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https://www.readbyqxmd.com/read/29780374/insight-into-the-variation-of-bacterial-structure-in-atrazine-contaminated-soil-regulating-by-potential-phytoremediator-pennisetum-americanum-l-k-schum
#1
Bo Cao, Ying Zhang, Ziyi Wang, Mengyuan Li, Feng Yang, Duo Jiang, Zhao Jiang
Although plants of the genus Pennisetum can accelerate the removal of atrazine from its rhizosphere, the roles played by this plant in adjusting the soil environment and soil microorganism properties that might contribute to pollutant removal are incompletely understood. We selected Pennisetum americanum (L.) K. Schum ( P. americanum ) as the test plant and investigated the interaction between P. americanum and atrazine-contaminated soil, focusing on the adjustment of the soil biochemical properties as well as bacterial functional and community diversity in the rhizosphere using Biolog EcoPlates and high-throughput sequencing of the 16S rRNA gene...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29710570/microplastics-new-substrates-for-heterotrophic-activity-contribute-to-altering-organic-matter-cycles-in-aquatic-ecosystems
#2
Maria Arias-Andres, Marie Therese Kettner, Takeshi Miki, Hans-Peter Grossart
Heterotrophic microbes with the capability to process considerable amounts of organic matter can colonize microplastic particles (MP) in aquatic ecosystems. Weather colonization of microorganisms on MP will alter ecological niche and functioning of microbial communities remains still unanswered. Therefore, we compared the functional diversity of biofilms on microplastics when incubated in three lakes in northeastern Germany differing in trophy and limnological features. For all lakes, we compared heterotrophic activities of MP biofilms with those of microorganisms in the surrounding water by using Biolog® EcoPlates and assessed their oxygen consumption in microcosm assays with and without MP...
April 21, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29551210/microbial-communities-and-their-potential-for-degradation-of-dissolved-organic-carbon-in-cryoconite-hole-environments-of-himalaya-and-antarctica
#3
Aritri Sanyal, Runa Antony, Gautami Samui, Meloth Thamban
Cryoconite holes (cylindrical melt-holes on the glacier surface) are important hydrological and biological systems within glacial environments that support diverse microbial communities and biogeochemical processes. This study describes retrievable heterotrophic microbes in cryoconite hole water from three geographically distinct sites in Antarctica, and a Himalayan glacier, along with their potential to degrade organic compounds found in these environments. Microcosm experiments (22 days) show that 13-60% of the dissolved organic carbon in the water within cryoconite holes is bio-available to resident microbes...
March 2018: Microbiological Research
https://www.readbyqxmd.com/read/29529564/pretreatment-of-saline-antibiotic-wastewater-using-marine-microalga
#4
Xueqing Shi, Tsuey Shan Yeap, Shujuan Huang, Jianqiu Chen, How Yong Ng
A green microalga Chlorella sp. isolated from marine environment was investigated for its potential to pretreat saline antibiotic wastewater containing amoxicillin (AMX). Through Biolog EcoPlate assay, the Chlorella sp. showed its unique carbon source metabolic patterns under autotrophic condition. In addition, the microalga could effectively remove AMX (>99%) under initial AMX concentrations ranging from 10 to 150 mg/L through a treatability test. In the continuous AMX treatment using a lab-scale membrane photobioreactor (MPBR), a stable AMX removal efficiency of 85...
June 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29454207/repeated-treatments-of-ciprofloxacin-and-kresoxim-methyl-alter-their-dissipation-rates-biological-function-and-increase-antibiotic-resistance-in-manured-soil
#5
Hua Fang, Lingxi Han, Houpu Zhang, Yanfei Deng, Qiqing Ge, Jiajia Mei, Zhengnan Long, Yunlong Yu
The dissipation of ciprofloxacin (CIP, 1.0 and 10.0mg/kg) and kresoxim-methyl (KM, 1.0 and 2.0mg/kg) in manure-amended soil, the variations in soil enzyme activities and microbial functional diversities, and CIP-induced bacterial community tolerances were studied using a chromatographic analysis, enzyme colorimetric and titration analyses, and the BIOLOG EcoPlate method. Three successive treatments of individual and combined samples of CIP and KM at low and high concentrations were performed at 60d intervals...
