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https://www.readbyqxmd.com/read/29352712/evaluating-new-bio-hydrogen-producers-clostridium-perfringens-strain-jjc-clostridium-bifermentans-strain-wym-and-clostridium-sp-strain-ade-ty
#1
Yee Meng Wong, Ta Yeong Wu, Tau Chuan Ling, Pau Loke Show, Sze Ying Lee, Jo-Shu Chang, Shaliza Ibrahim, Joon Ching Juan
Three newly discovered H2 producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H2 production. The maximum H2 production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H2/mol glucose, respectively...
January 15, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29352709/analysis-of-gut-microbiota-diversity-and-auxiliary-diagnosis-as-a-biomarker-in-patients-with-schizophrenia-a-cross-sectional-study
#2
Yang Shen, Jintian Xu, Zhiyong Li, Yichen Huang, Ye Yuan, Jixiang Wang, Meng Zhang, Songnian Hu, Ying Liang
With the advent of sequencing technology, characterization of schizophrenia with underlying probing of gut microbiome can provide abundant clues for diagnosis and prognosis of schizophrenia. In this study, we first compared the difference of gut microbiota between schizophrenia patients and healthy controls by 16S rRNA sequencing. We further explored whether gut microbiota can be used as a biomarker to assist in the diagnosis of schizophrenia. We restricted inclusion criteria strictly to control confounding bias...
January 15, 2018: Schizophrenia Research
https://www.readbyqxmd.com/read/29352082/clostridial-strain-specific-characteristics-associated-with-necrotizing-enterocolitis
#3
Sophia Schönherr-Hellec, Geraldine L Klein, Johanne Delannoy, Laurent Ferraris, Jean Christophe Rozé, Marie José Butel, Julio Aires
We aimed at identifying potential clostridial factors linking these bacteria with necrotizing enterocolitis (NEC). We compared the phenotypic traits, stress responses, cellular cytotoxicity and inflammatory capabilities of the largest collection of Clostridium butyricum and Clostridium neonatale strains isolated from fecal samples of NEC and control preterm neonates (PN). When strains characteristics were used as explanatory variables, a statistical discriminant analysis allowed the separation of NEC and control strains into separate groups...
January 19, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29349925/ethanolamine-is-a-valuable-nutrient-source-that-impacts-clostridium-difficile-pathogenesis
#4
Kathryn L Nawrocki, Daniela Wetzel, Joshua B Jones, Emily C Woods, Shonna M McBride
Clostridium (Clostridioides) difficile is a gastrointestinal pathogen that colonizes the intestinal tract of mammals and can cause severe diarrheal disease. Although C. difficile growth is confined to the intestinal tract, our understanding of the specific metabolites and host factors that are important for the growth of the bacterium is limited. In other enteric pathogens, the membrane-derived metabolite, ethanolamine (EA), is utilized as a nutrient source and can function as a signal to initiate the production of virulence factors...
January 19, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29349869/clostridium-difficile-infection-is-associated-with-graft-loss-in-solid-organ-transplant-recipients
#5
A Cusini, C Béguelin, S Stampf, K Boggian, C Garzoni, M Koller, O Manuel, P Meylan, N J Mueller, H Hirsch, M Weisser, C Berger, C van Delden
Clostridium difficile infection (CDI) is a leading cause of infectious diarrhea in solid organ transplant recipients (SOT). We aimed to assess incidence, risk factors and outcome of CDI within the Swiss Transplant Cohort Study (STCS). We performed a case-control study of SOT recipients in the STCS diagnosed with CDI between May 2008 and August 2013. We matched two control subjects per case by age at transplantation, sex and transplanted organ. A multivariable analysis was performed using conditional logistic regression to identify risk factors and evaluate outcome of CDI...
January 19, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29349705/stool-cultures-at-the-icu-get-rid-of-it
#6
Carolin F Manthey, Darja Dranova, Martin Christner, Laura Berneking, Stefan Kluge, Ansgar W Lohse, Valentin Fuhrmann
BACKGROUND: Stool cultures for Campylobacter, Salmonella and Shigella and/or Yersinia spp. are frequently ordered in critically ill patients with diarrhea. The aim of this study is to analyze the diagnostic yield in a large cohort of critically ill patients. Therefore, we performed a cohort study at the Department of Intensive Care Medicine of a University Hospital (11 ICUs). RESULTS: From all patients who were admitted to the ICU between 2010 and 2015, stool cultures were taken from 2...
