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https://www.readbyqxmd.com/read/28938418/androgens-support-male-acrobatic-courtship-behavior-by-enhancing-muscle-speed-and-easing-the-severity-of-its-trade-off-with-force
#1
Matthew J Fuxjager, Meredith C Miles, Franz Goller, John Petersen, Julia Yancey
Steroid hormone action in the brain regulates many animals' elaborate social displays used for courtship and competition, but it is increasingly recognized that the periphery may also be a site for potent steroidal modulation of complex behavior. However, the mechanisms of such "bottom up" regulation of behavioral outflow are largely unclear. To study this problem, we examine how androgenic sex hormones act through the skeletal muscular system to mediate elaborate courtship acrobatics in a tropical bird called the golden-collared manakin...
August 29, 2017: Endocrinology
https://www.readbyqxmd.com/read/28937648/separation-and-enrichment-of-lectin-from-zihua-snap-bean-phaseolus-vulgaris-seeds-by-peg-600-ammonium-sulfate-aqueous-two-phase-system
#2
Bin Jiang, Yongqiang Yuan, Xiaoqing Zhang, Zhibiao Feng, Chunhong Liu
A fast and efficient method based on a polyethylene glycol (PEG) 600/(NH₄)₂SO₄ aqueous two-phase system for extracting lectin from Zihua snap-bean (Phaseolus vulgaris) seeds was established. According to a Box-Behnken design (BBD), involving four factors at three levels each subjected to analysis of variance (ANOVA) and response surface analysis, the protein recovery and the purification factor of lectin in the top phase were used as the response values of the variance analysis to acquire the multivariate quadratic regression model...
September 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28935937/membrane-tension-increases-fusion-efficiency-of-model-membranes-in-the-presence-of-snares
#3
Torben-Tobias Kliesch, Jörn Dietz, Laura Turco, Partho Halder, Elena Polo, Marco Tarantola, Reinhard Jahn, Andreas Janshoff
The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934080/could-ebt-machines-increase-fruit-and-vegetable-purchases-at-new-york-city-green-carts
#4
Andrew Breck, Kamila Kiszko, Olivia Martinez, Courtney Abrams, Brian Elbel
INTRODUCTION: Residents of some low-income neighborhoods have limited access to fresh fruits and vegetables. In 2008, New York City issued new mobile fruit and vegetable cart licenses for neighborhoods with inadequate availability of fresh produce. Some of these carts were equipped with electronic benefit transfer (EBT) machines, allowing them to accept Supplemental Nutrition Assistance Program (SNAP) benefits. This article examines the association between type and quantities of fruits and vegetables purchased from mobile fruit and vegetable vendors and consumer characteristics, including payment method...
September 21, 2017: Preventing Chronic Disease
https://www.readbyqxmd.com/read/28933823/two-color-810-nm-sted-nanoscopy-of-living-cells-with-endogenous-snap-tagged-fusion-proteins
#5
Alexey N Butkevich, Haisen Ta, Michael Ratz, Stefan Stold, Stefan Jakobs, Vladimir N Belov, Stefan W Hell
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell superresolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP-tag, together with a non-covalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.
September 21, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28930488/the-impact-of-repeated-freeze-thaw-cycles-on-the-quality-of-biomolecules-in-four-different-tissues
#6
Xiaoli Ji, Min Wang, Lingling Li, Fang Chen, Yanyang Zhang, Qian Li, Junmei Zhou
High-quality biosamples are valuable resources for biomedical research. However, some tissues are stored without being sectioned into small aliquots and have to undergo repeated freeze-thaw cycles throughout prolonged experimentation. Little is known regarding the effects of repeated freeze-thaw cycles on the quality of biomolecules in tissues. The aim of this study was to evaluate the impact of repeated freeze-thaw (at room temperature or on ice) cycles on biomolecules and gene expression in four different types of tissues...
September 20, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28925945/measurement-of-respiratory-chain-enzyme-activity-in-human-renal-biopsy-specimens
#7
Arun Ghose, Christopher M Taylor, Alexander J Howie, Anapurna Chalasani, Iain Hargreaves, David V Milford
Background: Mitochondrial disorders can present as kidney disease in children and be difficult to diagnose. Measurement of mitochondrial function in kidney tissue may help diagnosis. This study was to assess the feasibility of obtaining renal samples and analysing them for respiratory chain enzyme activity. Methods: The subjects were children undergoing a routine diagnostic renal biopsy, in whom a clinical condition of renal inflammation, scarring and primary metabolic disorder was unlikely. A fresh sample of kidney was snap frozen and later assayed for the activities of respiratory chain enzyme complexes I, II/III, and IV using spectrophotometric enzyme assay, and expressed as a ratio of citrate synthase activity...
