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impression cytology

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https://www.readbyqxmd.com/read/28198730/conjunctival-impression-cytology-and-dry-eye-in-patients-with-ulcerative-colitis-a-pilot-study
#1
Pembe Oltulu, Refik Oltulu, Mehmet Asil, Gunhal Satirtav, Enver Mirza
PURPOSE: The aim of this study was to compare signs of dry eye between ulcerative colitis (UC) patients and healthy subjects. METHODS: Fifteen patients with UC (group 1) and 15 healthy subjects (group 2) were enrolled in this study. Tear volume measurement, Schirmer-I test with no anesthetic, tear break-up time, and conjunctival impression cytology (CIC) were evaluated in both groups. RESULTS: Patients with UC showed lower tear volumes when compared with healthy subjects (P<0...
February 14, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28139361/is-intraoperative-touch-imprint-cytology-indicated-in-the-surgical-treatment-of-early-breast-cancers
#2
Z Horváth, A Paszt, Z Simonka, M Látos, V Oláh, D Nagyszegi, L Kaizer, Z Fejes, S Hamar, E Csörgő, K Ormándi, M Lázár, G Lázár
INTRODUCTION: Intraoperative touch imprint cytology (TIC) of the sentinel lymph node(s) (SLN(s)) in the treatment of breast cancer has significantly reduced the number of axillary block dissections (ABD) required during second surgeries. Based on recent studies, ABD was not considered necessary if the presence of tumor cells/micrometastasis was confirmed in the SLN(s) or in the case of macrometastases in a patient group meeting the inclusion criteria for the ACOSOG Z0011 study. Our aim was to determine the sensitivity and usefulness of TIC with regard to these results...
January 18, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28117903/cytology-of-the-healthy-canine-and-feline-ocular-surface-comparison-between-cytobrush-and-impression-technique
#3
Anna Perazzi, Federico Bonsembiante, Maria Elena Gelain, Marco Patruno, Enzo Di Iorio, Angelo Migliorati, Ilaria Iacopetti
BACKGROUND: Impression cytology (IC) is a noninvasive technique in which filters are used to sample superficial layers of ocular epithelium. OBJECTIVES: The aim of this study was to compare cytology specimens obtained by IC and cytobrush from healthy canine and feline eyes. METHODS: Dogs and cats were prospectively sampled using polytetrafluorethylene filters on the right eye, and cytobrush on the left eye. Wright-Giemsa-stained specimens were evaluated by 2 observers...
January 24, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28089075/conjunctival-impression-cytology-evaluation-of-patients-with-dry-eye-disease-using-scleral-contact-lenses
#4
Sarah La Porta Weber, Rossen Mihaylov Hazarbassanov, Alex Nasaré, José Álvaro Pereira Gomes, Ana Luisa Hofling-Lima
PURPOSE: To evaluate conjunctival impression cytology and HLADR expression changes after wearing scleral contact lenses (ScCLs) for moderate to severe dry eye disease (DED). DESIGN: Prospective interventional case series. METHODS: Forty-one eyes from 25 patients with moderate to severe DED were evaluated for Esclera ScCL treatment. Best-corrected visual acuity (BCVA) and slit-lamp findings were assessed. Impression cytology specimens were obtained from DED patients at the baseline and after wearing ScCLs for 12 months...
January 6, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28019691/biofilm-detection-by-wound-blotting-can-predict-slough-development-in-pressure-ulcers-a-prospective-observational-study
#5
Gojiro Nakagami, Gregory Schultz, Daniel J Gibson, Priscilla Phillips, Aya Kitamura, Takeo Minematsu, Tomomitsu Miyagaki, Akitatsu Hayashi, Sanae Sasaki, Junko Sugama, Hiromi Sanada
Bacteria have been found to form multicellular aggregates which have collectively been termed "biofilms". It is hypothesized that biofilm formation is a means to protect bacterial cells including protection form the immune response of humans. This protective mechanism is believed to explain persistent chronic wound infections. At times, the biofilms are abundant enough to see, and remove by simple wiping. However, recent evidence has shown that the removal of these visible portions are not sufficient, and that biofilms can continue to form even with daily wiping...