January 18, 2018: Annals of Intensive Care
https://www.readbyqxmd.com/read/29349441/association-between-sn-2-fatty-acid-profiles-of-breast-milk-and-development-of-the-infant-intestinal-microbiome
#7
Tiemin Jiang, Bin Liu, Jufang Li, Xueyan Dong, Man Lin, Minghui Zhang, Junying Zhao, Yaohua Dai, Lijun Chen
Increasing evidence shows that host diet and gut microbes are related. Previous studies have shown the effects of specific dietary fatty acids (FAs) on intestinal microbiota, but little is known about the effect of the stereospecifically numbered sn-2 position in triglycerides (TG) of human milk on the gut microbiome of infants. This study aimed at examining possible effects of sn-2 FAs of human milk on the gut microbial development of breastfeeding babies. Sn-2 FAs and intestinal microbiota were assessed by GC-MS and high-throughput 16S rRNA sequencing, respectively...
January 19, 2018: Food & Function
https://www.readbyqxmd.com/read/29348922/taxonogenomic-description-of-four-new-clostridium-species-isolated-from-human-gut-clostridium-amazonitimonense-clostridium-merdae-clostridium-massilidielmoense-and-clostridium-nigeriense
#8
M T Alou, S Ndongo, L Frégère, N Labas, C Andrieu, M Richez, C Couderc, J-P Baudoin, J Abrahão, S Brah, A Diallo, C Sokhna, N Cassir, B La Scola, F Cadoret, D Raoult
Culturomics investigates microbial diversity of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene identification. The present study allowed identification of four putative new Clostridium sensu stricto species: 'Clostridium amazonitimonense' strain LF2T, 'Clostridium massilidielmoense' strain MT26T, 'Clostridium nigeriense' strain Marseille-P2414T and 'Clostridium merdae' strain Marseille-P2953T, which we describe using the concept of taxonogenomics...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29348063/production-of-acids-and-alcohols-from-syngas-in-a-two-stage-continuous-fermentation-process
#9
Haris Nalakath Abubackar, María C Veiga, Christian Kennes
A two-stage continuous system with two stirred tank reactors in series was utilized to perform syngas fermentation using Clostridium carboxidivorans. The first bioreactor (bioreactor 1) was maintained at pH 6 to promote acidogenesis and the second one (bioreactor 2) at pH 5 to stimulate solventogenesis. Both reactors were operated in continuous mode by feeding syngas (CO:CO2:H2:N2; 30:10:20:40; vol%) at a constant flow rate while supplying a nutrient medium at different flow rates of 8.1, 15, 22 and 30 ml/h...
January 6, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29346543/the-impact-of-reported-beta-lactam-allergy-in-hospitalized-patients-with-hematologic-malignancies-requiring-antibiotics
#10
Kuan-Hsiang Gary Huang, Valerie Cluzet, Keith Hamilton, Olajumoke Fadugba
Background: Patients hospitalized with hematologic malignancy are particularly vulnerable to infection. The impact of reported beta-lactam (BL) allergy in this population remains unknown. Objective: To define the impact of reported BL-only allergy (BLOA) label on clinical outcomes compared to those with no BL allergy (NBLA) in hematologic malignancy inpatients requiring systemic antibiotics. Methods: Retrospective cohort study of adult inpatients with hematologic malignancy admitted at two tertiary care hospitals 2010-2015...
January 16, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29344709/microbial-characterization-of-methanogenic-and-iron-reducing-consortium-in-reactors-with-polychlorinated-biphenyls
#11
Mara R de Lima E Silva, Regiane C Correa, Isabel K Sakamoto, Maria B A Varesche
Recent papers have confirmed current environmental pollution and the continuous release of polychlorinated biphenyls (PCBs) despite the prohibition of its manufacture worldwide. As the dehalogenating microorganisms are able to remove halogens from various analogous compounds, the characterization of PCB metabolisms can improve the degradation of similar compounds. Thus, this study extensively evaluated the microbial community developed in methanogenic and iron-reducing reactors. The horizontal-flow anaerobic reactor (HAIB) with real waste of Aroclor (1 mL L-1) was fed with mineral medium, ethanol, and sodium formate...
January 17, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29344695/succession-of-microbial-communities-and-changes-of-incremental-oil-in-a-post-polymer-flooded-reservoir-with-nutrient-stimulation
#12
Peike Gao, Guoqiang Li, Jianjun Le, Xiaobo Liu, Fang Liu, Ting Ma
Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challengeable. In this study, indigenous microbial enhanced oil recovery (IMEOR) in a post-polymer flooded reservoir was performed. The succession of microbial communities was revealed by high-throughput sequencing of 16S rRNA genes and changes of incremental oil were analyzed. The results indicated that the abundances of reservoir microorganisms significantly increased, with alpha diversities decreased in the IMEOR process...
