keyword
MENU ▼
Read by QxMD icon Read
search

Chaperones

keyword
https://www.readbyqxmd.com/read/29653436/a-chloroplast-targeted-cabbage-dead-box-rna-helicase-brrh22-confers-abiotic-stress-tolerance-to-transgenic-arabidopsis-plants-by-affecting-translation-of-chloroplast-transcripts
#1
Ghazala Nawaz, Kwanuk Lee, Su Jung Park, Yeon-Ok Kim, Hunseung Kang
Although the roles of many DEAD-box RNA helicases (RHs) have been determined in the nucleus as well as in cytoplasm during stress responses, the importance of chloroplast-targeted DEAD-box RHs in stress response remains largely unknown. In this study, we determined the function of BrRH22, a chloroplast-targeted DEAD-box RH in cabbage (Brassica rapa), in abiotic stress responses. The expression of BrRH22 was markedly increased by drought, heat, salt, or cold stress and by ABA treatment, but was largely decreased by UV stress...
April 7, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29651925/heptamer-peptide-disassembles-native-amyloid-in-human-plasma-via-heat-shock-protein-70
#2
Timothy James Cunningham, Jeffrey I Greenstein, Lihua Yao, Itzhak Fischer, Theresa Conners
Proteostasis, which includes the repair and disposal of misfolded proteins, depends in part on the activity of heat shock proteins, a well-known class of chaperone molecules. When this process fails abnormally folded proteins may accumulate in cells, tissues, and blood. These species are a hallmark of protein aggregation diseases but also amass during aging, often in the absence of an identified clinical disorder. We report that a neuroprotective cyclic heptapeptide, CHEC-7, which has been applied systemically as a therapeutic in animal neurodegeneration models, disrupts such aggregates and inhibits amyloidogenesis when added in nanomolar concentrations to human plasma...
April 13, 2018: Rejuvenation Research
https://www.readbyqxmd.com/read/29651044/yeast-aconitase-mitochondrial-import-is-modulated-by-interactions-of-its-c-and-n-terminal-domains-and-ssa1-2-hsp70
#3
Reut Ben-Menachem, Katherine Wang, Orly Marcu, Zhang Yu, Teck Kwang Lim, Qingsong Lin, Ora Schueler-Furman, Ophry Pines
Molecules of single proteins, echoforms, can be distributed between two (or more) subcellular locations, a phenomenon which we refer to as dual targeting or dual localization. The yeast aconitase gene ACO1 (778 amino acids), encodes a single translation product that is nonetheless dual localized to the cytosol and mitochondria by a reverse translocation mechanism. The solved crystal structure of aconitase isolated from porcine heart mitochondria shows that it has four domains. The first three tightly associated N-terminal domains are tethered to the larger C-terminal fourth domain (C-terminal amino acids 517-778)...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29650969/akaps-pka-disruptors-increase-aqp2-activity-independently-of-vasopressin-in-a-model-of-nephrogenic-diabetes-insipidus
#4
Fumiaki Ando, Shuichi Mori, Naofumi Yui, Tetsuji Morimoto, Naohiro Nomura, Eisei Sohara, Tatemitsu Rai, Sei Sasaki, Yoshiaki Kondo, Hiroyuki Kagechika, Shinichi Uchida
Congenital nephrogenic diabetes insipidus (NDI) is characterized by the inability of the kidney to concentrate urine. Congenital NDI is mainly caused by loss-of-function mutations in the vasopressin type 2 receptor (V2R), leading to impaired aquaporin-2 (AQP2) water channel activity. So far, treatment options of congenital NDI either by rescuing mutant V2R with chemical chaperones or by elevating cyclic adenosine monophosphate (cAMP) levels have failed to yield effective therapies. Here we show that inhibition of A-kinase anchoring proteins (AKAPs) binding to PKA increases PKA activity and activates AQP2 channels in cortical collecting duct cells...
