keyword
MENU ▼
Read by QxMD icon Read
search

amniotic

keyword
https://www.readbyqxmd.com/read/29691598/hepatocyte-transplantation-and-advancements-in-alternative-cell-sources-for-liver-based-regenerative-medicine
#1
REVIEW
Charlotte A Lee, Siddharth Sinha, Emer Fitzpatrick, Anil Dhawan
Human hepatocyte transplantation has been actively perused as an alternative to liver replacement for acute liver failure and liver-based metabolic defects. Current challenges in this field include a limited cell source, reduced cell viability following cryopreservation and poor engraftment of cells into the recipient liver with consequent limited life span. As a result, alternative stem cell sources such as pluripotent stem cells, fibroblasts, hepatic progenitor cells, amniotic epithelial cells and mesenchymal stem/stromal cells (MSCs) can be used to generate induced hepatocyte like cells (HLC) with each technique exhibiting advantages and disadvantages...
April 24, 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29691387/fetal-demise-and-failed-antibody-therapy-during-zika-virus-infection-of-pregnant-macaques
#2
Diogo M Magnani, Thomas F Rogers, Nicholas J Maness, Nathan D Grubaugh, Nathan Beutler, Varian K Bailey, Lucas Gonzalez-Nieto, Martin J Gutman, Núria Pedreño-Lopez, Jaclyn M Kwal, Michael J Ricciardi, Tereance A Myers, Justin G Julander, Rudolf P Bohm, Margaret H Gilbert, Faith Schiro, Pyone P Aye, Robert V Blair, Mauricio A Martins, Kathrine P Falkenstein, Amitinder Kaur, Christine L Curry, Esper G Kallas, Ronald C Desrosiers, Pascal J Goldschmidt-Clermont, Stephen S Whitehead, Kristian G Andersen, Myrna C Bonaldo, Andrew A Lackner, Antonito T Panganiban, Dennis R Burton, David I Watkins
Zika virus (ZIKV) infection of pregnant women is associated with pathologic complications of fetal development. Here, we infect pregnant rhesus macaques (Macaca mulatta) with a minimally passaged ZIKV isolate from Rio de Janeiro, where a high rate of fetal development complications was observed. The infection of pregnant macaques with this virus results in maternal viremia, virus crossing into the amniotic fluid (AF), and in utero fetal deaths. We also treated three additional ZIKV-infected pregnant macaques with a cocktail of ZIKV-neutralizing human monoclonal antibodies (nmAbs) at peak viremia...
April 24, 2018: Nature Communications
https://www.readbyqxmd.com/read/29690856/human-amniotic-membrane-with-aligned-electrospun-fiber-as-scaffold-for-aligned-tissue-regeneration
#3
Hanis Nazihah Hasmad, Mohd Reusmaazran Yusof, Zainul Rashid Mohd Razi, Ruszymah Binti Haji Idrus, Shiplu Roy Chowdhury
Fabrication of composite scaffolds is one of the strategies proposed to enhance the functionality of tissue engineered scaffolds for improved tissue regeneration. By combining multiple elements together, unique biomimetic scaffolds with desirable physical and mechanical properties can be tailored for tissue-specific applications. Despite having a highly porous structure, the utility of electrospun fibers (EF) as scaffold is usually hampered by their insufficient mechanical strength. In this study, we attempted to produce a mechanically competent scaffold with cell-guiding ability by fabricating aligned PLGA fibers on decellularized human amniotic membrane (HAM), known to possess favorable tensile and wound healing properties...
April 25, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29690730/-a-clinical-retrospective-study-on-160-cases-of-multiple-umbilical-cord-around-the-neck
#4
Y Q Zhang, W Zhao, K T Chu, Y Zhao, L P Chen, Y Yu, X J Han
Objective: To compare the neonatal and maternal outcomes between the patients with umbilical cord around the neck (≥3 loops) and with (1 or 2 loops). Methods: A retrospective analysis was conducted on the clinical data of 160 cases with multiple umbilical cord around the neck (≥3 loops) in the Department of Obstetrics, Women's Hospital School of Medicine Zhejiang University between January 2014 and April 2017.For each case, two control women who gave birth at the same day with vertex position and singletons were selected...
