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impression cytology

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https://www.readbyqxmd.com/read/28306352/evaluation-of-the-tear-function-tests-and-the-ocular-surface-in-first-time-users-of-silicone-hydrogel-contact-lenses
#1
Yelda Yildiz Tasci, Canan Gürdal, Ozge Sarac, Aykut Onusever
PURPOSE: To evaluate the effects of three different silicone hydrogel contact lenses (SHCL), (balafilcon A, senofilcon A, and comfilcon A) on tear function tests, corneal thickness, and ocular surface cytology in first time contact lens users. MATERIALS AND METHODS: In this prospective study, 120 eyes of 60 subjects were evaluated. Balafilcon A users were designated as group 1, senofilcon A users as group 2, and comfilcon A users as group 3. In all cases, before and after 6 months of contact lens wear, ocular surface disease index score (OSDI), tear breakup time (TBUT), Schirmer 1 test, central corneal thickness (CCT), central corneal epithelium thickness (CCET), and conjunctival impression cytology samples were evaluated...
March 17, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28304385/local-ocular-surface-parameters-in-patients-with-systemic-celiac-disease
#2
M M Uzel, M Citirik, M Kekilli, P Cicek
PurposeThis was a collaborative study from the Ophthalmology and Gastroenterology departments of a tertiary care hospital, designed to investigate the local ocular surface parameters in patients with celiac disease (CD).MethodsFifty-six eyes of 28 patients with CD and 58 eyes of 29 healthy adult subjects serving as controls were evaluated. The Schirmer test, tear-film break-up time, and conjunctival impression cytology were performed in addition to a complete ophthalmological examination. Impression cytology specimens of each group were graded and scored in the range 0-3...
March 17, 2017: Eye
https://www.readbyqxmd.com/read/28302532/randomized-controlled-crossover-trial-comparing-the-impact-of-sham-or-intranasal-neurostimulation-on-conjunctival-goblet-cell-degranulation
#3
Koray Gumus, Karri L Schuetzle, Stephen C Pflugfelder
PURPOSE: The aim of the study was to investigate the effects of the Oculeve Intranasal Lacrimal Neurostimulator (ILN) on conjunctival goblet cell degranulation. DESIGN: A randomized, double-masked, placebo-controlled crossover trial METHODS: A total of 15 subjects (5 normal and 10 dry eye) were enrolled in a three-visit study consisting of one screening and two separate randomized-masked ILN treatments (sham extranasal or intranasal). Tear meniscus height (TMH) was measured by AS-OCT before and after applications...
March 13, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28299002/impression-cytology-in-a-series-of-clinically-diagnosed-ocular-surface-melanocytic-lesions
#4
Mozhgan Rezaei Kanavi, Seyed Bagher Hosseini, Roshanak Aliakbar-Navahi, Hossein Aghaei
PURPOSE: To report impression cytology (IC) results of clinically diagnosed ocular surface melanocytic lesions. METHODS: Ten patients with a clinical diagnosis of an ocular surface melanocytic lesion underwent IC using cellulose acetate strips and Periodic acid Schiff-Papanicolaou staining. Excisional biopsy of lesions was performed in case of observing atypical cells on IC or at the patient's request, and excised specimens were subjected to histopathological analysis...
January 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28292693/influence-of-high-risk-hpv-genotype-on-colposcopic-performance-a-large-prospective-study-demonstrates-improved-detection-of-disease-with-zedscan-i-particularly-in-non-hpv-16-patients
#5
Madeleine C Macdonald, Brian H Brown, Rachel E Lyon, T Jamie Healey, Julia E Palmer, John A Tidy
OBJECTIVE: To assess the influence of high-risk Human Papilloma Virus (hrHPV) genotyping on the detection of high-grade disease (CIN2+) using colposcopic impression both with and without electrical impedance spectroscopy (ZedScan I) as an adjunct. STUDY DESIGN: A prospective cohort of women with a known hrHPV genotype referred to a single colposcopy service. RESULTS: 839 women underwent colposcopy and ZedScan I examination. 613 women were referred with abnormal cytology; 411 (67%) with low-grade dyskaryosis (67%) and 202 (33%) with high-grade dyskaryosis...
