keyword
MENU ▼
Read by QxMD icon Read
search

single particle tracking

keyword
https://www.readbyqxmd.com/read/28094297/high-throughput-quantitation-of-inorganic-nanoparticle-biodistribution-at-the-single-cell-level-using-mass-cytometry
#1
Yu-Sang Sabrina Yang, Prabhani U Atukorale, Kelly D Moynihan, Ahmet Bekdemir, Kavya Rakhra, Li Tang, Francesco Stellacci, Darrell J Irvine
Inorganic nanoparticles (NPs) are studied as drug carriers, radiosensitizers and imaging agents, and characterizing nanoparticle biodistribution is essential for evaluating their efficacy and safety. Tracking NPs at the single-cell level with current technologies is complicated by the lack of reliable methods to stably label particles over extended durations in vivo. Here we demonstrate that mass cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of ∼10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping...
January 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28088300/cytoplasmic-rna-protein-particles-exhibit-non-gaussian-subdiffusive-behavior
#2
Thomas J Lampo, Stella Stylianidou, Mikael P Backlund, Paul A Wiggins, Andrew J Spakowitz
The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia coli and Saccharomyces cerevisiae cells. As shown previously, these RNA-protein particles exhibit subdiffusive behavior that is viscoelastic in its origin. Examining the ensemble of particle displacements reveals a Laplace distribution at all observed timescales rather than the Gaussian distribution predicted by the central limit theorem...
January 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28081290/membrane-microdomains-and-the-cytoskeleton-constrain-athir1-dynamics-and-facilitate-the-formation-of-an-athir1-associated-immune-complex
#3
Xueqin Lv, Yanping Jing, Jianwei Xiao, Yongdeng Zhang, Yingfang Zhu, Russell Julian, Jinxing Lin
Arabidopsis hypersensitive induced reaction (AtHIR) proteins function in plant innate immunity. However, the underlying mechanisms by which AtHIRs participate in plant immunity remain elusive. Here, using VA-TIRFM and FLIM-FRET, we revealed that AtHIR1 is present in membrane microdomains and co-localizes with the membrane microdomain marker REM1.3. Single-particle tracking analysis revealed that membrane microdomains and the cytoskeleton, especially microtubules, restrict the lateral mobility of AtHIR1 at the plasma membrane and facilitate its oligomerization...
January 12, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28079956/an-exonuclease-iii-powered-on-particle-stochastic-dna-walker
#4
Xiangmeng Qu, Dan Zhu, Guangbao Yao, Shao Su, Jie Chao, Huajie Liu, Xiaolei Zuo, Lihua Wang, Jiye Shi, Lianhui Wang, Wei Huang, Hao Pei, Chunhai Fan
DNA-based machines have attracted rapidly growing interest owing to their potential in drug delivery, biocomputing, and diagnostic applications. Herein, we report a type of exonuclease III (Exo III)-powered stochastic DNA walker that can autonomously move on a spherical nucleic acid (SNA)-based 3D track. The motion is propelled by unidirectional Exo III digestion of hybridized DNA tracks in a burnt-bridge mechanism. The operation of this Exo III-propelled DNA walker was monitored in real time and at the single-particle resolution using total internal reflection fluorescence microscopy (TIRF)...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28076813/signatures-of-mechanosensitive-gating
#5
REVIEW
Richard G Morris
The question of how mechanically gated membrane channels open and close is notoriously difficult to address, especially if the protein structure is not available. This perspective highlights the relevance of micropipette-aspirated single-particle tracking-used to obtain a channel's diffusion coefficient, D, as a function of applied membrane tension, σ-as an indirect assay for determining functional behavior in mechanosensitive channels. While ensuring that the protein remains integral to the membrane, such methods can be used to identify not only the gating mechanism of a protein, but also associated physical moduli, such as torsional and dilational rigidity, which correspond to the protein's effective shape change...
January 10, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28071667/photogate-microscopy-to-track-single-molecules-in-crowded-environments
#6
Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E Lawrence, Roberto Zoncu, Ahmet Yildiz
Tracking single molecules inside cells reveals the dynamics of biological processes, including receptor trafficking, signalling and cargo transport. However, individual molecules often cannot be resolved inside cells due to their high density. Here we develop the PhotoGate technique that controls the number of fluorescent particles in a region of interest by repeatedly photobleaching its boundary. PhotoGate bypasses the requirement of photoactivation to track single particles at surface densities two orders of magnitude greater than the single-molecule detection limit...
