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Mucin 5ac eye

Dhruva Bhattacharya, Li Yu, Mingwu Wang
The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC...
2017: PloS One
Yuichi Hori, Tomofumi Kageyama, Asuka Sakamoto, Tomoaki Shiba, Masatsugu Nakamura, Takatoshi Maeno
PURPOSE: To investigate the short-term effects of 2 new secretagogue eye drops for dry eye, 3% diquafosol tetrasodium ophthalmic solution (diquafosol) and 2% rebamipide ophthalmic suspension (rebamipide), on the concentration of mucin 5AC (MUC5AC) in rabbit tear fluid and conjunctival goblet cells. METHODS: One dose of artificial tears, diquafosol or rebamipide, was instilled into 8 eyes of Japanese white rabbits. MUC5AC concentration in the tear fluid was examined using the enzyme-linked immunosorbent assay 15 min after instillation and compared with 8 untreated controls...
July 2017: Journal of Ocular Pharmacology and Therapeutics
Takahiro Nakamura, Yuiko Hata, Maho Nagata, Norihiko Yokoi, Shumpei Yamaguchi, Taiichi Kaku, Shigeru Kinoshita
Dry eye syndrome (DES), a multifactorial disease of the tears and ocular surface, is one of the most common ocular disorders. Tear film contains ocular mucins and is essential for maintaining the homeostasis of the wet ocular surface. Since there are a limited number of clinical options for the treatment of DES, additional novel treatments are needed to improve the clinical results. In this study, we found that placental extract-derived dipeptide (JBP485) clearly promoted the expression and secretion of gel-forming mucin 5ac (Muc5ac) in rabbit conjunctival epithelium...
2015: Scientific Reports
Takashi Kojima, Murat Dogru, Osama M Ibrahim, Taeko Nagata, Kazunari Higa, Takahiko Shimizu, Takuji Shirasawa, Yoshiyuki Satake, Seika Shimazaki, Jun Shimazaki, Kazuo Tsubota
PURPOSE: To investigate the role of a water and mucin secretagogue (3% diquafosol sodium eye drops) on the tear function and conjunctival ocular surface changes in Sod1(-/-) in comparison to the wild-type (WT) mice. METHODS: Fourteen eyes of 7 Sod1(-/-) male mice with C57BL/background and 14 eyes of 7 C57BL6 strain wild-type male mice were examined at 40 weeks in this study. All mice had application of 3% diquafosol ophthalmic solution six times a day for 2 weeks...
2014: Molecular Vision
Yuichi Uchino, Miki Uchino, Norihiko Yokoi, Murat Dogru, Motoko Kawashima, Naoko Okada, Takaaki Inaba, Shusaku Tamaki, Aoi Komuro, Yukiko Sonomura, Hiroaki Kato, Pablo Argüeso, Shigeru Kinoshita, Kazuo Tsubota
IMPORTANCE: There are limited reports on the relationship between mucin 5AC (MUC5AC) concentrations in tears, working hours, and the frequency of ocular symptoms in visual display terminal (VDT) users. This investigation evaluated these relationships among patients with dry eye disease (DED) and individuals serving as controls. OBJECTIVE: To determine the relationship between MUC5AC concentration in the tears of VDT users based on the diagnosis of DED and frequency of ocular symptoms...
August 2014: JAMA Ophthalmology
Rieko Arakaki, Hiroshi Eguchi, Akiko Yamada, Yasusei Kudo, Akihiko Iwasa, Tserennadmid Enkhmaa, Fumika Hotta, Sayaka Mitamura-Aizawa, Yoshinori Mitamura, Yoshio Hayashi, Naozumi Ishimaru
BACKGROUND: Topical therapy is effective for dry eye, and its prolonged effects should help in maintaining the quality of life of patients with dry eye. We previously reported that the oral administration of rebamipide (Reb), a mucosal protective agent, had a potent therapeutic effect on autoimmune lesions in a murine model of Sjögren's syndrome (SS). However, the effects of topical treatment with Reb eyedrops on the ocular lesions in the murine model of SS are unknown. METHODS AND FINDING: Reb eyedrops were administered to the murine model of SS aged 4-8 weeks four times daily...
2014: PloS One
André Augusto Miranda Torricelli, Monique Matsuda, Priscila Novaes, Alfésio Luiz Ferreira Braga, Paulo Hilário Nascimento Saldiva, Milton Ruiz Alves, Mário Luiz Ribeiro Monteiro
PURPOSE: To quantify ocular symptoms, goblet cells (GC) and mucin 5AC (MUC5AC) gene expression on the conjunctiva of healthy subjects exposed to ambient levels of traffic-derived air pollution and to estimate its correlation with NO2 and particulate matter smaller than 2.5 μm (PM2.5) levels. METHODS: Twenty-one taxi drivers or traffic controllers were assessed with the Ocular Surface Disease Index (OSDI) questionnaire and conjunctival impression cytology. MUC5AC mRNA levels were determined based on the cytology of the right eye, and GC density was assessed based on the cytology of the left eye...
