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klebsiella pneumoniae capsular polysaccharide

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https://www.readbyqxmd.com/read/27902401/involvement-of-camp-receptor-protein-in-biofilm-formation-fimbria-production-capsular-polysaccharide-biosynthesis-and-lethality-in-mouse-of-klebsiella-pneumoniae-serotype-k1-causing-pyogenic-liver-abscess
#1
Qin Ou, Jinming Fan, Dejian Duan, Xu Li, Jie Wang, Dongsheng Zhou, Huiying Yang, Bei Li
The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/ or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella pneumoniae capsular serotype K1 strain causing the primary pyogenic liver abscess. The phenotypes of wild-type strain, crp mutant, and complemented mutant were characterized systematically. It was disclosed that K...
November 16, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27752727/a-nanomechanical-study-of-the-effects-of-colistin-on-the-klebsiella-pneumoniae-aj218-capsule
#2
Anna Mularski, Jonathan Wilksch, Eric Hanssen, Jian Li, Takehiro Tomita, Sacha James Pidot, Tim Stinear, Frances Separovic, Dick Strugnell
Atomic force microscopy measurements of capsule thickness revealed that that the wild-type Klebsiella pneumoniae AJ218 capsular polysaccharides were rearranged by exposure to colistin. The increase in capsule thickness measured near minimum inhibitory/bactericidal concentration (MIC/MBC) is consistent with the idea that colistin displaces the divalent cations that cross-bridge adjacent lipopolysaccharide (LPS) molecules through the capsule network. Cryo-electron microscopy demonstrated that the measured capsule thickness at near MIC/MBC of 1...
October 17, 2016: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/27707941/development-of-a-colloidal-gold-based-immunochromatographic-strip-for-rapid-detection-of-klebsiella-pneumoniae-serotypes-k1-and-k2
#3
L Kristopher Siu, Yu-Kuo Tsai, Jung-Chung Lin, Te-Li Chen, Chang-Phone Fung, Feng-Yee Chang
In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect K. pneumoniae serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable K. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips...
December 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27683817/cd36-provides-host-protection-against-klebsiella-pneumoniae-intrapulmonary-infection-by-enhancing-lps-responsiveness-and-macrophage-phagocytosis
#4
Tolani F Olonisakin, Huihua Li, Zeyu Xiong, Elizabeth J K Kochman, Minting Yu, Yanyan Qu, Mei Hulver, Jay K Kolls, Claudette St Croix, Yohei Doi, Minh-Hong Nguyen, Robert M Q Shanks, Rama K Mallampalli, Valerian E Kagan, Anuradha Ray, Roy L Silverstein, Prabir Ray, Janet S Lee
Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. Klebsiella pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified-self ligands, is a host determinant of Klebsiella pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of three distinct Klebsiella pneumoniae (hypermucoviscous K1, research K2, carbapenemase-producing ST258) strains utilized, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K...
September 28, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27565716/effect-in-virulence-of-switching-conserved-homologous-capsular-polysaccharide-genes-from-klebsiella-pneumoniae-serotype-k1-into-k20
#5
Chii-Lan Lin, Fei-Hsu Chen, Li-Yueh Huang, Jen-Chang Chang, Jiun-Han Chen, Yu-Kuo Tsai, Feng-Yee Chang, Jung-Chung Lin, L Kristopher Siu
The capsular polysaccharides in different serotypes of Klebsiella pneumoniae (KP) coded by the (CPS) gene cluster are characterized by a conserved and a hyper-variable region. We performed a virulence study by switching genes in the highly conserved region of the CPS cluster between strains. Six genes in the CPS conserved region in serotype K20, including galF, acidPPc, wzi, wza, wzb and wzc, were knocked out and replaced by the homologous genes from serotype K1. Compared to the parental K20 strain, the mutants showed a decline in lethality (LD50) in mice from 10-fold to > 10(5)-fold and were categorized in terms of the effect on virulence as low (L) for galF and acidPPC, moderate (M) for wzi, and high (H) for wza, wzb and wzc...
