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KIR4.1

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https://www.readbyqxmd.com/read/28679838/fluorocitrate-mediated-depolarization-of-astrocytes-in-the-retrotrapezoid-nucleus-stimulates-breathing
#1
Cleyton R Sobrinho, Christopher M Gonçalves, Ana C Takakura, Daniel K Mulkey, Thiago S Moreira
Evidence indicates that CO2/H(+)-evoked ATP released from retrotrapezoid nucleus (RTN) astrocytes modulates the activity of CO2-sensitive neurons. RTN astrocytes also sense H+ by inhibition of Kir4.1 channels; however, the relevance of this pH-sensitive current remains unclear since ATP release appears to involve CO2-dependent gating of connexin 26 hemichannels. Considering that depolarization mediated by H(+) inhibition of Kir4.1 channels is expected to increase sodium bicarbonate cotransporter (NBC) conductance and favor Ca(2+) influx via the sodium calcium exchanger (NCX), we hypothesize that depolarization in the presence of CO2 is sufficient to facilitate ATP release and enhance respiratory output...
July 5, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28615682/improved-retinal-function-in-rcs-rats-after-suppressing-the-over-activation-of-mglur5
#2
Jiaman Dai, Yan Fu, Yuxiao Zeng, Shiying Li, Zheng Qin Yin
Müller cells maintain retinal synaptic homeostasis by taking up glutamate from the synaptic cleft and transporting glutamine back to the neurons. To study the interaction between Müller cells and photoreceptors, we injected either DL-α-aminoadipate or L-methionine sulfoximine-both inhibitors of glutamine synthetase-subretinally in rats. Following injection, the a-wave of the electroretinogram (ERG) was attenuated, and metabotropic glutamate receptor 5 (mGluR5) was activated. Selective antagonism of mGluR5 by 2-methyl-6-(phenylethynyl)-pyridine increased the ERG a-wave amplitude and also increased rhodopsin expression...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28597918/inflammation-in-the-developing-rat-modulates-astroglial-reactivity-to-seizures-in-the-mature-brain
#3
Zuzanna Setkowicz, Emilia Kosonowska, Krzysztof Janeczko
Astrocytes participate in neuronal development and excitability, and produce factors enhancing or suppressing inflammatory processes occurring due to neurodegenerative diseases, such as epilepsy. Seizures, in turn, trigger the release of inflammatory mediators, causing structural and functional changes in the brain. Therefore, it appears reasonable to determine whether generalized inflammation at developmental periods can affect astrocyte reactivity to epileptic seizures occurring in the adult brain. Lipopolysaccharide (LPS) was injected in 6- or 30-day-old rats (P6 or P30, respectively)...
June 9, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28577853/loss-of-transcriptional-activation-of-the-potassium-channel-kir5-1-by-hnf1%C3%AE-drives-autosomal-dominant-tubulointerstitial-kidney-disease
#4
Andreas Kompatscher, Jeroen H F de Baaij, Karam Aboudehen, Anke P W M Hoefnagels, Peter Igarashi, René J M Bindels, Gertjan J C Veenstra, Joost G J Hoenderop
Hepatocyte nuclear factor 1 homeobox B (HNF1β) is an essential transcription factor for the development and functioning of the kidney. Mutations in HNF1β cause autosomal dominant tubulointerstitial kidney disease characterized by renal cysts and maturity-onset diabetes of the young (MODY). Moreover, these patients suffer from a severe electrolyte phenotype consisting of hypomagnesemia and hypokalemia. Until now, genes that are regulated by HNF1β are only partially known and do not fully explain the phenotype of the patients...
May 31, 2017: Kidney International
https://www.readbyqxmd.com/read/28576938/neuroimmune-glia-interactions-in-the-sensory-ganglia-account-for-the-development-of-acute-herpetic-neuralgia
#5
Jaqueline R Silva, Alexandre H Lopes, Jhimmy Talbot, Nerry T Cecilio, Mateus F Rossato, Rangel L Silva, Guilherme R Souza, Cassia R Silva, Guilherme Lucas, Benedito A Fonseca, Eurico Arruda, Jose C Alves-Filho, Fernando Q Cunha, Thiago M Cunha
Herpetic neuralgia is the most important symptom of herpes zoster disease, which is caused by Varicella zoster Nevertheless, the pathophysiological mechanisms involved in herpetic neuralgia are not totally elucidated. Here, we examined the neuroimmune interactions at the sensory ganglia that account for the genesis of herpetic neuralgia using a murine model of Herpes Simplex Virus Type-1 (HSV-1) infection. The cutaneous HSV-1 infection of mice results in the development of a zosteriform-like skin lesion followed by a time-dependent increase in pain-like responses (mechanical allodynia)...
