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https://www.readbyqxmd.com/read/29456673/full-thickness-conjunctival-flap-covering-surgery-combined-with-amniotic-membrane-transplantation-for-severe-fungal-keratitis
#1
Jing Zhong, Bowen Wang, Saiqun Li, Yuqing Deng, Haixiang Huang, Ling Chen, Jin Yuan
Fungal keratitis is a severe and common ocular infectious disease. The present study evaluated the efficiency of full-thickness conjunctival flap covering surgery with amniotic membrane transplantation (FCCS + AMT) as a transitional surgery for severe fungal keratitis. A total of 17 patients with severe fungal keratitis without corneal perforation underwent FCCS + AMT between January 2010 and December 2015. The pathogenic factors, preoperative diagnosis and postoperative prognosis of FCCS + AMT at 3 months were evaluated...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29455122/benzotriazoles-and-benzothiazoles-in-paired-maternal-urine-and-amniotic-fluid-samples-from-tianjin-china
#2
Xiaoying Li, Lei Wang, Alexandros G Asimakopoulos, Hongwen Sun, Zhen Zhao, Junjie Zhang, Lu Zhang, Qi Wang
Benzotriazoles (BTRs) and benzothiazoles (BTHs) are two groups of heterocyclic compounds that are widely detected in the environment. In this study, the levels of BTRs and BTHs in 79 paired maternal urine and amniotic fluid samples from Tianjin were investigated. BTRs were detected in most maternal urine samples, with a median concentration of ∑BTRs of 0.88 ng/mL. BTH was detected in all maternal urine samples, with a median concentration of 1.35 ng/mL. Tolyltriazole (TTR, i.e., the sum of 4-methyl-1H-benzotriazole and 5-methyl-1H-benzotriazole) and BTH were detected in amniotic fluid with detection rates (DRs) > 50% and median concentrations of 0...
February 12, 2018: Chemosphere
https://www.readbyqxmd.com/read/29452173/energy-intake-functions-and-energy-budgets-of-ectotherms-and-endotherms-derived-from-their-ontogenetic-growth-in-body-mass-and-timing-of-sexual-maturation
#3
Jan Werner, Nikolaos Sfakianakis, Alan D Rendall, Eva Maria Griebeler
Ectothermic and endothermic vertebrates differ not only in their source of body temperature (environment vs. metabolism), but also in growth patterns, in timing of sexual maturation within life, and energy intake functions. Here, we present a mathematical model applicable to ectothermic and endothermic vertebrates. It is designed to test whether differences in the timing of sexual maturation within an animal's life (age at which sexual maturity is reached vs. longevity) together with its ontogenetic gain in body mass (growth curve) can predict the energy intake throughout the animal's life (food intake curve) and can explain differences in energy partitioning (between growth, reproduction, heat production and maintenance, with the latter subsuming any other additional task requiring energy) between ectothermic and endothermic vertebrates...
February 13, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29449410/conserved-microrna-targeting-reveals-preexisting-gene-dosage-sensitivities-that-shaped-amniote-sex-chromosome-evolution
#4
Sahin Naqvi, Daniel W Bellott, Kathy S Lin, David C Page
Mammalian X and Y Chromosomes evolved from an ordinary autosomal pair. Genetic decay of the Y led to X Chromosome inactivation (XCI) in females, but some Y-linked genes were retained during the course of sex chromosome evolution, and many X-linked genes did not become subject to XCI. We reconstructed gene-by-gene dosage sensitivities on the ancestral autosomes through phylogenetic analysis of microRNA (miRNA) target sites and compared these preexisting characteristics to the current status of Y-linked and X-linked genes in mammals...
February 15, 2018: Genome Research
https://www.readbyqxmd.com/read/29447391/comparative-analysis-of-epidermal-differentiation-genes-of-crocodilians-suggests-new-models-for-the-evolutionary-origin-of-avian-feather-proteins
#5
Karin Brigit Holthaus, Bettina Strasser, Julia Lachner, Supawadee Sukseree, Wolfgang Sipos, Anton Weissenbacher, Erwin Tschachler, Lorenzo Alibardi, Leopold Eckhart
The epidermis of amniotes forms a protective barrier against the environment and the differentiation program of keratinocytes, the main cell type in the epidermis, has undergone specific alterations in the course of adaptation of amniotes to a broad variety of environments and lifestyles. The epidermal differentiation complex (EDC) is a cluster of genes expressed at late stages of keratinocyte differentiation in both sauropsids and mammals. In the present study we identified and analyzed the crocodilian equivalent of the EDC...
