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https://www.readbyqxmd.com/read/28535541/contribution-of-amniotic-fluid-along-gestation-to-the-prediction-of-perinatal-mortality-in-women-with-early-preterm-premature-rupture-of-membranes
#1
Teresa Cobo, Jordina Munrós, José Ríos, Janisse Ferreri, Federico Migliorelli, Núria Baños, Eduard Gratacós, Montse Palacio
INTRODUCTION: To evaluate the largest vertical pocket (LVP) of amniotic fluid as a time-dependent factor to predict perinatal mortality in women with early preterm premature rupture of membranes (EPPROM). MATERIAL AND METHODS: Observational cohort study of singleton pregnancies with EPPROM <24 weeks. Termination of pregnancy (TOP) was considered if the LVP was <2 cm at 7 days. The maternal and neonatal characteristics of ongoing pregnancies were recorded. Prediction of perinatal mortality was estimated based on the influence of the LVP as a time-dependent factor after adjustment for maternal age, prior invasive procedure, and gestational age at EPPROM...
May 24, 2017: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/28532087/the-repairing-of-full-thickness-skin-deficiency-and-its-biological-mechanism-using-decellularized-human-amniotic-membrane-as-the-wound-dressing
#2
Mengsheng Song, Weiqing Wang, Qihua Ye, Shizhong Bu, Zhisen Shen, Yabin Zhu
Human amniotic membrane (HAM) was a biocompatible scaffold with advantages of anti-inflammatory, low antigen, feasibility, tolerance and low cost. In our previous work, HAM was treated to be decellularized using surfactant, lipase and DNAase methods and the efficacy as an implantable biological mesh was verified after decellularization treatment. In this work, we used the previous protocol to decellularize the fresh HAM, and applied it to repair full-thickness skin defects with Sprague-Dawley rats as the test animals...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529415/using-amniotic-membrane-as-a-novel-method-to-reduce-post-burn-hypertrophic-scar-formation-a-prospective-follow-up-study
#3
Ali Akbar Mohammadi, Shima Eskandari, Hamed Ghoddusi Johari, Ata'ollah Rajabnejad
BACKGROUND: Several studies have shown that the application of amniotic membrane as a biological dressing in the management of burns is accompanied by rapid re-epithelialisation. In this follow-up study, we aimed to evaluate the possible role of amniotic membrane as an adjunct to split thickness skin grafting on reducing itching and severity of hypertrophic scar formation. MATERIALS AND METHODS: From October 2013 to January 2015, in a prospective follow-up study, 54 patients (108 limbs) with second and third degree burns, covering 4%-15% of total body surface area (TBSA), were included in the study...
January 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28524771/mercury-levels-in-parturient-and-newborns-from-aveiro-region-portugal
#4
Ana Catarina Alves, Marta S Monteiro, Ana Luísa Machado, Mário Oliveira, Ana Bóia, Ana Correia, Nuno Oliveira, Amadeu M V M Soares, Susana Loureiro
Since the outbreak of methylmercury (MeHg) poisoning in Japan and Iraq, mercury (Hg) is classified as well-established teratogen. The Portuguese region of Aveiro faced some decades ago an environmental Hg contamination due to activities from a chlor-alkali plant. Until now, no apparent evaluation was conducted regarding prenatal exposure to Hg in this area. The main objectives of this study were to: i) assess maternal and fetal exposure to Hg in the Aveiro region using noninvasive biological matrices; ii) examine the influence of variables that may contribute to Hg exposure during pregnancy; and iii) improve knowledge regarding metal accumulation and distribution over the maternal-fetal-placental unit...
May 19, 2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28523762/a-retrospective-study-of-cytogenetic-results-from-amniotic-fluid-in-5328-fetuses-with-abnormal-obstetric-sonographic-findings
#5
Shuo Zhang, Caixia Lei, Junping Wu, Haiyan Sun, Yuezhou Yang, Yueping Zhang, Xiaoxi Sun
OBJECTIVES: The purpose of this study was to evaluate the diagnostic utility of karyotype analysis of amniotic fluid for fetuses with abnormal sonographic findings and to determine the detection rates of abnormal karyotypes. METHODS: We conducted a retrospective study of 5328 fetuses with abnormal sonographic findings in the first or second trimester enrolled from October 1998 and September 2015. Cytogenetic results from amniotic fluid were obtained in all of these pregnancies...