July 5, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28562601/multifunctional-glial-support-by-semper-cells-in-the-drosophila-retina
#6
Mark A Charlton-Perkins, Edward D Sendler, Elke K Buschbeck, Tiffany A Cook
Glial cells play structural and functional roles central to the formation, activity and integrity of neurons throughout the nervous system. In the retina of vertebrates, the high energetic demand of photoreceptors is sustained in part by Müller glia, an intrinsic, atypical radial glia with features common to many glial subtypes. Accessory and support glial cells also exist in invertebrates, but which cells play this function in the insect retina is largely undefined. Using cell-restricted transcriptome analysis, here we show that the ommatidial cone cells (aka Semper cells) in the Drosophila compound eye are enriched for glial regulators and effectors, including signature characteristics of the vertebrate visual system...
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28548026/low-reliability-of-anti-kir4-183-120-peptide-auto-antibodies-in-multiple-sclerosis-patients
#7
Mariapaola Marino, Giovanni Frisullo, Gabriele Di Sante, Daniela Maria Samengo, Carlo Provenzano, Massimiliano Mirabella, Giovambattista Pani, Francesco Ria, Emanuela Bartoccioni
BACKGROUND: Multiple sclerosis (MS) is an autoimmune disease for which auto-antibodies fully validated as diagnostic and prognostic biomarkers are widely desired. Recently, an immunoreactivity against the inward rectifying potassium channel 4.1 (KIR4.1) has been reported in a large proportion of a group of MS patients, with amino acids 83-120 being the major epitope. Moreover, a strong correlation between anti-KIR4.183-120 and anti-full-length-protein auto-antibodies titer was reported...
May 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28526685/differences-in-molecular-mechanisms-of-k-clearance-in-the-auditory-sensory-epithelium-of-birds-and-mammals
#8
Viviane Wilms, Chris Söffgen, Hans Gerd Nothwang
Mechanoelectrical transduction in the vertebrate inner ear is a highly conserved mechanism depending on K(+) influx into hair cells. Here, we investigated the molecular underpinnings of subsequent K(+) recycling in the chicken basilar papilla and compared it with those in the mammalian auditory sensory epithelium. Like mammals, the avian auditory hair cell uses KCNQ4, KCNMA1, and KCNMB1 as K(+) efflux systems. Expression of KCNQ1 and KCNE1 suggests an additional efflux apparatus in avian hair cells. Marked differences were observed for K(+) clearance...
May 19, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28520217/the-gene-encoding-the-inwardly-rectifying-potassium-channel-kir4-1-may-be-involved-in-sudden-infant-death-syndrome
#9
Siri H Opdal, Åshild Vege, Arne Stray-Pedersen, Torleiv O Rognum
AIM: Disturbances in brain function and development may play a role in sudden infant death syndrome (SIDS). This Norwegian study aimed to test the hypothesis that specific variants of genes involved in water transport and potassium homeostasis would be predisposing factors for SIDS. METHODS: Genetic variation in the genes encoding aquaporin-4 (AQP4), Kir4.1 (KCNJ10) and α-syntrophin were analysed in 171 SIDS cases (62.6% male) with a median age of 15.5 (2-52) weeks and 398 adult controls (70...
