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klebsiella pneumoniae capsular polysaccharide

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https://www.readbyqxmd.com/read/29764953/fighting-antibiotic-resistant-klebsiella-pneumoniae-with-sweet-immune-targets
#1
Roberto Adamo, Immaculada Margarit
Antibiotics and vaccines have greatly impacted human health in the last century by dramatically reducing the morbidity and mortality associated with infectious diseases. The recent challenge posed by the emergence of multidrug-resistant bacteria could possibly be addressed by novel immune prophylactic and therapeutic approaches. Among the newly threatening pathogens, Klebsiella pneumoniae is particularly worrisome in the nosocomial setting, and its surface polysaccharides are regarded as promising antigen candidates...
May 15, 2018: MBio
https://www.readbyqxmd.com/read/29724185/clonal-diversity-and-genetic-profiling-of-antibiotic-resistance-among-multidrug-carbapenem-resistant-klebsiella-pneumoniae-isolates-from-a-tertiary-care-hospital-in-saudi-arabia
#2
Taher Uz Zaman, Maha Alrodayyan, Maha Albladi, Mohammed Aldrees, Mohammed Ismail Siddique, Sameera Aljohani, Hanan H Balkhy
BACKGROUND: The nexus between resistance determinants, plasmid type, and clonality appears to play a crucial role in the dissemination and survival of carbapenem-resistant Klebsiella pneumoniae (CRKP). The incidence of infections involving CRKP in Saudi Arabia is increasing and there is a need for detailed molecular profiling of this pathogen for CRKP surveillance and control. METHODS: The resistance determinants of 71 non-redundant CRKP isolates were investigated by polymerase chain reaction (PCR) and sequencing...
May 3, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29615497/novel-broadly-reactive-anticapsular-antibodies-against-carbapenem-resistant-klebsiella-pneumoniae-protect-from-infection
#3
Elizabeth Diago-Navarro, Michael P Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M S Seco, Guozhi Xiao, Aniska Chikhalya, Peter H Seeberger, Bettina C Fries
Carbapenem-resistant (CR) sequence type 258 (ST258) Klebsiella pneumoniae has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of K...
April 3, 2018: MBio
https://www.readbyqxmd.com/read/29547971/potential-targets-for-next-generation-anti-microbial-glycoconjugate-vaccines
#4
Francesca Micoli, Paolo Costantino, Roberto Adamo
Cell surface carbohydrates have been proven optimal targets for vaccine development. Conjugation of polysaccharides to a carrier protein triggers a T-cell dependent immune response to the glycan moiety. Licensed glycoconjugate vaccines are produced by chemical conjugation of capsular polysaccharides to prevent meningitis caused by meningococcus, pneumococcus and Haemophilus influenzae type b. However, other classes of carbohydrates (O-antigens, exopolysaccharides, wall/teichoic acids) represent attractive targets for developing vaccines...
March 14, 2018: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/29305504/an-igaa-umob-family-protein-from-serratia-marcescens-regulates-motility-capsular-polysaccharide-biosynthesis-and-secondary-metabolite-production
#5
Nicholas A Stella, Kimberly M Brothers, Jake D Callaghan, Angelina M Passerini, Cihad Sigindere, Preston J Hill, Xinyu Liu, Daniel J Wozniak, Robert M Q Shanks
Secondary metabolites are an important source of pharmaceuticals and key modulators of microbe-microbe interactions. The bacterium Serratia marcescens is part of the Enterobacteriaceae family of eubacteria and produces a number of biologically active secondary metabolites. In this study, we screened for novel regulators of secondary metabolites synthesized by a clinical isolate of S. marcescens and found mutations in a gene for an uncharacterized UmoB/IgaA family member here named gumB Mutation of gumB conferred a severe loss of the secondary metabolites prodigiosin and serratamolide...
