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https://www.readbyqxmd.com/read/28820372/microcephaly-caused-by-lymphocytic-choriomeningitis-virus
#1
Maia Delaine, Anne-Sophie Weingertner, Antoine Nougairede, Quentin Lepiller, Samira Fafi-Kremer, Romain Favre, Rémi Charrel
We report congenital microencephaly caused by infection with lymphocytic choriomeningitis virus in the fetus of a 29-year-old pregnant women at 23 weeks' gestation. The diagnosis was made by ultrasonography and negative results for other agents and confirmed by a positive PCR result for lymphocytic choriomeningitis virus in an amniotic fluid sample.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28819943/the-mmp-9-timp-1-imbalance-and-the-reduced-level-of-tgf-%C3%AE-in-the-cervical-area-of-amniotic-membrane-is-a-possible-risk-factor-of-prom-and-premature-labor-proof-of-concept-study
#2
Małgorzata Litwiniuk, Małgorzata Radowicka, Alicja Krejner, Mirosław Wielgoś, Tomasz Grzela
OBJECTIVES: To assess the level MMP-9, TIMP-1 and TGF-β in placental and cervical region of amniotic membranes derived from at-term, pre-term and PROM deliveries. MATERIAL AND METHODS: 14 amniotic membranes have been assessed; the quantitative analysis of MMP-9, TGF-β and TIMP-1 was assayed using respective Quantikine Immunoassay Kit. RESULTS: The MMP-9 level in PROM samples was similar to the level of MMP-9 in at-term membranes and comparable between the cervical and placental region of these membranes...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28817205/amniotic-fluid-embolism-mortality-rate
#3
Michael D Benson
AIM: The objective of this study was to determine the mortality rate of amniotic fluid embolism (AFE) using population-based studies and case series. METHODS: A literature search was conducted using the two key words: 'amniotic fluid embolism (AFE)' AND 'mortality rate'. Thirteen population-based studies were evaluated, as well as 36 case series including at least two patients. RESULTS: The mortality rate from population-based studies varied from 11% to 44%...
August 17, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#4
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
August 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28813455/maternal-serum-c-reactive-protein-concentration-and-intra-amniotic-inflammation-in-women-with-preterm-prelabor-rupture-of-membranes
#5
Ivana Musilova, Marian Kacerovsky, Martin Stepan, Tomas Bestvina, Lenka Pliskova, Barbora Zednikova, Bo Jacobsson
OBJECTIVE: To evaluate maternal serum C-reactive protein (CRP) concentrations in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) in relation to the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). METHODS: Two hundred and eighty-seven women with singleton pregnancies complicated by PPROM between 2014 and 2016 were included in this study. Maternal blood and amniotic fluid samples were collected at the time of admission...
2017: PloS One
https://www.readbyqxmd.com/read/28812724/the-genomic-landscape-of-evolutionary-convergence-in-mammals-birds-and-reptiles
#6
Liron Levin, Dan Mishmar
Many lineage-defining (nodal) mutations possess high functionality. However, differentiating adaptive nodal mutations from those that are functionally compensated remains challenging. To address this challenge, we identified functional nodal mutations (fNMs) in ~3,400 nuclear DNA (nDNA) and 4 mitochondrial DNA (mtDNA) protein structures from 91 and 1,003 species, respectively, representing the entire mammalian, bird and reptile phylogeny. A screen for candidate compensatory mutations among co-occurring amino acid changes in close structural proximity revealed that such compensated fNMs encompass 37% and 27% of the mtDNA and nDNA datasets, respectively...
