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Yuli Ekowati, Anne D van Diepeningen, Giuliana Ferrero, Maria D Kennedy, Ana Maria de Roda Husman, Franciska M Schets
The density of fungal contamination and the fungal diversity in an indoor swimming pool facility were assessed. A total of 16 surface samples and 6 water samples were analysed by using a combination of different (semi-) selective culture media. Isolated fungal colonies were identified to the genus or species level by sequencing of the internal transcribed spacer (ITS). The highest fungal counts in water and on surfaces were in the recreational pool (17CFU/100mL) and on a flexibeam (5.8CFU/cm(2)), respectively as compared with low counts (<0...
July 10, 2017: International Journal of Hygiene and Environmental Health
Charlotte Berthelot, Yoann Perrin, Corinne Leyval, Damien Blaudez
Dark septate endophytes (DSEs) are melanin-enriched ascomycetous fungi that are abundant in stressed environments. However, little is known about their physiology and metabolism, and DSE genes have not been functionally characterized yet. Therefore developing molecular genetic tools to investigate the biological function of genes of interest in DSEs is of major significance. We investigated Agrobacterium tumefaciens-mediated transformation (ATMT) efficiency in eight DSE strains belonging to Cadophora sp., Cadophora malorum, Leptodontidium sp...
August 2017: Fungal Biology
Charlotte Berthelot, Damien Blaudez, Corinne Leyval
Dark septate endophytes (DSEs) are abundant in stressful environments, including trace element- (TE-) enriched soils. However, knowledge about the effects of DSEs on plant growth in such soils is poor compared to the well-known mycorrhizal fungi. The aim of this work was to evaluate the effects of three DSE strains isolated from TE-contaminated soils on the growth and mineral nutrition of Betula pendula and Populus tremula x alba grown on two contrasting TE-polluted soils. The three DSEs evenly colonized the two plant species in both soils...
May 19, 2017: International Journal of Phytoremediation
Ying-Ke Zhou, Xiao-Ye Shen, Cheng-Lin Hou
An important and useful bamboo species, fishscale bamboo (Phyllostachys heteroclada Oliver), is broadly distributed in Southeast China and has multiple purposes, including uses in cuisine, weaving, Chinese medicine and ecological protection. However, no previous studies have focused on the endophytes of this plant. In our article, a total of 127 fungal strains were first isolated from the healthy branches and leaves of common P. heteroclada. These endophytic fungi could be directly categorized into 50 morphotypes according to their culture characteristics, and their internal transcribed spacer (ITS) regions were analyzed for molecular identification...
June 2017: World Journal of Microbiology & Biotechnology
Marcela Queiroz Granato, Diego de Souza Gonçalves, Sergio Henrique Seabra, Malachy McCann, Michael Devereux, André Luis Souza Dos Santos, Lucimar Ferreira Kneipp
Phialophora verrucosa is a dematiaceous fungus able to cause chromoblastomycosis, phaeohyphomycosis and mycetoma. All these fungal diseases are extremely difficult to treat and often refractory to the current therapeutic approaches. Therefore, there is an urgent necessity to develop new antifungal agents to combat these mycoses. In this context, the aim of the present work was to investigate the effect of 1,10-phenanthroline-5,6-dione (phendione) and its metal-based derivatives [Ag(phendione)2]ClO4 = ([Ag(phendione)2](+)) and [Cu(phendione)3](ClO4)2...
2017: Frontiers in Microbiology
Zahoor Ahmed Wani, Dania Nazir Mirza, Palak Arora, Syed Riyaz-Ul-Hassan
A total of 294 fungal endophytes were isolated from the corms of Crocus sativus at two stages of crocus life cycle collected from 14 different saffron growing sites in Jammu and Kashmir (J & K) State, India. Molecular phylogeny assigned them into 36 distinct internal transcribed spacer (ITS) genotypes which spread over 19 genera. The diversity of endophytes was higher at the dormant than at the vegetative stage. The Saffron microbiome was dominated by Phialophora mustea and Cadophora malorum, both are dark septate endophytes (DSEs)...
December 2016: Fungal Biology
Aditi Chhonkar, Deepti Kataria, Swagata Tambe, Chitra S Nayak
Phaeohyphomycosis is a chronic infectious condition caused by dematiaceous fungi which usually involve the skin and subcutaneous tissue. Subcutaneous phaeohyphomycosis is characterised by papulonodules, verrucous, hyperkeratotic or ulcerated plaques, cysts, abscesses, pyogranuloma, non-healing ulcers or sinuses. In India, commonly associated genera are Exophiala, Phialophora, Cladosporium, Curvularia, Fonsecaea and Alternaria. This condition involves the presence of brown-walled hyphal structures in the dermis and epidermis...