March 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29297821/isolation-identification-and-genome-analysis-of-lung-pathogenic-klebsiella-pneumoniae-lpkp-in-forest-musk-deer
#6
Wei Zhao, Qing Tian, Yan Luo, Yin Wang, Ze-Xiao Yang, Xue-Ping Yao, Jian-Guo Cheng, Xin Zhou, Wu-You Wang
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis. Median lethal dose and histopathologic analysis were used to demonstrate pathogenicity of the organism in mice. Furthermore, a draft genome of LPKP was sequenced, and its virulence genes were detected. One hundred and twenty-two virulence genes encoded determinant of capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, and urease...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29273737/hydrolytic-activity-determination-of-tail-tubular-protein-a-of-klebsiella-pneumoniae-bacteriophages-towards-saccharide-substrates
#7
Ewa Brzozowska, Anna Pyra, Krzysztof Pawlik, Monika Janik, Sabina Górska, Natalia Urbańska, Zuzanna Drulis-Kawa, Andrzej Gamian
In this paper, the enzymatic activity, substrate specificity and antibiofilm feature of bacteriophage dual-function tail proteins are presented. So far, tail tubular proteins A-TTPAgp31 and TTPAgp44-have been considered as structural proteins of Klebsiella pneumoniae bacteriophages KP32 and KP34, respectively. Our results show that TTPAgp31 is able to hydrolyze maltose as well as Red-starch. The activity of 1 µM of the protein was calculated as 47.6 milli-Units/assay relating to the α-amylase activity. It degrades capsular polysaccharides (cPS), slime polysaccharides (sPS) and lipopolysaccharide (LPS) of K...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28988127/comparative-genome-analysis-of-novel-podoviruses-lytic-for-hypermucoviscous-klebsiella-pneumoniae-of-k1-k2-and-k57-capsular-types
#8
COMPARATIVE STUDY
Ekaterina V Solovieva, Vera P Myakinina, Angelina A Kislichkina, Valentina M Krasilnikova, Vladimir V Verevkin, Vladimir V Mochalov, Anastasia I Lev, Nadezhda K Fursova, Nikolay V Volozhantsev
Hypermucoviscous (HV) strains of capsular types K1, K2 and K57 are the most virulent representatives of the Klebsiella pneumoniae species. Eight novel bacteriophages lytic for HV K. pneumoniae were isolated and characterized. Three bacteriophages, KpV41, KpV475, and KpV71 were found to have a lytic activity against mainly K. pneumoniae of capsular type K1. Two phages, KpV74, and KpV763 were lytic for K2 capsular type K. pneumoniae, and the phage KpV767 was specific to K57-type K. pneumoniae only. Two more phages, KpV766, and KpV48 had no capsular specificity...
January 2, 2018: Virus Research
https://www.readbyqxmd.com/read/28815890/a-semi-synthetic-glycoconjugate-vaccine-candidate-for-carbapenem-resistant-klebsiella-pneumoniae
#9
Peter H Seeberger, Claney L Pereira, Naeem Khan, Guozhi Xiao, Elizabeth Diago-Navarro, Katrin Reppe, Bastian Opitz, Bettina C Fries, Martin Witzenrath
Hospital-acquired infections are an increasingly serious health concern. Infections caused by carpabenem-resistant Klebsiella pneumoniae (CR-Kp) are especially problematic, with a 50 % average survival rate. CR-Kp are isolated from patients with ever greater frequency, 7 % within the EU but 62 % in Greece. At a time when antibiotics are becoming less effective, no vaccines to protect from this severe bacterial infection exist. Herein, we describe the convergent [3+3] synthesis of the hexasaccharide repeating unit from its capsular polysaccharide and related sequences...
November 6, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28729207/first-two-cases-of-severe-multifocal-infections-caused-by-klebsiella-pneumoniae-in-switzerland-characterization-of-an-atypical-non-k1-k2-serotype-strain-causing-liver-abscess-and-endocarditis
#10
Baharak Babouee Flury, Valentina Donà, Niccolò Buetti, Hansjakob Furrer, Andrea Endimiani
BACKGROUND: We describe the first two multifocal invasive infections due to Klebsiella pneumoniae recently observed in Switzerland. METHODS: Phenotypic (MIC assays and string test) and molecular analyses (PCR/Sequencing for bla, virulence factor genes and whole genome sequencing for one strain) were performed to characterize the causative K. pneumoniae isolates. RESULTS: Both K. pneumoniae isolates (Kp1 and Kp2) were pan-susceptible to antibiotics and produced narrow-spectrum SHV β-lactamases...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28716362/phosphoenolpyruvate-phosphotransferase-system-components-positively-regulate-klebsiella-biofilm-formation
#11
Yu-Tze Horng, Chi-Jen Wang, Wen-Ting Chung, Huei-Jen Chao, Yih-Yuan Chen, Po-Chi Soo
BACKGROUND/PURPOSE: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates diverse processes of bacterial physiology...