January 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28068671/high-spatial-resolution-mapping-of-catalytic-reactions-on-single-particles
#7
Chung-Yeh Wu, William J Wolf, Yehonatan Levartovsky, Hans A Bechtel, Michael C Martin, F Dean Toste, Elad Gross
The critical role in surface reactions and heterogeneous catalysis of metal atoms with low coordination numbers, such as found at atomic steps and surface defects, is firmly established. But despite the growing availability of tools that enable detailed in situ characterization, so far it has not been possible to document this role directly. Surface properties can be mapped with high spatial resolution, and catalytic conversion can be tracked with a clear chemical signature; however, the combination of the two, which would enable high-spatial-resolution detection of reactions on catalytic surfaces, has rarely been achieved...
January 11, 2017: Nature
https://www.readbyqxmd.com/read/28067508/coherent-brightfield-microscopy-provides-the-spatiotemporal-resolution-to-study-early-stage-viral-infection-in-live-cells
#8
Yi-Fan Huang, Guan-Yu Zhuo, Chun-Yu Chou, Cheng-Hao Lin, Wen Chang, Chia-Lung Hsieh
Viral infection starts with a virus particle landing on a cell surface followed by penetration of the plasma membrane. Due to the difficulty of measuring the rapid motion of small-sized virus particles on the membrane, little is known about how a virus particle reaches an endocytic site after landing at a random location. Here, we use coherent brightfield (COBRI) microscopy to investigate early stage viral infection with ultrahigh spatiotemporal resolution. By detecting intrinsic scattered light via imaging-based interferometry, COBRI microscopy allows us to track the motion of a single vaccinia virus particle with nanometer spatial precision (<3 nm) in 3D and microsecond temporal resolution (up to 100,000 frames per second)...
January 13, 2017: ACS Nano
https://www.readbyqxmd.com/read/28046397/we-h-bra-07-mechanistic-modelling-of-the-relative-biological-effectiveness-of-heavy-charged-particles
#9
S McMahon, A McNamara, J Schuemann, K Prise, H Paganetti
Purpose Uncertainty in the Relative Biological Effectiveness (RBE) of heavy charged particles compared to photons remains one of the major uncertainties in particle therapy. As RBEs depend strongly on clinical variables such as tissue type, dose, and radiation quality, more accurate individualised models are needed to fully optimise treatments. MethodsWe have developed a model of DNA damage and repair following X-ray irradiation in a number of settings, incorporating mechanistic descriptions of DNA repair pathways, geometric effects on DNA repair, cell cycle effects and cell death...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28045263/single-and-double-strand-breaks-of-dry-dna-exposed-to-protons-at-bragg-peak-energies
#10
Mounir Souici, Talat T Khalil, Dominique Muller, Quentin Raffy, Rémi Barillon, Abdelfettah Belafrites, Christophe Champion, Michel Fromm
Ultrathin layers (<20 nm) of pBR322 plasmid DNA were deposited onto 2.5 μm thick polyester films and exposed to proton Bragg-peak energies (90-3000 keV) at various fluences. A quantitative analysis of radio-induced DNA damage is reported here in terms of single- and double-strand breaks (SSB and DSB, respectively). The corresponding yields as well as G-values and the cross sections exhibit fairly good agreement with the rare available data, stemming from close experimental conditions, namely, based on α particle irradiation...
January 17, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28045048/in-vivo-single-molecule-imaging-of-syntaxin1a-reveals-polyphosphoinositide-and-activity-dependent-trapping-in-presynaptic-nanoclusters
#11
Adekunle T Bademosi, Elsa Lauwers, Pranesh Padmanabhan, Lorenzo Odierna, Ye Jin Chai, Andreas Papadopulos, Geoffrey J Goodhill, Patrik Verstreken, Bruno van Swinderen, Frédéric A Meunier
Syntaxin1A is organized in nanoclusters that are critical for the docking and priming of secretory vesicles from neurosecretory cells. Whether and how these nanoclusters are affected by neurotransmitter release in nerve terminals from a living organism is unknown. Here we imaged photoconvertible syntaxin1A-mEos2 in the motor nerve terminal of Drosophila larvae by single-particle tracking photoactivation localization microscopy. Opto- and thermo-genetic neuronal stimulation increased syntaxin1A-mEos2 mobility, and reduced the size and molecular density of nanoclusters, suggesting an activity-dependent release of syntaxin1A from the confinement of nanoclusters...
