keyword
https://read.qxmd.com/read/38168360/a-heptavalent-o-antigen-bioconjugate-vaccine-exhibits-differential-functional-antibody-responses-against-diverse-klebsiella-pneumoniae-isolates
#21
Paeton L Wantuch, Cory J Knoot, Lloyd S Robinson, Evgeny Vinogradov, Nichollas E Scott, Christian M Harding, David A Rosen
Klebsiella pneumoniae is a concerning pathogen that is now the leading cause of neonatal sepsis and is increasingly difficult to treat due to heightened antibiotic resistance. Thus, there is an urgent need for preventive and effective immunotherapies targeting K. pneumoniae . Vaccination represents a tractable approach to combat this resistant bacterium in some settings; however, there is currently not a licensed K. pneumoniae vaccine available. K. pneumoniae surface polysaccharides, including the terminal O-antigen polysaccharides of lipopolysaccharide, have long been attractive candidates for vaccine inclusion...
December 12, 2023: bioRxiv
https://read.qxmd.com/read/38139119/depolymerisation-of-the-klebsiella-pneumoniae-capsular-polysaccharide-k21-by-klebsiella-phage-k5
#22
JOURNAL ARTICLE
Anna A Lukianova, Mikhail M Shneider, Peter V Evseev, Mikhail V Egorov, Anastasiya A Kasimova, Anna M Shpirt, Alexander S Shashkov, Yuriy A Knirel, Elena S Kostryukova, Konstantin A Miroshnikov
Klebsiella pneumoniae is a pathogen associated with various infection types, which often exhibits multiple antibiotic resistance. Phages, or bacterial viruses, have an ability to specifically target and destroy K. pneumoniae , offering a potential means of combatting multidrug-resistant infections. Phage enzymes are another promising therapeutic agent that can break down bacterial capsular polysaccharide, which shields K. pneumoniae from the immune response and external factors. In this study, Klebsiella phage K5 was isolated; this phage is active against Klebsiella pneumoniae with the capsular type K21...
December 9, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38091237/multidrug-resistant-clinical-k-pneumoniae-st16-st218-and-st283-and-emergence-of-pandrug-resistant-kpc-positive-st6434-k2-lineage-in-iraq
#23
JOURNAL ARTICLE
Hasan Falah Lahij, Safaa Abed Lateef Almeani
BACKGROUND AND AIM: The increasing incidence of Klebsiella pneumoniae infections, both in the community and in hospitals, is a huge health problem. This is due to the increasing resistance of the bacteria to antibiotics and biofilm formation, as well as the presence of a capsule. This study focuses on two main objectives: to survey the most common capsular types in local isolates for the first time in Anbar, Iraq, on molecular level and to distinguish between infectious pathogen strains using multilocus sequence typing (MLST) for more efficient epidemiological and surveillance analysis, in order to determine the source of these strains (invasive or purebred)...
December 13, 2023: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/38072846/selenium-nanoparticles-coated-bacterial-polysaccharide-with-potent-antimicrobial-and-anti-lung-cancer-activities
#24
JOURNAL ARTICLE
Nourhan S Shehata, Bassma H Elwakil, Salma S Elshewemi, Doaa A Ghareeb, Zakia A Olama
Bacterial exopolysaccharides are homopolymeric or heteropolymeric polysaccharides with large molecular weights (10-1000 kDa). Exopolysaccharides' functional uses and potential have revolutionized the industrial and medicinal industries. Hence, the aim of the present study was to optimize the production of bacterial exopolysaccharide and apply it as a capping agent for selenium nanoparticles synthesis. Exopolysaccharide (EPS) producing Lactic acid bacteria (LAB) were isolated from dairy products then biochemically characterized and assessed for their potential antimicrobial effect...
December 10, 2023: Scientific Reports
https://read.qxmd.com/read/38042959/klebsiella-pneumoniae-clinical-isolates-with-features-of-both-multidrug-resistance-and-hypervirulence-have-unexpectedly-low-virulence
#25
JOURNAL ARTICLE
Travis J Kochan, Sophia H Nozick, Aliki Valdes, Sumitra D Mitra, Bettina H Cheung, Marine Lebrun-Corbin, Rachel L Medernach, Madeleine B Vessely, Jori O Mills, Christopher M R Axline, Julia A Nelson, Ethan M VanGosen, Timothy J Ward, Egon A Ozer, David van Duin, Liang Chen, Barry N Kreiswirth, S Wesley Long, James M Musser, Zackery P Bulman, Richard G Wunderink, Alan R Hauser
Klebsiella pneumoniae has been classified into two types, classical K. pneumoniae (cKP) and hypervirulent K. pneumoniae (hvKP). cKP isolates are highly diverse and important causes of nosocomial infections; they include globally disseminated antibiotic-resistant clones. hvKP isolates are sensitive to most antibiotics but are highly virulent, causing community-acquired infections in healthy individuals. The virulence phenotype of hvKP is associated with pathogenicity loci responsible for siderophore and hypermucoid capsule production...
