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https://www.readbyqxmd.com/read/28542452/the-role-of-aspartyl-aminopeptidase-ape4-in-cryptococcus-neoformans-virulence-and-authophagy
#1
Fabiano de Assis Gontijo, Amanda Teixeira de Melo, Renata C Pascon, Larissa Fernandes, Hugo Costa Paes, J Andrew Alspaugh, Marcelo A Vallim
In order to survive and cause disease, microbial pathogens must be able to proliferate at the temperature of their infected host. We identified novel microbial features associated with thermotolerance in the opportunistic fungal pathogen Cryptococcus neoformans using a random insertional mutagenesis strategy, screening for mutants with defective growth at 37°C. Among several thermosensitive mutants, we identified one bearing a disruption in a gene predicted to encode the Ape4 aspartyl aminopeptidase protein...
2017: PloS One
https://www.readbyqxmd.com/read/28541836/description-of-candidatus-bartonella-fadhilae-n-sp-and-candidatus-bartonella-sanaae-n-sp-bartonellaceae-from-dipodillus-dasyurus-and-sekeetamys-calurus-gerbillinae-from-the-sinai-massif-egypt
#2
Mohammed Alsarraf, Eman M E Mohallal, Ewa J Mierzejewska, Jolanta Behnke-Borowczyk, Renata Welc-Falęciak, Małgorzata Bednarska, Lukasz Dziewit, Samy Zalat, Francis Gilbert, Jerzy M Behnke, Anna Bajer
Bartonella spp. are parasites of mammalian erythrocytes and endothelial cells, transmitted by blood-feeding arthropod ectoparasites. Different species of rodents may constitute the main hosts of Bartonella, including several zoonotic species of Bartonella. The aim of this study was to identify and compare Bartonella species and genotypes isolated from rodent hosts from the South Sinai, Egypt. Prevalence of Bartonella infection was assessed in rodents (837 Acomys dimidiatus, 73 Acomys russatus, 111 Dipodillus dasyurus, and 65 Sekeetamys calurus) trapped in 2000, 2004, 2008, and 2012 in four dry montane wadis around St...
May 25, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28538938/-analysis-of-the-geographical-distribution-of-cases-of-leprosy-rio-de-janeiro-2001-2012
#3
Renata Gracie, Julia Novaes de Barros Peixoto, Fabiane Bertoni Dos Reis Soares, Mariana de Andrea Vilas-Boas Hacker
Studies have demonstrated that the geographical distribution of leprosy is related to different socioeconomic factors. This article aims to study the geographical distribution of leprosy in the state of Rio de Janeiro. The cases of leprosy reported in the 2001-2012 period were mapped according to municipality. Epidemiological and socioeconomic indicators were calculated. The ArcMap program was used for the construction of maps and Earth View to calculate the Bayesian rate. It was observed that leprosy is presented in hyper-endemic levels especially in the metropolitan area...
May 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28538832/nasalance-scores-of-brazilian-portuguese-speakers-at-5-years-of-age
#4
Débora Natália de Oliveira, Ana Claudia Martins Sampaio-Teixeira, Bianca Gonçalves Alvarenga, Ana Paula Fukushiro, Renata Paciello Yamashita, Inge Elly Kiemle Trindade
Purpose: To determine nasalance scores of Brazilian Portuguese speaking children without evident speech disorders, language delay and orofacial deformities, at age 5 years, and analyze differences between types of speech samples and genders. Methods: Twenty children were analyzed, 11 males, age ranging from 4 years and 10 months to 5 years and 11 months. The Nasometer II 6450 (KayPENTAX) was used for nasalance assessment. Speech samples were eight consonant-vowel syllables and one sequence of nine words...
