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R X Deng, X H Ren, J W Ruan, J Zheng, J C Zhong, W X Lu, X Q Zou, J J Liu
Objective: To further explore TCE-induced hepatotoxicity and its mechanisms by identification of trichloroethylene (TCE) induced abnormal histone methylation in human liver cells. Methods: L-02 cells were treated with 0 and 8 mmol/L TCE for 24 h. Histones were extracted by acid. Liquid chromatography electrospray ionization tandem mass spectrometry (ESI-LC-MS/MS) were used to identify and quantify TCE related histone methylations. TCE induced abnormal methylation of H3K79 me2 and H3K79 me3 were validated by Western blot analysis...
April 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
Mohd Akbar Bhat, Gursatej Gandhi
DNA damage in the peripheral blood leukocytes (PBL) of patients with coronary artery disease (CAD) was investigated using the sensitive alkaline single cell gel electrophoresis (SCGE)/comet assay.This case-control study consisted of CAD patients (n = 200; mean age, 59.04 ± 0.75 years) undergoing treatment at local hospitals and age-, sex-, and ethnicity-matched healthy controls (n = 200; mean age, 57.88 ± 0.96 years) from the general population.CAD patients had significantly (P < 0.001) increased DNA damage (tail DNA percent (T-DNA %) 22...
March 17, 2017: International Heart Journal
Andrew G L Douglas, Gaia Andreoletti, Kevin Talbot, Simon R Hammans, Jaspal Singh, Andrea Whitney, Sarah Ennis, Nicola C Foulds
We describe a family with an autosomal dominant familial dyskinesia resembling myoclonus-dystonia associated with a novel missense mutation in ADCY5, found through whole-exome sequencing. A tiered analytical approach was used to analyse whole-exome sequencing data from an affected grandmother-granddaughter pair. Whole-exome sequencing identified 18,000 shared variants, of which 46 were non-synonymous changes not present in a local cohort of control exomes (n = 422). Further filtering based on predicted splicing effect, minor allele frequency in the 1000 Genomes Project and on phylogenetic conservation yielded 13 candidate variants, of which the heterozygous missense mutation c...
April 2017: Neurogenetics
Noelia Nikoloff, Ana M Pascua, Juan M Anchordoquy, Juan P Anchordoquy, Matías A Sirini, Analia Seoane, Cecilia C Furnus
The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, and 1 mM EPA. The effect of EPA was evaluated on cumulus-oocyte complexes (COC) by oocyte maturation (cumulus expansion area and oocyte nuclear maturation), genotoxicity [single cell gel electrophoresis (SCGE)], and cytotoxicity (apoptosis, viability, and MTT assays) end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVM medium...
May 2017: Cell Biology International
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Shu-Ying Yu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification...
2017: Frontiers in Microbiology
Sema Oncul, Lale Karabiyik, Erdem Coskun, Ela Kadioglu, Ozlem Gulbahar
BACKGROUND AND OBJECTIVES: The aim of this study was to compare the effects of sevoflurane and propofol anesthesia on oxidative DNA damage that occurs in low-extremity ischemia and is caused by tourniquet application. METHODS: Fourteen New Zealand rabbits were randomly allocated into two equal groups. Group S (n=7) received sevoflurane (2.5-4 percent) inhalation and Group P (n=7) received a propofol infusion (1-2mg·kg(-1)·min(-1)), after which a pneumatic tourniquet was placed on the right lower extremity...
January 2017: Brazilian Journal of Anesthesiology
Shradha Subedi, Fanrong Kong, Peter Jelfs, Timothy J Gray, Meng Xiao, Vitali Sintchenko, Sharon C-A Chen
Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capillary gel electrophoresis (SCGE). Fourteen American Type Culture Collection (ATCC) strains and 103 clinical/environmental isolates (total n = 24 species) of SG-NTM were included...