December 2, 2023: Nature Communications
https://read.qxmd.com/read/38031597/modified-impedance-sensing-system-determination-of-virulence-characteristics-of-pathogenic-bacteria-klebsiella-species
#26
REVIEW
Duyen Thi Ngoc Huynh, Min-Cheol Lim, Rishi Kumar Jaiswal
An impedance sensing system is a family of biosensors that measure changes in electrical impedance to perform their functions. Physical and chemical changes in the impedance of the sensing element, such as changes in the concentration of a target analyte or changes in the physical properties of the sensing element, can result in changes in the impedance of the sensing element. Many impedance biosensors have been developed for the detection of pathogens in the past few decades. Several types of biosensors have been developed for the detection of infections, including transduction elements, biorecognition components, and electrochemical approaches...
December 2023: Indian Journal of Microbiology
https://read.qxmd.com/read/38010271/characterization-of-klebsiella-pneumoniae-extracellular-polysaccharides
#27
JOURNAL ARTICLE
Saroj Khadka, Brooke E Ring, Drew A Pariseau, Laura A Mike
Klebsiella pneumoniae is a clinically significant, Gram-negative pathogen in which the production of extracellular polysaccharides is a key virulence factor. Extracellular polysaccharides such as the capsule and its mucoviscosity play a significant role in K. pneumoniae infection. In this article, we explain several standard protocols used to characterize the extracellular polysaccharides of K. pneumoniae. Several of these protocols are adapted specifically for K. pneumoniae and describe methods to purify and quantify the extracellular polysaccharide of K...
November 2023: Current protocols
https://read.qxmd.com/read/37982617/-klebsiella-pneumoniae-tolc-contributes-to-antimicrobial-resistance-exopolysaccharide-production-and-virulence
#28
JOURNAL ARTICLE
X Renee Bina, Yuding Weng, James Budnick, Mia E Van Allen, James E Bina
Klebsiella pneumoniae is a Gram-negative bacterium that causes a variety of human diseases, ranging from pneumonia to urinary tract infections and invasive diseases. The emergence of K. pneumoniae strains that are resistant to multiple antibiotics has made treatment more complex and led to K. pneumoniae becoming a global health threat. Addressing this threat necessitates the development of new therapeutic strategies to combat this pathogen, including strategies to overcome antimicrobial resistance and therapeutics for novel targets such as antivirulence...
November 20, 2023: Infection and Immunity
https://read.qxmd.com/read/37966500/a-novel-antibacterial-approach-of-cecropin-b-peptide-loaded-on-chitosan-nanoparticles-against-mdr-klebsiella-pneumoniae-isolates
#29
JOURNAL ARTICLE
Hend Okasha, Heba Dahroug, Abdullah E Gouda, Mohamed Abbas Shemis
Egypt has witnessed the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae, which has posed a serious healthcare challenge. The proper treatment choice for MDR-KP infections is not well determined which renders the problem more complicated, thus making the control of such infections a serious challenge for healthcare professionals. This study aims to encapsulate the cationic antimicrobial peptide; Cecropin-B (Cec-B), to increase its lifetime, drug targeting, and efficacy and study the antimicrobial effect of free and encapsulated recombinant rCec-B peptide on multidrug-resistant K...
November 15, 2023: Amino Acids
https://read.qxmd.com/read/37964369/genome-sequence-of-a-virulent-and-hypermucoviscous-like-klebsiella-michiganensis-clinical-isolate
#30
JOURNAL ARTICLE
Alejandro Alvarado-Delgado, Nadia Rodríguez-Medina, Alejandro Sánchez-Pérez, Elsa María Tamayo-Legorreta, Jackeline Cerón-Lopez, Rayo Morfin-Otero, Ulises Garza-Ramos
OBJECTIVES: The hypermucoviscous-like phenotype has been described in Klebsiella pneumoniae species complex (KpSC) and was described as a contributor of increased virulence. This study described the characterization and whole-genome sequencing of an antibiotic susceptible and hypermucoviscous-like Klebsiella michiganensis 9273 clinical isolate. DATA DESCRIPTION: Here, we report the genome sequence of a K. michiganensis clinical isolate obtained from a urinary tract infection exhibiting the hypermucoviscous-like phenotype...