May 22, 2017: CoDAS
https://www.readbyqxmd.com/read/28538800/effect-of-aqueous-extract-of-the-leaves-of-baccharis-trimera-on-the-proliferation-of-hepatocytes-after-partial-hepatectomy-in-rats
#5
Sonia Oliveira Lima, Maria Bernadete Galrão de Almeida Figueiredo, Vanessa Rocha de Santana, Danillo Primo Andrade Santana, Matheus de Souza Nogueira, Erick Sobral Porto, Renata Lima Batalha de Andrade, Jessica Macêdo Santos, Ricardo Luiz Cavalcanti de Albuquerque, Juliana Cordeiro Cardoso
Purpose: To evaluate the effect of aqueous extract of Baccharis trimera leaves on the proliferative capacity of the liver after partial hepatectomy (PH) in rats. Methods: Twenty Wistar rats weighing between 300 and 450g were divided into two groups: control (HP) and test (HP100-rats that received the aqueous extract of Baccharis trimera for four days at a dose of 100 mg / kg / day). On the fifth day, animals from both groups underwent resection of 70% of the liver...
April 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28538786/routine-hiv-testing-in-men-who-have-sex-with-men-from-risk-to-prevention
#6
Bruna Robba Lara Redoschi, Eliana Miura Zucchi, Claudia Renata Dos Santos Barros, Vera Silvia Facciolla Paiva
We conducted a critical review of the literature on recurrent use of HIV testing in men who have sex with men (MSM). We performed a narrative review of the literature in which we analyzed the various conceptions on frequent testing over time, the implications for health programs, and the main social markers that influence the incorporation of HIV testing as routine care. Although it has existed since the 1990s, recurrent testing among MSM was frequently interpreted as increased exposure to HIV due to lack of condom use, and therefore as "unnecessary" testing...
May 18, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/28538301/proposal-of-a-new-specific-cardiopulmonary-exercise-test-for-taekwondo-athletes
#7
Marcus P Araujo, Antônio C L Nóbrega, Gabriel Espinosa, Matheus R Hausen, Renata R T Castro, Pedro P Soares, Jonas L Gurgel
Araujo, MP, Nóbrega, ACL, Espinosa, G, Hausen, MR, Castro, RRT, Soares, PP, and Gurgel, JL. Proposal of a new specific cardiopulmonary exercise test for taekwondo athletes. J Strength Cond Res 31(6): 1525-1535, 2017-The objective of this study is to evaluate the cardiorespiratory variables of Taekwondo athletes while performing incremental exercise test on an ergometer using a ramp protocol and to propose a specific protocol for assessing these physiological variables during Taekwondo practice. Fourteen athletes participated in 2 incremental exercise tests: a treadmill exercise test (TREADtest) and a Taekwondo-specific exercise test (TKDtest)...
June 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28536686/mandible-metastasis-of-small-cell-lung-cancer-mimicking-a-residual-cyst
#8
Renata Mendonça Moraes, Fabio Abreu Alves, Bruna Fernandes do Carmo Carvalho, Felipe D'Almeida Costa, Rodrigo Nascimento Lopes, Graziella Chagas Jaguar
Small cell lung carcinoma (SCLC) is an uncommon tumor characterized by an aggressive behavior with early metastasis, usually to the contralateral lung, liver, brain, and bones. There are only five cases of this particular tumor metastasizing to the oral cavity described in the English literature. We present the case of metastatic SCLC in the mandible with radiographic findings resembling a residual cyst. A 66-year-old man with previous diagnosis and treatment for a SCLC was referred to the Stomatology Department with a history of persistent pain in the mandible 1 year after the inferior right pre-molar tooth extraction...
