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impression cytology

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https://www.readbyqxmd.com/read/29223651/effects-of-the-rigid-gas-permeable-contact-lense-use-on-tear-and-ocular-surface-among-keratoconus-patients
#1
Cansu Yuksel Elgin, Guzin Iskeleli, Ovgu Aydin
PURPOSE: To investigate changes in tear and ocular surface of patients with keratoconus using rigid gas permeable contact lenses (RGPCL) and compare them against keratoconus patients who were not using lenses as well as a control group of healthy subjects. METHODS: 24 keratoconus patients using RGPCL (Group 1) 22 patients who were not using lenses (Group 3) and 21 healthy subjects (Group 3) were included in the study. Subjective complaints about the subjects' eyes have been investigated using the ocular-surface disease index (OSDI)...
December 6, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29217456/goblet-cell-density-estimate-differences-in-impression-cytology-samples-varies-with-different-magnification-of-images
#2
Michael J Doughty
PURPOSE: To assess the impact of using different microscope magnifications for the goblet cell density (GCD) estimates from conjunctival impression cytology (CIC) samples from healthy individuals METHODS: In a prospective study, CIC specimens were collected from the superior bulbar conjunctiva (12 o'clock, 5mm from limbus) of 20 adult subjects (average age 22 years) onto Millicell-CM membranes and Giemsa stained. A region from each CIC filter containing reasonably high numbers of goblet cells was imaged by light microscopy at a final magnification of 400X and then the same region assessed at 200X and then 100X...
December 4, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29193804/primary-cutaneous-aspergillosis-mimicking-malignancy-a-rare-presentation-in-an-immunocompetent-patient
#3
Rachana Chaturvedi, Ashvini Kolhe, Kirti Pardeshi, Leena Naik, Shashir Wanjare
Aspergillus species are ubiquitous opportunistic molds that cause both allergic and invasive syndromes. A 65-year-old female, farmer by occupation, presented with left upper limb and trunk swelling for one year, associated with pain, tightening of skin, and vesicles with watery discharge. Local examination showed a diffuse swelling extending from left arm to forearm and lateral chest wall associated with edema, induration, and raised temperature. The swelling was firm to hard with superficial skin ulcers and black eschar...
November 30, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29186251/histopathological-diagnosis-of-small-melanocytic-lesions-suspicious-for-malignant-melanoma
#4
Danielle Carvalho Quintella, Gabriella Campos-do-Carmo, Leonardo Pereira Quintella, Tullia Cuzzi
The concern about malignant skin neoplasms leads to the excision of smaller lesions. This study on small melanocytic lesions aims to evaluate the range of possible histopathological diagnoses, describe histopathological aspects, and assess the usefulness of serial histological sections. We performed a cross-sectional descriptive histopathological study examining 76 pigmented skin lesions up to 6 mm in diameter. Histopathological diagnoses included atypical melanocytic nevi (n=38), common melanocytic nevi (n=18), atypical lentiginous melanocytic hyperplasia with architectural features of atypical melanocytic nevi (n=7), lentigo simplex (n=2), and malignant melanoma (n=1)...
May 2017: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29182030/biomarkers-of-ocular-surface-disease-using-impression-cytology
#5
Suzanne Hagan
Impression cytology (IC) is a technique which permits the retrieval of the outermost layer of ocular surface cells via the use of various types of filters. It is a minimally invasive method of evaluating human conjunctival epithelial cell morphology in the diagnosis of dry eye disease, a common and distressing disorder associated with ageing, contact lens wear, autoimmune disorders and refractive (LASIK) surgery. IC may also be utilized in the diagnosis of other ocular diseases, such as keratoconus and thyroid orbitopathy...
