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klebsiella pneumoniae capsule

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https://www.readbyqxmd.com/read/29753130/clonal-dissemination-of-carbapenemase-producing-klebsiella-pneumoniae-two-distinct-sub-lineages-of-sequence-type-11-carrying-bla-kpc-2-and-bla-oxa-48
#1
Min-Chi Lu, Hui-Ling Tang, Chien-Shun Chiou, Yao-Chen Wang, Ming-Ko Chiang, Yi-Chyi Lai
OBJECTIVES: The global spread of carbapenem-resistant Klebsiella pneumoniae (CR-Kp) has become a massive threat to human health. We here investigated the clonal relatedness of CR-Kp strains in central Taiwan. METHODS: CR-Kp were prospectively collected from inpatients referred to Chung Shan Medical University Hospital (CSMUH) during Sep. 2011 to Dec. 2015. The presence of carbapenemase genes, including blaKPC-2 , blaVIM-1 , blaNDM-1 , and blaOXA-48 was analyzed with PCR and sequence determination...
May 9, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29744043/genome-based-analysis-of-carbapenemase-producing-klebsiella-pneumoniae-isolates-from-german-hospital-patients-2008-2014
#2
Laura Becker, Martin Kaase, Yvonne Pfeifer, Stephan Fuchs, Annicka Reuss, Anja von Laer, Muna Abu Sin, Miriam Korte-Berwanger, Sören Gatermann, Guido Werner
Background: By using whole genome sequence data we aimed at describing a population snapshot of carbapenemase-producing K. pneumoniae isolated from hospitalized patients in Germany between 2008 and 2014. Methods: We selected a representative subset of 107 carbapenemase-producing K. pneumoniae clinical isolates possessing the four most prevalent carbapenemase types in Germany (KPC-2, KPC-3, OXA-48, NDM-1). Isolates were processed via illumina NGS. Data were analysed using different SNP-based mapping and de-novo assembly approaches...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29712730/pulmonary-surfactant-promotes-virulence-gene-expression-and-biofilm-formation-in-klebsiella-pneumoniae
#3
Graham G Willsey, Sebastian Ventrone, Kristin C Schutz, Aaron M Wallace, John W Ribis, Benjamin T Suratt, Matthew J Wargo
The interactions between K lebsiella pneumoniae and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional response of K. pneumoniae MGH 78578 to purified pulmonary surfactant. This work revealed changes within the K. pneumoniae transcriptome that likely contribute to host colonization, adaptation, and virulence in vivo Notable transcripts expressed under these conditions include genes involved in capsule synthesis, LPS modification, antibiotic resistance, biofilm formation, and metabolism...
April 30, 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29618504/kaptive-web-user-friendly-capsule-and-lipopolysaccharide-serotype-prediction-for-klebsiella-genomes
#4
Ryan R Wick, Eva Heinz, Kathryn E Holt, Kelly L Wyres
As whole genome sequencing becomes an established component of the microbiologist's toolbox, it is imperative that researchers, clinical microbiologists and public health professionals have access to genomic analysis tools for rapid extraction of epidemiologically and clinically relevant information. For the gram-negative hospital pathogens such as Klebsiella pneumoniae , initial efforts have focused on detection and surveillance of antimicrobial resistance genes and clones. However, with the resurgence of interest in alternative infection control strategies targeting Klebsiella surface polysaccharides, the ability to extract information about these antigens is increasingly important...
April 4, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29615497/novel-broadly-reactive-anticapsular-antibodies-against-carbapenem-resistant-klebsiella-pneumoniae-protect-from-infection
#5
Elizabeth Diago-Navarro, Michael P Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M S Seco, Guozhi Xiao, Aniska Chikhalya, Peter H Seeberger, Bettina C Fries
Carbapenem-resistant (CR) sequence type 258 (ST258) Klebsiella pneumoniae has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of K...
April 3, 2018: MBio
https://www.readbyqxmd.com/read/29535199/antibody-mediated-killing-of-carbapenem-resistant-st258-klebsiella-pneumoniae-by-human-neutrophils
#6
Scott D Kobayashi, Adeline R Porter, Brett Freedman, Ruchi Pandey, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29479519/evaluating-different-virulence-traits-of-klebsiella-pneumoniae-using-dictyostelium-discoideum-and-zebrafish-larvae-as-host-models
#7
Andrés E Marcoleta, Macarena A Varas, Javiera Ortiz-Severín, Leonardo Vásquez, Camilo Berríos-Pastén, Andrea V Sabag, Francisco P Chávez, Miguel L Allende, Carlos A Santiviago, Octavio Monasterio, Rosalba Lagos
Multiresistant and invasive hypervirulent Klebsiella pneumoniae strains have become one of the most urgent bacterial pathogen threats. Recent analyses revealed a high genomic plasticity of this species, harboring a variety of mobile genetic elements associated with virulent strains, encoding proteins of unknown function whose possible role in pathogenesis have not been addressed. K. pneumoniae virulence has been studied mainly in animal models such as mice and pigs, however, practical, financial, ethical and methodological issues limit the use of mammal hosts...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29429073/non-capsulated-mutants-of-a-chemical-producing-klebsiella-pneumoniae-strain
#8
Dong Wei, Yuki Yuminaga, Jiping Shi, Jian Hao
OBJECTIVES: To investigate the outcomes of capsule lost on cell transformation efficiency and chemicals (1,3-propanediol, 2,3-butanediol, and 2-ketogluconic acid) production by Klebsiella pneumoniae. RESULTS: The cps gene cluster showed low sequence homology with pathogenic strains. The wza is a highly conserved gene in the cps cluster that encodes an outer membrane protein. A non-capsulated mutant was constructed by deletion of wza. Phenotype studies demonstrated that non-capsulated cells were less buoyant and easy to sediment...