January 2017: Autopsy & case reports
https://www.readbyqxmd.com/read/28535857/functional-characterization-of-the-collagen-binding-protein-dip2093-and-its-influence-on-host-pathogen-interaction-and-arthritogenic-potential-of-corynebacterium-diphtheriae
#9
Renata Stavracakis Peixoto, Camila Azevedo Antunes, Liliane Simpson Lourêdo, Vanilda Gonçalves Viana, Cintia Silva Dos Santos, Jemima Fuentes Ribeiro da Silva, Raphael Hirata, Elena Hacker, Ana Luíza Mattos-Guaraldi, Andreas Burkovski
Corynebacterium diphtheriae is typically recognized as the a etiological agent of diphtheria, a toxaemic infection of the respiratory tract; however, both non-toxigenic and toxigenic strains are increasingly isolated from cases of invasive infections. The molecular mechanisms responsible for bacterial colonization and dissemination to host tissues remain only partially understood. In this report, we investigated the role of DIP2093, described as a putative adhesin of the serine-aspartate repeat (Sdr) protein family in host-pathogen interactions of C...
May 23, 2017: Microbiology
https://www.readbyqxmd.com/read/28535679/triterpenoid-components-from-oak-heartwood-quercus-robur-and-their-potential-health-benefits
#10
Andy J Pérez, Łukasz Pecio, Mariusz Kowalczyk, Renata Kontek, Gabriela Gajek, Lidija Stopinsek, Ivan Mirt, Wiesław Oleszek, Anna Stochmal
For centuries oak wood (Quercus robur) have been used in aging of wines and spirits, which is based on pleasant flavors given to beverages by phenolics transferred to the liquid during the maturation process. Other metabolites like triterpenoids can also be released. Searching for extractable triterpenoids in oak heartwood, twelve new, 1-12, and five known, 13-17, oleanane types were isolated and characterized. Their cytotoxicities were tested against cancer cells (PC3 and MCF-7) and lymphocytes. Breast cancer cells (MCF-7) were the most affected by triterpenoids, roburgenic acid, 4, being the most active compound (IC50 = 19...
May 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28535094/rheological-and-physical-parameters-correlations-in-formulations-with-pinh%C3%A3-o-derivatives-stability-study-building-up-an-analytical-route
#11
Renata Moschini Daudt, Nilo Sergio Medeiros Cardozo, Ligia Damasceno Ferreira Marczak, Irene Clemes Külkamp Guerreiro
This study focuses on the correlations investigation between rheological and physical parameters and how it can contribute to optimize the topical formulations development. A gel and an emulgel containing pinhão derivatives, and their respective controls, were analyzed along six months of storage. A flowchart of analyses was proposed to use in topical formulation development when a benchmark is the goal or when it is necessary to change some raw material. All formulations were stable over the storage time and the formulations containing pinhão starch and coat extract presented similar properties to those of the control formulations...
May 23, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28534521/comprehensive-analysis-of-adverse-events-associated-with-per-oral-endoscopic-myotomy-in-1826-patients-an-international-multicenter-study
#12
Yamile Haito-Chavez, Haruhiro Inoue, Kristin W Beard, Peter V Draganov, Michael Ujiki, Burkhard H A Rahden, Pankaj N Desai, Mathieu Pioche, Bu Hayee, Amyn Haji, Payal Saxena, Kevin Reavis, Manabu Onimaru, Valerio Balassone, Jun Nakamura, Yoshitaka Hata, Dennis Yang, Davinderbir Pannu, Ali Abbas, Yaseen B Perbtani, Lava Y Patel, Jorg Filser, Sabine Roman, Jerome Rivory, Francois Mion, Thierry Ponchon, Silvana Perretta, Vivien Wong, Roberta Maselli, Saowanee Ngamruengphong, Yen-I Chen, Majidah Bukhari, Gulara Hajiyeva, Amr Ismail, Renata Pieratti, Vivek Kumbhari, Gerson Galdos-Cardenas, Alessandro Repici, Mouen A Khashab
OBJECTIVES: The safety of peroral endoscopic myotomy (POEM) is still debated since comprehensive analysis of adverse events (AEs) associated with the procedure in large multicenter cohort studies has not been performed. To study (1) the prevalence of AEs and (2) factors associated with occurrence of AEs in patients undergoing POEM. METHODS: Patients who underwent POEM at 12 tertiary-care centers between 2009 and 2015 were included in this case-control study. Cases were defined by the occurrence of any AE related to the POEM procedure...