December 26, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27990362/corneal-sensitivity-ocular-surface-health-and-tear-film-stability-after-punctal-plug-therapy-of-aqueous-deficient-dry-eye
#6
Azza Mohamed Ahmed Said, Mona Elsayed Farag, Tarek Mohamed Abdulla, Othman Ali Othman Ziko, Wesam Mohamed Osman
AIM: To evaluate the effect of punctal occlusion using thermosensitive (smart plug) versus silicone plug for management of aqueous deficient dry eye on corneal sensitivity, ocular surface health and tear film stability. METHODS: A comparative prospective interventional case study included 45 patients with bilateral severe form of aqueous deficient dry eye. In each patient, the smart plug was inserted in the lower punctum of the right eye which was considered as study group 1 and silicone plug was inserted in the lower punctum of the left eye of the same patient which was considered as study group 2...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27911424/-effectiveness-of-soft-contact-lenses-in-severe-keratoconjunctivitis-sicca-treated-with-local-immunosuppressive-therapy
#7
T N Safonova, O V Gladkova, V I Boev, A A Fedorov
AIM: To study the effectiveness of soft contact lenses in severe keratoconjunctivitis sicca (KCS) treated with local immunosuppressive therapy. MATERIAL AND METHODS: We have examined 41 patients (60 eyes) with severe KCS, including 3 men (4 eyes) and 38 women (56 eyes) aged from 27 to 76 years. Group 1 consisted of 22 persons (30 eyes) who were prescribed cyclosporine А 0.05% instillations 2 times daily, artificial tears, and soft contact lenses. Group 2 consisted of 19 patients (30 eyes) who received instillations of cyclosporine А 0...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27862219/impression-cytology-as-diagnostic-tool-in-horses-with-and-without-ocular-surface-disease
#8
B K Braus, B Lehenauer, A Tichy, B Nell, I Schwendenwein
BACKGROUND: Cytology plays a major role in the diagnosis of ocular surface diseases. OBJECTIVE: To compare 2 cytological sampling methods for obtaining corneal and conjunctival cell samples regarding irritation for the patient, invasiveness, sample quality and diagnostic equivalence. STUDY DESIGN: Observational prospective study. METHODS: In 5 healthy horses, conjunctival and corneal samples were taken bilaterally by impression cytology sampling (ICS) and cytobrush sampling (CBS)...
November 12, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#9
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27849395/giemsa-based-cytological-assessment-of-area-shape-and-nucleus-cytoplasm-ratio-of-goblet-cells-of-rabbit-bulbar-conjunctiva
#10
M J Doughty
Goblet cells were visualized in impression cytology specimens from bulbar conjunctiva of the rabbit eye using Giemsa staining. Highly magnified images were used to generate outlines of the goblet cells and their characteristic eccentric nuclei. Using sets of 10 cells from 15 cytology specimens, I found that the longest dimension of the goblet cells averaged 16.7 ± 2.3 μm, the shortest dimension averaged 14.4 ± 1.8 μm and the nucleus averaged 6.3 ± 0.8 μm. The goblet cells were ellipsoid in shape and the longest:shortest cell dimension ratio averaged 1...
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27816739/punch-biopsy-guided-by-both-colposcopy-and-hr-hpv-status-is-more-efficient-for-identification-of-immediate-high-grade-squamous-intraepithelial-lesion-or-worse-among-hpv-infected-women-with-atypical-squamous-cells-of-undetermined-significance
#11
Z Ding, Y Li, A Chen, M Song, Y Zhang
OBJECTIVES: To investigate the accuracy of colposcopy for diagnosing high-grade squamous intraepithelial lesion (HSIL) or worse (HSIL+) in human papillomavirus (HPV)-infected patients with atypical squamous cells of undetermined significance (ASCUS) cytology, and determine whether genotyping and viral load quantitation can be useful for detecting immediate HSIL+ risk in these patients. STUDY DESIGN: This study included 620 cases with ASCUS and positive for high-risk (HR)-HPV within 1 month before or after cervical cytology at Qilu Hospital between February 2013 and February 2014...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27797641/effect-of-omega-3-fatty-acids-dietary-supplementation-on-ocular-surface-and-tear-film-in-diabetic-patients-with-dry-eye
#12
Constantine D Georgakopoulos, Olga E Makri, Dionisios Pagoulatos, Panagiotis Vasilakis, Politimi Peristeropoulou, Vasiliki Kouli, Maria I Eliopoulou, Caterina Psachoulia
OBJECTIVE: The objective of this study was to investigate the effect of dietary supplementation with omega-3 fatty acids on ocular surface and tear film in patients with type 2 diabetes suffering from dry eye. METHODS: Thirty-six patients suffering from type 2 diabetes and moderate to severe dry eye syndrome were included in the study. Patients were assigned to receive omega-3 long-chain polyunsaturated fatty acids for 3 months. Tear film break-up time test, Schirmer-I test, and conjunctival impression cytology analysis were performed on all patients at baseline and after 1 and 3 months...
October 31, 2016: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/27761149/endoscopic-ultrasound-guided-fine-needle-aspiration-cytology-of-metastatic-renal-cell-carcinoma-to-the-pancreas-a-multi-center-experience
#13
Rahul Pannala, Karyn M Hallberg-Wallace, Amber L Smith, Aziza Nassar, Jun Zhang, Matthew Zarka, Jordan P Reynolds, Longwen Chen
INTRODUCTION: The increasing use of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) cytology to examine pancreatic neoplasms has led to an increase in the diagnosis of metastases to the pancreas. Renal cell carcinoma (RCC) is the most common metastasis to the pancreas. Our study examines 33 cases of metastatic RCC to the pancreas sampled by EUS-FNA from four large tertiary care hospitals. MATERIALS AND METHODS: We searched the cytopathology database for RCC metastatic to the pancreas diagnosed by EUS-FNA from January 2005 to January 2015...
