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impression cytology

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https://www.readbyqxmd.com/read/27911424/-effectiveness-of-soft-contact-lenses-in-severe-keratoconjunctivitis-sicca-treated-with-local-immunosuppressive-therapy
#1
T N Safonova, O V Gladkova, V I Boev, A A Fedorov
AIM: To study the effectiveness of soft contact lenses in severe keratoconjunctivitis sicca (KCS) treated with local immunosuppressive therapy. MATERIAL AND METHODS: We have examined 41 patients (60 eyes) with severe KCS, including 3 men (4 eyes) and 38 women (56 eyes) aged from 27 to 76 years. Group 1 consisted of 22 persons (30 eyes) who were prescribed cyclosporine А 0.05% instillations 2 times daily, artificial tears, and soft contact lenses. Group 2 consisted of 19 patients (30 eyes) who received instillations of cyclosporine А 0...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27862219/impression-cytology-as-diagnostic-tool-in-horses-with-and-without-ocular-surface-disease
#2
Barbara K Braus, Birgit Lehenauer, Alexander Tichy, Barbara Nell, Ilse Schwendenwein
BACKGROUND: Cytology plays a major role in the diagnosis of ocular surface diseases. OBJECTIVE: To compare two cytologic sampling methods for obtaining corneal and conjunctival cell samples regarding irritation for the patient, invasiveness, sample quality and diagnostic equivalence. STUDY DESIGN: Observational prospective study. METHODS: In 5 healthy horses, conjunctival and corneal samples were taken bilaterally by ICS (impression cytology sampling) and CBS (cytobrush sampling)...
November 12, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#3
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27849395/giemsa-based-cytological-assessment-of-area-shape-and-nucleus-cytoplasm-ratio-of-goblet-cells-of-rabbit-bulbar-conjunctiva
#4
M J Doughty
Goblet cells were visualized in impression cytology specimens from bulbar conjunctiva of the rabbit eye using Giemsa staining. Highly magnified images were used to generate outlines of the goblet cells and their characteristic eccentric nuclei. Using sets of 10 cells from 15 cytology specimens, I found that the longest dimension of the goblet cells averaged 16.7 ± 2.3 μm, the shortest dimension averaged 14.4 ± 1.8 μm and the nucleus averaged 6.3 ± 0.8 μm. The goblet cells were ellipsoid in shape and the longest:shortest cell dimension ratio averaged 1...
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27816739/punch-biopsy-guided-by-both-colposcopy-and-hr-hpv-status-is-more-efficient-for-identification-of-immediate-high-grade-squamous-intraepithelial-lesion-or-worse-among-hpv-infected-women-with-atypical-squamous-cells-of-undetermined-significance
#5
Z Ding, Y Li, A Chen, M Song, Y Zhang
OBJECTIVES: To investigate the accuracy of colposcopy for diagnosing high-grade squamous intraepithelial lesion (HSIL) or worse (HSIL+) in human papillomavirus (HPV)-infected patients with atypical squamous cells of undetermined significance (ASCUS) cytology, and determine whether genotyping and viral load quantitation can be useful for detecting immediate HSIL+ risk in these patients. STUDY DESIGN: This study included 620 cases with ASCUS and positive for high-risk (HR)-HPV within 1 month before or after cervical cytology at Qilu Hospital between February 2013 and February 2014...
October 23, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27797641/effect-of-omega-3-fatty-acids-dietary-supplementation-on-ocular-surface-and-tear-film-in-diabetic-patients-with-dry-eye
#6
Constantine D Georgakopoulos, Olga E Makri, Dionisios Pagoulatos, Panagiotis Vasilakis, Politimi Peristeropoulou, Vasiliki Kouli, Maria I Eliopoulou, Caterina Psachoulia
OBJECTIVE: The objective of this study was to investigate the effect of dietary supplementation with omega-3 fatty acids on ocular surface and tear film in patients with type 2 diabetes suffering from dry eye. METHODS: Thirty-six patients suffering from type 2 diabetes and moderate to severe dry eye syndrome were included in the study. Patients were assigned to receive omega-3 long-chain polyunsaturated fatty acids for 3 months. Tear film break-up time test, Schirmer-I test, and conjunctival impression cytology analysis were performed on all patients at baseline and after 1 and 3 months...
October 31, 2016: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/27761149/endoscopic-ultrasound-guided-fine-needle-aspiration-cytology-of-metastatic-renal-cell-carcinoma-to-the-pancreas-a-multi-center-experience
#7
Rahul Pannala, Karyn M Hallberg-Wallace, Amber L Smith, Aziza Nassar, Jun Zhang, Matthew Zarka, Jordan P Reynolds, Longwen Chen
INTRODUCTION: The increasing use of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) cytology to examine pancreatic neoplasms has led to an increase in the diagnosis of metastases to the pancreas. Renal cell carcinoma (RCC) is the most common metastasis to the pancreas. Our study examines 33 cases of metastatic RCC to the pancreas sampled by EUS-FNA from four large tertiary care hospitals. MATERIALS AND METHODS: We searched the cytopathology database for RCC metastatic to the pancreas diagnosed by EUS-FNA from January 2005 to January 2015...