April 17, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29689632/a-crossover-comparison-of-standard-and-telerobotic-approaches-to-prenatal-sonography
#5
Scott J Adams, Brent E Burbridge, Andreea Badea, Nadine Kanigan, Luis Bustamante, Paul Babyn, Ivar Mendez
OBJECTIVES: To determine the feasibility of a telerobotic approach to remotely perform prenatal sonographic examinations. METHODS: Thirty participants were prospectively recruited. Participants underwent a limited examination (assessing biometry, placental location, and amniotic fluid; n = 20) or a detailed examination (biometry, placental location, amniotic fluid, and fetal anatomic survey; n = 10) performed with a conventional ultrasound system. This examination was followed by an equivalent examination performed with a telerobotic ultrasound system, which enabled sonographers to remotely control all ultrasound settings and fine movements of the ultrasound transducer from a distance...
April 24, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/29689547/sirt3-facilitates-amniotic-fluid-stem-cells-to-repair-diabetic-nephropathy-through-protecting-mitochondrial-homeostasis-by-modulation-of-mitophagy
#6
Jianxun Feng, Chang Lu, Qin Dai, Junqin Sheng, Min Xu
BACKGROUND/AIMS: Amniotic fluid stem cells (AFSCs) transplantation is a promising therapeutic strategy for diabetic nephropathy. Sirtuin3 (SIRT3) is a novel mitochondrial protective factor. In the present study, we aimed to investigate whether SIRT3 protects against hyperglycemia-induced AFSCs damage and enhances the therapeutic efficiency of AFSCs in diabetic nephropathy. METHODS: To establish the diabetic nephropathy model, db/ db mice were used. AFSCs were obtained and transplanted into the kidney tissue of db/ db mice...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29688163/human-amniotic-fluid-stem-cells-hafscs-expressing-p21-and-cyclin-d1-genes-retain-excellent-viability-after-freezing-with-dimethyl-sulfoxide-dmso
#7
Shiva Gholizadeh-Ghaleh Aziz, Zahra Fardyazar, Fatima Pashaei-Asl, Mohammad Rahmati-Yamchi, Khodadad Khodadadi, Maryam Pashaiasl
Human amniotic fluid stem cells (hAFSCs) have features intermediate between embryonic and adult SCs, can differentiate into lineages of all three germ layers, and do not develop into tumors in vivo. Moreover, hAFSCs can be easily obtained in routine procedures and there is no ethical or legal limitations regarding their use for clinical and experimental applications. The aim of this study was to assess the effect of slow freezing/thawing and two different concentrations of DMSO (10% DMSO + 90% fetal bovine serum [FBS] and 5% DMSO + 95% FBS) on the survival of hAFSCs...
April 24, 2018: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29687742/amniotic-membrane-and-its-epithelial-and-mesenchymal-stem-cells-as-an-appropriate-source-for-skin-tissue-engineering-and-regenerative-medicine
#8
Behrouz Farhadihosseinabadi, Mehrdad Farahani, Tahereh Tayebi, Ameneh Jafari, Felor Biniazan, Khashayar Modaresifar, Hamideh Moravvej, Soheyl Bahrami, Heinz Redl, Lobat Tayebi, Hassan Niknejad
One of the main goals of tissue engineering and regenerative medicine is to develop skin substitutes for treating deep dermal and full thickness wounds. In this regard, both scaffold and cell source have a fundamental role to achieve exactly the same histological and physiological analog of skin. Amnion epithelial and mesenchymal cells possess the characteristics of pluripotent stem cells which have the capability to differentiate into all three germ layers and can be obtained without any ethical concern. Amniotic cells also produce different growth factors, angio-modulatory cytokines, anti-bacterial peptides and a wide range of anti-inflammatory agents which eventually cause acceleration in wound healing...
