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https://www.readbyqxmd.com/read/28560128/identification-of-aqueous-access-residues-of-the-sodium-half-channel-in-transmembrane-helix-5-of-the-fo-a-subunit-of-propionigenium-modestum-atp-synthase
#1
Noriyo Mitome, Hiroki Sato, Taishi Tomiyama, Katsuya Shimabukuro, Takuya Matsunishi, Kohei Hamada, Toshiharu Suzuki
The Fo-a subunit of the Na(+)-transporting FoF1 ATP synthase from Propionigenium modestum plays a key role in Na(+) transport. It forms half channels that allow Na(+) to enter and leave the buried carboxyl group on Fo-c subunits. The essential Arg residue R226, which faces the carboxyl group of Fo-c subunits in the middle of transmembrane helix 5 of the Fo-a subunit, separates the cytoplasmic side and periplasmic half-channels. To elucidate contributions of other amino acid residues of transmembrane helix 5 using hybrid FoF1 (Fo from P...
2017: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/28506052/-renal-cell-carcinoma-with-retroperitoneal-metastases-and-renal-invasion-from-breast-cancer-a-case-report
#2
Go Noguchi, Yoko Saito, Taku Mitome, Susumu Umemoto, Ayano Kakazu, Tomoko Sugiyama, Teiichiro Ueki
A 67-year-old female was hospitalized with back pain. Computed tomography (CT) incidentally revealed a tumor in her left kidney tumor (33 mm) and bilateral breast tumors. She underwent a breast biopsy and was diagnosed with breast cancer (invasive lobular cancer, cT2N0M0). The renal tumor was suspected to be clear cell carcinoma, cT1aN0M0, based on contrast-enhanced CT. Surgery was considered necessary for both the breast cancer and renal tumor. First, laparoscopic radical nephrectomy was performed for the renal tumor...
April 2017: Hinyokika Kiyo. Acta Urologica Japonica
https://www.readbyqxmd.com/read/28465777/use-of-the-prostate-core-mitomic-test-in-repeated-biopsy-decision-making-real-world-assessment-of-clinical-utility-in-a-multicenter-patient-population
#3
Lorena Legisi, Elise DeSa, M Nasar Qureshi
BACKGROUND: Prostate cancer is the most common cancer diagnosed in men in developed countries. Using molecular testing may help to improve outcomes in this clinically challenging group. Since 2011, the Prostate Core Mitomic Test (PCMT), which quantifies a 3.4-kb mitochondrial DNA deletion strongly associated with prostate cancer, has been used by more than 50 urology practices accessing pathology services through our laboratory in New Jersey. However, the use of a molecular test can only be beneficial if it affects patient management and improves outcomes...
December 2016: American Health & Drug Benefits
https://www.readbyqxmd.com/read/28351193/measurement-of-serum-isoform-2-propsa-derivatives-shows-superior-accuracy-to-magnetic-resonance-imaging-in-the-diagnosis-of-prostate-cancer-in-patients-with-a-total-prostate-specific-antigen-level-of-2-10%C3%A2-ng-ml
#4
Kazuhiro Furuya, Takashi Kawahara, Masaki Narahara, Takashi Tokita, Sachi Fukui, Masashi Imano, Taku Mitome, Yusuke Ito, Koji Izumi, Kimito Osaka, Yumiko Yokomizo, Narihiko Hayashi, Hisashi Hasumi, Shintaro Nawata, Tsuyoshi Kawano, Masahiro Yao, Hiroji Uemura
OBJECTIVE: More accurate diagnostic procedures for prostate cancer are needed to avoid unnecessary biopsy due to the low specificity of prostate-specific antigen (PSA). Recent studies showed that the percentage of serum isoform [-2]proPSA (p2PSA) to free PSA (%p2PSA), the Prostate Health Index (PHI) and magnetic resonance imaging (MRI) were more accurate than PSA. The aim of this study was to test the accuracy of %p2PSA, PHI and MRI in discriminating patients with and without prostate cancer...