November 14, 2023: BMC Research Notes
https://read.qxmd.com/read/37953580/formation-ability-and-drug-resistance-mechanism-of-klebsiella-pneumoniae-biofilm-and-capsule-for-multidrug-resistant
#31
JOURNAL ARTICLE
Jiayu Liang, Shanshan Lin, Lanbo He
This study was to explore the formation ability of biofilm and capsule and the drug resistance mechanism for multidrug-resistant Klebsiella pneumoniae. firstly, 55 strains of K. pneumoniae were screened out from the body fluid specimens of the laboratory. The strains were drug-resistant, and the characteristics of clinical infections of these strains were analyzed. Secondly, all strains were tested for the presence of biofilms and capsules, and then the deoxyribonucleic acid (DNA) genomes of the strains extracted were detected using polymerase chain reaction (PCR) technology...
October 31, 2023: Cellular and Molecular Biology
https://read.qxmd.com/read/37944808/comparative-genomic-analyses-of-klebsiella-pneumoniae-k57-capsule-serotypes-isolated-from-bovine-mastitis-in-china
#32
JOURNAL ARTICLE
Jingyue Yang, Yindi Xiong, Herman W Barkema, Xiaofang Tong, Yushan Lin, Zhaoju Deng, John P Kastelic, Diego B Nobrega, Yue Wang, Bo Han, Jian Gao
Klebsiella pneumoniae can cause severe clinical mastitis in dairy cows, with K. pneumoniae type K57 (K57-KP) being the most common capsular serotype. To identify virulence factors and antimicrobial resistance (AMR) genes of K57-KP with varying virulence, Galleria mellonella (greater wax moth) larvae were infected as a screening model to characterize virulence of 90 K57-KP strains, with 10 and 11 strains defined as virulent or attenuated, respectively, based on larval survival rates. Next, virulence of these 21 isolates was subsequently confirmed in adhesion and lactate dehydrogenase release assays, using bovine mammary epithelial cells cultured in vitro...
November 7, 2023: Journal of Dairy Science
https://read.qxmd.com/read/37889096/genetic-manipulation-of-klebsiella-pneumoniae
#33
JOURNAL ARTICLE
Brooke E Ring, Saroj Khadka, Drew A Pariseau, Laura A Mike
Klebsiella pneumoniae is a Gram-negative, rod-shaped bacterium commonly found in the human intestine. Although it typically exists as part of the normal flora, it can also cause healthcare-associated infections with severe consequences. Understanding the specific genes responsible for its virulence through genetic manipulation is crucial for potential therapeutic interventions. However, manipulating K. pneumoniae presents challenges due to its exopolysaccharide capsule. This article presents a comprehensive collection of protocols designed to facilitate the genetic manipulation of K...
October 2023: Current protocols
https://read.qxmd.com/read/37882584/broad-range-capsule-dependent-lytic-sugarlandvirus-against-klebsiella-sp
#34
JOURNAL ARTICLE
Robby Concha-Eloko, Pilar Barberán-Martínez, Rafael Sanjuán, Pilar Domingo-Calap
The emergence of antibiotic-resistant bacteria has become a serious global health threat requiring the development of novel treatments. Klebsiella pneumoniae is an encapsulated bacterium considered a major concern due to its high resistance, prevalence, and mortality rates. Phage therapy has been proposed as a very promising alternative to combat infections by Klebsiella sp. infections. However, most of the Klebsiella phages described so far present a high specificity, infecting one or a few capsular types due to the presence of depolymerases in their genomes, which limits their therapeutic potential...