July 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29453267/plant-microbe-and-abiotic-factors-influencing-salmonella-survival-and-growth-on-alfalfa-sprouts-and-swiss-chard-microgreens
#6
Elizabeth Reed, Christina M Ferreira, Rebecca Bell, Eric W Brown, Jie Zheng
Microgreens, like sprouts, are relatively fast-growing products and are generally consumed raw. Moreover, as observed for sprouts, microbial contamination from preharvest sources may also be present in the production of microgreens. In this study, two Salmonella enterica serovars (Hartford and Cubana), applied at multiple inoculation levels, were evaluated for survival and growth on alfalfa sprouts and Swiss chard microgreens by using the most-probable-number (MPN) method. Various abiotic factors were also examined for their effects on Salmonella survival and growth on sprouts and microgreens...
May 1, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29382933/characterization-of-soil-bacterial-archaeal-and-fungal-communities-inhabiting-archaeological-human-impacted-layers-at-monte-iato-settlement-sicily-italy
#7
José A Siles, Birgit Öhlinger, Tomas Cajthaml, Erich Kistler, Rosa Margesin
Microbial communities in human-impacted soils of ancient settlements have been proposed to be used as ecofacts (bioindicators) of different ancient anthropogenic activities. In this study, bacterial, archaeal and fungal communities inhabiting soil of three archaic layers, excavated at the archaeological site on Monte Iato (Sicily, Italy) and believed to have been created in a chronological order in archaic times in the context of periodic cultic feasts, were investigated in terms of (i) abundance (phospholipid fatty acid (PLFA) analysis and quantitative PCR)), (ii) carbon(C)-source consumption patterns (Biolog-Ecoplates) and (iii) diversity and community composition (Illumina amplicon sequencing)...
January 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29335587/bacterial-and-fungal-community-composition-and-functional-activity-associated-with-lake-wetland-water-level-gradients
#8
Yantian Ma, Jinqian Li, Juan Wu, Zhaoyu Kong, Larry M Feinstein, Xia Ding, Gang Ge, Lan Wu
The water regime is often the primary force driving the evolution of freshwater lakes, but how soil microbes responded to this process is far from understood. This study sampled wetland soils from a shallow lake that experienced water regime changes, Poyang Lake of China, to explore the features of bacterial and fungal community in response to water level changes. The soil physicochemical properties, T-RFLP based community structures and soil activities (including basal respiration, microbial biomass and enzymes) were all determined...
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29056380/association-between-degradation-of-pharmaceuticals-and-endocrine-disrupting-compounds-and-microbial-communities-along-a-treated-wastewater-effluent-gradient-in-lake-mead
#9
Susanna M Blunt, Joshua D Sackett, Michael R Rosen, Mark J Benotti, Rebecca A Trenholm, Brett J Vanderford, Brian P Hedlund, Duane P Moser
The role of microbial communities in the degradation of trace organic contaminants in the environment is little understood. In this study, the biotransformation potential of 27 pharmaceuticals and endocrine-disrupting compounds was examined in parallel with a characterization of the native microbial community in water samples from four sites variously impacted by urban run-off and wastewater discharge in Lake Mead, Nevada and Arizona, USA. Samples included relatively pristine Colorado River water at the upper end of the lake, nearly pure tertiary-treated municipal wastewater entering via the Las Vegas Wash, and waters of mixed influence (Las Vegas Bay and Boulder Basin), which represented a gradient of treated wastewater effluent impact...
May 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28688360/microbial-functional-diversity-responses-to-2-years-since-biochar-application-in-silt-loam-soils-on-the-loess-plateau
#10
Li-Xia Zhu, Qian Xiao, Yu-Fang Shen, Shi-Qing Li
The structure and function of soil microbial communities have been widely used as indicators of soil quality and fertility. The effect of biochar application on carbon sequestration has been studied, but the effect on soil microbial functional diversity has received little attention. We evaluated effects of biochar application on the functional diversities of microbes in a loam soil. The effects of biochar on microbial activities and related processes in the 0-10 and 10-20cm soil layers were determined in a two-year experiment in maize field on the Loess Plateau in China...