March 29, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/27606954/statistically-analyzed-photoresponse-of-elastically-bent-cds-nanowires-probed-by-light-compatible-in-situ-high-resolution-tem
#5
Chao Zhang, Ovidiu Cretu, Dmitry G Kvashnin, Naoyuki Kawamoto, Masanori Mitome, Xi Wang, Yoshio Bando, Pavel B Sorokin, Dmitri Golberg
We demonstrate that high resolution transmission electron microscopy (HRTEM) paired with light illumination of a sample and its electrical probing can be utilized for the in situ study of initiated photocurrents in free-standing nanowires. Morphology, phase and crystallographic information from numerous individual CdS nanowires is obtained simultaneously with photocurrent measurements. Our results indicate that elastically bent CdS nanowires possessing a wurtzite structure show statistically unchanged values of ON/OFF (photocurrent/dark current) ratios...
October 12, 2016: Nano Letters
https://www.readbyqxmd.com/read/27512015/constituent-elements-and-their-distribution-in-the-radioactive-cs-bearing-silicate-glass-microparticles-released-from-fukushima-nuclear-plant
#6
Toshihiro Kogure, Noriko Yamaguchi, Hiroyo Segawa, Hiroki Mukai, Satoko Motai, Kotone Akiyama-Hasegawa, Masanori Mitome, Toru Hara, Tsuyoshi Yaita
Microparticles of radioactive cesium (Cs)-bearing silicate glass emitted from the Fukushima Daiichi nuclear power plant were investigated mainly using state-of-the-art energy-dispersive X-ray spectroscopy in scanning transmission electron microscopes. Precise elemental maps of the particles were obtained using double silicon drift detectors with a large collection angle of X-rays, and qualitative elemental analysis was performed using high-resolution X-ray spectroscopy with a microcalorimetry detector. Beside the substantial elements (O, Si, Cl, K, Fe, Zn, Rb, Sn and Cs) as previously reported, Mn and Ba were also common, though their amounts were small...
October 2016: Microscopy
https://www.readbyqxmd.com/read/27403587/mechanistic-insights-into-c-h-oxidations-by-ruthenium-iii-pterin-complexes-impact-of-basicity-of-the-pterin-ligand-and-electron-acceptability-of-the-metal-center-on-the-transition-states
#7
Hiroumi Mitome, Tomoya Ishizuka, Hiroaki Kotani, Yoshihito Shiota, Kazunari Yoshizawa, Takahiko Kojima
A ruthenium(II) complex, [Ru(dmdmp)Cl(MeBPA)] (2) (Hdmdmp = N,N-dimethyl-6,7-dimethylpterin, MeBPA = N-methyl-N,N-bis(pyridylmethyl)amine), having a pterin derivative as a proton-accepting ligand, was synthesized and characterized. Complex 2 shows higher basicity than that of a previously reported Ru(II)-pterin complex, [Ru(dmdmp) (TPA)](+) (1) (TPA = tris(2-pyridylmethyl)amine). On the other hand, 1e(-)-oxidized species of 1 (1OX) exhibits higher electron-acceptability than that of 1e(-)-oxidized 2 (2OX). Bond dissociation enthalpies (BDE) of the two Ru(II) complexes having Hdmdmp as a ligand in proton-coupled electron transfer (PCET) to generate 1OX and 2OX were calculated to be 85 kcal mol(-1) for 1OX and 78 kcal mol(-1) for 2OX...
August 3, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27007992/amitorines-a-and-b-nitrogenous-diterpene-metabolites-of-theonella-swinhoei-isolation-structure-elucidation-and-asymmetric-synthesis
#8
Koichiro Ota, Yukiko Hamamoto, Wakiko Eda, Kenta Tamura, Akiyoshi Sawada, Ayako Hoshino, Hidemichi Mitome, Kazuo Kamaike, Hiroaki Miyaoka
Two new nitrogenous prenylbisabolanes never before found in Lithistid sponges have been isolated from Theonella swinhoei. These new diterpenes, named amitorine A (1) and amitorine B (2), containing a prenylbisabolane skeleton have been characterized by spectroscopic analyses, and the relative and absolute configurations of 1 and 2 were determined by asymmetric synthesis of both diastereomers via the common bicyclic lactone 6 intermediate.