August 26, 2016: Virulence
https://www.readbyqxmd.com/read/27550826/identification-of-a-capsular-variant-and-characterization-of-capsular-acetylation-in-klebsiella-pneumoniae-pla-associated-type-k57
#6
Chun-Ru Hsu, Chun-Hsing Liao, Tzu-Lung Lin, Han-Ru Yang, Feng-Ling Yang, Pei-Fang Hsieh, Shih-Hsiung Wu, Jin-Town Wang
Klebsiella pneumoniae can cause community-acquired pyogenic liver abscess (PLA). Capsular polysaccharide (CPS) is important for its virulence. Among 79 capsular (K) types discovered thus far, K57 is often associated with PLA. Here, we report the identification of a K57 variant. Cps gene locus sequencing revealed differences between the K57 reference strain 4425/51 (Ref-K57) and a variant, the PLA isolate A1142. While Ref-K57 cps contained orf13 encoding a putative acetyltransferase, the insertion of a putative transposase-encoding gene at this position was detected in A1142...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27408624/comparative-genomic-analysis-of-klebsiella-pneumoniae-subsp-pneumoniae-kp617-and-pittndm01-nuhl24835-and-atcc-baa-2146-reveals-unique-evolutionary-history-of-this-strain
#7
Taesoo Kwon, Young-Hee Jung, Sanghyun Lee, Mi-Ran Yun, Won Kim, Dae-Won Kim
BACKGROUND: Klebsiella pneumoniae subsp. pneumoniae KP617 is a pathogenic strain that coproduces OXA-232 and NDM-1 carbapenemases. We sequenced the genome of KP617, which was isolated from the wound of a Korean burn patient, and performed a comparative genomic analysis with three additional strains: PittNDM01, NUHL24835 and ATCC BAA-2146. RESULTS: The complete genome of KP617 was obtained via multi-platform whole-genome sequencing. Phylogenetic analysis along with whole genome and multi-locus sequence typing of genes of the Klebsiella pneumoniae species showed that KP617 belongs to the WGLW2 group, which includes PittNDM01 and NUHL24835...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27182661/structural-determination-of-the-polysaccharide-isolated-from-biofilms-produced-by-a-clinical-strain-of-klebsiella-pneumoniae
#8
Paola Cescutti, Gianluigi De Benedetto, Roberto Rizzo
Klebsiella pneumoniae are Gram negative opportunistic pathogens producing capsular (K) polysaccharides. Seventy-seven different K antigens have been described and they are the basis for K serotyping. Capsular polysaccharides are important virulence factors and have a relevant role for the structure of biofilm communities. Nevertheless, little information is available on the polysaccharides produced in biofilm matrices by Klebsiella spp. In the present study, a clinical isolate of Klebsiella pneumoniae was grown both on cellulose membranes deposited on agar plates, where it formed an adherent biofilm, and in liquid medium, where it formed floating biofilms (flocs)...
July 22, 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/27159655/impact-of-glycemic-control-on-capsular-polysaccharide-biosynthesis-and-opsonophagocytosis-of-klebsiella-pneumoniae-implications-for-invasive-syndrome-in-patients-with-diabetes-mellitus
#9
Chen-Hsiang Lee, I-Ling Chen, Seng-Kee Chuah, Wei-Chen Tai, Chia-Chi Chang, Fang-Ju Chen, Jung-Fu Chen
Klebsiella pneumoniae (KP), with production of abundant capsular polysaccharide (CPS), is capable of causing invasive syndrome. Environmental glucose stimuli may increase CPS biosynthesis. We aimed to investigate the relationship between glycemic control and KP-mediated invasive syndrome in diabetic patients and the effect of glucose on CPS biosynthesis. Diabetic patients with community-acquired KP bacteremia were included to study the risk factors of invasive syndrome. KP-M1, a serotype-K1 KP clinical isolate, was used to examine the CPS biosynthesis and cps gene expression, and the effect of exogenous glucose on bacterial phagocytosis and killing...