April 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29381692/rhinoscleroma-pathogenesis-the-type-k3-capsule-of-klebsiella-rhinoscleromatis-is-a-virulence-factor-not-involved-in-mikulicz-cells-formation
#9
Barbara Corelli, Ana S Almeida, Fabiane Sonego, Virginia Castiglia, Cindy Fevre, Sylvain Brisse, Philippe J Sansonetti, Régis Tournebize
Rhinoscleroma is a human specific chronic granulomatous infection of the nose and upper airways caused by the Gram-negative bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. Although considered a rare disease, it is endemic in low-income countries where hygienic conditions are poor. A hallmark of this pathology is the appearance of atypical foamy monocytes called Mikulicz cells. However, the pathogenesis of rhinoscleroma remains poorly investigated. Capsule polysaccharide (CPS) is a prominent virulence factor in bacteria...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29326011/neonatal-intestinal-colonization-with-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-a-5-year-follow-up-study
#10
V Nordberg, K Jonsson, C G Giske, A Iversen, O Aspevall, B Jonsson, A Camporeale, M Norman, L Navér
OBJECTIVES: To analyse Klebsiella pneumoniae (KP) isolates from an outbreak of extended-spectrum β-lactamase (ESBL)-producing KP and Escherichia coli (EC) among infants admitted to neonatal intensive care units and to determine the duration of the intestinal colonization. METHODS: We performed a prospective cohort study of intestinal ESBL-KP/ESBL-EC colonized neonates after a 5-month outbreak in two neonatal intensive care units. Whole genome sequencing, multilocus sequence typing, core genome multilocus sequence typing, pulsed-field electrophoresis and PCR for blaCTX-M were performed on the first isolates...
January 8, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29319515/the-determination-and-correlation-of-various-virulence-genes-esbl-serum-bactericidal-effect-and-biofilm-formation-of-clinical-isolated-classical-klebsiella-pneumoniae-and-hypervirulent-klebsiella-pneumoniae-from-respiratory-tract-infected-patients
#11
Rambha K Shah, Zhao H Ni, Xiao Y Sun, Guo Q Wang, Fan Li
Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients...
December 4, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29297821/isolation-identification-and-genome-analysis-of-lung-pathogenic-klebsiella-pneumoniae-lpkp-in-forest-musk-deer
#12
Wei Zhao, Qing Tian, Yan Luo, Yin Wang, Ze-Xiao Yang, Xue-Ping Yao, Jian-Guo Cheng, Xin Zhou, Wu-You Wang
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis. Median lethal dose and histopathologic analysis were used to demonstrate pathogenicity of the organism in mice. Furthermore, a draft genome of LPKP was sequenced, and its virulence genes were detected. One hundred and twenty-two virulence genes encoded determinant of capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, and urease...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29205138/virulence-genes-in-isolates-of-klebsiella-pneumoniae-from-the-uk-during-2016-including-among-carbapenemase-gene-positive-hypervirulent-k1-st23-and-non-hypervirulent-types-st147-st15-and-st383
#13
Jane F Turton, Zoë Payne, Amy Coward, Katie L Hopkins, Jack A Turton, Michel Doumith, Neil Woodford
PURPOSE: Klebsiella pneumoniae is a concern because of its multidrug resistance and the ability of hypervirulent types, especially capsular type K1-clonal complex 23 (K1-CC23), to cause community-acquired, life-threatening infections. Hypervirulent types carry an array of virulence genes including rmpA/rmpA2, coding for capsule up-regulation. We sought to identify isolates carrying these elements among submissions to the UK national reference laboratory during 2016. METHODOLOGY: Virulence elements and carbapenemase genes were sought by PCR or from whole genome sequences...
January 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29158434/high-levels-of-cyclic-di-gmp-in-klebsiella-pneumoniae-attenuate-virulence-in-the-lung
#14
David A Rosen, Joy Twentyman, David A Hunstad
The bacterial second messenger bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) has been shown to influence the expression of virulence factors in certain pathogenic bacteria, but little is known about its activity in the increasingly antibiotic-resistant pathogen Klebsiella pneumoniae Here, the expression in K. pneumoniae of a heterologous diguanylate cyclase increased the bacterial c-di-GMP concentration and attenuated pathogenesis in murine pneumonia. This attenuation remained evident in mice lacking the c-di-GMP sensor STING, indicating that the high c-di-GMP concentration exerted its influence not on host responses but on bacterial physiology...
