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impression cytology

Valli Priyaa, Debasis Gochhait, Durga Devi, Sathiya Devi, Neelaiah Siddaraju, Ramanitharan Manikandan
A 41 year old infertile male presented to the surgery OPD with scrotal enlargement. On clinical evaluation he had a nodular painless enlargement of the right testis towards the upper pole. In view of the clinical history of infertility and painless swelling the clinical impression was epididymitis/epididymo-orchitis possibly of tubercular etiology. This article is protected by copyright. All rights reserved.
June 4, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
Yi Qin, Yingnan Zhang, Qingfeng Liang, Xiaolin Xu, Qian Li, Zhiqiang Pan, Antoine Labbé
Purpose: To evaluate the effectiveness of autologous labial salivary gland with labial mucous membrane graft in a rhesus monkey model with severe dry eye. Methods: Eight eyes of eight rhesus monkeys with severe dry eye were included. Four eyes underwent autologous labial salivary gland and mucous membrane graft (group 1) and four eyes served as controls (group 2). The ocular surface was evaluated before and after transplantation surgery (at 1, 4, 8, 12, and 24 weeks)...
May 1, 2018: Investigative Ophthalmology & Visual Science
Waleed M Alghamdi, Maria Markoulli, Eric B Papas
SIGNIFICANCE: This cross-sectional study presented a link between contact lens wear and changes on the cellular morphology characteristics of the lid wiper (LW) epithelium, which was not visible by LW staining. PURPOSE: The aim of this study was to establish if the duration of contact lens (CL) wear affects the cellular morphology of the LW epithelium. METHODS: This was a cross-sectional study of 100 individuals with different exposures to CL wear: short, moderate, and long experience of CL wear; previous CL wearers; and nonwearers (NWs) as controls...
May 21, 2018: Optometry and Vision Science: Official Publication of the American Academy of Optometry
Pierre Nicolle, Hong Liang, Elodie Reboussin, Ghislaine Rabut, Elise Warcoin, Françoise Brignole-Baudouin, Stéphane Melik-Parsadaniantz, Christophe Baudouin, Antoine Labbe, Annabelle Reaux-Le Goazigo
Dry eye symptoms are among the leading complaints in ophthalmology. Dry eye disease (DED) is associated with significant pain affecting quality of life. Cellular and molecular mechanisms underlying ocular pain associated with DED are not fully understood. In this study, we investigated the ocular surface of patients with DED using in vivo confocal microscopy (IVCM) to quantify corneal nerve density and its relation with corneal inflammation. Gene expression of the proinflammatory markers HLA-DR, IL-6, CXCL12, and CCL2 and the receptors CXCR4 and CCR2, as well as PENK (enkephalin precursor), was therefore quantified in conjunctival impression cytology specimens...
April 17, 2018: International Journal of Molecular Sciences
Suzanne Hagan, Matthew C T Fyfe, Boatemaa Ofori-Frimpong, Katherine Oliver, Martyn R Foster, Sameer Sirohi, Yemisi Solanke, Michael Doughty, Adele Rowley, Mike Taylor, Steve Webber, Claire A Walshe
Purpose: The purpose of this study is to determine the potential of narrow spectrum kinase inhibitors (NSKIs) to treat inflammatory eye disorders. Methods: Human conjunctival epithelial (HCE) cells were retrieved from subjects via impression cytology. Real-time quantitative PCR (qPCR) was performed on HCE cells to determine gene expression of NSKI kinase targets and proinflammatory cytokines in dry eye disease (DED) patients versus healthy controls. qPCR also assessed p38α expression in hyperosmolar-treated Chang conjunctival epithelial cells...
March 1, 2018: Investigative Ophthalmology & Visual Science
Amanda García Tirado, Ana Boto de Los Bueis, Luis Rivas Jara
INTRODUCTION: To investigate the ocular surface changes occurring in eyes with recurrent pterygium post-operatively treated with 5-fluorouracil intralesional injections. METHODS: Retrospective observational study of recurrent pterygium cases treated with weekly intralesional injections of 0.1 mL (5 mg) of 5-fluorouracil (10 injections). Impression cytology samples taken from the lesion, the healthy conjunctivae (inferior, superior, and contralateral to injury), and the cornea before and after treatment were analyzed...
March 1, 2018: European Journal of Ophthalmology
Huang Guannan, Su Long, Hua Xia, Wang Dong, Zhao Shaozhen
OBJECTIVE: To assess the clinical characteristics and changes in ocular surface cytology of dry eye in patients with systemic autoimmune disease. METHODS: The case-control study was conducted in the Second Hospital of Tianjin Medical University, Tianjin, China, from February 2016 to January 2017, and comprised systemic autoimmune disease patients and healthy controls. Schirmer's I test, tear breakup time test, and fluorescein staining were performed on all subjects...