October 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28585175/nematicidal-effect-of-rhizobacteria-on-plant-parasitic-nematodes-associated-with-vineyards
#11
E Aballay, S Prodan, A Zamorano, C Castaneda-Alvarez
The action of metabolites and exoenzymes from rhizobacteria on different plant-parasitic nematodes has an influence on the nematicidal efficacy of the microbe. Seven rhizobacteria, divided into two bacterial groups, were evaluated in vitro for nematicidal activity on Meloidogyne ethiopica and Xiphinema index. The direct effect of their filtrates on egg hatching and juveniles of M. ethiopica as well as mobile stages of X. index was evaluated during a 72-h period. The production of four exoenzymes and two metabolites associated with nematode mortality was investigated...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28581020/insights-into-the-fecal-microbiota-of-captive-iberian-lynx-lynx-pardinus
#12
José Alberto Núñez-Díaz, M Carmen Balebona, Eva M Alcaide, Irene Zorrilla, Miguel Ángel Moriñigo
The Iberian lynx (Lynx pardinus) is an endangered species restricted to several areas of Spain and Portugal. Its low genetic diversity likely provokes immune depression and high susceptibility to infectious diseases. The intestinal microbiota is closely related to host health and nutrition. In order to contribute to the knowledge of the Iberian lynx intestinal microbiota, fecal microbiota of captive specimens from two breeding centers ("La Olivilla" and "El Acebuche"), located in Southern Spain, were studied by Denaturing Gradient Gel Electrophoresis (DGGE)...
March 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28432990/influence-of-red-mud-on-soil-microbial-communities-application-and-comprehensive-evaluation-of-the-biolog-ecoplate-approach-as-a-tool-in-soil-microbiological-studies
#13
Viktória Feigl, Éva Ujaczki, Emese Vaszita, Mónika Molnár
Red mud can be applied as soil ameliorant to acidic, sandy and micronutrient deficient soils. There are still knowledge gaps regarding the effects of red mud on the soil microbial community. The Biolog EcoPlate technique is a promising tool for community level physiological profiling. This study presents a detailed evaluation of Biolog EcoPlate data from two case studies. In experiment "A" red mud from Ajka (Hungary) was mixed into acidic sandy soil in soil microcosms at 5-50 w/w%. In experiement "B" red mud soil mixture was mixed into low quality subsoil in a field experiment at 5-50 w/w%...
October 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28429833/the-microbially-mediated-soil-organic-carbon-loss-under-degenerative-succession-in-an-alpine-meadow
#14
Yuguang Zhang, Xiao Liu, Jing Cong, Hui Lu, Yuyu Sheng, Xiulei Wang, Diqiang Li, Xueduan Liu, Huaqun Yin, Jizhong Zhou, Ye Deng
Land-cover change has long been recognized as having marked effect on the amount of soil organic carbon (SOC). However, the microbially mediated processes and mechanisms on SOC are still unclear. In this study, the soil samples in a degenerative succession from alpine meadow to alpine steppe meadow in the Qinghai-Tibetan Plateau were analysed using high-throughput technologies, including Illumina sequencing and geochip functional gene arrays. The soil microbial community structure and diversity were significantly (p < ...
July 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28395259/effects-of-18-pharmaceuticals-on-the-physiological-diversity-of-edaphic-microorganisms
#15
Mª Rosa Pino-Otín, Selene Muñiz, Jonatan Val, Enrique Navarro
Pharmaceutical residues can enter the terrestrial environment through the application of recycled water and contaminated biosolids to agricultural soils, were edaphic microfauna can would be threatened. This study thus assessed the effect of 18 widely consumed pharmaceuticals, belonging to four groups: antibiotics, non-steroidal anti-inflammatory drugs (NSAIDs), blood lipid-lowering agents (BLLA) and β-blockers, on the physiology of soil microbial communities from a ecological crop field. Biolog EcoPlates, containing 31 of the most common carbon sources found in forest and crop soils, were used to calculate both the averaged well colour development (AWCD), as an indicator of the entire capacity of degrading carbon sources, and the diversity of carbon source utilization, as an indicator of the physiological diversity...