September 19, 2017: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/28925353/sec17-%C3%AE-snap-and-an-sm-tethering-complex-regulate%C3%A2-the-outcome-of-snare-zippering-in-vitro-and-in-vivo
#8
Matthew L Schwartz, Daniel P Nickerson, Braden T Lobingier, Rachael L Plemel, Mengtong Duan, Cortney G Angers, Michael Zick, Alexey J Merz
Zippering of SNARE complexes spanning docked membranes is essential for most intracellular fusion events. Here we explore how SNARE regulators operate on discrete zippering states. The formation of a metastable trans-complex, catalyzed by HOPS and its SM subunit Vps33, is followed by subsequent zippering transitions that increase the probability of fusion. Operating independently of Sec18 (NSF) catalysis, Sec17 (α-SNAP) either inhibits or stimulates SNARE-mediated fusion. If HOPS or Vps33 are absent, Sec17 inhibits fusion at an early stage...
September 19, 2017: ELife
https://www.readbyqxmd.com/read/28924668/synthesis-of-janelia-fluor-halotag-and-snap-tag-ligands-and-their-use-in-cellular-imaging-experiments
#9
Jonathan B Grimm, Timothy A Brown, Brian P English, Timothée Lionnet, Luke D Lavis
The development of genetically encoded self-labeling protein tags such as the HaloTag and SNAP-tag has expanded the utility of chemical dyes in microscopy. Intracellular labeling using these systems requires small, cell-permeable dyes with high brightness and photostability. We recently discovered a general method to improve the properties of classic fluorophores by replacing N,N-dimethylamino groups with four-membered azetidine rings to create the "Janelia Fluor" dyes. Here, we describe the synthesis of the HaloTag and SNAP-tag ligands of Janelia Fluor 549 and Janelia Fluor 646 as well as standard labeling protocols for use in ensemble and single-molecule cellular imaging...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28924664/two-color-single-molecule-tracking-in-live-cells
#10
Siegfried Hänselmann, Dirk-Peter Herten
Measuring the kinetics of protein-protein interactions between molecules in the plasma membrane of live cells provides valuable information for understanding dynamic processes, like cellular signaling, on a molecular scale. Two-color single-molecule tracking is a fluorescence microscopy-based method to detect and quantify specific protein-protein interactions on a single-event level, providing sensitivity to heterogeneities and rare events. Fundamentally, it allows following the movement of single molecules of two different protein species in live cells with a localization precision beyond the diffraction limit of light in real time...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28924180/%C3%AE-snap-is-expressed-in-mouse-ovarian-granulosa-cells-and-plays-a-key-role-in-folliculogenesis-and-female-fertility
#11
Alexis Arcos, Matilde de Paola, Diego Gianetti, Diego Acuña, Zahady D Velásquez, María Paz Miró, Gabriela Toro, Bryan Hinrichsen, Rosa Iris Muñoz, Yimo Lin, Gonzalo A Mardones, Pamela Ehrenfeld, Francisco J Rivera, Marcela A Michaut, Luis Federico Batiz
The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28920820/morbidity-and-mortality-of-wild-turtles-at-a-north-carolina-wildlife-clinic-a-10-year-retrospective
#12
Alexandra Sack, Eric Butler, Peter Cowen, Gregory A Lewbart
The medical records from 1,847 wild turtle patients seen between 2005 and 2014 by the Turtle Rescue Team at the North Carolina State University College of Veterinary Medicine were analyzed. Eastern box turtles (Terrapene carolina carolina; n = 947), yellow-bellied sliders (Trachemys scripta scripta; n = 301), cooters ( Pseudemys spp.; n = 235), common snapping turtles (Chelydra serpentina; n = 165), and eastern painted turtles (Chrysemys picta; n = 93) made up 94.3% of all patients. Patient admissions peaked in May when 25...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28917325/comparison-of-five-diagnostic-tests-for-giardia-duodenalis-in-fecal-samples-from-young-dogs
#13
Fabienne D Uehlinger, S Ali Naqvi, Spencer J Greenwood, J Trenton McClure, Gary Conboy, Ryan O'Handley, Herman W Barkema
Five diagnostic tests were compared for the diagnosis of Giardia duodenalis in fecal samples of young dogs. Fecal samples were collected from 136 healthy dogs <1year old and examined using immunofluorescence antibody microscopy (IFA) after sucrose gradient centrifugation, zinc sulfate centrifugal flotation technique (ZSCT), SNAP(®)Giardia test, and ProSpecT(®)Giardia EZ Microplate assay. In addition, polymerase chain reaction (PCR) of the 16S rRNA gene was performed. Kappa (κ) statistic was calculated to assess diagnostic agreement between the IFA and each test...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28913556/high-dose-vitamin-d3-supplementation-decreases-the-number-of-colonic-cd103-dendritic-cells-in-healthy-subjects
#14
Nina Friis Bak, M Bendix, S Hald, L Reinert, M K Magnusson, J Agnholt
PURPOSE: Vitamin D may induce tolerance in the intestinal immune system and has been shown to regulate the phenotype of tolerogenic intestinal dendritic cells (DCs) in vitro. It is unknown whether vitamin D supplementation affects human intestinal DCs in vivo, and we aimed to investigate the tolerability and effect on intestinal CD103(+)DCs of high-dose vitamin D3 treatment in healthy subjects. METHODS: Ten healthy subjects received a total of 480,000 IU oral vitamin D3 over 15 days and colonic biopsies were obtained before and after intervention by endoscopy...