November 28, 2017: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29168646/anal-cytology-histopathology-and-anoscopy-in-an-anal-dysplasia-screening-program-is-anal-cytology-enough
#6
Marco Silva, Armando Peixoto, José Alexandre Sarmento, Rosa Coelho, Guilherme Macedo
BACKGROUND AND AIM: The human papilloma virus is the leading cause of anal squamous cell carcinoma. Cytological screening may reduce the associated morbidity and mortality. The aim of the study was to estimate the agreement between anal cytological examination, histopathology and anoscopic visual impression. METHODS: A prospective study of patients who underwent anal dysplasia screening between 2011 and 2015, in a proctology clinic of a tertiary referral center...
November 23, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/29112967/expression-patterns-of-conjunctival-mucin-5ac-and-aquaporin-5-in-response-to-acute-dry-eye-stress
#7
Dhruva Bhattacharya, Li Yu, Mingwu Wang
The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC...
2017: PloS One
https://www.readbyqxmd.com/read/29112672/diagnosis-of-cervical-precancers-by-endocervical-curettage-at-colposcopy-of-women-with-abnormal-cervical-cytology
#8
Angela Hui-Chia Liu, Joan Walker, Julia C Gage, Michael A Gold, Rosemary Zuna, S Terence Dunn, Mark Schiffman, Nicolas Wentzensen
OBJECTIVE: To evaluate the performance of routine endocervical curettage (ECC) for diagnosing high-grade cervical intraepithelial neoplasia (CIN) 2 or worse and additional precancers not otherwise detected by ectocervical biopsies. METHODS: In a secondary analysis of the Biopsy Study, a cross-sectional study conducted between 2009 and 2012 at the University of Oklahoma Health and Sciences Center that found an incremental increase in detection of cervical precancers by multiple biopsies at colposcopy, ECC was performed in most women aged 30 years or older...
December 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29077953/the-comet-assay-applied-to-cells-of-the-eye
#9
Amaya Azqueta, Elisa Rundén-Pran, Elisabeth Elje, Bjørn Nicolaissen, Kristiane Haug Berg, Ingrida Smeringaiova, Katerina Jirsova, Andrew R Collins
The human eye is relatively unexplored as a source of cells for investigating DNA damage. There have been some clinical studies, using cells from surgically removed tissues, and altered DNA bases as well as strand breaks have been measured using the comet assay. Tissues examined include corneal epithelium and endothelium, lens capsule, iris and retinal pigment epithelium. For the purpose of biomonitoring for exposure to potential mutagens in the environment, the eye-relatively unprotected as it is compared with the skin-would be a valuable object for study; non-invasive techniques exist to collect lachrymal duct cells from tears, or cells from the ocular surface by impression cytology, and these methods should be further developed and validated...
October 25, 2017: Mutagenesis
https://www.readbyqxmd.com/read/29034210/biomarkers-for-response-of-melanoma-patients-to-immune-checkpoint-inhibitors-a-systematic-review
#10
Charissa A C Jessurun, Julien A M Vos, Jacqueline Limpens, Rosalie M Luiten
BACKGROUND: Immune checkpoint inhibitors (ICIs), targeting CTLA-4 or PD-1 molecules, have shown impressive therapeutic results. However, only 20-40% of advanced melanoma patients have durable responses to ICI, and these positive effects must be balanced against severe off-target immune toxicity and high costs. This urges the development of predictive biomarkers for ICI response to select patients with likely clinical benefit from treatment. Although many candidate biomarkers exist, a systematic overview of biomarkers and their usefulness is lacking...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28966835/robotic-telecytology-for-remote-cytologic-evaluation-without-an-on-site-cytotechnologist-or-cytopathologist-an-active-quality-assessment-and-experience-of-over-400-cases
#11
Sahussapont Joseph Sirintrapun, Dorota Rudomina, Allix Mazzella, Rusmir Feratovic, William Alago, Robert Siegelbaum, Oscar Lin
BACKGROUND: The first satellite center to offer interventional radiology procedures at Memorial Sloan Kettering Cancer Center opened in October 2014. Two of the procedures offered, fine needle aspirations and core biopsies, required a rapid on-site cytologic evaluation of smears and biopsy touch imprints for cellular content and adequacy. The volume and frequency of such evaluations did not justify hiring on-site cytotechnologists, and therefore, a dynamic robotic telecytology (TC) solution was created...