February 12, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29446682/fetal-urinary-tract-anomalies-review-of-pathophysiology-imaging-and-management
#6
Achille Mileto, Malak Itani, Douglas S Katz, Joseph R Siebert, Manjiri K Dighe, Theodore J Dubinsky, Mariam Moshiri
OBJECTIVE: Common fetal anomalies of the kidneys and urinary tract encompass a complex spectrum of abnormalities that can be detected prenatally by ultrasound. Common fetal anomalies of the kidneys and urinary tract can affect amniotic fluid volume production with the development of oligohydramnios or anhydramnios, resulting in fetal pulmonary hypoplasia and, potentially, abnormal development of other fetal structures. CONCLUSION: We provide an overview of common fetal anomalies of the kidneys and urinary tract with an emphasis on sonographic patterns as well as pathologic and postnatal correlation, along with brief recommendations for postnatal management...
February 15, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29444982/the-unique-features-of-proteins-depicting-the-chicken-amniotic-fluid
#7
Mylène Da Silva, Clara Dombre, Aurélien Brionne, Philippe Monget, Magali Chessé, Marion De Pauw, Maryse Mills, Lucie Combes-Soia, Valérie Labas, Nicolas Guyot, Yves Nys, Sophie Rehault-Godbert
In many amniotes, the amniotic fluid is depicted as a dynamic milieu that participates in the protection of the embryo (cushioning, hydration, and immunity). However, in birds, the protein profile of the amniotic fluid remains unexplored, even though its proteomic signature is predicted to differ compared to human's. In fact, unlike humans, chicken amniotic fluid does not collect excretory products and its protein composition strikingly changes at mid-development due to the massive inflow of egg white proteins, which are thereafter swallowed by the embryo to support its growth...
February 14, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29443075/human-pluripotent-stem-cell-culture-on-polyvinyl-alcohol-co-itaconic-acid-hydrogels-with-varying-stiffness-under-xeno-free-conditions
#8
Tzu-Cheng Sung, Hsing-Fen Li, Akon Higuchi, Qing-Dong Ling, Jia-Sin Yang, Yeh-Chia Tseng, Chih-Hsien Pan Pan, Abdullah A Alarfaj, Murugan A Munusamy, Suresh Kumar, Shih-Tien Hsu, Kadarkarai Murugan
The effect of physical cues, such as the stiffness of biomaterials on the proliferation and differentiation of stem cells, has been investigated by several researchers. However, most of these investigators have used polyacrylamide hydrogels for stem cell culture in their studies. Therefore, their results are controversial because those results might originate from the specific characteristics of the polyacrylamide and not from the physical cue (stiffness) of the biomaterials. Here, we describe a protocol for preparing hydrogels, which are not based on polyacrylamide, where various stem, cells including human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells, can be cultured...
February 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29440793/use-of-an-amniotic-membrane-as-a-novel-barrier-in-a-tooth-with-a-questionable-prognosis
#9
Kanakamedala Anil Kumar, Muppalla Chakravarthy, Shalini Selvarajan, Theyagarajan Ramakrishnan, Geetha Ari
The chief goal of combination therapy using guided tissue regeneration along with bone grafts is to optimally and predictably regenerate the periodontal attachment apparatus. The evolution of regenerative therapy along with careful patient selection and treatment planning can have positive results even in cases which are not ideal. The present case report describes a tooth with a questionable prognosis treated successively by endodontic then periodontal therapy using an amniotic membrane along with a bone graft to treat an intrabony defect...