October 26, 2023: Microbiology Spectrum
https://read.qxmd.com/read/37869673/prevalence-of-multidrug-resistant-hypervirulent-klebsiella-pneumoniae-without-defined-hypervirulent-biomarkers-in-anhui-china-a-new-dimension-of-hypervirulence
#35
JOURNAL ARTICLE
Md Roushan Ali, Yu Yang, Yuanyuan Dai, Huaiwei Lu, Zhien He, Yujie Li, Baolin Sun
Klebsiella pneumoniae is an opportunistic pathogen that mainly causes nosocomial infections and hospital-associated pneumonia in elderly and immunocompromised people. However, multidrug-resistant hypervirulent K. pneumoniae (MDR-hvKp) has emerged recently as a serious threat to global health that can infect both immunocompromised and healthy individuals. It is scientifically established that plasmid-mediated regulator of mucoid phenotype genes ( rmpA and rmpA2 ) and other virulence factors (aerobactin and salmochelin) are mainly responsible for this phenotype...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/37789842/genomic-analysis-of-carbapenem-resistant-hypervirulent-klebsiella-pneumoniae-in-a-chinese-tertiary-hospital
#36
JOURNAL ARTICLE
Lan Chen, Ying Zhou, Shanshan Wang, Chunyang Wu, Peiyao Zhou, Bingjie Wang, Zhu Chen, Fangyou Yu
BACKGROUND: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) has become a clinical crisis and is associated with significant morbidity and mortality. The prevalence of CR-hvKP has trended upward since 2010. This study aims to describe the clinical and genomic characteristics of CR-hvKP collected from a tertiary hospital in eastern China, from August 2020 to October 2021. METHODS: We tested the susceptibility to common antibiotics in these isolates to feature the antibiotic-resistant phenotypes...
2023: Infection and Drug Resistance
https://read.qxmd.com/read/37751847/whole-genome-sequence-of-carbapenem-resistant-hypermucoviscous-klebsiella-pneumoniae-k2-st375-with-bla-ndm-harboring-conjugative-incx3-and-plvpk-like-virulence-plasmids-from-a-patient-in-china
#37
JOURNAL ARTICLE
Yongxiang Wang, Helan Liu, Anlin Chen, Song Gao, Jian Huang, Zeling Dong, Changwei Lei, Zuyi Chen, Xun Min, Zehui Chen
OBJECTIVES: Carbapenem-resistant hypermucoviscous Klebsiella pneumoniae (CR-HMKP) poses unprecedented public health challenges. However, genomic information regarding the CR-HMKP K2-ST375 strain is scarce. The aim of this study was to characterize the whole genome sequence of the CR-HMKP K2-ST375 strain Kp0179 isolated from a male patient in China. METHODS: The whole genome of Kp0179 was sequenced using the DNBSEQ and Pacific Biosciences RSII platforms. The capsular serotype, multilocus sequence typing (MLST), antimicrobial resistance genes, and virulence factors were determined using available databases and bioinformatics tools...
September 24, 2023: Journal of Global Antimicrobial Resistance
https://read.qxmd.com/read/37744914/regulation-of-biofilm-formation-in-klebsiella-pneumoniae
#38
REVIEW
Yin Li, Ming Ni
Klebsiella pneumoniae is an important Gram-negative opportunistic pathogen that is responsible for a variety of nosocomial and community-acquired infections. Klebsiella pneumoniae has become a major public health issue owing to the rapid global spread of extensively-drug resistant (XDR) and hypervirulent strains. Biofilm formation is an important virulence trait of K. pneumoniae . A biofilm is an aggregate of microorganisms attached to an inert or living surface by a self-produced exo-polymeric matrix that includes proteins, polysaccharides and extracellular DNA...
2023: Frontiers in Microbiology
https://read.qxmd.com/read/37741345/exploring-the-enzymatic-activity-of-depolymerase-gp531-from-klebsiella-pneumoniae-jumbo-phage-rak2
#39
JOURNAL ARTICLE
Algirdas Noreika, Jonita Stankevičiūtė, Rasa Rutkienė, Rolandas Meškys, Laura Kaliniene
Klebsiella pneumoniae poses a major global challenge due to its virulence, multidrug resistance, and nosocomial nature. Thus, bacteriophage-derived proteins are extensively being investigated as a means to combat this bacterium. In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 (RaK2). We used two different methods to modify the reducing end of the oligosaccharides released during capsule hydrolysis with gp531. Subsequent acidic cleavage with TFA, followed by TLC and HPLC-MS analyses, revealed that RaK2 gp531 is a β-(1→4)-endoglucosidase...
September 21, 2023: Virus Research
https://read.qxmd.com/read/37727625/sputum-gram-stain-of-hypervirulent-klebsiella-pneumoniae
#40
Yuji Nishihara, Takahito Nakamura, Yuki Suzuki, Kei Kasahara
Hypervirulent Klebsiella pneumoniae has virulence genes relevant to capsule overproduction, which could lead to the finding of thick transparent area ("halo") in Gram stain. In sputum Gram stain, observation of a large Gram-negative rod with a thick capsule may not only indicate that it is K. pneumoniae but also that it is a highly virulent strain.
September 2023: Journal of General and Family Medicine
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