April 22, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/26838055/internal-structure-of-cesium-bearing-radioactive-microparticles-released-from-fukushima-nuclear-power-plant
#9
Noriko Yamaguchi, Masanori Mitome, Akiyama-Hasegawa Kotone, Maki Asano, Kouji Adachi, Toshihiro Kogure
Microparticles containing substantial amounts of radiocesium collected from the ground in Fukushima were investigated mainly by transmission electron microscopy (TEM) and X-ray microanalysis with scanning TEM (STEM). Particles of around 2 μm in diameter are basically silicate glass containing Fe and Zn as transition metals, Cs, Rb and K as alkali ions, and Sn as substantial elements. These elements are homogeneously distributed in the glass except Cs which has a concentration gradient, increasing from center to surface...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26783426/bilateral-renal-lymphoma-rapid-recovery-from-an-acute-kidney-injury-after-open-renal-biopsy
#10
Taku Mitome, Kazuhiro Furuya, Masashi Imano, Kimito Osaka, Yumiko Yokomizo, Narihiko Hayashi, Noboru Nakaigawa, Shoji Yamanaka, Masahiro Yao
Renal lymphoma as an initial lesion is relatively rare. Bilateral renal lymphoma frequently presents as acute kidney injury. With systematic chemotherapy for the lymphoma, patients usually recover their kidney function. However, in the case we describe here, the patient's kidney function recovered greatly after an open renal biopsy. Here, we review and discuss this unique case.
January 2016: Clinical Case Reports
https://www.readbyqxmd.com/read/26511400/pollutant-capturing-sers-substrate-porous-boron-nitride-microfibers-with-uniform-silver-nanoparticle-decoration
#11
Pengcheng Dai, Yanming Xue, Xuebin Wang, Qunhong Weng, Chao Zhang, Xiangfen Jiang, Daiming Tang, Xi Wang, Naoyuki Kawamoto, Yusuke Ide, Masanori Mitome, Dmitri Golberg, Yoshio Bando
How to concentrate target molecules on the surface of a SERS substrate is a key problem in the practical application of SERS. Herein, we designed for the first time a pollutant capturing surface enhanced Raman spectroscopy (SERS) substrate, namely porous BN microfibers uniformly decorated with Ag nanoparticles, in which the BN microfibers adsorb pollutants, while the Ag nanoparticles provide SERS activity. This SERS substrate captures pollutants from an aqueous solution completely and accumulates them all on its surface without introducing noise signals...
December 7, 2015: Nanoscale
https://www.readbyqxmd.com/read/26508524/nanoscale-characterization-of-the-thermal-interface-resistance-of-a-heat-sink-composite-material-by-in-situ-tem
#12
Naoyuki Kawamoto, Yohei Kakefuda, Takao Mori, Kenji Hirose, Masanori Mitome, Yoshio Bando, Dmitri Golberg
We developed an original method of in situ nanoscale characterization of thermal resistance utilizing a high-resolution transmission electron microscope (HRTEM). The focused electron beam of the HRTEM was used as a contact-free heat source and a piezo-movable nanothermocouple was developed as a thermal detector. This method has a high flexibility of supplying thermal-flux directions for nano/microscale thermal conductivity analysis, and is a powerful way to probe the thermal properties of complex or composite materials...
