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https://www.readbyqxmd.com/read/28735236/oxidation-of-tris-2-chloroethyl-phosphate-in-aqueous-solution-by-uv-activated-peroxymonosulfate-kinetics-water-matrix-effects-degradation-products-and-reaction-pathways
#1
Xinxin Xu, Jing Chen, Ruijuan Qu, Zunyao Wang
The feasibility of UV-activated peroxymonosulfate (PMS) technology for the degradation of Tris (2-chloroethyl) phosphate (TCEP) in an aqueous solution was investigated in this study. The conditions of [PMS]0: [TCEP]0 = 20:1, T = 25 ± 2 °C and pH = 5.5 ± 0.5 cause a 94.6% removal of TCEP (1 mg L(-1)) after 30 min of Hg lamp irradiation. The effects of operating parameters (the oxidant doses, pH and presence of typical cations (Fe(3+), Cu(2+), Ni(2+), NH4(+)), anions (Cl(-), HCO3(-), NO3(-), HPO4(2-)) and humic acid (HA)) were evaluated...
July 19, 2017: Chemosphere
https://www.readbyqxmd.com/read/28731934/long-term-evaluation-of-specular-microscopic-changes-following-nd-yag-iridotomy-in-chronic-primary-angle-closure-glaucoma-eyes
#2
Ramanjit Sihota, Esha Agarwal, Mathew James, Meena Verma, Lalit Kumar, Tanuj Dada, Viney Gupta, Kulwant Singh Kapoor
AIM: The aim of this study was to evaluate specular microscopy of chronic primary angle-closure glaucoma (CPACG) eyes at least 1 year after Nd:YAG iridotomy, and compare them with CPACG eyes without an iridotomy and age-matched, normal eyes. PATIENTS AND METHOD: Consecutive patients of CPACG at the Glaucoma service were screened. All patients underwent slit-lamp biomicroscopy, +90 D examination, and applanation tonometry. A total of 100 eyes of 100 consecutive patients of CPACG with an Nd:YAG iridotomy performed ≥1 year before, who met all inclusion/exclusion criteria, 60 consecutive CPACG eyes without an iridotomy, and 60 age and refraction-matched control eyes were enrolled...
July 20, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28731879/peripheral-endothelial-cell-count-is-a-predictor-of-disease-severity-in-advanced-fuchs-endothelial-corneal-dystrophy
#3
Zeba A Syed, Jennifer A Tran, Ula V Jurkunas
PURPOSE: In advanced Fuchs endothelial corneal dystrophy (FECD), central endothelial changes do not correlate with disease severity. The peripheral endothelial cell count (ECC) has not been studied as a marker of FECD severity. The goal of this study was to determine the relationship between the peripheral ECC and known clinical markers of FECD in advanced cases. METHODS: Patients with FECD examined between January 1, 2013, and September 1, 2016, by 1 cornea specialist were identified...
July 19, 2017: Cornea
https://www.readbyqxmd.com/read/28731284/the-rna-binding-protein-hur-inhibits-expression-of-ccl5-and-limits-recruitment-of-macrophages-into-tumors
#4
Thilo F Brauß, Sofia Winslow, Sebastian Lampe, Anica Scholz, Andreas Weigert, Nathalie Dehne, Kristoffer von Stedingk, Tobias Schmid, Bernhard Brüne
The RNA-binding protein HuR promotes tumor growth by affecting proliferation, metastasis, apoptosis, and angiogenesis. Although immune cells, especially tumor-associated macrophages, are critical components of the tumor stroma, the influence of HuR in tumors on the recruitment of immune cells remains poorly understood. In the present study, we therefore aimed to elucidate the impact of tumor cell HuR on the interaction between tumor cells and macrophages. To this end, we stably depleted HuR in human MCF-7 breast cancer cells...