February 2018: Infection and Immunity
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#15
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29051525/virulence-characteristics-of-carbapenem-resistant-klebsiella-pneumoniae-strains-from-patients-with-necrotizing-skin-and-soft-tissue-infections
#16
Fiorella Krapp, Andrew R Morris, Egon A Ozer, Alan R Hauser
Two types of Klebsiella pneumoniae (KP) strains are currently emerging: hypervirulent (hvKP) strains and carbapenem-resistant (CR-KP) strains. To date, these two strain types rarely overlap. Recent reports, however, suggest that CR-KP strains are increasing in virulence. hvKP strains frequently present as highly invasive infections, such as necrotizing skin and soft tissue infections (NSSTI). To examine whether CR-KP strains with features of hvKP were present in our U.S. hospital, we retrospectively identified four cases of CR-KP NSSTI diagnosed between January 2012 and January 2016...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28936426/comparison-of-diabetic-and-non-diabetic-human-leukocytic-responses-to-different-capsule-types-of-klebsiella-pneumoniae-responsible-for-causing-pyogenic-liver-abscess
#17
I Russel Lee, Ethel Sng, Kok-Onn Lee, James S Molton, Monica Chan, Shirin Kalimuddin, Ezlyn Izharuddin, David C Lye, Sophia Archuleta, Yunn-Hwen Gan
The major risk factor for Klebsiella liver abscess (KLA) is type 2 diabetes mellitus (DM), but the immunological mechanisms involved in the increased susceptibility are poorly defined. We investigated the responses of neutrophils and peripheral blood mononuclear cells (PBMCs) to hypervirulent Klebsiella pneumoniae (hvKP), the causative agent of KLA. DNA and myeloperoxidase levels were elevated in the plasma of KLA patients compared to uninfected individuals indicating neutrophil activation, but diabetic status had no effect on these neutrophil extracellular trap (NET) biomarkers in both subject groups...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28915364/nosocomial-multidrug-resistant-klebsiella-pneumoniae-strains-isolated-from-mexico-city-produce-robust-biofilms-on-abiotic-surfaces-but-not-on-human-lung-cells
#18
Martha Lorena Ostria-Hernandez, Karla Cecilia Juárez-de la Rosa, Patricia Arzate-Barbosa, Antonino Lara-Hernández, Fuminori Sakai, J Antonio Ibarra, Graciela Castro-Escarpulli, Jorge E Vidal
BACKGROUND: Klebsiella pneumoniae (Kpn) strains are a leading cause of hospital-acquired infections, including ventilator-associated pneumonia. Resistance to antibiotics, biofilm formation, and the production of certain fimbriae play an important role in the pathogenesis. AIM: We investigated the genetic relatedness, antibiotic resistance, virulence potential, and ability to form biofilms of Kpn strains isolated from hospital-acquired infections (n = 76). Strains were isolated at three major hospitals serving the largest metropolitan urban area in Mexico City, Mexico...
September 15, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28861522/a-serendipitous-mutation-reveals-the-severe-virulence-defect-of-a-klebsiella-pneumoniae-fepb-mutant
#19
Michelle Palacios, Christopher A Broberg, Kimberly A Walker, Virginia L Miller
Klebsiella pneumoniae is considered a significant public health threat because of the emergence of multidrug-resistant strains and the challenge associated with treating life-threatening infections. Capsule, siderophores, and adhesins have been implicated as virulence determinants of K. pneumoniae, yet we lack a clear understanding of how this pathogen causes disease. In a previous screen for virulence genes, we identified a potential new virulence locus and constructed a mutant (smr) with this locus deleted...
July 2017: MSphere
https://www.readbyqxmd.com/read/28747788/klebsiella-pneumoniae-isolates-from-meningitis-epidemiology-virulence-and-antibiotic-resistance
#20
Yee-Huang Ku, Yin-Ching Chuang, Chi-Chung Chen, Mei-Feng Lee, Yan-Chang Yang, Hung-Jen Tang, Wen-Liang Yu
Klebsiella pneumoniae (KP) resistance to broad-spectrum cephalosporin (BSC) in meningitis is important because of limited therapeutic options. To investigate the antibiotic resistance, virulence and epidemiology of KP in meningitis, we conducted a retrospective study for 33 non-metastatic isolates, including primary meningitis (n = 20) and post-craniotomy meningitis (n = 13) collected from 1999 to 2013. BSC resistance was found in 9 (27.3%) isolates, all from post-craniotomy meningitis, harboring bla SHV-5 (n = 6), bla CMY-2 (n = 2), bla DHA-1 (n = 2), and bla TEM-1B (n = 1)...
July 26, 2017: Scientific Reports
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