February 20, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28262005/parotid-or-carotid-misled-by-site
#6
Ruchita Tyagi, Manish Munjal, Pavneet Kaur, Harpreet Kaur, Neena Sood
We present an interesting scenario where a 64 years old male presented with a long standing painless, infra-auricular swelling, which had progressively increased in size. Based on the site, the clinical impression was of a salivary gland lesion and FNAC was performed. The smears were unusually cellular and had necrotic background. The cytological diagnosis was a cystic neoplasm of salivary gland, possibly mucoepidermoid carcinoma. Warthin's tumor was also kept in differential. However, the radiological investigations, which were made available after the FNAC report were conflicting with cytological diagnosis of a malignancy and were characteristic of a carotid body tumor, generally a benign neoplasm...
March 6, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28261993/carcinoma-ex-pleomorphic-adenoma-a-diagnostic-challenge-on-cytology
#7
Kanika Singh, Charu Agarwal, Mukta Pujani, Pragya Verma, Varsha Chauhan
Carcinoma ex pleomorphic adenoma (Ca ex PA) is a malignant neoplasm arising from primary or recurrent benign pleomorphic adenoma. It is rare with an annual incidence rate of 0.17 tumors per million. Histopathology remains the gold standard for the diagnosis of Ca ex PA, with only a handful of cases reported on cytology. In our case a 66-year-old male presented with the right parotid mass for 5 years rapidly increasing for the last 3 months. Fine needle aspiration cytology (FNAC) smears showed malignant tumor cells in clusters along with benign myoepithelial cells in chondromyxoid background...
March 6, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28251273/clinico-pathological-correlation-of-thyroid-nodule-ultrasound-and-cytology-using-the-tirads-and-bethesda-classifications
#8
R M Singaporewalla, J Hwee, T U Lang, V Desai
BACKGROUND: Clinico-pathological correlation of thyroid nodules is not routinely performed as until recently there was no objective classification system for reporting thyroid nodules on ultrasound. We compared the Thyroid Imaging Reporting and Data System (TIRADS) of classifying thyroid nodules on ultrasound with the findings on fine-needle aspiration cytology (FNAC) reported using the Bethesda System. METHODS: A retrospective analysis of 100 consecutive cases over 1 year (Jan-Dec 2015) was performed comparing single-surgeon-performed bedside thyroid nodule ultrasound findings based on the TIRADS classification to the FNAC report based on the Bethesda Classification...
March 1, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28224303/conjunctival-cytologic-features-in-patients-with-unilateral-primary-acquired-nasolacrimal-duct-obstruction
#9
Nilay Yuksel, Melek Mutlu, Aydan Kilicarslan, Emine Akcay
PURPOSE: To evaluate the cytologic features of conjunctival epithelium in patients with unilateral primary acquired nasolacrimal duct obstruction (PANDO) using impression cytology. METHODS: Twenty-four patients with unilateral PANDO for at least 1 year were enrolled in this study. The healthy eyes of the patients are assessed as control group. All patients were subjected to ophthalmic surface examination with conjunctival impression cytology analysis. Squamous metaplasia and goblet cell density were graded according to Nelson grading system...
February 21, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28198730/conjunctival-impression-cytology-and-dry-eye-in-patients-with-ulcerative-colitis-a-pilot-study
#10
Pembe Oltulu, Refik Oltulu, Mehmet Asil, Gunhal Satirtav, Enver Mirza
PURPOSE: The aim of this study was to compare signs of dry eye between ulcerative colitis (UC) patients and healthy subjects. METHODS: Fifteen patients with UC (group 1) and 15 healthy subjects (group 2) were enrolled in this study. Tear volume measurement, Schirmer-I test with no anesthetic, tear break-up time, and conjunctival impression cytology (CIC) were evaluated in both groups. RESULTS: Patients with UC showed lower tear volumes when compared with healthy subjects (P<0...
