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https://www.readbyqxmd.com/read/28328997/influence-of-intraocular-lens-subsurface-nanoglistenings-on-functional-visual-acuity
#1
Takahiro Hiraoka, Kazunori Miyata, Takeshi Hayashidera, Masaharu Iida, Keita Takada, Keiichiro Minami, Tetsuro Oshika
To investigate the influence of intraocular lens subsurface nanoglistenings (SSNGs) on functional visual acuity (FVA), thirty-nine eyes of 29 patients were examined in this study. The SSNG group comprised 19 eyes of 14 patients (75.7± 5.4 years, mean ± standard deviation), and the control group comprised 20 eyes of 15 patients (73.6 ± 6.5 years). The SSNGs were diagnosed on the basis of the typical whitish IOL appearance upon slit-lamp examination and results of densitometry regarding surface light scattering using Scheimpflug images...
2017: PloS One
https://www.readbyqxmd.com/read/28328621/ocular-involvement-in-children-with-inflammatory-bowel-disease
#2
Samuele Naviglio, Fulvio Parentin, Silvia Nider, Nicolò Rassu, Stefano Martelossi, Alessandro Ventura
BACKGROUND: Data on ocular manifestations of inflammatory bowel disease (IBD) in children are limited. Some authors have reported a high prevalence of asymptomatic uveitis, yet the significance of these observations is unknown and there are no recommendations on which ophthalmologic follow-up should be offered. METHODS: Children with IBD seen at a single referral center for pediatric gastroenterology were offered ophthalmologic evaluation as part of routine care for their disease...
March 21, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28324858/dual-lanthanide-doped-complexes-the-development-of-a-time-resolved-ratiometric-fluorescent-probe-for-anthrax-biomarker-and-a-paper-based-visual-sensor
#3
Qi-Xian Wang, Shi-Fan Xue, Zi-Han Chen, Shi-Hui Ma, Shengqiang Zhang, Guoyue Shi, Min Zhang
In this work, a novel time-resolved ratiometric fluorescent probe based on dual lanthanide (Tb: terbium, and Eu: europium)-doped complexes (Tb/DPA@SiO2-Eu/GMP) has been designed for detecting anthrax biomarker (dipicolinic acid, DPA), a unique and major component of anthrax spores. In such complexes-based probe, Tb/DPA@SiO2 can serve as a stable reference signal with green fluorescence and Eu/GMP act as a sensitive response signal with red fluorescence for ratiometric fluorescent sensing DPA. Additionally, the probe exhibits long fluorescence lifetime, which can significantly reduce the autofluorescence interferences from biological samples by using time-resolved fluorescence measurement...
March 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28324245/comparison-of-dna-microarray-loop-mediated-isothermal-amplification-lamp-and-real-time-pcr-with-dna-sequencing-for-identification-of-fusarium-spp-obtained-from-patients-with-hematologic-malignancies
#4
Marcela de Souza, Tetsuhiro Matsuzawa, Kanae Sakai, Yasunori Muraosa, Luzia Lyra, Ariane Fidelis Busso-Lopes, Anna Sara Shafferman Levin, Angélica Zaninelli Schreiber, Yuzuru Mikami, Tohoru Gonoi, Katsuhiko Kamei, Maria Luiza Moretti, Plínio Trabasso
The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%...
March 21, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28324243/fungal-keratitis-secondary-to-trametes-betulina-a-case-report-and-review-of-literature
#5
Joshua S Hardin, Deanna A Sutton, Nathan P Wiederhold, James Mele, Sunali Goyal
PURPOSE: We report the first case of human infection and keratitis secondary to Trametes betulina, a rare filamentous fungus. METHODS: Clinical examination including external and slit-lamp examination and corneal scrapings with microbiologic evaluation were performed on a patient with chronic allergic conjunctivitis, entropion and a long-standing corneal ulcer resistant to treatment. RESULTS: The culture from the corneal scraping revealed a basidiomycetous fungus which was submitted for identification...
March 21, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28323482/constraints-on-the-early-terrestrial-surface-uv-environment-relevant-to-prebiotic-chemistry
#6
Sukrit Ranjan, Dimitar D Sasselov
The UV environment is a key boundary condition to abiogenesis. However, considerable uncertainty exists as to planetary conditions and hence surface UV at abiogenesis. Here, we present two-stream multilayer clear-sky calculations of the UV surface radiance on Earth at 3.9 Ga to constrain the UV surface fluence as a function of albedo, solar zenith angle (SZA), and atmospheric composition. Variation in albedo and latitude (through SZA) can affect maximum photoreaction rates by a factor of >10.4; for the same atmosphere, photoreactions can proceed an order of magnitude faster at the equator of a snowball Earth than at the poles of a warmer world...