May 19, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28522073/cell-based-skin-substitutes-accelerate-regeneration-of-extensive-burn-wounds-in-rats
#6
Sadrollah Motamed, Ehsan Taghiabadi, Hojjat Molaei, Niloofar Sodeifi, Seyed Esmaeil Hassanpour, Saeed Shafieyan, Enzollah Azargashb, Fatemeh Farajzadeh-Vajari, Nasser Aghdami, Amir Bajouri
BACKGROUND: This study investigated the effects of amniotic membrane combined with adipose-derived stem cells or fetal fibroblasts on regenerating extensive burns in rats. METHODS: Third degree burns of 1100-1800 mm(2) were induced on 32 Sprague-Dawley rats. Burned sites were excised and randomly covered with Vaseline gauze (control), human amniotic membrane (HAM), human fetal fibroblasts seeded on HAM (HAM-FF), or human adipose-derived stem cells seeded on HAM (HAM-ASC), and followed by wound closure and histological assessments...
April 27, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/28520772/the-chicken-embryo-as-an-efficient-model-to-test-the-function-of-muscle-fusion-genes-in-amniotes
#7
Daniel Sieiro, Nadège Véron, Christophe Marcelle
The fusion of myoblasts into multinucleated myotubes is a crucial step of muscle growth during development and of muscle repair in the adult. While multiple genes were shown to play a role in this process, a vertebrate model where novel candidates can be tested and analyzed at high throughput and relative ease has been lacking. Here, we show that the early chicken embryo is a fast and robust model in which functional testing of muscle fusion candidate genes can be performed. We have used known modulators of muscle fusion, Rac1 and Cdc42, along with the in vivo electroporation of integrated, inducible vectors, to show that the chicken embryo is a suitable model in which their function can be tested and quantified...
2017: PloS One
https://www.readbyqxmd.com/read/28520465/mesenchymal-stem-stromal-cells-in-regenerative-medicine-past-present-and-future
#8
Ourania Trohatou, Maria G Roubelakis
The concept of Regenerative Medicine combined with Cell based Therapy and Tissue Engineering represents the fourth pillar of healthcare and provides a promising approach for the treatment of serious diseases. Recently, cell based therapies are focused on the use of mesenchymal stem/stromal cells (MSCs). Human MSCs, that represent a mesoderm derived population of progenitors, are easily expanded in culture. They are capable to differentiate into osteoblasts, chondrocytes, and adipocytes and exhibit the potential to repair or regenerate damaged tissues...
May 18, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28517044/intercentrum-versus-pleurocentrum-growth-in-early-tetrapods-a-paleohistological-approach
#9
Marylène Danto, Florian Witzmann, Stephanie E Pierce, Nadia B Fröbisch
A variety of vertebral centrum morphologies have evolved within early tetrapods which range from multipartite centra consisting of intercentra and pleurocentra in stem-tetrapods, temnospondyls, seymouriamorphs, and anthracosaurs up to monospondylous centra in lepospondyls. With the present study, we aim to determine the formation of both intercentrum and pleurocentrum and asked whether these can be homologized based on their bone histology. Both intercentra and pleurocentra ossified endochondrally and periosteal bone was subsequently deposited on the outer surface of the centra...
May 18, 2017: Journal of Morphology
https://www.readbyqxmd.com/read/28512421/when-an-easy-thing-goes-wrong-foreign-body-induced-granuloma-associated-scleritis-following-pterygium-surgery
#10
Pedro Coelho, Carlos Menezes, Pedro Rodrigues, Rita Gonçalves, Tiago Maio, Jorge Moreira, Paula Tenedório
PURPOSE: To report a case of pterygium surgery with conjunctival autograft followed by focal necrotizing scleritis due to foreign body entrapment in the scleral bed. CASE REPORT/RESULTS: This is a case report of a 76-years-old male patient who underwent nasal pterygium surgery and developed focal necrotizing scleritis secondary to foreign body entrapment under conjunctival autograft. One month following surgery, slit-lamp examination demonstrated a progressive thinning of the surgical area with focal inflammatory signs...