November 20, 2015: Nanotechnology
https://www.readbyqxmd.com/read/26114583/amorphization-and-directional-crystallization-of-metals-confined-in-carbon-nanotubes-investigated-by-in-situ-transmission-electron-microscopy
#13
Dai-Ming Tang, Cui-Lan Ren, Ruitao Lv, Wan-Jing Yu, Peng-Xiang Hou, Ming-Sheng Wang, Xianlong Wei, Zhi Xu, Naoyuki Kawamoto, Yoshio Bando, Masanori Mitome, Chang Liu, Hui-Ming Cheng, Dmitri Golberg
The hollow core of a carbon nanotube (CNT) provides a unique opportunity to explore the physics, chemistry, biology, and metallurgy of different materials confined in such nanospace. Here, we investigate the nonequilibrium metallurgical processes taking place inside CNTs by in situ transmission electron microscopy using CNTs as nanoscale resistively heated crucibles having encapsulated metal nanowires/crystals in their channels. Because of nanometer size of the system and intimate contact between the CNTs and confined metals, an efficient heat transfer and high cooling rates (∼10(13) K/s) were achieved as a result of a flash bias pulse followed by system natural quenching, leading to the formation of disordered amorphous-like structures in iron, cobalt, and gold...
August 12, 2015: Nano Letters
https://www.readbyqxmd.com/read/26054411/novel-biomarkers-and-genomic-tests-in-prostate-cancer-a-critical-analysis
#14
REVIEW
S M Falzarano, M Ferro, E Bollito, E A Klein, G Carrieri, C Magi-Galluzzi
The aim of this review is to critically analyze the current state of research in selected biomarkers and genomic-based tests for prostate cancer (PCa) diagnosis, staging, prognostication, and monitoring. Although in Western societies, PCa is the most common solid malignancy and the second leading cause of cancer death in men, the vast majority of men with PCa are diagnosed with clinically localized disease. The widespread use of prostate-specific antigen (PSA) testing, on one hand, has resulted in earlier PCa detection at a potentially more curable stage, but on the other hand has led to an increase in the rate of negative biopsies, as well as overdetection and overtreatment of potentially indolent tumors that would not have become life-threatening to a patient...
September 2015: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/26031583/change-in-glomerular-volume-and-its-clinicopathological-impact-after-kidney-transplantation
#15
Akimitsu Kobayashi, Izumi Yamamoto, Haruki Katsumata, Takafumi Yamakawa, Aki Mafune, Yasuyuki Nakada, Kentaro Koike, Jun Mitome, Jun Miki, Hiroki Yamada, Yudo Tanno, Ichiro Ohkido, Nobuo Tsuboi, Keitaro Yokoyama, Hiroyasu Yamamoto, Takashi Yokoo
BACKGROUND: Both immunological and non-immunological etiologies affect graft function after kidney transplantation, including acute rejection, calcineurin inhibitor toxicity, and a recurrence of glomerulonephritis. Glomerular enlargement or glomerular sclerosis due to glomerular hyperfiltration related to increased renal blood flow is another cause. Although the glomerular volume in baseline biopsies predicts late allograft function, the relationship between allograft function and the annual changes in glomerular volume after kidney transplantation are unclear...
July 2015: Nephrology
https://www.readbyqxmd.com/read/26024771/differences-in-tissue-proliferation-and-maturation-between-matrix2-and-bare-platinum-coil-embolization-in-experimental-swine-aneurysms
#16
Yumiko Mitome-Mishima, Hidenori Oishi, Munetaka Yamamoto, Kenji Yatomi, Senshu Nonaka, Nobukazu Miyamoto, Takao Urabe, Hajime Arai
BACKGROUND AND PURPOSE: Recanalization of post-embolization cerebral aneurysms remains a serious problem that influences treatment outcomes. Matrix2 is a bioactive, bio-absorbable, detachable coil that was developed to reduce the risk of recanalization. We examined the short-term efficacy of the Matrix2 coil system, and evaluated the temporal profile of tissue proliferation in a swine experimental aneurysm model compared with the bare platinum (BP) coil. MATERIALS AND METHODS: Thirty-six experimental aneurysms were created in 18 swine...