May 23, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28533240/heterocycle-thiazole-compounds-exhibit-antifungal-activity-through-increase-in-the-production-of-reactive-oxygen-species-in-cryptococcus-neoformans-cryptococcus-gattii-species-complex
#13
Nívea Pereira de Sá, Caroline Miranda de Lima, Cleudiomar Inácio Lino, Paulo Jorge Sanches Barbeira, Ludmila de Matos Baltazar, Daniel Assis Santos, Renata Barbosa de Oliveira, Eleftherios Mylonakis, Beth Burgwyn Fuchs, Susana Johann
Human cryptococcosis can occur as a primary or opportunistic infection and develops as an acute, sub-acute, or chronic systemic infection, involving different organs of the host. Given the limited therapeutic options and the occasional resistance to fluconazole, there is a need to develop novel drugs for the treatment of cryptococcosis. In this report, we describe promising thiazoles 1, 2, 3, and 4 and explore their possible modes of action against Cryptococcus. To this end, we show evidence of interference in the Cryptococcus antioxidant system...
May 22, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28533011/effect-of-surface-treatments-on-repair-with-composite-resin-of-a-partially-monoclinic-phase-transformed-yttrium-stabilized-tetragonal-zirconia
#14
Beatriz Regalado Galvão Ribeiro, Marilia Regalado Galvão Rabelo Caldas, Antonio Alves Almeida, Renata Garcia Fonseca, Gelson Luis Adabo
STATEMENT OF PROBLEM: Studies of composite resin repairs of yttrium-tetragonal zirconia polycrystal (Y-TZP) are usually performed in its tetragonal phase, but it may be partially transformed into a monoclinic phase in a clinical fracture. PURPOSE: The purpose of this in vitro study was to evaluate the effect of airborne-particle abrasion (APA) and a bonding agent on the shear bond strength (SBS) between a composite resin and hydrothermally aged Y-TZP. MATERIAL AND METHODS: Specimens (7...
May 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28532839/dry-ice-is-a-reliable-substrate-for-the-distribution-of-frozen-mouse-spermatozoa-a-multi-centric-study
#15
Marcello Raspa, Mo Guan, Renata Paoletti, Lluis Montoliu, Abdel Ayadi, Susan Marschall, Martin Fray, Ferdinando Scavizzi
Disseminating mouse stocks as frozen materials offers both ethical and logistical advantages over live animal shipment, minimizing the welfare issues and avoiding some of the complex custom regulations that are associated with live animal transportation. Embryo freezing in liquid nitrogen (LN2) at -196 °C has traditionally been the method of choice for archiving mouse lines. However, spermatozoa freezing is emerging as a more convenient alternative due to the application of innovative cryopreservation and recovery protocols...