April 12, 2018: Nature Communications
https://www.readbyqxmd.com/read/29650953/hsp27-is-a-partner-of-jak2-stat5-and-a-potential-therapeutic-target-in-myelofibrosis
#5
Margaux Sevin, Lucia Kubovcakova, Nicolas Pernet, Sébastien Causse, Franck Vitte, Jean Luc Villeval, Catherine Lacout, Marine Cordonnier, Fernando Rodrigues-Lima, Gaétan Chanteloup, Matthieu Mosca, Marie-Lorraine Chrétien, Jean Noël Bastie, Sylvain Audia, Paul Sagot, Selim Ramla, Laurent Martin, Martin Gleave, Valérie Mezger, Radek Skoda, Isabelle Plo, Carmen Garrido, François Girodon, Aurélie de Thonel
Heat shock protein 27 (HSP27/HSPB1) is a stress-inducible chaperone that facilitates cancer development by its proliferative and anti-apoptotic functions. The OGX-427 antisense oligonucleotide against HSP27 has been reported to be beneficial against idiopathic pulmonary fibrosis. Here we show that OGX-427 is effective in two murine models of thrombopoietin- and JAKV617F-induced myelofibrosis. OGX-427 limits disease progression and is associated with a reduction in spleen weight, in megakaryocyte expansion and, for the JAKV617F model, in fibrosis...
April 12, 2018: Nature Communications
https://www.readbyqxmd.com/read/29650757/conformational-flexibility-within-the-nascent-polypeptide-associated-complex-enables-its-interactions-with-structurally-diverse-client-proteins
#6
Esther M Martin, Matthew P Jackson, Martin Gamerdinger, Karina Gense, Theodoros K Karamanos, Julia R Humes, Elke Deuerling, Alison E Ashcroft, Sheena E Radford
As newly synthesized polypeptides emerge from the ribosome, it is crucial that they fold correctly. To prevent premature aggregation, nascent chains interact with chaperones that facilitate folding or prevent misfolding until protein synthesis is complete. Nascent polypeptide-associated complex (NAC) is a ribosome-associated chaperone important for protein homeostasis. However, how NAC binds its substrates remains unclear. Using native electrospray ionization MS (ESI MS), limited proteolysis, NMR and cross-linking, we analysed the conformational properties of NAC from Caenorhabditis elegans and studied its ability to bind proteins in different conformational states...
April 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29650752/lipid-transfer-proteins-in-the-assembly-of-apob-containing-lipoproteins
#7
Alaa Sirwi, M Mahmood Hussain
A better understanding of intracellular lipoprotein assembly may help identify proteins with important roles in lipid disorders. ApoB-containing lipoproteins are macromolecular lipid and protein micelles that act as specialized transport vehicles for hydrophobic lipids. They are assembled predominantly in enterocytes and hepatocytes to transport dietary and endogenous fat, respectively, to different tissues. Assembly occurs in the endoplasmic reticulum and is dependent on lipid re-synthesis in the endoplasmic reticulum and on a chaperone, namely microsomal triglyceride transfer protein...
April 12, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29650159/expression-of-clavata3-fusions-indicates-rapid-intracellular-processing-and-a-role-of-erad
#8
Francesca De Marchis, Sara Colanero, Eva M Klein, Davide Mainieri, Viviana M Prota, Michele Bellucci, Giampiero Pagliuca, Elisa Zironi, Teresa Gazzotti, Alessandro Vitale, Andrea Pompa
The 12 amino acid peptide derived from the Arabidopsis soluble secretory protein CLAVATA3 (CLV3) acts at the cell surface in a signalling system that regulates the size of apical meristems. The subcellular pathway involved in releasing the peptide from its precursor is unknown. We show that a CLV3-GFP fusion expressed in transfected tobacco protoplasts or transgenic tobacco plants has very short intracellular half-life that cannot be extended by the secretory traffic inhibitors brefeldin A and wortmannin. The fusion is biologically active, since the incubation medium of protoplasts from CLV3-GFP-expressing tobacco contains the CLV3 peptide and inhibits root growth...
June 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29649621/astrocyte-specific-dj-1-overexpression-protects-against-rotenone-induced-neurotoxicity-in-a-rat-model-of-parkinson-s-disease
#9
Briana R De Miranda, Emily M Rocha, Qing Bai, Amina El Ayadi, David Hinkle, Edward A Burton, J Timothy Greenamyre
DJ-1 is a redox-sensitive protein with several putative functions important in mitochondrial physiology, protein transcription, proteasome regulation, and chaperone activity. High levels of DJ-1 immunoreactivity are reported in astrocytes surrounding pathology associated with idiopathic Parkinson's disease, possibly reflecting the glial response to oxidative damage. Previous studies showed that astrocytic over-expression of DJ-1 in vitro prevented oxidative stress and mitochondrial dysfunction in primary neurons...