June 29, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28693930/the-prevalence-of-rectal-carriage-of-klebsiella-pneumoniae-amongst-diabetic-patients-and-their-clinical-relevance-in-taiwan-a-five-year-prospective-study
#12
Yu-Tsung Huang, Ju-Ying Jiang, Meng-Shiuan Hsu, Hsin-Sui Hsu, Chun-Hsing Liao, Po-Ren Hsueh
BACKGROUND/PURPOSE: Pyogenic liver abscess (PLA) and bacteremia caused by Klebsiella pneumoniae is a common complication among patients with diabetes mellitus (DM). The aim of this study is to investigate the prevalence of rectal carriage and serotype distribution of K. pneumoniae amongst DM patients and their clinical relevance. METHODS: We prospectively collected rectal swabs for K. pneumoniae culture in asymptomatic DM patients from March 2008 to June 2009. Seven capsular serotypes that were commonly associated with PLA were determined by capsular polysaccharide synthesis (cps) genotyping...
June 29, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28676686/two-t7-like-bacteriophages-k5-2-and-k5-4-each-encodes-two-capsule-depolymerases-isolation-and-functional-characterization
#13
Pei-Fang Hsieh, Hsiao-Hsuan Lin, Tzu-Lung Lin, Yi-Yin Chen, Jin-Town Wang
Two Klebsiella bacteriophages K5-2 and K5-4, which are able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains, were isolated and characterized. Each phage contained two open reading frames (ORFs), which encoded two putative capsule depolymerases, respectively. The first ORF encoded tail fiber proteins, which have K30/K69 depolymerase and K8 depolymerase activities. The second ORF encoded hypothetical proteins, which are almost identical in amino acid sequences, and have K5 depolymerase activity...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28617330/identification-of-pathogenic-factors-in-klebsiella-pneumoniae-using-impedimetric-sensor-equipped-with-biomimetic-surfaces
#14
Duyen Thi Ngoc Huynh, Ah-Young Kim, Young-Rok Kim
K. pneumoniae is an opportunistic pathogen that causes nosocomial infections, such as, pneumonia, urinary tract infections, septic shock, and gastro intestinal disease. Lipopolysaccharide (LPS), capsular polysaccharide, and fimbriae are recognized major virulence factors of K. pneumoniae and play key roles during early stages of infections. In this study, we functionalized the surface of gold electrode with mannose and mucin to monitor the adhesion-associated virulence factors of K. pneumoniae . The binding characteristics of K...
June 15, 2017: Sensors
https://www.readbyqxmd.com/read/28599670/whole-genome-sequencing-of-an-expec-that-caused-fatal-pneumonia-at-a-pig-farm-in-changchun-china
#15
Ling-Cong Kong, Xia Guo, Zi Wang, Yun-Hang Gao, Bo-Yan Jia, Shu-Ming Liu, Hong-Xia Ma
BACKGROUND: In recent years, highly frequent swine respiratory diseases have been caused by extraintestinal pathogenic Escherichia coli (ExPEC) in China. Due to this increase in ExPECs, this bacterial pathogen has become a threat to the development of the Chinese swine industry. To investigate ExPEC pathogenesis, we isolated a strain (named SLPE) from lesioned porcine lungs from Changchun in China, reported the draft genome and performed comparative genomic analyses. RESULTS: Based on the gross post-mortem examination, bacterial isolation, animal regression test and 16S rRNA gene sequence analysis, the pathogenic bacteria was identified as an ExPEC...