May 2014: Environmental Research
Hyung Bin Hwang, Hyun Seung Kim
PURPOSE: We evaluated light exposure-induced dry eye syndrome by investigating the phototoxic effects of an operating microscope on the ocular surface and tear film in rabbits. METHODS: Sixty eyes of 30 rabbits were divided into 3 groups based on the intensity of light exposure received from an operating microscope: Control group, no exposure to light; group A, 40,000-lx intensity for 30 minutes; and group B, 100,000-lx intensity for 30 minutes. To evaluate the potential damage to the ocular surface and tear film, Schirmer tests, rose bengal staining, and conjunctival impression cytology were performed before the light exposure and at 1, 3, and 5 days afterward...
January 2014: Cornea
Takeshi Ohguchi, Takashi Kojima, Osama M Ibrahim, Taeko Nagata, Takahiko Shimizu, Takuji Shirasawa, Tetsuya Kawakita, Yoshiyuki Satake, Kazuo Tsubota, Jun Shimazaki, Susumu Ishida
PURPOSE: To investigate the efficacy of 2% rebamipide ophthalmic solution on the tear functions and ocular surface status of the superoxide dismutase-1(Sod1(-/-)) mice. METHODS: Two percent Rebamipide ophthalmic solution was applied to 40-week-old male Sod1(-/-) and wild-type (WT) mice four times a day for 2 weeks. We examined the cytokine concentrations in the tear fluid (by CytoBead assay), tear film break-up time, amount of tear production, and expressions of mucins 1, 4, and 5AC, by RT-PCR...
November 21, 2013: Investigative Ophthalmology & Visual Science
Jianan Zhang, Xiaoming Yan, Haili Li
PURPOSE: To investigate the expression of mucin and inflammatory factors in the conjunctival epithelium of subjects with dry eye and determine a correlation between clinical laboratory tests and subjective symptoms. METHODS: The ocular surface disease index questionnaire, corneal fluorescein staining, tear film breakup time, and Schirmer I tests were administered in 66 subjects. According to questionnaire scores, the subjects were divided into mild, moderate, and severe symptom groups...
July 2013: Cornea
Anne M Floyd, Xu Zhou, Christopher Evans, Olivia J Rompala, Lingxiang Zhu, Mingwu Wang, Yin Chen
MUC5AC is the most abundant gel-forming mucin in the ocular system. However, the specific function is unknown. In the present study, a Muc5ac knockout (KO) mouse model was subject to various physiological measurements as compared to its wide-type (WT) control. Interestingly, when KO mice were compared to WT mice, the mean tear break up time (TBUT) values were significantly lower and corneal fluorescein staining scores were significantly higher. But the tear volume was not changed. Despite the lack of Muc5ac expression in the conjunctiva of KO mice, Muc5b expression was significantly increased in these mice...
2012: PloS One
Hong Liang, Christophe Baudouin, Philippe Daull, Jean-Sébastien Garrigue, Ronald Buggage, Françoise Brignole-Baudouin
PURPOSE: Cationic emulsions (CEs), developed as vehicles for lipophilic drugs, have been shown to be safe and effective for the treatment of dry eye. The aim of this study was to investigate the effects of a preservative-free latanoprost 0.005% CE (latanoprost-CE) in in vitro and in vivo models of corneal wound healing. METHODS: An in vitro wound was made by scraping through a confluent layer of human corneal epithelial cells. Cytotoxicity, cell migration, and proliferation were analyzed after an exposure to phosphate-buffered saline, CE, latanoprost-CE, 0...
November 2012: Cornea
José Reinaldo S Ricardo, Priscila C Cristovam, Pedro A N Filho, Charles C Farias, Aline L de Araujo, Renata R Loureiro, Joyce L Covre, Jeison N de Barros, Telma P Barreiro, Myrna S dos Santos, José A P Gomes
PURPOSE: To report the outcomes of transplantation of autologous conjunctival epithelial cells cultivated ex vivo (EVCAU) in patients with total limbal stem cell deficiency (LSCD). METHODS: EVCAU were cultivated on denuded human amniotic membrane and transplanted in 12 eyes of 10 patients with total LSCD. We evaluated the improvement in the defined clinical parameters of LSCD (loss of corneal epithelial transparency, superficial corneal neovascularization and epithelial irregularity/recurrent epithelial breakdown), vision acuity, impression cytology, immunocytochemical analysis (CK3/CK19), and the appearance of a regular hexagonal basal layer of cells on corneal confocal microscopy...