May 2016: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
Jun-Wei He, Chuan-Xi Wang, Li Yang, Guo-Dong Chen, Dan Hu, Liang-Dong Guo, Xin-Sheng Yao, Hao Gao
A pair of new enantiomeric polyketides, (-)- and (+)-nigrosporaol A (1a and 1b), along with one related known compound, (+)-alternarienoic acid (2), were isolated from three endolichenic fungal strains, Nigrospora sphaerica (No.83-1-1-2), Alternaria alternata (No.58-8-4-1), and Phialophora sp.(No.96-1-8-1). Their structures, including the absolute configurations, were elucidated on the basis of spectroscopic methods, X-ray diffraction analysis, and the modified Mosher's method.
June 2016: Natural Product Communications
Weiwei Wu, Ruijun Zhang, Xiaowen Wang, Yinggai Song, Zhengyang Liu, Wenling Han, Ruoyu Li
Dematiaceous fungi are a large group of pathogens that can cause a wide range of diseases in both immunocompetent and immunocompromised hosts. Based on our previous finding of caspase recruitment domain-containing protein 9 (CARD9) mutations in patients with subcutaneous phaeohyphomycosis caused by Phialophora verrucosa (P. verrucosa), we further investigated the exact role of CARD9 in the pathogenesis of phaeohyphomycosis using Card9 knockout (Card9 KO) mice. We showed that Card9 KO mice are profoundly susceptible to P...
October 2016: Mycopathologia
Charlotte Berthelot, Corinne Leyval, Julie Foulon, Michel Chalot, Damien Blaudez
Numerous studies address the distribution and the diversity of dark septate endophytes (DSEs) in the literature, but little is known about their ecological role and their effect on host plants, especially in metal-polluted soils. Seven DSE strains belonging to Cadophora, Leptodontidium, Phialophora and Phialocephala were isolated from roots of poplar trees from metal-polluted sites. All strains developed on a wide range of carbohydrates, including cell-wall-related compounds. The strains evenly colonized birch, eucalyptus and ryegrass roots in re-synthesis experiments...
October 2016: FEMS Microbiology Ecology
Ana Karina Alves de Medeiros, Evelyn Lodewick, Delfien J A Bogaert, Filomeen Haerynck, Sabine Van Daele, Bart Lambrecht, Sara Bosma, Laure Vanderdonckt, Olivier Lortholary, Mélanie Migaud, Jean-Laurent Casanova, Anne Puel, Fanny Lanternier, Jo Lambert, Lieve Brochez, Melissa Dullaers
Chronic mucocutaneous or invasive fungal infections are generally the result of primary or secondary immune dysfunction. Patients with autosomal recessive CARD9 mutations are also predisposed to recurrent mucocutaneous and invasive fungal infections with Candida spp., dermatophytes (e.g., Trichophyton spp.) and phaeohyphomycetes (Exophiala spp., Phialophora verrucosa). We study a consanguineous family of Turkish origin in which three members present with distinct clinical phenotypes of chronic mucocutaneous and invasive fungal infections, ranging from chronic mucocutaneous candidiasis (CMC) in one patient, treatment-resistant cutaneous dermatophytosis and deep dermatophytosis in a second patient, to CMC with Candida encephalitis and endocrinopathy in a third patient...
April 2016: Journal of Clinical Immunology
Leandro F Moreno, J Benjamin Stielow, Michel de Vries, Vinicius A Weiss, Vania A Vicente, Sybren de Hoog
The black yeast Phialophora attae was isolated from the cuticle of tropical ant gynes. The ant-fungus association is sustained due to symbiotic evolutionary adaptations that allow fungal assimilation and tolerance of toxic compounds produced by the ant. The genome sequence of the first ant-associated fungus, P. attae, is presented here.
2015: Genome Announcements
Erick Obed Martínez-Herrera, Stefanie Arroyo-Camarena, Diana Luz Tejada-García, Carlos Francisco Porras-López, Roberto Arenas
BACKGROUND: Onychomycosis are caused by dermatophytes and Candida, but rarely by non- dermatophyte molds. These opportunistic agents are filamentous fungi found as soil and plant pathogens. OBJECTIVES: To determine the frequency of opportunistic molds in onychomycosis. METHODS: A retrospective analysis of 4,220 cases with onychomycosis, diagnosed in a 39-month period at the Institute of Dermatology and Skin surgery "Prof. Dr. Fernando A. Cordero C...
May 2015: Anais Brasileiros de Dermatologia
Akiko Takeuchi, Kazufumi Anzawa, Takashi Mochizuki, Kazuhiko Takehara, Yasuhito Hamaguchi
No abstract text is available yet for this article.