July 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28532809/seroprevalence-of-bovine-herpesvirus-1-related-alphaherpesvirus-infections-in-free-living-and-captive-cervids-in-poland
#16
Jerzy Rola, Magdalena Larska, Wojciech Socha, Jolanta G Rola, Magdalena Materniak, Renata Urban-Chmiel, Etienne Thiry, Jan F Żmudziński
To determine the occurrence of bovine herpesvirus 1 (BoHV-1) related alphaherpesvirus infections in cervids, 1194 serum samples of wild ruminants originating from 59 forest districts of Poland were tested with IBR gB ELISA and virus neutralization test (VNT) against BoHV-1 and cervid herpesvirus 1 (CvHV-1). The seroprevalence differed significantly between free-living and captive cervids (P<0.001) with a total of 89 out of 498 (17.9%) and 268 out of 696 (38.5%) seropositive animals in each type of population...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28532027/evaluation-of-different-surface-treatments-on-fiber-post-cemented-with-a-self-adhesive-system
#17
Maíra Prado, Juliana N Marques, Gisele Damiana Pereira, Eduardo M da Silva, Renata A Simão
Surface treatment of fiber-reinforced posts can increase adhesion, especially on the post/resin cement interface. The purpose of this in vitro study was to evaluate the effect of different surface treatments on fiber post cemented with a self-adhesive system. Sixty fiberglass epoxy resin posts were cleaned, dried and divided into 6 groups (n=10): Control (no surface treatment), silane (silane coupling agent was applied homogeneously on surface), 24% hydrogen peroxide (H2O2) (immersion during 1min), blasting (blasting with aluminum oxide for 30s), NH3 plasma (plasma treatment for 3min) and HMDSO plasma (plasma treatment for 15min)...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531990/safety-protocol-for-the-gold-nanoparticles-administration-in-rats
#18
Alexandre Pastoris Muller, Gabriela Kozuchovski Ferreira, Sabrina da Silva, Renata Tiscoski Nesi, Gustavo de Bem Silveira, Carolini Mendes, Ricardo A Pinho, Marcos Marques da Silva Paula, Paulo Cesar Lock Silveira
Gold nanoparticles (GNPs) have antioxidant and anti-inflammatory effects. However, toxicity is still a concern; therefore, it is critical to study both the therapeutic and toxic properties of GNPs. In this study, we evaluated the effects of the intraperitoneal administration of GNPs (20nm, at a concentration of 2.5mg/L for 21days) every 24 or 48h on oxidative stress, antioxidant status, and electron chain transport (ETC) in the brain. Liver histology and blood marker analyses were conducted to establish a time routine of GNP administration...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531811/synthesis-of-novel-indole-derivatives-as-promising-dna-binding-agents-and-evaluation-of-antitumor-and-antitopoisomerase-i-activities
#19
Elizabeth Almeida Lafayette, Sinara Mônica Vitalino de Almeida, Renata Virginia Cavalcanti Santos, Jamerson Ferreira de Oliveira, Cezar Augusto da Cruz Amorim, Rosali Maria Ferreira da Silva, Maira Galdino da Rocha Pitta, Ivan da Rocha Pitta, Ricardo Olimpio de Moura, Luiz Bezerra de Carvalho Júnior, Moacyr Jesus Barreto de Melo Rêgo, Maria do Carmo Alves de Lima
Molecules bearing indole nucleus present diverse biological properties such as antitumor and anti-inflammatory activities that can be associated both to DNA and protein interactions. This study focused on the synthesis of new indole derivatives with thiazolidines and imidazolidine rings condensed as side chains as well as the evaluation of their ability to interact with the DNA and antitumor and topoisomerase inhibition activities. All derivatives were successfully synthesized and their structures were elucidated by mass spectrometry (MS), infrared (IR), spectroscopy (1)H NMR, (13)C NMR, COSY (1)H-(1)H and HSQC (1)H-(13)C...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28529953/bis-4-4-dimethyl-2-2-bipyridine-oxidovanadium-iv-sulfate-dehydrate-potential-candidate-for-controlling-lipid-metabolism
#20
Renata Francik, Jadwiga Kryczyk-Kozioł, Sławomir Francik, Ryszard Gryboś, Mirosław Krośniak
Vanadium is a trace element mainly connected with regulation of insulin metabolism which is particularly important in diabetes. In recent years, organic complexes of vanadium seem to be more interesting than inorganic salts. Nevertheless, the effect of vanadium on lipid metabolism is still a problematic issue; therefore, the main purpose of this study was to investigate the effect of 3 organic complexes of vanadium such as sodium (2,2'-bipyridine)oxidobisperoxovanadate(V) octahydrate, bis(2,2'-bipyridine)oxidovanadium(IV) sulfate dehydrate, and bis(4,4'-dimethyl-2,2'-bipyridine)oxidovanadium(IV) sulfate dihydrate in conjunction with high-fat as well as control diet in nondiabetes model on the following lipid parameters: total cholesterol, triglycerides, and high density lipoprotein as well as activity of paraoxonase 1...
2017: BioMed Research International
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