May 18, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28460049/tumor-necrosis-factor-alpha-tnf-%C3%AE-disrupts-kir4-1-channel-expression-resulting-in-m%C3%A3-ller-cell-dysfunction-in-the-retina
#10
Iraj Hassan, Qianyi Luo, Sreeparna Majumdar, James M Dominguez, Julia V Busik, Ashay D Bhatwadekar
Purpose: Diabetic patients often are affected by vision problems. We previously identified diabetic retinopathy (DR) as a disease of clock gene dysregulation. TNF-α, a proinflammatory cytokine, is known to be elevated in DR. Müller cells maintain retinal water homeostasis and K+ concentration via Kir4.1 channels. Notably, Kir4.1 expression is reduced in diabetes; however, the interplay of TNF-α, Kir4.1, and clock genes in Müller cells remains unknown. We hypothesize that the Kir4...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28445150/modulation-of-the-inwardly-rectifying-potassium-channel-kir4-1-by-the-pro-invasive-mir-5096-in-glioblastoma-cells
#11
Dominique Thuringer, Gaetan Chanteloup, Jonathan Boucher, Nicolas Pernet, Christophe Boudesco, Gaetan Jego, Aurelien Chatelier, Patrick Bois, Jessica Gobbo, Laurent Cronier, Eric Solary, Carmen Garrido
Inwardly rectifying potassium channels (Kir), and especially the barium-sensitive Kir4.1 encoded by KCNJ10, are key regulators of glial functions. A lower expression or mislocation of Kir4.1 is detected in human brain tumors. MicroRNAs participate in the regulation of ionic channels and associated neurologic disorders. Here, we analyze effects of miR-5096 on the Kir4.1 expression and function in two glioblastoma cell lines, U87 and U251. Using whole-cell patch-clamp and western-blot analysis, we show that cell loading with miR-5096 decreases the Kir4...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28414733/absence-of-antibodies-against-kir4-1-in-multiple-sclerosis-a-three-technique-approach-and-systematic-review
#12
Miquel Navas-Madroñal, Ana Valero-Mut, María José Martínez-Zapata, Manuel Javier Simón-Talero, Sebastián Figueroa, Nuria Vidal-Fernández, Mariana López-Góngora, Antonio Escartín, Luis Querol
INTRODUCTION: Antibodies targeting the inward-rectifying potassium channel KIR4.1 have been associated with multiple sclerosis (MS) but studies using diverse techniques have failed to replicate this association. The detection of these antibodies is challenging; KIR4.1 glycosylation patterns and the use of diverse technical approaches may account for the disparity of results. We aimed to replicate the association using three different approaches to overcome the technical limitations of a single technique...
2017: PloS One
https://www.readbyqxmd.com/read/28395711/-il-1%C3%AE-promotes-the-proliferation-of-astrocytes-and-downregulates-the-expression-of-kir4-1
#13
Meiqun Sun, Hongtao Wang, Qi Qi, Haiqin Yan, Weiyan Zou, Xingwu Dong, Zihai Wang, Jinghao Wang, Xiaojing Wang
Objective To explore the impact of IL-1β on the proliferation of astrocytes and the expression of the inwardly rectifying potassium channel 4.1 (Kir4.1) in astrocytes. Methods Astrocytes were isolated from cerebral cortex of newborn SD rats and cultured in the presence of IL-1β or IL-1β combined with interleukin-1 receptor antagonist (IL-1Ra). The effect of IL-1β on the cell cycle of astrocytes was measured with flow cytometry; the level of Kir4.1 mRNA was determined by quantitative real-time PCR. The level of Kir4...
April 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28391264/anti-kir4-1-antibodies-in-chinese-patients-with-central-nervous-system-inflammatory-demyelinating-disorders
#14
Rong Zhong, Junyan Liang, Ailin Tao, Linzhan Wu, Xinguang Yang, Huiming Xu, Qingmei Huang, Shunzhi Zhuang, Youming Long, Cong Gao
OBJECTIVES: The aim of this study was to explore the frequency of KIR4.1 antibodies in patients with multiple sclerosis (MS) and in control groups using a cell-based assay. MATERIALS AND METHODS: A transfected HEK-293A cell line expressing KIR4.1 was established to test for the presence of KIR4.1 antibodies in blood serum. We tested 904 subjects, including 188 patients with MS, 264 patients with neuromyelitis optica spectrum disorders (NMOSD), 209 patients with other inflammatory neurologic disease (OIND), 203 patients with other noninflammatory neurological disease (OND), and 40 healthy controls...
2016: Neuroimmunomodulation
https://www.readbyqxmd.com/read/28365586/enac-and-romk-activity-are-inhibited-in-the-dct2-cnt-of-tgwnk4-phaii-mice
#15
Chengbiao Zhang, Lijun Wang, Xiao-Tong Su, Junhui Zhang, Dao-Hong Lin, Wen-Hui Wang
Mice transgenic for genomic segments harboring PHAII (pseudohypoaldosteronism type II) mutant Wnk4 (with-No-Lysine kinase 4) (TgWnk4(PHAII)) have hyperkalemia which is currently believed to be the result of high activity of Na-Cl cotransporter (NCC). This leads to decreasing Na(+) delivery to the distal nephron segment including late distal convoluted tubule (DCT) and connecting tubule (CNT). Since epithelial Na(+) channel (ENaC) and renal outer medullary K(+) channel (ROMK or Kir4.1) are expressed in the late DCT and play an important role in mediating K(+) secretion, the aim of the present study is to test whether ROMK and ENaC activity in the DCT/CNT are also compromised in the mice expressing PHAII mutant Wnk4...