February 13, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28809033/somitogenesis-and-axial-development-in-reptiles
#7
Cindy Xu, Mariana B Grizante, Kenro Kusumi
Among amniote vertebrates, reptiles display the greatest variation in axial skeleton morphology. Only recently have they been used in gene expression studies of somitogenesis , challenging previous assumptions about the segmentation clock and axial patterning. An increasing number of reptile genomes and transcriptomes are becoming available as next-generation sequencing becomes more affordable. Information regarding gene sequence and structure can be used to design and synthesize labeled riboprobes by in vitro transcription for gene expression analysis by in situ hybridization, thus, enabling the characterization of spatial and temporal expression patterns of genes involved in somitogenesis, a topic of great interest within evolutionary developmental studies of vertebrates...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809032/a-sensitive-and-versatile-in-situ-hybridization-protocol-for-gene-expression-analysis-in-developing-amniote-brains
#8
Pei-Shan Hou, Takuma Kumamoto, Carina Hanashima
The detection of specific RNA molecules in embryonic tissues has wide research applications including studying gene expression dynamics in brain development and evolution. Recent advances in sequencing technologies have introduced new animal models to explore the molecular principles underlying the assembly and diversification of brain circuits between different amniote species. Here, we provide a step-by-step protocol for a versatile in situ hybridization method that is immediately applicable to a range of amniote embryos including zebra finch and Madagascar ground gecko, two new model organisms that have rapidly emerged for comparative brain studies over recent years...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809029/model-clades-versus-model-species-anolis-lizards-as-an-integrative-model-of-anatomical-evolution
#9
Thomas J Sanger, Bonnie K Kircher
Anolis lizards , known for their replicated patterns of morphological diversification, are widely studied in the fields of evolution and ecology. As a textbook example of adaptive radiation, this genus has supported decades of intense study in natural history, behavior, morphological evolution, and systematics. Following the publication of the A. carolinensis genome, research on Anolis lizards has expanded into new areas, toward obtaining an understanding the developmental and genetic bases of anole diversity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809028/lifting-the-veil-on-reptile-embryology-the-veiled-chameleon-chamaeleo-calyptratus-as-a-model-system-to-study-reptilian-development
#10
Raul E Diaz, Federica Bertocchini, Paul A Trainor
Living amniotes comprise three major phylogenetic lineages: mammals, birds, and non-avian reptiles. Mouse and avian embryos continue to be the primary species used in experimental settings to further our knowledge and understanding of the genetics and embryology of amniotes. In comparison, non-avian reptiles, which constitute up to 40% of all living amniotes, have played a comparatively minor role. Studies of non-avian reptiles are, however, paramount for providing insights into the evolutionary changes that occurred in the transition from reptilian-like amniote ancestors to derived mammalian and avian species...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28809027/in-vitro-and-ex-ovo-culture-of-reptilian-and-avian-neural-progenitor-cells
#11
Wataru Yamashita, Toyo Shimizu, Tadashi Nomura
Reptiles and birds have been highlighted as excellent experimental models for the study of developmental biology; however, due to technical limitations in cellular analysis, dynamics of neural stem/progenitor cells of these animals remain unclear. In this chapter, we introduce the protocols for neurosphere culture and ex ovo embryonic culture of developing reptilian and avian embryos, which are modified from the method originally established for rodent embryos. Applications of these techniques provide powerful strategies for the study of comparative neural development of amniotes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28807901/differing-contributions-of-the-first-and-second-pharyngeal-arches-to-tympanic-membrane-formation-in-the-mouse-and-chick
#12
Toshiko Furutera, Masaki Takechi, Taro Kitazawa, Junko Takei, Takahiko Yamada, Tri Vu Hoang, Filippo M Rijli, Hiroki Kurihara, Shigeru Kuratani, Sachiko Iseki
We have proposed that independent origins of the tympanic membrane (TM), consisting of the external auditory meatus (EAM) and first pharyngeal pouch, are linked with distinctive middle ear structures in terms of dorsal-ventral patterning of the pharyngeal arches during amniote evolution. However, previous studies have suggested that the first pharyngeal arch (PA1) is crucial for TM formation in both the mouse and chick. In this study, we compare TM formation along the anterior-posterior axis in these animals using Hoxa2 expression as a marker of the second pharyngeal arch (PA2)...
August 14, 2017: Development
https://www.readbyqxmd.com/read/28807394/preterm-prelabor-rupture-of-the-membranes-a-disease-of-the-fetal-membranes
#13
Ramkumar Menon, Lauren S Richardson
Preterm prelabor rupture of the membranes (pPROM) remains a significant obstetric problem that affects 3-4% of all pregnancies and precedes 40-50% of all preterm births. pPROM arises from complex, multifaceted pathways. In this review, we summarize some old concepts and introduce some novel theories related to pPROM pathophysiology. Specifically, we introduce the concept that pPROM is a disease of the fetal membranes where inflammation-oxidative stress axis plays a major role in producing pathways that can lead to membrane weakening through a variety of processes...
August 11, 2017: Seminars in Perinatology
https://www.readbyqxmd.com/read/28806949/case-report-of-ocular-kaposi-s-sarcoma
#14
Jing Yang, Xiao-Fang Yin, Yong-Ping Li, Shi-You Zhou
BACKGROUND: Kaposi's sarcoma (KS) is generally considered a neoplastic disorder of vascular origin and occurs in patients with acquired immunodeficiency syndrome (AIDS) or who have received immunosuppressive treatments after an organ transplant (Soulier et al., Blood 86(4):1276-80, 1995; Viejo-Borbolla and Schulz, AIDS Rev 5(4):222-9, 2003; Schulz, J Antimicrob Chemother 45(Suppl T3):15-27, 2000; Aversa et al. Crit Rev Oncol Hematol 53(3):253-65, 2005; Mbulaiteye and Engels, Int J Cancer 119(11):2685-91, 2006; Tessari et al...