July 21, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28730110/incidence-of-cystoid-macular-edema-after-descemet-s-stripping-automated-endothelial-keratoplasty
#5
Eduard Pedemonte-Sarrias, Toni Salvador Playà, Irene Sassot Cladera, Oscar Gris, Joan Ribas Martínez, José García-Arumí, Núria Giménez
AIM: To determine the incidence of cystoid macular edema (CME) after Descemet's stripping automated endothelial keratoplasty (DSAEK). METHODS: This study included all consecutive patients operated in a Spanish tertiary reference hospital over a period of four years. A total of 55 eyes from 47 patients matched the selection criteria. CME was diagnosed clinically at the slit-lamp and confirmed by optical coherence tomography. RESULTS: Six cases of CME were diagnosed postoperatively, which represented an incidence of 11%...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28730087/frequency-of-diabetic-retinopathy-and-associated-risk-factors-in-khartoum-sudan-population-based-study
#6
Einas S Elwali, Ahmed O Almobarak, Mona A Hassan, Amir A Mahmooud, Heitham Awadalla, Mohamed H Ahmed
AIM: To assess the frequency and associated risk factors of diabetic retinopathy among Sudanese individuals with diabetes attending Makka Eye complex in Khartoum, Sudan. METHODS: The cross sectional hospital based study recruited 316 individuals with diabetes from Makkah Eye Complex Retina Clinic. Standard questionnaire was used to collect demographic data, medical history and life style characteristics. Blood samples were taken to measure HbA1c and lipid profile...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28729556/observation-of-aggregation-triggered-by-resonance-energy-transfer-ret-induced-intermolecular-pairing-force
#7
Xiaoyong Pan, Weizhi Wang, Lin Ke, Nan Zhang
In this report, we showed the existence of RET induced intermolecular pairing force by comparing their fluorescence behaviors under room illumination vs standing in dark area for either PFluAnt solution or PFluAnt&PFOBT mixture. Their prominent emission attenuation under room illumination brought out the critical role of photo, i.e. RET induced intermolecular pairing force in induction of polymer aggregation. Constant UV-Vis absorption and fluorescence spectra in terms of both peak shapes and maximum wavelengths implied no chemical decomposition was involved...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728873/inactivation-of-alicyclobacillus-acidoterrestris-atcc-49025-spores-in-apple-juice-by-pulsed-light-influence-of-initial-contamination-and-required-reduction-levels
#8
Mariana I Ferrario, Sandra N Guerrero
The purpose of this study was to analyze the response of different initial contamination levels of Alicyclobacillus acidoterrestris ATCC 49025 spores in apple juice as affected by pulsed light treatment (PL, batch mode, xenon lamp, 3pulses/s, 0-71.6J/cm(2)). Biphasic and Weibull frequency distribution models were used to characterize the relationship between inoculum size and treatment time with the reductions achieved after PL exposure. Additionally, a second order polynomial model was computed to relate required PL processing time to inoculum size and requested log reductions...
July 17, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28728287/-analysis-of-structure-and-function-of-meibomian-glands-in-healthy-population-at-different-ages
#9
J Hong, H Q Qu
Objective: To observe the structure and function of meibomian glands in normal population at different ages. Methods: From October 2011 to August 2012, meibomian gland information was collected in healthy volunteers without any symptoms of ocular discomfort, aged more than 5 years. The people were grouped by every 10 years of age. The meibomian gland opening, secretion state and characteristics and Max's line were observed by slit lamp microscopic examination, and the meibomian gland dropouts were examined by noncontact infrared meibomian gland microscopy...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728285/-prevalence-and-surgery-status-of-cataract-among-adults-aged-60-years-or-above-in-two-villages-of-nantong
#10
J Zhou, Y Yuan, X Zhang, M Yang, H J Guan