April 1, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28345117/inwardly-rectifying-k-currents-in-cultured-oligodendrocytes-from-rat-optic-nerve-are-insensitive-to-ph
#16
Alberto Pérez-Samartín, Edith Garay, Juan Pablo H Moctezuma, Abraham Cisneros-Mejorado, María Victoria Sánchez-Gómez, Guadalupe Martel-Gallegos, Leticia Robles-Martínez, Manuel Canedo-Antelo, Carlos Matute, Rogelio O Arellano
Inwardly rectifying K(+) (Kir) channel expression signals at an advanced stage of maturation during oligodendroglial differentiation. Knocking down their expression halts the generation of myelin and produces severe abnormalities in the central nervous system. Kir4.1 is the main subunit involved in the tetrameric structure of Kir channels in glial cells; however, the precise composition of Kir channels expressed in oligodendrocytes (OLs) remains partially unknown, as participation of other subunits has been proposed...
March 27, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28300540/detection-of-potassium-channel-kir4-1-antibodies-in-multiple-sclerosis-patients
#17
Fabiana Marnetto, Paola Valentino, Marzia Caldano, Antonio Bertolotto
The presence of KIR4.1 antibodies has been proposed to be a characteristic of Multiple Sclerosis (MS). This could have a significant impact on disease management. However, the validation of the initial findings has failed till date. Conflicting results have been attributed to difficulties in isolating the lower-glycosylated (LG) KIR4.1 expressed in oligodendrocytes, the putative target antigen of autoantibodies. The aim of this study is to verify the presence of KIR4.1 antibodies in MS patients, by independently replicating the originally-described procedure...
March 12, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28297725/rearing-light-intensity-affects-inner-retinal-pathology-in-a-mouse-model-of-x-linked-retinoschisis-but-does-not-alter-gene-therapy-outcome
#18
Dario Marangoni, Zeng Yong, Sten Kjellström, Camasamudram Vijayasarathy, Paul A Sieving, Ronald A Bush
Purpose: To test the effects of rearing light intensity on retinal function and morphology in the retinoschisis knockout (Rs1-KO) mouse model of X-linked retinoschisis, and whether it affects functional outcome of RS1 gene replacement. Methods: Seventy-six Rs1-KO mice were reared in either cyclic low light (LL, 20 lux) or moderate light (ML, 300 lux) and analyzed at 1 and 4 months. Retinal function was assessed by electroretinogram and cavity size by optical coherence tomography...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28288868/inhibition-of-kir4-1-potassium-channels-by-quinacrine
#19
Leticia G Marmolejo-Murillo, Iván A Aréchiga-Figueroa, Meng Cui, Eloy G Moreno-Galindo, Ricardo A Navarro-Polanco, José A Sánchez-Chapula, Tania Ferrer, Aldo A Rodríguez-Menchaca
Inwardly rectifying potassium (Kir) channels are expressed in many cell types and contribute to a wide range of physiological processes. Particularly, Kir4.1 channels are involved in the astroglial spatial potassium buffering. In this work, we examined the effects of the cationic amphiphilic drug quinacrine on Kir4.1 channels heterologously expressed in HEK293 cells, employing the patch clamp technique. Quinacrine inhibited the currents of Kir4.1 channels in a concentration and voltage dependent manner. In inside-out patches, quinacrine inhibited Kir4...
May 15, 2017: Brain Research
https://www.readbyqxmd.com/read/28270749/identifying-candidate-genes-that-underlie-cellular-ph-sensitivity-in-serotonin-neurons-using-transcriptomics-a-potential-role-for-kir5-1-channels
#20
Madeleine M Puissant, Gary C Mouradian, Pengyuan Liu, Matthew R Hodges
Ventilation is continuously adjusted by a neural network to maintain blood gases and pH. Acute CO2 and/or pH regulation requires neural feedback from brainstem cells that encode CO2/pH to modulate ventilation, including but not limited to brainstem serotonin (5-HT) neurons. Brainstem 5-HT neurons modulate ventilation and are stimulated by hypercapnic acidosis, the sensitivity of which increases with increasing postnatal age. The proper function of brainstem 5-HT neurons, particularly during post-natal development is critical given that multiple abnormalities in the 5-HT system have been identified in victims of Sudden Infant Death Syndrome...
2017: Frontiers in Cellular Neuroscience
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