January 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28511416/correlation-of-cotinine-levels-with-use-of-smokeless-tobacco-mishri-among-pregnant-women-and-anthropometry-of-newborn
#11
Praveen Ganganahalli, Asha Pratinidhi, Jyotsna Patil, Satish V Kakade
INTRODUCTION: 'Smokeless tobacco' is the term used for the tobacco that is consumed in un-burnt form and it can be used orally or nasally. Cotinine, a nicotine metabolite, is used to quantify exposure to tobacco, which readily gains access to foetal circulation. Cotinine is invariably found in coelomic, amniotic and foetal serum when maternal serum cotinine levels exceed 25ng/ml. AIM: To estimate cotinine levels among pregnant women using and not using smokeless tobacco (mishri) and to correlate cotinine level with anthropometry of newborns...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28510071/surgical-techniques-for-open-fetal-repair-of-myelomeningocele
#12
Nalin Gupta
The current standard for fetal repair of myelomeningocele is an open exposure of the gravid uterus with control of the amniotic membranes, and then a multi-layer closure of the fetal defect. The nature of the surgical repair has evolved and requires a series of steps that take into account the delicate nature of the fetal tissues. The individual surgical steps are described including potential pitfalls.
May 16, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28509320/premature-fetal-tissues-are-possible-source-of-valuable-mesenchymal-stem-cells
#13
Filip Andrzej Dąbrowski, Anna Burdzinska, Agnieszka Kulesza, Beata Kaleta, Leszek Pączek, Mirosław Wielgoś
OBJECTIVES: Comparison of the ability to inhibit alloactivated lymphocytes proliferation of human Wharton Jelly (WJ) and amniotic membrane (AM) mesenchymal stem cells (MSCs) from preterm and term pregnancies. MATERIAL AND METHODS: Term-WJ-MSCs (n = 5) and Preterm-WJ-MSCs (n = 1) were obtained from tissue explants by adherent method. Term-AM-MSCs (n = 5) and Preterm-AM-MSCs (n = 1) were obtained by tripsin and collagenase digestion method. Term and Preterm MSCs phenotype was confirmed in vitro by flow cytometry...
2017: Ginekologia Polska
https://www.readbyqxmd.com/read/28508565/menaquinone-4-enhances-osteogenic-potential-of-human-amniotic-fluid-mesenchymal-stem-cells-cultured-in-a-2d-and-3d-dynamic-culture-system
#14
Domitilla Mandatori, Letizia Penolazzi, Caterina Pipino, Pamela Di Tomo, Sara Di Silvestre, Natalia Di Pietro, Sara Trevisani, Marco Angelozzi, Mariangela Ucci, Roberta Piva, Assunta Pandolfi
Menaquinones, also known as Vitamin K2 family, regulate calcium homeostasis in a "bone-vascular cross talk" and recently received particular attention for their positive effect on bone formation. Since the correlation between menaquinones and bone metabolism to date is still unclear, the objective of our study was to investigate the possible role of menaquinone-4 (MK-4), an isoform of the menaquinones family, in the modulation of osteogenesis. For this reason we used a model of human Amniotic Fluid Mesenchymal Stem Cells (hAFMSCs) cultured both in two-dimensional (2D) and three-dimensional (3D, RCCS™bioreactor) in vitro culture systems...
May 16, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28507863/amnion-membrane-in-diabetic-foot-wounds-a-meta-analysis
#15
Alexandra M Haugh, Jacqueline G Witt, Adam Hauch, Michael Darden, Geoffrey Parker, Warren A Ellsworth, Joseph F Buell
BACKGROUND: Amniotic membrane is tissue obtained from human placenta rich in cytokines, growth factors, and stem cells that possess the ability to inhibit infection, improve healing, and stimulate regeneration. METHODS: A meta-analysis was performed examining randomized controlled trials comparing amniotic tissue products with standard of care in nonhealing diabetic foot ulcers including PubMed, Cochrane Central Register of Controlled Trials, and the Cochrane Database of Systematic Reviews...