2016: CytoJournal
https://www.readbyqxmd.com/read/27760642/-attention-to-the-impression-cytology-in-the-evaluation-of-ocular-surface-tumors
#14
B Li, Q F Liang
Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye. It provides a precise location of the area being studied and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27760272/conjunctival-neutrophils-predict-progressive-scarring-in-ocular-mucous-membrane-pemphigoid
#15
Geraint P Williams, Peter Nightingale, Sue Southworth, Alastair K O Denniston, Paul J Tomlins, Stephen Turner, John Hamburger, Simon J Bowman, S John Curnow, Saaeha Rauz
Purpose: Ocular mucous membrane pemphigoid (OcMMP) is a rare autoimmune disorder resulting in progressive conjunctival fibrosis and ocular surface failure leading to sight loss in up to 50%. This study was designed to optimize an ocular surface sampling technique for identification of novel biomarkers associated with disease activity and/or progressive fibrosis. Methods: Fifty-seven patients with OcMMP underwent detailed examination of conjunctival inflammation and fibrosis using fornix depth measurement...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27760065/conjunctival-cytological-alterations-in-ocular-beh%C3%A3-et-disease
#16
Elif Demircan, Mehmet Citirik, Nilufer Berker, Hatice Unverdi, Sema Hucumenoglu
PURPOSE: To evaluate the ocular surface alterations and tear film functions in patients with ocular Behçet disease (BD). METHODS: This study included 48 eyes of 48 patients with inactive BD and a control group of 33 age- and sex-matched subjects. Schirmer I and tear break-up time (TBUT) tests were used to evaluate the tear film of the subjects. Impression cytology was used to evaluate morphological changes of the conjunctival surface epithelial cells. RESULTS: The mean Schirmer I test result was 11...
November 2016: Cornea
https://www.readbyqxmd.com/read/27721987/clinical-usefulness-of-monitoring-expression-levels-of-ccl24-eotaxin-2-mrna-on-the-ocular-surface-in-patients-with-vernal-keratoconjunctivitis-and-atopic-keratoconjunctivitis
#17
Yukiko Shiraki, Jun Shoji, Noriko Inada
Purpose. This study aimed to evaluate the clinical efficacy of using expression levels of CCL24 (eotaxin-2) mRNA on the ocular surface as a biomarker in patients with vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (AKC). Methods. Eighteen patients with VKC or AKC (VKC/AKC group) and 12 control subjects (control group) were enrolled in this study. The VKC/AKC clinical score was determined by objective findings in patients by using the 5-5-5 exacerbation grading scale. All subjects underwent modified impression cytology and specimens were obtained from the upper tarsal conjunctiva...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27706674/immunological-impression-cytology-of-the-conjunctival-epithelium-in-patients-with-thyroid-orbitopathy-related-dry-eye
#18
S L Hsu, P Y Lee, C H Chang, C H Chen
Thyroid orbitopathy (TO) is an autoimmune disease that is complicated by ocular surface disorders, leading to discomfort. Dry eye is very prevalent in patients with TO. Recent studies on the pathogenesis of dry eye have focused on the inflammatory process, and some supporting evidence has been discovered. Because TO is a disorder of autoimmune origin, we assumed that the association between TO and dry eye is related to inflammation. Inflammation of the ocular surface in TO-related dry eye has not been well studied...
August 30, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27584693/impression-cytology-of-the-lid-wiper-area
#19
Alex Muntz, Kevin van Doorn, Lakshman N Subbaraman, Lyndon W Jones
Few reports on the cellular anatomy of the lid wiper (LW) area of the inner eyelid exist and only one report makes use of cytological methods. The optimization of a method of collecting, staining and imaging cells from the LW region using impression cytology (IC) is described in this study. Cells are collected from the inner surface of the upper eyelid of human subjects using hydrophilic polytetrafluoroethylene (PTFE) membranes, and stained with cytological dyes to reveal the presence of goblet cells, mucins, cell nuclei and various degrees of pre- and para-keratinization...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27538195/rna-collection-from-human-conjunctival-epithelial-cells-obtained-with-a-new-device-for-impression-cytology
#20
Alberto López-Miguel, Silvia Gutiérrez-Gutiérrez, Carmen García-Vázquez, Amalia Enríquez-de-Salamanca
PURPOSE: To assess the ability of a new device (Eyeprim; Opia Technologies) designed for impression cytology (IC) to harvest RNA from conjunctival cells as compared with the conventional technique. METHODS: Cell collection was performed in both eyes using both techniques (conventional and Eyeprim) in different eyes randomized to each technique to avoid bias. The collection order was also randomized. Subjective discomfort assessment was performed using the Symptom Assessment in Dry Eye questionnaire...
January 2017: Cornea
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