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ecoplates biolog

Marek Pająk, Ewa Błońska, Magdalena Frąc, Karolina Oszust
The objective of this study was to assess the impact of metal contamination on microbial functional diversity and enzyme activity in forest soils. This study involved the evaluation of the influence of the texture, carbon content and distance to the source of contamination on the change in soil microbial activity, which did not investigate in previous studies. The study area is located in southern Poland near the city of Olkusz around the flotation sedimentation pond of lead and zinc at the Mining and Metallurgical Company "ZGH Bolesław, Inc...
2016: Water, Air, and Soil Pollution
Clemencia Chaves Lopez, Annalisa Serio, Chiara Rossi, Giovanni Mazzarrino, Sonia Marchetti, Federica Castellani, Lisa Grotta, Francesco Paolo Fiorentino, Antonello Paparella, Giuseppe Martino
Iodine deficiency remains a major public health concern in many countries, including some European regions. This study aimed at understanding the effect of a supplement of marine alga Ascophyllum nodosum as a iodine fortifier in the cow diet, on the compositional and microbiological quality of milk. The results obtained in this work indicated that the dietary inclusion of A. nodosum exerted significant effects on cow milk composition. In particular, it increased iodine content and reduced the quantity of free amino acids without modifying the free fatty acid content...
August 2016: Journal of Dairy Science
Lin Fu, Yunze Ruan, Chengyuan Tao, Rong Li, Qirong Shen
Fusarium wilt of banana always drives farmers to find new land for banana cultivation due to the comeback of the disease after a few cropping years. A novel idea for solving this problem is the continuous application of bioorganic fertilizer (BIO), which should be practiced from the beginning of banana planting. In this study, BIO was applied in newly reclaimed fields to pre-control banana Fusarium wilt and the culturable rhizobacteria community were evaluated using Biolog Ecoplates and culture-dependent denaturing gradient gel electrophoresis (CD-DGGE)...
2016: Scientific Reports
Anna Siczek, Jerzy Lipiec
Inoculation of legume seeds with Rhizobium affects soil microbial community and processes, especially in the rhizosphere. This study aimed at assessing the effect of Rhizobium inoculation on microbial activity in the faba bean rhizosphere during the growing season in a field experiment on a Haplic Luvisol derived from loess. Faba bean (Vicia faba L.) seeds were non-inoculated (NI) or inoculated (I) with Rhizobium leguminosarum bv. viciae and sown. The rhizosphere soil was analyzed for the enzymatic activities of dehydrogenases, urease, protease and acid phosphomonoesterase, and functional diversity (catabolic potential) using the Average Well Color Development, Shannon-Weaver, and Richness indices following the community level physiological profiling from Biolog EcoPlate™...
2016: International Journal of Molecular Sciences
Christin Säwström, Oscar Serrano, Mohammad Rozaimi, Paul S Lavery
Coastal vegetated ecosystems play an important role in carbon cycling and bacterial communities inhabiting coastal sediments are responsible for the remineralization and processing of organic carbon (OC). We collected 1 m-long sediment cores in Posidonia seagrass meadows from coastal and estuarine sites in Australia that differed in their sedimentary organic and inorganic carbon, nitrogen and mud contents. The metabolic diversity of sediment heterotrophic bacterial communities was characterized at different sediment depths, based on the utilization pattern of 31 individual carbon substrates using Biolog EcoPlates(TM) ...
May 18, 2016: Environmental Microbiology Reports
Feng-wu Wang, Wei Liu, Juan-juan Wan, Jia-li Yang, Xue-mei Liu, Su-lin Xiang, Yong-hong Wu
So far, many types of carriers (such as artificial mat, industrial soft carriers) have been widely used in removing pollutants, purifying water quality via the periphyton attached on the surface of these carriers. In the presence of periphyton, the distribution of redox zone at different water layers is directly or indirectly associated with the removal rate of pollutants. Therefore, it is more practically significant to study the distribution of redox zone at different water layers and the microbial diversity in the presence of periphyton...
November 2015: Huan Jing Ke Xue= Huanjing Kexue
Joanna Chojniak, Łukasz Jałowiecki, Elmar Dorgeloh, Berta Hegedusova, Helene Ejhed, Jörgen Magnér, Grażyna Płaza
The scope of this study was to apply the Biolog system to identify and characterize a Serratia strain isolated from the surface of black plastic pieces which constitute the fluidized bed filter (onsite wastewater technology, OSWT). The preliminary isolation of the strain was done in the medium with tetracycline at a 16 mg/l concentration. To characterize the isolated strain, the following Biolog methods were applied: (1) EcoPlates microplates for evaluation of physiological profiling, (2) GEN III OmniLog® ID System for identification of the isolate, and (3) phenotypic microarrays (PM) technology for evaluation of sensitivity to antibiotics (PM11 and PM12)...