January 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/28035048/live-cell-single-molecule-labeling-and-analysis-of-myosin-motors-with-quantum-dots
#12
Hiroyasu Hatakeyama, Yoshihito Nakahata, Hirokazu Yarimizu, Makoto Kanzaki
Quantum dots (QDs) are a powerful tool for quantitatively analyzing dynamic cellular processes by single-particle tracking. However, tracking of intracellular molecules with QDs is limited by their inability to penetrate the plasma membrane and bind to specific molecules of interest. Although several techniques for overcoming these problems have been proposed, they are either complicated or inconvenient. To address this issue, in this study, we developed a simple, convenient, and nontoxic method for labeling intracellular molecules in cells using HaloTag technology and electroporation...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28033200/lvad-outflow-graft-angle-and-thrombosis-risk
#13
Alberto Aliseda, Venkat Keshav Chivukula, Patrick Mcgah, Anthony R Prisco, Jennifer A Beckman, Guilherme J M Garcia, Nahush A Mokadam, Claudius Mahr
This study quantifies thrombogenic potential (TP) of a wide range of left ventricular assist device (LVAD) outflow graft anastomosis angles through state-of-the-art techniques: 3D imaged-based patient-specific models created via virtual surgery and unsteady computational fluid dynamics with Lagrangian particle tracking. This study aims at clarifying the influence of a single parameter (outflow graft angle) on the thrombogenesis associated with flow patterns in the aortic root after LVAD implantation. This is an important and poorly-understood aspect of LVAD therapy, because several studies have shown strong inter and intrapatient thrombogenic variability and current LVAD implantation strategies do not incorporate outflow graft angle optimization...
January 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/28033028/single-particle-analysis-methods-for-detection-of-platelet-extracellular-vesicles-in-suspension-excluding-flow-cytometry
#14
Edit I Buzás, Chris Gardiner, Changwon Lee, Zachary J Smith
Extracellular vesicles (EVs) are small, membrane-bound particles released by all cell types, including abundant release by platelets. EVs are a topic of increasing interest in the academic and clinical community due to their increasingly recognised and diverse role in normal biology as well as in disease. However, typical analysis methods to characterise EVs released by cultured cells or isolated from whole blood or other body fluids are restricted to bulk analysis of all EVs in a sample. In this review, we discuss the motivation for analysis of individual EVs, as well as discuss three emerging methods for physical and chemical characterisation of individual EVs: nanoparticle tracking analysis, tunable resistive pulse sensing and Raman spectroscopy...
December 29, 2016: Platelets
https://www.readbyqxmd.com/read/28032533/tetraspanin-tspan9-regulates-platelet-collagen-receptor-gpvi-lateral-diffusion-and-activation
#15
Elizabeth J Haining, Alexandra L Matthews, Peter J Noy, Hanna M Romanska, Helen J Harris, Jeremy Pike, Martina Morowski, Rebecca L Gavin, Jing Yang, Pierre-Emmanuel Milhiet, Fedor Berditchevski, Bernhard Nieswandt, Natalie S Poulter, Steve P Watson, Michael G Tomlinson
The tetraspanins are a superfamily of four-transmembrane proteins, which regulate the trafficking, lateral diffusion and clustering of the transmembrane proteins with which they interact. We have previously shown that tetraspanin Tspan9 is expressed on platelets. Here we have characterised gene-trap mice lacking Tspan9. The mice were viable with normal platelet numbers and size. Tspan9-deficient platelets were specifically defective in aggregation and secretion induced by the platelet collagen receptor GPVI, despite normal surface GPVI expression levels...