March 2018: JPMA. the Journal of the Pakistan Medical Association
Yu Meng Wang, Tsz Kin Ng, Kwong Wai Choy, Hoi Kin Wong, Wai Kit Chu, Chi Pui Pang, Vishal Jhanji
PURPOSE: To illustrate the histopathology of keratoconic corneal epithelia and its micro-ribonucleic acid (miRNA) regulation. METHODS: Corneal epithelia were collected from 27 patients with keratoconus and 26 normal patients after surgery or by impression cytology. The miRNA profile was determined using miRNA microarray. The biological roles of miRNA target genes were delineated by gene ontology and pathway analyses. The expressions of significant miRNAs were validated using TaqMan polymerase chain reaction (PCR), whereas protein localization and expression of the miRNA target genes were examined by immunofluorescence and immunoblotting analyses...
March 1, 2018: Journal of Refractive Surgery
Meng-Chen Tsai, Chun-Chi Kuo, Ying-Zhen Su, Yen-Chuan Hsieh, Shih-Sung Chuang
Effusion-based lymphoma (EBL) is a rare but distinct entity of large B-cell lymphoma in effusion without association with human herpes virus-8 (HHV-8). Spontaneous regression after pleurocentesis has been observed; but to our knowledge, there are no reports on the morphological and molecular features of subsequent aspirations in regressing cases. Here, we report the case of a 92-year-old male with chronic obstructive pulmonary disease, who presented with right pleural effusion. He had no human immunodeficiency virus, hepatitis B virus, or hepatitis C virus infection, and CT scans revealed no mass lesion...
March 8, 2018: Diagnostic Cytopathology
Christina Founta, Emmanouil Papagiannakis, Nithya Ratnavelu, Arlene Feusi, Stavros Natsis, Melissa Bradbury, Ann Fisher, Raj Naik
After treatment for cervical intraepithelial neoplasia (CIN), in the UK women who are cytology-negative, high-risk (HR) human papilloma virus (HPV) positive are referred to colposcopy. This pilot study assessed the incidence of residual/recurrent CIN and the diagnostic accuracy of colposcopy with dynamic spectral imaging (DSI) mapping in their detection.This was a prospective service evaluation carried out in a UK National Health Service (NHS) colposcopy clinic. All women, referred with negative cytology/HR-HPV positive result following treatment for CIN from March 2013 until November 2014, who were examined with the DSI digital colposcope were included...
January 2018: Medicine (Baltimore)
Frans Eberth Costa Andrade, Mab Pereira Corrêa, Alexandre Dantas Gimenes, Myrna Serapião Dos Santos, Mauro Campos, Roger Chammas, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate galectin-3 (Gal-3), a β-galactoside binding protein, as a possible biomarker in ocular allergy and further investigated the role of endogenous Gal-3 in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). METHODS: Conjunctival impression cytology specimens from control and patients with severe vernal keratoconjunctivitis, treated or untreated, were used to evaluate Gal-3 expression by immunocytochemistry. To investigate the mechanism of action of Gal-3, OVA-immunised BALB/c male wild-type (WT) and Gal-3 null (Gal-3-/- ) mice were challenged with eye drops containing OVA on days 14-16 with a subset of animals pretreated with 0...
March 3, 2018: British Journal of Ophthalmology
Tatjana Rotim, Branka Kristek, Tajana Turk, Domagoj Kretić, Marija Perić, Ivo Pušeljić, Tijana Pandurović, Mario Štefanić
The aim of the study was to assess diagnostic value and utility of selected morphological features in predicting lymph node (LN) malignancy using B-mode, Doppler ultrasonography and multivariate settings in a tertiary radiological referral center. The study included 123 patients having undergone ultrasound-guided fine-needle aspiration and cytologic analysis (FNAC) of cervical, axillary and inguinal LNs. Each LN was characterized by long/L and short/T-axis, shape, margins, echogenicity, cortical thickness, vascularization, and examiner's subjective impression...
September 2017: Acta Clinica Croatica
Nicolas Wentzensen, Joan Walker, Katie Smith, Michael A Gold, Rosemary Zuna, L Stewart Massad, Angela Liu, Michelle I Silver, S Terence Dunn, Mark Schiffman
BACKGROUND: Sensitivity for detection of precancers at colposcopy and reassurance provided by a negative colposcopy are in need of systematic study and improvement. OBJECTIVE: We sought to evaluate whether selecting the appropriate women for multiple targeted cervical biopsies based on screening cytology, human papillomavirus testing, and colposcopic impression could improve accuracy and efficiency of cervical precancer detection. STUDY DESIGN: In all, 690 women aged 18-67 years referred to colposcopy subsequent to abnormal cervical cancer screening results were included in the study (ClinicalTrials...