March 2013: Cornea
Hong Liang, Françoise Brignole-Baudouin, Luisa Riancho, Christophe Baudouin
The study used a validated acute in vivo model to compare a new formulation of travoprost 0.004% ophthalmic solution(travoprost PQ), preserved with polyquaternium-1 (PQ), with commercially available formulations of benzalkonium-chloride(BAK)-preserved travoprost 0.004% ophthalmic solution(travoprost BAK) and BAK-preserved latanoprost 0.005%ophthalmic solution (latanoprost BAK). Adult male New Zealand albino rabbits (n = 36) were randomly divided into 6 groups. Phosphate-buffered saline (PBS), 0.001% PQ, 0.015% BAK, travoprost PQ, travoprost BAK or latanoprost BAK were applied onto rabbit eyes as 1 drop, for 15 times at 5-min intervals...
2012: Ophthalmic Research
Hong Liang, Christophe Baudouin, Antoine Labbe, Luisa Riancho, Françoise Brignole-Baudouin
Conjunctiva-associated lymphoid tissue (CALT) is closely associated with ocular surface immunity. This study investigated the effects of antiglaucoma prostaglandin analogs with or without benzalkonium chloride (BAK) preservative on organized CALT using an acute toxic model. A total of 48 albino rabbits were used and seven groups of treatments were constituted. Solutions (50 µl) of PBS, 0.02%BAK, (0.02%BAK+)latanoprost, (0.015%BAK+)travoprost, (0.005%BAK+)bimatoprost, (BAK-free)travoprost preserved with the SofZia® system or (BAK-free)tafluprost were instilled 15 times at 5-min intervals in both eyes...
2012: PloS One
Chaoyang Li, Yiyue Song, Shaohong Luan, Pengxia Wan, Naiyang Li, Jing Tang, Yu Han, Cuiju Xiong, Zhichong Wang
BACKGROUND: Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium chloride (BAC) and determined the valid time of this model. METHODS AND FINDINGS: Eighty white rabbits were randomly divided into four groups...
2012: PloS One
Nadja Knop, Donald R Korb, Caroline A Blackie, Erich Knop
PURPOSE: The conjunctival side of the upper and lower inner eyelid borders, termed the lid wiper, has a thickened epithelial lip for apposition to the globe, assumed to distribute the preocular tear film. The human lid wiper structure and its goblet cells are investigated. METHODS: Conjunctival whole mounts, including lid margins from 17 eyes of human body donors, were investigated by routine histology and semithin plastic sections, using histology, histochemistry, and immunohistochemistry...
June 2012: Cornea
Iker Garcia, Jaime Etxebarria, Ana Boto-de-Los-Bueis, David Díaz-Valle, Luis Rivas, Itziar Martínez-Soroa, Nerea Saenz, Carlos López, Almudena Del-Hierro-Zarzuelo, Rosa Méndez, Javier Soria, Nerea González, Tatiana Suárez, Arantxa Acera
PURPOSE: To evaluate a limbal stem cell deficiency (LSCD) diagnosis method based on the detection of the MUC5AC transcript by reverse transcription-polymerase chain reaction (RT-PCR) in comparison with the standard diagnostic method based on goblet cell detection by periodic acid-Schiff (PAS)-hematoxylin staining, using samples obtained from corneal epithelium impression cytology (IC). DESIGN: Transversal, comparative case series. PARTICIPANTS: We studied 59 eyes from 43 patients clinically diagnosed with LSCD...
May 2012: Ophthalmology
Ilene K Gipson, Sandra J Spurr-Michaud, Michelle Senchyna, Robert Ritter, Debra Schaumberg
PURPOSE: To determine if levels of the glycocalyx membrane mucins, MUC1 and MUC16, and the secreted goblet cell mucin MUC5AC are altered in conjunctival cells and tears of postmenopausal women presenting with a history of non-Sjögren dry eye and if mucin levels correlate with dry eye clinical diagnostic data. METHODS: Eighty-four postmenopausal women with a history of non-Sjögren dry eye and 30 normal subjects were recruited for this study. Impression cytology samples were collected for mucin messenger RNA (mRNA) and protein analysis...
December 2011: Cornea
Rosa M Corrales, Srihari Narayanan, Itziar Fernández, Agustín Mayo, David J Galarreta, Graciana Fuentes-Páez, Felipe J Chaves, José M Herreras, Margarita Calonge
PURPOSE: To evaluate mRNA levels of the ocular mucins MUC1, MUC2, MUC4, MUC5AC, and MUC7 in conjunctival impression cytology samples from patients with moderate to severe dry eye syndrome (DES) compared with a population of healthy subjects; and to investigate the use of the levels of these mucin genes as biomarkers of DES and subsequently as a potential diagnostic test for DES. METHODS: This prospective study commenced in the year 2000 and ended in the year 2009...
October 2011: Investigative Ophthalmology & Visual Science
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