December 29, 2016: Platelets
https://www.readbyqxmd.com/read/28029254/pegylated-and-functionalized-aliphatic-polycarbonate-polyplex-nanoparticles-for-intravenous-administration-of-hdac5-sirna-in-cancer-therapy
#16
Antoine Frère, Alexandra Baroni, Elodie Hendrick, Anne-Sophie Delvigne, François Orange, Olivier Peulen, George R Dakwar, Jérôme Diricq, Philippe Dubois, Brigitte Evrard, Katrien Remaut, Kevin Braeckmans, Stefaan C De Smedt, Julie Laloy, Jean-Michel Dogné, Georges Feller, Laetitia Mespouille, Denis Mottet, Geraldine Piel
Guanidine and morpholine functionalized aliphatic polycarbonate polymers able to efficiently deliver histone deacetylase 5 (HDAC5) siRNA into the cytoplasm of cancer cells in vitro leading to a decrease of cell proliferation were previously developed. To allow these biodegradable and biocompatible polyplex nanoparticles to overcome the extracellular barriers and be effective in vivo after an intravenous injection, polyethylene glycol chains (PEG750 or PEG2000) were grafted on the polymer structure. These nanoparticles, showed an average size of about 150 nm and a slightly positive zeta potential with complete siRNA complexation...
December 28, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28028226/single-pixel-interior-filling-function-approach-for-detecting-and-correcting-errors-in-particle-tracking
#17
Stanislav Burov, Patrick Figliozzi, Binhua Lin, Stuart A Rice, Norbert F Scherer, Aaron R Dinner
We present a general method for detecting and correcting biases in the outputs of particle-tracking experiments. Our approach is based on the histogram of estimated positions within pixels, which we term the single-pixel interior filling function (SPIFF). We use the deviation of the SPIFF from a uniform distribution to test the veracity of tracking analyses from different algorithms. Unbiased SPIFFs correspond to uniform pixel filling, whereas biased ones exhibit pixel locking, in which the estimated particle positions concentrate toward the centers of pixels...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28017738/validation-of-the-radiobiology-toolkit-topas-nbio-in-simple-dna-geometries
#18
Aimee McNamara, Changran Geng, Robert Turner, Jose Ramos Mendez, Joseph Perl, Kathryn Held, Bruce Faddegon, Harald Paganetti, Jan Schuemann
Computational simulations offer a powerful tool for quantitatively investigating radiation interactions with biological tissue and can help bridge the gap between physics, chemistry and biology. The TOPAS collaboration is tackling this challenge by extending the current Monte Carlo tool to allow for sub-cellular in silico simulations in a new extension, TOPAS-nBio. TOPAS wraps and extends the Geant4 Monte Carlo simulation toolkit and the new extension allows the modeling of particles down to vibrational energies (∼2eV) within realistic biological geometries...
December 22, 2016: Physica Medica: PM
https://www.readbyqxmd.com/read/28002733/single-molecule-dna-polymerase-dynamics-at-a-bacterial-replisome-in-live-cells
#19
Yi Liao, Yilai Li, Jeremy W Schroeder, Lyle A Simmons, Julie S Biteen
PolC is one of two essential replicative DNA polymerases found in the Gram-positive bacterium Bacillus subtilis. The B. subtilis replisome is eukaryotic-like in that it relies on a two DNA polymerase system for chromosomal replication. To quantitatively image how the replicative DNA polymerase PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional superresolution imaging, and single-particle tracking to examine the in vivo behavior of PolC at single-molecule resolution...
December 20, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27995796/tracking-nanoparticle-diffusion-and-interaction-during-self-assembly-in-a-liquid-cell
#20
Alexander S Powers, Hong-Gang Liao, Shilpa N Raja, Noah D Bronstein, A Paul Alivisatos, Haimei Zheng
Nanoparticle self-assembly has been well studied theoretically, but it remains challenging to directly observe and quantify individual nanoparticle interactions. With our custom image analysis method, we track the trajectories of nanoparticle movement with high precision from a stack of relatively noisy images obtained using liquid cell transmission electron microscopy. In a time frame of minutes, Pt-Fe nanoparticles self-assembled into a loosely packed hcp lattice. The energetics and stability of the dynamic assembly were studied quantitatively...
December 20, 2016: Nano Letters
keyword
keyword
33260
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"