February 17, 2018: American Journal of Obstetrics and Gynecology
William O Iverson, Subramanya Karanth, Angela Wilcox, Cau D Pham, Shawn R Lockhart, Simone M Nicholson
A sexually mature Chinese-origin female Macaca fascicularis assigned to the high-dose group in a 26-week toxicology study with an experimental immunomodulatory therapeutic antibody (a CD40 L antagonist fusion protein) was euthanized at the scheduled terminal sacrifice on study day 192. The animal was healthy at study initiation and remained clinically normal throughout the study. On study day 141, abnormal clinical pathology changes were found during a scheduled evaluation; splenomegaly was detected on study day 149 and supported by ultrasound examination...
January 1, 2018: Veterinary Pathology
Frans Eberth Costa Andrade, Joyce Luciana Covre, Lisandra Ramos, Rossen Mihaylov Hazarbassanov, Myrna Serapião Dos Santos, Mauro Campos, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate the expression of β-galactoside-binding proteins galectin (Gal)-1 and Gal-3 in patients with keratoconus (KC) and postcorneal collagen cross-linking (CXL) treatment in vitro. METHODS: Tear fluid, cornea samples and conjunctival impression cytology specimens from control and KC patients were used to evaluate Gal-1 and Gal-3 expressions. Primary keratocytes were isolated by collagenase digestion from surgically removed corneas of five normal or KC human corneal buttons and cultured in Dulbecco's modified eagle medium/Ham's F12 medium supplemented with 2% fetal bovine serum...
May 2018: British Journal of Ophthalmology
Gustavo S Figueiredo, Borja Salvador-Culla, Oliver J Baylis, Hardeep S Mudhar, Majlinda Lako, Francisco C Figueiredo
The purpose of this study is to investigate the outcomes of penetrating keratoplasty (PKP) following autologous cultivated limbal epithelial stem cell transplantation (CLET). A prospective, single center, interventional cohort study investigating patients with unilateral total limbal stem cell deficiency (LSCD) treated with CLET who underwent PKP. Patients with confirmed corneal re-epithelialization > 6 months post-CLET, and with best-corrected visual acuity (BCVA) <0.3 logMAR were offered PKP. CLET survival assessed by slit lamp, corneal impression cytology (CIC), and in vivo confocal microscopy...
February 13, 2018: Stem Cells
Eleftheria Lili, Kimon Chatzistamatiou, Andromachi Kalpaktsidou-Vakiani, Theodoros Moysiadis, Theodoros Agorastos
The aim of the present cohort study was to assess the long-term (follow-up period up to 22 years) recurrence rate of preinvasive disease and the newly detected invasive disease rate in a cohort of women treated with excisional methods for high-grade cervical intraepithelial neoplasia (CIN).Women treated with large loop excision of the transformation zone (LLETZ) and cold knife conization (CKC) for histologically proven high-grade CIN were followed up for up to 22 years. Surgical specimens underwent histological examination and the status of endocervical as well as ectocervical margins was recorded...
January 2018: Medicine (Baltimore)
Shubhasini A R, Praveen B N, Usha Hegde, Uma K, Shubha G, Keerthi G, Shiladitya Sil
Background: Oral potentially malignant disorders (OPMDs) are lesions from which malignancy is more likely to develop that from other tissues. The potential for malignant transformation of OPMDs is estimated by determining the degree of dysplastic changes in the epithelium. Dysplasia grading has been criticized for lack of reproducibility and poor predictive value but is still considered the gold standard for diagnosing OPMDs. Since grading of dysplasia is based on architectural and cytological changes, there can be considerable inter- and intra-observer variability due to subjective impressions...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
Cansu Yuksel Elgin, Guzin Iskeleli, Ovgu Aydin
PURPOSE: To investigate changes in tear and ocular surface of patients with keratoconus using rigid gas permeable contact lenses (RGPCL) and compare them against keratoconus patients who were not using lenses as well as a control group of healthy subjects. METHODS: 24 keratoconus patients using RGPCL (Group 1) 22 patients who were not using lenses (Group 3) and 21 healthy subjects (Group 3) were included in the study. Subjective complaints about the subjects' eyes have been investigated using the ocular-surface disease index (OSDI)...
December 6, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
Michael J Doughty
PURPOSE: To assess the impact of using different microscope magnifications for the goblet cell density (GCD) estimates from conjunctival impression cytology (CIC) samples from healthy individuals METHODS: In a prospective study, CIC specimens were collected from the superior bulbar conjunctiva (12 o'clock, 5mm from limbus) of 20 adult subjects (average age 22 years) onto Millicell-CM membranes and Giemsa stained. A region from each CIC filter containing reasonably high numbers of goblet cells was imaged by light microscopy at a final magnification of 400X and then the same region assessed at 200X and then 100X...
December 4, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
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