June 9, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28586386/differential-th17-response-induced-by-the-two-clades-of-the-pandemic-st258-klebsiella-pneumoniae-clonal-lineages-producing-kpc-type-carbapenemase
#16
Ann Maria Clemente, Giuseppe Castronovo, Alberto Antonelli, Marco Maria D'Andrea, Michele Tanturli, Eloisa Perissi, Sara Paccosi, Astrid Parenti, Federico Cozzolino, Gian Maria Rossolini, Maria Gabriella Torcia
The spread of KPC-type carbapenemases is mainly attributed to the global dissemination of Klebsiella pneumoniae (KP) strains belonging to the clonal group (CG) 258, including sequence type (ST) 258 and other related STs. Two distinct clades of CG258-KP have evolved, which differ mainly for the composition of their capsular polysaccharides, and recent studies indicate that clade 1 evolved from an ancestor of clade 2 by recombination of a genomic fragment carrying the capsular polysaccharide (cps) locus. In this paper, we investigated the ability of two ST258-KP strains, KKBO-1 and KK207-1, selected as representatives of ST258-KP clade 2 and clade 1, respectively, to activate an adaptive immune response using ex vivo-stimulation of PBMC from normal donors as an experimental model...
2017: PloS One
https://www.readbyqxmd.com/read/28533535/tail-tubular-protein-a-a-dual-function-tail-protein-of-klebsiella-pneumoniae-bacteriophage-kp32
#17
Anna Pyra, Ewa Brzozowska, Krzysztof Pawlik, Andrzej Gamian, Miroslawa Dauter, Zbigniew Dauter
Tail tubular protein A (TTPA) is a structural tail protein of Klebsiella pneumoniae bacteriophage KP32, and is responsible for adhering the bacteriophage to host cells. For the first time, we found that TTPA also exhibits lytic activity towards capsular exopolysaccharide (EPS) of the multiresistant clinical strain of Klebsiella pneumoniae, PCM2713, and thus should be regarded as a dual-function macromolecule that exhibits both structural and enzymatic actions. Here, we present our crystallographic and enzymatic studies of TTPA...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28437231/capsular-polysaccharide-types-and-virulence-related-traits-of-epidemic-kpc-producing-klebsiella-pneumoniae-isolates-in-a-chinese-university-hospital
#18
Yang Liu, Pan-Pan Liu, Lian-Hui Wang, Dan-Dan Wei, La-Gen Wan, Wei Zhang
Klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of blaKPC carrying K. pneumoniae (KPC-Kp) is rapidly increasing. Capsule is an important virulence factor in K. pneumoniae. In this study, we determined to first systematically characterize capsular polysaccharide (CPS) and virulence traits in KPC-Kp strains. A total of 56 KPC-Kp isolates were recovered from clinical samples in a Chinese hospital, which were assigned to clonal lineages by multilocus sequence typing (MLST)...
October 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28334288/genome-sequencing-and-comparative-analysis-of-three-hypermucoviscous-klebsiella-pneumoniae-strains-isolated-in-russia
#19
COMPARATIVE STUDY
Angelina A Kislichkina, Anastasia I Lev, Ekaterina V Komisarova, Nadezhda K Fursova, Vera P Myakinina, Tatiana N Mukhina, Alexander A Bogun, Nikolay V Volozhantsev
The prevalence and characteristics of hypermucoviscous (HV) strains among Klebsiella pneumoniae isolated in Russian hospitals were investigated. The HV strains accounted for 11% of the K. pneumoniae isolates collected in the period from 2011 to 2016, and were characterized as belonging to the K1, K2, K20 and K57 serotypes. Whole genome sequences (WGSs) of K. pneumoniae HV clinical strains KPi261 (SCPM-O-B-7850) and KPB4010 (SCPM-O-B-7846) belonging to the K1 and K2 capsular types, as well as WGSs of K. pneumoniae strain KPM9 (SCPM-O-B-7749) of the K20 capsular type isolated from freshwater, were completed...
June 1, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28132768/bacteremia-and-other-body-site-infection-caused-by-hypervirulent-and-classic-klebsiella-pneumoniae
#20
Hua Wu, Dongdong Li, Haijian Zhou, Yunfang Sun, Ling Guo, Dingxia Shen
OBJECTIVES: To investigate bacteremia and other body site infection caused by hypervirulent Klebsiella pneumoniae (hvKP), a recently recognized pathogen of invasive infection, and classic Klebsiella pneumoniae (cKP), a very common organism associated with many kinds of nosocomial infection. METHODS: Clinical information obtained from patients with both bacteremia and other body site infections caused by hvKP and/or cKP was retrospectively reviewed. Homo-hvKP (or homo-cKP) was defined as homologous hvKP (or cKP) strains from different body sites in each individual patient according to string test, virulence gene amplification and PFGE pattern...
March 2017: Microbial Pathogenesis
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