2017: Journal of Pathology Informatics
https://www.readbyqxmd.com/read/28954026/evaluation-of-the-ocular-surface-following-bariatric-surgery
#12
Natalia Ponte Nogueira Marques, Sérgio Felberg, Jeison Nadai de Barros, Carlos Alberto Malheiros
Purpose: To identify and evaluate changes in the ocular surface in obese patients with bariatric surgery. Methods: The study included 89 randomly selected patients; 81 (91.0%) were women, 35 were preoperative, 32 were included 0-12 months after surgery, and 22 were included >12 months after surgery. All patients completed a dry eye-specific questionnaire and were evaluated by the tear ferning test, tear-film breakup time (BUT), Schirmer's test I, examination of the ocular surface by 1% rose Bengal staining, and impression cytology...
July 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28953111/asccp-colposcopy-standards-risk-based-colposcopy-practice
#13
Nicolas Wentzensen, Mark Schiffman, Michelle I Silver, Michelle J Khan, Rebecca B Perkins, Katie M Smith, Julia C Gage, Michael A Gold, Christine Conageski, Mark H Einstein, Edward J Mayeaux, Alan G Waxman, Warner K Huh, L Stewart Massad
OBJECTIVES: The American Society for Colposcopy and Cervical Pathology (ASCCP) Colposcopy Standards recommendations address the role of and approach to colposcopy for cervical cancer prevention in the United States. MATERIALS AND METHODS: The recommendations were developed by an expert working group appointed by ASCCP's Board of Directors. This article describes the rationale, evidence, and recommendations related to risk-based colposcopy practice. RESULTS: Women referred to colposcopy have a wide range of underlying precancer risk, which can be estimated by referral screening tests including cytology and human papillomavirus testing, in conjunction with the colposcopic impression...
October 2017: Journal of Lower Genital Tract Disease
https://www.readbyqxmd.com/read/28937831/impact-of-smoking-on-the-ocular-surface-tear-function-and-tear-osmolarity
#14
Serdar Aktaş, Mehmet Tetikoğlu, Ahmet Koçak, Metin Kocacan, Hatice Aktaş, Hacı Murat Sağdık, Fatih Özcura
PURPOSE: This study evaluated the effects of cigarette smoking on the ocular surface, tear function, and tear osmolarity. MATERIALS AND METHODS: A total of 50 smokers with at least 5 years of heavy smoking (defined as 1 pack/day) and 51 nonsmoking, healthy individuals were enrolled. Tear osmolarity was measured with an osmometer (TearLab(™) Osmolarity System). Ocular surface examinations involved corneal fluorescein staining, measurement of the tear film breakup time (TBUT), the Schirmer 1 test, measurement of corneal sensitivity with a Cochet-Bonnet esthesiometer, and conjunctival impression cytology...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28858005/preinflammatory-signs-in-established-reusable-and-disposable-contact-lens-wearers
#15
Cecilia Chao, Fiona Stapleton, Mark D P Willcox, Blanka Golebiowski, Kathryn Richdale
SIGNIFICANCE: Established reusable contact lens (CL) wearers show higher tear inflammatory cytokine concentrations and greater conjunctival metaplasia in the region covered by standard soft CLs. The balance of proinflammatory to anti-inflammatory cytokines, but not individual tear cytokine concentrations, was associated with self-reported CL discomfort. PURPOSE: Daily disposable (DD) lenses are often used to improve CL discomfort, but the effect on ocular inflammatory responses has not been fully investigated...