March 2017: Astrobiology
https://www.readbyqxmd.com/read/28323152/loop-mediated-isothermal-amplification-lamp-a-novel-rapid-detection-platform-for-pathogens
#7
REVIEW
Yanmei Li, Penghui Fan, Shishui Zhou, Li Zhang
Foodborne bacterial infections and diseases have been considered to be a major threat for public health in the worldwide. Increased incidence of human diseases caused by foodborne pathogens have been correlated with growing world population and mobility. Loop-mediated isothermal amplification (LAMP) has been regarded as an innovative gene amplification technology and emerged as an alternative to PCR-based methodologies in both clinical laboratory and food safety testing. Nowadays, LAMP has been applied to detection and identification on pathogens from microbial diseases, as it showed significant advantage in high sensitivity, specificity and rapidity...
March 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28322309/multiplex-reverse-transcription-loop-mediated-isothermal-amplification-coupled-with-cascade-invasive-reaction-and-nanoparticle-hybridization-for-subtyping-of-influenza-a-virus
#8
Ying Chi, Yiyue Ge, Kangchen Zhao, Bingjie Zou, Bin Liu, Xian Qi, Qian Bian, Zhiyang Shi, Fengcai Zhu, Minghao Zhou, Lunbiao Cui, Chuan Su
Considering the fatal human victims and economic loss caused by influenza virus infection every year, methodologies for rapid and on-site detection of influenza viruses are urgently needed. LAMP is the most commonly used nucleic acid isothermal amplification technology suitable for on-site use. However, for multiplex LAMP, differentiation of the amplicons derived from multiple targets is still challengeable currently. Here we developed a multiplex RT-LAMP assay for simultaneous amplification of three prominent subtypes of influenza viruses (A/H5, A/H7 and 2009A/H1)...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28321260/loop-mediated-isothermal-amplification-test-for-trypanosoma-gambiense-group-1-with-stem-primers-a-molecular-xenomonitoring-test-for-sleeping-sickness
#9
Zablon K Njiru, Cecilia K Mbae, Gitonga N Mburugu
The World Health Organization has targeted Human African Trypanosomiasis (HAT) for elimination by 2020 with zero incidence by 2030. To achieve and sustain this goal, accurate and easy-to-deploy diagnostic tests for Gambian trypanosomiasis which accounts for over 98% of reported cases will play a crucial role. Most needed will be tools for surveillance of pathogen in vectors (xenomonitoring) since population screening tests are readily available. The development of new tests is expensive and takes a long time while incremental improvement of existing technologies that have potential for xenomonitoring may offer a shorter pathway to tools for HAT surveillance...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28319246/estimated-perinatal-hiv-infection-among-infants-born-in-the-united-states-2002-2013
#10
Allan W Taylor, Steven R Nesheim, Xinjian Zhang, Ruiguang Song, Lauren F FitzHarris, Margaret A Lampe, Paul J Weidle, Patricia Sweeney
Importance: Perinatal transmission of human immunodeficiency virus (HIV) can be reduced through services including antiretroviral treatment and prophylaxis. Data on the national incidence of perinatal HIV transmission and missed prevention opportunities are needed to monitor progress toward elimination of mother-to-child HIV transmission. Objective: To estimate the number of perinatal HIV cases among infants born in the United States. Design, Setting, and Participants: Data were obtained from the National HIV Surveillance System on infants with HIV born in the United States (including the District of Columbia) and their mothers between 2002 and 2013 (reported through December 31, 2015)...
March 20, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28318635/the-application-of-infrared-imaging-and-optical-coherence-tomography-of-the-lacrimal-punctum-in-patients-undergoing-punctoplasty-for-epiphora
#11
Hannah M Timlin, Pearse A Keane, Geoffrey E Rose, Daniel G Ezra
PURPOSE: To determine the application of imaging the stenotic lacrimal punctum with infrared photographs and optical coherence tomography (OCT) and to identify characteristics of the lacrimal punctum in patients who benefit from punctoplasty. DESIGN: Case-control study. PARTICIPANTS: Twenty patients with epiphora who were listed for punctoplasty and 20 healthy controls. METHODS: Prospectively, 20 patients listed for punctoplasty were asked to rate their epiphora, using the Munk score, before and after punctoplasty...
March 16, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28318383/detection-of-brucellosis-in-sika-deer-cervus-nippon-through-loop-mediated-isothermal-amplification-lamp
#12
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, Wenrong Wu
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis (Brucella bovis) in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1...
March 20, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28318342/pattern-of-uveitic-hypotony-in-a-tertiary-eye-hospital-in-india
#13
Parthopratim Dutta Majumder, Keerthi Burugupalli, Nitin Kumar Menia, Sudha K Ganesh, Jyotirmay Biswas
PURPOSE: To report pattern of uveitic patients with hypotony in Indian population. METHODS: Retrospective medical record review of 30 eyes of 21 consecutive patients with uveitic hypotony. Patient demographics, ocular findings and systemic association were recorded. RESULTS: Mean age of the patients was 29.5 years (range 7-71 years) and 80.9%(17 patients) were below the age of 40 years. Most common complaint associated with the development of hypotony was diminution of vision (26 eyes; 86...