October 2, 2016: Virulence
https://www.readbyqxmd.com/read/26946245/atomic-force-microscopy-of-bacteria-reveals-the-mechanobiology-of-pore-forming-peptide-action
#10
Anna Mularski, Jonathan J Wilksch, Eric Hanssen, Richard A Strugnell, Frances Separovic
Time-resolved AFM images revealed that the antimicrobial peptide (AMP) caerin 1.1 caused localised defects in the cell walls of lysed Klebsiella pneumoniae cells, corroborating a pore-forming mechanism of action. The defects continued to grow during the AFM experiment, in corroboration with large holes that were visualised by scanning electron microscopy. Defects in cytoplasmic membranes were visualised by cryo-EM using the same peptide concentration as in the AFM experiments. At three times the minimum inhibitory concentration of caerin, 'pores' were apparent in the outer membrane...
June 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/26939452/-research-advances-in-the-virulence-factors-of-klebsiella-pneumoniae-a-review
#11
REVIEW
Yanfei Kang, Pingfang Tian, Tianwei Tan
Klebsiella pneumoniae is of great attractiveness because it naturally produces a series of bulk chemicals such as 1,3-propanediol, 2,3-butanediol and 3-hydroxypropionic acid. Although this species has been fueled in recent years, its pathogenicity is considered an obstacle hindering industrial applications. Here we portray a picture of the virulence factors, including pili, receptors, capsular polysaccharides, lipopolysaccharides, and newly identified virulence factors. This review covers aspects of virulence genes, proteins, metabolic activities, as well as the mechanisms underlying infection and immune responses...
October 4, 2015: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
https://www.readbyqxmd.com/read/26904512/h-ns-nucleoid-protein-controls-virulence-features-of-klebsiella-pneumoniae-by-regulating-the-expression-of-type-3-pili-and-the-capsule-polysaccharide
#12
Miguel A Ares, José L Fernández-Vázquez, Roberto Rosales-Reyes, Ma Dolores Jarillo-Quijada, Kristine von Bargen, Javier Torres, Jorge A González-Y-Merchand, María D Alcántar-Curiel, Miguel A De la Cruz
Klebsiella pneumoniae is an opportunistic pathogen causing nosocomial infections. Main virulence determinants of K. pneumoniae are pili, capsular polysaccharide, lipopolysaccharide, and siderophores. The histone-like nucleoid-structuring protein (H-NS) is a pleiotropic regulator found in several gram-negative pathogens. It has functions both as an architectural component of the nucleoid and as a global regulator of gene expression. We generated a Δhns mutant and evaluated the role of the H-NS nucleoid protein on the virulence features of K...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/26893615/surface-antigens-contribute-differently-to-the-pathophysiological-features-in-serotype-k1-and-k2-klebsiella-pneumoniae-strains-isolated-from-liver-abscesses
#13
Kuo-Ming Yeh, Sheng-Kung Chiu, Chii-Lan Lin, Li-Yueh Huang, Yu-Kuo Tsai, Jen-Chang Chang, Jung-Chung Lin, Feng-Yee Chang, Leung-Kei Siu
BACKGROUND: The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. METHOD: To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/26890371/liver-abscess-caused-by-infection-with-community-acquired-klebsiella-quasipneumoniae-subsp-quasipneumoniae
#14
Sebastien Breurec, Benedicte Melot, Bruno Hoen, Virginie Passet, Kinda Schepers, Sylvaine Bastian, Sylvain Brisse
We report a case of pyogenic liver abscess caused by community-acquired Klebsiella quasipneumoniae subsp. quasipneumoniae. The infecting isolate had 2 prominent features of hypervirulent K. pneumoniae strains: the capsular polysaccharide synthesis region for K1 serotype and the integrative and conjugative element ICEKp1, which encodes the virulence factors yersiniabactin, salmochelin, and RmpA.
March 2016: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/26304687/emergence-of-a-hypervirulent-carbapenem-resistant-klebsiella-pneumoniae-isolate-from-clinical-infections-in-china
#15
Yawei Zhang, Ji Zeng, Wenen Liu, Feng Zhao, Zhidong Hu, Chunjiang Zhao, Qi Wang, Xiaojuan Wang, Hongbin Chen, Henan Li, Feifei Zhang, Shuguang Li, Bin Cao, Hui Wang
OBJECTIVES: Hypervirulent Klebsiella pneumoniae (hvKP) infections occur worldwide, but carbapenem-resistant hvKP strain has rarely been observed. METHODS: A retrospective study was conducted in 28 cases of carbapenem-resistant K. pneumoniae (CRKP) infections from 9 cities in China. Clinical data were collected from medical records. All the isolates were characterized by antimicrobial susceptibility testing, string test, multilocus sequence typing, and capsular genotyping...