May 2015: European Journal of Dermatology: EJD
Michael Sorkin, Theodore A Kung, Kevin C Chung
Deep tissue fungal infections of the hand are exceedingly uncommon. We present a case of fungal tenosynovitis caused by Phialophora verrucosa that led to extensor tendon rupture in a patient who was on chronic immunosuppressive therapy. Indolent fungal cysts can elude clinical diagnosis until excision is performed with definitive pathologic examination. In immunocompromised patients, antifungal therapy may be warranted after cyst excision even in the absence of acute infection to prevent subsequent progression to tenosynovitis...
June 2015: Hand: Official Journal of the American Association for Hand Surgery
Pin Liang, Xiaowen Wang, Runchao Wang, Zhe Wan, Wenling Han, Ruoyu Li
Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor molecule that is critical for NF-κB activation and forms a complex with B cell lymphoma 10 and mucosa-associated lymphoid tissue lymphoma translocation gene 1 that mediates C-type lectin receptors (CLRs)-triggered intracellular signaling during antifungal immunity. However, the role of CARD9 in the host defense against Phialophora verrucosa (P. verrucosa) infection remains to be elucidated. In the present study, we investigated the functions of polymorphonuclear neutrophils (PMNs) from patients with CARD9 deficiencies against P...
June 2015: Mycopathologia
Sadia Khan, Anil Kumar, Vivek Vinod, Vivek Prabhakar, Malini Eapen, Jacob Thomas, Kavitha Dinesh, Shamsul Karim
Chromoblastomycosis is a chronic subcutaneous mycosis commonly caused by Fonsecaea, Phialophora, and Cladophialophora spp. Out of these, Fonsecaea pedrosoi is the most common etiological agent, implicated in 70%-90% of the cases reported worldwide. The histopathological diagnosis of chromoblastomycosis is based on visualization of medlar or sclerotic bodies in the tissue. These sclerotic bodies divide by planar division. Rarely, budding is seen in these sclerotic bodies. As this entity can be confused with phaeohyphomycosis, it is important to be aware of such a presentation also...
March 2015: Journal of Infection in Developing Countries
Fanny Lanternier, Seyed Alireza Mahdaviani, Elisa Barbati, Hélène Chaussade, Yatrika Koumar, Romain Levy, Blandine Denis, Anne-Sophie Brunel, Sophie Martin, Michèle Loop, Julie Peeters, Ariel de Selys, Jean Vanclaire, Christiane Vermylen, Marie-Cécile Nassogne, Olga Chatzis, Luyan Liu, Mélanie Migaud, Vincent Pedergnana, Guillaume Desoubeaux, Gregory Jouvion, Fabrice Chretien, Ilad Alavi Darazam, Alejandro A Schäffer, Mihai G Netea, Jean J De Bruycker, Louis Bernard, Jacques Reynes, Noureddine Amazrine, Laurent Abel, Dimitri Van der Linden, Tom Harrison, Capucine Picard, Olivier Lortholary, Davood Mansouri, Jean-Laurent Casanova, Anne Puel
BACKGROUND: Invasive infections of the central nervous system (CNS) or digestive tract caused by commensal fungi of the genus Candida are rare and life-threatening. The known risk factors include acquired and inherited immunodeficiencies, with patients often displaying a history of multiple infections. Cases of meningoencephalitis, colitis, or both caused by Candida species remain unexplained. OBJECTIVE: We studied 5 previously healthy children and adults with unexplained invasive disease of the CNS, digestive tract, or both caused by Candida species...
June 2015: Journal of Allergy and Clinical Immunology
Anne Bernhardt, Wolf von Bomhard, Elisabeth Antweiler, Kathrin Tintelnot
In a retrospective study, we investigated 52 formalin-fixed, paraffin-embedded (FFPE) samples from cats with histologically confirmed cutaneous and subcutaneous mycoses to determine if the pathogens could be identified by molecular methods. Aim of the study was to obtain a deep understanding of the spectrum of infectious agents, which, as we hypothesized, was not available by histopathology alone. Detection of feline and fungal DNA was achieved in 92.3% and 94.2% of the samples, respectively. Most of the subcutaneous infections in cats were caused by Alternaria spp...
February 1, 2015: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Marcela Queiroz Granato, Priscila de Araújo Massapust, Sonia Rozental, Celuta Sales Alviano, André Luis Souza dos Santos, Lucimar Ferreira Kneipp
Phialophora verrucosa is one of the etiologic agents of chromoblastomycosis, a fungal infection that affects cutaneous and subcutaneous tissues. This disease is chronic, recurrent and difficult to treat. Several studies have shown that secreted peptidases by fungi are associated with important pathophysiological processes. Herein, we have identified and partially characterized the peptidase activity secreted by P. verrucosa conidial cells. Using human serum albumin as substrate, the best hydrolysis profile was detected at extreme acidic pH (3...
April 2015: Mycopathologia
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