2015: Acta Biochimica Polonica
Jin-Hyeob Kwak, Scott X Chang, M Anne Naeth, Wolfgang Schaaf
Forest floor mineral soil mix (FMM) and peat mineral soil mix (PMM) are cover soils commonly used for upland reclamation post open-pit oil sands mining in northern Alberta, Canada. Coarse woody debris (CWD) can be used to regulate soil temperature and water content, to increase organic matter content, and to create microsites for the establishment of microorganisms and vegetation in upland reclamation. We studied the effects of CWD on soil microbial community level physiological profile (CLPP) and soil enzyme activities in FMM and PMM in a reclaimed landscape in the oil sands...
2015: PloS One
Chaofeng Song, Juan Lin, Xiaolong Huang, Yonghong Wu, Jiantong Liu, Chenxi Wu
Parabens are extensively used as preservatives and bactericides in personal care and other consumer products, and are commonly found in wastewater and surface water as contaminants. However, few data are currently available on the ecotoxicity of parabens. Periphyton biofilm, a widely distributed microbial aggregate of ecological importance in aquatic environment, is frequently used for water quality monitoring, ecological restoration, and toxicity assessment. In this work, the effects of butyl paraben on the development and microbial composition of periphyton biofilm was studied in a laboratory experiment for 32 days using flow through channels...
March 2016: Ecotoxicology
Emilie Bruez, Rana Haidar, Maryam T Alou, Jessica Vallance, Christophe Bertsch, Flore Mazet, Marc Fermaud, Alain Deschamps, Lucia Guerin-Dubrana, Stéphane Compant, Patrice Rey
Esca is a grapevine trunk disease (GTD) associated with different pathogenic fungi inhabiting the woody tissues. Bacteria can also be found in such tissues and they may interact with these fungal colonizers. Although such types of microbial interactions have been observed for wood diseases in many trees, this has never been studied for grapevine. In this study, the bacterial microflora of different vine status (esca-symptomatic and asymptomatic), different anatomical part (trunk and cordon) and different type of tissues (necrotic or not) have been studied...
2015: Frontiers in Microbiology
Mark Button, Kela Weber, Jaime Nivala, Thomas Aubron, Roland Arno Müller
Community-level physiological profiling (CLPP) using BIOLOG® EcoPlates™ has become a popular method for characterizing and comparing the functional diversity, functional potential, and metabolic activity of heterotrophic microbial communities. The method was originally developed for profiling soil communities; however, its usage has expanded into the fields of ecotoxicology, agronomy, and the monitoring and profiling of microbial communities in various wastewater treatment systems, including constructed wetlands for water pollution control...
March 2016: Applied Biochemistry and Biotechnology
M Jeanbille, M Buée, C Bach, A Cébron, P Frey-Klett, M P Turpault, S Uroz
Soil and climatic conditions as well as land cover and land management have been shown to strongly impact the structure and diversity of the soil bacterial communities. Here, we addressed under a same land cover the potential effect of the edaphic parameters on the soil bacterial communities, excluding potential confounding factors as climate. To do this, we characterized two natural soil sequences occurring in the Montiers experimental site. Spatially distant soil samples were collected below Fagus sylvatica tree stands to assess the effect of soil sequences on the edaphic parameters, as well as the structure and diversity of the bacterial communities...
February 2016: Microbial Ecology
Juraj Medo, Jana Maková, Silvia Kovácsová, Kamila Majerčíková, Soňa Javoreková
This investigation was undertaken to determine the impact of the insecticides Dursban 480 EC (with organophosphate compound chlorpyrifos as the active ingredient) and Talstar 10 EC (with pyrethroid bifenthrin as the active ingredient) on the respiration activity and microbial diversity in a sandy loam luvisol soil. The insecticides were applied in two doses: the maximum recommended dose for field application (15 mg kg(-1) for Dursban 480 EC and 6 mg kg(-1) for Talstar 10 EC) and a 100-fold higher dose for extrapolation of their effect...