March 20, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28317856/a-smartphone-based-diagnostic-platform-for-rapid-detection-of-zika-chikungunya-and-dengue-viruses
#14
Aashish Priye, Sara W Bird, Yooli K Light, Cameron S Ball, Oscar A Negrete, Robert J Meagher
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316195/-analysis-of-corneal-complications-in-children-wearing-orthokeratology-lenses-at-night
#15
C X Miao, X Y Xu, H Zhang
Objective: To explore the characteristics, prognoses and corneal complications of children wearing orthokeratology lenses (Ortho-K) at night. Methods: Retrospective case analysis. A total of 113 cases (219 eyes) wore Ortho-K for myopia at night, including 7 cases with unilateral glass wear. The average age was (11.5±2.0) years old. The myopia ranged from -1.00 D to -9.00 D in all eyes and from -2.00 D to -4.00 D in 157 of them. Examinations were performed before lens wearing, at 1 hour, the next morning, 2 days, 3 days, 1 week and 2 weeks after lens wearing, and thereafter once a month...
March 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28316191/-in-vivo-confocal-microscopic-observation-of-corneal-changes-in-patients-with-chronic-stevens-johnson-syndrome-or-toxic-epidermal-necrolysis
#16
T T Gao, Y Li, J Liu, Q Long
Objective: To describe corneal alterations visible on in vivo confocal microscopy in patients with debilitating ocular sequelae caused by Stevens-Johnson syndrome (SJS) or toxic epidermal necrolysis (TEN). Methods: Twenty-two eyes of 11 consecutive patients suffering from chronic SJS or TEN were studied using slit lamp and in vivo confocal microscopy in the Ophthalmology Outpatient Department of Peking Union Medical College Hospital from March 2014 to April 2015. Results: Dry eye with meibomian gland dysfunction (19 eyes of 10 patients, 86...
March 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28306716/localization-and-diagnosis-framework-for-pediatric-cataracts-based-on-slit-lamp-images-using-deep-features-of-a-convolutional-neural-network
#17
Xiyang Liu, Jiewei Jiang, Kai Zhang, Erping Long, Jiangtao Cui, Mingmin Zhu, Yingying An, Jia Zhang, Zhenzhen Liu, Zhuoling Lin, Xiaoyan Li, Jingjing Chen, Qianzhong Cao, Jing Li, Xiaohang Wu, Dongni Wang, Haotian Lin
Slit-lamp images play an essential role for diagnosis of pediatric cataracts. We present a computer vision-based framework for the automatic localization and diagnosis of slit-lamp images by identifying the lens region of interest (ROI) and employing a deep learning convolutional neural network (CNN). First, three grading degrees for slit-lamp images are proposed in conjunction with three leading ophthalmologists. The lens ROI is located in an automated manner in the original image using two successive applications of Candy detection and the Hough transform, which are cropped, resized to a fixed size and used to form pediatric cataract datasets...
2017: PloS One
https://www.readbyqxmd.com/read/28304013/loop-mediated-isothermal-amplification-tb-lamp-a-step-forward-in-the-diagnosis-of-tuberculosis
#18
Hanieya Saiyed, Sarosh Fatima
No abstract text is available yet for this article.
March 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28303825/evaluation-of-loop-mediated-isothermal-amplification-assay-for-rapid-diagnosis-of-acanthamoeba-keratitis
#19
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
BACKGROUND: The clinical features of Acanthamoeba keratitis (AK) are non-specific and closely resemble bacterial, viral and fungal keratitis. MATERIALS AND METHODS: We compared loop-mediated isothermal amplification (LAMP) with microscopy, non-nutrient agar (NNA) culture and polymerase chain reaction (PCR) in clinical suspects of AK. RESULTS: Of 52 clinical samples (42 AK suspects and 10 proven bacterial, viral or fungal keratitis), 3 were positive by direct microscopy (sensitivity 60%, confidence interval [CI]: 17%-92...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28302249/opacification-of-a-hydrophilic-acrylic-intraocular-lens
#20
Yezhen Yang, Manqiang Peng, Yiqin Duan, Xuetao Huang, Kuanshu Li, Ding Lin
Opacification of a hydrophilic acrylic intraocular (IOL) lens is a rare phenomenon. We herein report a case of a 57-year man complaining of decreased vision at left eye for the last 4 months. He had undergone phacoemulsification with IOL implantation 2 years back. IOL opacification was observed through slit-lamp. IOL exchange was carried out. The exchanged IOL and a new lens of the same model were sent to laboratory for pathologic analysis. Confocal microscopy showed uniformly distributed granules from the surface to 80 µm internal surface...
March 2017: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
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