November 2015: Journal of Infection
https://www.readbyqxmd.com/read/26282408/loss-of-hypermucoviscosity-and-increased-fitness-cost-in-colistin-resistant-klebsiella-pneumoniae-sequence-type-23-strains
#16
Myung-Jin Choi, Kwan Soo Ko
In this study, we investigated the effects of colistin resistance on virulence and fitness in hypermucoviscous (HV) Klebsiella pneumoniae sequence type 23 (ST23) strains. Colistin-resistant mutants were developed from three colistin-susceptible HV K. pneumoniae ST23 strains. The lipid A structures of strains were analyzed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Changes in HV were investigated using the string test, and extracellular polysaccharide production was quantified...
November 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26176307/potential-virulence-of-klebsiella-sp-isolates-from-enteral-diets
#17
S C L Pereira, M C D Vanetti
We aimed to evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, to support nosocomial infection control measures, especially among critical-care patients. Phenotypic determination of virulence factors, such as capsular expression on the external membrane, production of aerobactin siderophore, synthesis of capsular polysaccharide, hemolytic and phospholipase activity, and resistance to antibiotics, which are used therapeutically, were investigated in strains of Klebsiella pneumoniae and K...
September 2015: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/26077758/capsular-polysaccharide-is-involved-in-nlrp3-inflammasome-activation-by-klebsiella-pneumoniae-serotype-k1
#18
Kuo-Feng Hua, Feng-Ling Yang, Hsiao-Wen Chiu, Ju-Ching Chou, Wei-Chih Dong, Chien-Nan Lin, Chai-Yi Lin, Jin-Town Wang, Lan-Hui Li, Huan-Wen Chiu, Yi-Chich Chiu, Shih-Hsiung Wu
Klebsiella pneumoniae (strain 43816, K2 serotype) induces interleukin-1β (IL-1β) secretion, but neither the bacterial factor triggering the activation of these inflammasome-dependent responses nor whether they are mediated by NLRP3 or NLRC4 is known. In this study, we identified a capsular polysaccharide (K1-CPS) in K. pneumoniae (NTUH-K2044, K1 serotype), isolated from a primary pyogenic liver abscess (PLA K. pneumoniae), as the Klebsiella factor that induces IL-1β secretion in an NLRP3-, ASC-, and caspase-1-dependent manner in macrophages...
September 2015: Infection and Immunity
https://www.readbyqxmd.com/read/26063052/aeromonas-punctata-derived-depolymerase-improves-susceptibility-of-klebsiella-pneumoniae-biofilm-to-gentamicin
#19
Shruti Bansal, Kusum Harjai, Sanjay Chhibber
BACKGROUND: To overcome antibiotic resistance in biofilms, enzymes aimed at biofilm dispersal are under investigation. In the present study, applicability of an Aeromonas punctata derived depolymerase capable of degrading the capsular polysaccharide (CPS) of Klebsiella pneumoniae, in disrupting its biofilm and increasing gentamicin efficacy against biofilm was investigated. RESULTS: Intact biofilm of K. pneumoniae was recalcitrant to gentamicin due to lack of antibiotic penetration...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/26052327/survey-and-rapid-detection-of-klebsiella-pneumoniae-in-clinical-samples-targeting-the-rcsa-gene-in-beijing-china
#20
Derong Dong, Wei Liu, Huan Li, Yufei Wang, Xinran Li, Dayang Zou, Zhan Yang, Simo Huang, Dongsheng Zhou, Liuyu Huang, Jing Yuan
Klebsiella pneumoniae is a wide-spread nosocomial pathogen. A rapid and sensitive molecular method for the detection of K. pneumoniae in clinical samples is needed to guide therapeutic treatment. In this study, we first described a loop-mediated isothermal amplification (LAMP) method for the rapid detection of capsular polysaccharide synthesis regulating gene rcsA from K. pneumoniaein clinical samples by using two methods including real-time turbidity monitoring and fluorescence detection to assess the reaction...
2015: Frontiers in Microbiology
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