April 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28506176/neurolysis-with-amniotic-membrane-nerve-wrapping-for-treatment-of-secondary-wartenberg-syndrome-a-preliminary-report
#16
Michael P Gaspar, Patrick M Kane, Michael M Vosbikian, Constantinos Ketonis, Mark S Rekant
BACKGROUND: Entrapment of the superficial sensory branch of the radial nerve (SRN) commonly results in debilitating pain of the dorsoradial wrist. Symptom relief following SRN neurolysis is often incomplete or temporary due to recurrent perineural scarring. METHODS: We performed a retrospective review with prospective follow-up of all patients with SRN neuropathy who were treated with neurolysis and nerve wrapping using an amnion-based allograft adhesion barrier over a one-year interval...
June 2017: Journal of Hand Surgery Asian-Pacific Volume
https://www.readbyqxmd.com/read/28503900/effects-of-in-ovo-feeding-of-l-arginine-on-the-development-of-digestive-organs-intestinal-function-and-post-hatch-performance-of-broiler-embryos-and-hatchlings
#17
T Gao, M M Zhao, Y J Li, L Zhang, J L Li, L L Yu, F Gao, G H Zhou
This study was to investigate the effects of in ovo feeding (IOF) L-arginine (Arg) solution on the development of digestive organs, the duodenal mucosa of broiler embryos and hatchlings, and the growth performance of chicks during the first week post-hatch. A total of 720 fertilized eggs with similar weight were randomly allocated to three groups, consisting of eight replicates of 30 eggs each. Three treatments were arranged as non-injected control, diluent-injected (0.75% NaCl solution) group and Arg solution-injected group containing 1% Arg, dissolved in diluent...
May 15, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28503847/addition-of-color-doppler-sonography-for-detection-of-amniotic-fluid-disturbances-and-its-implications-on-perinatal-outcomes
#18
Imelda N Odibo, Brianna S Whittemore, Dawn S Hughes, Pamela M Simmons, Songthip T Ounpraseuth, Everett F Magann
OBJECTIVES: To determine whether color Doppler sonography increases the detection of pregnancies at risk for adverse outcomes. METHODS: Participants admitted to labor and delivery with the anticipation of a vaginal delivery underwent measurements of amniotic fluid volume (AFV) using amniotic fluid index (AFI) and single deepest pocket (SDP) techniques by grayscale followed by color Doppler sonography. Oligohydramnios was defined as an AFI of less than 5 cm or an SDP of less than 2 cm...
May 15, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28503779/normal-amniotic-fluid-volume-across-gestation-comparison-of-statistical-approaches-in-1190-normal-amniotic-fluid-volumes
#19
Songthip T Ounpraseuth, Everett F Magann, Horace J Spencer, Nader Z Rabie, Adam T Sandlin
AIM: Ultrasound estimation and evaluation of amniotic fluid volume (AFV) is an important component of pregnancy surveillance and fetal well-being. The purpose of this study was to compare and contrast four statistical methods used to construct gestational age-specific reference intervals for the assessment of AFV. METHODS: A total of 1095 normal AFV derived from four studies that measured AFV using dye-dilution or direct measurement at the time of hysterotomy were used to construct reference intervals using polynomial regression, quantile regression, Royston and Wright mean and SD, and Cole's lambda mu sigma (LMS) methods...
May 15, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28502201/prediction-of-chorioamnionitis-in-cases-of-intraamniotic-infection-by-ureaplasma-urealyticum-in-women-with-very-preterm-premature-rupture-of-membranes-or-preterm-labour
#20
Rocio Revello, María José Alcaide, Daniel Abehsera, María Martin-Camean, Mafalda Sousa E Faro Gomes, Bárbara Alonso-Luque, Jose L Bartha
OBJECTIVES: First, to determinate the frequency of chorioamnionitis and funisitis in cases of intramniotic detection of Ureaplasma urealyticum. And second, to assess the predictive capability of some biological markers in the amniotic fluid of these women to predict histological inflammation. SUBJECTS AND METHODS: We prospectively studied 20 cases of women with premature rupture of membranes or preterm labour (PROM) or preterm labour and intraamniotic detection of Ureaplasma urealyticum...
May 14, 2017: Journal of Maternal-fetal & Neonatal Medicine
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