaire was assessed among healthcare professionals and patients...
October 2013: Journal of Rheumatology
https://www.readbyqxmd.com/read/23988710/experimental-evaluation-and-theoretical-modeling-of-oxygen-transfer-rate-for-the-newly-developed-hollow-fiber-bioreactor-with-three-compartments
#8
Ali Hilal-Alnaqbi, Abdel-Hamid I Mourad, Basem F Yousef, John D S Gaylor
Bioartificial liver support provides a bridge to transplantation which is at present the only proven specific treatment for acute liver failure. In this paper, a novel multi-coaxial hollow fiber bioreactor so-called "Fibre-in-Fibre FIF Bioartificial liver device" with three compartments is experimentally and mathematically studied. The mathematical model in this paper is an extension of Krogh cylinder model for hollow fibre devices by including one more zone for oxygen transfer, i.e. oxygenation compartment...
2013: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/23799711/an-epidemiologic-analysis-of-low-back-pain-in-primary-care-a-hot-humid-country-and-global-comparison
#9
Abdulbari Bener, Elnour E Dafeeah, Khalid Alnaqbi, Omar Falah, Taha Aljuhaisi, Alhassan Sadeeq, Shehryar Khan, Josia Schlogl
BACKGROUND: Low back pain is one of the most common conditions for which patients seek medical care. AIM: The aim of the study was to study the epidemiology of low back pain in primary care setting with emphasis on frequency, sociodemographic factors, and impact of low back pain on lifestyle habits. SUBJECTS AND METHODS: This is a cross-sectional study. A representative sample of 2742 patients was approached and 2180 subjects agreed to participate in this study (79...
July 1, 2013: Journal of Primary Care & Community Health
https://www.readbyqxmd.com/read/23701045/growth-metabolic-activity-and-productivity-of-immobilized-and-freely-suspended-cho-cells-in-perfusion-culture
#10
Ali Hilal-Alnaqbi, Alan Y C Hu, Zhibing Zhang, Mohamed Al-Rubeai
Chinese hamster ovary (CHO) cells producing β-galactosidase (β-gal) were successfully cultured on silicone-based porous microcarriers (ImmobaSil FS) in a 1 L stirred-tank perfusion bioreactor. We studied the growth, metabolism, and productivity of free and immobilized cells to understand cellular activity in immobilized conditions. CHO cells attached to ImmobaSil FS significantly better than to other microcarriers. Scanning electron microscope images showed that the CHO cells thoroughly colonized the porous surfaces of the ImmobaSil FS, exhibiting a spherical morphology with microvilli that extended to anchorage cells on the silicone surface...
July 2013: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/22644089/rituximab-in-a-patient-with-ankylosing-spondylitis-with-demyelinating-disease-a-case-report-and-review-of-the-literature
#11
REVIEW
Mohammed A Omair, Khalid A Alnaqbi, Peter Lee
Tumor necrosis factor inhibitors (TNFI) are novel therapies used to treat ankylosing spondylitis (AS). Demyelinating disease is a rare but serious complication of TNFI. We report a patient with a case of AS who developed demyelinating disease secondary to TNFI and was treated successfully with rituximab.
August 2012: Clinical Rheumatology
https://www.readbyqxmd.com/read/18043824/primary-umbilical-adenocarcinoma
#12
K A Y Alnaqbi, S Joshi, S Ghazal-Aswad, F M Abu Zidan
Primary umbilical tumours are extremely rare. We report primary serous adenocarcinoma arising from the coelomic mesothelium of the hernial sac. A 60-year-old woman presented with an umbilical swelling of six months duration that became painful in the last three days. Examination revealed a tender umbilical swelling diagnosed as obstructive hernia that needed surgery. When dissecting the sac during surgery, a small subcutaneous abscess was encountered. The sac contained an omentum with a hard nodule at the surface which was excised...
December 2007: Singapore Medical Journal
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