2016: PloS One
Hatice Tunca, Ahmet Ali Berber, Kubra Çanakçi, Murat Tuna, Salih Zeki Yildiz, Hüseyin Aksoy
In this study, a Cd(II) complex was synthesized using 8-hydroxyquinoline and thiocyanate as the ligands and structurally characterized with the combination of FTIR, (1)H-NMR, (13)C-NMR, UV-vis, and MS spectral data. Then, genotoxic effects of the prepared complex were investigated. Genotoxic properties of the dimeric 8-hydroxyquinolinthiocyanatoCd(II) [Cd2(8Q)2(SCN)2] complex synthesized as drug raw material were analyzed in human peripheral blood lymphocytes. Concentrations of 1, 2, 4, 6, and 8 μg/mL [Cd2(8Q)2(SCN)2] were used for 24 and 48  h durations...
September 15, 2016: Drug and Chemical Toxicology
Elena Kuzminsky, Roberta Meschini, Serena Terzoli, Liliana Pavani, Cristian Silvestri, Zineb Choury, Giuseppe Scarascia-Mugnozza
Abiotic stresses have considerable negative impact on Mediterranean plant ecosystems and better comprehension of the genetic control of response and adaptation of trees to global changes is urgently needed. The single cell gel electrophoresis (SCGE) assay could be considered a good estimator of DNA damage in an individual eukaryotic cell. This method has been mainly employed in animal tissues, because the plant cell wall represents an obstacle for the extraction of nuclei; moreover, in Mediterranean woody species, especially in the sclerophyll plants, this procedure can be quite difficult because of the presence of sclerenchyma and hardened cells...
2016: Frontiers in Plant Science
Elanur Aydin, Şener Taşdemir, Fazıl Hacımüftüoğlu, Hasan Türkez
Thymol (THY), which is a monocyclic monoterpene, is one of the major constituent of the essential oils of thyme and origanum. To date, although various biological activities of THY have been demonstrated, its neurotoxicity has never been explored. We aimed to describe in vitro antiproliferative and/or cytotoxic properties (by 3-(4,5 dimetylthiazol-2-yl)-2,5 diphenlytetrazolium bromide (MTT) test), genotoxic damage potentials (by single cell gel electrophoresis (SCGE)) and oxidative effects (by total antioxidant capacity (TAC) and total oxidative stress (TOS) analysis) of THY...
August 23, 2016: Central Nervous System Agents in Medicinal Chemistry
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Jing-Wei Cheng, Xin-Xin Chen, Zhi-Peng Xu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida haemulonii complex (Candida haemulonii, Candida haemulonii var. vulnera, and Candida duobushaemulonii) consists of emerging pathogens. Thirty-one isolates from 14 hospitals in China were studied for their species classification and antifungal susceptibilities. Performances of molecular (i.e., ribosomal DNA [rDNA] internal transcribed spacer [ITS] sequencing, D1/D2 sequencing, and ITS sequencer-based capillary gel electrophoresis [SCGE]) and phenotypic identification methods in species identification were compared...
November 2016: Journal of Clinical Microbiology
Mohammad Salimi-Asl, Hossein Mozdarani, Mehdi Kadivar
INTRODUCTION: Polycystic ovary syndrome (PCOS), a multigenic endocrine disorder, is highly associated with low-grade chronic inflammation, however its etiology remains unclear. In this study, we employed dehydroepiandrosterone (DHEA)-treated mice to reveal the molecular mechanism of inflammation and its correlation with oxidative stress in PCOS patients. METHODS: miR-21 and miR-146a expression levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR)...
2016: BioImpacts: BI
Marco Parolini, Sara Castiglioni, Stefano Magni, Camilla Della Torre, Andrea Binelli
Cannabis is the most used illicit drug worldwide and in some countries a new regulatory policy makes it legal under some restrictions. This situation could lead to a substantial increase in environmental levels of the cannabis active principle (Δ-9-tetrahydrocannabinol [Δ-9-THC]) and its main metabolite, 11-nor-9-carboxy-Δ(9) -tetrahydrocannabinol (THC-COOH). Although previous studies have highlighted the toxicity of Δ-9-THC, the adverse effects of THC-COOH on aquatic organisms is completely unknown, even though such effects could be more significant because the environmental concentrations of THC-COOH are higher than those of the parent compound...
October 5, 2016: Environmental Toxicology and Chemistry
Marcos Mateo-Fernández, Tania Merinas-Amo, Miguel Moreno-Millán, Ángeles Alonso-Moraga, Sebastián Demyda-Peyrás
The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola™ (CCC) and Caffeine-Free Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model...