February 14, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28139361/is-intraoperative-touch-imprint-cytology-indicated-in-the-surgical-treatment-of-early-breast-cancers
#11
Z Horváth, A Paszt, Z Simonka, M Látos, V Oláh, D Nagyszegi, L Kaizer, Z Fejes, S Hamar, E Csörgő, K Ormándi, M Lázár, G Lázár
INTRODUCTION: Intraoperative touch imprint cytology (TIC) of the sentinel lymph node(s) (SLN(s)) in the treatment of breast cancer has significantly reduced the number of axillary block dissections (ABD) required during second surgeries. Based on recent studies, ABD was not considered necessary if the presence of tumor cells/micrometastasis was confirmed in the SLN(s) or in the case of macrometastases in a patient group meeting the inclusion criteria for the ACOSOG Z0011 study. Our aim was to determine the sensitivity and usefulness of TIC with regard to these results...
January 18, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28117903/cytology-of-the-healthy-canine-and-feline-ocular-surface-comparison-between-cytobrush-and-impression-technique
#12
Anna Perazzi, Federico Bonsembiante, Maria Elena Gelain, Marco Patruno, Enzo Di Iorio, Angelo Migliorati, Ilaria Iacopetti
BACKGROUND: Impression cytology (IC) is a noninvasive technique in which filters are used to sample superficial layers of ocular epithelium. OBJECTIVES: The aim of this study was to compare cytology specimens obtained by IC and cytobrush from healthy canine and feline eyes. METHODS: Dogs and cats were prospectively sampled using polytetrafluorethylene filters on the right eye, and cytobrush on the left eye. Wright-Giemsa-stained specimens were evaluated by 2 observers...
January 24, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28089075/conjunctival-impression-cytology-evaluation-of-patients-with-dry-eye-disease-using-scleral-contact-lenses
#13
Sarah La Porta Weber, Rossen Mihaylov Hazarbassanov, Alex Nasaré, José Álvaro Pereira Gomes, Ana Luisa Hofling-Lima
PURPOSE: To evaluate conjunctival impression cytology and HLADR expression changes after wearing scleral contact lenses (ScCLs) for moderate to severe dry eye disease (DED). DESIGN: Prospective interventional case series. METHODS: Forty-one eyes from 25 patients with moderate to severe DED were evaluated for Esclera ScCL treatment. Best-corrected visual acuity (BCVA) and slit-lamp findings were assessed. Impression cytology specimens were obtained from DED patients at the baseline and after wearing ScCLs for 12 months...
January 6, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28019691/biofilm-detection-by-wound-blotting-can-predict-slough-development-in-pressure-ulcers-a-prospective-observational-study
#14
Gojiro Nakagami, Gregory Schultz, Daniel J Gibson, Priscilla Phillips, Aya Kitamura, Takeo Minematsu, Tomomitsu Miyagaki, Akitatsu Hayashi, Sanae Sasaki, Junko Sugama, Hiromi Sanada
Bacteria have been found to form multicellular aggregates which have collectively been termed "biofilms." It is hypothesized that biofilm formation is a means to protect bacterial cells including protection form the immune response of humans. This protective mechanism is believed to explain persistent chronic wound infections. At times, the biofilms are abundant enough to see, and remove by simple wiping. However, recent evidence has shown that the removal of these visible portions are not sufficient, and that biofilms can continue to form even with daily wiping...