October 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28300176/nanoscale-zero-valent-iron-persulfate-enhanced-upflow-anaerobic-sludge-blanket-reactor-for-dye-removal-insight-into-microbial-metabolism-and-microbial-community
#16
Fei Pan, Xiaohan Zhong, Dongsheng Xia, Xianze Yin, Fan Li, Dongye Zhao, Haodong Ji, Wen Liu
This study investigated the efficiency of nanoscale zero-valent iron combined with persulfate (NZVI/PS) for enhanced degradation of brilliant red X-3B in an upflow anaerobic sludge blanket (UASB) reactor, and examined the effects of NZVI/PS on anaerobic microbial communities during the treatment process. The addition of NZVI (0.5 g/L) greatly enhanced the decolourization rate of X-3B from 63.8% to 98.4%. The Biolog EcoPlate(TM) technique was utilized to examine microbial metabolism in the reactor, and the Illumina MiSeq high-throughput sequencing revealed 22 phyla and 88 genera of the bacteria...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28289836/prokaryotic-abundance-and-activity-in-permafrost-of-the-northern-victoria-land-and-upper-victoria-valley-antarctica
#17
Rosabruna La Ferla, Maurizio Azzaro, Luigi Michaud, Gabriella Caruso, Angelina Lo Giudice, Rodolfo Paranhos, Anderson S Cabral, Antonella Conte, Alessandro Cosenza, Giovanna Maimone, Maria Papale, Alessandro Ciro Rappazzo, Mauro Guglielmin
Victoria Land permafrost harbours a potentially large pool of cold-affected microorganisms whose metabolic potential still remains underestimated. Three cores (BC-1, BC-2 and BC-3) drilled at different depths in Boulder Clay (Northern Victoria Land) and one sample (DY) collected from a core in the Dry Valleys (Upper Victoria Valley) were analysed to assess the prokaryotic abundance, viability, physiological profiles and potential metabolic rates. The cores drilled at Boulder Clay were a template of different ecological conditions (different temperature regime, ice content, exchanges with atmosphere and with liquid water) in the same small basin while the Dry Valleys site was very similar to BC-2 conditions but with a complete different geological history and ground ice type...
August 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28054272/enhanced-bacterial-affinity-of-pvdf-membrane-its-application-as-improved-sea-water-sampling-tool-for-environmental-monitoring
#18
Sweta Binod Kumar, Preeti Sharnagat, Paramita Manna, Amit Bhattacharya, Soumya Haldar
Isolation of diversified bacteria from seawater is a major challenge in the field of environmental microbiology. In the present study, an attempt has been made to select specific membrane with improved property of attaching diversified bacteria. Initially, different concentrations (15, 18, and 20% W/W) of polysulfone (PSF) were used to check their affinity for the attachment of selected gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. Among these, 20% W/W PSF showed maximum attachment...
February 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28011364/functionality-of-microbial-communities-in-constructed-wetlands-used-for-pesticide-remediation-influence-of-system-design-and-sampling-strategy
#19
Tao Lv, Pedro N Carvalho, Liang Zhang, Yang Zhang, Mark Button, Carlos A Arias, Kela P Weber, Hans Brix
The objective of this study was to compare the microbial community metabolic function from both unsaturated and saturated constructed wetland mesocosms (CWs) when treating the pesticide tebuconazole. The comparison was performed for both interstitial water and substrate biofilm by community level physiological profiling (CLPP) via BIOLOG™ EcoPlates. For each CW design (saturated or unsaturated), six mesocosms were established including one unplanted and five planted individually with either Juncus effusus, Typha latifolia, Berula erecta, Phragmites australis or Iris pseudacorus...
March 1, 2017: Water Research
https://www.readbyqxmd.com/read/27807655/profile-of-citrobacter-freundii-st2-a-multi-acyl-homoserine-lactone-producer-associated-with-marine-dinoflagellates
#20
Xinqi Huang, Yan Gao, Zhiping Ma, Guanghui Lin, Zhonghua Cai, Jin Zhou
Marine algae provide a unique niche termed the phycosphere for microorganism inhabitation. The phycosphere environment is an important niche for mutualistic and competitive interactions between algae and bacteria. Quorum sensing (QS) serves as a gene regulatory system in the microbial biosphere that allows bacteria to sense the population density with signaling molecules, such as acyl-homoserine lactone (AHL), and adapt their physiological activities to their surroundings. Understanding the QS system is important to elucidate the interactions between algal-associated microbial communities in the phycosphere condition...
January 2017: Current Microbiology
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