2016: CytoJournal
https://www.readbyqxmd.com/read/27760642/-attention-to-the-impression-cytology-in-the-evaluation-of-ocular-surface-tumors
#8
B Li, Q F Liang
Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye. It provides a precise location of the area being studied and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27760272/conjunctival-neutrophils-predict-progressive-scarring-in-ocular-mucous-membrane-pemphigoid
#9
Geraint P Williams, Peter Nightingale, Sue Southworth, Alastair K O Denniston, Paul J Tomlins, Stephen Turner, John Hamburger, Simon J Bowman, S John Curnow, Saaeha Rauz
Purpose: Ocular mucous membrane pemphigoid (OcMMP) is a rare autoimmune disorder resulting in progressive conjunctival fibrosis and ocular surface failure leading to sight loss in up to 50%. This study was designed to optimize an ocular surface sampling technique for identification of novel biomarkers associated with disease activity and/or progressive fibrosis. Methods: Fifty-seven patients with OcMMP underwent detailed examination of conjunctival inflammation and fibrosis using fornix depth measurement...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27760065/conjunctival-cytological-alterations-in-ocular-beh%C3%A3-et-disease
#10
Elif Demircan, Mehmet Citirik, Nilufer Berker, Hatice Unverdi, Sema Hucumenoglu
PURPOSE: To evaluate the ocular surface alterations and tear film functions in patients with ocular Behçet disease (BD). METHODS: This study included 48 eyes of 48 patients with inactive BD and a control group of 33 age- and sex-matched subjects. Schirmer I and tear break-up time (TBUT) tests were used to evaluate the tear film of the subjects. Impression cytology was used to evaluate morphological changes of the conjunctival surface epithelial cells. RESULTS: The mean Schirmer I test result was 11...
November 2016: Cornea
https://www.readbyqxmd.com/read/27721987/clinical-usefulness-of-monitoring-expression-levels-of-ccl24-eotaxin-2-mrna-on-the-ocular-surface-in-patients-with-vernal-keratoconjunctivitis-and-atopic-keratoconjunctivitis
#11
Yukiko Shiraki, Jun Shoji, Noriko Inada
Purpose. This study aimed to evaluate the clinical efficacy of using expression levels of CCL24 (eotaxin-2) mRNA on the ocular surface as a biomarker in patients with vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (AKC). Methods. Eighteen patients with VKC or AKC (VKC/AKC group) and 12 control subjects (control group) were enrolled in this study. The VKC/AKC clinical score was determined by objective findings in patients by using the 5-5-5 exacerbation grading scale. All subjects underwent modified impression cytology and specimens were obtained from the upper tarsal conjunctiva...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27706674/immunological-impression-cytology-of-the-conjunctival-epithelium-in-patients-with-thyroid-orbitopathy-related-dry-eye
#12
S L Hsu, P Y Lee, C H Chang, C H Chen
Thyroid orbitopathy (TO) is an autoimmune disease that is complicated by ocular surface disorders, leading to discomfort. Dry eye is very prevalent in patients with TO. Recent studies on the pathogenesis of dry eye have focused on the inflammatory process, and some supporting evidence has been discovered. Because TO is a disorder of autoimmune origin, we assumed that the association between TO and dry eye is related to inflammation. Inflammation of the ocular surface in TO-related dry eye has not been well studied...
August 30, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27584693/impression-cytology-of-the-lid-wiper-area
#13
Alex Muntz, Kevin van Doorn, Lakshman N Subbaraman, Lyndon W Jones
Few reports on the cellular anatomy of the lid wiper (LW) area of the inner eyelid exist and only one report makes use of cytological methods. The optimization of a method of collecting, staining and imaging cells from the LW region using impression cytology (IC) is described in this study. Cells are collected from the inner surface of the upper eyelid of human subjects using hydrophilic polytetrafluoroethylene (PTFE) membranes, and stained with cytological dyes to reveal the presence of goblet cells, mucins, cell nuclei and various degrees of pre- and para-keratinization...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27538195/rna-collection-from-human-conjunctival-epithelial-cells-obtained-with-a-new-device-for-impression-cytology
#14
Alberto López-Miguel, Silvia Gutiérrez-Gutiérrez, Carmen García-Vázquez, Amalia Enríquez-de-Salamanca
PURPOSE: To assess the ability of a new device (Eyeprim; Opia Technologies) designed for impression cytology (IC) to harvest RNA from conjunctival cells as compared with the conventional technique. METHODS: Cell collection was performed in both eyes using both techniques (conventional and Eyeprim) in different eyes randomized to each technique to avoid bias. The collection order was also randomized. Subjective discomfort assessment was performed using the Symptom Assessment in Dry Eye questionnaire...