September 14, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28911638/assessment-of-antibacterial-drug-residues-in-milk-for-consumption-in-kosovo
#15
Adem Rama, Lorena Lucatello, Cristiana Benetti, Guglielmo Galina, Drita Bajraktari
The objective of this study was to assess the occurrence of drug residues in the raw milk collected from individual farms and milk collection points during 2009-2010 in six different major regions of Kosovo (Prishtinë, Gjilan, Mitrovicë, Pejë, Gjakovë, Prizren). In the present study, a total of 1734 raw milk samples were collected, and qualitatively screened with two different tests, the Delvotest SP assay and an enzyme-linked receptor-binding assay (SNAP). Overall, 106 (6.11%) out of 1734 samples examined with Delvotest SP contained possible drug residues (5...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911282/what-are-we-injecting-with-our-drugs
#16
A F Merry, D A Gargiulo, L E Fry
In preparation for a case, an anaesthetist opened a 20 ml glass vial of propofol and aspirated the propofol into a syringe via a blunt drawing-up needle. Increased resistance was felt with aspiration. On inspection, a shard of glass was found at the tip of the drawing-up needle. The shard was presumed to be from the propofol ampoule, and to have fallen into the solution upon snapping open its glass tip. This illustrative case raises the issue of contamination of drugs by particles introduced during the drawing-up process...
September 2017: Anaesthesia and Intensive Care
https://www.readbyqxmd.com/read/28905964/development-and-applications-of-a-near-infrared-dye-benzylguanine-conjugate-to-specifically-label-snap-tagged-proteins
#17
Xinbo Song, Hui Bian, Chao Wang, Mingyu Hu, Ning Li, Yi Xiao
Near-infrared (NIR) fluorescent probes are advantageous over visible ones, for they can avoid the interference from the short-wavelength background emission in biological systems. However, there are a very limited number of NIR probes that can specifically label target proteins in living cells. In this work, a series of long-wavelength dyes (N-NIR, S-NIR, and K-NIR) analogous to the novel Changsha NIR family are synthesized conveniently through a new approach that is different from the previously reported one...
September 14, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28894758/the-latarjet-procedure-at-the-national-football-league-scouting-combine-an-imaging-and-performance-analysis
#18
George F LeBus, Jorge Chahla, George Sanchez, Ramesses Akamefula, Gilbert Moatshe, Alexandra Phocas, Mark D Price, James M Whalen, Robert F LaPrade, Matthew T Provencher
BACKGROUND: The Latarjet procedure is commonly performed in the setting of glenoid bone loss for treatment of recurrent anterior shoulder instability; however, little is known regarding the outcomes of this procedure in elite American football players. PURPOSE: (1) Determine the prevalence, clinical features, and imaging findings of elite college football athletes who present to the National Football League (NFL) Combine with a previous Latarjet procedure and (2) describe these athletes' performance in the NFL in terms of draft status and initial playing time...
September 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28893819/agriculture-and-health-sectors-collaborate-in-addressing-population-health
#19
Arthur Kaufman, Jon Boren, Sonja Koukel, Francisco Ronquillo, Cindy Davies, Carolina Nkouaga
PURPOSE: Population health is of growing importance in the changing health care environment. The Cooperative Extension Service, housed in each state's land grant university, has a major impact on population health through its many community-based efforts, including the Supplemental Nutrition Assistance Program - Education (SNAP-Ed) nutrition programs, 4-H youth engagement, health and wellness education, and community development. Can the agricultural and health sectors, which usually operate in parallel, mostly unknown to each other, collaborate to address population health? We set out to provide an overview of the collaboration between the Cooperative Extension Service and the health sector in various states and describe a case study of 1 model as it developed in New Mexico...
September 2017: Annals of Family Medicine
https://www.readbyqxmd.com/read/28891646/synergic-click-boronate-thiosemicarbazone-system-for-fast-and-irreversible-bioorthogonal-conjugation-in-live-cells
#20
Burcin Akgun, Caishun Li, Yubin Hao, Gareth Lambkin, Ratmir Derda, Dennis G Hall
Fast, high-yielding and selective bioorthogonal 'click' reactions employing non-toxic reagents are in high demand for their great utility in the bioconjugation of biomolecules in live cells. Although a number of click reactions were developed for this purpose, many are associated with drawbacks and limitations that justify the development of alternative systems for both single- or dual-labeling applications. Recent reports highlighted the potential of boronic ester formation as a bioorthogonal click reaction between abiotic boronic acids and diols...
September 11, 2017: Journal of the American Chemical Society
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