May 2017: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/29439621/biofluid-metabolomics-in-preterm-birth-research
#10
Ana M Gil, Daniela Duarte
This article presents an account of the research carried out so far in the use of metabolomics to find biomarkers of preterm birth (PTB) in fetal, maternal, and newborn biofluids. Metabolomic studies have employed mainly nuclear magnetic resonance spectroscopy or mass spectrometry-based methodologies to analyze, on one hand, prenatal biofluids (amniotic fluid, maternal urine/maternal blood, cervicovaginal fluid) to identify predictive biomarkers of PTB, and on the other hand, biofluids collected at or after birth (amniotic fluid, umbilical cord blood, newborn urine, and newborn blood, maternal blood, or breast milk) to assess and follow up the health status of PTB babies...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29436503/diversity-change-during-the-rise-of-tetrapods-and-the-impact-of-the-carboniferous-rainforest-collapse
#11
Emma M Dunne, Roger A Close, David J Button, Neil Brocklehurst, Daniel D Cashmore, Graeme T Lloyd, Richard J Butler
The Carboniferous and early Permian were critical intervals in the diversification of early four-limbed vertebrates (tetrapods), yet the major patterns of diversity and biogeography during this time remain unresolved. Previous estimates suggest that global tetrapod diversity rose continuously across this interval and that habitat fragmentation following the 'Carboniferous rainforest collapse' (CRC) drove increased endemism among communities. However, previous work failed to adequately account for spatial and temporal biases in sampling...
February 14, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29434245/galectin-3-plays-an-important-role-in-preterm-birth-caused-by-dental-infection-of-porphyromonas-gingivalis
#12
Mutsumi Miyauchi, Min Ao, Hisako Furusho, Chanbora Chea, Atsuhiro Nagasaki, Shinnichi Sakamoto, Toshinori Ando, Toshihiro Inubushi, Katsuyuki Kozai, Takashi Takata
Dental infection is risk for preterm birth (PTB) through unclear mechanisms. We established a dental infection-induced PTB mouse model, in which Porphyromonas gingivalis (P.g.) induced PTB by 2 days. We analysed pathogenic factors contributing to PTB and their effects on trophoblasts in vitro. TNF-α, IL-8, and COX-2 were upregulated in P.g.-infected placenta. Galectin-3 (Gal-3), an immune regulator, was significantly upregulated in placenta, amniotic fluid, and serum. In vitro, P.g.-lipopolysaccharide (P.g...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29430874/characterizing-human-decellularized-crystalline-lens-capsules-as-a-scaffold-for-corneal-endothelial-tissue-engineering
#13
Bert Van den Bogerd, Sorcha Ní Dhubhghaill, Nadia Zakaria
The idea of transplanting a sheet of laboratory-grown corneal endothelium dates back to 1978; however, the ideal scaffold is still lacking. We hypothesized that human crystalline lens capsules (LCs) could qualify as a scaffold and aimed to characterize the properties of this material for endothelial tissue engineering. LCs were isolated from donor eyes, stored at -80 °C, and decellularized with water and trypsin-EDTA. The decellularization was investigated by nuclear staining and counting and the capsule thickness was determined by optical coherence tomography and compared with Descemet's membrane (DM)...
December 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29429293/-deep-anterior-lamellar-keratoplasty-combined-with-antiviral-therapy-in-the-treatment-of-severe-herpes-necrotizing-stromal-keratitis
#14
S X Li, J T Wang, Y Jiang, X Wang, W Y Shi
Objective: To evaluate the efficacy of modified deep lamellar keratoplasty (DLKP) combined with antiviral medications for severe herpes necrotizing stromal keratitis. Methods: Retrospective case series study. Modified DLKP was performed in combination with antiviral medications in fifty patients (50 eyes) with severe necrotizing stromal keratitis, which was unresponsive to systemic and topical antiviral treatment for 1 week, at Shandong Eye Hospital. Before surgery, the operated eyes were examined using slit-lamp microscopy...
February 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29428266/duraplasty-using-amniotic-membrane-versus-temporal-muscle-fascia-a-clinical-comparative-study
#15
Agus Turchan, Taufiq Fatchur Rochman, Arie Ibrahim, Dyah Fauziah, Joni Wahyuhadi, M Arifin Parenrengi, Asra Al Fauzi, Erliano Sufarnap, Abdul Hafid Bajamal, Ferdiansyah, Heri Suroto, Purwati, Fedik Abdul Rantam, Adanti Wido Paramadini, Christianto Benjamin Lumenta
In the field of neurosurgery, often the dura mater cannot be sutured, and consequently, it requires a duraplasty procedure using a dural fascial graft. Since 1890, various materials have been researched as dura mater substitutes. Amniotic membrane, for example, is suitable as a dural graft material and has been used in neurosurgery since 2012. However, there has been little research on human patient's dural healing after the use of amniotic membrane in their duraplasty procedure. To address this gap, a clinical experimental study was undertaken to evaluate the human dural healing of 16 patients who had undergone duraplasty in decompressive craniectomy surgery at Dr...