January 2017: Cornea
https://www.readbyqxmd.com/read/27531295/goblet-cell-response-after-photorefractive-keratectomy-and-laser-in-situ-keratomileusis
#15
Denise S Ryan, Kraig S Bower, Rose K Sia, Marie A Shatos, Robin S Howard, Michael J Mines, Richard D Stutzman, Darlene A Dartt
PURPOSE: To determine whether patients without dry eye preoperatively have an altered conjunctival goblet cell density and mucin secretion postoperatively and to explore what factors affect changes in goblet cell density and mucin secretion. SETTING: The former Walter Reed Army Medical Center, Washington, DC, USA. DESIGN: Prospective nonrandomized clinical study. METHODS: Impression cytology was used to determine conjunctival goblet cell density before and 1 week, 1 month, and 3 months after photorefractive keratectomy (PRK) or laser in situ keratomileusis (LASIK)...
August 2016: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/27502215/spindle-cell-rhabdomyosarcoma-of-the-thumb-rare-site-rare-tumor-in-a-child
#16
Sonam Mahana, Reena Tomar, Anil Agarwal, Divya Sharma, Nita Khurana, Ruchika Gupta
Spindle-cell rhabdomyosarcoma (RMS) is a relatively recently recognized favorable variant of embryonal RMS occurring mainly in the paratesticular and head and neck regions. Cytological reports of spindle-cell RMS have been sparse in the literature and the awareness of its cytological features is not very wide among cytopathologists. A 2-year-old girl was brought to clinical attention for a progressively enlarging swelling of right thumb. Imaging studies showed it to be a soft-tissue tumor in the subcutaneous region...
December 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/27489152/interleukin-1%C3%AE-and-tumour-necrosis-factor-%C3%AE-levels-in-conjunctiva-of-diabetic-patients-with-symptomatic-moderate-dry-eye-case-control-study
#17
Chen Zhang, Lei Xi, Shaozhen Zhao, Ruihua Wei, Yue Huang, Ruibo Yang, Long Su, Xun Liu
OBJECTIVES: To compare expression of interleukin (IL)-1β and tumour necrosis factor (TNF)-α in the conjunctiva of diabetic and non-diabetic patients with symptomatic moderate dry eye. SETTING AND PARTICIPANTS: Nineteen diabetic patients with dry eye, 15 non-diabetic patients with dry eye and 14 diabetic patients without dry eye were recruited. The relative expression of IL-1β and TNF-α in conjunctival impression cytology (CIC) specimens was evaluated using immunofluorescent staining and in conjunctival biopsy specimens using immunohistochemical staining...
2016: BMJ Open
https://www.readbyqxmd.com/read/27450910/signs-and-symptoms-of-dry-eye-in-keratoconus-patients-before-and-after-intrastromal-corneal-rings-surgery
#18
Gonzalo Carracedo, Alberto Recchioni, Nicolás Alejandre-Alba, Alba Martin-Gil, Laura Batres, Ignacio Jimenez-Alfaro Morote, Jesús Pintor
PURPOSE: Based on the relationship between keratoconus and dry eye, the aim of this study was to evaluate changes in signs and symptoms of dry eye in keratoconus patients before and after intrastromal corneal ring surgery. METHODS: Fifteen keratoconus patients were enrolled in Fundación Jiménez-Díaz of Madrid and University Clinic of Optometry of the Universidad Complutense de Madrid (Madrid, Spain). Tear break up time (TBUT), Schirmer test without anesthesia, corneal staining, diadenosine tetraphosphate (Ap4A) concentration, and ocular surface disease index (OSDI) were evaluated...
July 22, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27440993/in-vivo-confocal-microscopy-of-the-sclerocorneal-limbus-after-limbal-stem-cell-transplantation-looking-for-limbal-architecture-modifications-and-cytological-phenotype-correlations
#19
Leonardo Mastropasqua, Roberta Calienno, Manuela Lanzini, Mario Nubile, Rossella Annamaria Colabelli-Gisoldi, Luca De Carlo, Augusto Pocobelli
PURPOSE: To correlate a biomicroscopic evaluation, an in vivo confocal microscopy examination, and impression cytologic findings of the corneal center and sclerocorneal limbus after cultured limbal stem cell transplantation and to test the effectiveness of in vivo confocal microscopy as a diagnostic procedure in ocular surface cell therapy reconstructive surgery. METHODS: Six eyes of six patients affected by limbal stem cell deficiency after chemical burns underwent ex vivo expanded limbal stem cell transplantation (two eyes) and ex vivo expanded limbal stem cell transplantation with subsequent penetrating keratoplasty (four eyes) to restore corneal transparency...
2016: Molecular Vision
https://www.readbyqxmd.com/read/27374469/evaluation-of-cytology-collection-techniques-and-prevalence-of-malassezia-yeast-and-bacteria-in-claw-folds-of-normal-and-allergic-dogs
#20
Kimberly L Lo, Wayne S Rosenkrantz
BACKGROUND: Canine bacterial and Malassezia paronychia are common secondary complications of atopic dermatitis and adverse food reactions. HYPOTHESIS/OBJECTIVES: The aim of this study was to compare three different sampling methods for claw fold cytology and to evaluate the numbers of bacteria, Malassezia yeast and inflammatory cells. ANIMALS: Sixty client-owned dogs were classified into three groups: (A) normal dogs; (B) allergic dogs with no clinical evidence of claw disease (brown staining, erythema, swelling, crusts or exudates); and (C) allergic dogs with clinical paronychia...
August 2016: Veterinary Dermatology
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