April 9, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29649333/soluble-t-cell-receptor-design-influences-functional-yield-in-an-e-coli-chaperone-assisted-expression-system
#10
Kristin Støen Gunnarsen, Lene Støkken Høydahl, Ralf Stefan Neumann, Kaare Bjerregaard-Andersen, Nicolay Rustad Nilssen, Ludvig Magne Sollid, Inger Sandlie, Geir Åge Løset
There is a quest for production of soluble protein of high quality for the study of T-cell receptors (TCRs), but expression often results in low yields of functional molecules. In this study, we used an E. coli chaperone-assisted periplasmic production system and compared expression of 4 different soluble TCR formats: single-chain TCR (scTCR), two different disulfide-linked TCR (dsTCR) formats, and chimeric Fab (cFab). A stabilized version of scTCR was also included. Additionally, we evaluated the influence of host (XL1-Blue or RosettaBlueTM) and the effect of IPTG induction on expression profiles...
2018: PloS One
https://www.readbyqxmd.com/read/29649284/sulfasalazine-an-inhibitor-of-the-cystine-glutamate-antiporter-reduces-dna-damage-repair-and-enhances-radiosensitivity-in-murine-b16f10-melanoma
#11
Masaki Nagane, Eiichi Kanai, Yuki Shibata, Takuto Shimizu, Chie Yoshioka, Takuya Maruo, Tadashi Yamashita
The sodium-independent cystine-glutamate antiporter plays an important role in extracellular cystine uptake. It comprises the transmembrane protein, xCT and its chaperone, CD98. Because glutathione is only weakly cell membrane permeable, cellular uptake of its precursor, cystine, is known to be a key step in glutathione synthesis. Moreover, it has been reported that xCT expression affects the progression of tumors and their resistance to therapy. Sulfasalazine is an inhibitor of xCT that is known to increase cellular oxidative stress, giving it anti-tumor potential...
2018: PloS One
https://www.readbyqxmd.com/read/29643986/ajuba-receptor-mediates-the-internalization-of-tumor-secreted-grp78-into-macrophages-through-different-endocytosis-pathways
#12
Xiaoqin La, Lichao Zhang, Hanqing Li, Zhuoyu Li, Guisheng Song, Peng Yang, Yufei Yang
Glucose-regulated protein 78 (GRP78), an ER chaperone, is overexpressed in cancer cells. Solid tumor cells can secrete GRP78 that can promote tumor angiogenesis, differentiation of bone marrow-derived mesenchymal stem cells, tumor cell proliferation and polarization of tumor-associated macrophages. However, the mechanism by which GRP78 functions as a tumor promoter either by staying on the membrane to stimulate intracellular signals or directly entering into cytosolic remains unknown. Here, we reported that an endotoxin-free His-GRP78 protein was purified in vitro that simulates original secreted GRP78...
March 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29643454/activation-of-the-dnak-clpb-complex-is-regulated-by-the-properties-of-the-bound-substrate
#13
Jose Angel Fernández-Higuero, Alejandra Aguado, Judit Perales-Calvo, Fernando Moro, Arturo Muga
The chaperone ClpB in bacteria is responsible for the reactivation of aggregated proteins in collaboration with the DnaK system. Association of these chaperones at the aggregate surface stimulates ATP hydrolysis, which mediates substrate remodeling. However, a question that remains unanswered is whether the bichaperone complex can be selectively activated by substrates that require remodeling. We find that large aggregates or bulky, native-like substrates activates the complex, whereas a smaller, permanently unfolded protein or extended, short peptides fail to stimulate it...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29643123/intragenic-transcriptional-interference-regulates-the-human-immune-ligand-mica
#14
Da Lin, Thomas K Hiron, Christopher A O'Callaghan
Many human genes have tandem promoters driving overlapping transcription, but the value of this distributed promoter configuration is generally unclear. Here we show that MICA , a gene encoding a ligand for the activating immune receptor NKG2D, contains a conserved upstream promoter that expresses a noncoding transcript. Transcription from the upstream promoter represses the downstream standard promoter activity in cis through transcriptional interference. The effect of transcriptional interference depends on the strength of transcription from the upstream promoter and can be described quantitatively by a simple reciprocal repressor function...
April 11, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29627624/cryopreservation-and-egg-yolk-medium-alter-the-proteome-of-ram-spermatozoa
#15
T Pini, J P Rickard, T Leahy, B Crossett, X Druart, S P de Graaf
Cryopreservation causes significant lethal and sub-lethal damage to spermatozoa. In order to improve freezing outcomes, a comprehensive understanding of sub-lethal damage is required. Cryopreservation induced changes to sperm proteins have been investigated in several species, but few have employed currently available state of the art, data independent acquisition mass spectrometry (MS) methods. We used the SWATH LC-MS method to quantitatively profile proteomic changes to ram spermatozoa following exposure to egg yolk and cryopreservation...