August 14, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28806537/effect-of-oral-hydration-on-external-cephalic-version-at-term
#15
Virna Franca Zobbi, Antonella Nespoli, Elisa Spreafico, Roberta Recalcati, Federica Loi, Antonietta Scian, Stefania Galimberti
OBJECTIVE: To evaluate the effect of oral hydration on the success rate of external cephalic version (ECV). DESIGN: Randomized controlled and single-blind trial. SETTING: Academic tertiary hospital with approximately 3,000 births annually. PARTICIPANTS: One hundred sixty-four women at a gestational age of at least 37 weeks with breech-presenting fetuses and normal amniotic fluid indexes (AFIs). METHODS: Participants were randomly assigned to drink 2000 ml or no more than 100 ml of water in the 2 hours before undergoing ECV...
August 11, 2017: Journal of Obstetric, Gynecologic, and Neonatal Nursing: JOGNN
https://www.readbyqxmd.com/read/28805615/prenatal-diagnosis-of-holoprosencephaly-associated-with-smith-lemli-opitz-syndrome-slos-in-a-46-xx-fetus
#16
André Travessa, Patrícia Dias, Pedro Rocha, Ana Berta Sousa
OBJECTIVE: To show the importance of measuring cholesterol precursor levels in amniotic fluid in all pregnancies with ultrasound features (such as holoprosencephaly) suggestive of Smith-Lemli-Opitz syndrome (SLOS), after exclusion of chromosomal anomalies. CASE REPORT: A 28-year-old woman, gravida 1 para 0, performed chorionic villus sampling for fetal karyotyping at 13 weeks of gestation due to positive combined first trimester screening in a fetus with increased nuchal translucency and suspected holoprosencephaly...
August 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28805106/measuring-fetal-brain-and-lung-transcripts-in-amniotic-fluid-supernatant-a-comparison-of-digital-pcr-and-rt-qpcr-methods
#17
Lisa Hui, Sally Beard, Natalie J Hannan
PURPOSE: Amniotic fluid (AF) cell-free RNA is a promising source of information regarding fetal physiology. Digital PCR (dPCR) is a direct approach to nucleic acid detection that reports absolute transcript copy number. The aim of this study was to compare quantification of cell-free fetal brain and lung RNA transcripts in AF by reverse transcription-qPCR and dPCR. MATERIAL AND METHODS: Prospective hospital-based study performed in 2016-2017. Pulmonary genes were quantified in term AF samples collected at elective cesarean birth; neurodevelopmental genes were measured in preterm samples (< 34 weeks) obtained from women undergoing clinically-indicated amniocentesis...
August 14, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28804622/human-placental-growth-hormone-in-normal-and-abnormal-fetal-growth
#18
Alexandros Velegrakis, Maria Sfakiotaki, Stavros Sifakis
Human placental growth hormone (PGH), encoded by the growth hormone (GH) variant gene on chromosome 17, is expressed in the syncytiotrophoblast and extravillous cytotrophoblast layers of the human placenta. Its maternal serum levels increase throughout pregnancy, and gradually replaces the pulsatile secreted pituitary GH. PGH is also detectable in cord blood and in the amniotic fluid. This placental-origin hormone stimulates glyconeogenesis, lipolysis and anabolism in maternal organs, and influences fetal growth, placental development and maternal adaptation to pregnancy...
August 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28804563/count-based-size-correction-analysis-of-maternal-plasma-dna-for-improved-noninvasive-prenatal-detection-of-fetal-trisomies-13-18-and-21
#19
Li Zhang, Qian Zhu, He Wang, Shanling Liu
PURPOSE: Our goal was to derive more sensitive and accurate Z-scores based on combined DNA count- and size-based algorithms to advance molecular diagnostics for noninvasive prenatal testing of fetal trisomies. METHODS: We included 180 cases at high risk for fetal aneuploidy who underwent amniotic fluid cytogenetic analysis. We calculated their traditional count-based Z-scores, as well as their 100-, 130- and 150-, and 166-bp size-corrected Z-scores, and determined each Z-score's reliability based on its comparison to the cases' cytogenetic results...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28803663/simple-method-of-covering-maxillectomy-defects-with-lyophilised-amniotic-membrane
#20
K Hazarika, A K Adhyapok, S C Debnath, K Malik
No abstract text is available yet for this article.
August 10, 2017: British Journal of Oral & Maxillofacial Surgery
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