April 24, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29686390/tissue-remodeling-after-ocular-surface-reconstruction-with-denuded-amniotic-membrane
#9
Jing Jie, Jie Yang, Hui He, Jianlan Zheng, Wenyan Wang, Liying Zhang, Zhiyuan Li, Jingyao Chen, M Vimalin Jeyalatha, Nuo Dong, Huping Wu, Zuguo Liu, Wei Li
Amniotic membrane (AM) has been widely used as a temporary or permanent graft in the treatment of various ocular surface diseases. In this study, we compared the epithelial wound healing and tissue remodeling after ocular surface reconstruction with intact amniotic membrane (iAM) or denuded amniotic membrane (dAM). Partial limbal and bulbar conjunctival removal was performed on New Zealand rabbits followed by transplantation of cryo-preserved human iAM or dAM. In vivo observation showed that the epithelial ingrowth was faster on dAM compared to iAM after AM transplantation...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29686267/amniotic-fluid-c-reactive-protein-as-a-predictor-of-infection-in-caesarean-section-a-feasibility-study
#10
Zbigniew Marchocki, Angela Vinturache, Kevin Collins, Paddy O' Reilly, Keelin O'Donoghue
This study evaluated the feasibility of maternal C-reactive protein (CRP) in amniotic fluid (AF) as a predictor of post-partum infection in women who undergo emergency or elective caesarean section (CS). AF bacterial culture and levels of hs-CRP in maternal serum and AF were evaluated in Day 0 and three days thereafter (Day 3) in 79 women undergoing CS. Univariate analyses assessed the clinical and demographic characteristics, whereas the ROC curves assessed the feasibility of hs-CRP as marker of inflammation in women who undergo CS...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29685081/longitudinal-trajectory-of-vulvovaginal-candidiasis-trichomoniasis-and-bacterial-vaginosis-during-pregnancy-as-well-as-the-impact-on-pregnancy-outcome-a-preliminary-study
#11
Cheng-Yang Hu, Feng-Li Li, Xiao-Guo Hua, Wen-Jiang, Xiu-Jun Zhang
OBJECTIVES: The aims of this study were to describe the course of vulvovaginal candidiasis (VVC), trichomoniasis, and bacterial vaginosis (BV) in pregnancy and to explore the association between these longitudinal changes and pregnancy outcomes. METHODS: A total of 793 pregnant women were enrolled at the first trimester of pregnancy and were followed prospectively twice at the second and third trimester. VVC, trichomoniasis, and BV were evaluated at each trimester of pregnancy...
April 23, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29685073/the-association-of-second-trimester-biomarkers-in-amniotic-fluid-and-fetal-outcome
#12
Irene Sterpu, Peter Anfelter, Susan Wray, Helena Kaihola, Helena Åkerud, Eva Wiberg-Itzel
OBJECTIVE: To identify the level of amniotic fluid lactate (AFL), placental growth factor (PLGF) and vascular endothelial growth factor (VEGF) at second trimester amniocentesis, and to compare levels in normal pregnancies with pregnancies ending in a miscarriage, an intrauterine growth restricted fetus (IUGR) or decreased fetal movements. STUDY DESIGN: A prospective cohort study. Amniotic fluid was consecutive collected at amniocentesis in 106 pregnancies. Fetal wellbeing at delivery was evaluated from medical files and compared with the levels of AFL, VEGF and PLGF at the time of amniocentesis...
April 23, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29684686/a-comprehensive-profile-and-inter-individual-variations-analysis-of-the-human-normal-amniotic-fluid-proteome
#13
Xiang Liu, Yijun Song, Zhengguang Guo, Wei Sun, Juntao Liu
Amniotic fluid contains large amounts of proteins produced by amnion epithelial cells, fetal tissues, fetal excretions and placental tissues; thus, it is an important potential source of biomarkers for identifying fetal pathologies. In this study, a pooled AF sample from 7 healthy volunteers was used to provide a comprehensive profile of normal human AF proteome using immunoaffinity depletion of 14 high-abundance proteins. Each individual AF sample was used to analyze inter-individual variations with iTRAQ method...
April 20, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29682688/what-is-the-benefit-of-using-amniotic-membrane-in-oral-surgery-a-comprehensive-review-of-clinical-studies
#14
REVIEW
M Fénelon, S Catros, J C Fricain
OBJECTIVES: Since its first use for the reconstruction of tissue defects in the oral cavity in 1985, human amniotic membrane (hAM) has been widely studied in the field of oral surgery. Despite the growing number of publications in this field, there is no systematic review or meta-analysis concerning its clinical applications, outcome assessments, and relevance in oral surgery. The aim of this review is to provide a thorough understanding of the potential use of hAM for soft and hard tissue reconstruction in the oral cavity...