November 2017: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/28822028/effect-of-diquafosol-tetrasodium-3-on-the-conjunctival-surface-and-clinical-findings-after-cataract-surgery-in-patients-with-dry-eye
#16
Lian Cui, Ying Li, Hyo Seok Lee, Jee Myung Yang, Won Choi, Kyung Chul Yoon
PURPOSE: To investigate the effects of diquafosol tetrasodium (DT) 3% on conjunctival impression cytologic findings in addition to clinical symptoms and signs after cataract surgery in patients with preexisting dry eye disease (DED). METHODS: Ninety-four eyes of 94 patients with DED who underwent uneventful cataract surgery were included. In total, 50 patients were treated with DT 3% (group A), while 44 patients were treated with sodium hyaluronate 0.1% (group B) postoperatively, along with topical antibiotics and steroids...
August 18, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28817168/infection-with-a-novel-derogenid-trematode-in-a-flap-necked-chameleon-chamaeleo-dilepis
#17
Nancy B Collicutt, Nicole I Stacy, Heather Stockdale Walden, April Childress, Jennifer Dill, Marita Anderson, James F X Wellehan
A 2-year-old female Flap-necked chameleon (Chamaeleo dilepis) was presented with a history of anorexia. On physical examination, three 11×4×2 mm, light tan-pink, raised, gelatinous, freely movable, poorly demarcated, raised nodules were identified within the oral cavity. An impression smear contained multiple, light blue to green structures exhibiting an 18×35 μm, elliptical-shaped anterior portion and a single 1×150 μm filament with a tapered end. Initial differential etiologies included trematode ova, flagellate protozoa, fungal sporangia, plant material, and sperm...
August 17, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28738415/monocarboxylate-transporters-mediate-fluorescein-uptake-in-corneal-epithelial-cells
#18
Yi-Chen Sun, Hau-Min Liou, Po-Ting Yeh, Wei-Li Chen, Fung-Rong Hu
Purpose: To determine the presence of monocarboxylate transporter (MCT) in human and rabbit corneal epithelium and its role in transcellular fluorescein transportation in the cornea. Methods: The presence of MCTs in human and rabbit corneal epithelium was determined by RT-PCR and immunohistochemistry. Intracellular fluorescein uptake experiment was performed using cultured human corneal epithelial cells (HCECs). The involvement of MCT in fluorescein uptake was determined by addition of MCT inhibitors to HCECs and acute dry eye model on New Zealand albino rabbits by spectrophotometry, corneal impression cytology, and external eye photographs...
July 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28715398/a-non-invasive-way-to-isolate-and-phenotype-cells-from-the-conjunctiva
#19
Tanima Bose, Aihua Hou, Ryan Lee, Louis Tong, K George Chandy
Traditionally, ocular surface cytology is studied with techniques such as spatula technology and brush technology. The problem with these techniques is that they may induce traumatic lesions on the surface of the eye, which can progress to scarring, eyelid deformity, limbal stem cell deficiency and in some cases, cause great discomfort to the subject. To avoid these clinical problems, impression cytology (IC) was developed to diagnose dry eye disease and later neoplasia, atopic disease, vernal keratoconjunctivitis and keratoconjunctivitis sicca...
July 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28701833/anaplastic-large-cell-lymphoma-a-great-mimic-on-cytology
#20
Mona A Agnihotri, Kanchan S Kothari, Leena P Naik, Sharada Patil
Anaplastic large cell lymphoma (ALCL) is a T-cell lymphoma, accounting for <5% of non-Hodgkin's lymphoma. Cutaneous involvement can be primary or secondary arising in systemic ALCL. The diagnostic feature in both is the presence of pleomorphic, CD30 positive hallmark cells. We present a case of ALCL in a 19-year-old male presenting as an ulcerated scalp swelling. Clinical impression was actinomycosis or scrofuloderma. Cytology smears showed large dispersed pleomorphic cells with hyperlobated nuclei and multinucleated giant cells...
July 2017: Journal of Cytology
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