February 7, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29426435/sural-nerve-graft-for-neurotrophic-keratitis-early-results
#16
Ezekiel Weis, Avi Rubinov, Ahmed Rustom Al-Ghoul, Farrah Meng-Kay Yau
OBJECTIVE: To report our center's experience in treating neurotrophic keratitis (NK) through corneal neurotization using nerve transfers and sural nerve grafts in the adult population. DESIGN: Prospective observational case series, single center study. METHODS: Six consecutive patients (5 males and 1 female) with severe NK were evaluated. All patients had complete corneal anaesthesia in the neurotrophic eye. Three patients had unstable epithelial disease with recurrent breakdowns despite maximal medical and standard surgical management; three presented with dense scarring of their cornea...
February 2018: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/29426264/comparison-between-maternal-and-neonatal-outcome-of-pprom-in-the-cases-of-amniotic-fluid-index-afi-of-more-and-less-than-5-cm
#17
Ashraf Sadat Mousavi, Neda Hashemi, Maryam Kashanian, Narges Sheikhansari, Arash Bordbar, Shayesteh Parashi
The study was performed on pregnant women with a gestational age of 26-32 weeks of pregnancy, who had been admitted to the hospital with a confirmed diagnosis of premature rupture of membranes. In all eligible women, ultrasounds were performed for the evaluation of amniotic fluid index. Then, the women were divided into two groups according to amniotic fluid index of ≥5 cm and <5 cm. These women were followed and monitored up to delivery. The women of the two groups did not have significant difference between them according to age, gestational age at the time of ruptured membrane, body mass index, gravidity, parity, gestational age at delivery and route of delivery...
February 9, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29425317/human-cervical-mucus-plugs-exhibit-insufficiencies-in-antimicrobial-activity-towards-group-b-streptococcus
#18
Jay Vornhagen, Phoenicia Quach, Verónica Santana-Ufret, Varchita Alishetti, Alyssa Brokaw, Blair Armistead, Hai Qing Tang, James W MacDonald, Theodor K Bammler, Kristina M Adams Waldorf, Niels Uldbjerg, Lakshmi Rajagopal
Preterm birth is a leading cause of neonatal mortality and lacks an effective therapy. Ascending microbial infections from the lower genital tract lead to infection of the placenta, amniotic fluid and fetus causing preterm birth or stillbirth. Directly in the path of an ascending infection is the cervical mucus plug (CMP), a dense mucoid structure in the cervical canal with potential antimicrobial properties. In this study, we aimed to define the components of CMP responsible for antimicrobial activity against a common lower genital tract organism associated with preterm birth and stillbirths, namely Group B Streptococcus (GBS)...
February 7, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29424945/in-vitro-and-in-vivo-investigation-of-a-novel-amniotic-based-chitosan-dressing-for-wound-healing
#19
Maryam Momeni, Mohammad Zarehaghighi, Mirhamed Hajimiri, Ghasemali Khorasani, Rassoul Dinarvand, Abdolhossein Nekookar, Niloofar Sodeifi, Pardis Khosravani, Niloofar Shayanasl, Marzieh Ebrahimi
It is more than a decade that amniotic membrane has been used as a wound dressing because of its anti-inflammatory, anti-microbial, anti-fibrotic, anti-scaring properties, as well as its pain relieving and epithelialization promoting features. However, amniotic membrane had limited applications because it needs to suture in surgery, is highly fragile, firmly adhere to the wound and may cause bleeding and pain when changing the bandage. This study investigated the possibility of development of a novel amniotic-based chitosan gel dressing as a potential wound repair substrate with marked efficacy...
February 9, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29422028/the-curious-case-of-peroxiredoxin-5-what-its-absence-in-aves-can-tell-us-and-how-it-can-be-used
#20
Marc Pirson, André Clippe, Bernard Knoops
BACKGROUND: Peroxiredoxins are ubiquitous thiol-dependent peroxidases that represent a major antioxidant defense in both prokaryotic cells and eukaryotic organisms. Among the six vertebrate peroxiredoxin isoforms, peroxiredoxin-5 (PRDX5) appears to be a particular peroxiredoxin, displaying a different catalytic mechanism, as well as a wider substrate specificity and subcellular distribution. In addition, several evolutionary peculiarities, such as loss of subcellular targeting in certain species, have been reported for this enzyme...
February 8, 2018: BMC Evolutionary Biology
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