April 22, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29681834/amnwiotic-membrane-transplantation-as-a-treatment-for-sterile-infiltration-and-corneal-melting-after-corneal-crosslinking-for-keratoconus
#15
Takanori Sasaki, Takeshi Ide, Ikuko Toda, Naoko Kato
We report a case of corneal melting through sterile infiltration presumably due to excessive use of nonsteroidal anti-inflammatory eye drops after corneal crosslinking (CXL). It was treated using steroids combined with amniotic membrane transplantation (AMT). A 33-year-old man with progressing keratoconus underwent left eye CXL. We prescribed betamethasone and levofloxacin eye drops 5 times daily and diclofenac sodium eye drops 3 times daily for 3 days. Three days after CXL, there was a persistent epithelial defect and the left corneal stroma was clouded...
January 2018: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/29678852/whole-blood-pcr-for-rapid-screening-of-%C3%AE-0-thalassemia
#16
Phongsathorn Wichian, Supawadee Yamsri, Kanokwan Sanchaisuriya, Goonnapa Fucharoen, Supan Fucharoen
Hemoglobin Bart's hydrops fetalis (homozygous α0 -thalassemia) is the most severe form of thalassemia in the Southeast Asian population. Fetuses with this disorder almost always die in utero or shortly after birth. Screening of α0 -thalassemia carrier is therefore crucial. Currently, diagnosis of α0 -thalassemia genes is done by DNA-based analysis which relies on DNA extraction. We have developed a simple screening format based on whole blood PCR assay. The method was validated on 198 specimens and the results show 100% concordance with a conventional gap-PCR on DNA specimens...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29678840/micronuclei-induction-in-amniotic-fluid-cells-from-cyclophosphamide-treated-rats
#17
Karen Gomez-Mariscal, Belinda C Gómez-Meda, Ana L Zamora-Perez, María G Sánchez-Parada, Martha P Gallegos-Arreola, Guillermo M Zúñiga-González
The aim of the study was to determine if amniotic fluid cells of rats can be used to provide evidence of genotoxicity. In order to do that micronuclei formation was induced in rats during pregnancy after treatment with cyclophosphamide (CP), at different CP doses. On gestational day 19, we collected the amniotic fluid and determined the frequency of micronucleated cells (MNCs) from the offspring. Samples were centrifuged and placed on clean slides. The smears were observed with an epifluorescence microscope...
March 2018: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29678720/a-completely-human-derived-biomaterial-mimicking-limbal-niche-platelet-rich-fibrin-gel
#18
Gamze Dereli Can, Özge Ekin Akdere, Mehmet Erol Can, Bahri Aydın, Nurullah Cagil, Menemşe Gümüşderelioğlu
Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vitro cell culture. Here, custom-modified human-derived PRF (HPRF) was produced via different centrifugation protocols, then, characterized by morphologically and chemically and utilized as a substrate and as a conditioned medium for limbal explant culture for the first time...
April 17, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29678510/corrigendum-to-dual-chemotactic-factors-secreting-human-amniotic-mesenchymal-stem-cells-via-talen-mediated-gene-editing-enhanced-angiogenesis-int-j-cardiol-260-2018-156-162
#19
In Sil Jeong, Youngjin Park, Hyun Aae Ryu, Hyun Sook An, Ju Hye Han, Sung-Whan Kim
No abstract text is available yet for this article.
April 18, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29677005/maged2-a-novel-form-of-antenatal-bartter-s-syndrome
#20
Martin Kömhoff, Kamel Laghmani
PURPOSE OF REVIEW: Antenatal Bartter's syndrome (aBS) is the most severe form of Bartter's syndrome, requiring close follow-up, in particular during the neonatal period, primarily because of prematurity. The recent identification of a novel and very severe form of aBS merits an update on this topic. RECENT FINDING: Despite the identification of several genes involved in Bartter's syndrome, about 20% of patients clinically diagnosed with aBS remained without genetic explanation for decades...
April 18, 2018: Current Opinion in Nephrology and Hypertension
keyword
keyword
33138
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"