2015: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Siying Liu, Jia Liu, Juanjuan Zhao, Dongsheng Xia, Fei Pan, Ci Liu, George Z Kyzas, Jie Fu
To investigate the effects of palygorskite on chemical forms of heavy metal and microbial functional diversity in the sewage sludge composting, a compost matrix of sewage sludge, wheat straw and varying contents of palygorskite were inoculated with the compound microbial preparation. The chemical speciation analysis by a Community Bureau of Reference-sequential extraction indicated the contribution of palygorskite to reduce the bioavailability of a model metal, Cu, during the composting process. The Biolog EcoPlate(TM) test revealed that the microbial community showed better capability of utilizing complex macro-molecules (such as miscellaneous and polymers) in the presence of palygorskite...
2015: Environmental Technology
Xiangdong Huang, Dong Xue, Lian Xue
A greenhouse experiment was conducted to investigate the impact of sewage sludge compost application on functional diversity of soil microbial communities, based on carbon source utilization, and biochemical characteristics of tree peony (Paeonia suffruticosa). Functional diversity was estimated with incubations in Biolog EcoPlates and well color development was used as the functional trait for carbon source utilization. The average well color development and Shannon index based on the carbon source utilization pattern in Biolog EcoPlates significantly increased with the increasing sludge compost application in the range of 0-45%, with a decreasing trend above 45%...
August 2015: Environmental Science and Pollution Research International
Zijun Zhou, Changwen Du, Ting Li, Yazhen Shen, Yin Zeng, Jie Du, Jianmin Zhou
Biochar-modified polyacrylate-like polymers are promising waterborne polymer-based membrane coatings for controlled-release fertilizers. However, the effect of these membrane polymers on paddy soil is unknown. A soil incubation experiment was conducted using Fourier transform infrared photoacoustic spectroscopy to monitor the changes in the polymer-coated membranes in paddy soil, and Biolog EcoPlates and polymerase chain reaction-denaturing gradient gel electrophoresis were used to detect the effects of the membranes on soil bacterial community profiles...
June 2015: Environmental Science and Pollution Research International
Yanxia Li, Bei Liu, Xuelian Zhang, Min Gao, Jing Wang
A simulated experiment of aerobic composting was conducted on swine manure to evaluate the effects of Cu at two exposure levels (200 and 2000 mg kg(-1), corresponding to low-Cu and high-Cu treatments, respectively) on the activity of microorganisms. In addition, the microbial pollution-induced community tolerance (PICT) to Cu and co-tolerance to selected antibiotics (tylosin and vancomycin) in the composted products were also investigated using the Biolog Ecoplates™ method. It was demonstrated that the enzymatic activities were significantly inhibited by the high-Cu treatment, with maximal inhibition rates of 56...
2015: Journal of Hazardous Materials
Ina Severin, Eva S Lindström, Orjan Ostman
Bacterial communities are immensely diverse and drive many fundamental ecosystem processes. However, the role of bacterial community composition (BCC) for functioning is still unclear. Here we evaluate the relative importance of BCC (from 454-sequencing), functional traits (from Biolog Ecoplates) and environmental conditions for per cell biomass production (BPC; 3H-leucine incorporation) in six data sets of natural freshwater bacterial communities. BCC explained significant variation of BPC in all six data sets and most variation in four...
2014: PloS One
C Henrik Johansson, Lisa Janmar, Thomas Backhaus
Ciprofloxacin and sulfamethoxazole are two antibiotics commonly detected in the aquatic environment, but information on their toxicity towards natural microbial communities is largely absent. In particular no data are available for marine microorganisms. The aim of the current study was therefore to evaluate the chronic toxicity of ciprofloxacin and sulfamethoxazole to natural marine biofilms (periphyton), a complex ecological community comprising a variety of bacterial and algal species. The biofilms were sampled along the Swedish west coast and subsequently exposed over 4 days in a semi-static system to a concentration series of each antibiotic...
November 2014: Aquatic Toxicology
Cláudia I Lima-Bittencourt, Patrícia S Costa, Mariana P Reis, Alexandre B Santos, Francisco A R Barbosa, Jean L Valentin, Fabiano L Thompson, Edmar Chartone-Souza, Andréa M A Nascimento
Due to the importance of heterotrophic bacteria in biogeochemical cycles and their influence on water quality, many studies have assessed the composition of the bacterial community. Most of these were made in temperate freshwaters. Eighteen heterotrophic bacteria communities distributed over time and space in the water column of Carioca Lake, not exposed to anthropogenic activities, were analyzed to characterize their composition. A polyphasic approach was used, including 16S rDNA restriction analysis, 16S rRNA gene sequence analysis, BIOLOG Ecoplates and statistical methods...
2014: PeerJ
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