January 17, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29344087/determination-of-the-native-features-of-the-exoglucanase-cel48s-from-clostridium-thermocellum
#13
Ya-Jun Liu, Shiyue Liu, Sheng Dong, Renmin Li, Yingang Feng, Qiu Cui
Background: Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles and influences the activity and features of cellulosome to a great extent. Thus, it is of great importance to reveal the enzymatic features of Cel48S. However, Cel48S has not been well performed due to difficulties in purifying either recombinant or native Cel48S proteins...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29342372/enrichment-of-antibiotic-resistance-genes-in-soil-receiving-composts-derived-from-swine-manure-yard-wastes-or-food-wastes-and-evidence-for-multi-year-persistence-of-swine-i-i-clostridium-i-i-spp
#14
Andrew Scott, Yuan-Ching Tien, Craig F Drury, W Daniel Reynolds, Edward Topp
The impact of amendment with swine manure composts (SMC), yard waste compost (YWC), or food waste compost (FWC) on the abundance of antibiotic resistance genes in soil was evaluated. Following a commercial-scale application of the composts in a field experiment, soils were sampled periodically for a decade, and archived air-dried. Soil DNA was extracted and gene targets quantified by qPCR. Compared to untreated control soil, all three amendment types increased the abundance of gene targets for up to four years post-application...
January 17, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29342270/whole-genome-analysis-reveals-the-evolution-and-transmission-of-an-mdr-dh-nap11-106-clostridium-difficile-clone-in-a-paediatric-hospital
#15
Larry K Kociolek, Egon A Ozer, Dale N Gerding, David W Hecht, Sameer J Patel, Alan R Hauser
Background: Clostridium difficile strain DH/NAP11/106, a relatively antibiotic-susceptible strain, is now the most common cause of C. difficile infection (CDI) among adults in the USA. Objectives: To identify mechanisms underlying the evolution and transmission of an MDR DH/NAP11/106 clone. Methods: WGS (Illumina MiSeq), restriction endonuclease analysis (REA) and antibiotic susceptibility testing were performed on 134 C. difficile isolates collected from paediatric patients with CDI over a 2 year period...
January 12, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29339954/highlights-in-clostridium-difficile-infection-from-the-world-congress-of-gastroenterology-at-acg-2017-october-13-18-2017-%C3%A2-orlando-florida
#16
(no author information available yet)
No abstract text is available yet for this article.
December 2017: Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29339424/clostridium-sordellii-pathogenicity-locus-plasmid-pcs1-1-encodes-a-novel-clostridial-conjugation-locus
#17
Callum J Vidor, Thomas D Watts, Vicki Adams, Dieter Bulach, Edward Couchman, Julian I Rood, Neil F Fairweather, Milena Awad, Dena Lyras
A major virulence factor in Clostridium sordellii-mediated infection is the toxin TcsL, which is encoded within a region of the genome called the pathogenicity locus (PaLoc). C. sordellii isolates carry the PaLoc on the pCS1 family of plasmids, of which there are four characterized members. Here, we determined the potential mobility of pCS1 plasmids and characterized a fifth unique pCS1 member. Using a derivative of the pCS1-1 plasmid from strain ATCC 9714 which had been marked with the ermB erythromycin resistance gene, conjugative transfer into a recipient C...
January 16, 2018: MBio
https://www.readbyqxmd.com/read/29337612/impact-of-controlling-bacteria-in-feed-on-broiler-performance-during-a-clostridial-challenge
#18
K Richardson, C Hofacre, G Mathis, B Lumpkins, R Phillips
Three studies were conducted using Clostridium perfringens as an intestinal challenge to produce necrotic enteritis (NE). The studies consisted of two battery screening studies and one production study in floor pens. The purpose of the trials was to determine if reducing the level of microorganisms in feed consumed by broilers reduced the impact of a nonfeed-based Clostridial challenge. In all studies, C. perfringens challenged broilers consuming feed containing lower levels of microorganisms compared to control feed exhibited significantly ( P < 0...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29337611/interactions-between-parasites-and-the-bacterial-microbiota-of-chickens
#19
Rüdiger Hauck
Except for the important role coccidia have as predisposing factors of necrotic enteritis, the role parasites play in the dynamics of a healthy microbiota of chickens is not well explored. This review describes the interactions of relevant intestinal parasites of chickens with bacteria. Infection with Eimeria spp. favor the growth of Clostridium perfringens and suppress the growth of many other bacteria by increasing viscosity and passage time of the ingesta, and by causing lesions to the intestinal mucosa that improve the availability of nutrients for C...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29337344/microbiological-quality-and-safety-of-fresh-fruits-and-vegetables-at-retail-levels-in-korea
#20
Charles Nkufi Tango, Shuai Wei, Imran Khan, Mohammad Shakhawat Hussain, Paul-François Ngnitcho Kounkeu, Joong-Hyun Park, Se-Hun Kim, Deog Hwan Oh
The aim of this study was to evaluate the microbiological quality and safety of fresh produce at retail level in Korea in order to periodically update information and establish available risks associated with consumption of fresh fruits and vegetables. The samples from different markets located in 3 provinces of South Korea were collected. The protocol in the Korean Food Standards Codex was applied and generic Escherichia coli, coliforms, aerobic mesophilic bacteria (AMB), and yeast and mold (YM) in 360 packaged and unpackaged fresh fruits and vegetables were analyzed...
January 16, 2018: Journal of Food Science
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