2016: BioMed Research International
Wenshuang Wu, Buyun Ma, Haoyu Ye, Taijin Wang, Xiaoyan Wang, Jianhong Yang, Yuquan Wei, Jingqiang Zhu, Lijuan Chen
Millepachine (MIL) was a novel chalcone that was separated from Millettia pachycarpa Benth (Leguminosae). We found MIL induced apoptosis through activating NF-κB pathway both in SK-OV-3 and A2780S cells. Western blot showed that MIL increased the levels of IKKα, p-IKKα/β, p-IκBα and NF-κB (p65) proteins, and decreased the expression of IκBα protein. Immunohistochemistry analysis indicated that translocation of NF-κB into the nucleus increased in both ovarian cancer cells. EMSA assay proved MIL enhanced NF-κB DNA-binding activity in the nuclear...
August 9, 2016: Oncotarget
Teresa T Duncan, Barbara H Berrie, Richard G Weiss
We report the influence of adding five short-chain glycol ethers (SCGEs) on the structure, stability, and viscoelastic properties of aqueous dispersions of partially hydrolyzed poly(vinyl acetate) and borax. The properties of these gel-like materials have been investigated as a function of the structure of the added SCGE both below and above the critical aggregation (or micellar) concentrations using (11) B and (13) C NMR, rheology, and small-angle neutron scattering. The results indicate that the SCGE aggregation behavior is not affected by incorporation into the gel-like network...
August 18, 2016: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Juan José Rodríguez-Mercado, Diana Florín-Ramírez, Lucila Álvarez-Barrera, Mario Agustín Altamirano-Lozano
A variety of metal ions have biological functions, and many researchers have not actively investigated copper compounds, based on the assumption that endogenous metals might be less toxic. In the present study, we used a dual fluorochrome method and a single cell gel electrophoresis (SCGE) assay at pH > 13 to evaluate the cell viability and DNA damage induced by a copper-based antineoplastic drug, Casiopeina II-gly®, at concentrations of 1.04, 2.08, 4.17, 8.35 or 16 μg/mL in human peripheral-blood leukocytes in vitro...
April 2017: Drug and Chemical Toxicology
Sonia Soloneski, Celeste Ruiz de Arcaute, Marcelo L Larramendy
The acute toxicity of two herbicide formulations, namely, the 57.71 % dicamba (DIC)-based Banvel(®) and the 48 % glyphosate (GLY)-based Credit(®), alone as well as the binary mixture of these herbicides was evaluated on late-stage Rhinella arenarum larvae (stage 36) exposed under laboratory conditions. Mortality was used as an endpoint for determining acute lethal effects, whereas the single-cell gel electrophoresis (SCGE) assay was employed as genotoxic endpoint to study sublethal effects. Lethality studies revealed LC5096 h values of 358...
September 2016: Environmental Science and Pollution Research International
Minor P Huffman, Anja H Høie, Camilla Svendsen, Gunnar Brunborg, Michael Murkovic, Hansruedi Glatt, Trine Husøy
2,5-Dimethylfuran (DMF) and furfuryl alcohol (FFA) are two substituted furans that are formed during the processing of foods and have also been used as food flavorings. DMF and FFA are proposed to be bioactivated by human sulfotransferases (SULTs) which are not expressed in conventional cell lines used for genotoxicity testing. Therefore, in addition to the standard V79 cell line, we used a transfected V79 derived cell line co-expressing human cytochrome P450 (CYP) 2E1 and human SULT1A1 to assess the genotoxicity of DMF and FFA...
September 2016: Mutagenesis
Taixiu Liu, Jianfang Gao, Niya Zhou, Min Mo, Xiaogang Wang, Xi Zhang, Huan Yang, Qing Chen, Lin Ao, Jinyi Liu, Zhihong Cui, Jia Cao
Several methods are currently available for selection when conducting sperm cryopreservation, however, these methods might cause different degrees of damage on sperm DNA. The aim of the this study is to compare the effects of storage at -80 °C (in ultra-low temperature refrigerator) and at -196 °C (in liquid nitrogen) on sperm DNA damage, thus to provide a reference for choosing the right method according to different aims. We randomly collected 28 semen samples from college students of Chongqing city...
2016: Cryobiology
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