April 5, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29627365/evolutionary-conservation-of-a-core-fungal-phosphate-homeostasis-pathway-coupled-to-development-in-blastocladiella-emersonii
#16
André L Gomes-Vieira, Jeremy G Wideman, Lisvane Paes-Vieira, Suely L Gomes, Thomas A Richards, José R Meyer-Fernandes
The model yeast Saccharomyces cerevisiae elicits a transcriptional response to phosphate (Pi ) depletion. To determine the origins of the phosphate response (PHO) system, we bioinformatically identified putative PHO components in the predicted proteomes of diverse fungi. Our results suggest that the PHO system is ancient; however, components have been expanded or lost in different fungal lineages. To show that a similar physiological response is present in deeply-diverging fungi we examined the transcriptional and physiological response of PHO genes to Pi depletion in the blastocladiomycete Blastocladiella emersonii...
April 5, 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29626545/hypoxia-selective-allosteric-destabilization-of-activin-receptor-like-kinases-a-potential-therapeutic-avenue-for-prophylaxis-of-heterotopic-ossification
#17
Guorong Lu, Mary R Tandang-Silvas, Alyssa C Dawson, Trenton J Dawson, Jay C Groppe
Heterotopic ossification (HO), the pathological extraskeletal formation of bone, can arise from blast injuries, severe burns, orthopedic procedures and gain-of-function mutations in a component of the bone morphogenetic protein (BMP) signaling pathway, the ACVR1/ALK2 receptor serine-threonine (protein) kinase, causative of Fibrodysplasia Ossificans Progressiva (FOP). All three ALKs (-2, -3, -6) that play roles in bone morphogenesis contribute to trauma-induced HO, hence are well-validated pharmacological targets...
April 4, 2018: Bone
https://www.readbyqxmd.com/read/29626480/tspa-as-a-novel-atf6%C3%AE-translocation-inducer-efficiently-ameliorates-insulin-sensitivity-restoration-and-glucose-homeostasis-in-db-db-mice
#18
Tingting Zhou, Yanhua Cheng, Wenzhong Yan, Xiaofan Shi, Xin Xu, Jinpei Zhou, Jian Li, Jing Chen, Xu Shen
Activating transcription factor 6α (ATF6α) as a transducer in unfolded protein response (UPR), plays an important role in liver glucose metabolism and insulin resistance. Thus, targeting ATF6α activation has been proposed to be a potential strategy for anti-T2DM drug discovery. Here, we determined that small molecule 2-[5-[1-(4-chlorophenoxy)ethyl]-4-phenyl-4H-1,2,4-triazol-3-yl]sulfanyl-N-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)acetamide (TSPA) functioned as an ATF6α translocation inducer effectively promoting ATF6α translocation into nucleus and ameliorating glucose homeostasis on db/db mice...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29626379/patterns-of-thaumarchaeal-gene-expression-in-culture-and-diverse-marine-environments
#19
Paul Carini, Christopher L Dupont, Alyson E Santoro
Thaumarchaea are ubiquitous in marine habitats where they participate in carbon and nitrogen cycling. Although metatranscriptomes suggest thaumarchaea are active microbes in marine waters, we understand little about how thaumarchaeal gene expression patterns relate to substrate utilization and activity. Here, we report the global transcriptional response of the marine ammonia-oxidizing thaumarchaeon 'Candidatus Nitrosopelagicus brevis' str. CN25 to ammonia limitation using RNA-Seq. We further describe the genome and transcriptome of Ca...
April 6, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29626215/the-coming-of-age-of-chaperone-mediated-autophagy
#20
REVIEW
Susmita Kaushik, Ana Maria Cuervo
Chaperone-mediated autophagy (CMA) was the first studied process that indicated that degradation of intracellular components by the lysosome can be selective - a concept that is now well accepted for other forms of autophagy. Lysosomes can degrade cellular cytosol in a nonspecific manner but can also discriminate what to target for degradation with the involvement of a degradation tag, a chaperone and a sophisticated mechanism to make the selected proteins cross the lysosomal membrane through a dedicated translocation complex...
April 6, 2018: Nature Reviews. Molecular Cell Biology
keyword
keyword
33199
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"