Objective: To assess the prevalence, surgical coverage rate and social burden rate of cataract blindness among adults aged ≥60 years in the suburban population of Nantong, Jiangsu, China. Methods: It was a population-based cross-section study. A census with individuals aged ≥60 years was conducted in two villages of the suburb area in Nantong from June to August, 2008. A total of 1 305 individuals received visual acuity and eye examinations. Lens and cataract operative status were evaluated by slit-lamp biomicroscopy...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728284/-association-of-diabetic-retinopathy-with-diabetic-peripheral-neuropathy-in-type-2-diabetic-patients-the-beijing-desheng-diabetic-eye-disease-study
#11
W W Wei, X F Yang, H Gu, N P Liu
Objective: To determine the prevalence of diabetic retinopathy (DR) and diabetic peripheral neuropathy (DPN) in the Beijing Desheng Diabetic Eye Disease Study cohort and to investigate the association between DR and DPN. Methods: Cross-sectional study in a type 2 diabetic cohort. Questionnaires were collected and physical examinations were performed. Ophthalmologic examinations consisted of visual acuity with pinhole, slit-lamp examination of the anterior segment and seven-field 30(o) mydriatic fundus photography...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28727723/analytical-sensitivity-and-specificity-of-a-loop-mediated-isothermal-amplification-lamp-kit-prototype-for-detection-of-trypanosoma-cruzi-dna-in-human-blood-samples
#12
Susana A Besuschio, Mónica Llano Murcia, Alejandro F Benatar, Severine Monnerat, Israel Cruz Mata, Albert Picado de Puig, María de Los Ángeles Curto, Yutaka Kubota, Diana P Wehrendt, Paula Pavia, Yasuyoshi Mori, Concepción Puerta, Joseph M Ndung'u, Alejandro G Schijman
This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside of the caps. The reaction is carried out at 65°C during 40 minutes. Calcein allows direct detection of amplified products with the naked eye. Inclusivity and selectivity were tested in purified DNA from Trypanosoma cruzi stocks belonging to the six discrete typing units (DTUs), in DNA from other protozoan parasites and in human DNA...
July 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28727121/supplementing-newborn-intrauterine-growth-restricted-piglets-with-a-bolus-of-porcine-colostrum-raises-rectal-temperatures-one-degree-celsius
#13
C Amdi, L L Jensen, N Oksbjerg, C F Hansen
Hyperprolific sows have increased litter sizes but also result in more piglets that have been exposed to intrauterine growth restriction (IUGR). These IUGR piglets are likely to have a low rectal temperature and lower blood glucose levels compared with normal piglets at birth. Therefore, we hypothesized that a colostrum bolus at birth and/or heat from an external source would have a positive effect on blood glucose levels, rectal temperatures, and growth up to 8 h postpartum. In addition, liver glycogen and blood values at 8 h were investigated...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28720542/one-step-reverse-transcription-loop-mediated-isothermal-amplification-assay-for-detection-of-apple-chlorotic-leaf-spot-virus
#14
Dandan Peng, Jipeng Xie, Wei Qiang, Kai-Shu Ling, Liyun Guo, Zaifeng Fan, Tao Zhou
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Apple chlorotic leaf spot virus (ACLSV). In this method, a set of four primers was designed based on the conserved regions in the coat protein gene of ACLSV, and the primers were synthesized for the RT-LAMP assay using total RNA extracted from ACLSV-infected leaf tissues. The optimal reaction temperature and assay time were determined to be 64°C and 75min, respectively. The sensitivity of RT-LAMP reactions was reliable up to a maximum dilution of 1:3125, which was more sensitive than the RT-PCR assay...
July 15, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28719189/determination-of-double-bond-positions-in-polyunsaturated-fatty-acids-using-the-photochemical-patern%C3%A3-b%C3%A3-chi-reaction-with-acetone-and-tandem-mass-spectrometry
#15
Robert C Murphy, Toshiaki Okuno, Christopher A Johnson, Robert M Barkley
The position of double bonds along the carbon chain of methylene interrupted polyunsaturated fatty acids are unique identifiers of specific fatty acids derived from biochemical reactions that occur in cells. It is possible to obtain direct structural information as to these double bond positions using tandem mass spectrometry after collisional activation of the carboxylate anions of an acetone adduct at each of the double bond positions formed by the photochemical Paternò-Büchi reaction with acetone. This reaction can be carried out by exposing a small portion of an inline fused silica capillary to UV photons from a mercury vapor lamp as the sample is infused into the electrospray ion source of a mass spectrometer...