February 2016: Journal of Neuroradiology. Journal de Neuroradiologie
https://www.readbyqxmd.com/read/25624964/mucinous-cystadenoma-of-a-horseshoe-kidney-a-case-report-and-literature-review
#17
Taku Mitome, Masahiro Yao, Naoko Udaka, Shusei Fusayasu, Koji Izumi, Kimito Osaka, Narihiko Hayashi, Noboru Nakaigawa, Yoji Nagashima, Yoshinobu Kubota
A 45-year-old man complained of a palpable mass in his left abdomen. Computed tomography showed a horseshoe kidney with a Bosniak type II complicated cyst from a left segment. Three years after his initial examination, due to the growing cystic lesion and the compression imposed on the urinary collecting system and surrounding organs, we performed a left heminephrectomy. The diagnosis was mucinous cystadenoma of the kidney. No recurrence was observed 6 months after surgery. The histopathology was unique since the inner surface of the cyst was covered by a mucin-positive columnar epithelium connected to a urothelium, with continuous transition between the two...
January 2015: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/25592431/type-2-diabetes-reduces-the-proliferation-and-survival-of-oligodendrocyte-progenitor-cells-in-ishchemic-white-matter-lesions
#18
Y Yatomi, R Tanaka, Y Shimada, K Yamashiro, M Liu, Y Mitome-Mishima, N Miyamoto, Y Ueno, T Urabe, N Hattori
Diabetes mellitus (DM) is a major risk factor for stroke and it exacerbates tissue damage after ischemic insult. Diabetes is one of the important causes of the progression of white matter lesion, however, the pathological mechanisms remain unclear. The present study evaluated the influences of type 2 DM on ischemic subcortical white matter injury and the recruitment of oligodendrocyte progenitor cells (OPCs) under chronic cerebral hypoperfusion using type 2 diabetic (db/db) mice. After bilateral common carotid artery stenosis (BCAS), the rarefaction in the white matter was more severe in db/db mice than in db/+ mice, and the number of glutathione S-transferase-pi (GST-pi)-positive mature oligodendrocytes (OLG) was lower in db/db mice than in db/+ mice at 4 and 8 weeks after ischemia...
March 19, 2015: Neuroscience
https://www.readbyqxmd.com/read/25573082/controlling-the-redox-properties-of-a-pyrroloquinolinequinone-pqq-derivative-in-a-ruthenium-ii-coordination-sphere
#19
Hiroumi Mitome, Tomoya Ishizuka, Yoshihito Shiota, Kazunari Yoshizawa, Takahiko Kojima
Ruthenium(ii) complexes of PQQTME, a trimethyl ester derivative of redox-active PQQ (pyrroloquinolinequinone), were prepared using a tridentate ligand, 2,2':6',2''-terpyridine (terpy) as an auxiliary ligand. The characterization of the complexes was performed by spectroscopic methods, X-ray crystallography, and electrochemical measurements. In one complex, the pyridine site of PQQTME binds to the [Ru(II)(terpy)] unit as a tridentate ligand, and a silver(i) ion is coordinated by the quinone moiety in a bidentate fashion...
February 21, 2015: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/25259873/magnetoresistance-of-drop-cast-film-of-cobalt-substituted-magnetite-nanocrystals
#20
Shigemi Kohiki, Koichiro Nara, Masanori Mitome, Daiju Tsuya
An oleic acid-coated Fe2.7Co0.3O4 nanocrystal (NC) self-assembled film was fabricated via drop casting of colloidal particles onto a three-terminal electrode/MgO substrate. The film exhibited a large coercivity (1620 Oe) and bifurcation of the zero-field-cooled and field-cooled magnetizations at 300 K. At 10 K, the film exhibited both a Coulomb blockade due to single electron charging as well as a magnetoresistance of ∼-80% due to spin-dependent electron tunneling. At 300 K, the film also showed a magnetoresistance of ∼-80% due to hopping of spin-polarized electrons...
October 22, 2014: ACS Applied Materials & Interfaces
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