December 26, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27990362/corneal-sensitivity-ocular-surface-health-and-tear-film-stability-after-punctal-plug-therapy-of-aqueous-deficient-dry-eye
#15
Azza Mohamed Ahmed Said, Mona Elsayed Farag, Tarek Mohamed Abdulla, Othman Ali Othman Ziko, Wesam Mohamed Osman
AIM: To evaluate the effect of punctal occlusion using thermosensitive (smart plug) versus silicone plug for management of aqueous deficient dry eye on corneal sensitivity, ocular surface health and tear film stability. METHODS: A comparative prospective interventional case study included 45 patients with bilateral severe form of aqueous deficient dry eye. In each patient, the smart plug was inserted in the lower punctum of the right eye which was considered as study group 1 and silicone plug was inserted in the lower punctum of the left eye of the same patient which was considered as study group 2...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27911424/-effectiveness-of-soft-contact-lenses-in-severe-keratoconjunctivitis-sicca-treated-with-local-immunosuppressive-therapy
#16
T N Safonova, O V Gladkova, V I Boev, A A Fedorov
AIM: To study the effectiveness of soft contact lenses in severe keratoconjunctivitis sicca (KCS) treated with local immunosuppressive therapy. MATERIAL AND METHODS: We have examined 41 patients (60 eyes) with severe KCS, including 3 men (4 eyes) and 38 women (56 eyes) aged from 27 to 76 years. Group 1 consisted of 22 persons (30 eyes) who were prescribed cyclosporine А 0.05% instillations 2 times daily, artificial tears, and soft contact lenses. Group 2 consisted of 19 patients (30 eyes) who received instillations of cyclosporine А 0...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27862219/impression-cytology-as-diagnostic-tool-in-horses-with-and-without-ocular-surface-disease
#17
B K Braus, B Lehenauer, A Tichy, B Nell, I Schwendenwein
BACKGROUND: Cytology plays a major role in the diagnosis of ocular surface diseases. OBJECTIVE: To compare 2 cytological sampling methods for obtaining corneal and conjunctival cell samples regarding irritation for the patient, invasiveness, sample quality and diagnostic equivalence. STUDY DESIGN: Observational prospective study. METHODS: In 5 healthy horses, conjunctival and corneal samples were taken bilaterally by impression cytology sampling (ICS) and cytobrush sampling (CBS)...
November 12, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#18
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods used in the diagnosis of LSCD in published interventional studies. The design is a systematic literature review. We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003, and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27849395/giemsa-based-cytological-assessment-of-area-shape-and-nucleus-cytoplasm-ratio-of-goblet-cells-of-rabbit-bulbar-conjunctiva
#19
M J Doughty
Goblet cells were visualized in impression cytology specimens from bulbar conjunctiva of the rabbit eye using Giemsa staining. Highly magnified images were used to generate outlines of the goblet cells and their characteristic eccentric nuclei. Using sets of 10 cells from 15 cytology specimens, I found that the longest dimension of the goblet cells averaged 16.7 ± 2.3 μm, the shortest dimension averaged 14.4 ± 1.8 μm and the nucleus averaged 6.3 ± 0.8 μm. The goblet cells were ellipsoid in shape and the longest:shortest cell dimension ratio averaged 1...
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27816739/punch-biopsy-guided-by-both-colposcopy-and-hr-hpv-status-is-more-efficient-for-identification-of-immediate-high-grade-squamous-intraepithelial-lesion-or-worse-among-hpv-infected-women-with-atypical-squamous-cells-of-undetermined-significance
#20
Z Ding, Y Li, A Chen, M Song, Y Zhang
OBJECTIVES: To investigate the accuracy of colposcopy for diagnosing high-grade squamous intraepithelial lesion (HSIL) or worse (HSIL+) in human papillomavirus (HPV)-infected patients with atypical squamous cells of undetermined significance (ASCUS) cytology, and determine whether genotyping and viral load quantitation can be useful for detecting immediate HSIL+ risk in these patients. STUDY DESIGN: This study included 620 cases with ASCUS and positive for high-risk (HR)-HPV within 1 month before or after cervical cytology at Qilu Hospital between February 2013 and February 2014...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
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