July 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28717386/understanding-low-radiation-background-biology-through-controlled-evolution-experiments
#16
Nathanael Lampe, Vincent Breton, David Sarramia, Télesphore Sime-Ngando, David G Biron
Biological experiments conducted in underground laboratories over the last decade have shown that life can respond to relatively small changes in the radiation background in unconventional ways. Rapid changes in cell growth, indicative of hormetic behaviour and long-term inheritable changes in antioxidant regulation have been observed in response to changes in the radiation background that should be almost undetectable to cells. Here, we summarize the recent body of underground experiments conducted to date, and outline potential mechanisms (such as cell signalling, DNA repair and antioxidant regulation) that could mediate the response of cells to low radiation backgrounds...
August 2017: Evolutionary Applications
https://www.readbyqxmd.com/read/28717256/ketone-catalyzed-photochemical-c-sp-3-h-chlorination
#17
Lei Han, Jibao Xia, Lin You, Chuo Chen
Photoexcited arylketones catalyze the direct chlorination of C(sp(3))-H groups by N-chlorosuccinimide. Acetophenone is the most effective catalyst for functionalization of unactivated C-H groups while benzophenone provides better yields for benzylic C-H functionalization. Activation of both acetophenone and benzophenone can be achieved by irradiation with a household compact fluorescent lamp. This light-dependent reaction provides a better control of the reaction as compared to the traditional chlorination methods that proceed through a free radical chain propagation mechanism...
June 29, 2017: Tetrahedron
https://www.readbyqxmd.com/read/28716373/loop-mediated-isothermal-amplification-in-disposable-polyester-toner-microdevices
#18
Kezia Gomes de Oliveira, Juliane Cristina Borba, Alexandre Melo Bailão, Célia Maria de Almeida Soares, Emanuel Carrilho, Gabriela Rodrigues Mendes Duarte
In recent years, isothermal DNA amplification methods have emerged as an alternative in molecular diagnostics due to its ease of operation. The purpose of using isothermal amplification is to simplify the diagnostics work by i) eliminating heating cycles, ii) reducing equipment costs, and iii) providing rapid and accurate results in laboratories with limited resources. Here we show a simple and fast method for E. coli detection in disposable polyester-toner (PeT) microdevice. The amplification by LAMP of the malB gene from E...
July 14, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28716289/devic-s-disease-before-devic-on-the-contribution-of-friedrich-albin-schanz-1863-1923
#19
S Jarius, B Wildemann
Neuromyelitis optica (NMO, Devic's disease) is an autoimmune disorder of the CNS that mainly affects the optic nerves and spinal cord and, if not treated, can leave patients blind and wheelchair-bound. NMO was long considered a clinical variant of multiple sclerosis (MS). While the history of MS has been studied extensively, relatively little is known about the early history of NMO. A dissertation written by Fernand Gault (1873-1936), a doctoral student to Eugène Devic (1858-1930), a Lyon-based neurologist after whom the disease would later be named, is considered by many the first review of NMO...
August 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28714924/cross-sectional-study-to-determine-the-prevalence-of-hepatitis-b-and-c-virus-infection-in-high-risk-groups-in-the-northeast-region-of-brazil
#20
Jakeline Ribeiro Barbosa, Cristianne Sousa Bezerra, Filipe Anibal Carvalho-Costa, Carolina Pimentel de Azevedo, Geane Lopes Flores, Jeová Keny Baima Colares, Danielle Malta Lima, Elisabeth Lampe, Lívia Melo Villar
Background: HBV (Hepatitis B Virus) and HCV (Hepatitis C Virus) infections are more prevalent in vulnerable populations than the general population. The objective of this study was to investigate the prevalence of HBV and HCV infection in HIV-positive patients (GI), chronic renal failure (CRF) patients (GII) and coagulation disorder individuals (GIII). Methods: A cross-sectional study was conducted from June 2014 to March 2015. Serum samples were tested for markers of hepatitis B and C by enzyme-linked immunosorbent assay (ELISA)...
July 17, 2017: International Journal of Environmental Research and Public Health
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